Accurate matrix collection device of vertical flow constructed wetland model

Abstract

The invention belongs to and discloses an accurate matrix acquisition device for a vertical flow constructed wetland model, which comprises a simulation mechanism, and the simulation mechanism is provided with an auxiliary mechanism in a matched manner; the simulation mechanism comprises a simulation container filled with a matrix, a plurality of matrix sampling ports are longitudinally formed in the side wall of the simulation container, and sampling is carried out in the simulation experiment process; the auxiliary mechanism comprises a semi-cylindrical groove matched with the simulation container, the simulation container is connected in the semi-cylindrical groove in a sliding mode, an ejection assembly is arranged at the bottom end of the semi-cylindrical groove, the ejection assembly is in transmission connection with the simulation container, and the ejection assembly abuts against the end of a matrix in the simulation container; the push-out device is used for pushing out the matrix in the simulation container after the simulation experiment is finished. The device is accurate in matrix collection, high in efficiency, easy and convenient to operate and low in cost, target matrixes of different layers can be accurately collected, the problem that the original matrix structure is effectively protected when the matrixes are collected in different periods is solved, and normal operation of subsequent experiments and reliability of results are ensured.

Description

Technical Field

[0001] The invention belongs to the field of water environment science and technology, and in particular relates to a substrate precision collection device for a vertical flow artificial wetland model. Background Art

[0002] Vertical flow artificial wetland is a new type of sewage treatment process, which is mainly composed of a matrix, aquatic plants growing on the surface, and an ecosystem composed of microbial communities attached to the surface of the matrix. However, due to the large size of artificial wetlands in natural environments, laboratory models are usually used instead of natural artificial wetlands for microscopic experiments. During the simulation, various matrices are usually filled inside the model as habitats for microorganisms and growth matrices for plant roots. Aquatic plants are planted on the surface of the matrix to simulate the wetland system in the natural environment. Sewage flows into the artificial wetland model from the upper or lower end of the model. After entering the model, the matrix will be completely immersed in the sewage. The adsorption effect of the matrix and the degradation effect of the microorganisms attached to the matrix play an important role in removing pollutants in the sewage, thereby achieving the effect of purifying the sewage.

[0003] The richness and diversity of microorganisms attached to the substrate are the main research objects for improving the sewage treatment effect. However, the substrates at different positions and the experimental operation time period will have a huge impact on the microorganisms attached to the substrate, which will greatly reduce the accuracy and reliability of the experimental results. Therefore, how to accurately collect different layers of substrates and reduce the error in the process of collecting substrates is crucial for subsequent experimental scientific analysis. It is difficult for the existing artificial wetland model to accurately collect the target substrate when collecting the substrate, and the collected substrate will inevitably be mixed with other layers of substrates, affecting the reliability of the experimental results. In addition, when the middle and lower layers of the substrate need to be collected in the middle of the experiment, the structure of the upper layer of the substrate will inevitably be destroyed first, resulting in destructive sampling, which will cause the subsequent experiments to fail to run normally and the reliability of the experimental data is worrying. Finally, at the end of the experiment, it is usually necessary to collect the upper, middle and lower layers of the substrate in the artificial wetland model for analysis. However, due to the limited diameter of the cylindrical artificial wetland model, the substrate can usually only be manually grasped by hand. During the grasping process, it is inevitable that the upper layer of the substrate will slip into the lower layer of the substrate, resulting in the collected substrate not being the in-situ substrate, affecting the reliability of the experimental results.

[0004] Therefore, in order to solve the above problems, the present invention provides a substrate precision collection device for a vertical flow artificial wetland model. Summary of the invention

[0005] In order to solve the above technical problems, the present invention proposes a substrate precision collection device for a vertical flow artificial wetland model.

[0006] To achieve the above-mentioned object, the present invention provides a substrate precision collection device for a vertical flow artificial wetland model, comprising a simulation mechanism for simulating a vertical flow artificial wetland, wherein the simulation mechanism is equipped with an auxiliary mechanism for performing precise sampling after the experiment is completed;

[0007] The simulation mechanism comprises a simulation container, the simulation container is filled with a matrix, and a plurality of matrix sampling ports are longitudinally arranged on the side wall of the simulation container to facilitate the collection of matrix samples during the simulation experiment;

[0008] The auxiliary mechanism includes a semi-cylindrical groove adapted to the simulation container, the main body of the simulation container lies horizontally in the semi-cylindrical groove, and an ejection component is provided at the bottom end of the semi-cylindrical groove. The ejection component abuts against the end of the matrix in the simulation container and is used to push the matrix in the simulation container out after the simulation experiment is completed.

[0009] Preferably, the ejection assembly includes a sealing plate fixedly connected to the end of the semi-cylindrical groove, a fixed push rod facing the inner cavity of the semi-cylindrical groove is fixedly connected to the sealing plate, a push plate is fixedly connected to the end of the fixed push rod away from the sealing plate, and the push plate abuts against the bottom end of the matrix in the simulation container to push the matrix out of the simulation container.

[0010] Preferably, the ejection assembly includes a rotating shaft rotatably connected to the bottom end of the semi-cylindrical groove, both ends of the rotating shaft respectively extend out of the semi-cylindrical groove and are provided with rope collectors, a connecting rope is provided on the rope collector, and a hook for fixing the simulation container is provided on the connecting rope.

[0011] Preferably, two symmetrically arranged handles are provided at the top of the side wall of the simulation container, and the hook is hooked on the handles, and the crank is rotated to drive the rope collector to rotate and push out the matrix in the simulation container.

[0012] Preferably, the simulation container comprises a main body for filling the matrix, and the matrix sampling port is arranged on the side wall of the main body; the bottom end of the main body is connected to a base with a bottom sealing via a thread (6).

[0013] Preferably, a sewage inlet is provided on the base, and a water outlet for discharging purified sewage is provided on the top side wall of the main body.

[0014] Preferably, the top of the main body is open and planted with aquatic plants.

[0015] Preferably, the matrix sampling port is located on the side wall of the main body and is provided with a detachable cover, and the sample can be taken by removing the cover.

[0016] Preferably, any one end of the rotating shaft is arranged on a crank which is transmission-connected to the rotating shaft to facilitate the rotation of the rotating shaft.

[0017] Preferably, the matrix in the main body is inoculated with activated sludge from a sewage treatment plant.

[0018] Compared with the prior art, the present invention has the following advantages and technical effects: the present invention discloses a substrate precision collection device for a vertical flow artificial wetland model, which performs simulation experiments through a simulation mechanism and is flexible and convenient to use. The simulation mechanism is equipped with an auxiliary mechanism, which can completely push the substrate out of the simulation container of the simulation mechanism at the end of the experiment, so as to facilitate the collection of substrate samples of different layers and the analysis of different layers of substrates. The collection accuracy is high and can be used for dynamic analysis of microbial communities after the experiment to obtain more accurate data. At the same time, the design of the substrate sampling port can perform non-destructive sampling in the middle of the experiment, and can also collect in-situ sewage from the upper, middle and lower layers of the substrate, which can be used for subsequent analysis of the sewage removal efficiency of different layers of substrates.

[0019] The device of the present invention collects matrix accurately, efficiently, easily and at low cost, can accurately collect target matrix at different layers, and solves the problem of effectively protecting the original matrix structure when collecting matrix at different periods, thereby ensuring the normal operation of subsequent experiments and the reliability of results. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] The drawings constituting a part of the present application are used to provide a further understanding of the present application. The illustrative embodiments and descriptions of the present application are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:

[0021] Figure 1 It is a general schematic diagram of the substrate precision collection device of the vertical flow artificial wetland model of the present invention;

[0022] Figure 2 It is a schematic diagram of the structure of the substrate precision collection device of the vertical flow artificial wetland model of the present invention;

[0023] Figure 3 This is a schematic diagram of the matrix collection auxiliary tool of the present invention;

[0024] Figure 4 It is a working schematic diagram of the auxiliary tool of the present invention;

[0025] Figure 5 A comparison diagram of the substrate precision collection device of the vertical flow artificial wetland model of the present invention;

[0026] In the figure: 1. aquatic plant; 2. water outlet; 3. handle; 4. substrate sampling port; 5. main body; 6. connecting thread; 7. base; 8. water inlet; 9. semi-cylindrical groove; 10. fixed push rod; 11. rope collector; 12. cover; 13. sealing plate; 14. crank; 15. rotating shaft; 16. positioning plate; 17. support; 18. connecting rope; 19. hook; 20. push plate. DETAILED DESCRIPTION

[0027] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0029] Reference Figure 1-Figure 5 As shown, this embodiment provides a substrate precision collection device for a vertical flow artificial wetland model, including a simulation mechanism for simulating a vertical flow artificial wetland, and the simulation mechanism is equipped with an auxiliary mechanism for performing precise sampling at the end of the experiment;

[0030] The simulation mechanism includes a simulation container filled with a matrix, and a plurality of matrix sampling ports 4 are longitudinally arranged on the side wall of the simulation container for sampling during the simulation experiment;

[0031] The auxiliary mechanism includes a semi-cylindrical groove 9 adapted to the simulation container. The main part of the simulation container lies horizontally in the semi-cylindrical groove 9. An ejection component is provided at the bottom end of the semi-cylindrical groove 9. The ejection component abuts against the end of the matrix in the simulation container and is used to push the matrix in the simulation container out after the simulation experiment is completed.

[0032] The present invention discloses a substrate precise collection device for a vertical flow constructed wetland model. Through a simulation mechanism for carrying out simulation experiments, it is flexible and convenient to use. The simulation mechanism is equipped with an auxiliary mechanism, which can push the substrate out of the simulation container of the simulation mechanism completely at the end of the experiment, facilitating the collection of substrate samples at different levels, with high collection accuracy, and can be used to analyze the dynamic changes of microbial communities on substrates at different layers, and more accurate data can be obtained. At the same time, the design of the substrate sampling port 4 enables non-destructive sampling during the mid-term of the experiment, and can also collect in-situ sewage from the upper, middle and lower layers, which can be used for subsequent analysis of the sewage removal efficiency of substrates at different layers. The device of the present invention collects the substrate accurately, efficiently, is easy to operate, and has a low cost. It can precisely collect the target substrates at different layers, and solves the problem of effectively protecting the original substrate structure when collecting substrates at different times, ensuring the normal operation of subsequent experiments and the reliability of the results.

[0033] In a further optimized solution, the ejection assembly includes a sealing plate 13 fixedly connected to the end of the semi-cylindrical groove 9. A fixed push rod 10 facing the inner cavity of the semi-cylindrical groove 9 is fixedly connected to the sealing plate 13. One end of the fixed push rod 10 away from the sealing plate 13 is fixedly connected to a push plate 20. The push plate 20 abuts against the bottom end of the substrate in the main body 5 to push the substrate out of the simulation container. The ejection assembly includes a rotating shaft 15 rotatably connected to the bottom end of the semi-cylindrical groove 9. Both ends of the rotating shaft 15 extend out of the semi-cylindrical groove 9 respectively and are provided with rope winders 11. A connecting rope 18 is wound around the rope winder 11. A hook 19 for fixing the simulation container is arranged on the connecting rope 18. The sealing plate 13 is arranged at one end of the semi-cylindrical groove 9 as the fixed position of the fixed push rod 10. When working, the push plate 20 at the end of the fixed rod extends into the simulation container and abuts against the bottom end of the substrate. The hook 19 is connected to the handle 3, and then the crank 14 is rotated to drive the rotating shaft 15 to rotate. The connecting rope 18 extended out is recovered by the two rope winders 11 driven by the rotating shaft 15. The simulation container is pulled towards the sealing plate 13 by the hook 19 hooked at the end of the connecting rope 18, and the push plate 20 pushes the substrate out of the open end at the top of the simulation container.

[0034] Furthermore, two corresponding positioning plates 16 are arranged on the sealing plate 13. The rotating shaft 15 is rotatably connected between the two positioning plates 16 to play a role in fixing the rotating shaft 15.

[0035] Furthermore, a U-shaped support 17 is fixedly connected to the sealing plate 13. The fixed rod is fixedly connected to the top end of the support 17. The rotating shaft 15 passes through the span at the bottom end of the support 17 without any influence.

[0036] In a further optimized solution, two symmetrically arranged handles 3 are arranged at the top end of the side wall of the simulation container. When pushing out the substrate in the simulation container, the hook 19 is hooked on the handle 3. The handle 3 can facilitate the user to grasp the simulation container, and at the same time, it can also be used as a fixed point for the hook 19 when pushing out the substrate at the end of the experiment, facilitating the application of force.

[0037] In a further optimized solution, the simulation container includes a main body 5 for filling the matrix, and the matrix sampling port 4 is arranged on the side wall of the main body 5; the bottom end of the main body 5 is threadedly connected with a bottom-sealed base 7. The main body 5 is a cylindrical structure, and the inside thereof is filled with the matrix. The bottom end of the base 7 is sealed and installed at the bottom end of the main body 5. After use, the main body 5 and the base 7 can be separated, and the simulation container can be conveniently fixed at a designated position in the laboratory through the base 7.

[0038] Furthermore, the bottom end of the main body 5 and the top end of the base 7 are respectively provided with matching connecting threads 6, and the main body 5 and the base 7 are connected together through the connecting threads 6, which is convenient for loading and unloading.

[0039] Furthermore, the handle 3 is mounted on the top side wall of the main body 5 .

[0040] Furthermore, in this embodiment, the height of the main body 5 is preferably 80 cm, the height of the base 7 is preferably 20 cm, and the outer diameters of the main body 5 and the base 7 are preferably 20 cm.

[0041] Furthermore, the inner diameter of the semicircular groove of this embodiment is preferably 21 cm, so that the main body 5 can move in the semicircular groove.

[0042] Furthermore, the length of the semicircular groove of this embodiment is preferably 200 cm, and the length of the fixing rod is preferably 100 cm, so that the push plate 20 can completely and completely push the matrix in the main body 5 out of the main body 5.

[0043] In a further optimized solution, the base 7 is provided with a water inlet 8 for pumping sewage into the base 7, and the top side wall of the main body 5 is provided with a water outlet 2 for discharging the purified sewage. The water inlet 8 is provided on the base 7, and the water outlet 2 is provided on the top side wall of the main body 5, so that the sewage enters from the bottom, and the purified water overflows from the top, and the sewage is processed in the process of rising.

[0044] In a further optimized solution, the top of the main body 5 is open and planted with aquatic plants 1. The aquatic plants 1 are planted on the top of the substrate, and the aquatic plants 1 are immersed in the treated water. By observing the growth state of the aquatic plants 1, the effect of sewage treatment can be briefly judged.

[0045] According to a further optimized solution, the matrix sampling port 4 is located on the side wall of the main body 5 and is provided with a detachable cover 12 to prevent leakage of the internal matrix and sewage; during the experiment, the cover 12 can be removed to take samples.

[0046] According to a further optimization scheme, any end of the rotating shaft 15 is arranged on a crank 14 which is transmission-connected to the rotating shaft 15 , so as to facilitate the rotation of the rotating shaft 15 .

[0047] According to a further optimization scheme, a prismatic connecting groove is provided at the end of the crank 14 of the present embodiment, and connecting heads adapted to the connecting groove are provided at both ends of the rotating shaft 15. The connecting heads are inserted into the connecting groove when in use and can be removed when not in use, which is flexible and convenient.

[0048] In a further optimized solution, activated sludge is inoculated in the matrix in the main body 5. The activated sludge is inoculated in the matrix so that the bacterial colonies in the activated sludge can spread throughout the matrix and multiply, playing an important role in the degradation of pollutants in sewage.

[0049] Experimental steps:

[0050] S01: The main body 5 and the base 7 are connected by rotation through the connecting thread 6, and the substrate required for the experiment is filled from bottom to top in sequence, and the aquatic plants 1 required for the experiment are planted on the surface of the substrate. Finally, activated sludge is added, and microorganisms related to the degradation of pollutants are cultivated to attach to the surface of the substrate to degrade pollutants in the sewage. When the microbial community in the artificial wetland is stable, the actual sewage can be introduced into the model for sewage treatment and purification;

[0051] S02: After the initial culture of activated sludge is completed, the sewage used in the experiment is added to completely immerse the substrate for the experiment. If the dynamic changes of the microbial community attached to the substrate surface need to be studied in the middle of the experiment, the cover can be unscrewed and tweezers or a medicine spoon can be used to perform non-destructive sampling at the corresponding substrate sampling port 4 to meet the target substrate required by the experiment; in addition, different layers of substrate-purified sewage can be collected in situ at different substrate sampling ports 4 to explore the efficiency of sewage purification by different substrate layers;

[0052] S03: At the end of the experiment, the simulation container is laid flat in the semi-cylindrical groove 9, the connecting thread 6 is rotated to separate the main body 5 and the base 7, and then the hook 19 is pulled to hang it on the handle 3, the crank 14 is turned to drive the rotating shaft 15 to rotate, and the rotating shaft 15 drives the two rope collectors 11 to retract the extended connecting rope 18, and the simulation container is pulled toward the sealing plate 13 by the hook 19 hooked on the end of the connecting rope 18, and the push plate 20 pushes the matrix out of the open top of the simulation container, and the internal planed surface of the matrix will appear in the semi-cylindrical groove 9, so that the target matrix can be accurately collected for analyzing the dynamic changes of the microbial community;

[0053] S04: After the sampling is completed, the main body 5 and the base 7 are connected again by rotating the thread, and can be reused for subsequent experiments.

[0054] A 120-day control experiment was carried out using the above-mentioned artificial wetland model and the traditional artificial wetland model. The two models were identical in size, shape and size except for their different structures. At the end of the 120th day experiment, the artificial wetland model of the present invention used auxiliary tools to collect one sample of the upper, middle and lower matrix, for a total of 3 samples. The traditional artificial wetland model collected a total of 3 samples of the upper, middle and lower matrix by hand, and the collection sites and quality of the matrix of the two models were the same. Then, the samples collected from the two models were stored at -20°C, and DNA was extracted using the DNeasy Power Soil kit. The V3-V4 region of the bacterial 16S rRNA gene was selectively amplified, and high-throughput sequencing was performed on the Illumina NovaSeq platform.

[0055] By attaching Figure 5 It can be seen that the three matrix samples collected by the artificial wetland model of the present invention are significantly separated along the main coordinate axes 1 and 2; on the contrary, the three samples collected by the traditional artificial wetland model overlap in the figure. These results show that the differences between the matrix samples collected by the traditional artificial wetland model are small. This may be because the upper matrix slides to the middle layer or the lower layer during the manual collection process, resulting in the upper matrix being mixed in the collected samples, resulting in small differences in the microbial communities attached to the matrix in the upper, middle and lower layers. The artificial wetland model of the present invention can avoid this phenomenon, greatly reduce the experimental errors caused in the process of matrix collection, and can more realistically reflect the dynamic changes of the microbial communities attached to the matrix, making the experimental data more real and reliable, and providing reliable support for subsequent data analysis.

[0056] In the description of the present invention, it should be understood that the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside" and "outside" etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0057] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A substrate precision collection device for a vertical flow artificial wetland model, characterized in that: It includes a simulation mechanism for simulating a vertical flow artificial wetland, and the simulation mechanism is equipped with an auxiliary mechanism for performing accurate sampling after the experiment; The simulation mechanism comprises a simulation container, the simulation container is filled with a matrix, and a plurality of matrix sampling ports (4) are longitudinally arranged on the side wall of the simulation container to facilitate the collection of matrix samples during the simulation experiment process; The auxiliary mechanism comprises a semi-cylindrical groove (9) adapted to the simulation container, the main body of the simulation container lies horizontally in the semi-cylindrical groove (9), and an ejection component is arranged at the bottom end of the semi-cylindrical groove (9), and the ejection component abuts against the end of the matrix in the simulation container, and is used to push out the matrix in the simulation container after the simulation experiment is completed.

2. The substrate precision collection device for the vertical flow artificial wetland model according to claim 1 is characterized in that: The ejection assembly comprises a sealing plate (13) fixedly connected to the end of the semi-cylindrical groove (9), a fixed push rod (10) facing the inner cavity of the semi-cylindrical groove (9) is fixedly connected to the sealing plate (13), and a push plate (20) is fixedly connected to one end of the fixed push rod (10) away from the sealing plate (13), and the push plate (20) abuts against the bottom end of the matrix in the simulation container to push the matrix out of the simulation container.

3. The substrate precision collection device for the vertical flow artificial wetland model according to claim 2 is characterized in that: The ejection assembly comprises a rotating shaft (15) rotatably connected to the bottom end of the semi-cylindrical groove (9), the two ends of the rotating shaft (15) respectively extend out of the semi-cylindrical groove (9) and are provided with a rope collector (11), a connecting rope (18) is wound around the rope collector (11), and a hook (19) for fixing the simulation container is provided on the connecting rope (18).

4. The substrate precision collection device for the vertical flow artificial wetland model according to claim 3 is characterized by: Two symmetrically arranged handles (3) are provided at the top of the side wall of the simulation container, and the hook (19) is hooked on the handle (3). The crank (14) is rotated to drive the rope collector (11) to rotate and push out the matrix in the simulation container.

5. The substrate precision collection device for the vertical flow artificial wetland model according to claim 1 is characterized in that: The simulation container comprises a main body (5) for filling a matrix, and the matrix sampling port (4) is arranged on the side wall of the main body (5); the bottom end of the main body (5) is connected to a base (7) with a bottom sealing via a thread (6).

6. The substrate precision collection device for the vertical flow artificial wetland model according to claim 5 is characterized by: The base (7) is provided with a sewage inlet (8), and the top side wall of the main body (5) is provided with an outlet (2) for discharging purified sewage.

7. The substrate precision collection device for the vertical flow artificial wetland model according to claim 5, characterized in that: The top of the main body (5) is open and is planted with aquatic plants (1).

8. The substrate precision collection device for the vertical flow artificial wetland model according to claim 5, characterized in that: The matrix sampling port (4) is located on the side wall of the main body (5) and is provided with a detachable cover (12). When sampling, the cover (12) can be removed to take the sample.

9. The substrate precision collection device for the vertical flow artificial wetland model according to claim 3, characterized in that: Any end of the rotating shaft (15) is arranged on a crank (14) which is transmission-connected to the rotating shaft (15) to facilitate the rotation of the rotating shaft (15).

10. The substrate precision collection device for the vertical flow artificial wetland model according to claim 5, characterized in that: The matrix in the main body (5) is inoculated with activated sludge from a sewage treatment plant.

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