Composition with anti-allergy, repairing and moisturizing effects and application thereof

Abstract

The invention relates to a composition with anti-allergy, repairing and moisturizing effects. The composition is prepared from fibronectin, kava pepper leaf / root / stem extract, ectoine, hydroxyphenyl propionamide benzoic acid, wheat fine powder lipid and bio-saccharide gum-1. The specific components are matched with one another, inflammatory response can be inhibited from multiple aspects, the skin can be sufficiently relieved, the redness phenomenon is reduced, meanwhile, the skin barrier is enhanced and repaired, long-term moisturizing is achieved, and the excellent anti-allergy, repairing and moisturizing effects are achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a composition having allergy-relieving, repairing and moisturizing effects and application thereof. Background Art

[0002] As the largest organ in the body, the skin is constantly interacting with the external environment. However, external factors such as environmental pollution, ultraviolet radiation, cosmetic residues, and internal factors such as lifestyle habits and endocrine disorders can damage the skin and cause various skin problems, such as sensitivity, dryness, redness, acne, etc. With the development of cosmetic science research and the growing demand of consumers for cosmetic efficacy, soothing skin sensitivity and discomfort, repairing damaged skin barriers, and enhancing skin resistance have become one of the main demands of consumers.

[0003] There are quite a few cosmetic products with repairing and soothing effects on the market, but the actual effects vary, and there is a lack of products with good repairing and soothing effects. Generally, soothing and repairing products mostly achieve soothing effects such as immediate redness fading through a few pathways (such as antihistamines), but this can only effectively inhibit the skin erythema, edema, and itching caused by the inflammatory response affected by the corresponding pathways. The skin cannot be truly soothed in the face of sensitive stimuli under complex circumstances, and it cannot subsequently repair the barrier from the inside out and strengthen the skin. The real repairing and soothing effects often need to be achieved through medical beauty methods, such as photorejuvenation and microneedle treatment, but this method will bring consumers a certain degree of pain and discomfort, and sometimes medical beauty accidents will occur.

[0004] Therefore, providing a composition that can achieve true soothing and repairing effects and can be used safely alone is a technical problem that needs to be solved urgently in the art. Summary of the invention

[0005] In order to solve the above technical problems, the present invention provides a composition having soothing, repairing and moisturizing effects and its application. The specific components in the present invention cooperate with each other to inhibit inflammatory response from multiple aspects, fully soothe the skin and reduce redness, while strengthening and repairing the skin barrier, and long-term moisturizing, with excellent soothing, repairing and moisturizing effects.

[0006] To achieve this object, the present invention adopts the following technical solutions:

[0007] In a first aspect, the present invention provides a composition having soothing, repairing and moisturizing effects, the composition comprising fibronectin, Kava Piper melongena leaf / root / stem extract, ectoine, hydroxyphenylpropionamide benzoic acid, wheat powder lipid and biosaccharide gum-1.

[0008] In the present invention, fibronectin is a high molecular weight glycoprotein in the extracellular matrix, a protein active substance in the extracellular matrix widely present in the human body, and the main non-collagenous glycoprotein in the intercellular matrix and basement membrane of the skin; it plays a central role in cell adhesion, can regulate cell polarity, differentiation and growth, can stimulate fibroblasts to synthesize collagen, and can quickly aggregate coagulation factors and various growth factors at micro-wounds in the skin, participate in hemostasis and accelerate wound healing; through the adhesion effect on cells, it can create a growth microenvironment for cells in a short period of time, assist cells and matrix to combine with each other, and accelerate the repair of the stratum corneum barrier and the recovery of the skin's internal environment.

[0009] In the present invention, the extract of Piper melongena leaf / root / stem mainly contains kavalactones, kavalpyrones, etc., and the kavalactones include capsaicin, dihydrocapsaicin, capsaicin bitter, dihydrocapsaicin bitter, methoxycapsaicin and demethoxycapsaicin; it has a significant anti-inflammatory effect, can effectively control tumor necrosis factor, thereby reducing the inflammatory response caused by contact with a large amount of compounds and ultraviolet rays; in animal experiments, it can even make mice immune to inflammatory responses that are usually fatal, has excellent ability to calm the skin and relieve discomfort, can inhibit the excessive response of sensory nerves, thereby significantly improving the skin's tolerance to external stimuli, and can also block the transmission of information by neurons through capsaicin occupying skin receptors and inhibiting the release of inflammatory mediators, thereby inhibiting the transmission of sensitivity.

[0010] In the present invention, Ecdoin is a very hydrophilic substance that can form a stable protective film around skin cells and proteins, effectively lock in moisture, reduce transepidermal water loss, and improve the hydration level of the skin; it can reduce ultraviolet damage by regulating the number of epidermal melanocytes, thereby preventing photoaging; it can enhance fibroblast activity, promote collagen synthesis, repair damaged skin cells, and promote the skin's regeneration process. This repair effect is not limited to the epidermis, but can also penetrate into the dermis, providing comprehensive repair and care for the skin; it has certain anti-inflammatory properties, can inhibit the expression of pro-inflammatory genes (such as ICAM-1), reduce the expression of inflammation-related proteins (such as Hsp70s), and the production and release of multiple inflammatory factors (such as NO), reduce the inflammatory response of the skin, and soothe irritated and damaged skin.

[0011] In the present invention, hydroxyphenylpropionamide benzoic acid can inhibit the release of histamine from mast cells, and effectively inhibit skin erythema, edema and itching caused by histamine; it has multifunctional anti-inflammatory activity, including inhibiting the degradation of keratinocyte nuclear factor NF-κB-α which is directly related to the occurrence of inflammation, preventing the phosphorylation of p65 protein subunit on the nuclear factor NF-κB, thereby blocking the occurrence process of cell inflammation, reducing the occurrence of inflammatory immune response and the reaction of skin neurodermatitis; it can inhibit the activity of NF-κB degradation enzyme induced by tumor necrosis factor TNF-α, and reduce the release of inflammatory factors and IL-8 that causes atopic dermatitis; it has strong anti-inflammatory and antipruritic properties, and is a strong non-steroidal anti-inflammatory and antipruritic natural ingredient, with an efficacy equivalent to that of hydrocortisone, but without any side effects of steroid hormones.

[0012] In the present invention, the wheat fine powder lipid can simulate the lipid structure of the skin itself, especially the composition and function of intercellular lipids (mainly composed of ceramide, free fatty acids and cholesterol), which helps to supplement the missing lipid components in the skin, thereby strengthening the integrity of the skin barrier; it has a good effect on the proliferation of fibroblasts and can promote the metabolism and renewal of skin cells; it can also regulate the microecological environment of the skin and inhibit the growth of harmful bacteria, thereby maintaining the health of the skin; in addition, it has good moisturizing and nourishing properties, can lock the moisture in the skin, prevent moisture loss, and keep the skin hydrated.

[0013] In the present invention, biosaccharide gum-1 contains high molecular weight polysaccharides (1Mda), which can absorb water even in a low humidity environment and form a breathable film on the skin surface; it is easily degraded by skin enzymes into smaller molecular sugars to naturally capture water molecules and has a long-lasting moisturizing effect; it competitively inhibits MIF (macrophage migration inhibitory factor) to promote the removal of inactive macrophages from the infected site. MIF can fix itself on the receptors of macrophages or on the fucose contained in biosaccharide gum-1. When it is fixed on fucose, the macrophages will not be activated by MIF and remain in an inert state, so that they can be removed from the infected site.

[0014] In the present invention, kava pepper leaf / root / stem extract, ectoine, hydroxyphenylpropionamide benzoic acid and biosaccharide gum-1 are used in combination, which has a synergistic technical effect in soothing; fibronectin, ectoine and wheat fine powder lipid are used in combination, which has a synergistic technical effect in repairing the skin barrier; ectoine and biosaccharide gum-1 are used in combination, which has a synergistic technical effect in moisturizing. The above-mentioned specific ingredients cooperate with each other to inhibit inflammatory reactions from many aspects, fully soothe the skin and reduce redness, while strengthening and repairing the skin barrier, moisturizing for a long time, and having excellent soothing, repairing and moisturizing effects.

[0015] Preferably, the composition comprises, by mass, 0.00005-0.0025 parts of fibronectin (for example, 0.0001 parts, 0.0005 parts, 0.001 parts, 0.0015 parts, 0.002 parts, etc.), 0.003-0.09 parts of Kava pepper leaf / root / stem extract (for example, 0.01 parts, 0.02 parts, 0.04 parts, 0.06 parts, 0.08 parts, etc.), 0.1-3 parts of ectoine (for example, It can be 0.5 parts, 1 parts, 1.5 parts, 2 parts, 2.5 parts, etc.), hydroxyphenylpropionamide benzoic acid 0.001-0.1 parts (for example, it can be 0.005 parts, 0.01 parts, 0.02 parts, 0.04 parts, 0.06 parts, 0.08 parts, etc.), wheat flour lipid 0.01-0.2 parts (for example, it can be 0.05 parts, 0.075 parts, 0.1 parts, 0.125 parts, 0.15 parts, 0.175 parts, etc.) and biosaccharide gum-1 0.001-0.05 parts (for example, it can be 0.005 parts, 0.01 parts, 0.02 parts, 0.03 parts, 0.04 parts, etc.).

[0016] Preferably, the composition further comprises any one of Astragalus membranaceus root extract, Saposhnikovia root extract, Calendula officinalis flower extract, Albizzia julibrissin flower extract or Gastrodia elata root extract, or a combination of at least two thereof.

[0017] Preferably, the composition further comprises a combination of Astragalus membranaceus root extract, Saposhnikovia root extract, Calendula officinalis flower extract, Albizzia julibrissin flower extract and Gastrodia elata root extract.

[0018] In the present invention, the above-mentioned plant extracts are rich in compounds such as gastrodin, flavonoids, coumarins, chromones, etc., which can inhibit the secretion of pro-inflammatory mediators and the exudation of inflammatory substances, thereby reducing the inflammatory response; by affecting the metabolism and function of vascular endothelial cells, the stability of the blood vessel wall is enhanced, thereby reducing the permeability of capillaries; the polysaccharides and carotenoid compounds contained therein can help repair damaged epidermal cells, promote the skin's self-repair ability, and strengthen the skin's barrier function, thereby reducing the loss of water from the skin after allergic reactions.

[0019] Preferably, the composition further comprises, by mass, 0.01-0.25 parts of Astragalus membranaceus root extract (for example, 0.05 parts, 0.1 parts, 0.15 parts, 0.2 parts, etc.), 0.01-0.1 parts of Saposhnikovia root extract (for example, 0.02 parts, 0.05 parts, 0.06 parts, 0.08 parts, etc.), 0.005-0.1 parts of Calendula officinalis flower extract (for example, 0.02 parts, 0.04 parts, 0.06 parts, 0.08 parts, etc.), 0.0005-0.04 parts of Albizzia julibrissin flower extract (for example, 0.001 parts, 0.005 parts, 0.01 parts, 0.02 parts, 0.03 parts, etc.), and 0.0005-0.04 parts of Gastrodia elata root extract (for example, 0.001 parts, 0.005 parts, 0.01 parts, 0.02 parts, 0.03 parts, etc.).

[0020] Preferably, the composition further comprises any one of Centella asiatica extract, Polygonum cuspidatum root extract, Scutellaria baicalensis root extract, Camellia oleifera extract, Glycyrrhiza glabra root extract, Chamomile flower extract or Rosemary leaf extract, or a combination of at least two thereof.

[0021] Preferably, the composition further comprises a combination of Centella asiatica extract, Polygonum cuspidatum root extract, Scutellaria baicalensis root extract, Camellia sinensis leaf extract, Glycyrrhiza glabra root extract, Chamomile flower extract and Rosemary leaf extract.

[0022] In the present invention, the above-mentioned plant extracts can inhibit the production of inflammatory mediators, such as prostaglandins, leukotrienes, etc., and can effectively reduce inflammatory reactions; they can inhibit the activity of enzymes related to inflammatory reactions, such as cyclooxygenase, lipoxygenase, etc., and can reduce the synthesis and release of inflammatory mediators; they can promote collagen synthesis and stimulate cell proliferation, accelerate the healing process of damaged skin tissue, and repair the skin barrier; the natural moisturizing ingredients therein, such as amino acids, sugars, alcohols, etc., can form a moisturizing barrier by adsorbing, binding or locking water on the skin surface to prevent rapid evaporation of water.

[0023] Preferably, the composition further comprises, by mass, 0.0005-0.1 parts of Centella asiatica extract (for example, 0.001 parts, 0.005 parts, 0.01 parts, 0.05 parts, 0.075 parts, etc.), 0.0005-0.1 parts of Polygonum cuspidatum root extract (for example, 0.001 parts, 0.005 parts, 0.01 parts, 0.05 parts, 0.075 parts, etc.), 0.0005-0.1 parts of Scutellaria baicalensis root extract (for example, 0.001 parts, 0.005 parts, 0.01 parts, 0.05 parts, 0.075 parts, etc.), and 0.0005-0.1 parts of Tea extract (for example, 0.001, 0.005, 0.01, 0.05, 0.075, etc.), 0.0005-0.1 parts of Glycyrrhiza glabra root extract (for example, 0.001, 0.005, 0.01, 0.05, 0.075, etc.), 0.0005-0.1 parts of Matricaria flower extract (for example, 0.001, 0.005, 0.01, 0.05, 0.075, etc.), and 0.0005-0.1 parts of Rosemary leaf extract (for example, 0.001, 0.005, 0.01, 0.05, 0.075, etc.).

[0024] In a second aspect, the present invention provides a use of the composition having soothing, repairing and moisturizing effects according to the first aspect in the preparation of cosmetics.

[0025] Preferably, the mass fraction of the composition in the cosmetic is 0.01-5% (for example, it can be 0.05%, 0.1%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, etc.).

[0026] Preferably, the cosmetics include facial masks, lotions, toners, creams, essences, make-up removers or cleansing products.

[0027] In a third aspect, the present invention provides an essence having soothing, repairing and moisturizing effects, wherein the essence comprises a skin conditioner, a chelating agent, a moisturizer, a thickener, a pH neutralizer and water; and the skin conditioner comprises the composition having soothing, repairing and moisturizing effects as described in the first aspect.

[0028] Preferably, the essence comprises, by mass percentage, 0.01-3% of a skin conditioning agent (for example, 0.05%, 0.1%, 1%, 1.5%, 2%, 2.5%, etc.), 0.01-0.2% of a chelating agent (for example, 0.02%, 0.05%, 0.08%, 0.1%, 0.12%, 0.15%, 0.18%, etc.), 1-20% of a moisturizing agent (for example, 2%, 5%, 8%, 10%, 12%, 15%, 18%, etc.), thickener 0.1-2% (for example, 0.2%, 0.5%, 1%, 1.2%, 1.5%, 1.8%, etc.), pH neutralizer 0.01-1% (for example, 0.05%, 0.1%, 0.2%, 0.5%, 0.8%, etc.) and water 75-95% (for example, 76%, 80%, 84%, 88%, 92%, etc.).

[0029] Preferably, the chelating agent comprises disodium EDTA.

[0030] Preferably, the moisturizing agent includes any one of trehalose, betaine, sodium hyaluronate, panthenol, goat milk extract, glycosphingolipids, euglena polysaccharide, butylene glycol, glycerol, 1,2-pentanediol, 1,2-hexanediol, propylene glycol or methylpropanediol, or a combination of at least two thereof.

[0031] Preferably, the thickener includes any one or a combination of at least two of carbomer, xanthan gum, cellulose, ammonium acrylamide dimethyl taurate / VP copolymer, hydroxyethyl acrylate / sodium acryloyl dimethyl taurate copolymer-squalane-polysorbate-60, and acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer.

[0032] Preferably, the pH neutralizer comprises any one of aminopropanol or triethanolamine or a combination of both.

[0033] Preferably, the essence further comprises a preservative.

[0034] Preferably, the essence further comprises 0.05-1.5% (eg 0.1%, 0.5%, 0.8%, 1%, 1.2%, etc.) of preservatives in terms of mass percentage.

[0035] Preferably, the preservative comprises any one of p-hydroxyacetophenone, phenoxyethanol, caprylhydroxamic acid or o-cymene-5-ol, or a combination of at least two thereof.

[0036] In a fourth aspect, the present invention provides a method for preparing the essence having soothing, repairing and moisturizing effects according to the third aspect, the preparation method comprising the following steps:

[0037] The substances that need to be dissolved at high temperature among the chelating agent, thickener and moisturizing agent are mixed with water, heated and stirred until completely dissolved, and then the temperature is lowered for the first time, a pH neutralizer and an optional preservative are added, followed by a second cooling, and the remaining components of the skin conditioner and moisturizing agent are added to obtain the essence.

[0038] The substances that need to be dissolved at high temperature in the moisturizing agent of the present invention include, for example, trehalose, betaine, sodium hyaluronate, butylene glycol, glycerin, methylpropanediol, and propylene glycol.

[0039] Preferably, the temperature is raised to 80-85°C (eg, 81°C, 82°C, 83°C, 84°C, etc.).

[0040] Preferably, the first cooling temperature is 50-55°C (eg, 51°C, 52°C, 53°C, 54°C, etc.).

[0041] Preferably, the second cooling temperature is 25-35°C (for example, 26°C, 28°C, 30°C, 32°C, 34°C, etc.).

[0042] Compared with the prior art, the present invention has at least the following beneficial effects:

[0043] In the present invention, the compound use of Kava pepper leaf / root / stem extract, ectoine, hydroxyphenylpropionamide benzoic acid and biosaccharide gum-1 has a synergistic technical effect in soothing; the compound use of fibronectin, ectoine and wheat fine powder lipid has a synergistic technical effect in repairing the skin barrier; the compound use of ectoine and biosaccharide gum-1 has a synergistic technical effect in moisturizing. The above-mentioned specific ingredients cooperate with each other to inhibit inflammatory reactions from many aspects, fully soothe the skin and reduce redness, while strengthening and repairing the skin barrier, moisturizing for a long time, and having excellent soothing, repairing and moisturizing effects. DETAILED DESCRIPTION

[0044] For the convenience of understanding the present invention, the present invention lists the following embodiments. It should be understood by those skilled in the art that the embodiments are only to help understand the present invention and should not be regarded as specific limitations of the present invention.

[0045] The sources of raw materials used in the following examples and comparative examples are as follows:

[0046] Fibronectin was purchased from Shanghai Haotai Biotechnology Co., Ltd.;

[0047] Wheat powder lipids were purchased from Guangzhou Bochi Biotechnology Co., Ltd.;

[0048] Biosaccharide gum-1 was purchased from France's Cintin Company;

[0049] Astragalus membranaceus root extract, siler root extract, calendula officinalis flower extract, julibrissin flower extract, and gastrodia elata root extract were purchased from Miaosen Co., Ltd.;

[0050] Centella asiatica extract, Polygonum cuspidatum root extract, Scutellaria baicalensis root extract, tea extract, Glycyrrhiza glabra root extract, Chamomile flower extract, and Rosemary leaf extract were purchased from Guangzhou Peitao Company;

[0051] The weight percentages of the raw materials mentioned above are all calculated based on the actual content of the effective ingredients contained in the commercially available raw materials. The commercially available raw materials may also selectively contain any one or a combination of at least two of solvents, fillers, diluents, stabilizers, pH adjusters, antibacterial agents, antioxidants or impurities within the permitted range.

[0052] Other raw materials are common commercially available products and will not be described in detail here.

[0053] Example 1

[0054] The present embodiment provides a composition with soothing, repairing and moisturizing effects, which comprises, by mass, 0.001 parts of fibronectin, 0.05 parts of kava pepper leaf / root / stem extract, 1.5 parts of ectoine, 0.05 parts of hydroxyphenylpropionamide benzoic acid, 0.1 parts of wheat fine powder lipid, 0.02 parts of biosaccharide gum-1, 0.15 parts of Astragalus membranaceus root extract, 0.08 parts of Saposhnikovia root extract, 0.05 parts of Calendula officinalis flower extract, 0.025 parts of Albizzia julibrissin flower extract, 0.025 parts of Gastrodia elata root extract, 0.05 parts of Centella asiatica extract, 0.05 parts of Polygonum cuspidatum root extract, 0.05 parts of Scutellaria baicalensis root extract, 0.05 parts of tea extract, 0.05 parts of Glycyrrhiza glabra root extract, 0.05 parts of Matricaria flower extract and 0.05 parts of Rosemary leaf extract.

[0055] Example 2

[0056] The present embodiment provides a composition with soothing, repairing and moisturizing effects, which comprises, by mass, 0.00005 parts of fibronectin, 0.003 parts of kava pepper leaf / root / stem extract, 0.1 parts of ectoine, 0.001 parts of hydroxyphenylpropionamide benzoic acid, 0.01 parts of wheat fine powder lipid, 0.001 parts of biosaccharide gum-1, 0.03 parts of Astragalus membranaceus root extract, 0.016 parts of Saposhnikovia root extract, 0.01 parts of Calendula officinalis flower extract, 0.005 parts of Albizzia julibrissin flower extract, 0.005 parts of Gastrodia elata root extract, 0.0005 parts of Centella asiatica extract, 0.0005 parts of Polygonum cuspidatum root extract, 0.0005 parts of Scutellaria baicalensis root extract, 0.0005 parts of tea extract, 0.0005 parts of Glycyrrhiza glabra root extract, 0.0005 parts of Matricaria flower extract and 0.0005 parts of Rosemary leaf extract.

[0057] Example 3

[0058] The present embodiment provides a composition with soothing, repairing and moisturizing effects, which comprises, by mass, 0.0025 parts of fibronectin, 0.09 parts of kava pepper leaf / root / stem extract, 3 parts of ectoine, 0.1 parts of hydroxyphenylpropionamide benzoic acid, 0.2 parts of wheat fine powder lipid, 0.05 parts of biosaccharide gum-1, 0.19 parts of Astragalus membranaceus root extract, 0.1 parts of Saposhnikovia root extract, 0.065 parts of Calendula officinalis flower extract, 0.0325 parts of Albizzia julibrissin flower extract, 0.0325 parts of Gastrodia elata root extract, 0.1 parts of Centella asiatica extract, 0.1 parts of Polygonum cuspidatum root extract, 0.1 parts of Scutellaria baicalensis root extract, 0.1 parts of tea extract, 0.1 parts of Glycyrrhiza glabra root extract, 0.1 parts of Chamomile flower extract and 0.1 parts of Rosemary leaf extract.

[0059] Example 4

[0060] This embodiment provides a composition with soothing, repairing and moisturizing effects, which is different from Example 1 only in that it does not contain Astragalus membranaceus root extract, Saposhnikovia root extract, Calendula officinalis flower extract, Albizzia julibrissin flower extract, Gastrodia elata root extract, Centella asiatica extract, Polygonum cuspidatum root extract, Scutellaria baicalensis root extract, Camellia oleifera root extract, Glycyrrhiza glabra root extract, Chamomile flower extract and Rosemary leaf extract, and the mass fractions of the above substances are distributed according to the proportions of fibronectin, Piper membranaceus leaf / root / stem extract, Ectoin, hydroxyphenylpropionamide benzoic acid, wheat powder lipid and biosaccharide gum-1.

[0061] Comparative Example 1

[0062] This comparative example provides a composition with soothing, repairing and moisturizing effects, which is different from Example 4 only in that it does not contain Kava Piper methysticum leaf / root / stem extract, and its mass fraction is distributed according to the ratio of ectoine, hydroxyphenylpropionamide benzoic acid and biosaccharide gum-1.

[0063] Comparative Example 2

[0064] This comparative example provides a composition with soothing, repairing and moisturizing effects, which is different from Example 4 only in that it does not contain ectoine, and its mass fraction is distributed according to the ratio of Kava Piper methysticum leaf / root / stem extract, hydroxyphenylpropionamide benzoic acid and biosaccharide gum-1.

[0065] Comparative Example 3

[0066] This comparative example provides a composition with soothing, repairing and moisturizing effects, which is different from Example 4 only in that it does not contain hydroxyphenylpropionamide benzoic acid, and its mass fraction is distributed according to the ratio of ectoine, kava pepper leaf / root / stem extract and biosaccharide gum-1.

[0067] Comparative Example 4

[0068] This comparative example provides a composition with soothing, repairing and moisturizing effects, which is different from Example 4 only in that it does not contain biosaccharide gum-1, and its mass fraction is distributed according to the ratio of ectoine, hydroxyphenylpropionamide benzoic acid and kava pepper leaf / root / stem extract.

[0069] Comparative Example 5

[0070] This comparative example provides a composition having soothing, repairing and moisturizing effects, which is different from Example 4 only in that it does not contain fibronectin, and its mass fraction is distributed according to the ratio of ectoine and wheat powder lipid.

[0071] Comparative Example 6

[0072] This comparative example provides a composition having soothing, repairing and moisturizing effects, and the only difference between it and Example 4 is that it does not contain ectoine, and its mass fraction is distributed according to the ratio of fibronectin and wheat powder lipid.

[0073] Comparative Example 7

[0074] This comparative example provides a composition having soothing, repairing and moisturizing effects, which is different from Example 4 only in that it does not contain wheat powder lipid, and its mass fraction is distributed according to the ratio of ectoine and fibronectin.

[0075] Comparative Example 8

[0076] This comparative example provides a composition having soothing, repairing and moisturizing effects, which is different from Example 4 only in that it does not contain ectoine, and its mass fraction is allocated to biosaccharide gum-1.

[0077] Comparative Example 9

[0078] This comparative example provides a composition having soothing, repairing and moisturizing effects, which is different from Example 4 only in that it does not contain biosaccharide gum-1, and its mass fraction is allocated to ectoine.

[0079] Application Example 1

[0080] This application example provides an essence with soothing, repairing and moisturizing effects. The formula of the essence is as follows:

[0081]

[0082] The preparation method of the essence comprises the following steps:

[0083] The chelating agent, thickener, butylene glycol in the moisturizing agent, sodium hyaluronate and water are mixed, heated to 82° C., stirred until completely dissolved, then cooled to 52° C., a pH neutralizer and a preservative are added, then cooled to 30° C., and the remaining components in the skin conditioner and moisturizing agent are added to obtain the essence.

[0084] Application Example 2

[0085] This application example provides an essence with soothing, repairing and moisturizing effects. The formula of the essence is as follows:

[0086]

[0087] The preparation method of the essence comprises the following steps:

[0088] The chelating agent, thickener, butylene glycol in the moisturizing agent, sodium hyaluronate and water are mixed, heated to 80°C, stirred until completely dissolved, then cooled to 50°C, a pH neutralizer and a preservative are added, then cooled to 25°C, and the remaining components in the skin conditioner and moisturizing agent are added to obtain the essence.

[0089] Application Example 3

[0090] This application example provides an essence with soothing, repairing and moisturizing effects. The formula of the essence is as follows:

[0091]

[0092] The preparation method of the essence comprises the following steps:

[0093] The chelating agent, thickener, butylene glycol in the moisturizing agent, sodium hyaluronate and water are mixed, heated to 85°C, stirred until completely dissolved, then cooled to 55°C, a pH neutralizer and a preservative are added, then cooled to 35°C, and the remaining components in the skin conditioner and moisturizing agent are added to obtain the essence.

[0094] Application Example 4, Comparative Application Examples 1-9

[0095] The above application examples and comparative application examples respectively provide an essence with soothing, repairing and moisturizing effects, which differs from application example 1 only in that the skin conditioner is replaced by the composition provided in Example 4 and comparative examples 1-9 in equal amounts; the preparation method refers to application example 1.

[0096] Test Example 1

[0097] Allergy relief test

[0098] Test Method

[0099] TNF-α inhibition rate test: Take the same amount of the essence prepared in Examples 1-4 and Comparative Examples 1-4, dissolve them in dimethyl sulfoxide, shake and mix thoroughly, and prepare the example experimental product with a dose of 2000μg / mL, and set up a negative control group (equal amount of cell culture medium containing LPS) and a positive control group (adding 100μg / mL dexamethasone to the cell culture medium with equal amount of LPS). Place normal human dermal cells in the above 8 groups of culture fluids, and use high-glucose culture medium to culture for 24 hours under saturated humidity, constant temperature of 37°C, and 5v% carbon dioxide. After the culture is completed, the TNF-α content is determined and the TNF-α inhibition rate compared with the negative control group is calculated. The TNF-α inhibition rate test results are shown in Table 1.

[0100] Table 1

[0101]

[0102]

[0103] The test results show that:

[0104] It can be seen from the comparison between Application Example 4 and Comparative Application Examples 1-4 that the present invention has an excellent allergy-relieving effect by rationally compounding Kava Piper methysticum leaf / root / stem extract, Ectoin, hydroxyphenylpropionamide benzoic acid and biosaccharide gum-1;

[0105] By comparing Application Example 1 and Application Example 4, it can be seen that when specific plant extracts are added to the ingredients, the soothing effect is further improved;

[0106] Comprehensive test results show that Kava leaf / root / stem extract effectively controls tumor necrosis factor, occupies skin receptors and inhibits the release of inflammatory mediators to block neuronal information transmission and calm the skin; Ecdoin can inhibit the expression of pro-inflammatory genes, reduce the expression of inflammation-related proteins, inhibit the production and release of multiple inflammatory factors, reduce the inflammatory response of the skin, and soothe irritated skin; Hydroxyphenylpropionamide benzoic acid inhibits the release of histamine from mast cells and the degradation of keratinocyte nuclear factor NF-κB-α, blocking the occurrence of cellular inflammation; Biosaccharide Gum-1 competitively inhibits MIF to promote the removal of blunt macrophages from the infected site , reducing inflammatory response; plant extract combination 1 (Astragalus membranaceus root extract, Saposhnikovia root extract, Calendula officinalis flower extract, Albizzia julibrissin flower extract and Gastrodia elata root extract) inhibits the secretion of pro-inflammatory mediators and the exudation of inflammatory substances, and enhances the stability of blood vessel walls; plant extract combination 2 (Centella asiatica extract, Polygonum cuspidatum root extract, Scutellaria baicalensis root extract, Camellia sinensis leaf extract, Glycyrrhiza glabra root extract, Chamomile flower extract and Rosmarinus officinalis leaf extract) can inhibit the production of inflammatory mediators and the activity of enzymes related to inflammatory response; the above six are combined synergistically to effectively inhibit inflammatory response from multiple aspects, fully soothe the skin and reduce redness.

[0107] Test Example 2

[0108] Repair effect test

[0109] Test method: Transepidermal water loss (TEWL) test

[0110] (1) 70 volunteers with sensitive skin were selected, with an average age of 29.5 years, meeting the inclusion and exclusion criteria of the "Technical Specifications for Safety of Cosmetics". The volunteers were randomly divided into 7 groups, and the essences prepared in Application Examples 1-4 and Comparative Application Examples 5-7 were used respectively.

[0111] (2) Skin elasticity is measured using the German Tewameter (open measuring instrument). The basic principle of its measurement technology is based on the water vapor pressure gradient near the epidermis (within about 1 cm). A capacitive sensor is usually used to measure the water vapor pressure within this range, and then the amount of transdermal water evaporated is calculated.

[0112] (3) Test process: After cleansing, apply the corresponding essence of the experimental group evenly on the face twice a day for 28 consecutive days. The transepidermal water loss (TEWL) value was tested before use of the product, on the 14th day of use, and on the 28th day of use. The test results are shown in Table 2.

[0113] Table 2

[0114]

[0115]

[0116] The test results show that:

[0117] It can be seen from the comparison between Application Example 4 and Comparative Application Examples 5-7 that the present invention has an excellent repair effect by rationally compounding fibronectin, ectoine and wheat powder lipid;

[0118] By comparing Application Example 1 and Application Example 4, it can be seen that when specific plant extracts are added to the ingredients, the repair effect is further improved;

[0119] Comprehensive test results show that fibronectin regulates cell polarity, differentiation and growth, stimulates fibroblasts to synthesize collagen, accelerates wound healing, promotes stratum corneum barrier repair and recovery of the skin's internal environment; Ecdoin enhances fibroblast activity, promotes collagen synthesis, repairs damaged skin cells, and the repair effect penetrates into the dermis to provide comprehensive repair and care for the skin; wheat powder lipids can simulate the lipid structure of the skin itself, supplement the missing lipid components in the skin, and regulate the skin's microecological environment and inhibit the growth of harmful bacteria; plant extract combination 1 (Astragalus membranaceus root extract, Saposhnikovia root extract, Calendula officinalis flower extract, Albizzia julibrissin flower extract and Gastrodia elata root extract) can help repair damaged epidermal cells, promote the skin's self-repair ability, and strengthen the skin's barrier function; the above four are combined synergistically, and have a technical effect of significantly enhancing the integrity of the skin barrier.

[0120] Test Example 3

[0121] Moisturizing effect test

[0122] Test method: Stratum corneum water content (MMV) test

[0123] 30 volunteers were selected with an average age of 30.4 years old, who met the inclusion and exclusion criteria of the "Technical Specifications for Safety of Cosmetics". A 3cm×3cm test area was marked on the inside of the left and right arms of the volunteers. Multiple areas can be marked on the same arm at the same time, with an interval of 1cm between the areas. Application Examples 1-4 and Comparative Application Examples 8-9 and blank controls were randomly distributed on the left and right arms. A capacitive skin tester was used to measure the test area and the control area, and each area was measured 5 times in parallel. First, the blank value of each test area was measured, and then 2.0mg±0.1mg sample / cm 2 The test product was evenly applied to the test area using a latex fingertip. After application, the skin moisture content of the test area and the blank control area was measured at 2h, 4h and 8h respectively. The results of the stratum corneum moisture content (MMV) test are shown in Table 3.

[0124] Table 3

[0125] Grouping Before use (%) Use 2h (%) Use 4h (%) Use 8h (%) Application Example 1 27.12 65.65 63.85 60.69 Application Example 2 27.24 65.12 63.45 60.13 Application Example 3 27.03 66.07 64.42 61.24 Application Example 4 27.26 62.85 59.96 57.36 Comparative Application Example 8 27.04 59.58 56.45 53.68 Comparative Application Example 9 27.33 58.38 56.22 54.26

[0126] The test results show that:

[0127] It can be seen from the comparison between Application Example 4 and Comparative Application Examples 8-9 that the present invention has excellent moisturizing effect by rationally compounding Ecdoin and Biosaccharide Gum-1;

[0128] By comparing Application Example 1 and Application Example 4, it can be seen that when specific plant extracts are added to the ingredients, the moisturizing effect is further improved;

[0129] Comprehensive test results show that Ectoin forms a stable protective film around skin cells and proteins, effectively locking in moisture and improving the hydration level of the skin; Biosaccharide Gum-1 can form a breathable film on the skin surface, which can naturally capture water molecules after being degraded by skin enzymes into smaller molecular sugars, and has a long-lasting moisturizing effect; the natural moisturizing ingredients contained in plant extract combination 2 (Centella asiatica extract, Polygonum cuspidatum root extract, Scutellaria baicalensis root extract, Camellia oleifera root extract, Glycyrrhiza glabra root extract, Chamomile flower extract and Rosemary leaf extract) adsorb, bind or lock moisture on the skin surface to form a moisturizing barrier to prevent rapid evaporation of moisture; the above three are combined synergistically, so that the composition has excellent moisturizing effect on the basis of soothing and repairing the skin, and can continuously maintain the health of the skin after use.

[0130] Test Example 4

[0131] Safety Testing

[0132] 30 female subjects were selected as subjects. Each subject tried the essence provided in Example 1-4. A patch test tape containing 0.03 mL of the sample was applied to the normal skin of the left forearm of the subject. After removing the patch, the test was performed according to the CTFA Guidelines (1981) within half an hour. A total of 6 times were performed. On the 6th day after the patch was applied, an additional test was performed to observe the delayed reaction. The identification criteria are shown in Table 4 and the results are shown in Table 5.

[0133] Table 4

[0134] Rating scale Skin reactions 0 Negative reaction 1 Suspicious reaction, only slight erythema 2 Erythema, infiltration, edema, and papules may be present 3 Erythema, infiltration, edema, papules, prolapse; reactions may extend beyond the test area 4 Obvious erythema, severe infiltration, edema, confluent herpes; reaction beyond the test area

[0135] Table 5

[0136]

[0137]

[0138] From the above data, it can be seen that the essence of the present invention has no irritation to the skin and no positive reaction.

[0139] The applicant declares that the above is only a specific implementation mode of the present invention, but the protection scope of the present invention is not limited thereto. Those skilled in the art should understand that any changes or substitutions that can be easily thought of by those skilled in the art within the technical scope disclosed by the present invention shall fall within the protection scope and disclosure scope of the present invention.

Claims

1. A composition having soothing, repairing and moisturizing effects, characterized in that: The composition includes fibronectin, Kava leaf / root / stem extract, ectoine, hydroxyphenylpropionamide benzoic acid, wheat flour lipids and biosaccharide gum-1.

2. The composition according to claim 1, characterized in that The composition comprises, by weight, 0.00005-0.0025 parts of fibronectin, 0.003-0.09 parts of kava leaf / root / stem extract, 0.1-3 parts of ectoine, 0.001-0.1 parts of hydroxyphenylpropionamide benzoic acid, 0.01-0.2 parts of wheat powder lipid and 0.001-0.05 parts of biosaccharide gum-1.

3. The composition according to claim 1 or 2, characterized in that The composition further comprises any one or a combination of at least two of Astragalus membranaceus root extract, Parsnip root extract, Calendula officinalis flower extract, Albizzia julibrissin flower extract or Gastrodia elata root extract; Preferably, the composition further comprises a combination of Astragalus membranaceus root extract, Parsnip root extract, Calendula officinalis flower extract, Albizzia julibrissin flower extract and Gastrodia elata root extract; Preferably, the composition further comprises, by mass, 0.01-0.25 parts of Astragalus membranaceus root extract, 0.01-0.1 parts of Saposhnikovia root extract, 0.005-0.1 parts of Calendula officinalis flower extract, 0.0005-0.04 parts of Albizzia julibrissin flower extract and 0.0005-0.04 parts of Gastrodia elata root extract.

4. The composition according to any one of claims 1 to 3, characterized in that The composition further comprises any one or a combination of at least two of Centella asiatica extract, Polygonum cuspidatum root extract, Scutellaria baicalensis root extract, Camellia oleifera root extract, Glycyrrhiza glabra root extract, Chamomile flower extract or Rosemary leaf extract; Preferably, the composition further comprises a combination of Centella asiatica extract, Polygonum cuspidatum root extract, Scutellaria baicalensis root extract, Camellia sinensis leaf extract, Glycyrrhiza glabra root extract, Chamomile flower extract and Rosemary leaf extract; Preferably, the composition further comprises, by mass, 0.0005-0.1 parts of Centella asiatica extract, 0.0005-0.1 parts of Polygonum cuspidatum root extract, 0.0005-0.1 parts of Scutellaria baicalensis root extract, 0.0005-0.1 parts of tea leaf extract, 0.0005-0.1 parts of Glycyrrhiza glabra root extract, 0.0005-0.1 parts of Matricaria extract and 0.0005-0.1 parts of Rosemary leaf extract.

5. Use of the composition having soothing, repairing and moisturizing effects according to any one of claims 1 to 4 in the preparation of cosmetics; Preferably, the mass fraction of the composition in the cosmetic is 0.01-5%; Preferably, the cosmetics include facial masks, lotions, toners, creams, essences, make-up removers or cleansing products.

6. An essence with soothing, repairing and moisturizing effects, characterized in that: The essence comprises a skin conditioner, a chelating agent, a moisturizing agent, a thickener, a pH neutralizer and water; the skin conditioner comprises the composition having soothing, repairing and moisturizing effects as described in any one of claims 1 to 4.

7. The essence according to claim 6, characterized in that The essence comprises, by weight percentage, 0.01-3% of a skin conditioning agent, 0.01-0.2% of a chelating agent, 1-20% of a moisturizing agent, 0.1-2% of a thickening agent, 0.01-1% of a pH neutralizing agent and 75-95% of water.

8. The essence according to claim 6 or 7, characterized in that The chelating agent includes disodium EDTA; Preferably, the moisturizing agent comprises any one or a combination of at least two of trehalose, betaine, sodium hyaluronate, panthenol, goat milk extract, glycosphingolipids, euglena polysaccharide, butylene glycol, glycerol, 1,2-pentanediol, 1,2-hexanediol, propylene glycol or methylpropanediol; Preferably, the thickener comprises any one or a combination of at least two of carbomer, xanthan gum, cellulose, ammonium acrylamide dimethyl taurate / VP copolymer, hydroxyethyl acrylate / sodium acryloyl dimethyl taurate copolymer-squalane-polysorbate-60, and acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer; Preferably, the pH neutralizer comprises any one of aminopropanol or triethanolamine or a combination of both; Preferably, the essence further comprises a preservative; Preferably, the essence further comprises 0.05-1.5% of preservatives by mass percentage; Preferably, the preservative comprises any one of p-hydroxyacetophenone, phenoxyethanol, caprylhydroxamic acid or o-cymene-5-ol, or a combination of at least two thereof.

9. A method for preparing the essence with soothing, repairing and moisturizing effects according to any one of claims 6 to 8, characterized in that: The preparation method comprises the following steps: The substances that need to be dissolved at high temperature among the chelating agent, thickener and moisturizing agent are mixed with water, heated and stirred until completely dissolved, and then the temperature is reduced for the first time, a pH neutralizer and an optional preservative are added, followed by a second temperature reduction, and the remaining components of the skin conditioner and moisturizing agent are added to obtain the essence.

10. The preparation method according to claim 9, characterized in that: The heating is raised to a temperature of 80-85°C; Preferably, the first cooling to a temperature of 50-55°C; Preferably, the second cooling to a temperature is 25-35°C.

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