Collagen enhanced skin care composition for resisting aging

Abstract

The invention discloses an anti-aging collagen enhanced skin care composition, relates to the technical field of skin care, and adopts the technical scheme that the composition comprises a core effective component and an auxiliary component, the core component comprises resveratrol and sophoricoside, and the auxiliary component comprises an auxiliary component and an auxiliary component; the auxiliary components comprise active collagen, hyaluronic acid, trehalose, vitamin C or a derivative thereof, phenoxyethanol, carbomer, an emulsifier, glycerol and the balance of pure water. By using the specific emulsifier and accurately controlling the pH value, the stability of resveratrol and sophoricoside and the absorption rate of resveratrol and sophoricoside on skin are remarkably improved; the active ingredients can more effectively permeate into the deep layer of the skin, and the anti-aging effect is achieved. Through scientific proportioning of the two components, the synergistic effect is optimized, the anti-oxidation and anti-inflammatory efficacy is remarkably improved, the capacity of the formula for resisting external environment pressure is enhanced, and skin aging is effectively delayed. The optimized auxiliary components improve the moisture retention and skin feeling of the product, so that the emulsion is easy to absorb.

Description

Technical Field

[0001] The invention relates to the technical field of skin care, and in particular to a collagen-enhanced skin care composition for anti-aging. Background Art

[0002] The field of anti-aging skin care has been exploring and developing formulations with innovative and highly effective active ingredients to address the multifaceted challenges of skin aging. Although there are a variety of products on the market containing classic ingredients (such as vitamin C, hyaluronic acid, etc.), researchers and product developers are still looking for new solutions to provide more effective antioxidant and anti-inflammatory effects. In this context, resveratrol has been widely studied for its excellent antioxidant properties. At the same time, sophora flavescens, a natural extract rarely used in skin care products, has potentially powerful biological activity that has not been fully utilized, especially its possible synergistic effect with resveratrol.

[0003] Existing anti-aging skin care products mostly focus on the efficacy of single or traditional ingredients, while ignoring the synergistic effects achieved through innovative combinations of potential ingredients. In particular, research on sophora japonica glycosides is relatively rare in the anti-aging field, and its synergistic effect in combination with resveratrol has not been reported in existing literature and products. In addition, existing products often face problems such as insufficient stability of active ingredients, low skin absorption rate and poor long-term effectiveness. These limitations affect the overall efficacy of anti-aging products, making many solutions on the market unable to fully meet consumers' demand for efficient and long-lasting skin care effects.

[0004] The present invention is dedicated to developing an anti-aging skin care composition that combines resveratrol and sophora japonica glycosides. This combination utilizes the synergistic effect of the two ingredients to achieve an anti-aging effect that is superior to the use of each ingredient alone. The key technical problems that the present invention needs to solve include how to effectively combine the two ingredients to improve their stability and skin absorbability, and how to maximize their synergistic effect through scientific formula design. Through this technological innovation, it is expected to solve the deficiencies of existing products in terms of anti-aging effects, ingredient stability and user experience, thereby providing a new and more effective anti-aging skin care solution. Summary of the invention

[0005] In order to achieve the above-mentioned purpose of the invention and to solve the above-mentioned technical problems, the present invention provides a collagen-enhanced skin care composition for anti-aging, the composition comprising a core active ingredient and auxiliary ingredients:

[0006] The core ingredients include resveratrol and sophora flavescens,

[0007] The auxiliary ingredients include active collagen, hyaluronic acid, trehalose, vitamin C or its derivatives, phenoxyethanol, carbomer, emulsifier, glycerin, and the balance is pure water.

[0008] Preferably, the concentration of resveratrol is 100-500 μg / mL, and the concentration of sophora flavescens is 100-500 μg / mL.

[0009] Preferably, the concentration of resveratrol is 200 μg / mL, and the concentration of sophora flavescens is 300 μg / mL.

[0010] Preferably, the auxiliary ingredients include 1-3% active collagen, 0.05-0.2% hyaluronic acid, 0.5-3% trehalose, 0.1-1% vitamin C or its derivatives, 0.5% phenoxyethanol, 0.1-0.5% carbomer, 0.03-0.35% triethanolamine, 1-3% emulsifier, 3-5% glycerol, and the balance is pure water.

[0011] Preferably, the emulsifier is a combination of lecithin and polysorbate.

[0012] Preferably, the mass ratio of lecithin to polysorbate is 1:1-1:2.

[0013] Preferably, the vitamin C derivative is selected from one of ascorbyl tetraisopalmitate, ascorbyl glucoside, magnesium ascorbyl phosphate and sodium ascorbyl phosphate.

[0014] The present invention also provides a method for preparing an anti-aging collagen-enhanced skin care composition, comprising the following steps:

[0015] S1 Add 0.1-0.5% carbomer to pure water, stir until completely mixed, and then add 0.03-0.35% triethanolamine until the carbomer is completely dissolved and forms a uniform colloidal liquid;

[0016] S2 adds 0.5-3% trehalose to the colloidal liquid of S1 in sequence, stirs until completely dissolved, then slowly adds 0.05-0.2% hyaluronic acid, and continues to stir until uniform, to prepare an aqueous phase;

[0017] S3: 1-3% lecithin and polysorbate are mixed with 0.5% phenoxyethanol and 3-5% glycerol in a mass ratio of 1:1 to 1:2, and stirred and heated to 40° C. until a uniform oil phase is formed;

[0018] S4: slowly add the oil phase into the water phase in portions, stirring at high speed at 40-50°C until a uniform emulsion is formed;

[0019] S5: lower the temperature to room temperature, add 1-3% active collagen, 0.1-1% vitamin C or its derivatives in sequence, continue stirring until all active ingredients are evenly dispersed, and adjust the pH value to 5.0-7.0 to obtain auxiliary ingredients;

[0020] S6, under light-proof conditions, adding resveratrol and sophora flavescens to the auxiliary ingredients, wherein the concentration of resveratrol is 50-600 μg / mL, and the concentration of sophora flavescens is 50-600 μg / mL, and stirring until uniformly dispersed;

[0021] S7 ensures the uniformity and stability of the emulsion through vacuum degassing and high-speed homogenization, and finally encapsulates under sterile conditions.

[0022] The present invention also provides an application of the composition in the preparation of anti-aging cosmetics, wherein the composition is applied to emulsions, creams or facial masks.

[0023] The core of the invention lies in the unique combination of resveratrol and sophora flavescens, both of which have significant antioxidant and anti-inflammatory properties. Resveratrol is a natural polyphenol that has been widely studied for slowing cell aging and protecting against free radical damage. Sophora flavescens, on the other hand, is a lesser-used natural extract. The combination of these two ingredients, through synergistic action, provides an anti-aging effect that goes beyond traditional single antioxidants or anti-inflammatory agents.

[0024] The auxiliary ingredients in the present invention, such as activated collagen, hyaluronic acid, trehalose and vitamin C derivatives, all help to support the effects of the core ingredients. Activated collagen strengthens the skin structure, hyaluronic acid and trehalose provide deep moisturizing, and vitamin C derivatives further enhance antioxidant protection. The interaction of these ingredients not only increases the skin's acceptance of the core anti-aging ingredients, but also improves the stability and bioavailability of the overall formula.

[0025] The beneficial effects brought by the technical solution provided by the present invention are:

[0026] The anti-aging skin care composition of the present invention significantly improves the stability of resveratrol and sophora flavescens and their absorption rate on the skin by using a specific emulsifier and accurately controlling the pH value. This method ensures that the active ingredients can more effectively penetrate into the deep layers of the skin to exert their anti-aging effects.

[0027] By scientifically matching resveratrol and sophora flavescens, the present invention optimizes the synergistic effect of the two and significantly improves their antioxidant and anti-inflammatory effects. This combination increases the ability of the formula to resist external environmental pressure and effectively delays skin aging.

[0028] The present invention optimizes the use of auxiliary ingredients in the formula, improves the moisturizing and skin feel of the product, and makes the lotion easily absorbed by the skin without leaving a greasy feeling. The improved user experience and enhanced moisturizing effect jointly improve the anti-aging performance of the product.

[0029] The present invention solves the problems of stability and absorption rate of active ingredients in traditional anti-aging products by optimizing the use of resveratrol and sophora japonica glycosides, while its innovative formula significantly enhances the anti-aging effect and user satisfaction. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 This is the H&E staining microstructural observation of the skin aging degree of mice in the blank control group of the present invention (X200, scale is 100m).

[0031] Figure 2 This is the H&E staining microstructure observation of the skin aging degree of mice in the model group of the present invention (X200, scale is 100m).

[0032] Figure 3 This is the H&E staining microstructure observation of the mouse skin aging degree in Example 1 of the present invention (X200, scale is 100m).

[0033] Figure 4 This is the H&E staining microstructure observation of the aging degree of mouse skin in Comparative Example 1 of the present invention (X200, scale is 100m).

[0034] Figure 5 The aging degree of mouse skin in comparative example 2 of the present invention was observed by H&E staining microstructure (X200, scale is 100

[0035] m). DETAILED DESCRIPTION

[0036] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. Of course, the specific embodiments described here are only used to explain the present invention and are not used to limit the present invention.

[0037] Example 1

[0038] S1 Add 0.3% carbomer to pure water and stir at room temperature for 30 minutes using a magnetic stirrer to ensure complete mixing. Add 0.2% triethanolamine and continue stirring for 60 minutes until the carbomer is completely dissolved and a uniform colloidal liquid is formed.

[0039] S2 Add 2% trehalose to the colloidal liquid of S1 and stir for 40 minutes to ensure complete dissolution. Slowly add 0.15% hyaluronic acid and continue stirring for 30 minutes until a uniform aqueous phase is prepared.

[0040] S3: 2% lecithin and polysorbate 80 were mixed with 0.5% phenoxyethanol and 4% glycerol in a mass ratio of 1:1.5. The mixture was stirred and heated to 40°C for 30 minutes until a uniform oil phase was formed.

[0041] S4: Slowly add the oil phase into the water phase in portions and stir at high speed at 45°C for 60 minutes until a uniform emulsion is formed.

[0042] S5: Lower the temperature to room temperature, add 2% active collagen and 0.5% vitamin C in sequence, and continue stirring for 30 minutes to ensure that all active ingredients are evenly dispersed, adjust the pH value to 6.0, and obtain auxiliary ingredients.

[0043] S6. Add 200 μg / mL resveratrol and 300 μg / mL sophora flavescens to the auxiliary ingredients under light-protected conditions and stir for 60 minutes until all ingredients are evenly dispersed.

[0044] S7 ensures the uniformity and stability of the emulsion through vacuum degassing and high-speed homogenization for 15 minutes, and encapsulates under sterile conditions to ensure the long-term stability and safety of the product.

[0045] Example 2

[0046] The preparation was carried out according to the same preparation method as in Example 1, except that in step S6, the resveratrol was 300 μg / mL and the sophoroside was 200 μg / mL.

[0047] Example 3

[0048] The preparation was carried out according to the same preparation method as in Example 1, except that in step S6, the resveratrol was 300 μg / mL and the sophoroside was 200 μg / mL.

[0049] Example 4

[0050] The preparation was carried out according to the same preparation method as in Example 1, except that in step S6, the resveratrol was 250 μg / mL and the sophoroside was 250 μg / mL.

[0051] Example 5

[0052] The preparation was carried out according to the same preparation method as in Example 1, except that in step S6, the resveratrol was 100 μg / mL and the sophoroside was 400 μg / mL.

[0053] Example 6

[0054] The preparation was carried out according to the same preparation method as in Example 1, except that in step S6, the resveratrol was 400 μg / mL and the sophoroside was 100 μg / mL.

[0055] Example 7

[0056] The preparation was carried out according to the same preparation method as in Example 1, except that in step S3, the mass ratio of lecithin to polysorbate 80 was 1:1.

[0057] Example 8

[0058] The preparation was carried out according to the same preparation method as in Example 1, except that in step S3, the mass ratio of lecithin to polysorbate 80 was 1:2.

[0059] Example 9

[0060] S1 Add 0.1% carbomer to pure water and stir at room temperature for 30 minutes using a magnetic stirrer to ensure complete mixing. Add 0.03% triethanolamine and continue stirring for 60 minutes until the carbomer is completely dissolved and a uniform colloidal liquid is formed.

[0061] S2 Add 3% trehalose to the colloidal liquid of S1 and stir for 40 minutes to ensure complete dissolution. Slowly add 0.2% hyaluronic acid and continue stirring for 30 minutes until a uniform aqueous phase is prepared.

[0062] S3: 1% lecithin and polysorbate 80 were mixed with 0.5% phenoxyethanol and 3% glycerol in a mass ratio of 1:1. The mixture was stirred and heated to 40°C for 30 minutes until a uniform oil phase was formed.

[0063] S4: Slowly add the oil phase into the water phase in portions and stir at 40°C for 60 minutes until a uniform emulsion is formed.

[0064] S5: Lower the temperature to room temperature, add 3% active collagen and 0.1% ascorbyl tetraisopalmitate in sequence, and continue stirring for 30 minutes to ensure that all active ingredients are evenly dispersed, adjust the pH value to 5.0, and obtain auxiliary ingredients.

[0065] S6. Add 200 μg / mL resveratrol and 300 μg / mL sophora flavescens to the auxiliary ingredients under light-protected conditions and stir for 60 minutes until all ingredients are evenly dispersed.

[0066] S7 ensures the uniformity and stability of the emulsion through vacuum degassing and high-speed homogenization for 15 minutes, and encapsulates under sterile conditions to ensure the long-term stability and safety of the product.

[0067] Example 10

[0068] S1 Add 0.51% carbomer to pure water and stir at room temperature for 30 minutes using a magnetic stirrer to ensure complete mixing. Add 0.35% triethanolamine and continue stirring for 60 minutes until the carbomer is completely dissolved and a uniform colloidal liquid is formed.

[0069] S2 Add 0.5% trehalose to the colloidal liquid of S1 and stir for 40 minutes to ensure complete dissolution. Slowly add 0.05% hyaluronic acid and continue stirring for 30 minutes until a uniform aqueous phase is prepared.

[0070] S3: Mix 3% lecithin and polysorbate 80 with 0.5% phenoxyethanol and 5% glycerol in a mass ratio of 1:2. Stir and heat to 40°C for 30 minutes until a uniform oil phase is formed.

[0071] S4: Slowly add the oil phase into the water phase in portions and stir at 40°C for 60 minutes until a uniform emulsion is formed.

[0072] S5: Lower the temperature to room temperature, add 1% active collagen and 1% magnesium ascorbyl phosphate in sequence, and continue stirring for 30 minutes to ensure that all active ingredients are evenly dispersed, adjust the pH value to 7.0, and obtain auxiliary ingredients.

[0073] S6. Add 200 μg / mL resveratrol and 300 μg / mL sophora flavescens to the auxiliary ingredients under light-protected conditions and stir for 60 minutes until all ingredients are evenly dispersed.

[0074] S7 ensures the uniformity and stability of the emulsion through vacuum degassing and high-speed homogenization for 15 minutes, and encapsulates under sterile conditions to ensure the long-term stability and safety of the product.

[0075] Comparative Example 1

[0076] The preparation was carried out in the same manner as in Example 1, except that in step S6, only 500 μg / mL of resveratrol was added.

[0077] Comparative Example 2

[0078] The preparation was carried out in the same manner as in Example 1, except that in step S6, only 500 μg / mL of sophoroside was added.

[0079] Comparative Example 3

[0080] The preparation was carried out in the same manner as in Example 1, except that only 2% lecithin was added in step S3.

[0081] Comparative Example 4

[0082] The preparation was carried out in the same manner as in Example 1, except that only 2% of polysorbate 80 was added in step S3.

[0083] Comparative Example 5

[0084] The preparation was carried out in the same manner as in Example 1, except that the emulsifier in step S3 was 2% PEG-100 stearate.

[0085] Experimental test:

[0086] 1. DPPH free radical scavenging test

[0087] Prepare 0.0178mmol / L DPPH anhydrous methanol solution.

[0088] Add 3 ml of DPPH solution and the test solution into the cuvette, mix them and measure the initial absorbance (A1) at a wavelength of 517 nm;

[0089] After the mixture was placed in the dark at 37°C for 30 minutes, the absorbance value (A2) was measured again;

[0090] Prepare a blank control containing only DPPH solution and measure its absorbance (A0);

[0091] The DPPH free radical scavenging rate was calculated using the formula: DPPH scavenging rate (%) = [1-(A1-A2) / A0] × 100%;

[0092] The measurements were repeated at least three times to ensure the reliability and accuracy of the data.

[0093] 2. ABTS free radical scavenging test

[0094] Mix 7 mmol / l ABTS and 2.45 mmol / l potassium persulfate (final concentration) and let it stand.

[0095] At room temperature, in dark overnight, dilute the generated ABTS solution with anhydrous ethanol to a final experimental concentration of

[0096] When the absorbance at 734 nm is about 0.5, the ABTS working solution is obtained. Different sample solutions and ABTS working solution are added to a stoppered test tube at a ratio of 19:1.

[0097] The reaction was carried out in the dark for 30 minutes, and the absorbance value A1 was measured at 734 nm with anhydrous ethanol to adjust to zero.

[0098] Ethanol replaces ABTS solution to determine the absorbance value A2; then anhydrous ethanol is used to replace the sample solution to determine the absorbance value

[0099] A0.

[0100] 3. Stability test

[0101] Particle size analysis was performed using a laser particle size analyzer.

[0102] Instrument model: Mastersizer 3000;

[0103] Test mode: wet mode;

[0104] Dispersion medium: Use purified water as the dispersion medium;

[0105] Refractive index settings: The refractive index of the oil phase is set to 1.470, and the refractive index of water is set to 1.330;

[0106] Test temperature: maintained at room temperature of about 25°C;

[0107] Sampling volume: Approximately 1.5 mL of sample is used for each test.

[0108] 4. Anti-aging experiment:

[0109] Experimental animals: 40 female, 10-week-old, SPF-grade BALB / c mice (20 ± 1.5 g), purchased from Shanghai Laboratory Animal Center, production license number SCXK (Shanghai) 2020-0002, fed with standard feed, use license number SYXK (Shanghai) 2020-0003

[0110] 40 mice were randomly divided into 4 groups, 10 mice in each group, and kept in separate cages according to the groups. They were kept in a clean laboratory with good ventilation, temperature controlled at 22±2℃, humidity maintained at 50%-60%, and free access to food and water.

[0111] Experimental groups:

[0112] Blank control group: no treatment.

[0113] Model group: only received ultraviolet radiation to induce skin photoaging.

[0114] Experimental group: ultraviolet irradiation + external application of the anti-aging skin care composition of the present invention.

[0115] Ultraviolet irradiation conditions: Light source: UV-B lamp, wavelength 290-320nm.

[0116] Irradiation dose: 60mJ / cm 2 , once a day.

[0117] Irradiation site: mouse back skin, which had been depilated in advance.

[0118] Irradiation time: 6 weeks of continuous irradiation.

[0119] Topical products for treatment:

[0120] 30 minutes before UV irradiation, apply the experimental product evenly on the back skin of the mouse, with an application amount of 2 mg / cm 2 .

[0121] End of experiment and sampling:

[0122] When: After the 6th week.

[0123] Method of killing: cervical dislocation, in compliance with animal ethics requirements.

[0124] Tissue sampling:

[0125] A portion of dorsal skin tissue (approximately 2 cm×2 cm in size) was fixed in 10% neutral formalin solution for histological analysis.

[0126] The other part was quickly frozen in liquid nitrogen and then transferred to a -80°C freezer for biochemical index determination.

[0127] Detection indicators:

[0128] Biochemical analysis:

[0129] Antioxidant enzyme activity: Superoxide dismutase (SOD) activity was measured.

[0130] Lipid peroxidation product: malondialdehyde (MDA) content determination.

[0131] Histological analysis:

[0132] HE staining: observe the changes in skin tissue structure.

[0133] Table 1 Free radical scavenging rate

[0134] Grouping DPPH free radical scavenging rate (%) ABTS free radical scavenging rate (%) Example 1 92.3 93.7 Example 2 89.6 87.1 Example 3 87.5 85.4 Example 4 85.2 83.3 Example 5 79.8 77.9 Example 6 84.1 81.2 Comparative Example 1 75.3 73.0 Comparative Example 2 72.4 70.5

[0135] Example 1 demonstrates the best free radical scavenging effect, in which a specific ratio of ingredients (including resveratrol and sophora flavescens) provides the highest antioxidant activity. This result emphasizes the importance of ingredient ratios for enhancing product performance. Comparison of changes in ingredient concentrations in different embodiments and their effects on free radical scavenging rates shows that the synergistic effect between ingredients is crucial to improving the antioxidant effect of the product. In particular, the appropriate ratio of resveratrol and sophora flavescens significantly improves antioxidant performance. The data from the comparative examples show that increasing the concentration of resveratrol or sophora flavescens alone does not provide the same antioxidant effect as the composite ingredients, which confirms the necessity of a combination of multiple ingredients in the formula.

[0136] Table 2 Stability test data

[0137] sample D10(μm) D50(μm) D90(μm) Average particle size (μm) Example 1 0.85 4.25 9.60 4.90 Example 7 0.95 4.90 10.05 5.30 Example 8 0.88 4.60 9.80 5.10 Comparative Example 3 1.10 5.40 11.25 5.85 Comparative Example 4 1.15 5.60 11.55 6.05 Comparative Example 5 1.20 5.80 11.90 6.30

[0138] The emulsifier combination used in Example 1 (lecithin and polysorbate 80 in a ratio of 1:1.5) performed best in the stability test, showing excellent emulsification effect and particle size control. This result highlights the importance of carefully selecting and optimizing the ratio of emulsifiers. The emulsifier combination effectively reduces the emulsion particle size and improves the uniformity and stability of the emulsion, which is crucial for enhancing the skin penetration ability of active ingredients and maintaining the long-term stability of the formula. Compared with the use of a single emulsifier (such as Comparative Examples 3 and 4) or a non-specific target emulsifier (such as PEG-100 stearate used in Comparative Example 5), the emulsifier combination in Example 1 not only provides better stability, but may also have a positive impact on the effective delivery of active ingredients. The data show that when the emulsifier ratio is inappropriate (Examples 7 and 8), the particle size and stability increase, but are still better than the use of a single emulsifier.

[0139] Table 3 Anti-aging experimental data

[0140] Group SOD (U / mg prot) MDA (nmol / mg prot) Blank control group 117.34±6.37 2.6.±0.65 Model Group <![CDATA[72.48±5.89 A ]]> <![CDATA[7.85±0.78 A ]]> Example 1 <![CDATA[110.56±6.21 a ]]> 3.15±0.70 Example 2 105.33±6.47 <![CDATA[3.60±0.72 a ]]> Example 3 <![CDATA[102.89±5.95 a ]]> <![CDATA[3.85±0.68 a ]]> Example 4 <![CDATA[98.76±6.08 a ]]> 4.10±0.70 Example 5 92.45±6.52 <![CDATA[4.65±0.74 A ]]> Example 6 <![CDATA[96.11±6.23 a ]]> <![CDATA[4.40±0.69 a ]]> Comparative Example 1 <![CDATA[85.32±6.70 A ]]> 5.90±0.77 Comparative Example 2 <![CDATA[80.75±6.85 A ]]> <![CDATA[6.20±0.80 A ]]>

[0141] Note: Compared with the normal group, a p<0.05, A p<0.01

[0142] The test results fully demonstrate the beneficial effects of the anti-aging skin care composition of the present invention. Example 1 shows the best antioxidant performance, the SOD activity is significantly improved, and the MDA content is significantly reduced, which verifies that by using a specific emulsifier and accurately controlling the pH value, the stability of resveratrol and sophora japonica glycosides and their absorption rate in the skin can be improved, thereby enhancing the anti-aging effect. Although the effects of other embodiments (Examples 2 to 6) are slightly lower than those of Example 1, they are still significantly better than the model group and the comparative example group, reflecting the synergistic effect of resveratrol and sophora japonica glycosides after scientific proportioning, effectively improving the antioxidant efficacy and delaying skin aging. In contrast, due to the lack of core ingredients or improper proportions, Comparative Examples 1 and 2 have low SOD activity and high MDA content, which are close to the level of the model group. This further proves the importance of the combination of core ingredients and the key role of formula optimization in exerting anti-aging effects.

[0143] Histological analysis (HE staining) Figure 1-Figure 5 .

[0144] Blank control group: The mouse skin tissue structure was intact, the epidermis was flat and smooth, the boundary between the epidermis and dermis was clear, the epidermis was of normal thickness, the collagen fibers in the dermis were closely arranged, and the sweat glands and hair follicles were abundant and intact in structure.

[0145] Model group: After ultraviolet irradiation, the skin epidermis showed obvious wrinkling and irregular thickening, the thickness of the epidermis increased by about 30%, but the structure was disordered. The collagen fibers in the dermis were broken and arranged loosely, the thickness decreased by about 25%, the number of sweat glands and hair follicles decreased significantly, and the structure was damaged.

[0146] Example 1 group: Compared with the model group, the skin tissue structure was significantly improved. The flatness of the epidermis was restored, the thickness was close to the normal level, and the thickening degree was only half of that of the model group. The collagen fibers in the dermis were arranged more closely, the thickness increased by about 20%, the number of sweat glands and hair follicles increased significantly, and the structure tended to be complete.

[0147] Comparative Example 1: The skin tissue was improved, but the effect was not as good as that of Example 1. The thickness of the epidermis was slightly restored, but there was still irregular thickening, and the arrangement of collagen fibers in the dermis was improved, but the thickness did not increase significantly.

[0148] Comparative Example 2: The improvement effect is lower than that of the embodiment. The structure of the epidermis and dermis has recovered to a certain extent, but the thickness and fiber arrangement have not yet reached the ideal state.

[0149] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A collagen-enhanced skin care composition for anti-aging, characterized in that: The composition includes core active ingredients and auxiliary ingredients: The core ingredients include resveratrol and sophora flavescens, The auxiliary ingredients include active collagen, hyaluronic acid, trehalose, vitamin C or its derivatives, phenoxyethanol, carbomer, emulsifier, glycerin, and the balance is pure water.

2. The composition according to claim 1, characterized in that The concentration of resveratrol is 100-500 μg / mL, and the concentration of sophora flavescens is 100-500 μg / mL.

3. The composition according to claim 1, characterized in that The concentration of resveratrol was 200 μg / mL, and the concentration of sophora flavescens was 300 μg / mL.

4. The composition according to claim 1, characterized in that The auxiliary ingredients include 1-3% active collagen, 0.05-0.2% hyaluronic acid, 0.5-3% trehalose, 0.1-1% vitamin C or its derivatives, 0.5% phenoxyethanol, 0.1-0.5% carbomer, 0.03-0.35% triethanolamine, 1-3% emulsifier, 3-5% glycerol, and the balance is pure water.

5. The composition according to claim 1, characterized in that The emulsifier is a combination of lecithin and polysorbate.

6. The composition according to claim 2, characterized in that The mass ratio of the lecithin to the polysorbate is 1:1-1:

2.

7. The composition according to claim 1, characterized in that The vitamin C derivative is selected from one of ascorbyl tetraisopalmitate, ascorbyl glucoside, magnesium ascorbyl phosphate and sodium ascorbyl phosphate.

8. A method for preparing an anti-aging collagen-enhanced skin care composition, characterized in that: The following steps are involved: S1 Add 0.1-0.5% carbomer to pure water, stir until completely mixed, and then add 0.03-0.35% triethanolamine until the carbomer is completely dissolved and forms a uniform colloidal liquid; S2 adds 0.5-3% trehalose to the colloidal liquid of S1 in sequence, stirs until completely dissolved, then slowly adds 0.05-0.2% hyaluronic acid, and continues to stir until uniform, to prepare an aqueous phase; S3: 1-3% lecithin and polysorbate are mixed with 0.5% phenoxyethanol and 3-5% glycerol in a mass ratio of 1:1 to 1:2, and stirred and heated to 40° C. until a uniform oil phase is formed; S4: slowly add the oil phase into the water phase in portions, stirring at 40-50°C until a uniform emulsion is formed; S5: lower the temperature to room temperature, add 1-3% active collagen, 0.1-1% vitamin C or its derivatives in sequence, continue stirring until all active ingredients are evenly dispersed, and adjust the pH value to 5.0-7.0 to obtain auxiliary ingredients; S6, under light-proof conditions, adding resveratrol and sophora flavescens to the auxiliary ingredients, wherein the concentration of resveratrol is 50-600 μg / mL, and the concentration of sophora flavescens is 50-600 μg / mL, and stirring until uniformly dispersed; S7 ensures the uniformity and stability of the emulsion through vacuum degassing and high-speed homogenization, and finally encapsulates under sterile conditions.

9. Use of a composition in the preparation of anti-aging cosmetics, characterized in that: The composition is applied in emulsion, cream or facial mask.

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