Rapid detection kit for swine fever antibody

By using recombinant proteins and multiple sets of disposable reservoirs and storage frames in the swine fever antibody rapid detection kit, combining photochromic balls and hygroscopic cotton to determine the storage environment, the detection problem caused by improper storage of the kit is solved. Improves detection accuracy and convenience, and enhances protection effect.

CN119284333AInactive Publication Date: 2025-01-10BEIJING WANBO BAICHI BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202411659264.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-11-20
Publication Date
2025-01-10
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

The existing swine fever antibody rapid detection kits can easily lead to ineffective reagents when stored incorrectly, affecting the accuracy of the detection results, and have low detection accuracy and poor protection effect.

Method used

A swine fever antibody rapid detection kit was designed, using recombinant proteins P54 and P72 proteins for detection, with chimeric sockets on the top, card slots and storage plates were installed on both sides of the inner walls, and limit holes and slots were arranged on the storage plates. , a limit block is embedded in the limit hole. A barrier cover and a light-sensitive color-changing ball are installed on the limit block. The barrier cover is embedded with moisture-absorbing cotton. The reagent storage environment is judged through the light-sensitive color-changing ball and moisture-absorbing cotton, and multiple sets of disposable liquid storage tubes are set up. With the storage box, the storage box separates the space to place the test tube and test paper, and the limit box is fixed by magnets and iron blocks to prevent falling.

Benefits of technology

It improves the accuracy and convenience of the detection, avoids the problem of reagent ineffectiveness caused by improper storage, enhances the functionality and protective effect of the kit, and ensures the accuracy and flexibility of the test results.

✦ Generated by Eureka AI based on patent content.

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Abstract

The present invention discloses a swine fever antibody rapid detection kit, and relates to the technical field of kits, the swine fever antibody rapid detection kit comprises a kit body, the top of the kit body is sleeved with a top cover in an embedded manner, and the inner walls of the two sides of the kit body are provided with clamping grooves; a storage plate is mounted in the clamping groove in an embedded mode, limiting holes and first inserting grooves which are evenly distributed are formed in the top of the storage plate in a penetrating mode, the limiting holes are located in the two sides of the first inserting grooves, limiting blocks are mounted in the limiting holes in an embedded mode, blocking covers are mounted at the tops of the limiting blocks, and light sensation color changing balls and moisture absorption cotton which are evenly distributed are mounted on the inner side walls of the blocking covers. The moisture absorption cotton is located below the light sensation color changing ball. The outer surface of the light-sensitive color-changing ball is sprayed with a light-sensitive material used for detecting the shading effect of the blocking cover. When the kit is used, the storage environment condition of a monitored reagent in the kit is judged by observing the color change condition of the light sensation color change ball in the blocking cover and the touch sense of the moisture absorption cotton, and the condition that an invalid reagent appears due to improper storage is avoided.
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Description

Technical Field

[0001] The invention relates to the technical field of kits, in particular to a rapid detection kit for swine fever antibodies. Background Art

[0002] The Classical Swine Fever Antibody Rapid Detection Kit is a tool used to quickly detect the level of Classical Swine Fever antibodies in pigs. By detecting Classical Swine Fever antibodies, we can understand the immune status of the pig herd, evaluate the immune effect of the vaccine, and provide an important basis for the prevention and control of Classical Swine Fever. However, the reagents in the existing detection kits are prone to become invalid due to improper storage, which in turn affects the test results, and there are certain defects.

[0003] The defects of existing detection kits are: 1. In the patent document CN212244595U, the dust removal effect in the test kit box is mainly improved by a ventilation pipe provided with a filter, but it does not take into account that the reagents in the existing test kits are easily invalidated due to improper storage, thereby affecting the test results; 2. In the patent document CN209764880U, four areas are set, each of which can be marked with a label. When conducting a swine fever antibody detection experiment, each antibody detection reagent can be quickly distinguished, which is convenient for distinguishing and classifying antibodies. It does not take into account that the detection reagents in the existing detection kits are easily stored after use, which may affect the effect of subsequent detection; 3. In the patent document CN216233634U, reagent bottles are stored in multiple placement slots that can provide fluorescence and color prompts, and a light source is provided by a light-transmitting extrusion structure, so that the reagent bottles can be viewed and distinguished in the test kit, which is convenient for distinguishing different reagent bottles and reduces the possibility of damage to the reagent bottles. It does not take into account that the accuracy of existing detection kits is low and the test results are easily affected by other antibodies; 4. In the patent document CN111929433B, antibody positivity can be detected earlier at a lower antibody level in the early stage, providing a basis for the clinical pig herd infection situation, but it does not take into account the problem that the existing detection kit has a poor protective effect when used. Summary of the invention

[0004] The object of the present invention is to provide a rapid detection kit for classical swine fever antibodies to solve the problems raised in the above-mentioned background technology.

[0005] To achieve the above object, the present invention provides the following technical solutions: a rapid detection kit for classical swine fever antibodies, comprising a kit, wherein the kit uses recombinant proteins P54 and P72 proteins as detection antigens to detect classical swine fever antibodies; The top of the reagent box is fitted with a top cover, and both inner walls of the two sides of the reagent box are provided with card slots, and a storage plate is fitted inside the card slots, and the top of the storage plate is penetrated with evenly arranged limiting holes and slot one, and the limiting holes are located on both sides of the slot one, and the limiting holes are fitted inside the limiting holes. A blocking cover is installed on the top of the limiting block, and the size of the blocking cover is larger than the size of the slot one. Evenly arranged light-sensitive color-changing balls and moisture-absorbing cotton are installed on the inner side wall of the blocking cover, and the moisture-absorbing cotton is located below the light-sensitive color-changing balls; The outer surface of the light-sensitive color-changing ball is sprayed with light-sensitive material for detecting the light-shielding effect of the shielding cover.

[0006] Preferably, a sponge block is embedded and installed inside the test kit, the sponge block is located below the storage plate, and the top of the sponge block is provided with evenly arranged slots two, and slots one and two are each provided with equidistantly arranged plug plates, and the tops of multiple groups of plug plates are connected to disposable liquid storage tubes, and the disposable liquid storage tubes are located on the inner side of the baffle, and the outer surfaces of the multiple groups of disposable liquid storage tubes are provided with connecting plates, and the connecting plates between every two disposable liquid storage tubes can be rotatably separated, and the interior of each group of disposable liquid storage tubes is respectively filled with reagents required for swine fever antibody detection.

[0007] Preferably, storage slots are provided on both side outer walls of the reagent box, a storage frame is slidably placed inside one group of the storage slots, and the bottom of the storage frame is in contact with the bottom wall of the reagent box, a partition is provided inside the storage frame, the partition is used to divide the storage frame into two equal spaces, a storage frame and a partition frame are installed on the bottom wall of the storage frame, the partition frame is located on one side of the storage frame, disposable test tubes are evenly arranged inside the storage frame, a sealing cover is threadedly connected to the top of the disposable test tube, and the disposable test tubes are detachably connected to each other, a disposable pre-coated test plate is placed inside the partition frame, and disposable test strips are evenly arranged inside the storage frame, and the partition is located between the partition frame and the disposable test strip.

[0008] Preferably, the outer surfaces of the test kit and the top cover are provided with symmetrically arranged limit boxes, and the surfaces of the two groups of limit boxes close to each other are installed with limit plates, and the inner surfaces of the two groups of limit plates are respectively fitted with the outer surfaces of the top cover and the test kit, one side outer wall of one group of limit plates is embedded with a magnet, and one side outer wall of the other group of limit plates is embedded with an iron block, and one side outer wall of the iron block is fitted with one side outer wall of the magnet.

[0009] Preferably, another group of the storage slots has storage blocks slidably placed inside, and the tops of the storage blocks are provided with storage holes with different apertures, and the storage holes are used to place disposable test tubes of different sizes.

[0010] Preferably, a drawstring is installed on one side outer wall of the storage frame and the storage block, and the drawstring is used to pull the storage frame and the storage block out of the storage slot on the reagent box.

[0011] Preferably, a slot is provided through the top of the storage plate, and the slot is used to take the storage plate out of the card slot and the reagent box.

[0012] Preferably, a sponge pad is installed on the top wall of the top cover, and the bottom of the sponge pad fits with the top of the baffle.

[0013] Preferably, the method of using the detection kit is as follows: S1. First, pull the limit box to both sides so that the magnet is no longer attracted to the iron block, and then the limit box can be removed from the reagent box and the top cover; S2, then pull the storage frame outwards to take out the disposable test tubes, disposable pre-coated test plates and disposable test strips required for the test; S3, then pull the shield upwards so that the limit block is no longer engaged with the limit hole, and then take out a set of disposable liquid storage tubes placed in slot one; S4. Finally, a set of disposable liquid storage tubes, disposable pre-coated test plates and disposable test strips are used to perform multiple swine fever antibody tests on the collected samples.

[0014] Preferably, in S3, it also includes: after removing the shield, immediately observing the color change of the light-sensitive color-changing ball inside and the touch of the moisture-absorbing cotton, so as to judge the preservation condition of the reagent in the disposable liquid storage tube inside the shield.

[0015] Compared with the prior art, the present invention has the following beneficial effects: 1. When using the test kit of the present invention, first open the top cover, then pull the blocking cover upwards so that the limit block is no longer engaged with the limit hole, then observe the color change of the photosensitive material on the surface of the photochromic ball in the blocking cover. If there is no change, it means that the light-shielding property of the blocking cover is good, otherwise it means that there is a defect and the light-shielding property is poor. Then touch the hygroscopic cotton and feel its touch to judge the storage environment of the reagents in the blocking cover in the test kit to avoid the occurrence of invalid reagents due to improper storage, thereby improving the accuracy of the detection to a certain extent.

[0016] 2. The present invention provides multiple groups of disposable liquid storage tubes, each group has multiple single disposable liquid storage tubes, and they are connected by a tearable connecting plate. Each group of different disposable liquid storage tubes contains different amounts of detection reagents. When in use, only one group of disposable liquid storage tubes needs to be taken out to complete a single test, thereby improving the convenience of the test and avoiding the situation where the subsequent test results are affected by improper storage of the reagents after use. To a certain extent, the convenience of swine fever antibody detection and the practicality of the test kit can be improved.

[0017] 3. The present invention improves the functionality of the test kit by arranging a storage frame and a storage block in the test kit. The storage frame is divided into storage spaces by partitions, half of which is used to place well-packaged disposable test strips, and the other half is used to place disposable pre-packaged test plates packaged in disposable test tube boxes. The disposable test tubes are also designed to be separable, and the number of disposable test tubes can be selected according to usage needs, thereby improving flexibility during detection. The disposable pre-packaged test plates and disposable test strips are designed to compare two or more groups of test results during a single test, thereby improving the detection accuracy while reducing or distinguishing CSFV-specific antibodies, BVDV, BDV, and antibodies produced by other ruminant pestivirus infections. In addition, the storage block is provided for placing disposable test tubes of different sizes during detection, thereby improving the functionality of the test kit and providing a storage platform during detection.

[0018] 4. When the reagent kit is not needed, the present invention covers the top cover on the reagent kit, and then puts two sets of limit boxes on both sides of the reagent kit and the top cover, and pushes the limit boxes toward the middle, and then the limit boxes are firmly fixed on the reagent kit and the top cover by the attraction of the magnet and the iron block on the limit plate, thereby preventing the items in the reagent kit from being spilled and damaged due to accidental contact and falling, thereby improving the protection of the reagent kit. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 It is a schematic diagram of the assembly structure of the test kit and the top cover of the present invention; Figure 3 It is a schematic diagram of the assembly structure of the storage plate and the shield of the present invention; Figure 4 It is a schematic diagram of the assembly structure of the storage plate and the reagent kit of the present invention; Figure 5 It is a schematic diagram of the planar assembly structure of the limiting box and the reagent kit of the present invention; Figure 6 It is a schematic diagram of the planar assembly structure of the baffle cover and the disposable liquid storage tube of the present invention; Figure 7 It is a schematic diagram of the plane assembly structure of the storage frame of the present invention; Figure 8 It is a schematic diagram of the planar assembly structure of the disposable test tube of the present invention; Fig. 9 It is a schematic diagram of the assembly structure of the storage block and the reagent kit of the present invention.

[0020] In the figure: 1. reagent box; 2. limit box; 3. top cover; 4. card slot; 5. storage plate; 6. slot hole; 7. limit hole; 8. slot one; 9. limit block; 10. shield; 11. light-sensitive color-changing ball; 12. moisture-absorbing cotton; 13. sponge block; 14. slot two; 15. plug-in board; 16. disposable liquid storage tube; 17. connecting plate; 18. sponge pad; 19. storage frame; 20. partition; 21. storage frame; 22. disposable test tube; 23. sealing cover; 24. partition frame; 25. disposable test paper; 26. storage block; 27. placement hole; 28. limit plate; 29. ​​magnet; 30. iron block. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0022] In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "front end", "rear end", "two ends", "one end", "the other end" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance.

[0023] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "provided with", "connected", etc. should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0024] See also Figure 1 , Figure 2 , Figure 3 , Figure 4 and Figure 6An embodiment of the present invention is a rapid detection kit for swine fever antibodies, comprising a kit 1, wherein the kit 1 uses recombinant proteins P54 and P72 as detection antigens to detect swine fever antibodies, wherein a top cover 3 is embedded and sleeved on the top of the kit 1, and both sides of the inner wall of the kit 1 are provided with card slots 4, and a storage plate 5 is embedded and installed inside the card slots 4, and the top of the storage plate 5 is penetrated by uniformly arranged limiting holes 7 and slots 8, wherein the limiting holes 7 are located on both sides of the slots 8, and limiting blocks 9 are embedded and installed inside the limiting holes 7, and the limiting blocks 9 A blocking cover 10 is installed on the top, and the size of the blocking cover 10 is larger than that of the slot 8. The inner wall of the blocking cover 10 is installed with evenly arranged photochromic balls 11 and moisture-absorbing cotton 12. The moisture-absorbing cotton 12 is located below the photochromic balls 11. The outer surface of the photochromic balls 11 is sprayed with photosensitive material for detecting the shading effect of the blocking cover 10. A slot hole 6 is set through the top of the storage plate 5. The slot hole 6 is used to take the storage plate 5 out of the card slot 4 and the reagent box 1. A sponge pad 18 is installed on the top wall of the top cover 3, and the bottom of the sponge pad 18 fits with the top of the blocking cover 10.

[0025] Furthermore, when using the reagent kit 1, first open the top cover 3, then pull the blocking cover 10 upwards so that the limit block 9 is no longer engaged with the limit hole 7, and then observe the color change of the photosensitive material on the surface of the photochromic ball 11 in the blocking cover 10. If there is no change, it means that the light-shielding property of the blocking cover 10 is good, otherwise it means that there is a defect and the light-shielding property is poor. Then touch the hygroscopic cotton 12 to feel its touch and judge the storage environment of the reagent in the blocking cover 10 in the reagent kit 1. The slot 6 is set to take the storage plate 5 out of the reagent kit 1, and the sponge pad 18 is set to cooperate with the limit box 2 to protect the disposable liquid storage tube 16 in the reagent kit 1.

[0026] See also Figure 3 and Figure 6 An embodiment of the present invention is a rapid detection kit for swine fever antibodies, wherein a sponge block 13 is embedded and installed inside the kit 1, the sponge block 13 is located below the storage plate 5, and the top of the sponge block 13 is provided with evenly arranged slots 14, and slots 1 8 and slots 2 14 are both provided with equidistantly arranged plug boards 15, and the tops of multiple groups of plug boards 15 are connected to disposable liquid storage tubes 16, and the disposable liquid storage tubes 16 are located on the inner side of the baffle 10, and the outer surfaces of the multiple groups of disposable liquid storage tubes 16 are provided with connecting plates 17, and the connecting plates 17 between every two disposable liquid storage tubes 16 can be rotatably separated, and the interior of each group of disposable liquid storage tubes 16 is respectively filled with reagents required for swine fever antibody detection.

[0027] Furthermore, by setting up multiple groups of disposable liquid storage tubes 16, each group has multiple single disposable liquid storage tubes 16 connected by a tearable connecting plate 17, each group of different disposable liquid storage tubes 16 contains different amounts of detection reagents. When in use, rotate the single disposable liquid storage tube 16 to separate the connecting plates 17, and then the single disposable liquid storage tube 16 can be removed from the group of disposable liquid storage tubes 16. Then, according to the detection requirements and steps, the reagent in the disposable liquid storage tube 16 can be dropped into the disposable test tube 22 to mix with the sample.

[0028] See also Figure 2 , Figure 7 , Figure 8 and Fig. 9 , an embodiment of the present invention: a rapid detection kit for swine fever antibodies, wherein both sides of the outer walls of the kit 1 are provided with storage grooves, a storage frame 19 is slidably placed inside one group of the storage grooves, and the bottom of the storage frame 19 is in contact with the bottom wall of the kit 1, a partition 20 is provided inside the storage frame 19, and the partition 20 is used to divide the storage frame 19 into two equal spaces, and a storage frame 21 and a partition frame 24 are installed on the bottom wall of the storage frame 19, and the partition frame 24 is located on one side of the storage frame 21, and evenly arranged disposable test tubes 22 are placed inside the storage frame 21, and the top of the disposable test tube 22 is threadedly connected with a sealing cover 23 The disposable test tubes 22 are detachably connected to each other, a disposable pre-coated detection plate is placed inside the partition frame 24, and evenly arranged disposable test strips 25 are placed inside the storage frame 19. The partition 20 is located between the partition frame 24 and the disposable test strips 25. A storage block 26 is slidably placed inside another group of storage slots. The top of the storage block 26 is provided with a placement hole 27 with different apertures. The placement hole 27 is used to place disposable test tubes 22 of different sizes. A drawstring is installed on one side outer wall of the storage frame 19 and the storage block 26. The drawstring is used to pull the storage frame 19 and the storage block 26 out of the storage slot on the reagent kit 1.

[0029] Furthermore, a storage frame 19 and a storage block 26 are also provided inside the reagent box 1. When in use, the drawstrings at the corresponding positions are pulled as needed, and then the storage frame 19 or the storage block 26 can be pulled out from the storage slot. The storage frame 19 is divided into storage space by a partition 20, half of which is used to place a storage frame 21 and a partition frame 24, and the other half is used to place a well-packaged disposable test paper 25. Disposable test tubes 22 are placed inside the storage frame 21, and each disposable test tube 22 is also detachably designed, and the number of disposable test tubes 22 can be selected according to usage needs. The disposable test tubes 22 are equipped with sealing covers 23, and the disposable test tubes 22 can be sealed by the sealing covers 23 during detection, so as to facilitate the sealing and placement reaction of the mixed liquid for detection in the disposable test tubes 22, improve the flexibility during detection, and avoid the waste of disposable test tubes 22. At the same time, a packaged disposable pre-coated detection plate is placed inside the partition frame 24, and the detection antigens of the disposable pre-coated detection plate are recombinant P54 protein and P72 protein, thereby improving the accuracy of detection.

[0030] See also Figure 1 and Figure 5 An embodiment of the present invention is a rapid detection kit for swine fever antibodies, wherein the outer surfaces of the kit 1 and the top cover 3 are sleeved with symmetrically arranged limit boxes 2, and the surfaces of the two groups of limit boxes 2 close to each other are installed with limit plates 28, and the inner surfaces of the two groups of limit plates 28 are respectively fitted with the outer surfaces of the top cover 3 and the kit 1, one side outer wall of one group of limit plates 28 is embedded with a magnet 29, and one side outer wall of the other group of limit plates 28 is embedded with an iron block 30, and one side outer wall of the iron block 30 is fitted with one side outer wall of the magnet 29.

[0031] Furthermore, when the reagent box 1 is not needed, the top cover 3 is covered on the reagent box 1, and then the two sets of limit boxes 2 are put on both sides of the reagent box 1 and the top cover 3, and the limit boxes 2 are pushed toward the middle, and then the limit boxes 2 are firmly fixed on the reagent box 1 and the top cover 3 through the attraction of the magnet 29 and the iron block 30 on the limit plate 28, thereby preventing the items in the reagent box 1 from being spilled and damaged due to accidental contact and falling, thereby improving the protection of the reagent box 1.

[0032] Working principle: first, pull the limit box 2 to both sides so that the magnet 29 is no longer attracted to the iron block 30, and the limit plates 28 on the two groups of limit boxes 2 are separated, and then the limit box 2 can be removed from the reagent box 1 and the top cover 3, and then the storage frame 19 is pulled outward to take out the disposable test tubes 22 and disposable pre-coated test plates and disposable test strips 25 required for the test, and then the baffle 10 is pulled upward so that the limit block 9 is no longer engaged with the limit hole 7, and then the baffle 10 can be removed from the surface of the disposable liquid storage tube 16, and after removing the baffle 10, immediately observe the color change of the light-sensitive color-changing ball 11 inside it and the touch of the moisture-absorbing cotton 12, so as to judge the storage condition of the reagent in the disposable liquid storage tube 16 inside the baffle 10, so as to avoid the situation where the reagent is invalid and affects the accuracy of the test result; Then, take out a group of disposable liquid storage tubes 16 placed in slot 1 8, gently break one of the disposable liquid storage tubes 16, so as to separate the connecting plates 17 on each disposable liquid storage tube 16, so that the disposable liquid storage tubes 16 containing different reagents become independent individuals, and then use the disposable pre-coated detection plate and the disposable detection test paper 25 to perform multiple swine fever antibody tests on the collected samples, and compare the test results using different detection antigens, so as to improve the accuracy of the test results and avoid the test results being affected by specific antibodies and antibodies produced by other ruminant virus infections.

[0033] It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above and that the invention can be implemented in other specific forms without departing from the spirit or essential features of the invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations falling within the meaning and scope of the equivalent elements of the claims be included in the invention. Any reference numeral in a claim should not be considered as limiting the claim to which it relates.

Claims

1. A rapid detection kit for swine fever antibodies, characterized in that: The kit comprises a kit (1), wherein the kit (1) uses recombinant proteins P54 and P72 as detection antigens to detect classical swine fever antibodies; The top of the reagent box (1) is fitted with a top cover (3), and both inner walls of the reagent box (1) are provided with card slots (4). A storage plate (5) is fitted inside the card slots (4), and the top of the storage plate (5) is penetrated with evenly arranged limiting holes (7) and a slot one (8). The limiting holes (7) are located on both sides of the slot one (8). A limiting block (9) is fitted inside the limiting holes (7), and a blocking cover (10) is installed on the top of the limiting block (9). The size of the blocking cover (10) is larger than the size of the slot one (8). Evenly arranged light-sensitive color-changing balls (11) and moisture-absorbing cotton (12) are installed on the inner side wall of the blocking cover (10), and the moisture-absorbing cotton (12) is located below the light-sensitive color-changing balls (11); The outer surface of the light-sensitive color-changing ball (11) is sprayed with a light-sensitive material for detecting the light-shielding effect of the shielding cover (10).

2. A rapid detection kit for classical swine fever antibodies according to claim 1, characterized in that: The reagent kit (1) has a sponge block (13) embedded inside. The sponge block (13) is located below the storage plate (5). The top of the sponge block (13) is provided with slots (14) arranged evenly. Insertion plates (15) arranged evenly are placed inside the slots (8) and the slots (14). The tops of the plurality of groups of insertion plates (15) are connected to disposable liquid storage tubes (16). The disposable liquid storage tubes (16) are located on the inner side of the baffle (10). The outer surfaces of the plurality of groups of disposable liquid storage tubes (16) are provided with connecting plates (17). The connecting plates (17) between every two disposable liquid storage tubes (16) can be rotatably separated. The inside of each group of disposable liquid storage tubes (16) is respectively filled with reagents required for swine fever antibody detection.

3. A rapid detection kit for classical swine fever antibodies according to claim 1, characterized in that: The outer walls of both sides of the reagent box (1) are provided with storage slots, a storage frame (19) is slidably placed inside one group of the storage slots, and the bottom of the storage frame (19) is in contact with the bottom wall of the reagent box (1), a partition (20) is provided inside the storage frame (19), and the partition (20) is used to divide the storage frame (19) into two equal spaces, and a storage frame (21) and a partition frame (24) are installed on the bottom wall of the storage frame (19), and the partition frame (24) is located in the storage frame (21). On one side, evenly arranged disposable test tubes (22) are placed inside the storage frame (21), the top of the disposable test tube (22) is threadedly connected to a sealing cover (23), and the disposable test tubes (22) are detachably connected to each other. A disposable pre-coated test plate is placed inside the partition frame (24), and evenly arranged disposable test strips (25) are placed inside the storage frame (19), and the partition plate (20) is located between the partition frame (24) and the disposable test strips (25).

4. A rapid detection kit for classical swine fever antibodies according to claim 1, characterized in that: The outer surfaces of the reagent box (1) and the top cover (3) are sleeved with symmetrically arranged limit boxes (2), and the surfaces of the two sets of limit boxes (2) close to each other are both installed with limit plates (28), and the inner surfaces of the two sets of limit plates (28) are respectively in contact with the outer surfaces of the top cover (3) and the reagent box (1), one side outer wall of one set of limit plates (28) is embedded with a magnet (29), and one side outer wall of the other set of limit plates (28) is embedded with an iron block (30), and one side outer wall of the iron block (30) is in contact with one side outer wall of the magnet (29).

5. A rapid detection kit for classical swine fever antibodies according to claim 3, characterized in that: Another group of the storage slots has storage blocks (26) slidably placed inside, and the tops of the storage blocks (26) are provided with storage holes (27) with different apertures, and the storage holes (27) are used to place disposable test tubes (22) of different sizes.

6. A rapid detection kit for classical swine fever antibodies according to claim 5, characterized in that: A drawstring is installed on one side outer wall of the storage frame (19) and the storage block (26), and the drawstring is used to pull the storage frame (19) and the storage block (26) out of the storage slot on the reagent box (1).

7. A rapid detection kit for classical swine fever antibodies according to claim 1, characterized in that: A slot (6) is provided through the top of the storage plate (5), and the slot (6) is used to remove the storage plate (5) from the card slot (4) and the reagent box (1).

8. A rapid detection kit for classical swine fever antibodies according to claim 1, characterized in that: A sponge pad (18) is installed on the top wall of the top cover (3), and the bottom of the sponge pad (18) fits the top of the baffle (10).

9. A method for using a rapid detection kit for classical swine fever antibodies, applicable to a rapid detection kit for classical swine fever antibodies according to any one of claims 1 to 4, characterized in that: The method of using the test kit is as follows: S1. First, pull the limit box (2) to both sides so that the magnet (29) is no longer attracted to the iron block (30), and then the limit box (2) can be removed from the reagent box (1) and the top cover (3); S2, then pull the storage frame (19) outward to take out the number of disposable test tubes (22) and disposable pre-coated test plates and disposable test strips (25) required for the test; S3, then pull the blocking cover (10) upwards so that the limiting block (9) is no longer engaged with the limiting hole (7), and then take out a set of disposable liquid storage tubes (16) placed in the slot one (8); S4. Finally, a set of disposable liquid storage tubes (16), disposable pre-coated test plates and disposable test strips (25) are used to perform multiple swine fever antibody tests on the collected samples.

10. The method for using a rapid detection kit for classical swine fever antibodies according to claim 9, characterized in that: In S3, it also includes: after removing the shield (10), immediately observing the color change of the light-sensitive color-changing ball (11) inside the shield (10) and the touch of the moisture-absorbing cotton (12), so as to judge the preservation condition of the reagent in the disposable liquid storage tube (16) inside the shield (10).

Citation Information

Patent Citations

  • An African swine fever virus antibody ELISA detection kit and its preparation method

    CN111929433B

  • Multi-grid swine fever antibody detection kit

    CN209764880U

  • African swine fever antibody detection kit

    CN212244595U

  • Classical swine fever antibody detection kit convenient to distinguish

    CN216233634U