Deodorizing flora liquid, natural acquisition method thereof and application of deodorizing flora liquid in rubber primary processing

Abstract

The invention discloses a deodorization flora liquid, a natural acquisition method thereof and application of the deodorization flora liquid in rubber primary processing, and belongs to the field of natural rubber processing, and the deodorization flora liquid comprises the steps of bacteria-containing soil sample screening, flora purification, flora stability testing and flora amplification culture. Wherein the bacteria-containing soil sample is from a whey waste dumping place, a secondary glue stacking place or soil which is subjected to whey treatment and has an odorless fermentation phenomenon; deodorizing flora purification comprises the following steps: by using whey waste obtained by rubber processing as a culture medium, removing antagonistic bacteria through multi-generation stable culture, detecting flora stability through high-throughput sequencing, and carrying out standing fermentation culture, so as to obtain a deodorizing flora solution with an efficient deodorizing effect. When being applied to deodorization treatment of rubber primary processing, the strain can obviously reduce the emission of malodorous gas in each production and processing link of natural rubber, has the advantages of wide flora source, simplicity in operation, no need of large-scale equipment and expensive reagents, low cost and the like, is expected to be popularized and applied in rubber primary processing, and realizes green production of natural rubber.

Description

Technical Field

[0001] The invention belongs to the field of natural rubber processing, and in particular relates to a method for obtaining deodorizing bacterial liquid from nature and an application of the method in primary processing of rubber. Background Art

[0002] Rubber refers to a highly elastic polymer material with reversible deformation. It is elastic at room temperature and can produce large deformation under very small external force, and can return to its original shape after the external force is removed. Rubber is divided into natural rubber and synthetic rubber. Natural rubber is processed by extracting latex from rubber trees, rubber grass and other plants. In addition to a large amount of rubber hydrocarbons, latex also contains many non-rubber components mainly composed of proteins and sugars. These non-rubber components provide rich C and N sources for the growth of microorganisms. In the processing of natural rubber, the rubber bags directly harvested from the forest contain rich non-rubber nutrients. In the anaerobic or low-oxygen environment formed by the raw rubber package, these non-rubber nutrients produce a large amount of malodorous substances containing sulfur, indole and low-level fatty acids through anaerobic fermentation. These malodorous substances are released during the transportation and storage of the rubber bags, and in various links such as natural rubber harvesting and primary processing, causing malodorous odor pollution. The emission of malodorous gases seriously affects the air quality in the rubber planting and production areas. The high cost of malodorous odor treatment adds additional costs to the natural rubber primary processing industry.

[0003] Therefore, there is an urgent need to find a method that can effectively remove, inhibit and control the malodorous gases in the primary processing of rubber. The current gas treatment methods lack the development and utilization of local deodorization resources based on ecological principles. For example, EM bacteria technology includes formulated microbial communities, which are mainly used for the treatment of domestic sewage and agricultural production waste, and the application scenarios are mainly highly diluted water bodies; plant deodorant liquid technology is mainly used in factory discharge spray systems, because it is a process for extracting natural plant deodorizing substances, the cost is relatively high, which is unaffordable for the natural rubber primary processing industry; chemical deodorization will introduce inorganic chemical pollution, which is not feasible for the rubber primary processing industry that requires a large amount of water to wash the rubber. Summary of the invention

[0004] Aiming at the problem that the existing methods cannot efficiently and cost-effectively remove the foul odors generated by anaerobic fermentation of non-rubber organic matter in the natural rubber processing process, the present invention provides a deodorizing bacterial liquid with good deodorizing ability, which is used for treating the odors generated by the stacking and processing of solid raw rubber bags.

[0005] In order to solve the above technical problems, the present invention provides a method for obtaining a deodorizing bacteria group in nature, comprising the following steps:

[0006] (1) Screening of soil samples containing bacteria: 5 to 10 spots with a length of 1 to 3 m were marked at the source of the bacteria. 2Extraction areas, remove surface debris, pour 1 to 50L of rubber stinking whey into each extraction area, cover the surface with a waterproof film and keep it breathable, and after fermentation for 1 to 15 days, dig 0.1 to 5kg of soil samples at a depth of 10 to 15cm, perform nasal test and extract with activated carbon adsorption method, cold trap adsorption method or solid phase microextraction method, and detect the content of target odor components by GC-MS. The nasal test odorless soil samples with the content of target odor components <10ng / kg are taken as bacteria-containing soil samples;

[0007] (2) Screening and culturing deodorizing bacteria: 0.1-5 kg ​​of the bacteria-containing soil sample screened in step (1) is placed in 100 L of natural rubber whey diluted 10-100 times for fermentation treatment, and 3-50 bacteria-containing soil samples are repeatedly screened to screen out the precipitated sludge that can cause the natural rubber whey to stratify and precipitate, and the supernatant has no odor when detected by nose. After circulating the culture for 2-5 times, it is cultured with fresh anaerobic fermentation whey, and the culture is repeated for 3-50 samples. The sludge suspension that can quickly and odorlessly decompose the rubber whey within 0-15 days is screened out as the bacteria-containing suspension and inoculated into the treated rubber whey, and the fermentation culture is continued for multiple generations to obtain a composite bacterial liquid with a stable deodorizing effect;

[0008] (3) Microbial community stability detection: Take the composite microbial community liquid of step (2), add liquid nitrogen to freeze, extract total DNA using a soil microbial DNA extraction kit, amplify with environmental microbial bacteria 515F / 926R and fungal universal ITS sequence primers, and perform high-throughput sequencing to detect the stability of the microbial community;

[0009] (4) Fermentation culture: The composite bacterial community liquid is subjected to static fermentation culture, and the supernatant rich in active deodorizing metabolites is used as the deodorizing bacterial community liquid, and the turbid sedimentation liquid of static fermentation is used as the bacteria-containing sedimentation liquid.

[0010] Preferably, the source of the bacterial community includes the odorless soil matrix of the whey waste dumping site of secondary rubber production, the odorless soil matrix of the secondary rubber stacking site and / or the sludge produced by the odorless fermentation phenomenon after being treated with rubber smelly whey.

[0011] Preferably, the target odor components include methyl sulfides, indoles, methyl phenols and / or volatile fatty acids, wherein the methyl sulfides refer to dimethyl disulfide and trimethyl trisulfide; the indoles refer to indole and methyl indole; the methyl phenols refer to p-methylphenol; and the volatile fatty acids refer to butyric acid and isovaleric acid.

[0012] Preferably, the adsorption temperature of the cold trap adsorption method is -70°C, the adsorption time is ≥3h, and after adsorption, the cold trap is eluted with redistilled ethyl acetate and dried.

[0013] Preferably, the treated rubber whey in step (2) is freshly separated rubber whey that has not been anaerobic fermented or pasteurized rubber whey that has been anaerobic fermented.

[0014] Preferably, the acquisition method further comprises step (5) of testing the deodorization effect: treating the malodorous secondary glue block by a surface spray method or an immersion method to test the deodorization effect of the deodorizing bacterial community liquid, wherein the deodorizing bacterial community liquid that can remove the malodor within 15 seconds to 30 minutes according to the test result is a high-efficiency deodorizing bacterial community liquid.

[0015] In order to solve the above technical problems, the present invention provides a deodorizing bacterial liquid obtained by the above method.

[0016] In order to solve the above technical problems, the present invention provides the use of the above deodorizing bacteria liquid in the initial processing of rubber.

[0017] Preferably, the application is specifically as follows: spraying the deodorizing bacteria liquid at a ratio of 1 to 100 L / ton in the pile area from high altitude or spraying the deodorizing bacteria liquid on the wet rubber in the feeding area of ​​the rubber drying bed at a ratio of 1 to 50 L / ton; spraying the high-efficiency deodorizing bacteria liquid on the surface of the malodorous raw material pile in the secondary rubber production area of ​​the planting area at a ratio of 1 to 20 L / ton,

[0018] Preferably, the amount of deodorizing bacteria liquid in the stockpile environment is 0.1 to 5 L / m 2 , to add or strengthen the deodorizing microorganisms in the environment.

[0019] Preferably, the bacterial sedimentation liquid in step (4) can also be added to the floor of the stockpile area or the rubber washing pool for deodorization, wherein: the amount of bacterial sedimentation liquid required for the floor of the stockpile area is 1.0 L / m 2 ; The proportion of bacterial sedimentation liquid added to the glue washing pool is 1% to 10%.

[0020] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0021] The present invention starts from the ecological principle of microbial enrichment, extracts, separates and cultivates natural deodorizing microbial flora from the rubber production area environment that discharges high-concentration rubber whey but does not discharge malodorous gas, and cultivates for multiple times to remove antagonistic bacteria. After sequencing to detect the stability of the flora, the flora is expanded by static culture. The supernatant of the static culture is used as the deodorizing flora liquid for deodorizing the surface of malodorous raw material piles in the stockpiling area, the feeding area of ​​the rubber drying bed, and the secondary rubber production area of ​​the planting area. The turbid sedimentation liquid also contains a lot of deodorizing microorganisms, which can also be used for deodorizing the surface of the rubber pile or the rubber washing pool in the primary processing of rubber. The problem of malodor generated by anaerobic fermentation of non-rubber organic matter in the natural rubber processing process can be removed efficiently and at low cost. The flora has a wide source, is simple to operate, does not require large equipment and expensive reagents, is low in cost, and is expected to be promoted and applied in the primary processing of rubber to realize the green production of natural rubber. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a flow chart of the method for obtaining the odor bacteria liquid from nature of the present invention;

[0023] Figure 2 This is the GC-MS spectrum of the malodorous odor components in natural rubber;

[0024] Figure 3 It is the GC-MS spectrum of the malodorous odor components in the soil sample containing bacteria;

[0025] Figure 4 It is the spectrum of deodorization effect test results. DETAILED DESCRIPTION

[0026] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] Example 1

[0028] This embodiment provides a method for obtaining a deodorizing bacterial liquid from nature, and the process is as follows: Figure 1 As shown, the following steps are included:

[0029] (1) Screening of soil samples containing bacteria: The surface of the land where waste whey from secondary rubber production was dumped for a long time was selected as the source of bacterial flora. A 1m2 area was marked out at the source of bacterial flora. 2 Extraction area, clear 2m around 2 Remove the surface debris within the range and remove the surface soil 3cm deep, pour 20L of smelly whey, and cover the surface with 2m 2 A wide waterproof membrane is used to support a shed-like structure with a high center and a low outside. After 7 days of fermentation, the waterproof membrane is uncovered, and 5 cm of topsoil is removed. Soil samples within a depth of 5 to 15 cm are taken. The soil is first tested for malodor by nose test, and then adsorbed for 3 hours using a -70 ° C cold trap adsorption method. The cold trap is eluted with redistilled ethyl acetate, and the malodorous odor components are detected by GC-MS after drying and concentration. Figure 2 The results are shown as follows: 15 volunteers who took nasal tests all reported no odor, and the target odor components included dimethyl disulfide, trimethyl trisulfide, indole, methyl indole, p-methylphenol, butyric acid and isovaleric acid. The content of each component was lower than the instrument detection limit of 1ng / kg. The soil sample can be used as a bacterial soil sample for subsequent extraction of deodorizing bacteria.

[0030] (2) Screening and culturing deodorizing bacteria: Take 1 kg of the bacteria-containing soil sample screened in step (1), place it in 10 L of rubber whey and add 90 L of clean water to dilute 10 times of natural rubber whey for fermentation treatment, repeat the screening of 30 bacteria-containing soil samples, screen out the precipitated sludge that can cause natural rubber whey to stratify and precipitate, and the supernatant has no odor when tested by nose, and after 3 cycles of cultivation, use fresh anaerobic fermentation whey for cultivation, repeat the cultivation of 30 samples, screen out the sludge suspension that can quickly and odorlessly decompose rubber whey within 3 days, and inoculate it as the bacteria-containing suspension on the treated rubber whey, continue the fermentation and cultivation for multiple generations, and obtain a composite bacterial community liquid with a stable deodorizing effect, at which time the antagonistic bacteria have been mostly removed;

[0031] (3) Microbial community stability detection: 20 mL of the composite microbial community liquid of step (2) was taken, and after freezing in liquid nitrogen, total DNA was extracted using a soil microbial DNA extraction kit. After amplification using environmental microbial bacteria 515F / 926R and fungal universal ITS sequence primers, high-throughput sequencing was performed to detect the stability of the microbial community. After testing, the bacterial species in the microbial sludge suspension mainly included wild strains such as firmicutes, actinomycetes, photosynthetic bacteria, and oxidative bacteria;

[0032] (4) Fermentation culture: The composite bacterial community liquid is subjected to static fermentation culture, and the supernatant rich in active deodorizing metabolites is used as the deodorizing bacterial community liquid for deodorizing the surface of rubber products. The static fermentation sedimentation liquid also contains deodorizing microbial flora, which can be used as the bacterial sedimentation liquid for microbial addition and rejuvenation treatment in the processing environment;

[0033] (5) Deodorization effect test: The deodorization bacterial community liquid was diluted 10 times with freshly isolated non-anaerobic fermented whey and fermented for 7 days. The supernatant was tested by soaking the malodorous gum blocks. After 30 seconds of treatment, the malodorous gum blocks became odorless. GC-MS detection showed no malodorous odor components, such as Figure 4 As shown, it is a highly effective deodorizing bacterial liquid.

[0034] from Figure 2 and Figure 4 It can be seen that the deodorizing bacterial liquid obtained in this example can effectively remove the malodorous odor substances produced in the production of natural rubber.

[0035] Application of deodorizing bacteria liquid in rubber primary processing:

[0036] Application 1: Spray the deodorizing bacteria liquid at a high altitude in the stockpiling area of ​​a rubber processing plant at a dosage of 10L / ton, and the malodor will disappear and no malodor will be generated.

[0037] Application 2: Adding bacterial sedimentation liquid at a ratio of 5% into the rubber washing pool of the rubber processing plant will eliminate the malodor and no malodor will be generated.

[0038] Example 2

[0039] This embodiment provides a method for obtaining a deodorizing bacteria liquid from nature, comprising the following steps:

[0040] (1) Screening of bacterial soil samples: The odorless soil matrix at the secondary glue stack was selected as the bacterial source. A 3m 2 Extraction area, clear 2m around 2 Remove the surface debris within the range and remove the surface soil 3cm deep, dump 50L of smelly whey, and cover the surface with 6m 2 A wide waterproof membrane is used to support a shed-like structure with a high center and a low outside. After 3 days of fermentation, the waterproof membrane is uncovered, 10 cm of topsoil is removed, and soil samples within a depth of 10 to 15 cm are taken. The soil is first tested for odor by nose test, and then the odor substances are extracted by activated carbon adsorption method and the content of odor components is tested by GC-MS. The results are as follows: 15 volunteers who took nose tests all reported no odor. The target odor components include dimethyl disulfide, trimethyl trisulfide, indole, methyl indole, p-methylphenol, butyric acid and isovaleric acid. The content of each component is less than 2 ng / kg. The soil sample can be used as a bacterial soil sample for subsequent extraction of deodorizing bacteria.

[0041] (2) Purification and cultivation of deodorizing bacteria: 5 kg of the bacteria-containing soil sample screened in step (1) was placed in 1 L of rubber whey and 99 L of clean water to dilute 100 times of natural rubber whey for fermentation treatment. 50 bacteria-containing soil samples were screened repeatedly to screen out the precipitated sludge that can cause natural rubber whey to stratify and precipitate, and the supernatant has no odor when tested by nose. After 5 cycles of cultivation, fresh anaerobic fermentation whey was used for cultivation. The culture was repeated for 20 samples. The sludge suspension that can quickly and odorlessly decompose rubber whey within 5 days was screened out and inoculated as the bacteria-containing suspension onto the treated rubber whey. The fermentation and cultivation were continued for multiple generations to obtain a composite bacterial community liquid with a stable deodorizing effect. At this time, most of the antagonistic bacteria have been removed.

[0042] (3) Microbial community stability detection: 20 mL of the composite microbial community liquid of step (2) was taken, and after freezing in liquid nitrogen, total DNA was extracted using a soil microbial DNA extraction kit. After amplification using environmental microbial bacteria 515F / 926R and fungal universal ITS sequence primers, high-throughput sequencing was performed to detect the stability of the microbial community. After testing, the bacterial species in the microbial sludge suspension mainly included wild strains such as firmicutes, actinomycetes, photosynthetic bacteria, and oxidative bacteria;

[0043] (4) Expanded culture: The composite bacterial community liquid is cultured by static fermentation, and the supernatant rich in active deodorizing metabolites is used as the deodorizing bacterial community liquid for deodorizing the surface of rubber products. The static fermentation sedimentation liquid also contains deodorizing microbial flora, which can be used as the bacterial sedimentation liquid for microbial addition and rejuvenation treatment in the processing environment;

[0044] (5) Deodorization effect test: The deodorizing bacterial community liquid was diluted 10 times with freshly isolated non-anaerobic fermented whey and fermented for 7 days. The supernatant was tested by soaking the malodorous gum blocks. After 30 seconds of treatment, the malodorous gum blocks became odorless. GC-MS detection showed no malodorous odor components, indicating that it was a highly efficient deodorizing bacterial community liquid.

[0045] Add deodorizing bacteria liquid at a dosage of 10L / ton in the baking rubber feeding area and wet rubber block area of ​​the processing plant, and the bad smell disappears and no bad smell is generated;

[0046] At 1.0L / m 2 By spraying the bacterial sedimentation liquid on the ground of the raw material planting villages and secondary rubber stockpiling areas, the bad smell disappears and no bad smell is generated.

[0047] Example 3

[0048] This embodiment provides a method for obtaining a deodorizing bacteria liquid from nature, comprising the following steps:

[0049] (1) Screening of bacterial soil samples: The natural soil around the rubber plantation was treated with whey to produce odorless fermented sludge as the bacterial source. A 2m2 area was marked out at the bacterial source. 2 Extraction area, clear 2m around 2 Remove the surface debris within the range and remove the surface soil 3cm deep, dump 40L of smelly whey, and cover the surface with 4m 2 A wide waterproof membrane is used to support a shed-like structure with a high center and a low outside. After 15 days of fermentation, the waterproof membrane is uncovered, 10 cm of topsoil is removed, and soil samples within a depth of 10 to 15 cm are taken. The soil is first tested for odor by nose test, and then the odor substances are extracted by solid phase microextraction and the content of odor components is tested by GC-MS. The results are as follows: 15 volunteers who took nose tests all reported no odor. The target odor components include dimethyl disulfide, trimethyl trisulfide, indole, methyl indole, p-methylphenol, butyric acid and isovaleric acid. The content of each component is less than 1.5 ng / kg. The soil sample can be used as a bacterial soil sample for subsequent extraction of deodorizing bacteria.

[0050] (2) Screening and culturing deodorizing bacteria: Take 2 kg of the bacteria-containing soil sample screened in step (1), place it in 5 L of rubber whey and add 95 L of clean water to dilute 20 times of natural rubber whey for fermentation treatment, repeat the screening of 10 bacteria-containing soil samples, screen out the precipitated sludge that can cause natural rubber whey to stratify and precipitate, and the supernatant has no odor when tested by nose, and after circulating the culture for 2 more times, use fresh anaerobic fermentation whey for culture, repeat the culture for 10 samples, screen out the sludge suspension that can quickly and odorlessly decompose rubber whey within 15 days, and inoculate it as the bacteria-containing suspension on the treated rubber whey, continue the fermentation and culture for multiple generations, and obtain a composite bacterial community liquid with a stable deodorizing effect, at which time the antagonistic bacteria have been mostly removed;

[0051] (3) Microbial community stability detection: 20 mL of the composite microbial community liquid of step (2) was taken, and after freezing in liquid nitrogen, total DNA was extracted using a soil microbial DNA extraction kit. After amplification using environmental microbial bacteria 515F / 926R and fungal universal ITS sequence primers, high-throughput sequencing was performed to detect the stability of the microbial community. After testing, the bacterial species in the microbial sludge suspension mainly included wild strains such as firmicutes, actinomycetes, photosynthetic bacteria, and oxidative bacteria;

[0052] (4) Fermentation culture: The composite bacterial community liquid is subjected to static fermentation culture, and the supernatant rich in active deodorizing metabolites is used as the deodorizing bacterial community liquid for deodorizing the surface of rubber products. The static fermentation sedimentation liquid also contains deodorizing microbial flora, which can be used as the bacterial sedimentation liquid for microbial addition and rejuvenation treatment in the processing environment;

[0053] (5) Deodorization effect test: The deodorizing bacterial community liquid was diluted 10 times with freshly isolated non-anaerobic fermented whey and fermented for 7 days. The supernatant was tested by soaking the malodorous gum blocks. After 30 seconds of treatment, the malodorous gum blocks became odorless. GC-MS detection showed no malodorous odor components, indicating that it was a highly efficient deodorizing bacterial community liquid.

[0054] Spray high-efficiency deodorizing bacteria liquid at a dosage of 10L / ton in the stockpile area of ​​the secondary rubber production in the planting area, and the stench disappears, and no stench or odor is generated;

[0055] Adding bacterial sedimentation liquid at a ratio of 5% into the rubber washing pool of the rubber processing plant will eliminate the bad smell and no more bad odor will be generated.

[0056] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them. A person skilled in the art should understand that the technical solutions described in the above embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements will not cause the essence of the corresponding technical solutions to deviate from the protection scope of the present invention.

Claims

1. A method for obtaining a deodorizing bacterial liquid from nature, characterized in that: The following steps are involved: (1) Screening of soil samples containing bacteria: 5 to 10 spots with a length of 1 to 3 m were marked at the source of the bacteria. 2 Extraction areas, remove surface debris, pour 1 to 50L of rubber stinking whey into each extraction area, cover the surface with a waterproof film and keep it breathable, and after fermentation for 1 to 15 days, dig 0.1 to 5kg of soil samples at a depth of 10 to 15cm, perform nasal test and extract with activated carbon adsorption method, cold trap adsorption method or solid phase microextraction method, and detect the content of target odor components by GC-MS. The nasal test odorless soil samples with the content of target odor components <10ng / kg are taken as bacteria-containing soil samples; (2) Screening and culturing deodorizing bacteria: 0.1-5 kg ​​of the bacteria-containing soil sample screened in step (1) is placed in 100 L of natural rubber whey diluted 10-100 times for fermentation treatment. The operation is repeated for 3-50 bacteria-containing soil samples to screen out the precipitated sludge that can cause the natural rubber whey to stratify and precipitate, and the supernatant has no odor when detected by nose. After circulating the culture for 2-5 times, the sludge is cultured with fresh anaerobic fermentation whey. The culture is repeated for 3-50 samples to screen out the sludge suspension that can quickly and odorlessly decompose the rubber whey within 0-15 days as the bacteria-containing suspension and inoculate it into the treated rubber whey. The culture is continued stably for multiple generations to obtain a composite bacterial liquid with a stable deodorizing effect. (3) Microbial community stability detection: Take the composite microbial community liquid of step (2), add liquid nitrogen to freeze, extract total DNA using a soil microbial DNA extraction kit, amplify with environmental microbial bacteria 515F / 926R and fungal universal ITS sequence primers, and perform high-throughput sequencing to detect the stability of the microbial community; (4) Fermentation culture: The composite bacterial liquid is subjected to static fermentation culture, and the supernatant rich in active deodorizing metabolites is taken as the deodorizing bacterial liquid.

2. The method for obtaining the deodorizing bacteria liquid from nature according to claim 1, characterized in that: The source of the bacterial community in step (1) includes the odorless soil matrix of the whey waste dumping site of secondary rubber production, the odorless soil matrix of the secondary rubber stacking site and / or the odorless fermentation sludge produced by the treatment of rubber odorous whey.

3. The method for obtaining a deodorizing bacterial liquid from nature according to claim 1, characterized in that: The target odor components in step (1) include methyl sulfides, indoles, methyl phenols and / or volatile fatty acids.

4. The method for obtaining a deodorizing bacterial liquid from nature according to claim 1, characterized in that: The adsorption temperature of the cold trap adsorption method described in step (1) is -70°C, the adsorption time is ≥3h, and after adsorption, the cold trap is eluted with redistilled ethyl acetate and dried.

5. The method for obtaining the deodorizing bacteria liquid from nature according to claim 1, characterized in that: The treated rubber whey in step (2) is freshly separated rubber whey that has not been anaerobic fermented or pasteurized rubber whey that has been anaerobic fermented.

6. The method for obtaining deodorant bacterial liquid from nature according to claim 1, characterized in that: The method also includes step (5) of testing the deodorization effect: treating the malodorous secondary gel blocks by surface spraying or immersion to test the deodorization effect of the composite bacterial liquid.

7. A deodorizing bacterial liquid obtained by the method according to any one of claims 1 to 6.

8. The deodorizing bacterial liquid according to claim 7, characterized in that: The deodorizing bacteria liquid mainly includes firmicutes, actinomycetes, photosynthetic bacteria and oxidative bacteria.

9. Use of the deodorizing bacterial liquid according to claim 7 or 8 in the primary processing of rubber.

10. Use of the deodorizing bacterial liquid according to claim 9 in rubber primary processing, characterized in that: The applications include high-altitude spraying in the rubber raw material accumulation area, spraying on the wet rubber in the feeding area of ​​the rubber drying bed, spraying on the surface of the malodorous raw material pile in the secondary rubber production area of ​​the planting area, and adding to the surface of the pile area or the rubber washing pool.

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