Kit for rapidly detecting okadaic acid

Abstract

The invention relates to the technical field of aquatic product detection, and discloses a rapid okadaic acid detection kit which comprises a kit body, a kit cover is rotatably connected to an opening of the kit body, the kit body serves as a carrier, a sample barrel, a test tube and a dropper are arranged in the kit body, the sample barrel is used for sampling and processing, and the test tube is arranged in the sample barrel. The test tube is used for placing a treated sample, and the dropper is used for quantitatively taking out the sample in the test tube; a colorimetric card and a support plate which can be folded and stored are mounted on one side, close to the box body, of the box cover, the support plate is used for placing test paper, and the colorimetric card is used for color contrast. The sample barrel and the test tubes are placed in the box body, samples can be treated by the sampling barrel, then the samples and an extraction solution in the sample barrel are taken out by the dropper and added into the test tubes to obtain a sample solution, and finally test paper is placed on the support plate. After a sample solution is dropped on the test paper, the information of okadaic acid in aquatic products can be obtained through the colorimetric card, and on-site rapid detection is realized.

Description

Technical Field

[0001] The invention relates to the technical field of aquatic product detection, in particular to a rapid detection kit for okadaic acid. Background Art

[0002] Okadai acid (OA) is a diarrhetic shellfish toxin (DSP) mainly produced by certain marine phytoplankton (such as Prorocentrum spp. and Gymnodinium catenellae). This toxin is transmitted into shellfish (such as mussels, oysters, scallops, etc.) through the food chain. When humans ingest seafood containing OA, it can cause symptoms of acute gastroenteritis, including nausea, vomiting, diarrhea and abdominal pain, which can be life-threatening in severe cases.

[0003] Therefore, it is necessary to test okanamic acid in aquatic products. Food poisoning incidents or potential food safety risks can be avoided through testing. Therefore, it is crucial for food processing companies, catering service agencies, fishery management departments, etc. in the food supply chain to prevent harmful seafood from entering the market and protect the health of consumers by identifying and isolating contaminated food.

[0004] At present, the commonly used methods for detecting okadaic acid include liquid chromatography-mass spectrometry (LC-MS / MS), gas chromatography (GC) and enzyme-linked immunosorbent assay (ELISA). Although these methods are highly sensitive and accurate, they are complex to operate, require expensive instruments and equipment, are time-consuming, and require professional technicians to operate, thus limiting their application in rapid on-site detection. Summary of the invention

[0005] In view of the deficiencies in the prior art, the present invention provides a rapid detection kit for okadaic acid, which solves the problem in the prior art that okadaic acid detection is complicated in operation, requires expensive instruments and equipment, is time-consuming, and requires professional technicians to operate, so rapid detection cannot be performed on site.

[0006] To achieve the above object, the present invention is implemented by the following technical scheme: a rapid detection kit for okadaic acid, comprising:

[0007] A box body, the opening of which is rotatably connected to a box cover, the box body serving as a carrier, and a sample barrel, a test tube and a dropper are arranged inside the box body, the sample barrel is used for sampling and processing, the test tube is used for placing processed samples, and the dropper is used for quantitatively taking out samples from the test tube;

[0008] A colorimetric card and a foldable support plate are installed on one side of the box cover close to the box body. The support plate is used to place the test paper, and the colorimetric card is used for color comparison.

[0009] Preferably, a cover shell and a retaining ring are fixed at the edge of the box cover close to one side of the box body, a motor 1 is installed inside the cover shell, an insertion rod is fixed to the output end of the motor 1, a support cover is installed in the middle of the retaining ring, the bottom of the support cover is movably connected to the sample barrel, a rotating shaft is rotatably provided in the middle of the support cover, a crushing knife is installed on one side of the rotating shaft, and the other side of the rotating shaft is connected to the insertion rod, and the crushing knife is located in the sample barrel.

[0010] Preferably, a slot is provided at one end of the rotating shaft, the insertion rod is located in the slot, and the depth of the slot is greater than the sum of the length of the insertion rod and the thickness of the clamping ring, and one side of the support plate is fixedly connected to a limiting plate.

[0011] Preferably, ear blocks are fixed on opposite sides of the outer wall of the support cover, a through hole is formed on one opposite side of the inner part of the clamping ring, a groove is formed on the other opposite side of the inner part of the clamping ring, and the ear blocks are located in the groove after passing through the through hole.

[0012] Preferably, one side of the box cover is rotatably connected to a clamping rod, and the cross-section of the clamping rod is set to be T-shaped, which is used to limit the support plate after the support plate is folded and stored.

[0013] Preferably, motor 2 is fixed to one side of the inner bottom of the box body, a rotating plate is fixed to the output end of motor 2, a connecting rod is fixed to one side of the rotating plate, the outer wall of the connecting rod is rotatably connected to a swing arm, one end of the swing arm is rotatably connected to a bracket, the outer wall of the bracket is slidably connected to a support, the bottom of the support is fixedly connected to a leg, the leg is fixedly connected to the box body, and the test tube is located inside the bracket.

[0014] Preferably, a pad is fixedly connected to the bottom surface of the inner wall of the bracket, the bottom of the test tube is located inside the pad, and a rubber ring is fixedly connected to the top of the bracket, and the rubber ring is located on the outer wall of the test tube.

[0015] Preferably, a baffle is fixedly connected to the top of one side wall inside the box body, a sleeve is fixedly connected to the bottom surface of the baffle, the dropper is located in the sleeve after passing through the baffle, and the support is fixedly connected to the baffle.

[0016] Preferably, a storage box is fixedly connected to the middle of the bottom surface of the box body, a box cover is rotatably connected to the top of the storage box, and the top of the outer wall of the storage box is fixedly connected to the baffle.

[0017] Preferably, the other side wall inside the box body is fixedly connected to a base, a storage groove is opened on the top surface of the base, the retaining ring, support cover and sample barrel are all located in the storage groove, and a battery is installed on the inner bottom of the base, and the battery is connected to motor two and motor one.

[0018] Working principle: When in use, first open the box lid, then rotate the sample barrel to separate it from the support cover, then place the sample in the sample barrel, and finally tighten the sample barrel to the bottom of the support cover again. At this time, the driving motor 1 drives the insertion rod to rotate, and the insertion rod drives the rotating shaft to rotate. At this time, the rotating shaft can drive the crushing knife to crush the sample, and then remove the sample barrel and take out a dropper to take out the crushed sample and add it into the test tube, and then take out another test tube and add the extraction solution into the test tube. At this time, the driving motor 2 drives the rotating plate and the connecting rod to rotate. At this time, the connecting rod will drive the swing arm to swing, and the swing arm will pull the bracket to reciprocate inside the bracket. Therefore, the bracket can be used to drive the test tube to swing to speed up the fusion of the sample and the extraction solution, and then rotate the card rod and move the support plate to make it vertical to the box lid. After the sample and the extraction solution are fully mixed, the sample solution is obtained, and then another dropper is used to take the mixed sample solution out The sample is then placed on the support plate and the lid is opened to allow the ear block to be removed from the groove.

[0019] The present invention provides a rapid detection kit for okadaic acid. It has the following beneficial effects:

[0020] 1. The present invention places a sample barrel and a test tube in a box body, uses a sampling barrel to process the sample, then uses a dropper to take out the sample and the extraction solution in the sample barrel and add them into the test tube to obtain the sample solution, finally places the test paper on a support plate, drops the sample solution on the test paper, and then obtains the information of okara acid in aquatic products through a colorimetric card, thereby realizing on-site rapid detection.

[0021] 2. The present invention places a test tube in a bracket, drives a rotating plate and a connecting rod to rotate by driving a second motor, and drives a swing arm to swing by utilizing the connecting rod, thereby driving the bracket to reciprocate inside the support. At this time, the extraction solution and the sample in the test tube can be fully integrated, thereby being helpful to improve the accuracy of the final measured information of field soft sponge acid in aquatic products.

[0022] 3. The present invention tightens the sample barrel to the bottom of the support cover, and then drives the motor to rotate the insertion rod, the rotating shaft and the crushing knife, so that the sample can be crushed, which helps to improve the solubility and extraction efficiency of the target substance, thereby ensuring the accuracy and sensitivity of the test results.

[0023] 4. The present invention provides a through hole and a groove on the inner wall of the clamping ring. After the detection is completed, the support cover is extended to move the ear block out of the groove. The support cover is rotated to make the ear block coincide with the position of the through hole and then moved downward to separate it from the clamping ring, thereby cleaning the crushing knife and the sample barrel. After cleaning, the support cover is moved to make the ear block pass through the through hole, and finally the support rod is rotated to make the ear block enter the groove. The sample barrel can be separated from the support cover by rotating it, thereby facilitating the cleaning of the sample barrel and the crushing knife, thereby facilitating the accuracy of the detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention;

[0025] Figure 2 for Figure 1 A in the enlarged view;

[0026] Figure 3 This is a partial structural diagram of the sample barrel of the present invention;

[0027] Figure 4 for Figure 3 The enlarged view of point B in the figure;

[0028] Figure 5 It is a front cross-sectional view of the sleeve of the present invention;

[0029] Figure 6 for Figure 5 The enlarged view of point C in the figure;

[0030] Figure 7 It is a schematic diagram of the structure of the support part of the present invention;

[0031] Figure 8 This is a structural breakdown diagram of the support portion of the present invention;

[0032] Fig. 9 It is a right side cross-sectional view of the support of the present invention;

[0033] Fig.10 for Fig. 9 The enlarged view of D in the figure;

[0034] Fig.11 It is a right side cross-sectional view of the support plate of the present invention.

[0035] Among them, 1. box body; 2. box cover; 3. sample barrel; 4. test tube; 5. dropper; 6. support plate; 7. colorimetric card; 8. motor 1; 9. plug rod; 10. snap ring; 11. support cover; 12. rotating shaft; 13. crushing knife; 14. slot; 15. ear block; 16. through hole; 17. groove; 18. cover; 19. clamping rod; 20. motor 2; 21. rotating plate; 22. connecting rod; 23. swing arm; 24. bracket; 25. support; 26. support leg; 27. pad; 28. rubber ring; 29. ​​baffle; 30. sleeve; 31. base; 32. storage slot; 33. battery; 34. storage box; 35. box cover; 36. limit plate. DETAILED DESCRIPTION

[0036] The following will be combined with the drawings in the specification of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0037] Please see attached Figure 1 and Figure 5 The embodiment of the present invention provides a rapid detection kit for okadaic acid, comprising: a box body 1, a box cover 2 is rotatably connected to the opening of the box body 1, the box body 1 serves as a carrier, and a sample barrel 3, a test tube 4 and a dropper 5 are arranged inside the box body 1, the sample barrel 3 is used for sampling and processing, the test tube 4 is used for placing the processed sample, and the dropper 5 is used for quantitatively taking out the sample in the test tube 4; a colorimetric card 7 and a foldable support plate 6 are installed on the side of the box cover 2 close to the box body 1, the support plate 6 is used for placing the test paper, and the colorimetric card 7 is used for color comparison.

[0038] When in use, first open the box cover 2, then take out the sample barrel 3, cut the sample to be measured into pieces and put it into the sample barrel 3, or grind it and then put it into the sample barrel 3, so that its tissue fluid can seep out, then use the dropper 5 to titrate the obtained sample tissue fluid into the test tube 4, then take out another test tube 4 and titrate the extraction solution into the test tube 4, at this time the extraction solution will destroy the tissue structure or cell membrane in the sample, so that the cell fluid and the contents therein are released into the solution, then place the test paper on the support plate 6, and then take out a new dropper 5 to drop the solution on the test paper, at this time the okanamic acid in the solution will react with the test paper and produce a color change, and finally compare it with the colorimetric card 7 to determine the data of the okanamic acid in the sample.

[0039] Please see attached Figure 1 , Figure 3 and Figure 4 A cover shell 18 and a snap ring 10 are fixed at the edge of the box cover 2 close to the box body 1. A motor 8 is installed inside the cover shell 18. An insertion rod 9 is fixed to the output end of the motor 8. A support cover 11 is installed in the middle of the snap ring 10. The bottom of the support cover 11 is movably connected to the sample barrel 3. A rotating shaft 12 is rotatably provided in the middle of the support cover 11. A crushing knife 13 is installed on one side of the rotating shaft 12, and the other side of the rotating shaft 12 is connected to the insertion rod 9, and the crushing knife 13 is located in the sample barrel 3.

[0040] After the sample is added into the sample barrel 3, the motor 8 can be driven to drive the insertion rod 9 to rotate. After the insertion rod 9 rotates, it will drive the rotating shaft 12 and the crushing knife 13 to rotate, so that the sample can be crushed, thereby effectively refining its particles, thereby increasing its contact area with the extract, and further improving the infiltration effect of the internal tissue fluid, which is beneficial to improving the accuracy of the final test results.

[0041] Please see attached Figure 1 , Figure 3 and Figure 4 A slot 14 is provided at one end of the rotating shaft 12, and the insertion rod 9 is located in the slot 14, and the depth of the slot 14 is greater than the sum of the length of the insertion rod 9 and the thickness of the clamping ring 10. Ear blocks 15 are fixed on opposite sides of the outer wall of the support cover 11, a through hole 16 is provided on one opposite side of the inside of the clamping ring 10, and a groove 17 is provided on the other opposite side of the inside of the clamping ring 10, and the ear block 15 is located in the groove 17 after passing through the through hole 16.

[0042] When the crushing is completed, the sample barrel 3 can be removed from the support cover 11 by rotating it. At this time, the support cover 11 can be pushed to drive the ear block 15 to move up, and then the support rod is rotated to make the ear block 15 coincide with the position of the through hole 16, and finally the support cover 11 is moved down to remove it from the clamping ring 10. During installation, the insertion rod 9 is aligned with the slot 14, and the ear block 15 is passed through the through hole 16 and then rotated to the groove 17. At this time, the installation of the support cover 11 and the crushing knife 13 can be completed, so that its loading and unloading becomes convenient, which is beneficial to the later cleaning and avoids the contamination of the sample during the next detection, so as to ensure the accuracy of subsequent detection.

[0043] Please see attached Figure 1 , Figure 2 and Fig.11 One side of the support plate 6 is fixedly connected to the limiting plate 36, and one side of the box cover 2 is rotatably connected to the clamping rod 19, and the cross-section of the clamping rod 19 is set to be T-shaped, which is used to limit the support plate 6 after the support plate 6 is folded and stored.

[0044] By rotating the support plate 6, it can be brought into contact with the box cover 2 and fit together. At this time, the support plate 6 can be folded and stored, and then the clamping rod 19 is rotated so that one side of it contacts the outer wall of the clamping rod 19. At this time, the clamping rod 19 can be used to limit the support plate 6 to prevent it from displacement, and when the support plate 6 is opened, the support plate 6 will be in a mutually perpendicular state with the box cover 2, and at this time the limiting plate 36 will contact the box cover 2, so it can support the support plate 6 and prevent it from falling. A groove is provided on the support plate 6, which can position the test paper, so it is beneficial to place the test paper.

[0045] Please see attached Figure 1 , Figure 7 , Figure 8 , Fig. 9 and Fig.10 A motor 20 is fixed to one side of the bottom of the box body 1, a rotating plate 21 is fixed to the output end of the motor 20, a connecting rod 22 is fixed to one side of the rotating plate 21, a swing arm 23 is rotatably connected to the outer wall of the connecting rod 22, one end of the swing arm 23 is rotatably connected to a bracket 24, a support 25 is slidably connected to the outer wall of the bracket 24, a leg 26 is fixedly connected to the bottom of the support 25, the leg 26 is fixedly connected to the box body 1, and the test tube 4 is located inside the bracket 24. A pad 27 is fixedly connected to the bottom surface of the inner wall of the bracket 24, the bottom of the test tube 4 is located inside the pad 27, and a rubber ring 28 is fixedly connected to the top of the bracket 24, and the rubber ring 28 is located on the outer wall of the test tube 4.

[0046] After the extract and the sample are titrated into the test tube 4, the driving motor 20 drives the rotating plate 21 and the connecting rod 22 to rotate, and the connecting rod 22 drives the swing arm 23 to swing, so that the bracket 24 can reciprocate inside the support 25. At this time, the tissue fluid and the extraction solution can be mixed with each other, and the uniformity of the mixing of the two can be improved, which is conducive to the outflow of the liquid inside the tissue fluid, thereby facilitating the accuracy of the final measurement result. When the test tube 4 is placed, the test tube 4 will squeeze the rubber ring 28, so the reaction force of the rubber ring 28 can be used to clamp the test tube 4 to prevent the test tube 4 from falling off when the bracket 24 moves, and the pad 27 can be used to support the test tube 4, thereby improving the stability of the test tube 4.

[0047] Please see attached Figure 1 , Figure 5 and Figure 6 A baffle 29 is fixedly connected to the top of one side wall of the box body 1 , a sleeve 30 is fixedly connected to the bottom of the baffle 29 , the dropper 5 passes through the baffle 29 and is located in the sleeve 30 , and the support 25 is fixedly connected to the baffle 29 .

[0048] The dropper 5 can be placed by using the sleeve 30 to avoid damage caused by collision when the device is carried and moved, thereby protecting the dropper 5. The sleeve 30 can also prevent debris from entering the dropper 5, thereby preventing the dropper 5 from being contaminated and affecting the test results.

[0049] Please see attached Figure 1 and Figure 6 A storage box 34 is fixedly connected to the middle of the bottom surface of the box body 1, a box cover 35 is rotatably connected to the top of the storage box 34, and the top of the outer wall of the storage box 34 is fixedly connected to the baffle 29. A base 31 is fixedly connected to the other side wall inside the box body 1, and a storage groove 32 is opened on the top surface of the base 31. The clamp ring 10, the support cover 11 and the sample barrel 3 are all located in the storage groove 32, and a battery 33 is installed at the inner bottom of the base 31, and the battery 33 is connected to the motor 20 and the motor 1 8.

[0050] The storage box 34 can be used to store items required for testing, and when the box cover 2 is closed after the test, the snap ring 10, the support cover 11 and the sample barrel 3 will enter the storage groove 32. At this time, the snap ring 10, the support cover 11 and the sample barrel 3 will engage with the storage groove 32, thereby improving the stability of the three when moving the device.

[0051] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A rapid detection kit for okadaic acid, characterized in that: include: A box body (1), wherein a box cover (2) is rotatably connected to the opening of the box body (1), and the box body (1) (2) serves as a carrier, and a sample barrel (3), a test tube (4) and a dropper (5) are arranged inside the box body (1), wherein the sample barrel (3) is used for sampling and processing, the test tube (4) is used for placing processed samples, and the dropper (5) is used for quantitatively taking out samples from the test tube (4); A colorimetric card (7) and a foldable support plate (6) are installed on the side of the box cover (2) close to the box body (1); the support plate (6) is used to place test paper, and the colorimetric card (7) is used to perform color comparison.

2. A rapid detection kit for okadaic acid according to claim 1, characterized in that, A cover shell (18) and a snap ring (10) are fixed to the edge of the box cover (2) on one side close to the box body (1); a motor (8) is installed inside the cover shell (18); an insertion rod (9) is fixed to the output end of the motor (8); a support cover (11) is installed in the middle of the snap ring (10); the bottom of the support cover (11) is movably connected to the sample barrel (3); a rotating shaft (12) is rotatably arranged in the middle of the support cover (11); a crushing knife (13) is installed on one side of the rotating shaft (12), and the other side of the rotating shaft (12) is connected to the insertion rod (9), and the crushing knife (13) is located in the sample barrel (3).

3. A rapid detection kit for okadaic acid according to claim 2, characterized in that, A slot (14) is provided at one end of the rotating shaft (12), the insertion rod (9) is located in the slot (14), and the depth of the slot (14) is greater than the sum of the length of the insertion rod (9) and the thickness of the clamping ring (10), and one side of the support plate (6) is fixedly connected to a limiting plate (36).

4. A rapid detection kit for okadaic acid according to claim 3, characterized in that, Ear blocks (15) are fixed on opposite sides of the outer wall of the support cover (11), a through hole (16) is provided on one opposite side of the interior of the snap ring (10), a groove (17) is provided on the other opposite side of the interior of the snap ring (10), and the ear blocks (15) are located in the groove (17) after passing through the through hole (16).

5. A rapid detection kit for okadaic acid according to claim 1, characterized in that, One side of the box cover (2) is rotatably connected to a clamping rod (19), the cross section of the clamping rod (19) being arranged in a T-shape, and being used to limit the position of the support plate (6) after the support plate (6) is folded and stored.

6. A rapid detection kit for okadaic acid according to claim 2, characterized in that, A second motor (20) is fixed to one side of the bottom of the box body (1), a rotating plate (21) is fixed to the output end of the second motor (20), a connecting rod (22) is fixed to one side of the rotating plate (21), the outer wall of the connecting rod (22) is rotatably connected to a swing arm (23), one end of the swing arm (23) is rotatably connected to a bracket (24), the outer wall of the bracket (24) is slidably connected to a support (25), the bottom of the support (25) is fixedly connected to a support leg (26), the support leg (26) is fixedly connected to the box body (1), and the test tube (4) is located inside the bracket (24).

7. A rapid detection kit for okadaic acid according to claim 6, characterized in that, A pad (27) is fixedly connected to the bottom surface of the inner wall of the bracket (24), the bottom of the test tube (4) is located inside the pad (27), and a rubber ring (28) is fixedly connected to the top of the bracket (24), and the rubber ring (28) is located on the outer wall of the test tube (4).

8. A rapid detection kit for okadaic acid according to claim 6, characterized in that, A baffle (29) is fixedly connected to the top of one side wall of the box body (1), a sleeve (30) is fixedly connected to the bottom of the baffle (29), the dropper (5) passes through the baffle (29) and is located in the sleeve (30), and the support (25) is fixedly connected to the baffle (29).

9. A rapid detection kit for okadaic acid according to claim 1, characterized in that, A storage box (34) is fixedly connected to the middle of the bottom surface of the box body (1), a box cover (35) is rotatably connected to the top of the storage box (34), and the top of the outer wall of the storage box (34) is fixedly connected to the baffle (29).

10. A rapid detection kit for okadaic acid according to claim 6, characterized in that: The other side wall inside the box body (1) is fixedly connected to a base (31), the top surface of the base (31) is provided with a storage groove (32), the clamping ring (10), the support cover (11) and the sample barrel (3) are all located in the storage groove (32), and a battery (33) is installed at the inner bottom of the base (31), and the battery (33) is connected to the second motor (20) and the first motor (8).

Images