Anti-radiation traditional Chinese medicine composition as well as preparation method and application thereof

Abstract

The invention discloses an anti-radiation traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from 10 parts by weight of a gardenia jasminoides ellis extracting solution and 1 part by weight of a 0.02 g / 100mL vitamin C solution. The research proves that the traditional Chinese medicine composition containing the fructus gardeniae extract and the vitamin C can effectively reduce the death rate caused by radiation, and the survival time of mice after radiation is well prolonged on the premise of ensuring certain life quality. In addition, the traditional Chinese medicine composition can effectively reduce the micronucleus formation rate in bone marrow cells and promote the recovery of a bone marrow hematopoietic system. The invention provides a method for protecting healthy people from ionizing radiation injury by using the traditional Chinese medicine composition, and the traditional Chinese medicine composition can be used for resisting radiation, improving the lifetime and protecting the bone marrow hematopoiesis function, and has important significance on improving the survival rate after radiation and reducing the death rate.

Description

Technical Field

[0001] The invention relates to a Chinese medicine composition, in particular to an anti-radiation Chinese medicine composition containing Gardenia jasminoides component and its application. Background Art

[0002] After ionizing radiation acts on the body, through complex physical, chemical and biological mechanisms, first macromolecules such as DNA are damaged, and then cells, tissues, organs, and even systems and the entire body's functional metabolism and morphology are damaged. The harm of radiation to the body affects many important systems that maintain normal life activities of the human body, such as hematopoiesis, immunity, and central nervous system. Among them, hematopoietic tissue is a highly radiation-sensitive tissue. After the body is irradiated, the blood system quickly undergoes very obvious changes in morphology, function, quantity, and characteristics. After the body's cells are exposed to ionizing radiation, a large amount of reactive oxygen is produced, causing oxidative stress, leading to DNA mutations, cell apoptosis, cell cycle arrest, etc., causing oxidative damage to cells and reduced vitality.

[0003] With the in-depth development of modern atomic energy technology, more and more workers are facing health risks caused by accidental exposure to ionizing radiation. Improving the ability of radiation-related workers to resist ionizing radiation is an urgent research goal. Researchers have conducted a lot of exploration work on radiation protection equipment and anti-radiation drugs. However, protective equipment such as protective clothing can only provide external protection. Existing synthetic protective agents mainly include thiol compounds, nitroxide free radicals, dibenzimidazole, etc., which mainly focus on scavenging free radicals, inhibiting cell metabolism and promoting damaged cell repair, and less on the protective effect on the bone marrow hematopoietic system. Moreover, most of these protective agents have the disadvantages of high price, large application dose, strong toxic side effects, etc. Therefore, it is very meaningful to develop new protective agents with high efficiency, low toxicity, low cost and multiple targets. Many natural Chinese medicines meet the above requirements. Chinese medicine compound ingredients are rich and can play the role of resisting adverse environments through multiple targets and pathways. Modern pharmacological studies have shown that some single Chinese medicines have protective effects on hematopoietic cells and hematopoietic tissues, and some single Chinese medicines can promote human cell repair, restore and ensure the function of cell functions.

[0004] In traditional Chinese medicine, Wen Zhizi is called "Gardenia" or "Shan Zhi". It tastes bitter and is cold in nature. It enters the heart, lung and triple burner meridians. The lung runs through the heart meridian to circulate qi and blood. If the heart qi is insufficient, blood circulation will be blocked. The upper heart, the middle spleen and stomach, and the lower liver and kidneys. The triple burner is the "general manager of the five internal organs and six bowels" and can communicate the airway, waterway and bloodway. The main components of Wen Zhizi include iridoid glycosides, flavonoids, phenylethanol glycosides, triterpenoids, organic acids, essential oils, etc., which have multiple effects such as anti-inflammatory, antioxidant, antiviral, anti-tumor and liver protection. The dried and mature fruits of Gardenia jasminoides are used as medicine, which has the effects of protecting the liver and promoting bile secretion, purging fire and relieving restlessness, cooling blood and detoxifying, clearing away heat and dampness, and is used for heat pathogenic heat invading the heart, chest distress, restlessness and insomnia, bladder heat due to stagnation of heat and dampness, jaundice, fever, short and red urine caused by stagnation of heat and dampness in the liver and gallbladder, and blood-stained stranguria, dribbling and painful urination, vomiting and bleeding due to blood heat, and red and swollen eyes caused by stagnation of heat in the bladder. Mashing raw Gardenia jasminoides for external application has the effect of clearing heat, reducing swelling and relieving pain, and can be used for sprains and contusions.

[0005] Therefore, the development of highly effective, low-toxic, and simple-to-prepare radiation-proof Chinese herbal compositions is of great significance for protecting against ionizing radiation, especially in repairing damage to the bone marrow hematopoietic system caused by ionizing radiation. Summary of the invention

[0006] The purpose of the present invention is to provide an anti-radiation Chinese medicine composition and a preparation method and application thereof. The anti-radiation Chinese medicine composition can effectively protect the hematopoietic function of the bone marrow and repair the damage caused by radiation, thereby achieving an effective anti-radiation effect.

[0007] In a first aspect, the present invention provides an anti-radiation Chinese medicine composition, which comprises a Gardenia jasminoides extract and vitamin C, wherein the weight proportions of the raw materials are as follows:

[0008] Gardenia jasminoides extract: 10 parts by weight;

[0009] 0.02g / 100mL Vitamin C solution: 1 part by weight.

[0010] In the second aspect, the present invention also discloses a method for preparing the above-mentioned radiation-resistant Chinese medicine composition, which should also fall within the protection scope of the present invention. The preparation method specifically comprises the following steps:

[0011] According to the prescription, 4.0g of warm gardenia medicinal materials are taken, chopped and soaked in 200mL ultrapure water for 30 minutes, decocted for the first time with high heat for 30 minutes, and the upper layer of liquid is poured out, i.e., the first decoction; 150mL of water is added to continue decocting for 30 minutes, the upper layer of liquid is poured out, the gauze is wrapped with the residue for filtering, and the second decoction is obtained. The first decoction and the second decoction are mixed, the liquid is cooled and centrifuged at 4500rPM for 15 minutes, the supernatant is taken, and filtered with three layers of gauze, and then stored in a refrigerator at 4°C to obtain the warm gardenia extract. When used, 0.02g / 100mL of vitamin C solution of corresponding weight parts is added and mixed to obtain the radiation-resistant Chinese medicine composition of the present invention.

[0012] The application of the above-mentioned anti-radiation Chinese medicine composition and its main components in combination in medicines and health care products should also fall within the protection scope of the present invention.

[0013] The present invention also provides an anti-radiation Chinese medicine compound preparation, which specifically comprises the above-mentioned anti-radiation Chinese medicine composition, prepared into a preparation according to a conventional method.

[0014] The advantages of the radiation-resistant Chinese medicine composition preparation provided by the present invention are as follows:

[0015] 1. The radiation-resistant Chinese medicine composition provided by the present invention can effectively protect the hematopoietic function of the bone marrow, repair the damage caused by radiation, and improve the quality of life, thereby achieving an effective radiation-resistant effect.

[0016] 2. The radiation-resistant Chinese medicine composition of the present invention has a precise radiation-resistant effect, low toxicity and side effects, a simple preparation method, low cost, and is easy to take, and has good prospects in preventing and treating radiation damage. DETAILED DESCRIPTION

[0017] The following are specific embodiments of the present invention, which further describe the technical solution of the present invention, but the protection scope of the present invention is not limited to these embodiments. Any changes or equivalent substitutions that do not deviate from the concept of the present invention are included in the protection scope of the present invention.

[0018] The experimental methods used in the following examples are conventional methods unless otherwise specified. All the parts involved are by weight unless otherwise specified. The preparation prepared from the radiation-resistant Chinese medicine composition of the present invention is a liquid obtained from pharmaceutically acceptable excipients, which is a decoction after the raw materials are processed, decocted and filtered.

[0019] Example 1

[0020] The present invention provides an anti-radiation Chinese medicine composition, wherein the weight proportions of the raw materials are as follows:

[0021]

[0022] The preparation method of the radiation-resistant Chinese medicine composition is as follows:

[0023] According to the prescription, 4.0g of warm gardenia medicinal materials are taken, chopped and soaked in 200mL ultrapure water for 30 minutes, decocted for the first time with high heat for 30 minutes, and the upper layer of liquid is poured out, i.e., the first decoction; 150mL of water is added to continue decocting for 30 minutes, the upper layer of liquid is poured out, the gauze is wrapped with the residue for filtering, and the second decoction is obtained. The first decoction and the second decoction are mixed, the liquid is cooled and centrifuged at 4500rPM for 15 minutes, the supernatant is taken, and filtered with three layers of gauze, and then stored in a refrigerator at 4°C to obtain the warm gardenia extract. When used, 0.02g / 100mL of vitamin C solution of corresponding weight parts is added and mixed to obtain the radiation-resistant Chinese medicine composition of the present invention.

[0024] Example 2 Effect of the Chinese medicine composition of the present invention on the mortality rate and body weight change of mice

[0025] In order to study the anti-radiation effect of the anti-radiation Chinese medicine composition of the present invention on survival conditions, a study was conducted.

[0026] 1. Experimental Methods

[0027] 1.1 Animal husbandry and grouping

[0028] 1.1.1 Experimental conditions

[0029] Forty 6-8 week old SPF grade ICR male mice weighing 30±2 g each were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. and were raised under standard experimental conditions of free access to food and water at a temperature of 20±2°C, a humidity of 80±5% and a pressure of 90.0 kpa. The mice were randomly divided into 4 groups, with 10 mice in each group.

[0030] 1.1.2 Group Processing

[0031] According to the principles of Experimental Pharmacology Methodology, the dosing levels for the experimental group and the positive control group were calculated according to the clinical dosage for adults, and the radiation protection drugs for the following treatments were prepared.

[0032] After one week of adaptation, mice were gavaged once a day for one week before the irradiation experiment, and whole-body irradiation was performed 30 minutes after the last administration. During irradiation, mice were fixed in resin cages with air holes (or fixed in special plexiglass boxes), and animals were irradiated with 7.0Gy of Se137-γ rays using HXFS-IA blood irradiator at the Southern Zhejiang Institute of Radiation Medicine and Nuclear Technology. Mice in the blank control group were operated in the same way but not irradiated.

[0033] Treatment 1: The Chinese medicine composition for radiation protection of the present invention comprises 10 parts by weight of warm gardenia extract; 1 part by weight of 0.02g / 100mL vitamin C solution. Take 4.0g of warm gardenia medicinal material according to the prescription amount, chop it and soak it in 200mL ultrapure water for 30 minutes, decoct it for the first time with high heat for 30 minutes, pour out the upper layer of liquid, i.e. the first decoction; add 150mL of water and continue to decoct for 30 minutes, pour out the upper layer of liquid, wrap the residue with gauze to filter the residue, and combine to obtain the second decoction. The first decoction and the second decoction are mixed, and the liquid is cooled and centrifuged at 4500rPM for 15 minutes, the supernatant is taken, and filtered with three layers of gauze, and then stored in a refrigerator at 4°C to obtain the warm gardenia extract. When using, add the corresponding weight portion of 0.02g / 100mL vitamin C solution and mix well to obtain the radiation-resistant Chinese medicine composition of the present invention.

[0034] Treatment 2: Use Curcuma wenyujin to replace the Gardenia wenyujin in Example 1, and its weight is 55 weight parts of Curcuma wenyujin; 1 weight part of 0.02g / 100mL vitamin C solution. Take 21.875g of Curcuma wenyujin medicinal materials according to the prescription amount, chop them and soak them in 200mL ultrapure water for 30 minutes, decoct them for the first time with high heat for 15 minutes, pour out the upper layer of liquid, that is, the first decoction; add 150mL of water and continue to decoct for 15 minutes, pour out the upper layer of liquid, wrap the residue with gauze to filter the residue, and combine to obtain the second decoction. The first decoction and the second decoction are mixed, and the liquid is cooled and centrifuged at 4500rPM for 15 minutes, the supernatant is taken, and filtered with three layers of gauze, and then stored in a refrigerator at 4°C. When used, add the corresponding weight parts of 0.02g / 100mL vitamin C solution and mix well. The obtained Chinese medicine composition is recorded as the Curcuma wenyujin + vitamin C group.

[0035] Treatment 3: Use honeysuckle to replace the warm gardenia in Example 1, and its weight parts are 17 weight parts of honeysuckle; 1 weight part of 0.02g / 100mL vitamin C solution. Take 6.7g of honeysuckle medicinal materials according to the prescription amount, chop them and soak them in 200mL ultrapure water for 30 minutes, decoct them for the first time with high heat for 15 minutes, pour out the upper layer of liquid, that is, the first decoction; add 150mL of water and continue to decoct for 15 minutes, pour out the upper layer of liquid, wrap the residue with gauze to filter the residue, and combine to obtain the second decoction. The first decoction and the second decoction are mixed, and the liquid is cooled and centrifuged at 4500rPM for 15 minutes, the supernatant is taken, and filtered with three layers of gauze, and then placed in a 4=C refrigerator for storage. When used, add the corresponding 1 weight part of 0.02g / 100mL vitamin C solution and mix well. The obtained Chinese medicine composition is recorded as honeysuckle + vitamin C group.

[0036] Treatment 4: 0.02 g / 100 mL vitamin C solution was used as the radiation protection drug, recorded as the vitamin C group.

[0037] Treatment 5: an equal amount of distilled water, recorded as the radiation control group.

[0038] Treatment 6: equal amount of distilled water, no irradiation, other treatments were the same as treatments 1-5, recorded as blank control group.

[0039] 2. Experimental results

[0040] After 15 days of treatment with the Chinese medicine composition of the present invention, the survival rate of mice and the changes in body weight with prolonged irradiation time are shown in Table 1-2 below.

[0041] Table 1 15-day survival rate of mice

[0042]

[0043] As shown in Table 1, the anti-radiation Chinese medicine composition of the present invention can effectively reduce the mortality of acutely irradiated mice, better prolong the survival time of mice after radiation, and improve the survival rate of mice after radiation.

[0044] Table 2 Changes in mouse body weight with prolonged irradiation time

[0045]

[0046] It can be seen from Table 2 that the radiation-resistant Chinese medicine composition of the present invention can effectively alleviate the weight loss of mice after irradiation and can improve the quality of life of mice to a certain extent.

[0047] Example 3 Effect of the Chinese medicine composition of the present invention on the bone marrow hematopoietic function of mice

[0048] In order to study the protective effect of the radiation-resistant Chinese medicinal composition of the present invention on bone marrow hematopoietic function, a study was conducted.

[0049] 1. Experimental Methods

[0050] The feeding and grouping of experimental animals were the same as in Example 2.

[0051] 1.1 Blood and bone marrow collection

[0052] 15 days after the experimental animals were irradiated, their eyeballs were removed and blood was collected using blood collection tubes and then processed and stored as soon as possible according to the standard blood sample processing procedures.

[0053] Kill the mice by spinal cord injury, then cut the skin and muscles on the thigh with scissors, remove the thigh bone, and use a small piece of gauze to rub the muscle debris attached to the bone. Cut off the enlarged joint heads at both ends of the femur, then use a 2mL syringe to absorb 2mL of Hanks solution, insert it into one end of the femur, and flush the bone marrow cells into a 2mL centrifuge tube. Repeat the flushing several times until the bone marrow cavity turns white.

[0054] 1.2 Centrifugation

[0055] The obtained cell suspension was centrifuged at 1000 rpm for 10 minutes, the supernatant was removed by aspiration, and 2 drops of inactivated calf serum were added to the precipitate to prepare a cell suspension.

[0056] 1.3 Smear

[0057] Place a drop of the suspension on one end of the slide, smear it in the usual way, and let it dry in the air.

[0058] 1.4 Fixed

[0059] Place the dried slides in methanol fixative for 10 minutes and take them out to dry.

[0060] 1.5 Dyeing

[0061] Place the slide flat on a glass plate, stain with Giemsa for 15 minutes, rinse with running water, dry and then examine under a microscope.

[0062] 1.6 Observation

[0063] First observe under a low power microscope, select the area with even distribution and good staining, and then observe and count under an oil microscope. Polychromatic erythrocytes (PCE) are gray-blue, and orthochromatic erythrocytes (NCE) are orange-yellow. Most of the micronuclei contained in the cells are round, with smooth and neat edges, and the color is consistent with the nucleoplasm, which is purple-red or blue-purple.

[0064] 1.7 Count

[0065] One or more micronuclei may appear in a cell. Count the number of PCEs containing micronuclei among 2000 PCEs, calculate the micronucleus cell rate, and observe and record the results.

[0066] 2. Experimental results

[0067] After 15 days of treatment with the Chinese medicine composition of the present invention, the changes in the blood routine and micronucleus formation rate of mice are shown in the following Tables 3-5.

[0068] Table 3 Statistics of blood routine indicators

[0069]

[0070] Table 4 Comparison of blood routine indicators

[0071]

[0072] Radiation damage to the bone marrow inhibits the generation of white blood cells, red blood cells and platelets. As shown in Tables 3-4, the white blood cell, red blood cell and platelet counts of each group of mice after irradiation were significantly reduced compared with the blank control group, and the difference was statistically significant. Compared with the radiation control group, the anti-radiation Chinese medicine composition of the present invention increased the white blood cell, red blood cell and platelet counts, but the difference was not statistically significant.

[0073] Table 5 Statistics of micronucleus formation rate

[0074]

[0075] Table 6 Comparison of micronucleus formation rate

[0076]

[0077] Further micronucleus test results (Table 5-6) show that the radiation-resistant Chinese medicine composition of the present invention can effectively reduce the micronucleus formation rate in bone marrow cells and has the effect of protecting bone marrow hematopoietic function.

[0078] In summary, the radiation protection Chinese medicine composition of the present invention has a significant effect in saving the survival rate of acute radiation damage and shows a protective effect in the bone marrow hematopoietic system.

[0079] The above embodiments are only used to illustrate the technical solutions of the present invention, but not to limit the technical solutions. Any technical solutions within the scope of the present invention or any improvements and modifications that do not deviate from the principle of the present invention shall fall within the protection scope of the present invention.

Claims

1. A radiation-resistant Chinese medicine composition, characterized in that: The traditional Chinese medicine composition comprises gardenia jasminoides extract and vitamin C.

2. The Chinese medicine composition according to claim 1, characterized in that: The invention comprises the following raw materials in parts by weight: 10 parts by weight of warm gardenia extract; and 1 part by weight of 0.02g / 100mL vitamin C solution.

3. The Chinese medicine composition according to claim 1, characterized in that The traditional Chinese medicine composition can effectively protect the hematopoietic function of the bone marrow and repair the damage caused by radiation.

4. A method for preparing a radiation-resistant Chinese medicine composition according to any one of claims 1 to 3, characterized in that: The following steps are involved: According to the prescription, 4.0g of warm gardenia medicinal materials are taken, chopped and soaked in 200mL ultrapure water for 30 minutes, decocted for the first time with high heat for 30 minutes, and the upper layer of liquid is poured out, i.e., the first decoction; 150mL of water is added to continue decocting for 30 minutes, the upper layer of liquid is poured out, the gauze is wrapped with the residue for filtering, and the second decoction is obtained. The first decoction and the second decoction are mixed, the liquid is cooled and centrifuged at 4500rPM for 15 minutes, the supernatant is taken, and filtered with three layers of gauze, and then stored in a refrigerator at 4°C to obtain the warm gardenia extract. When used, 0.02g / 100mL of vitamin C solution of corresponding weight parts is added and mixed to obtain the radiation-resistant Chinese medicine composition of the present invention.

5. The Chinese medicine composition according to claims 1-3, characterized in that: The traditional Chinese medicine composition further includes pharmaceutically acceptable excipients.

6. The use of the radiation-resistant Chinese medicine composition according to any one of claims 1 to 3, characterized in that: Application of the Chinese medicine composition in radiation-resistant medicines or health products.

7. A radiation-resistant Chinese medicinal compound preparation, characterized in that: The invention comprises the radiation-resistant Chinese medicine composition according to any one of claims 1 to 3 and auxiliary materials, and is prepared into a preparation according to a conventional method.

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