LED light formula for synergistically improving resistance, yield and quality of anoectochilus formosanus and application thereof

Abstract

The invention relates to the technical field of medicinal plant cultivation, in particular to an LED light formula for synergistically improving resistance, yield and quality of anoectochilus formosanus and application of the LED light formula. The transplanted anoectochilus formosanus is treated by using LED blue light, red light and far-red light groups, the antioxidant enzyme activity and lignin content of plants of the treatment group are remarkably improved compared with those of a control group, the stem rot disease progress is delayed, and the morbidity and disease index are obviously reduced; the LED light formula provided by the invention has a stable yield effect in the production of anoectochilus formosanus, and is beneficial to increasing the yield of dry anoectochilus formosanus; the LED light formula provided by the invention is beneficial to accumulation of effective active ingredient polysaccharide in anoectochilus formosanus, and can promote deep development of anoectochilus formosanus polysaccharide products. Synergistic improvement of anoectochilus formosanus disease resistance, biomass accumulation and polysaccharide content can be achieved.

Description

Technical Field

[0001] The invention relates to the technical field of medicinal plant cultivation, and in particular to an LED light formula for synergistically improving the resistance, yield and quality of golden thread lily and an application thereof. Background Art

[0002] Anoectochilus roxburghii (Wall.) Lindl. is a precious and rare medicinal plant with medicinal, edible and ornamental value. It is rich in polysaccharides, flavonoids, sterols and other chemically active ingredients, and has the effects of cooling and reducing fire, calming the liver and detoxifying, nourishing and strengthening. Due to the destruction of the natural environment, excessive excavation, low seed germination rate, slow growth and development, etc., wild Anoectochilus resources have been included in the "National Secondary Protected Plant List". In recent years, the demand for Anoectochilus in the domestic and foreign markets has continued to rise. Artificial cultivation is the only way to protect wild Anoectochilus resources and promote the sustainable development of the Anoectochilus industry. At present, the main cultivation methods of Anoectochilus are facility cultivation and under-forest simulated wild cultivation mode, and most seedlings are tissue culture seedlings. However, due to the organizational characteristics of tissue culture seedlings and strict requirements on environmental factors, there are problems such as "weak disease resistance, unstable yield, and reduced quality" in the artificial cultivation of Anoectochilus. Therefore, creating an environment suitable for the growth of Anoectochilus after transplanting is the key to successful cultivation.

[0003] As one of the most important environmental factors affecting plant growth and development, light is an element that must be considered in artificial cultivation of golden thread vine. Wild golden thread vines mostly grow under the shade of forests with a shade degree of 60-80%. Accordingly, Niu Huan et al. (2020) used shade nets or artificial light sources to weaken or control the light intensity reaching the golden thread vine canopy, which optimized the cultivation environment of golden thread vine to a certain extent. However, these studies or practices ignored the effect of the proportion of different wavelength spectrums in the light on the growth of golden thread vine after transplanting. Exploring the optimal spectrum formula suitable for golden thread vine after transplanting is of great significance for the disease resistance, high yield and high-quality cultivation of golden thread vine. Summary of the invention

[0004] The technical problem to be solved by the present invention is to provide an LED light formula for synergistically improving the resistance, yield and quality of golden thread vine in cultivation.

[0005] In order to solve the above technical problems, the technical solution adopted by the present invention is:

[0006] The LED light formula that synergistically improves the resistance, yield and quality of golden thread vine has a ratio of blue light irradiance to red light irradiance of 1.45-1.55, and a ratio of red light irradiance to far-red light irradiance of 0.47-0.50.

[0007] Furthermore, in the LED light formula for synergistically improving the resistance, yield and quality of golden thread vine, the irradiance in the range of 400-700nm is 12-14W·m -2 , photosynthetic photon flux density is 50-60 μmol·m -2 ·s -1 , the far-infrared light irradiance in the range of 701-800nm ​​is 10-12W·m -2 , the far-infrared photon flux density is 60-70 μmol·m -2 ·s -1 .

[0008] Furthermore, in the LED light formula for synergistically improving the resistance, yield and quality of golden thread vine, the blue light irradiance in the range of 400-500nm is 7.83W·m -2 The red light irradiance in the range of 601-700nm is 5.35W·m -2 , the ratio of blue light irradiance to red light irradiance is 1.46.

[0009] Furthermore, in the LED light formula for synergistically improving the resistance, yield and quality of golden thread vine, the red light irradiance in the range of 601-700nm is 5.35W·m -2 The far-infrared irradiance in the range of 701-800nm ​​is 11.0W·m -2 , the ratio of red light irradiance to far-red light irradiance is 0.49.

[0010] The present invention also relates to the application of an LED light formula for synergistically improving the resistance, yield and quality of roxburghii in a roxburghii cultivation method. The roxburghii cultivation method comprises: after hardening the roxburghii tissue culture seedlings, transplanting them into soil, the cultivation temperature is 22-24° C., the relative humidity is 70-80%, and the lighting conditions adopt the above-mentioned LED light formula.

[0011] The present invention also protects a golden thread vine product obtained by cultivation under the above-mentioned LED light formula conditions that synergistically improve the resistance, yield and quality of golden thread vine, which has high antioxidant enzyme activity, high lignin content, good stem rot resistance and high polysaccharide content.

[0012] The present invention also protects a roxburghii product cultivated by the roxburghii cultivation method, which has high antioxidant enzyme activity, high lignin content, good stem rot resistance and high polysaccharide content.

[0013] The beneficial effects of the present invention are as follows: the present invention uses different LED blue light, red light, and far-red light combination light formulas to treat the transplanted golden thread vine, and the antioxidant enzyme activity and lignin content of the plants in the treatment group are significantly improved compared with the control group, the onset of stem rot is delayed, the incidence rate and disease index are significantly reduced, and the disease prevention effect reaches 52.3%, indicating that the LED light formula proposed by the present invention can effectively prevent and control the occurrence and development of stem rot in the production of golden thread vine; compared with the control group, the biomass accumulation of the plants in the leaves and stems of the treatment group increased by 1.70-24.9% to varying degrees, and the dry rate was significantly increased by 11.6-16.5%, indicating that the LED light formula proposed by the present invention has a stable yield effect in the production of golden thread vine and helps to increase the dry product yield of golden thread vine; after growing in the treatment group for 4 months, the polysaccharide content of the leaves and stems of the golden thread vine plants increased by 12.2-17.4% compared with the control group, indicating that the LED light formula proposed by the present invention helps to accumulate polysaccharides, an effective active ingredient in golden thread vine, and can promote the in-depth development of golden thread vine polysaccharide products. The present invention can achieve the coordinated improvement of disease resistance, biomass accumulation and polysaccharide content of golden thread vine, lay an application foundation for the future batch production of highly resistant, high-yield and high-quality golden thread vine, and provide new ideas for the optimization of golden thread vine cultivation technology and environmental control measures. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1a This is a diagram of the experimental environment for light treatment in the method for cultivating roxburghii in Example 1 of the present invention;

[0015] Figure 1b This is a diagram of a controller panel of a light processing device in a method for cultivating roxburghii in Example 1 of the present invention;

[0016] Figure 2a This is the LED spectrum distribution diagram of the CK group in the light treatment in the roxburghii cultivation method of Example 1 of the present invention;

[0017] Figure 2b This is a LED spectrum distribution diagram of the FR group in the light treatment in the roxburghii cultivation method of Example 1 of the present invention;

[0018] Figure 3a This is a bar graph of the peroxidase activity measurement results of the roxburghii roxburghii plants treated with the CK group and the FR group in Example 1 of the present invention;

[0019] Figure 3b This is a bar graph of the superoxide dismutase activity measurement results of the Anoectochilus roxburghii plants treated with the CK group and the FR group in Example 1 of the present invention;

[0020] Figure 4a This is a bar graph showing the results of measuring the lignin content of the Anoectochilus roxburghii plants after being treated with the CK group and the FR group in Example 1 of the present invention;

[0021] Figure 4bThis is a diagram showing the results of measuring lignin distribution of the roxburghii roxburghii plants after being treated with the CK group and the FR group in Example 1 of the present invention;

[0022] Figure 5a It is a plan view of the roxburghii roxburghii plants after being treated with the CK group and the FR group in Example 1 of the present invention;

[0023] Figure 5b It is a top view of the roxburghii plants after being treated with the CK group and the FR group in Example 1 of the present invention;

[0024] Figure 5c This is a schematic diagram of the rhizome junction of the roxburghii roxburghii plants after being treated with the CK group and the FR group in Example 1 of the present invention;

[0025] Figure 6a The biomass bar graph of the roxburghii plants after being treated with the CK group and the FR group in Example 1 of the present invention;

[0026] Figure 6b This is a bar graph of the biomass of the leaves of Anoectochilus roxburghii after being treated with the CK group and the FR group in Example 1 of the present invention;

[0027] Figure 6c This is a bar graph of the biomass of the stems of Anoectochilus roxburghii after being treated with the CK group and the FR group in Example 1 of the present invention;

[0028] Figure 6d It is a bar graph of the dryness ratio of Anoectochilus roxburghii after being treated with the CK group and the FR group in Example 1 of the present invention;

[0029] Figure 7 This is a bar graph of the polysaccharide content of Anoectochilus roxburghii after treatment in the CK group and the FR group in Example 1 of the present invention. DETAILED DESCRIPTION

[0030] In order to explain the technical content, achieved objectives and effects of the present invention in detail, the following is an explanation in conjunction with the implementation modes and the accompanying drawings.

[0031] LED is the abbreviation of light emitting diode. It has the characteristics of small size, light weight, long life and low energy consumption. In particular, the light quality of LED is pure and specific, and the spectral distribution is stable and easy to adjust. Therefore, it has become the preferred artificial light source in plant growth research.

[0032] In the present invention, blue light refers to LED light with a wavelength range of 400 to 500 nm, specifically 450 nm, represented by "B"; red light refers to LED light with a wavelength range of 601 to 700 nm, specifically 660 nm, represented by "R"; far-infrared light refers to LED light with a wavelength range of 701 to 800 nm, specifically 730 nm, represented by "FR".

[0033] Unless otherwise specified, the test methods used in the following examples are conventional methods; the materials, reagents, etc. used are commercially available materials and reagents unless otherwise specified. The plant light analyzer used in the present invention is PLA-30, purchased from Hangzhou Yuanfang Optoelectronic Information Co., Ltd.; the LED light source used is a 10-channel integrated chip light board covering ultraviolet / blue / green / yellow / red / far-red bands, provided by the Photoelectronic Agricultural Engineering and Technology Research Center of Fujian Agriculture and Forestry University, and the irradiance of each color LED can be controlled by a digital controller.

[0034] Example 1

[0035] Aromatic thread vine cultivation methods that synergistically improve the resistance, yield and quality of Aromatic thread vine, including LED light treatment;

[0036] In this embodiment, a CK group and a FR group are set. The CK group refers to a control group set to simulate the ratio of different spectral bands in natural light, and the FR group refers to an experimental group set with the improved spectral band ratio of the present invention; the preset light conditions of the CK group and the FR group are achieved by adjusting the digital controller panel that communicates with the integrated chip light board. In order to avoid the influence of light sources of different treatments, each treatment is a cubic compartment, with five sides blocked by aluminum foil reflective plates and one side blocked by aluminum foil reflective cloth, which is convenient for observing and sampling the growth status of the plants. Figure 1a and Figure 1b The light source was set directly above the plant, and the irradiance in the range of 400-700 nm at the canopy of the plant was measured by a plant light analyzer to be 12-14 W·m -2 , photosynthetic photon flux density is 50-60 μmol·m -2 ·s -1 , LED spectrum distribution is as follows Figure 2a and Figure 2b The average values ​​of the optical parameters are shown in Table 1.

[0037] Table 1

[0038]

[0039] In the preferred light recipe, the photosynthetic photon flux in the range of 400-700 nm is 59.4 μmol·m -2 ·s -1 , blue light irradiance is 7.83W·m -2 , the red light irradiance is 5.35W·m -2 , the far-red photon flux density in the range of 701-800 nm is 67.5 μmol·m -2 ·s -1 , far-infrared light irradiance is 11.0W·m -2, the irradiance ratio of blue light and red light is 1.46, and the irradiance ratio of red light and far-red light is 0.49.

[0040] The cultivation and management conditions of the CK group and the FR group are the same except that the illumination conditions are different. In this embodiment, the 6-month-old tissue culture seedlings of "Hongxia" are selected as test materials. The tissue culture seedlings with basically the same shape and size and strong are selected and planted in a cultivation tray (40cm long × 40cm wide × 8cm high) after cleaning, disinfection and hardening and placed in the above-mentioned LED light treatment, with a photoperiod of 12h light / 12h dark, an ambient temperature of 22-24°C, and a relative humidity of 70-80% for 4 months in a facility culture room. All plants are watered with tap water once every 3 days and Huabao fertilizer No. 2 once every 15 days. After the treatment period, plant samples are taken to measure relevant indicators.

[0041] In the embodiments, "PPFD" refers to photosynthetic photon flux density (unit: umol · s -1 · m -2 ), "E" refers to irradiance (Phytometric irradiance, unit is W · m -2 ), with a wavelength range of 400 to 700 nm; “PPFDF” refers to the far red photon flux density (unit: umol · s -1 · m -2 ), “EF” refers to far red irradiance (unit: W) · m -2 ), wavelength range is 701~800nm; “ER” refers to red-orange irradiance (unit is W · m -2 ), the wavelength range is 601~700nm; “EB” refers to blue-purple irradiance (in W · m -2 ), the wavelength range is 400 ~ 500nm; "R / FR" refers to the ratio of red light irradiance (ER, 601 ~ 700nm) to far-red light irradiance (EF, 701 ~ 800nm).

[0042] The measurement of some traits in the following experimental examples refers to the provisions of the Fujian Provincial Local Standard "Geographical Indication Product Yong'an Golden Thread Lotus" (DB35 / T 1388-2013).

[0043] Most of the measurement methods used are: individual measurement and group visual inspection. The tools used for individual measurement include but are not limited to ruler, vernier caliper, electronic balance, and spectrophotometer. Some indicators have been marked with methods. Six individual plants that best represent the growth status of the overall treatment plants under the CK group and FR group were selected for determination of relevant indicators.

[0044] Experimental Example 1

[0045] The antioxidant enzyme activity of the roxburghii plants treated with the CK group and the FR group in Example 1 was determined by the following method:

[0046] Extraction of enzyme solution: Take 0.5g of fresh stem and leaf samples, add 5.0mL of 50mM phosphate buffer (pH7.8, containing 1mM ethylenediaminetetraacetic acid, 0.05% Triton X-100 and 2% polyvinylpyrrolidone) three times, grind in liquid nitrogen until homogenate. Transfer the homogenate to a 10ml centrifuge tube and centrifuge at 4℃ and 12000rpm. · min -1 The mixture was centrifuged for 20 min, and the supernatant was collected for determination of peroxidase POD and superoxide dismutase SOD activities;

[0047] Determination of peroxidase POD activity: 20ul of supernatant was added to 3ml of reaction solution containing 100mM phosphate buffer (pH 6.0), guaiacol and 30% hydrogen peroxide. The reaction solution was read 5 times at 470nm with an interval of 30 seconds, and the POD activity was expressed as the absorbance change value per minute.

[0048] Determination of superoxide dismutase (SOD) activity: 20ul of supernatant was added to 3ml of reaction solution containing 50mM sodium phosphate buffer (pH 7.8), 130mM methionine, 0.75mM nitroblue tetrazolium, 0.1mM ethylenediaminetetraacetic acid and 0.02mM riboflavin. The mixture was placed under a 4000lx white fluorescent light for 20min and then the SOD activity was determined at a wavelength of 560nm. The above determination results are shown in Figure 2. Figure 3a and Figure 3b As shown, the light-colored columns are CK and the dark-colored columns are FR.

[0049] The results showed that the POD activity of the FR group was higher in both leaves and stems than that of the CK group, and there was a significant difference in the stems (P<0.05) ( Figure 3a ); Similarly, the SOD activity in the FR group was increased by 39.9% and 31.1% in leaves and stems, respectively, compared with the CK group, and the increase in leaves reached a very significant level (P<0.01) ( Figure 3b). POD and SOD can effectively remove reactive oxygen free radicals and are an important part of the plant defense response mechanism. The FR group increased the antioxidant enzyme activity of Anoectochilus roxburghii plants, which helped them survive under stress.

[0050] Experimental Example 2

[0051] The content and distribution of lignin in the roxburghii plants treated with the CK group and the FR group in Example 1 were measured by the following method:

[0052] Determination of lignin content: Weigh 0.005g of crushed dried plant sample and place it in a 2ml EP tube. Set a blank tube without sample. Add 500μL of 30% (v / v) acetyl bromide glacial acetic acid solution and 20μL of 0.69M perchloric acid to the sample tube and the blank tube. Mix well and seal. Place in a constant temperature water bath at 80℃ for 40min to carry out sufficient acetylation reaction. Then take out and cool naturally to room temperature. Add 250μL of 2M NaOH and 250μL of glacial acetic acid solution to terminate the reaction. Mix well and heat at room temperature and 12000rpm. · min -1 Centrifuge for 10 min, take 10 μL of the supernatant and add 990 μL of glacial acetic acid to dilute to 1 mL. After thorough mixing, use a 1 mL quartz cuvette to measure the absorbance at a wavelength of 280 nm using an ultraviolet spectrophotometer to calculate the lignin content.

[0053] Positioning of lignin distribution: Select fresh plants of Anoectochilus roxburghii, manually cut a 5mm stem segment from the second internode at the junction of the rhizome, fix it with FAA fixative for more than 48 hours, dehydrate and make transparent with gradient ethanol solution and gradient xylene solution, embed it with pure wax with a melting point of 56-58°C, slice it with Leica automatic slicer (thickness 10μm), spread it in a 40°C water bath, bake it in a 38°C oven for more than 72 hours, and set aside. Place the slices on a glass slide, add a few drops of 2% phloroglucinol ethanol solution to stain for 3 minutes, then add a few drops of 3 mol / L hydrochloric acid and stain for 1 minute. After sealing, observe, that is, stain it with purple-red using the Wiesner method to show the location of G-lignin. The above measurement results are as follows Figure 4a and Figure 4b shown.

[0054] The results showed that compared with the CK group, the FR group increased the synthesis and accumulation of lignin in the leaves and stems of Anoectochilus roxburghii plants, with the stems reaching a highly significant difference at the 0.01 level (P<0.01) ( Figure 4a ); Phloroglucinol staining and microscopic observation results showed that compared with the CK group, the FR group promoted the deposition of lignin in the stem nodes ( Figure 4b). Lignin is an important secondary metabolite of plants, an important component of cell walls and a signature product of defense response. The FR group increased the lignin content and deposition of A. roxburghii plants, which helped to enhance the host's defense capabilities.

[0055] Experimental Example 3

[0056] The roxburghii plants treated with the CK group and the FR group in Example 1 were tested for their stem rot resistance, and the test method was as follows:

[0057] Pathogen inoculation: The method of root irrigation with Fusarium oxysporum (the pathogen of stem rot) suspension was used. Specifically, the pathogen was cultured on solid PDA medium at 28°C for 7 days, and then the pathogen cakes of uncontaminated and suitable strains were selected and transferred to liquid PDA medium (5 mm in diameter, 6 to 7 pieces / 100 mL) using a hand-held puncher. The culture was placed at 28°C and 150 rpm. · min -1 Culture in a shaker for 3 to 5 days. Observe that the bacterial solution turns purple-red, filter it, and add sterile distilled water to dilute it appropriately. Calculate the concentration of 1×10 6 Use a pipette to infuse the prepared pathogen suspension into the root-stem junction of the test plant, add 15 mL of suspension to each plant, and use sterilized liquid PDA as a control group. Record the disease situation after inoculation.

[0058] Evaluation of disease index of golden thread rot disease: 30 days after inoculation of pathogens, the diseased plants were counted, and the golden thread rot disease was recorded according to the disease classification standard (Table 2), and the incidence rate, disease index and disease prevention effect were calculated.

[0059] Table 2 Disease levels and corresponding symptoms of Anoectochilus roxburghii

[0060]

[0061] Incidence rate (%) = diseased plants / total number of plants surveyed × 100

[0062] Disease index (%) = Σ(disease level × number of plants at that level) / (total number of plants × most severe disease level) × 100

[0063] Disease prevention effect (%) = (disease index of CK group - disease index of FR group) / disease index of CK group × 100

[0064] The measurement results are shown in Table 3:

[0065] Table 3 Incidence of stem rot in Anoectochilus roxburghii plants

[0066]

[0067] The results showed that compared with the CK group, the FR group delayed the onset of disease. Figure 5a , Figure 5b and Figure 5c The incidence rate decreased by 23.4%, the disease index decreased by 37.7%, and the disease prevention effect reached 52.3%.

[0068] Experimental Example 4

[0069] The overall growth-related traits of the CK group and FR group treated in Example 1 were measured, including: plant height: the height from the base of the plant rhizome to the top bud; stem diameter: the diameter of the middle position of the second stem segment from the base upward; number of leaves: a leaf with more than 1 / 2 unfolded is counted as one leaf; leaf area: the leaves of the whole plant, each leaf is calculated according to leaf length (excluding the part of the petiole, the vertical distance from the bottom to the highest point) × leaf width (the longest distance from the outermost edge of the left side of the leaf to the outermost edge of the right side) × 0.75. The above measurement results are shown in Table 4.

[0070] Table 4 Statistical table of plant overall growth related traits

[0071]

[0072] The above results showed that there was no significant difference in the overall growth of plants between the CK group and the FR group, and the Anoectochilus roxburghii plants did not show shade avoidance response under the low R / FR conditions of the FR group, which contributed to the synergistic improvement of growth and defense.

[0073] Experimental Example 5

[0074] The biomass accumulation of the roxburghii plants treated with the CK group and the FR group in Example 1 was measured by the following method:

[0075] Each plant was divided into stems and leaves by hand, and the fresh weight was weighed with an electronic balance, then placed in a paper bag and fixed at 105℃ for 10 minutes, and then placed at 70℃ to dry until constant weight, in order to determine the biomass accumulation of different parts, and calculate the dry weight (%) = (plant dry weight / plant fresh weight) × 100. The results showed that the fresh and dry weight of the whole plant, leaves and stems increased to a certain extent in the FR group, but did not reach a significant level (P>0.05); compared with the CK group, the FR group increased the dry weight of leaves and stems, and the dry weight of leaves reached a significant level (P<0.05), referring to Figure 6a , Figure 6b , Figure 6c and Figure 6d , where the light-colored columns are CK and the dark-colored columns are FR.

[0076] The above results indicate that the FR group has the effect of stabilizing the plant growth morphology and biomass accumulation in the production of A. roxburghii, and helps to increase the yield of dry products of A. roxburghii.

[0077] Experimental Example 6

[0078] The polysaccharide content of the active ingredient in the CK group and FR group treated plants of Anoectochilus roxburghii in Example 1 was determined as follows:

[0079] 0.1 g of crushed dried plant sample was extracted in 8 ml of deionized water (100 °C) for 20 min. After cooling, the mixture was heated at room temperature and 4500 rpm. · min -1 Centrifuge for 10 minutes (repeat 3 times), combine the supernatant containing sugars and make up to a total volume of 50 ml with deionized water. Take 1 ml of the supernatant, use the sulfuric acid-anthrone method at a wavelength of 625 nm to determine and calculate the soluble sugar content, and use the 3,5-dinitrosalicylic acid (DNS) colorimetric method at a wavelength of 540 nm to determine and calculate the reducing sugar content. Polysaccharide content = soluble sugar content - reducing sugar content. The results showed that compared with the CK group, the polysaccharide content in the leaves of Anoectochilus roxburghii increased by 12.2% and in the stems by 17.4% under the FR group, indicating that the FR group contributes to the accumulation of polysaccharide content in Anoectochilus roxburghii, which is more obvious in the stems, refer to Figure 7 , where the light-colored column is CK and the dark-colored column is FR.

[0080] The above descriptions are merely embodiments of the present invention and are not intended to limit the patent scope of the present invention. Any equivalent transformations made using the contents of the present invention's specification and drawings, or directly or indirectly applied in related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. An LED light formula that synergistically improves the resistance, yield and quality of golden thread vine, characterized in that: The ratio of blue light irradiance to red light irradiance is 1.45-1.55, and the ratio of red light irradiance to far-red light irradiance is 0.47-0.

50.

2. The LED light formula for synergistically improving the resistance, yield and quality of Anoectochilus roxburghii according to claim 1, characterized in that: The irradiance in the range of 400-700 nm in the light formula is 12-14 W·m -2 The photosynthetic photon flux density in the range of 400 to 700 nm in the light recipe is 50 to 60 μmol·m -2 ·s -1 .

3. The LED light formula for synergistically improving the resistance, yield and quality of Anoectochilus roxburghii according to claim 1, characterized in that: The blue light irradiance in the range of 400-500nm is 7.83W·m -2 The red light irradiance in the range of 601-700nm is 5.35W·m -2 , the ratio of blue light irradiance to red light irradiance is 1.

46.

4. The LED light formula for synergistically improving the resistance, yield and quality of Anoectochilus roxburghii according to claim 1, characterized in that: The red light irradiance in the range of 601-700nm is 5.35W·m -2 The far-infrared irradiance in the range of 701-800nm ​​is 11.0W·m -2 , the ratio of red light irradiance to far-red light irradiance is 0.

49.

5. The application of LED light formula for synergistically improving the resistance, yield and quality of golden thread vine in the golden thread vine cultivation method is characterized in that: The method for cultivating roxburghii comprises: after hardening, the roxburghii tissue culture seedlings are transplanted into soil, the cultivation temperature is 22-24° C., the relative humidity is 70-80%, and the lighting condition adopts the LED light formula described in any one of claims 1 to 4.

6. A golden thread vine product having high antioxidant enzyme activity, high lignin content, good stem rot resistance and high polysaccharide content obtained by cultivating under the conditions of the LED light formula for synergistically improving the resistance, yield and quality of golden thread vine as described in any one of claims 1 to 4.

7. A roxburghii product cultivated by the roxburghii cultivation method of claim 5, which has high antioxidant enzyme activity, high lignin content, good stem rot resistance and high polysaccharide content.

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