Acne-removing composition based on pichia pastoris fermentation lysate filtrate

Abstract

The invention discloses an acne-removing composition based on pichia pastoris fermentation lysate filtrate, which is prepared from the following raw materials in parts by weight: deionized water, pichia pastoris fermentation lysate filtrate, wild chrysanthemum flower, saccharomycetes fermentation lysate filtrate, a preservative, hyaluronic acid, allantoin, hydrolyzed sclerotium gum, nicotinamide and erythritol. The invention relates to the technical field of skin repair. According to the acne-removing composition based on the pichia pastoris fermentation lysate filtrate, through the arrangement of the pichia pastoris fermentation lysate filtrate and the saccharomycetes fermentation lysate filtrate, the skin repairing capacity is improved, cells are activated, acnes are mildly removed, the hyaluronic acid is matched, the good moisturizing effect is achieved, the acnes are continuously and stably removed, and the acne-removing effect is good. The skin is effectively moistened and tightened, the cell metabolism is promoted, and the cell aging is prevented.

Description

Technical Field

[0001] The invention relates to the technical field of skin repair, in particular to an anti-acne composition based on a filtrate of a fermentation lysate of Pichia pastoris. Background Art

[0002] Acne is the common name for acne. "Acne" is the same as acne. It is also called "facial pimples", "blackheads", "wine pimples", "dark pimples", etc. It is a chronic inflammatory skin disease caused by excessive sebum secretion, blockage of the sebaceous gland ducts of the hair follicles, bacterial infection and inflammatory response.

[0003] Acne seriously affects people's appearance. There are many types of acne removal products at present, but the quality is uneven. In addition, most of them have the problem of high content of chemical ingredients, strong irritation, and easy recurrence of treatment, which cannot achieve effective acne removal effect. Summary of the invention

[0004] 1. Technical issues to be resolved

[0005] In view of the deficiencies in the prior art, the present invention provides an anti-acne composition based on the filtrate of Pichia pastoris fermentation lysate, which solves the above-mentioned problems.

[0006] (II) Technical solution

[0007] To achieve the above objectives, the present invention is implemented through the following technical scheme: an anti-acne composition based on Pichia pastoris fermentation lysate filtrate, wherein the raw materials include by weight: 84-96 parts of deionized water, 3-10 parts of Pichia pastoris fermentation lysate filtrate, 3-5 parts of wild chrysanthemum, 0.1-5 parts of yeast fermentation lysate filtrate, 0.1-1 parts of preservatives, 1-3 parts of hyaluronic acid, 0.1-0.5 parts of allantoin, 0.1-1 parts of hydrolyzed sclerotium gum, 0.1-5 parts of niacinamide, and 1-5 parts of erythritol.

[0008] The present invention is further configured as follows: the raw materials include, by weight: 96 parts of deionized water, 10 parts of Pichia pastoris fermentation lysate filtrate, 3 parts of wild chrysanthemum, 5 parts of yeast fermentation lysate filtrate, 1 part of preservative, 1 part of hyaluronic acid, 0.5 parts of allantoin, 1 part of hydrolyzed sclerotium gum, 5 parts of nicotinamide, and 5 parts of erythritol.

[0009] The present invention is further configured as follows: the raw materials include, by weight: 91 parts of deionized water, 7 parts of Pichia pastoris fermentation lysate filtrate, 4 parts of wild chrysanthemum, 2.8 parts of yeast fermentation lysate filtrate, 0.5 parts of preservative, 2 parts of hyaluronic acid, 0.3 parts of allantoin, 0.5 parts of hydrolyzed sclerotium gum, 3 parts of nicotinamide, and 3 parts of erythritol.

[0010] The present invention is further configured as follows: the raw materials include, by weight: 84 parts of deionized water, 3 parts of Pichia pastoris fermentation lysate filtrate, 5 parts of wild chrysanthemum, 0.1 part of yeast fermentation lysate filtrate, 0.1 part of preservative, 3 parts of hyaluronic acid, 0.1 part of allantoin, 0.1 part of hydrolyzed sclerotium gum, 0.1 part of niacinamide, and 1 part of erythritol.

[0011] The present invention is further configured as follows: the preservative comprises one or more of propolis, protamine and tea polyphenols.

[0012] The present invention is further configured as follows: the preparation method of the anti-acne composition based on the filtrate of the fermentation lysate of Pichia pastoris comprises the following steps:

[0013] Step 1, preparation of base liquid A: wash and dry wild chrysanthemum, grind into powder, pass through a 20-mesh sieve to obtain wild chrysanthemum powder, add to deionized water, stir evenly, add allantoin, hydrolyzed sclerotium gum, niacinamide and erythritol to obtain a prepared base liquid A;

[0014] Step 2, preparation of base solution B: adding the yeast fermentation lysate filtrate to the Pichia pastoris fermentation lysate filtrate, while maintaining stirring, adding hyaluronic acid, stirring evenly, and obtaining the prepared base solution B;

[0015] Step 3, preparation of semi-finished product: add the base liquid B dropwise into the base liquid A, keep stirring, add preservatives during the process, keep the temperature at 25°C for 15 minutes, and obtain the semi-finished product;

[0016] Step 4: Packaging of finished products: Keep stirring at room temperature for 1h-1.5h, pack and seal to obtain the finished products.

[0017] (III) Beneficial effects

[0018] The present invention provides an anti-acne composition based on the filtrate of Pichia pastoris fermentation lysate. It has the following beneficial effects:

[0019] The present invention improves the skin repair ability and activates cells through the setting of Pichia pastoris fermentation lysate filtrate and yeast fermentation lysate filtrate, thereby achieving the purpose of mild acne removal. In combination with the setting of hyaluronic acid, it has a good moisturizing effect and provides long-term stable acne removal, effectively moisturizes and tightens the skin, promotes cell metabolism and prevents cell aging. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic diagram of the process of the present invention. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0022] See also Figure 1 , the embodiment of the present invention provides the following technical solutions:

[0023] Embodiment 1

[0024] The invention discloses an acne-removing composition based on a filtrate of a fermentation lysate of Pichia pastoris. The raw materials thereof include, by weight, 96 parts of deionized water, 10 parts of a filtrate of a fermentation lysate of Pichia pastoris, 3 parts of wild chrysanthemum, 5 parts of a filtrate of a fermentation lysate of yeast, 1 part of a preservative, 1 part of hyaluronic acid, 0.5 parts of allantoin, 1 part of hydrolyzed sclerotium gum, 5 parts of niacinamide, and 5 parts of erythritol.

[0025] The preparation method of the anti-acne composition based on the filtrate of Pichia pastoris fermentation lysate comprises the following steps:

[0026] Step 1, preparation of base liquid A: wash and dry wild chrysanthemum, grind into powder, pass through a 20-mesh sieve to obtain wild chrysanthemum powder, and weigh deionized water, Pichia pastoris fermentation lysate filtrate, wild chrysanthemum powder, yeast fermentation lysate filtrate, preservative, hyaluronic acid, allantoin, hydrolyzed sclerotium gum, niacinamide, and erythritol according to a weight ratio of 96:10:10:3:5:8:1:1:3:0.5:1:5:5;

[0027] The weighed wild chrysanthemum powder was added into deionized water, and after being stirred evenly, allantoin, hydrolyzed sclerotium gum, niacinamide and erythritol were added to obtain a preparation base solution A;

[0028] Step 2, preparation of base solution B: adding the yeast fermentation lysate filtrate to the Pichia pastoris fermentation lysate filtrate, while maintaining stirring, adding hyaluronic acid, stirring evenly, and obtaining the prepared base solution B;

[0029] Step 3, preparation of semi-finished product: add the base liquid B dropwise into the base liquid A, keep stirring, add preservatives during the process, keep the temperature at 25°C for 15 minutes, and obtain the semi-finished product;

[0030] Step 4: Packaging of finished products: Keep stirring at room temperature for 1h-1.5h, pack and seal to obtain the finished products.

[0031] Embodiment 2

[0032] The invention discloses an acne-removing composition based on a filtrate of a fermentation lysate of Pichia pastoris. The raw materials thereof include, by weight, 91 parts of deionized water, 7 parts of a filtrate of a fermentation lysate of Pichia pastoris, 4 parts of wild chrysanthemum, 2.8 parts of a filtrate of a fermentation lysate of yeast, 0.5 parts of a preservative, 2 parts of hyaluronic acid, 0.3 parts of allantoin, 0.5 parts of hydrolyzed sclerotium gum, 3 parts of niacinamide, and 3 parts of erythritol.

[0033] The preparation method of the anti-acne composition based on the filtrate of Pichia pastoris fermentation lysate comprises the following steps:

[0034] Step 1, preparation of base liquid A: after washing and drying wild chrysanthemum, grind into powder, pass through a 20-mesh sieve to obtain wild chrysanthemum powder, and weigh deionized water, Pichia pastoris fermentation lysate filtrate, wild chrysanthemum powder, yeast fermentation lysate filtrate, preservative, hyaluronic acid, allantoin, hydrolyzed sclerotium gum, niacinamide, and erythritol according to a weight ratio of 91:12:7:4:2.8:6:0.5:2:4:0.3:0.5:3:3;

[0035] The weighed wild chrysanthemum powder was added into deionized water, and after being stirred evenly, allantoin, hydrolyzed sclerotium gum, niacinamide and erythritol were added to obtain a preparation base solution A;

[0036] Step 2, preparation of base solution B: adding the yeast fermentation lysate filtrate to the Pichia pastoris fermentation lysate filtrate, while maintaining stirring, adding hyaluronic acid, stirring evenly, and obtaining the prepared base solution B;

[0037] Step 3, preparation of semi-finished product: add the base liquid B dropwise into the base liquid A, keep stirring, add preservatives during the process, keep the temperature at 25°C for 15 minutes, and obtain the semi-finished product;

[0038] Step 4: Packaging of finished products: Keep stirring at room temperature for 1h-1.5h, pack and seal to obtain the finished products.

[0039] Embodiment 3

[0040] An acne-removing composition based on a filtrate of a fermentation lysate of Pichia pastoris comprises, by weight, 84 parts of deionized water, 3 parts of a filtrate of a fermentation lysate of Pichia pastoris, 5 parts of wild chrysanthemum, 0.1 part of a filtrate of a fermentation lysate of yeast, 0.1 part of a preservative, 3 parts of hyaluronic acid, 0.1 part of allantoin, 0.1 part of hydrolyzed sclerotium gum, 0.1 part of niacinamide, and 1 part of erythritol.

[0041] The preparation method of the anti-acne composition based on the filtrate of Pichia pastoris fermentation lysate comprises the following steps:

[0042] Step 1, preparation of base liquid A: after washing and drying wild chrysanthemum, grind into powder, pass through a 20-mesh sieve to obtain wild chrysanthemum powder, and weigh deionized water, Pichia pastoris fermentation lysate filtrate, wild chrysanthemum powder, yeast fermentation lysate filtrate, preservative, hyaluronic acid, allantoin, hydrolyzed sclerotium gum, niacinamide, and erythritol according to a weight ratio of 84:18:3:5:0.1:3:0.1:3:5:0.1:0.1:0.1:1;

[0043] The weighed wild chrysanthemum powder was added into deionized water, and after being stirred evenly, allantoin, hydrolyzed sclerotium gum, niacinamide and erythritol were added to obtain a preparation base solution A;

[0044] Step 2, preparation of base solution B: adding the yeast fermentation lysate filtrate to the Pichia pastoris fermentation lysate filtrate, while maintaining stirring, adding hyaluronic acid, stirring evenly, and obtaining the prepared base solution B;

[0045] Step 3, preparation of semi-finished product: add the base liquid B dropwise into the base liquid A, keep stirring, add preservatives during the process, keep the temperature at 25°C for 15 minutes, and obtain the semi-finished product;

[0046] Step 4: Packaging of finished products: Keep stirring at room temperature for 1h-1.5h, pack and seal to obtain the finished products.

[0047] After the finished products were prepared by using the above-mentioned Example 1, Example 2 and Example 3, three portions of the finished products were taken as test samples respectively.

[0048] Multiple skin irritation tests:

[0049] Take four New Zealand white rabbits, cut the hair on both sides of the rabbit's spine, take the test sample and apply it on one side of the skin, with an area of ​​2.5CM*2.5CM as the test area, and leave the other side untreated as the control area. Apply once a day for 14 consecutive days. Starting from the second day, cut the hair before each application, and use pure water to remove the residual test sample. Observe the test results after one hour, compare the test area with the control area, and find that the test sample is not irritating.

[0050] Acute skin irritation test:

[0051] Four New Zealand white rabbits were taken, and after cutting off the hair on both sides of the rabbit's spine on the back, the test sample was smeared on one side of the skin, with an area of ​​2.5CM*2.5CM. After covering it with two layers of gauze and a layer of cellophane, it was fixed with non-irritating tape and a bandage as the test area, and the other side was left untreated as the control area. After 4 hours, the residual test sample was removed with pure water, and the test area was compared with the control area. It was found that the test sample was not irritating.

[0052] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions. The sentence "includes an element defined by ... does not exclude the existence of other identical elements in the process, method, article or device including the element".

[0053] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An anti-acne composition based on a filtrate of a fermentation lysate of Pichia pastoris, characterized in that: The raw materials include, by weight, 84-96 parts of deionized water, 3-10 parts of Pichia pastoris fermentation lysate filtrate, 3-5 parts of wild chrysanthemum, 0.1-5 parts of yeast fermentation lysate filtrate, 0.1-1 parts of preservatives, 1-3 parts of hyaluronic acid, 0.1-0.5 parts of allantoin, 0.1-1 parts of hydrolyzed sclerotium gum, 0.1-5 parts of nicotinamide and 1-5 parts of erythritol.

2. The acne-removing composition based on the filtrate of Pichia pastoris fermentation lysate according to claim 1, characterized in that: The raw materials include, by weight, 96 parts of deionized water, 10 parts of Pichia pastoris fermentation lysate filtrate, 3 parts of wild chrysanthemum, 5 parts of yeast fermentation lysate filtrate, 1 part of preservative, 1 part of hyaluronic acid, 0.5 parts of allantoin, 1 part of hydrolyzed sclerotium gum, 5 parts of nicotinamide and 5 parts of erythritol.

3. The acne-removing composition based on the filtrate of Pichia pastoris fermentation lysate according to claim 1, characterized in that: The raw materials include, by weight, 91 parts of deionized water, 7 parts of Pichia pastoris fermentation lysate filtrate, 4 parts of wild chrysanthemum, 2.8 parts of yeast fermentation lysate filtrate, 0.5 parts of preservative, 2 parts of hyaluronic acid, 0.3 parts of allantoin, 0.5 parts of hydrolyzed sclerotium gum, 3 parts of nicotinamide and 3 parts of erythritol.

4. The anti-acne composition based on the filtrate of Pichia pastoris fermentation lysate according to claim 1, characterized in that: The raw materials include, by weight: 84 parts of deionized water, 3 parts of Pichia pastoris fermentation lysate filtrate, 5 parts of wild chrysanthemum, 0.1 parts of yeast fermentation lysate filtrate, 0.1 parts of preservatives, 3 parts of hyaluronic acid, 0.1 parts of allantoin, 0.1 parts of hydrolyzed sclerotium gum, 0.1 parts of niacinamide and 1 part of erythritol.

5. An anti-acne composition based on the filtrate of Pichia pastoris fermentation lysate according to any one of claims 1 to 4, characterized in that: The preservative comprises one or more of propolis, protamine and tea polyphenols.

6. An anti-acne composition based on the filtrate of Pichia pastoris fermentation lysate according to any one of claims 1 to 4, characterized in that: The preparation method of the anti-acne composition based on the filtrate of Pichia pastoris fermentation lysate comprises the following steps: Step 1, preparation of base liquid A: wash and dry wild chrysanthemum, grind into powder, pass through a 20-mesh sieve to obtain wild chrysanthemum powder, add to deionized water, stir evenly, add allantoin, hydrolyzed sclerotium gum, niacinamide and erythritol to obtain a prepared base liquid A; Step 2, preparation of base solution B: adding the yeast fermentation lysate filtrate to the Pichia pastoris fermentation lysate filtrate, while maintaining stirring, adding hyaluronic acid, stirring evenly, and obtaining the prepared base solution B; Step 3, preparation of semi-finished product: add the base liquid B dropwise into the base liquid A, keep stirring, add preservatives during the process, keep the temperature at 25°C, keep for 15 minutes, and obtain the semi-finished product; Step 4: Finished product packaging: Stir for 1h-1.5h, package and seal to obtain the finished product.

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