Nutrient solution containing chlorella as well as preparation method and application thereof

Abstract

The invention relates to the technical field of algae culture, in particular to a nutrient solution containing chlorella as well as a preparation method and application of the nutrient solution. Comprising a chlorella culture solution, nutrient components and a crushed grain hydrolysate, the chlorella culture solution is obtained through three-stage culture, and the nutrient components comprise a large amount of components and a small amount of components; the crushed grain hydrolysate is any one or more of crushed rice, crushed corn, crushed wheat and crushed oat. And proportionally adding nutritional components and the crushed grain hydrolysate into the culture solution containing chlorella subjected to three-stage culture, and mixing to obtain the nutrient solution containing chlorella. The nutrient solution containing the chlorella not only can provide required nutrients for the growth of forage grass, but also can assist the growth of the forage grass and improve the grass yield and the nutritional ingredients of the forage grass. The preparation process of the nutrient solution containing chlorella is simple in design, the required raw materials are cheap and easy to obtain, the cost is controlled within a reasonable range, and large-scale commercial production can be realized.

Description

Technical Field

[0001] The present application relates to the technical field of algae cultivation, and in particular to a nutrient solution containing Chlorella vulgaris and a preparation method and application thereof. Background Art

[0002] Hydroponic forage is a soilless cultivation technology that does not use soil to grow crops. Its principle is to directly sprinkle water on forage seeds to make the seeds grow rapidly under reasonable temperature and humidity control, avoiding the occupation of a large amount of arable land or forage land for planting forage. Its characteristics are low energy consumption, low water consumption, fast grass production speed, easy operation and easy maintenance. However, hydroponic forage needs to add a lot of nutrients during the cultivation process, which increases the cultivation cost. Therefore, a new cultivation technology has been developed, that is, the technology of cultivating algae and forage together. Forage hydroponic (microalgae) cultivation technology is a new technology that grafts microalgae on forage hydroponic technology, that is, replaces clean water with microalgae suspension. It grows faster and produces more grass than water forage. Hydroponic (microalgae) forage technology can effectively overcome the defects and disadvantages of natural conditions in some areas and produce high-quality forage. It has important practical significance for changing traditional animal husbandry methods, realizing innovation in animal husbandry technology, increasing the proportion of grassland animal husbandry in the agricultural industrial structure in our region, saving arable land and water in the project area, and protecting the grassland ecological environment in the project area.

[0003] Chlorella is a ubiquitous single-cell green algae, classified as Chlorophyta → Chlorophyceae → Chlorococcales → Oocystaceae → Chlorella. It is the first artificially cultivated microalgae. Chlorella is the earliest eukaryotic organism on Earth that uses light energy and carbon dioxide to maintain autotrophic life and has a primitive photosynthesis system. It is a cell that performs photosynthesis without oxygen under anaerobic conditions. It is a type of algae that uses light as an energy source and can use organic matter, sulfide, ammonia, etc. in nature as hydrogen and carbon sources for photosynthesis under anaerobic light or aerobic dark conditions. Chlorella cells have chloroplasts like plants and can perform oxygenic photosynthesis. There are other different pigments in the chloroplasts in Chlorella cells, including pigments such as carotene and lutein, which give Chlorella cells more colors and stronger photosynthetic advantages. Another function of Chlorella in utilizing photosynthesis is to use adenosine triphosphate (ATP) produced under strong sunlight to promote the combination of phosphoenolpyruvate (PEP) and CO2, thereby increasing the photosynthetic rate under strong light and high temperature. In drought, the stomatal aperture can be partially shrunk to reduce transpiration loss, while the degree of photosynthetic rate reduction is relatively small, thereby increasing the utilization rate of water in plants. In addition, the adsorption dynamics of Chlorella photosynthetic nutrient cells show that Chlorella can basically reach equilibrium in the adsorption of various metal ions within 30 minutes at the fastest. In recent years, with the development of algae biotechnology, Chlorella has not only become a good experimental biological model in the research of "space algae", photosynthesis mechanism, transmembrane transport mechanism, etc., but also has been widely used in environmental protection, health food, natural product carotenoids, bait and feed additives. In developed countries such as the United States and Japan, especially in Japan, it has been used as an excellent food and animal feed additive for more than 40 years. China's research on microalgae began in the 1950s. By the 1980s, people had learned that microalgae were a source of high-quality nutrients, and health products, foods and additives made from algae continued to emerge. Studies have shown that Chlorella is rich in a variety of nutrients and bioactive substances required for animal growth (including active growth factor (CGF), polysaccharides, biotin, and four major active ingredients such as chlorophyll and folic acid), has unique biological effects, and has obvious effects in animal feed. At present, Chlorella powder and its suspension have been approved by my country's Ministry of Agriculture and Rural Affairs as new feed additives, but there are few reports on the use of Chlorella culture solution in hydroponic forage.

[0004] In view of this, the present application provides a nutrient solution containing Chlorella and a preparation method and application thereof, and uses the Chlorella culture solution as a nutrient solution for hydroponic forage to achieve synergistic symbiosis between Chlorella and forage and obtain better economic and social benefits. Summary of the invention

[0005] In view of the fact that there are few applications of Chlorella culture solution in hydroponic forage in the prior art, the present application provides a nutrient solution containing Chlorella and a preparation method and application thereof.

[0006] The technical solution of this application:

[0007] In a first aspect, the present application provides a nutrient solution containing Chlorella, comprising Chlorella culture solution, nutrient components and cereal fragments hydrolyzate, wherein the ratio of the Chlorella culture solution, nutrient components and cereal fragments hydrolyzate is 1L: (0.5-2)g: (10-15)g; the Chlorella culture solution is obtained through three-stage culture, the nutrient components include macro-components and trace components; the cereal fragments hydrolyzate is any one or more of broken rice, broken corn, broken wheat and broken oats.

[0008] Preferably, the ratio of the Chlorella culture solution, the nutrient components and the grain crushed material hydrolyzate is 1 L: (100-300) mL: (0.5-2) g.

[0009] Preferably, the steps of the three-stage culture are as follows:

[0010] Step S1. Primary culture

[0011] After the original Chlorella species is activated, it is transferred to a primary container for cultivation, with a light intensity of 7000-10000 Lux and a ventilation flow rate of 2-3 L / min. The primary culture is obtained after culturing for 7-10 days.

[0012] Step S2. Secondary culture

[0013] The primary culture is transferred to a secondary container for cultivation at a light intensity of 7000 to 10000 Lux and a ventilation flow rate of 2.5 to 3.5 L / min, and the secondary culture is obtained after cultivation for 8 to 12 days;

[0014] Step S3. Tertiary culture

[0015] The secondary culture is transferred to a tertiary container for culture, the light intensity is 7000-10000 Lux, the ventilation flow rate is 2.5-3.5 L / min, and the culture is carried out for 18-25 days.

[0016] Preferably, the volume ratio of the primary container, the secondary container and the tertiary container is 1:(50-55):(175-185), and the culture medium for the primary culture, the secondary culture and the tertiary culture is BG-11 culture medium.

[0017] Preferably, the preparation method of the cereal crushed material hydrolyzate is as follows:

[0018] The grain powder is mixed with distilled water and gelatinized, then cooled to 60°C, 1,4-α-D-glucan glucanohydrolase is added for hydrolysis, and finally the 1,4-α-D-glucan glucanohydrolase is extinguished in a boiling water bath, and the supernatant is obtained by centrifugation; α-1,4-glucose hydrolase is added to the supernatant, hydrolyzed at 60°C for 4h to 5h, and then the α-1,4-glucose hydrolase is extinguished in a boiling water bath, and the supernatant is obtained by cooling to room temperature to obtain the grain powder hydrolyzate.

[0019] Preferably, the mass ratio of the cereal powder, distilled water, 1,4-α-D-glucan glucanohydrolase and α-1,4-glucanohydrolase is (40-45):(450-500):1:(3-5); the concentration of the 1,4-α-D-glucan glucanohydrolase is 3700 U / g, and the concentration of the α-1,4-glucanohydrolase is 105 U / g.

[0020] Preferably, the bulk components are urea, ammonium dihydrogen phosphate, potassium sulfate, ferrous sulfate, magnesium sulfate, and boric acid;

[0021] The trace components are sodium selenite, zinc sulfate, manganese sulfate, copper sulfate, ammonium molybdate, sodium sulfate, calcium dofenac, amino fresh fat DA-6, sodium naphthylacetate, EDTA and lysine.

[0022] Preferably, the addition amounts of the major components and trace components are, by mass percentage, 25-30% urea, 15-16% diammonium phosphate, 8-12% potassium sulfate, 8-12% ferrous sulfate, 6-10% magnesium sulfate, 6-8% boric acid; 3-4.5% sodium selenite, 3-4.5% zinc sulfate, 4-5% manganese sulfate, 4-6% copper sulfate, 0.1-0.2% ammonium molybdate, 0.5-1.5% sodium sulfate, 0.5-1.5% calcium dofenac, 0.2-0.6% DA-6, 0.2-0.6% sodium naphthylacetate, 2-4% EDTA and 0.5-1.5% lysine.

[0023] In a second aspect, the present application provides a method for preparing a nutrient solution containing Chlorella, the process is as follows:

[0024] Nutrient components and grain crushed material hydrolyzate are added to the culture solution containing Chlorella after the third-stage culture in proportion to obtain a nutrient solution containing Chlorella.

[0025] In a third aspect, the present application provides an application of a nutrient solution containing Chlorella for the cultivation of hydroponic forage.

[0026] Beneficial effects of this application:

[0027] (1) In the nutrient solution containing Chlorella in the present application, the chlorophyll and protein complex of Chlorella can help the forage grass repair damaged cells and inhibit common plant diseases; Chlorella growth factor (CGF) can activate plant immune cells, induce interferon, stimulate plant immunity, and enhance plant disaster resistance; the acidic polysaccharides in the cells of Chlorella can absorb and degrade heavy metals and pesticide residues in water. The nutrient solution containing Chlorella can not only provide the nutrients needed for the growth of forage grass, but also help the growth of forage grass, increase the grass yield and its nutritional content.

[0028] (2) The chlorella of the present application undergoes three-stage culture, which significantly improves the purity and activity of the chlorella. Each stage of culture can remove impurities, promote the growth and reproduction of chlorella, and ensure that the final chlorella is of high quality and activity. The three-stage culture can also enhance the adaptability and stress resistance of chlorella: during the three-stage culture process, chlorella will experience different environmental conditions, such as changes in temperature, light, nutrient concentration, etc. This helps to enhance the adaptability and stress resistance of chlorella, enabling it to better adapt to various growth environments and improve its survival ability and competitiveness.

[0029] (3) The preparation process of the nutrient solution containing Chlorella in the present application is simple in design, the required raw materials are cheap and easy to obtain, the cost is controlled within a reasonable range, and large-scale commercial production is possible.

[0030] (4) The present application also adds hydrolyzed grain fragments into the nutrient solution. When the forage grass affects the photosynthesis of Chlorella during its growth, Chlorella can use the organic carbon source in the hydrolyzed grain fragments to carry out heterogeneous metabolism. The grain fragments can also be fully utilized, and the harvested Chlorella can be further processed. DETAILED DESCRIPTION

[0031] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the specific implementation mode, structure, characteristics and effects of the present invention are described in detail below in combination with the preferred embodiments. It should be noted that the chlorella used in this application is Chlorella vulgaris.

[0032] Example 1

[0033] This embodiment provides a method for preparing a culture solution containing Chlorella, which is as follows:

[0034] Step 1: Cultivation of Chlorella

[0035] Step S1. Activate the Chlorella vulgaris and transfer it into a culture container with a volume of 1 L and containing 2 / 3 volume of BG-11. A vent is provided at the bottom of the container, and gas is introduced into the culture container through the vent. The gas flow rate is 2.5 L / min. The gas is sterilized with a cotton ball before entering the culture container. The culture is carried out for 8 days under a light intensity of 8000 Lux and a culture temperature of 27° C. to obtain a primary culture;

[0036] Step S2. The primary culture was transferred into a 50 L culture container containing 2 / 3 volume of BG-11. A vent was provided at the bottom of the container. Gas was introduced into the culture container through the vent at a flow rate of 3 L / min. The culture was carried out for 10 days at a light intensity of 8000 Lux and a culture temperature of 27° C. to obtain a secondary culture.

[0037] Step S3. The secondary culture was transferred into a culture container with a volume of 185 L and containing 2 / 3 volume of BG-11. A vent was provided at the bottom of the container. Gas was introduced into the culture container through the vent at a gas flow rate of 3 L / min. The culture was cultured for 22 days at a light intensity of 80,000 Lux and a culture temperature of 27° C. to obtain a tertiary culture.

[0038] Step 2: Preparation of hydrolyzate of grain fragments

[0039] After removing impurities from the broken rice, crush it with a grinder and pass it through a 100-mesh sieve to obtain grain powder. Take 43g of grain powder and mix it with 500mL of distilled water, gelatinize it in a 100℃ water bath for 30min, and cool it to 60℃. Then add 1g of 1,4-α-D-glucan glucanohydrolase and enzymolyze it in a 60℃ water bath for 30min to liquefy the grain powder. Boil the liquefied grain powder for 15min to inactivate the 1,4-α-D-glucan glucanohydrolase, cool it to room temperature, centrifuge it at 4500r / min for 15min to obtain the supernatant, add 5g of α-1,4-glucanohydrolase to the supernatant, and then enzymolyze it in a 60℃ water bath for 4-5h to obtain the grain powder hydrolyzate. The hydrolyzate of the grain fragments was boiled for 10 minutes to inactivate the α-1,4-glucose hydrolase, cooled to room temperature, and then filtered with a 0.45 μm filter membrane to obtain the hydrolyzate of the grain fragments of the present application.

[0040] Step 3: Preparation method of culture solution containing Chlorella

[0041] 50L of the third-level culture, 700g of the hydrolyzed grain material and 50g of the nutrient components are mixed to obtain the culture solution containing Chlorella of the present application. The proportion of each substance in the nutrient components is 25% urea, 16% diammonium phosphate, 8% potassium sulfate, 12% ferrous sulfate, 6% magnesium sulfate, 8% boric acid; 3% sodium selenite, 4.5% zinc sulfate, 4% manganese sulfate, 6% copper sulfate, 0.12% ammonium molybdate, 1.5% sodium sulfate, 0.5% calcium dofenitol, 0.6% amino fresh fat DA-6, 0.2% sodium naphthylacetate, 3.1% EDTA and 1.5% lysine.

[0042] Example 2

[0043] This embodiment provides a method for preparing a culture solution containing Chlorella, which is as follows:

[0044] Step 1: Cultivation of Chlorella

[0045] Step S1. Activate the Chlorella vulgaris and transfer it into a culture container with a volume of 1 L and containing 2 / 3 volume of BG-11. A vent is provided at the bottom of the container, and gas is introduced into the culture container through the vent. The gas flow rate is 3 L / min. The gas is sterilized with a cotton ball before entering the culture container. The culture is carried out for 9 days under a light intensity of 7000 Lux and a culture temperature of 28° C. to obtain a primary culture;

[0046] Step S2. The primary culture was transferred into a 55 L culture container containing 2 / 3 volume of BG-11. A vent was provided at the bottom of the container. Gas was introduced into the culture container through the vent at a flow rate of 3.5 L / min. The culture was carried out for 12 days at a light intensity of 7000 Lux and a culture temperature of 28° C. to obtain a secondary culture.

[0047] Step S3. The secondary culture was transferred into a culture container with a volume of 175 L and containing 2 / 3 volume of BG-11. A vent was provided at the bottom of the container. Gas was introduced into the culture container through the vent at a flow rate of 3.5 L / min. The culture was carried out for 18 days under a light intensity of 7000 Lux and a culture temperature of 28° C. to obtain a tertiary culture.

[0048] Step 2: Preparation of hydrolyzate of grain fragments

[0049] After removing impurities from the crushed corn, crush it with a grinder and pass it through a 100-mesh sieve to obtain grain powder. Take 42g of grain powder and mix it with 500mL of distilled water, gelatinize it in a 100℃ water bath for 30min, and cool it to 60℃. Then add 1g of 1,4-α-D-glucan glucanohydrolase and enzymolyze it in a 60℃ water bath for 30min to liquefy the grain powder. Boil the liquefied grain powder for 15min to inactivate the 1,4-α-D-glucan glucanohydrolase, cool it to room temperature, centrifuge it at 4500r / min for 15min to obtain the supernatant, add 4g of α-1,4-glucanohydrolase to the supernatant, and then enzymolyze it in a 60℃ water bath for 4-5h to obtain the grain powder hydrolyzate. The hydrolyzate of the grain fragments was boiled for 10 minutes to inactivate the α-1,4-glucose hydrolase, cooled to room temperature, and then filtered with a 0.45 μm filter membrane to obtain the hydrolyzate of the grain fragments of the present application.

[0050] Step 3: Preparation method of culture solution containing Chlorella

[0051] 50L of the tertiary culture, 500g of the hydrolyzed grain material and 100g of the nutrient components are mixed to obtain the culture solution containing Chlorella of the present application. The proportion of each substance in the nutrient components is 30% urea, 15% diammonium phosphate, 10% potassium sulfate, 10% ferrous sulfate, 7% magnesium sulfate, 6.5% boric acid; 3.2% sodium selenite, 3.1% zinc sulfate, 4.3% manganese sulfate, 4.5% copper sulfate, 0.1% ammonium molybdate, 0.6% sodium sulfate, 0.8% calcium dofenitol, 0.3% amino fresh fat DA-6, 0.5% sodium naphthylacetate, 2.8% EDTA and 1.3% lysine.

[0052] Example 3

[0053] This embodiment provides a method for preparing a culture solution containing Chlorella, which is as follows:

[0054] Step 1: Cultivation of Chlorella

[0055] Step S1. Activate the Chlorella vulgaris and transfer it into a culture container with a volume of 1 L and containing 2 / 3 volume of BG-11. A vent is provided at the bottom of the container, and gas is introduced into the culture container through the vent. The gas flow rate is 2 L / min. The gas is sterilized with a cotton ball before entering the culture container. The culture is carried out for 7 days under a light intensity of 10000 Lux and a culture temperature of 26° C. to obtain a primary culture;

[0056] Step S2. The primary culture was transferred into a 50 L culture container containing 2 / 3 volume of BG-11. A vent was provided at the bottom of the container. Gas was introduced into the culture container through the vent at a flow rate of 2.5 L / min. The culture was carried out for 8 days at a light intensity of 10,000 Lux and a culture temperature of 26° C. to obtain a secondary culture.

[0057] Step S3. The secondary culture was transferred into a culture container with a volume of 180 L and containing 2 / 3 volume of BG-11. A vent was provided at the bottom of the container. Gas was introduced into the culture container through the vent at a flow rate of 2.5 L / min. The culture was cultured for 25 days at a light intensity of 10,000 Lux and a culture temperature of 26° C. to obtain a tertiary culture.

[0058] Step 2: Preparation of hydrolyzate of grain fragments

[0059] After removing impurities from the crushed wheat, crush it with a grinder and pass it through a 100-mesh sieve to obtain grain powder. Take 45g of grain powder and mix it with 450mL of distilled water, gelatinize it in a 100℃ water bath for 30min, and cool it to 60℃. Then add 1g of 1,4-α-D-glucan glucanohydrolase and enzymolyze it in a 60℃ water bath for 30min to liquefy the grain powder. Boil the liquefied grain powder for 15min to inactivate the 1,4-α-D-glucan glucanohydrolase, cool it to room temperature, centrifuge it at 4500r / min for 15min to obtain the supernatant, add 4g of α-1,4-glucanohydrolase to the supernatant, and then enzymolyze it in a 60℃ water bath for 4-5h to obtain the grain powder hydrolyzate. The hydrolyzate of the grain fragments was boiled for 10 minutes to inactivate the α-1,4-glucose hydrolase, cooled to room temperature, and then filtered with a 0.45 μm filter membrane to obtain the hydrolyzate of the grain fragments of the present application.

[0060] Step 3: Preparation method of culture solution containing Chlorella

[0061] 50L of the third-level culture, 750g of the hydrolyzed grain material and 25g of the nutrient components are mixed to obtain the culture solution containing Chlorella of the present application. The proportion of each substance in the nutrient components is 28% urea, 15.5% diammonium phosphate, 9% potassium sulfate, 9% ferrous sulfate, 8% magnesium sulfate, 7% boric acid; 3.5% sodium selenite, 3.5% zinc sulfate, 4.5% manganese sulfate, 5% copper sulfate, 0.2% ammonium molybdate, 1% sodium sulfate, 1% calcium dofenitol, 0.5% amino fresh fat DA-6, 0.3% sodium naphthylacetate, 3% EDTA and 1% lysine.

[0062] Example 4

[0063] This embodiment provides a method for preparing a culture solution containing Chlorella, which is as follows:

[0064] Step 1: Cultivation of Chlorella

[0065] Step S1. Activate the Chlorella vulgaris and transfer it into a culture container with a volume of 1 L and containing 2 / 3 volume of BG-11. A vent is provided at the bottom of the container, and gas is introduced into the culture container through the vent. The gas flow rate is 3 L / min. The gas is sterilized with a cotton ball before entering the culture container. The culture is carried out for 9 days under a light intensity of 9000 Lux and a culture temperature of 27° C. to obtain a primary culture;

[0066] Step S2. The primary culture was transferred into a 55 L culture container containing 2 / 3 volume of BG-11. A vent was provided at the bottom of the container. Gas was introduced into the culture container through the vent at a gas flow rate of 3 L / min. The culture was carried out for 11 days under a light intensity of 9000 Lux and a culture temperature of 27° C. to obtain a secondary culture.

[0067] Step S3. The secondary culture was transferred into a culture container with a volume of 180 L and containing 2 / 3 volume of BG-11. A vent was provided at the bottom of the container, and gas was introduced into the culture container through the vent at a flow rate of 3 L / min. The culture was cultured for 20 days at a light intensity of 90,000 Lux and a culture temperature of 27° C. to obtain a tertiary culture;

[0068] Step 2: Preparation of hydrolyzate of grain fragments

[0069] Mix broken rice and broken oats in a mass ratio of 1:1, remove impurities and crush with a grinder, pass through a 100-mesh sieve to obtain grain powder. Take 40g of grain powder and mix it with 500mL of distilled water, gelatinize it in a 100℃ water bath for 30min, and cool it to 60℃. Then add 1g of 1,4-α-D-glucan glucanohydrolase, and enzymolyze it in a 60℃ water bath for 30min to liquefy the grain powder. Boil the liquefied grain powder for 15min to inactivate the 1,4-α-D-glucan glucanohydrolase, cool it to room temperature, centrifuge it at 4500r / min for 15min, and obtain the supernatant. Add 3g of α-1,4-glucanohydrolase to the supernatant, and then enzymolyze it in a 60℃ water bath for 4-5h to obtain the grain powder hydrolyzate. The hydrolyzate of the grain fragments was boiled for 10 minutes to inactivate the α-1,4-glucose hydrolase, cooled to room temperature, and then filtered with a 0.45 μm filter membrane to obtain the hydrolyzate of the grain fragments of the present application.

[0070] Step 3: Preparation method of culture solution containing Chlorella

[0071] 50L of the tertiary culture, 600g of the hydrolyzed grain material and 80g of the nutrient components are mixed to obtain the culture solution containing Chlorella of the present application. The proportion of each substance in the nutrient components is 26% urea, 15% diammonium phosphate, 11% potassium sulfate, 8% ferrous sulfate, 10% magnesium sulfate, 6% boric acid; 4.5% sodium selenite, 3% zinc sulfate, 5% manganese sulfate, 4% copper sulfate, 0.2% ammonium molybdate, 0.5% sodium sulfate, 1.5% calcium dofenitol, 0.2% amino fresh fat DA-6, 0.6% sodium naphthylacetate, 4% EDTA and 0.5% lysine.

[0072] Performance Testing

[0073] In order to verify the cultivation effect of the nutrient solution containing Chlorella prepared by the method of the present application on hydroponic forage, a planting experiment was carried out using barley grass, and the yield and nutritional components of the barley grass were tested.

[0074] The culture medium used in control group 1 was clear water, the culture medium used in control group 2 was not added with cereal crushed material hydrolyzate, the culture medium prepared in Example 1 was used in test group 2, the culture medium prepared in Example 2 was used in test group 3, and the culture medium prepared in Example 4 was used in test group 4. The nutritional components of barley grass are shown in Table 1, and the yield of barley grass is shown in Table 2, which is calculated based on the mass of fresh barley grass produced per kilogram of barley grass seeds.

[0075] Table 1

[0076] project Experimental Group 1 Experimental Group 2 Experimental Group 3 Experimental Group 4 Control group 1 Control group 2 Moisture / % 88.50 83.20 85.14 86.25 89.30 88.91 protein / % 31.90 32.80 30.97 31.96 28.80 29.86 calcium / % 0.18 0.19 0.23 0.20 0.17 0.18 magnesium / % 0.23 0.26 0.24 0.23 0.25 0.25 Potassium / % 2.26 2.29 2.24 2.30 2.12 2.19 nitrogen / % 4.88 4.96 4.87 4.91 4.25 4.51 sodium / % 0.12 0.11 0.13 0.12 0.12 0.11 phosphorus / % 0.98 0.99 0.96 0.95 0.91 0.93 Manganese / mg / kg 52.68 53.19 52.94 52.17 51.33 51.64 Zinc / mg / kg 62.65 63.12 62.75 62.94 57.09 60.45 Copper / mg / kg 26.76 27.34 28.15 26.98 28.13 27.31 Iron / mg / kg 215.32 215.79 216.32 217.36 217.66 216.12

[0077] Table 2

[0078]

[0079] Combining Examples 1 to 4 and Tables 1 to 2, it can be seen that the yield of barley grass cultured with the Chlorella-containing nutrient solution prepared in the present application is significantly higher than that of barley grass cultured in clear water (Control Group 1), especially the protein content in the nutrient components. It can also be found that the addition of grain crushed material hydrolyzate helps to increase the yield and nutrient content of barley grass. This may be because the amino acids, vitamins and minerals contained in the grain crushed material hydrolyzate may promote the root development and nutrient absorption of barley grass, which has a positive promoting effect on the growth and development of barley grass.

[0080] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not used to limit the present invention. Any technical personnel in this field can make some changes or modify the technical contents disclosed above into equivalent embodiments without departing from the scope of the technical solution of the present invention. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A nutrient solution containing Chlorella, characterized in that: The invention comprises chlorella culture solution, nutrient components and grain fragment hydrolysate, wherein the ratio of the chlorella culture solution, nutrient components and grain fragment hydrolysate is 1L: (0.5-2)g: (10-15)g; the chlorella culture solution is obtained through three-stage culture, the nutrient components comprise macro-components and trace components; the grain fragment hydrolysate is any one or more of broken rice, broken corn, broken wheat and broken oats.

2. The nutrient solution containing Chlorella according to claim 1, characterized in that: The ratio of the chlorella culture solution, the nutrient components and the grain crushed material hydrolyzate is 1L: (100-300)mL: (0.5-2)g.

3. The nutrient solution containing Chlorella according to claim 1, characterized in that: The steps of the three-stage culture are as follows: Step S1. Primary culture After the original Chlorella species is activated, it is transferred to a primary container for cultivation, with a light intensity of 7000-10000 Lux and a ventilation flow rate of 2-3 L / min. The primary culture is obtained after culturing for 7-10 days. Step S2. Secondary culture The primary culture is transferred to a secondary container for cultivation at a light intensity of 7000 to 10000 Lux and a ventilation flow rate of 2.5 to 3.5 L / min, and the secondary culture is obtained after cultivation for 8 to 12 days; Step S3. Tertiary culture The secondary culture is transferred to a tertiary container for culture, the light intensity is 7000-10000 Lux, the ventilation flow rate is 2.5-3.5 L / min, and the culture is carried out for 18-25 days.

4. The nutrient solution containing Chlorella according to claim 3, characterized in that: The volume ratio of the primary container, the secondary container and the tertiary container is 1:(50-55):(175-185), and the culture medium for the primary culture, the secondary culture and the tertiary culture is BG-11 culture medium.

5. The nutrient solution containing Chlorella according to claim 1, characterized in that: The preparation method of the cereal crushed material hydrolyzate is as follows: The grain powder is mixed with distilled water and gelatinized, then cooled to 60°C, 1,4-α-D-glucan glucanohydrolase is added for hydrolysis, and finally the 1,4-α-D-glucan glucanohydrolase is extinguished in a boiling water bath, and the supernatant is obtained by centrifugation; α-1,4-glucose hydrolase is added to the supernatant, hydrolyzed at 60°C for 4h to 5h, and then the α-1,4-glucose hydrolase is extinguished in a boiling water bath, and the supernatant is obtained by cooling to room temperature to obtain the grain powder hydrolyzate.

6. The nutrient solution containing Chlorella according to claim 5, characterized in that: Said The mass ratio of cereal powder, distilled water, 1,4-α-D-glucan glucanohydrolase and α-1,4-glucanohydrolase is (40-45):(450-500):1:(3-5); the concentration of the 1,4-α-D-glucan glucanohydrolase is 3700 U / g, and the concentration of the α-1,4-glucanohydrolase is 105 U / g.

7. The nutrient solution containing Chlorella according to claim 1, characterized in that: The bulk components are urea, ammonium dihydrogen phosphate, potassium sulfate, ferrous sulfate, magnesium sulfate, and boric acid; The trace components are sodium selenite, zinc sulfate, manganese sulfate, copper sulfate, ammonium molybdate, sodium sulfate, calcium dofenac, amino fresh fat DA-6, sodium naphthylacetate, EDTA and lysine.

8. The nutrient solution containing Chlorella according to claim 7, characterized in that: The addition amounts of the major components and trace components are calculated by mass percentage as follows: 25-30% urea, 15-16% diammonium dihydrogen phosphate, 8-12% potassium sulfate, 8-12% ferrous sulfate, 6-10% magnesium sulfate, 6-8% boric acid; 3-4.5% sodium selenite, 3-4.5% zinc sulfate, 4-5% manganese sulfate, 4-6% copper sulfate, 0.1-0.2% ammonium molybdate, 0.5-1.5% sodium sulfate, 0.5-1.5% calcium dofenac, 0.2-0.6% DA-6, 0.2-0.6% sodium naphthylacetate, 2-4% EDTA and 0.5-1.5% lysine.

9. The method for preparing a nutrient solution containing Chlorella according to any one of claims 1 to 8, characterized in that: The process is as follows: Nutrient components and grain crushed material hydrolyzate are added to the culture solution containing Chlorella after the third-stage culture in proportion to obtain a nutrient solution containing Chlorella.

10. The use of a nutrient solution containing Chlorella according to any one of claims 1 to 8, characterized in that: Used for cultivation of hydroponic forage.

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