Method for cultivating ganoderma lucidum by using automatic temperature control system

Abstract

The invention belongs to the technical field of meat lucid ganoderma, particularly relates to a method for cultivating meat lucid ganoderma by using an automatic temperature control system, and provides the following scheme aiming at the existing problems that pesticide residues are easy to occur and heavy metals exceed the standard: S1, obtaining meat lucid ganoderma, a culture solution and an automatic temperature control box, the culture solution comprises the following components in parts by weight: 1-5% of corn flour, 1-3% of soybean meal, 0.2-1.0% of beef, 5-10ppm of metal powder, 0.2-1.0% of lime powder, 1-5% of ceramic particles, 1-3% of quartz sand and the balance of water; s2, corn flour, soybean meal, beef, metal powder, lime powder, ceramic particles, quartz sand and water are obtained and mixed to be uniform, then the mixture is added into a pressure cooker to be boiled, through cooperation of a culture solution and an automatic temperature control box, the meat lucid ganoderma is effectively cultivated, residues of pesticide and heavy metal in the meat lucid ganoderma can be avoided, and the purity of the meat lucid ganoderma is guaranteed.

Description

Technical Field

[0001] The present invention relates to the technical field of Ganoderma lucidum, and in particular to a method for cultivating Ganoderma lucidum by utilizing an automatic temperature control system. Background Art

[0002] The Chinese patent document with application number 201911212873.8 discloses a method for cultivating meat Ganoderma lucidum. The mother meat Ganoderma lucidum is placed in an aqueous solution, the water temperature is controlled between 13℃ and 15℃, and placed for 7 to 10 days, and then coarsely filtered to obtain a slime mold fission cell solution for standby; the mother meat Ganoderma lucidum is replaced with a new aqueous solution, the temperature is controlled between 18℃ and 26℃, and a fungal spore and bacterial propagule solution is obtained; the slime mold fission cell solution is mixed with the fungal spore and bacterial propagule solution, and food-grade cold gel is added to the mixed solution at the same time. The slime mold, fungus and bacteria in the artificially synthesized gel meat Ganoderma lucidum continue to generate and gradually become the natural meat Ganoderma lucidum. The method of the present invention realizes the artificial domestication of wild meat Ganoderma lucidum and awakens the growth characteristics of wild meat Ganoderma lucidum; thereby laying the foundation for its transformation into normal production as soon as possible, which is conducive to the large-scale production and application of meat Ganoderma lucidum. The formed meat Ganoderma lucidum thalli is bright in color, good in shape, rich in nutrition and excellent in quality.

[0003] However, there are also some problems with the cultivation method of the Ganoderma lucidum. For example, during its production process, it will come into contact with pesticides to a greater or lesser extent, resulting in pesticide residues in the Ganoderma lucidum. In addition, the Ganoderma lucidum produced by it will more or less contain a small amount of heavy metals. Summary of the invention

[0004] Based on the problem that pesticide residues and heavy metals exceeding the standard are prone to occur in the background technology, the present invention proposes a method for cultivating Ganoderma lucidum by using an automatic temperature control system.

[0005] The present invention proposes a method for cultivating Ganoderma lucidum by using an automatic temperature control system, comprising the following steps:

[0006] S1: Obtain meat ganoderma, culture solution and automatic temperature control box, wherein the culture solution comprises by weight 1% to 5% corn flour, 1% to 3% soybean flour, 0.2% to 1.0% beef, 5ppm to 10ppm metal powder, 0.2% to 1.0% lime powder, 1% to 5% ceramic particles, 1% to 3% quartz sand and the balance water;

[0007] S2: Obtain corn flour, soybean flour, beef, metal powder, lime powder, ceramic particles, quartz sand and water, mix them evenly, add them into a pressure cooker to boil, then naturally cool them to 30°C, pour them into a fermentation tank, add yeast for fermentation, and after the fermentation is completed, sterilize them to obtain a culture solution;

[0008] S3: Pour the culture medium into a sterile container, then put the Ganoderma lucidum to be cultivated, then put the sterile container into an automated temperature control box, and start cultivating Ganoderma lucidum using an automated temperature control system;

[0009] S4: After 3 days of cultivation, start stirring the inside of the sterile container twice a day. After 8 to 12 days of cultivation, stop stirring and continue cultivating for 3 to 5 months to obtain Ganoderma lucidum. Use corn flour, soybean flour, beef, metal powder, lime powder, ceramic particles, quartz sand and water to prepare culture medium. Use the culture medium and an automated temperature control box to effectively cultivate Ganoderma lucidum, avoid the presence of pesticides and heavy metal residues in Ganoderma lucidum, and ensure the purity of Ganoderma lucidum.

[0010] Preferably, in S1, the metal powder includes treated iron powder and treated zinc powder, and the weight ratio of the treated iron powder to the treated zinc powder is 3:1, which is convenient for subsequent processing.

[0011] Preferably, the method for processing the iron powder is as follows: first, the iron powder to be processed is added to a grinding device for grinding to obtain iron powder that passes 200 meshes, then the iron powder is added to ethanol, ultrasonic waves are started to clean the iron powder, the iron powder is taken out after 5 minutes, and it is poured into distilled water, and ultrasonic waves are started again for processing to remove impurities and oil stains on the surface of the iron powder to ensure that the surface of the iron powder is clean, and then the iron powder is screened to remove particles that are too large or too small, and iron powder with a relatively uniform particle size is selected to better control the addition amount and the uniformity of the reaction. In this process, 200 mesh and 250 mesh sieves can be used for screening respectively to obtain iron powder that passes the 200 mesh sieve but not the 250 mesh sieve, and then the cleaned and screened iron powder is placed in the air for appropriate oxidation for a period of time to form a stable iron oxide layer on its surface to reduce the activity of the iron powder, and finally the treated iron powder is analyzed for composition to ensure that no other harmful impurities are introduced, and finally the treated iron powder is obtained, which can prevent the iron powder from affecting the production of Ganoderma lucidum and supplement iron ions.

[0012] Preferably, the method for processing the zinc powder is as follows: first, impurities in the zinc powder need to be removed, the zinc powder is screened with a 200-mesh screen to remove larger particles of impurities and lumps, the zinc powder is washed three times with deionized water, during the washing process, the washing liquid is removed by filtering to remove dust and other water-soluble impurities that may be attached to the surface of the zinc powder, then, the zinc powder needs to be converted into a suitable form, the zinc powder is dissolved with dilute hydrochloric acid to form a zinc ion solution, the zinc ions are precipitated in the form of zinc hydroxide compounds by adjusting the pH value and other methods, the precipitate is washed and dried to obtain treated zinc powder, which is convenient for the Ganoderma lucidum to absorb the zinc powder and supplement nutrients.

[0013] Preferably, in S1, the preparation method of lime powder is as follows: lime is obtained, poured into a grinder for preliminary grinding, coarse lime powder passing through a 200-mesh sieve is obtained, and finally the lime powder is processed again using a jet milling method to obtain lime powder, which can avoid the lime powder from hindering the growth of Ganoderma lucidum and is beneficial to the growth of Ganoderma lucidum.

[0014] Preferably, in S1, the method for processing ceramic particles: first, the particle size of the ceramic particles is screened to pass through a 50-mesh sieve, and then the ceramic particles are thoroughly cleaned with deionized water to remove impurities and contaminants on the surface of the ceramic particles, and then high-temperature sterilization is performed to ensure that the ceramic particles are sterile, and finally surface treatment is performed to introduce bioactive molecules on the surface of the ceramic particles through a chemical modification method, thereby obtaining ceramic particles, which is beneficial to the cultivation of Ganoderma lucidum by ceramic particles.

[0015] Preferably, in S2, the stirring speed is controlled at 120 r / min to 180 r / min, and the stirring time is controlled at 10 minutes to 15 minutes to enhance the mixing effect.

[0016] Preferably, in S2, during the fermentation process, the raw materials inside the fermentation tank are continuously stirred, and the fermentation is carried out for 12 hours to 24 hours to ensure the fermentation effect.

[0017] Preferably, in S3, the cultivation is carried out at a temperature of 28°C to 33°C, which can ensure the effect of the cultivation and prevent the growth of Ganoderma lucidum from being affected by too high or too low temperature.

[0018] Preferably, in S4, after taking out the Ganoderma lucidum, the residual material in the sterile container is filtered to obtain the Ganoderma lucidum culture solution and the Ganoderma lucidum culture residue, and the Ganoderma lucidum culture solution and the Ganoderma lucidum culture residue can be recycled later to avoid waste of resources.

[0019] Beneficial effects of the present invention:

[0020] The culture medium is prepared using corn flour, soybean flour, beef, metal powder, lime powder, ceramic particles, quartz sand and water. The culture medium is used in conjunction with an automated temperature control box to effectively cultivate the Ganoderma lucidum. This also prevents the presence of pesticides and heavy metal residues in the Ganoderma lucidum, thereby ensuring its purity. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 The working flow chart proposed by the present invention. DETAILED DESCRIPTION

[0022] The present invention will be further explained below in conjunction with specific embodiments.

[0023] Reference Figure 1 , Embodiment 1

[0024] This embodiment proposes a method for cultivating Ganoderma lucidum using an automated temperature control system, comprising the following steps:

[0025] S1: Obtain meat ganoderma lucidum, culture solution and automatic temperature control box, the weight proportion of the culture solution includes 4% corn flour, 2% soybean flour, 0.9% beef, 6ppm metal powder, 0.4% lime powder, 4% ceramic particles, 3% quartz sand and the balance water, the metal powder includes treated iron powder and treated zinc powder, the weight ratio of the treated iron powder to the treated zinc powder is 3:1, the processing method of iron powder is as follows: firstly, the iron powder to be treated is added into the grinding equipment for grinding to obtain the iron powder with a mesh size of 200, then the iron powder is added into ethanol, the ultrasonic wave is started to clean the iron powder, the iron powder is taken out after 5 minutes, and the iron powder is Pour in distilled water and start ultrasonic treatment again to remove impurities and oil on the surface of the iron powder to ensure the cleanliness of the iron powder surface. Then screen the iron powder to remove particles that are too large or too small, and select iron powder with a relatively uniform particle size to better control the amount added and the uniformity of the reaction. In this process, you can use 200-mesh and 250-mesh sieves for screening respectively, and obtain iron powder that passes the 200-mesh sieve but not the 250-mesh sieve. Then place the cleaned and screened iron powder in the air for a period of time to properly oxidize it so that a stable iron oxide layer is formed on its surface to reduce the activity of the iron powder. Finally, analyze the composition of the treated iron powder to confirm Ensure that no other harmful impurities are introduced, and finally obtain the treated iron powder. The treatment method of zinc powder: First, it is necessary to remove impurities in the zinc powder. Use a 200-mesh sieve to screen the zinc powder to remove larger particles of impurities and agglomerates. Wash the zinc powder three times with deionized water. During the washing process, remove the cleaning liquid by filtering to remove dust and other water-soluble impurities that may be attached to the surface of the zinc powder. Then, the zinc powder needs to be converted into a suitable form. Use dilute hydrochloric acid to dissolve the zinc powder to form a zinc ion solution. By adjusting the pH value and other methods, the zinc ions are precipitated in the form of zinc hydroxide compounds. The precipitate is washed, Drying to obtain treated zinc powder, obtaining lime, pouring it into the inside of a grinder for preliminary grinding, obtaining coarse lime powder passing through a 200-mesh sieve, and finally using a jet milling method for further processing to obtain lime powder. The processing method of ceramic particles is as follows: firstly, the particle size of the ceramic particles is screened to pass through a 50-mesh sieve, and then the ceramic particles are fully washed with deionized water to remove impurities and contaminants on the surface of the ceramic particles, and then high-temperature sterilization is performed to ensure that the ceramic particles are sterile, and finally surface treatment is performed to introduce bioactive molecules on the surface of the ceramic particles through a chemical modification method to obtain ceramic particles;

[0026] S2: Obtain corn flour, soybean flour, beef, metal powder, lime powder, ceramic particles, quartz sand and water, control the stirring speed at 1580r / min, control the stirring time at 12 minutes, mix evenly, add to a pressure cooker and boil, then naturally cool to 30°C, pour into a fermentation tank, and add yeast for fermentation. After the fermentation is completed, sterilize to obtain a culture solution. During the fermentation process, continuously stir the raw materials in the fermentation tank for 17 hours;

[0027] S3: Pour the culture medium into a sterile container, then put the Ganoderma lucidum to be cultivated, then put the sterile container into an automated temperature control box, use the automated temperature control system to start cultivating the Ganoderma lucidum, and cultivate it at a temperature of 28°C to 33°C.

[0028] S4: After 3 days of cultivation, the inside of the sterile container was stirred twice a day. After 11 days of cultivation, stirring was stopped and cultivation was continued for 4 months to obtain Ganoderma lucidum. After taking out the Ganoderma lucidum, the residual material in the sterile container was filtered to obtain Ganoderma lucidum culture solution and Ganoderma lucidum culture residue.

[0029] Reference Figure 1 , Embodiment 2

[0030] This embodiment proposes a method for cultivating Ganoderma lucidum using an automated temperature control system, comprising the following steps:

[0031] S1: Obtain meat ganoderma lucidum, culture solution and automatic temperature control box, the weight proportion of the culture solution includes 4% corn flour, 3% soybean flour, 0.9% beef, 9ppm metal powder, 0.7% lime powder, 4% ceramic particles, 3% quartz sand and the balance water, the metal powder includes treated iron powder and treated zinc powder, the weight ratio of the treated iron powder to the treated zinc powder is 3:1, the processing method of the iron powder is as follows: firstly, the iron powder to be treated is added into the grinding equipment for grinding to obtain the iron powder with a mesh size of 200, then the iron powder is added into ethanol, the ultrasonic wave is started to clean the iron powder, the iron powder is taken out after 5 minutes, and the iron powder is Pour in distilled water and start ultrasonic treatment again to remove impurities and oil on the surface of the iron powder to ensure the cleanliness of the iron powder surface. Then screen the iron powder to remove particles that are too large or too small, and select iron powder with a relatively uniform particle size to better control the amount added and the uniformity of the reaction. In this process, you can use 200-mesh and 250-mesh sieves for screening respectively, and obtain iron powder that passes the 200-mesh sieve but not the 250-mesh sieve. Then place the cleaned and screened iron powder in the air for a period of time to properly oxidize it so that a stable iron oxide layer is formed on its surface to reduce the activity of the iron powder. Finally, analyze the composition of the treated iron powder to confirm Ensure that no other harmful impurities are introduced, and finally obtain the treated iron powder. The treatment method of zinc powder: First, it is necessary to remove impurities in the zinc powder. Use a 200-mesh sieve to screen the zinc powder to remove larger particles of impurities and agglomerates. Wash the zinc powder three times with deionized water. During the washing process, remove the cleaning liquid by filtering to remove dust and other water-soluble impurities that may be attached to the surface of the zinc powder. Then, the zinc powder needs to be converted into a suitable form. Use dilute hydrochloric acid to dissolve the zinc powder to form a zinc ion solution. By adjusting the pH value and other methods, the zinc ions are precipitated in the form of zinc hydroxide compounds. The precipitate is washed, Drying to obtain treated zinc powder, obtaining lime, pouring it into the inside of a grinder for preliminary grinding, obtaining coarse lime powder passing through a 200-mesh sieve, and finally using a jet milling method for further processing to obtain lime powder. The processing method of ceramic particles is as follows: firstly, the particle size of the ceramic particles is screened to pass through a 50-mesh sieve, and then the ceramic particles are fully washed with deionized water to remove impurities and contaminants on the surface of the ceramic particles, and then high-temperature sterilization is performed to ensure that the ceramic particles are sterile, and finally surface treatment is performed to introduce bioactive molecules on the surface of the ceramic particles through a chemical modification method to obtain ceramic particles;

[0032] S2: Obtain corn flour, soybean flour, beef, metal powder, lime powder, ceramic particles, quartz sand and water, control the stirring speed at 1580r / min, control the stirring time at 12 minutes, mix evenly, add to a pressure cooker and boil, then naturally cool to 30°C, pour into a fermentation tank, and add yeast for fermentation. After the fermentation is completed, sterilize to obtain a culture solution. During the fermentation process, continuously stir the raw materials in the fermentation tank for 17 hours;

[0033] S3: Pour the culture medium into a sterile container, then put the Ganoderma lucidum to be cultivated, then put the sterile container into an automated temperature control box, use the automated temperature control system to start cultivating the Ganoderma lucidum, and cultivate it at a temperature of 28°C to 33°C.

[0034] S4: After 3 days of cultivation, the inside of the sterile container was stirred twice a day. After 11 days of cultivation, stirring was stopped and cultivation was continued for 4 months to obtain Ganoderma lucidum. After taking out the Ganoderma lucidum, the residual material in the sterile container was filtered to obtain Ganoderma lucidum culture solution and Ganoderma lucidum culture residue.

[0035] Reference Figure 1 , Embodiment 3

[0036] This embodiment proposes a method for cultivating Ganoderma lucidum using an automated temperature control system, comprising the following steps:

[0037] S1: Obtain meat ganoderma lucidum, culture solution and automatic temperature control box, the weight proportion of the culture solution includes 4% corn flour, 3% soybean flour, 0.7% beef, 8ppm metal powder, 0.6% lime powder, 4% ceramic particles, 3% quartz sand and the balance water, the metal powder includes treated iron powder and treated zinc powder, the weight ratio of the treated iron powder to the treated zinc powder is 3:1, the processing method of iron powder is as follows: firstly, the iron powder to be treated is added into the grinding equipment for grinding to obtain the iron powder with a mesh size of 200, then the iron powder is added into ethanol, the ultrasonic wave is started to clean the iron powder, the iron powder is taken out after 5 minutes, and the iron powder is Pour in distilled water and start ultrasonic treatment again to remove impurities and oil on the surface of the iron powder to ensure the cleanliness of the iron powder surface. Then screen the iron powder to remove particles that are too large or too small, and select iron powder with a relatively uniform particle size to better control the amount added and the uniformity of the reaction. In this process, you can use 200-mesh and 250-mesh sieves for screening respectively, and obtain iron powder that passes the 200-mesh sieve but not the 250-mesh sieve. Then place the cleaned and screened iron powder in the air for a period of time to properly oxidize it so that a stable iron oxide layer is formed on its surface to reduce the activity of the iron powder. Finally, analyze the composition of the treated iron powder to confirm Ensure that no other harmful impurities are introduced, and finally obtain the treated iron powder. The treatment method of zinc powder: First, it is necessary to remove impurities in the zinc powder. Use a 200-mesh sieve to screen the zinc powder to remove larger particles of impurities and agglomerates. Wash the zinc powder three times with deionized water. During the washing process, remove the cleaning liquid by filtering to remove dust and other water-soluble impurities that may be attached to the surface of the zinc powder. Then, the zinc powder needs to be converted into a suitable form. Use dilute hydrochloric acid to dissolve the zinc powder to form a zinc ion solution. By adjusting the pH value and other methods, the zinc ions are precipitated in the form of zinc hydroxide compounds. The precipitate is washed, Drying to obtain treated zinc powder, obtaining lime, pouring it into the inside of a grinder for preliminary grinding, obtaining coarse lime powder passing through a 200-mesh sieve, and finally using a jet milling method for further processing to obtain lime powder. The processing method of ceramic particles is as follows: firstly, the particle size of the ceramic particles is screened to pass through a 50-mesh sieve, and then the ceramic particles are fully washed with deionized water to remove impurities and contaminants on the surface of the ceramic particles, and then high-temperature sterilization is performed to ensure that the ceramic particles are sterile, and finally surface treatment is performed to introduce bioactive molecules on the surface of the ceramic particles through a chemical modification method to obtain ceramic particles;

[0038] S2: Obtain corn flour, soybean flour, beef, metal powder, lime powder, ceramic particles, quartz sand and water, control the stirring speed at 1580r / min, control the stirring time at 12 minutes, mix evenly, add to a pressure cooker and boil, then naturally cool to 30°C, pour into a fermentation tank, and add yeast for fermentation. After the fermentation is completed, sterilize to obtain a culture solution. During the fermentation process, continuously stir the raw materials in the fermentation tank for 17 hours;

[0039] S3: Pour the culture medium into a sterile container, then put the Ganoderma lucidum to be cultivated, then put the sterile container into an automated temperature control box, use the automated temperature control system to start cultivating the Ganoderma lucidum, and cultivate it at a temperature of 28°C to 33°C.

[0040] S4: After 3 days of cultivation, the inside of the sterile container was stirred twice a day. After 11 days of cultivation, stirring was stopped and cultivation was continued for 4 months to obtain Ganoderma lucidum. After taking out the Ganoderma lucidum, the residual material in the sterile container was filtered to obtain Ganoderma lucidum culture solution and Ganoderma lucidum culture residue.

[0041] Reference Figure 1 , Embodiment 4

[0042] This embodiment proposes a method for cultivating Ganoderma lucidum using an automated temperature control system, comprising the following steps:

[0043] S1: Obtain meat ganoderma lucidum, culture solution and automatic temperature control box, the weight proportion of the culture solution includes 4% corn flour, 2% soybean flour, 0.5% beef, 6ppm metal powder, 0.4% lime powder, 3% ceramic particles, 2% quartz sand and the balance water, the metal powder includes treated iron powder and treated zinc powder, the weight ratio of the treated iron powder to the treated zinc powder is 3:1, the processing method of the iron powder is as follows: firstly, the iron powder to be treated is added into the grinding equipment for grinding to obtain the iron powder with a mesh size of 200, then the iron powder is added into ethanol, the ultrasonic wave is started to clean the iron powder, the iron powder is taken out after 5 minutes, and the iron powder is Pour in distilled water and start ultrasonic treatment again to remove impurities and oil on the surface of the iron powder to ensure the cleanliness of the iron powder surface. Then screen the iron powder to remove particles that are too large or too small, and select iron powder with a relatively uniform particle size to better control the amount added and the uniformity of the reaction. In this process, you can use 200-mesh and 250-mesh sieves for screening respectively, and obtain iron powder that passes the 200-mesh sieve but not the 250-mesh sieve. Then place the cleaned and screened iron powder in the air for a period of time to properly oxidize it so that a stable iron oxide layer is formed on its surface to reduce the activity of the iron powder. Finally, analyze the composition of the treated iron powder to confirm Ensure that no other harmful impurities are introduced, and finally obtain the treated iron powder. The treatment method of zinc powder: First, it is necessary to remove impurities in the zinc powder. Use a 200-mesh sieve to screen the zinc powder to remove larger particles of impurities and agglomerates. Wash the zinc powder three times with deionized water. During the washing process, remove the cleaning liquid by filtering to remove dust and other water-soluble impurities that may be attached to the surface of the zinc powder. Then, the zinc powder needs to be converted into a suitable form. Use dilute hydrochloric acid to dissolve the zinc powder to form a zinc ion solution. By adjusting the pH value and other methods, the zinc ions are precipitated in the form of zinc hydroxide compounds. The precipitate is washed, Drying to obtain treated zinc powder, obtaining lime, pouring it into the inside of a grinder for preliminary grinding, obtaining coarse lime powder passing through a 200-mesh sieve, and finally using a jet milling method for further processing to obtain lime powder. The processing method of ceramic particles is as follows: firstly, the particle size of the ceramic particles is screened to pass through a 50-mesh sieve, and then the ceramic particles are fully washed with deionized water to remove impurities and contaminants on the surface of the ceramic particles, and then high-temperature sterilization is performed to ensure that the ceramic particles are sterile, and finally surface treatment is performed to introduce bioactive molecules on the surface of the ceramic particles through a chemical modification method to obtain ceramic particles;

[0044] S2: Obtain corn flour, soybean flour, beef, metal powder, lime powder, ceramic particles, quartz sand and water, control the stirring speed at 1580r / min, control the stirring time at 12 minutes, mix evenly, add to a pressure cooker and boil, then naturally cool to 30°C, pour into a fermentation tank, and add yeast for fermentation. After the fermentation is completed, sterilize to obtain a culture solution. During the fermentation process, continuously stir the raw materials in the fermentation tank for 17 hours;

[0045] S3: Pour the culture medium into a sterile container, then put the Ganoderma lucidum to be cultivated, then put the sterile container into an automated temperature control box, use the automated temperature control system to start cultivating the Ganoderma lucidum, and cultivate it at a temperature of 28°C to 33°C.

[0046] S4: After 3 days of cultivation, the inside of the sterile container was stirred twice a day. After 11 days of cultivation, stirring was stopped and cultivation was continued for 4 months to obtain Ganoderma lucidum. After taking out the Ganoderma lucidum, the residual material in the sterile container was filtered to obtain Ganoderma lucidum culture solution and Ganoderma lucidum culture residue.

[0047] Reference Figure 1 Embodiment 5

[0048] This embodiment proposes a method for cultivating Ganoderma lucidum using an automated temperature control system, comprising the following steps:

[0049] S1: Obtain meat ganoderma lucidum, culture solution and automatic temperature control box, the weight proportion of the culture solution includes 2% corn flour, 1% soybean flour, 0.3% beef, 5ppm metal powder, 0.2% lime powder, 1% ceramic particles, 2% quartz sand and the balance water, the metal powder includes treated iron powder and treated zinc powder, the weight ratio of the treated iron powder to the treated zinc powder is 3:1, the processing method of iron powder is as follows: firstly, the iron powder to be treated is added into the grinding equipment for grinding to obtain the iron powder with a mesh size of 200, then the iron powder is added into ethanol, the ultrasonic wave is started to clean the iron powder, the iron powder is taken out after 5 minutes, and the iron powder is Pour in distilled water and start ultrasonic treatment again to remove impurities and oil on the surface of the iron powder to ensure the cleanliness of the iron powder surface. Then screen the iron powder to remove particles that are too large or too small, and select iron powder with a relatively uniform particle size to better control the amount added and the uniformity of the reaction. In this process, you can use 200-mesh and 250-mesh sieves for screening respectively, and obtain iron powder that passes the 200-mesh sieve but not the 250-mesh sieve. Then place the cleaned and screened iron powder in the air for a period of time to properly oxidize it so that a stable iron oxide layer is formed on its surface to reduce the activity of the iron powder. Finally, analyze the composition of the treated iron powder to confirm Ensure that no other harmful impurities are introduced, and finally obtain the treated iron powder. The treatment method of zinc powder: First, it is necessary to remove impurities in the zinc powder. Use a 200-mesh sieve to screen the zinc powder to remove larger particles of impurities and agglomerates. Wash the zinc powder three times with deionized water. During the washing process, remove the cleaning liquid by filtering to remove dust and other water-soluble impurities that may be attached to the surface of the zinc powder. Then, the zinc powder needs to be converted into a suitable form. Use dilute hydrochloric acid to dissolve the zinc powder to form a zinc ion solution. By adjusting the pH value and other methods, the zinc ions are precipitated in the form of zinc hydroxide compounds. The precipitate is washed, Drying to obtain treated zinc powder, obtaining lime, pouring it into the inside of a grinder for preliminary grinding, obtaining coarse lime powder passing through a 200-mesh sieve, and finally using a jet milling method for further processing to obtain lime powder. The processing method of ceramic particles is as follows: firstly, the particle size of the ceramic particles is screened to pass through a 50-mesh sieve, and then the ceramic particles are fully washed with deionized water to remove impurities and contaminants on the surface of the ceramic particles, and then high-temperature sterilization is performed to ensure that the ceramic particles are sterile, and finally surface treatment is performed to introduce bioactive molecules on the surface of the ceramic particles through a chemical modification method to obtain ceramic particles;

[0050] S2: Obtain corn flour, soybean flour, beef, metal powder, lime powder, ceramic particles, quartz sand and water, control the stirring speed at 1580r / min, control the stirring time at 12 minutes, mix evenly, add to a pressure cooker and boil, then naturally cool to 30°C, pour into a fermentation tank, and add yeast for fermentation. After the fermentation is completed, sterilize to obtain a culture solution. During the fermentation process, continuously stir the raw materials in the fermentation tank for 17 hours;

[0051] S3: Pour the culture medium into a sterile container, then put the Ganoderma lucidum to be cultivated, then put the sterile container into an automated temperature control box, use the automated temperature control system to start cultivating the Ganoderma lucidum, and cultivate it at a temperature of 28°C to 33°C.

[0052] S4: After 3 days of cultivation, the inside of the sterile container was stirred twice a day. After 11 days of cultivation, stirring was stopped and cultivation was continued for 4 months to obtain Ganoderma lucidum. After taking out the Ganoderma lucidum, the residual material in the sterile container was filtered to obtain Ganoderma lucidum culture solution and Ganoderma lucidum culture residue.

[0053] Comparing conventional meat ganoderma with the meat ganoderma obtained in Examples 1 to 5, the meat ganoderma obtained in Examples 1 to 5 are as follows:

[0054]

[0055] It can be seen from the above table that the Ganoderma lucidum prepared by the present invention has a high purity and no pesticides and heavy metal residues.

[0056] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A method for cultivating Ganoderma lucidum using an automated temperature control system, characterized in that: The following steps are involved: S1: Obtain meat ganoderma, culture solution and automatic temperature control box, wherein the culture solution comprises by weight 1% to 5% corn flour, 1% to 3% soybean flour, 0.2% to 1.0% beef, 5ppm to 10ppm metal powder, 0.2% to 1.0% lime powder, 1% to 5% ceramic particles, 1% to 3% quartz sand and the balance water; S2: Obtain corn flour, soybean flour, beef, metal powder, lime powder, ceramic particles, quartz sand and water, mix them evenly, add them into a pressure cooker to boil, then naturally cool them to 30°C, pour them into a fermentation tank, add yeast for fermentation, and after the fermentation is completed, sterilize them to obtain a culture solution; S3: Pour the culture medium into a sterile container, then put the Ganoderma lucidum to be cultivated, then put the sterile container into an automated temperature control box, and start cultivating Ganoderma lucidum using the automated temperature control system. S4: After 3 days of cultivation, the inside of the sterile container was stirred twice a day. After 8 to 12 days of cultivation, stirring was stopped and cultivation was continued for 3 to 5 months to obtain Ganoderma lucidum.

2. The method for cultivating Ganoderma lucidum using an automated temperature control system according to claim 1, characterized in that: In S1, the metal powder includes treated iron powder and treated zinc powder, and the weight ratio of the treated iron powder to the treated zinc powder is 3:

1.

3. The method for cultivating Ganoderma lucidum using an automated temperature control system according to claim 2, characterized in that: The method for processing the iron powder comprises the following steps: firstly, adding the iron powder to be processed into a grinding device for grinding to obtain iron powder that passes 200 meshes, then adding the iron powder into ethanol, starting ultrasonic wave to clean the iron powder, taking out the iron powder after 5 minutes, pouring the iron powder into distilled water, starting ultrasonic wave again for processing, removing impurities and oil stains on the surface of the iron powder, ensuring the cleanliness of the surface of the iron powder, then screening the iron powder, removing the part with oversized or undersized particles, and selecting iron powder with relatively uniform particle size, so as to better control the addition amount and the uniformity of the reaction. In this process, 200 mesh and 250 mesh sieves can be used for screening respectively to obtain iron powder that passes 200 mesh sieve but not 250 mesh sieve, then placing the cleaned and screened iron powder in the air for proper oxidation for a period of time to form a stable iron oxide layer on its surface to reduce the activity of the iron powder, and finally performing component analysis on the treated iron powder to ensure that no other harmful impurities are introduced, and finally obtaining the treated iron powder.

4. The method for cultivating Ganoderma lucidum using an automated temperature control system according to claim 2, characterized in that: The method for treating zinc powder comprises the following steps: first, impurities in the zinc powder need to be removed, the zinc powder is screened with a 200-mesh screen to remove larger particles of impurities and agglomerates, the zinc powder is washed three times with deionized water, during the washing process, the washing liquid is removed by filtering to remove dust and other water-soluble impurities that may be attached to the surface of the zinc powder, then the zinc powder needs to be converted into a suitable form, the zinc powder is dissolved with dilute hydrochloric acid to form a zinc ion solution, the zinc ions are precipitated in the form of zinc hydroxide compounds by adjusting the pH value and the like, and the precipitate is washed and dried to obtain the treated zinc powder.

5. The method for cultivating Ganoderma lucidum using an automated temperature control system according to claim 1, characterized in that: In S1, the preparation method of lime powder is as follows: lime is obtained, poured into a grinder for preliminary grinding, coarse lime powder is obtained after passing through a 200-mesh sieve, and finally the lime powder is processed again by a jet milling method to obtain lime powder.

6. The method for cultivating Ganoderma lucidum using an automated temperature control system according to claim 1, characterized in that: In S1, the method for processing ceramic particles is as follows: first, the ceramic particles are screened by particle size until they pass through a 50-mesh sieve, then the ceramic particles are thoroughly cleaned with deionized water to remove impurities and contaminants on the surface of the ceramic particles, then high-temperature sterilization is performed to ensure that the ceramic particles are sterile, and finally surface treatment is performed to introduce bioactive molecules on the surface of the ceramic particles through a chemical modification method, thereby obtaining ceramic particles.

7. The method for cultivating Ganoderma lucidum using an automated temperature control system according to claim 1, characterized in that: In S2, the stirring speed is controlled at 120 r / min to 180 r / min, and the stirring time is controlled at 10 minutes to 15 minutes.

8. The method for cultivating Ganoderma lucidum using an automated temperature control system according to claim 1, characterized in that: In S2, during the fermentation process, the raw materials in the fermentation tank are continuously stirred for 12 to 24 hours.

9. The method for cultivating Ganoderma lucidum using an automated temperature control system according to claim 1, characterized in that: In S3, the incubation is carried out at a temperature of 28°C to 33°C.

10. The method for cultivating Ganoderma lucidum using an automated temperature control system according to claim 1, characterized in that: In S4, after taking out the Ganoderma lucidum, the residual substances in the sterile container are filtered to obtain the Ganoderma lucidum culture solution and the Ganoderma lucidum culture residue.

Images