Application of EGCG (epigallocatechin gallate) as antioxidant health-care component in prevention of cardiovascular diseases

Abstract

The invention relates to the technical field of prevention of cardiovascular diseases, in particular to application of EGCG (epigallocatechin gallate) as an antioxidant health-care component in prevention of cardiovascular diseases, EGCG can neutralize free radicals in a human body and reduce damage of oxidative stress to cells after being absorbed by the human body, and the free radicals are highly active molecules or atomic groups and can attack biomolecules such as cell membranes and DNA (deoxyribonucleic acid), so that the EGCG can be used for preventing cardiovascular diseases. In the prior art, cell injury and dysfunction are caused, the antioxidant and anti-inflammatory effects of EGCG are not isolated, close relation and interaction exist between EGCG and EGCG, and the antioxidant effect can reduce generation and accumulation of free radicals, so that inflammatory response is inhibited; due to the anti-inflammatory effect, damage of inflammation to cells can be relieved, and oxidative stress is further reduced. Due to the synergistic effect, the EGCG shows a more remarkable effect in preventing cardiovascular diseases.

Description

Technical Field

[0001] The present invention relates to the technical field of preventing cardiovascular diseases, and in particular to the application of EGCG as an antioxidant health-care ingredient in preventing cardiovascular diseases. Background Art

[0002] Cardiovascular disease is a type of disease that affects the heart and peripheral blood vessels. It can include arterial stenosis and arterial dilatation. It mainly refers to blood circulation diseases involving the heart and the arteries, veins, and microvessels in the heart. Among them, cardiomyopathy includes dilated cardiomyopathy, hypertrophic cardiomyopathy, arrhythmogenic right ventricular cardiomyopathy, restrictive cardiomyopathy, etc. The main cause is the reconstruction of myocardial tissue. Coronary heart disease can be divided into asymptomatic angina, stable angina, acute coronary syndrome, acute myocardial infarction, ischemic cardiomyopathy, etc. according to the different types of lesions in patients. Hypertension, when blood pressure exceeds 140 / 90mmHg for a long time, it can be diagnosed as hypertension. This is a common cardiovascular disease. Heart valve disease, whether it is the mitral valve, tricuspid valve, pulmonary valve or aortic valve, may cause valve stenosis or insufficiency, which in turn induces heart failure or arrhythmia. Cardiac arrhythmias, including bradyarrhythmias (eg, sick sinus syndrome, atrioventricular block) and tachyarrhythmias (eg, paroxysmal supraventricular tachycardia, premature beats, atrial tachycardia, ventricular tachycardia, atrial fibrillation, and atrial flutter).

[0003] However, cardiovascular diseases are very likely to occur in daily diet in the prior art, and there is a need to solve the problem of preventing cardiovascular diseases and increasing human immunity in the prior art.

[0004] Therefore, those skilled in the art provide the use of EGCG as an antioxidant health ingredient in preventing cardiovascular diseases to solve the problems raised in the above background technology. Summary of the invention

[0005] In order to solve the above technical problems, the present invention provides the application of EGCG, that is, the application of EGCG as an antioxidant health care ingredient in preventing cardiovascular diseases;

[0006] After being absorbed by the human body, the EGCG of the present invention can neutralize free radicals in the body and reduce the damage to cells caused by oxidative stress. Free radicals are highly active molecules or atomic groups that attack biological molecules such as cell membranes and DNA, causing cell damage and dysfunction. In the cardiovascular system, the accumulation of free radicals can promote pathological processes such as atherosclerosis and vascular inflammation;

[0007] Through its antioxidant effect, EGCG can protect cardiovascular cells from oxidative damage and maintain the integrity and function of cell membranes. This helps maintain the elasticity and toughness of blood vessel walls and prevents blood vessel hardening and rupture.

[0008] Lipid peroxidation is an oxidation reaction of low-density lipoprotein (LDL) under the action of free radicals, which is an important step in the formation of atherosclerosis. EGCG can reduce the oxidative modification of LDL, thereby reducing its deposition and accumulation on the blood vessel wall.

[0009] EGCG inhibits inflammatory response. Since cardiovascular disease is often accompanied by inflammatory response, the infiltration of inflammatory cells and the release of inflammatory factors in the blood vessel wall, EGCG can inhibit inflammatory response, reduce the production and release of inflammatory factors, thereby alleviating the inflammatory response of the blood vessel wall, and regulate the function of immune cells. EGCG can also regulate the functions of macrophages, T cells and other immune cells, inhibit their excessive activation and infiltration on the blood vessel wall, and help maintain the normal structure and function of the blood vessel wall. EGCG helps to protect vascular endothelial cells. The blood vessel wall is mainly composed of vascular endothelial cells. When the vascular endothelial cells are affected by dysfunction, it will lead to vascular inflammatory response and decreased vascular function. EGCG can effectively protect vascular endothelial cells from inflammatory damage and maintain their normal physiological functions.

[0010] The antioxidant and anti-inflammatory effects of EGCG do not exist in isolation. There is a close connection and interaction between them. The antioxidant effect can reduce the production and accumulation of free radicals, thereby inhibiting the inflammatory response; while the anti-inflammatory effect can reduce the damage of inflammation to cells and further reduce the occurrence of oxidative stress. This synergistic effect makes EGCG show a more significant effect in preventing cardiovascular diseases. DETAILED DESCRIPTION

[0011] The specific embodiments of the present invention are further described in detail. The examples of the present invention are given for the purpose of illustration and description, and are not intended to be exhaustive or to limit the present invention to the disclosed forms. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiments are selected and described in order to better illustrate the principles and practical applications of the present invention, and to enable those of ordinary skill in the art to understand the present invention and design various embodiments with various modifications suitable for specific uses.

[0012] Example 1: In this example, EGCG is used as an antioxidant health ingredient in the prevention of cardiovascular diseases. After being absorbed by the human body, EGCG can neutralize free radicals in the body and reduce the damage of oxidative stress to cells. Free radicals are highly active molecules or atomic groups that attack biological molecules such as cell membranes and DNA, causing cell damage and dysfunction. In the cardiovascular system, the accumulation of free radicals promotes pathological processes such as atherosclerosis and vascular inflammation;

[0013] Through its antioxidant effect, EGCG can protect cardiovascular cells from oxidative damage and maintain the integrity and function of cell membranes. This helps maintain the elasticity and toughness of blood vessel walls and prevents blood vessel hardening and rupture.

[0014] Lipid peroxidation is an oxidation reaction of low-density lipoprotein (LDL) under the action of free radicals, which is an important step in the formation of atherosclerosis. EGCG can reduce the oxidative modification of LDL, thereby reducing its deposition and accumulation on the blood vessel wall.

[0015] EGCG inhibits inflammatory response. Since cardiovascular disease is often accompanied by inflammatory response, the infiltration of inflammatory cells and the release of inflammatory factors in the blood vessel wall, EGCG can inhibit inflammatory response, reduce the production and release of inflammatory factors, thereby alleviating the inflammatory response of the blood vessel wall, and regulate the function of immune cells. EGCG can also regulate the function of macrophages, T cells and other immune cells, inhibit their excessive activation and infiltration on the blood vessel wall, and help maintain the normal structure and function of the blood vessel wall. EGCG helps to protect vascular endothelial cells. The blood vessel wall is mainly composed of vascular endothelial cells. When the vascular endothelial cells are affected by dysfunction, it will lead to vascular inflammatory response and decreased vascular function. EGCG can effectively protect vascular endothelial cells from inflammatory damage and maintain their normal physiological functions.

[0016] Example 2: This example provides the use of green tea to extract EGCG as an antioxidant health ingredient in preventing cardiovascular diseases;

[0017] EGCG extracted from green tea contains rich polyphenol compounds, including tea polyphenols, catechins and flavonoids. These ingredients have strong antioxidant effects. Antioxidants can help neutralize free radicals, reduce cellular oxidative damage and DNA damage, and thus protect the cardiovascular system. Catechins in green tea are particularly prominent. Their antioxidant properties can significantly reduce the production of free radicals in the body, thereby reducing the risk of arteriosclerosis.

[0018] Polyphenols can reduce the incidence of cardiovascular diseases by inhibiting the release of inflammatory factors and slowing down the damage and repair process of blood vessel walls. Theanine helps lower blood pressure and reduces the increase in blood pressure caused by cardiovascular diseases, thereby further reducing the risk of cardiovascular diseases.

[0019] Example 3: In this example, a method for extracting EGCG is provided for using EGCG as an antioxidant health ingredient in preventing cardiovascular diseases;

[0020] The application of EGCG as an antioxidant health ingredient in the prevention of cardiovascular diseases is carried out according to the following steps of EGCG purification:

[0021] Step 1: Select fresh green tea leaves and place them in a grinder. Continue grinding for 20 to 30 minutes to grind the green tea leaves into fine particles. Then sieve the ground green tea leaves with a mesh size of 20 to 50.

[0022] Step 2: Place the crushed and sieved green tea leaves in step 1 in a container, and soak them in pure water for 30 to 50 minutes. After soaking, extract the EGCG solution twice.

[0023] The EGCG solution was extracted once by heating the vessel to 85-90°C, with a material-liquid ratio of 1:8, and the heating extraction time was 30-40 min;

[0024] For secondary extraction, the EGCG solution was heated to 65-80°C in a vessel, and the material-liquid ratio was 1:4, and the heating time was 17-25 min to obtain the EGCG extract;

[0025] Step 3: The EGCG extract obtained in step 2 is subjected to an ultrafiltration membrane to extract the liquid, and the impurities remaining in the EGCG extract are filtered out;

[0026] The filtered EGCG extract is placed in an evaporation concentrator to evaporate the liquid at high temperature to increase the concentration of EGCG and obtain an EGCG concentrated solution;

[0027] Step 4: The EGCG concentrate obtained in step 3 is subjected to adsorption separation using D-101 perforated adsorption resin to remove impurities and enrich EGCG. The treated EGCG concentrate passes through an adsorption column filled with resin. The resin selectively adsorbs EGCG molecules in the solution through its porous structure, while most impurities are not adsorbed or adsorbed weakly, thereby achieving preliminary enrichment of EGCG.

[0028] Then, the adsorption column is rinsed with pure water solvent to remove impurities adsorbed on the resin but not the target components, thereby improving the purity of EGCG. Then, ethanol is used as a desorbent to desorb EGCG from the resin by changing the solvent, and the desorbed liquid is collected to obtain an EGCG enriched solution.

[0029] Step 5: placing the EGCG enriched solution obtained in step 4 into a crystallization furnace for crystallization processing;

[0030] Step six: drying the EGCG crystals crystallized in step five, crushing and sieving the dried EGCG crystals to obtain the EGCG for cardiovascular disease, an antioxidant health ingredient.

[0031] Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field and related fields without creative work should fall within the scope of protection of the present invention. The structures, devices and operating methods not specifically described and explained in the present invention are implemented according to the conventional means in the field unless otherwise specified and limited.

Claims

1. The application of EGCG as an antioxidant health ingredient in preventing cardiovascular diseases is characterized by The application of EGCG as an antioxidant health ingredient in preventing cardiovascular diseases; The application of EGCG as an antioxidant health ingredient in the prevention of cardiovascular diseases is carried out according to the following steps of EGCG purification: Step 1: Select fresh green tea leaves and place them in a grinder. Continue grinding for 20 to 30 minutes to grind the green tea leaves into fine particles. Then sieve the ground green tea leaves with a mesh size of 20 to 50. Step 2: Place the crushed and sieved green tea leaves in step 1 in a container, and soak them in pure water for 30 to 50 minutes. After soaking, extract the EGCG solution twice. The EGCG solution was extracted once by heating the vessel to 85-90°C, with a material-liquid ratio of 1:8, and the heating extraction time was 30-40 min; For secondary extraction, the EGCG solution was heated to 65-80°C in a vessel, and the material-liquid ratio was 1:4, and the heating time was 17-25 min to obtain the EGCG extract; Step 3: The EGCG extract obtained in step 2 is subjected to an ultrafiltration membrane to extract the liquid, and the impurities remaining in the EGCG extract are filtered out; The filtered EGCG extract is placed in an evaporation concentrator to evaporate the liquid at high temperature to increase the concentration of EGCG and obtain an EGCG concentrated solution; Step 4: The EGCG concentrate obtained in step 3 is subjected to adsorption separation using D-101 perforated adsorption resin to remove impurities and enrich EGCG. The treated EGCG concentrate passes through an adsorption column filled with resin. The resin selectively adsorbs EGCG molecules in the solution through its porous structure to achieve preliminary enrichment of EGCG. Then, the adsorption column is rinsed with pure water solvent to remove impurities adsorbed on the resin but not the target components, thereby improving the purity of EGCG. Then, ethanol is used as a desorbent to desorb EGCG from the resin by changing the solvent, and the desorbed liquid is collected to obtain an EGCG enriched solution. Step 5: placing the EGCG enriched solution obtained in step 4 into a crystallization furnace for crystallization processing; Step six: drying the EGCG crystals crystallized in step five, crushing and sieving the dried EGCG crystals to obtain the EGCG for cardiovascular disease, an antioxidant health ingredient.