Composition and application thereof in improvement of chicken coloring effect

Abstract

The invention relates to the technical field of biology, and particularly discloses a composition and application thereof in improving the coloring effect of chickens. The composition disclosed by the invention is prepared from lutein and ursolic acid, the mass ratio of the xanthophyll to the ursolic acid is (20-40): (4-10). The composition provided by the invention can improve the coloring effect of chickens, is purely natural, safe and non-toxic, can reduce the use of synthetic pigments, does not cause toxic or side effects on animals, is beneficial to guarantee the safety of animal products, can be used as a safe, efficient, stable and controllable green and environment-friendly feed additive, and has a very good application prospect.

Description

Technical Field

[0001] The invention relates to the field of biotechnology, in particular to a composition and application thereof in improving the coloring effect of chickens. Background Art

[0002] Influenced by traditional consumer culture, coloration has become one of the main indicators for measuring the quality of yellow-feathered broiler products, directly affecting the market price and demand for yellow-feathered broilers. Broilers cannot synthesize lutein substances that play a major role in coloring, and must be ingested from outside the body. Therefore, in actual production, chemically synthesized or natural pigments are added to the feed to improve the color of the skin, shanks, beaks and other parts of the broiler. Studies have shown that chemically synthesized carotenoids such as apomethazine and canthaxanthin have a negative impact on the growth performance, liver and kidneys of broilers during their use. However, natural pigments have problems such as unsatisfactory coloring effects and low utilization. Therefore, it is urgent to develop green and effective feed additives that are non-toxic and have no side effects, which can promote the growth of livestock and poultry and ensure coloring effects. Summary of the invention

[0003] One of the objects of the present invention is to provide a novel composition for improving the coloring effect of chicken.

[0004] The present invention provides a composition, comprising: lutein and ursolic acid; the mass ratio of the lutein to the ursolic acid is (20-40): (4-10).

[0005] The present invention finds that the combination of lutein and ursolic acid in a specific ratio can play a synergistic role in the coloring of chickens. The coloring effect of the natural pigment lutein on chickens is greatly improved. Moreover, the composition does not affect the growth performance of chickens and can be used as an ideal feed additive.

[0006] In the composition of the present invention, the mass ratio of lutein to ursolic acid is 20:(4-10). Preferably, the mass ratio of lutein to ursolic acid is 20:4 or 20:10.

[0007] In the composition of the present invention, the mass ratio of lutein to ursolic acid is (30-40):(4-7). Preferably, the mass ratio of lutein to ursolic acid is 40:4 or 30:7.

[0008] In the composition of the present invention, the mass ratio of lutein to ursolic acid is (38-40):(4-5).

[0009] There is no particular limitation on the dosage form of the composition of the present invention. The composition can be prepared as an oral preparation, preferably, a powder, granules, or pills.

[0010] The invention also provides a feed additive, which comprises the above composition.

[0011] The present invention also provides a feed, which comprises the feed additive.

[0012] The invention also provides application of the composition or feed additive or feed in improving the coloring effect of chickens.

[0013] In the application of the present invention, the coloring effect of chickens is improved by feeding the above composition to chickens. During feeding, 1-6 kg of the above composition is added to each ton of basic daily diet.

[0014] In the application of the present invention, 2-3 kg of the above composition is added to each ton of basic diet.

[0015] In the application of the present invention, improving the coloring effect of the chicken refers to increasing the chromaticity value of the chicken's legs and beak and / or increasing the degree to which the chicken's skin appears yellow.

[0016] The beneficial effects of the present invention are at least: The composition provided by the present invention is pure natural, safe and non-toxic, can reduce the use of synthetic pigments, will not cause toxic side effects to animals, is conducive to ensuring the safety of animal products, can be used as a safe, efficient, stable and controllable green and environmentally friendly feed additive, and has a good application prospect in improving the coloring effect of chickens. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 Photos of the chicken beaks being colored for each group.

[0018] Figure 2 Photos of the chicken legs in each group being colored.

[0019] Figure 3 Photo of the skin testing process under chicken wings. DETAILED DESCRIPTION

[0020] The preferred embodiments of the present invention will be described in detail below in conjunction with examples. It should be understood that the following examples are provided only for the purpose of illustration and are not intended to limit the scope of the present invention. Those skilled in the art may make various modifications and substitutions to the present invention without departing from the purpose and spirit of the present invention.

[0021] The experimental methods used in the following examples are conventional methods unless otherwise specified. The materials, reagents, etc. used in the following examples are commercially available or prepared according to conventional methods in the art unless otherwise specified.

[0022] Example 1

[0023] This embodiment provides a composition comprising the following components in parts by weight: 20 parts of lutein and 4 parts of ursolic acid.

[0024] Example 2

[0025] This embodiment provides a composition comprising the following components in parts by weight: 20 parts of lutein and 10 parts of ursolic acid.

[0026] Example 3

[0027] This embodiment provides a composition comprising the following components in parts by weight: 40 parts of lutein and 4 parts of ursolic acid.

[0028] Example 4

[0029] This embodiment provides a composition comprising the following components in parts by weight: 30 parts of lutein and 7 parts of ursolic acid.

[0030] Comparative Example 1

[0031] This comparative example provides a composition, comprising the following components in parts by weight: 24 parts of lutein and 0 parts of ursolic acid.

[0032] Comparative Example 2

[0033] This comparative example provides a composition comprising the following components in parts by weight: 15 parts of lutein and 10 parts of ursolic acid.

[0034] Comparative Example 3

[0035] This comparative example provides a composition, comprising the following components in parts by weight: 44 parts of lutein and 0 parts of ursolic acid.

[0036] Comparative Example 4

[0037] This comparative example provides a composition comprising the following components in parts by weight: 0 parts of lutein and 30 parts of ursolic acid.

[0038] Example

[0039] The feed additive composition of each embodiment and comparative example was used for broiler breeding to detect its effect on the coloring effect, growth performance, etc. of yellow-feathered broilers. In order to facilitate comparison of the effect, ordinary broiler feed of the same brand, specification and batch on the market was used in the following experimental process.

[0040] 1. Experimental design

[0041] 1296 healthy yellow-feathered broilers of 21 days of age and similar body weight were selected and randomly divided into 9 groups (blank group and Examples 1-4, Comparative Examples 1-4), each group was treated with 6 replicates, and each replicate had 24 chickens. The experimental basal feed used Nantong Zhengda 510 / 510Y series compound feed. The blank group was fed only with basal feed. The experimental groups were fed with basal feeds with feed additive compositions of each embodiment and comparative example added to the basal feed, wherein the addition amount of the feed additive composition of each embodiment and comparative example was 2kg / t basal feed. The test period was 28d.

[0042] 2. Feeding and management

[0043] The experimental broilers were raised in three-layer cages. The chicken houses and equipment were fully rinsed and strictly disinfected before the experiment. The chicken house uses an electric intelligent heater for temperature control, continuous lighting, natural ventilation and longitudinal negative pressure ventilation. During the experimental period, the lighting, relative humidity and temperature in the house were controlled according to the requirements for the feeding and management of yellow-feathered broilers. The chicken house was cleaned regularly and immunized according to the normal immunization procedure. The chickens were free to eat and drink throughout the experimental period. The temperature and humidity in the chicken house were recorded at 08:00, 12:00 and 18:00 every day. During the experiment, the feeding, drinking and health status of the experimental chickens were observed and recorded every day, and the mortality and feed consumption of the experimental chickens were recorded in time.

[0044] 3. Measurement indicators and methods

[0045] Coloring effect: During the test period, 6 broilers were randomly selected from each repetition to compare the color of the chicken legs and beaks in each group using the Roche colorimetric fan to obtain the color value. At the same time, the Lab value of the skin under the wing area on the side of the chicken was measured using a colorimetric meter. Before all Lab values ​​were measured, the colorimetric meter was calibrated with a white board and a black board. Each sample was measured 3 times at different locations, and then the average value was taken. The letters of the colorimetric meter represent the following meanings: L is lightness, a is the difference between red and green, and b is the difference between yellow and blue.

[0046] Roche colorimetric fan test: shanks and beaks. Colorimeter test: skin under the wing area on the side of the chicken.

[0047] 4. Data Statistical Analysis

[0048] SPSS 20 software was used to perform one-way analysis of variance on all experimental data.

[0049] 5. Test results

[0050] 5.1 Growth performance of yellow-feathered broiler chickens: The statistical results of the growth performance data of all experimental animals are shown in Table 1.

[0051] It can be seen from Table 1 that, compared with the blank group, adding the composition of each embodiment to the diet can improve the growth performance of yellow-feathered broilers to a certain extent.

[0052] Table 1

[0053] 5.2 Coloration effect of yellow-feathered broiler beaks

[0054] Representative photos of the beak coloring effect of each group on day 28 (one chicken was randomly selected for each replicate) are shown in Figure 1 The statistical results are shown in Table 2. It can be seen from Table 2 that adding the composition of each embodiment to the diet can improve the color value of the beak of yellow-feathered broiler chickens. Compared with the control group (blank group), the composition of each embodiment and the comparative example group significantly improved the Roche color value of the beak of broiler chickens, and the effect of each embodiment group was better than that of each comparative example group.

[0055] Table 2

[0056] 5.3 Coloring effect of yellow-feathered broiler chicken shanks

[0057] Representative photos of the chicken leg coloring effect of each group on the 28th day (one chicken leg was randomly selected for each replicate) are shown in Figure 2 The statistical results are shown in Table 3. It can be seen from Table 3 that adding the composition of each embodiment to the diet can improve the chromaticity value of the chicken shank of yellow-feathered broiler chickens. Compared with the control group (blank group), the composition of each embodiment and the comparative example group significantly improved the Roche chromaticity of the chicken shank of the broiler chickens, and the effect of each embodiment group was better than that of each comparative example group.

[0058] Table 3

[0059] 5.4 Coloring effect of the skin under the wings of yellow-feathered broiler chickens

[0060] See the photos of the skin test process under the chicken wings Figure 3 The statistical results are shown in Table 4. It can be seen from Table 4 that adding the composition of each embodiment to the diet can improve the Lab value of yellow-feathered broiler chickens. Compared with the control group (blank group), the composition of each embodiment and comparative example group significantly improved the b value of the wing skin of broiler chickens (the larger the b value, the more yellowish the color), and the effect of each embodiment group was better than that of each comparative example group.

[0061] Table 4

[0062] Although the present invention has been described in detail above with general descriptions and specific embodiments, it is obvious to those skilled in the art that some modifications or improvements may be made thereto based on the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of protection claimed by the present invention.

Claims

1. A composition, characterized in that include: Lutein and ursolic acid; the mass ratio of lutein to ursolic acid is (20-40): (4-10).

2. The composition according to claim 1, characterized in that The mass ratio of lutein to ursolic acid is 20:(4-10).

3. The composition according to claim 1, characterized in that The mass ratio of lutein to ursolic acid is (30-40):(4-7).

4. The composition according to claim 1, characterized in that The mass ratio of lutein to ursolic acid is (38-40): (4-5).

5. A feed additive, characterized in that: The invention comprises the composition according to any one of claims 1 to 4.

6. A feed, characterized in that: Including the feed additive according to claim 5.

7. Use of the composition according to any one of claims 1 to 4, the feed additive according to claim 5, or the feed according to claim 6 in improving the coloring effect of chickens.

8. The use according to claim 7, characterized in that: The coloring effect of chickens is improved by feeding the composition described in any one of claims 1 to 4 to chickens. During feeding, 1 to 6 kg of the composition described in any one of claims 1 to 4 is added to each ton of basic diet.

9. The use according to claim 8, characterized in that: Add 2-3 kg of the composition according to any one of claims 1-4 to each ton of basic diet.

10. The use according to any one of claims 7 to 9, characterized in that: Improving the coloring effect of the chicken means increasing the chromaticity value of the chicken's legs and beaks and / or increasing the degree of yellowness of the chicken's skin.

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