Fermentation product for relieving spleen deficiency and dampness obstruction type simple obesity and preparation method thereof

Abstract

The invention belongs to the technical field of traditional Chinese medicine fermentation, and particularly relates to a fermented product capable of relieving spleen deficiency and dampness obstruction type simple obesity. The fermentation composition comprises the following components in parts by weight: 80-150 parts of exocarpium citri grandis, 15-40 parts of pericarpium citri reticulatae, 5-30 parts of radix fici simplicissimae, 0.1-0.22 part of a leavening agent and 8-18 parts of an enzyme preparation. The traditional Chinese medicine fermented composition is provided for relieving spleen deficiency and dampness obstruction type obesity, and has good effects of tonifying spleen, clearing damp and losing weight.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicine fermentation, and particularly relates to a fermentation composition capable of alleviating simple obesity of spleen deficiency and dampness resistance type and a preparation method thereof. Background Art

[0002] Simple obesity refers to a disease in which the body's energy intake is greater than its energy consumption, excluding other nervous or endocrine system diseases, resulting in excessive accumulation of subcutaneous and visceral fat. From the perspective of TCM syndromes, simple obesity is divided into multiple syndromes, including spleen deficiency and dampness obstruction, yin deficiency and internal heat, liver stasis and qi stagnation, etc. Among them, spleen deficiency and dampness obstruction simple obesity is the most common in clinical practice. It is mainly due to the fact that patients overeat fatty and sweet foods in their daily lives, which damages the spleen function. In addition, the influence of factors such as lack of exercise, long-term sitting and lying, leads to unfavorable rise and fall of qi, and a large amount of phlegm and dampness accumulate in the body, eventually causing obesity.

[0003] Simple obesity in Western medicine refers to secondary obesity that is independent of other diseases or medical reasons, and is caused only by energy intake exceeding consumption; obesity is usually referred to as simple obesity. At present, Western medicine mainly treats simple obesity with drugs and surgery, but long-term medication can easily cause adverse reactions in multiple systems of the body, and it is easy to relapse after stopping the medication. Surgical treatment has limitations such as strict indications and high prices, making it difficult to achieve significant therapeutic effects.

[0004] In recent years, the use of traditional Chinese medicine concepts and treatment plans has been favored by patients with simple obesity of spleen deficiency and dampness obstruction type. Chinese medicine for strengthening the spleen and removing dampness can strengthen the spleen and replenish qi, remove dampness and promote stagnation, and thus achieve the effect of weight loss. For example, Chinese patent CN110652570A discloses a Chinese medicine composition for strengthening the spleen and removing dampness, and the raw materials of the Chinese medicine composition for strengthening the spleen and removing dampness include Amomum villosum, Pueraria root, Chinese yam, Poria cocos, Hericium erinaceus, orange peel, red dates, licorice, lotus leaf, five-fingered peach, burdock, chicory, jujube, shiitake mushroom, white pepper and mulberry leaf. For production factories, the composition involves too many Chinese medicines, the cost is high, and the Chinese medicine decoction process is more complicated and not suitable for large-scale production. And the patent effect is mainly to strengthen the spleen, eliminate dampness and nourish the stomach, and does not involve weight loss effect. Summary of the invention

[0005] To this end, the present invention provides a fermented composition for simple obesity of spleen deficiency and dampness resistance type. The composition comprises three raw materials, namely, tangerine peel, tangerine peel, and is prepared after being fermented by lactic acid bacteria. The composition can be used to treat simple obesity of spleen deficiency and dampness resistance type.

[0006] To achieve the above object, the scheme adopted by the present invention is as follows:

[0007] On the one hand, the present invention provides a fermented product for alleviating simple obesity of spleen deficiency and dampness obstruction type. The fermented raw materials include the following components by weight: 80-150 parts of tangerine peel, 15-40 parts of tangerine peel, 5-30 parts of quinquefolius, 0.1-0.22 parts of starter and 8-18 parts of enzyme preparation.

[0008] Preferably, the fermentation raw materials include the following components in parts by weight: 90-130 parts of dried tangerine peel, 20-30 parts of dried tangerine peel, 10-20 parts of quinquefolius, 0.12-0.18 parts of starter and 10-15 parts of enzyme preparation.

[0009] Further preferably, the fermentation raw materials include the following components in parts by weight: 110 parts of dried tangerine peel, 25 parts of tangerine peel, 15 parts of quinquefolius, 0.15 parts of starter, and 12 parts of enzyme preparation.

[0010] Preferably, the fermentation agent includes Lactobacillus reuteri and Bifidobacterium longum subsp. longum; and the enzyme preparation includes cellulase and pectinase.

[0011] Preferably, the fermentation agent comprises 0.07-0.16 parts of Lactobacillus reuteri and 0.03-0.06 parts of Bifidobacterium longum subsp. longum.

[0012] Further preferably, the weight ratio of Lactobacillus reuteri to Bifidobacterium longum subsp. longum in the starter is 2:1.

[0013] More preferably, the viable bacterial count of Lactobacillus reuteri is 10-400 billion CFU / g, and the viable bacterial count of Bifidobacterium longum subspecies longum is 10-400 billion CFU / g.

[0014] Further preferably, the enzyme preparation comprises 5-11 parts of cellulase and 3-7 parts of pectinase.

[0015] More preferably, the weight ratio of the cellulase to the pectinase is 2:1, wherein the enzymatic activity of the cellulase is 20,000-40,000 U / g, and the enzymatic activity of the pectinase is 30,000-50,000 U / g.

[0016] On the other hand, the present invention also provides a method for preparing a fermented composition for alleviating simple obesity of spleen deficiency and dampness obstruction type, comprising the following steps:

[0017] (1) mixing tangerine peel, dried orange peel, and citrus aurantium with water to obtain a mixed solution;

[0018] (2) adding an enzyme preparation to the mixed solution for enzymolysis, followed by microwave treatment, and adjusting the pH to 6-7 to obtain an enzymolysis solution;

[0019] (3) raising the temperature of the enzymatic hydrolysis solution to perform enzyme inactivation extraction treatment to obtain an enzyme inactivation extract solution;

[0020] (4) when the temperature of the enzyme-killed extract drops to 30-40° C., adding a fermentation agent for fermentation to obtain a fermentation liquid;

[0021] (5) The fermentation liquid is centrifuged, and the supernatant is the fermentation liquid.

[0022] Preferably, in step (2), the enzymatic hydrolysis temperature is 50-60° C., the enzymatic hydrolysis time is 1-3 h, the microwave time is 3-12 min, and the microwave power is 500-900 W.

[0023] Preferably, the enzyme inactivation extraction temperature in step (3) is 90-115° C., and the enzyme inactivation extraction time is 40-90 min.

[0024] Preferably, the fermentation temperature of step (4) is 35-42° C. and the fermentation time is 10-50 h.

[0025] Preferably, the method further comprises the step of crushing the dried orange peel, dried tangerine peel and quinquefolius into powder.

[0026] More preferably, the method for preparing the fermentation composition further comprises the step of concentrating the fermentation supernatant to obtain a fermentation paste.

[0027] In another aspect, the present invention provides a fermentation preparation, which comprises: the above-mentioned fermentation composition and auxiliary materials.

[0028] The auxiliary material is at least one of maltodextrin, mannose, skim milk powder, cyclodextrin, glycerol and starch.

[0029] Finally, the present invention provides an application of the above-mentioned fermentation composition, the fermentation composition prepared by the above-mentioned method, or the above-mentioned fermentation preparation in the preparation of medicines, health foods, foods, etc. for treating simple obesity of spleen deficiency and dampness obstruction type.

[0030] The technical solution of the present invention has the following advantages:

[0031] 1. The fermented composition provided by the present invention has simple raw materials. The selected five-fingered ginseng, tangerine peel and dried tangerine peel have a history of being used as both medicine and food. The five-fingered ginseng is flat in nature, sweet and slightly warm in taste, and has the effects of invigorating qi and strengthening the spleen, removing phlegm and eliminating dampness, and relaxing muscles and activating collaterals; dried tangerine peel is warm in nature, bitter and pungent in taste, and enters the lung and spleen meridians, and has the effects of regulating qi and strengthening the spleen, and drying dampness and eliminating phlegm; dried tangerine peel is warm in nature, pungent, bitter and aromatic in taste, and enters the lung and spleen meridians. Pungent ones are good at dispersing, can circulate qi and blood, and help the transportation and transformation of body fluids; bitter taste can relieve fire, dry dampness, and strengthen yin, and is good at clearing heat and drying dampness. Therefore, dried tangerine peel is good at regulating qi and relieving fullness, and pungent ones are good at dispersing dampness and eliminating phlegm. The three raw materials cooperate synergistically to give full play to their respective effects in the composition, and the obtained composition can be used for the treatment of simple obesity of spleen deficiency and dampness obstruction type.

[0032] 2. The traditional method of decocting Chinese medicine is complicated and time-consuming. The preparation method of the fermentation composition provided by the present invention, through the process of enzymatic hydrolysis + microwave + fermentation, firstly promotes the effective infiltration of Chinese medicine components under the action of composite enzymes, and then further promotes the dissolution of Chinese medicine components through microwaves. Finally, through fermentation, large molecules that are difficult to absorb can be further converted into small molecules that are easily absorbed, thereby improving the efficacy of Chinese medicine.

[0033] 3. The fermentation effect of the composite strain of Lactobacillus reuteri and Bifidobacterium longum subspecies longum used in the present invention is better than that of single strain fermentation, which improves the fermentation efficiency and enhances the spleen-strengthening, dampness-removing and weight-reducing effects of the fermented product. DETAILED DESCRIPTION

[0034] The following non-limiting examples can enable those skilled in the art to more fully understand the present invention, but do not limit the present invention in any way. The following content is merely an exemplary description of the scope of the present invention, and those skilled in the art can make various changes and modifications to the present invention based on the disclosed content, which should also fall within the scope of the present invention.

[0035] When the embodiments give numerical ranges, it should be understood that, unless otherwise specified in the present invention, both endpoints of each numerical range and any numerical value between the two endpoints can be selected. Unless otherwise defined, all technical and scientific terms used in the present invention have the same meanings as those generally understood by those of ordinary skill in the art to which the present invention belongs.

[0036] The present invention is further described below by way of specific examples. The various chemical reagents used in the examples of the present invention are obtained through conventional commercial channels unless otherwise specified. Products from different manufacturers have no significant effect on the effects.

[0037] In the following examples, the source information of some products is as follows:

[0038] Lactobacillus reuteri and Bifidobacterium longum subsp. longum were purchased from Shandong Zhongke Jiayi Bioengineering Co., Ltd. Lactobacillus reuteri JYLB-131 and Bifidobacterium longum subsp. longum JYLC-141; cellulase and pectinase were purchased from Shandong Longkote Enzyme Preparation Co., Ltd.

[0039] Example 1

[0040] The present embodiment provides a fermented product for alleviating simple obesity of spleen deficiency and dampness obstruction type, comprising 110g of dried tangerine peel, 25g of dried tangerine peel, 15g of five-fingered peach, 8g of cellulase, 4g of pectinase, 0.1g of Lactobacillus reuteri, 0.05g of Bifidobacterium longum subsp. longum and 1500mL of water; wherein the enzymatic activity of cellulase is 20000U / g, the enzymatic activity of pectinase is 40000U / g, the viable bacterial count of Lactobacillus reuteri is 100 billion CFU / g, and the viable bacterial count of Bifidobacterium longum subsp. longum is 100 billion CFU / g.

[0041] Its preparation method is:

[0042] (1) crushing the dried orange peel, dried tangerine peel and five-fingered ginseng into powder respectively, taking 110g of dried orange peel powder, 25g of dried tangerine peel powder and 15g of five-fingered ginseng powder, adding 1500mL of water to mix, and using sodium bicarbonate to adjust the pH to 4.5 to obtain a mixed solution;

[0043] (2) adding 8 g of cellulase and 4 g of pectinase to the mixed solution, performing enzymolysis at 55° C. for 2 h, and then subjecting the mixture to microwave treatment at a power of 600 W for 10 min. Using sodium bicarbonate to adjust the pH to 6.8, an enzymolysis treatment solution was obtained;

[0044] (3) raising the temperature of the enzymatic hydrolysis solution to 100° C. and performing enzyme inactivation extraction for 50 minutes to obtain an enzyme inactivation extract;

[0045] (4) When the temperature of the enzyme-killing extract drops to 40° C., add 0.1 g of Lactobacillus reuteri and 0.05 g of Bifidobacterium longum subsp. longum, and ferment at this temperature for 45 h to obtain a fermentation liquid;

[0046] (5) The fermentation liquid was centrifuged to obtain the supernatant, which was the fermentation liquid. The pH value of the fermentation liquid was 3.7, the soluble solid content was 13.2%, and the total acid content as lactic acid was 2.13%.

[0047] Example 2

[0048] The present embodiment provides a fermented product for alleviating simple obesity of spleen deficiency and dampness obstruction type, comprising 150g of dried tangerine peel, 15g of dried tangerine peel, 10g of quinquefolius, 11g of cellulase, 3g of pectinase, 0.16g of Lactobacillus reuteri, 0.03g of Bifidobacterium longum subsp. longum and 1750mL of water; wherein the enzymatic activity of the cellulase is 20000U / g, the enzymatic activity of the pectinase is 40000U / g, the viable bacterial count of Lactobacillus reuteri is 100 billion CFU / g, and the viable bacterial count of Bifidobacterium longum subsp. longum is 100 billion CFU / g.

[0049] Its preparation method is:

[0050] (1) crushing tangerine peel, dried orange peel and quinquefolius chinensis into powder respectively, taking tangerine peel powder 150g, dried orange peel powder 15g, quinquefolius chinensis powder 10g, adding 1750mL water to mix, using sodium bicarbonate to adjust the pH to 4.8, to obtain a mixed solution;

[0051] (2) adding 11 g of cellulase and 3 g of pectinase to the mixed solution, performing enzymolysis at 60° C. for 1 h, and then subjecting the mixture to microwave treatment at 900 W for 3 min. Using sodium bicarbonate to adjust the pH to 6.5, an enzymolysis treatment solution was obtained;

[0052] (3) raising the temperature of the enzymatic hydrolysis solution to 115° C. and performing enzyme inactivation extraction for 40 minutes to obtain an enzyme inactivation extract;

[0053] (4) When the temperature of the enzyme-killing extract drops to 42° C., add 0.16 g of Lactobacillus reuteri and 0.03 g of Bifidobacterium longum subsp. longum, and ferment at this temperature for 10 h to obtain a fermentation liquid;

[0054] (5) The fermentation liquid was centrifuged to obtain a supernatant, the pH of the fermentation liquid was 3.62, the soluble solid content was 12.2%, and the total acid content as lactic acid was 2.16%;

[0055] (6) The fermentation liquid was concentrated by vacuum low-temperature concentration process to obtain fermentation paste, the concentration temperature was 65°C, the vacuum degree was -0.09MPa, and the fermentation paste density was 1.26g / cm 3 , soluble solids content 34.6%;

[0056] (7) Add 20 parts by weight of skimmed milk powder to the fermentation paste, and dry it by spray drying to obtain a fermentation preparation; wherein the spray drying process is as follows: air inlet temperature 128°C, air outlet temperature 75°C, pump speed 18%, and the moisture content of the fermentation preparation is 8.3%.

[0057] Example 3

[0058] The present embodiment provides a fermented product for alleviating simple obesity of spleen deficiency and dampness obstruction type, comprising 80g of dried tangerine peel, 40g of dried tangerine peel, 5g of quinquefolius, 5g of cellulase, 7g of pectinase, 0.07g of Lactobacillus reuteri, 0.06g of Bifidobacterium longum subsp. longum and 1250mL of water; wherein the enzymatic activity of the cellulase is 20000U / g, the enzymatic activity of the pectinase is 40000U / g, the viable bacterial count of Lactobacillus reuteri is 100 billion CFU / g, and the viable bacterial count of Bifidobacterium longum subsp. longum is 100 billion CFU / g.

[0059] Its preparation method is:

[0060] (1) crushing tangerine peel, dried orange peel and quinquefolius chinensis into powder respectively, taking tangerine peel powder 80g, dried orange peel powder 40g, quinquefolius chinensis powder 5g, adding 1250mL water to mix, using sodium bicarbonate to adjust the pH to 4, to obtain a mixed solution;

[0061] (2) adding 5 g of cellulase and 7 g of pectinase to the mixed solution, performing enzymolysis at 50° C. for 3 h, and then subjecting the mixture to microwave treatment at 500 W for 12 min, adjusting the pH to 6 with sodium bicarbonate to obtain an enzymolysis solution;

[0062] (3) raising the temperature of the enzymatic hydrolysis solution to 90° C. and performing enzyme inactivation extraction for 90 minutes to obtain an enzyme inactivation extract;

[0063] (4) When the temperature of the enzyme-killing extract drops to 35° C., add 0.07 g of Lactobacillus reuteri and 0.06 g of Bifidobacterium longum subsp. longum, and ferment at this temperature for 50 h to obtain a fermentation liquid;

[0064] (5) The fermentation liquid was centrifuged to obtain the supernatant, which was the fermentation liquid. The pH of the fermentation liquid was 3.66, the soluble solid content was 13.4%, and the total acid content, calculated as lactic acid, was 2.21%.

[0065] Example 4

[0066] The present embodiment provides a fermented product for alleviating simple obesity of spleen deficiency and dampness obstruction type, comprising 130g of dried tangerine peel, 20g of dried tangerine peel, 30g of five-fingered peach, 8g of cellulase, 5g of pectinase, 0.12g of Lactobacillus reuteri, 0.04g of Bifidobacterium longum subsp. longum and 1800mL of water; wherein the enzymatic activity of the cellulase is 20000U / g, the enzymatic activity of the pectinase is 40000U / g, the viable bacterial count of Lactobacillus reuteri is 100 billion CFU / g, and the viable bacterial count of Bifidobacterium longum subsp. longum is 100 billion CFU / g.

[0067] Its preparation method is:

[0068] (1) crushing the red tangerine peel, dried tangerine peel and five-fingered ginseng into powder respectively, taking 130g of red tangerine peel powder, 20g of dried tangerine peel powder and 30g of five-fingered ginseng powder, adding 1800mL of water to mix, and using sodium bicarbonate to adjust the pH to 4.2 to obtain a mixed solution;

[0069] (2) adding 8 g of cellulase and 5 g of pectinase to the mixed solution, performing enzymolysis at 52° C. for 2.5 h, and then subjecting the mixture to microwave treatment at a power of 750 W for 6 min, adjusting the pH to 6.2 using sodium bicarbonate, and obtaining an enzymolysis treatment solution;

[0070] (3) raising the temperature of the enzymatic hydrolysis solution to 110° C. and performing enzyme inactivation extraction for 60 minutes to obtain an enzyme inactivation extract;

[0071] (4) When the temperature of the enzyme-killing extract drops to 38° C., add 0.12 g of Lactobacillus reuteri and 0.04 g of Bifidobacterium longum subsp. longum, and ferment at this temperature for 30 h to obtain a fermentation liquid;

[0072] (5) The fermentation liquid was centrifuged to obtain the supernatant, which was the fermentation liquid. The pH of the fermentation liquid was 3.62, the soluble solid content was 12.2%, and the total acid content (calculated as lactic acid) was 2.16%.

[0073] Example 5

[0074] The present embodiment provides a fermented product for alleviating simple obesity of spleen deficiency and dampness obstruction type, comprising 90g of dried tangerine peel, 30g of dried tangerine peel, 20g of five-fingered peach, 6g of cellulase, 6g of pectinase, 0.14g of Lactobacillus reuteri, 0.06g of Bifidobacterium longum subsp. longum and 1400mL of water; wherein the enzymatic activity of the cellulase is 20000U / g, the enzymatic activity of the pectinase is 40000U / g, the viable bacterial count of Lactobacillus reuteri is 100 billion CFU / g, and the viable bacterial count of Bifidobacterium longum subsp. longum is 100 billion CFU / g.

[0075] Its preparation method is:

[0076] (1) crushing tangerine peel, dried orange peel and quinquefolius chinensis into powder respectively, taking tangerine peel powder 90g, dried orange peel powder 30g, quinquefolius chinensis powder 20g, adding 1400mL water to mix, using sodium bicarbonate to adjust the pH to 4.8, to obtain a mixed solution;

[0077] (2) adding 6 g of cellulase and 6 g of pectinase to the mixed solution, performing enzymolysis at 58° C. for 1.5 h, and then subjecting the mixture to microwave treatment at 800 W for 9 min. Using sodium bicarbonate to adjust the pH to 6.4, an enzymolysis treatment solution was obtained;

[0078] (3) raising the temperature of the enzymatic hydrolysis solution to 102° C. and performing enzyme inactivation extraction for 70 minutes to obtain an enzyme inactivation extract;

[0079] (4) When the temperature of the enzyme-killing extract drops to 39° C., add 0.14 g of Lactobacillus reuteri and 0.06 g of Bifidobacterium longum subsp. longum, and ferment at this temperature for 20 h to obtain a fermentation liquid;

[0080] (5) The fermentation liquid was centrifuged to obtain the supernatant, which was the fermentation liquid. The pH of the fermentation liquid was 3.55, the soluble solid content was 11.9%, and the total acid content, calculated as lactic acid, was 2.14%.

[0081] Comparative Example 1

[0082] This comparative example provides a fermented product, and the difference between this comparative example and Example 1 is that the proportion of Huajuhong is smaller, Huajuhong 50g, Chenpi 50g and Wuzhimaotao 50g. The rest is the same as Example 1.

[0083] Comparative Example 2

[0084] This comparative example provides a fermented product, which differs from Example 1 in that the comparative example does not include tangerine peel, the weight ratio of dried orange peel and five-fingered ginseng peach remains unchanged, and the total weight is the same as the total weight of tangerine peel, dried orange peel and five-fingered ginseng peach in Example 1, specifically as follows: 93.75g of dried orange peel and 56.25g of five-fingered ginseng peach. The rest is the same as Example 1.

[0085] Comparative Example 3

[0086] This comparative example provides a fermented product, which differs from Example 1 in that the comparative example does not include dried tangerine peel and five-fingered ginseng, and the total weight is the same as the total weight of the dried tangerine peel, dried tangerine peel and five-fingered ginseng in Example 1, specifically as follows: 150g of dried tangerine peel. The rest is the same as Example 1.

[0087] Comparative Example 4

[0088] This comparative example provides a fermented product, which is different from Example 1 in that the tangerine peel is replaced with tangerine peel, and the weight ratio of tangerine peel and five-fingered ginseng peach remains unchanged, and the total weight is the same as the total weight of tangerine peel, tangerine peel and five-fingered ginseng peach in Example 1, specifically as follows: 110g tangerine peel, 25g tangerine peel and 15g five-fingered ginseng peach. The rest is the same as Example 1.

[0089] Comparative Example 5

[0090] This comparative example provides a fermentation product, which is different from Example 1 in that the total mass of the fermentation agent remains unchanged, and only includes 0.15 g of Lactobacillus reuteri. The rest is the same as Example 1.

[0091] Comparative Example 6

[0092] This comparative example provides a fermentation product, which is different from Example 1 in that the total mass of the fermentation agent remains unchanged, and only includes 0.15 g of Bifidobacterium longum subsp. longum. The rest is the same as Example 1.

[0093] Experimental Example Evaluation of the efficacy of fermented products

[0094] 1 Experimental grouping and modeling

[0095] SPF male SD rats were kept in a strictly controlled environment at 20-22°C, 60-70% relative humidity, and a 12-hour lighting cycle, with lights on from 7:00 to 19:00 and darkness from 19:00 to 7:00. After one week of adaptive feeding, they were randomly divided into two groups, with 10 rats in the blank control group eating maintenance feed and 150 rats in the experimental group given high-calorie feed (maintenance feed + 15% sucrose + 15% lard). Food intake and body weight were recorded weekly. After two weeks of feeding, the rats given high-calorie feed were sorted according to body weight gain, and the 1 / 5 obesity-resistant rats with lower body weight gain were eliminated to screen out obesity-sensitive rats.

[0096] The screened obesity-sensitive rats were randomly divided into 12 groups according to body weight, with 10 rats in each group, namely, the model control group, the Example 1-5 group and the Comparative Example 1-6 group. The blank control group was given a maintenance feed, and the other groups were given a high-calorie feed; the Example 1-5 group and the Comparative Example 1-6 group were fed with 13 mL / kg of the corresponding fermentation product, and the blank control group and the model control group were fed with an equal amount of normal saline for 28 consecutive days. On the 29th day of feeding, the rats were anesthetized by intraperitoneal injection of 1% sodium pentobarbital (0.5 mL / 100 g), and blood was collected from the abdominal aorta to preserve serum and plasma.

[0097] Determination of dosage: The daily oral dosage of rats was calculated using the kilogram body weight conversion coefficient method. According to the calculation that an adult weighing 70 kg consumes 150 mL of oral solution per day, the crossover conversion coefficient is 6.25, and the daily oral dosage of rats should be (150 mL / 70 kg) × 6.25 = 13.39 mL / kg, which is convenient for calculation. In subsequent experiments, each group was gavaged at a dose of 13 mL / kg.

[0098] 2. Detection indicators

[0099] 2.1 Observation of general indicators: Record the basic conditions of the hair, mental state, etc. of each group of rats after administration;

[0100] 2.2 Fat coefficient: After the experiment, the body fat (testicular and perirenal adipose tissue) of the rats was weighed and the fat coefficient was calculated. Fat coefficient (%) = body fat tissue weight (g) / body weight (g) × 100%;

[0101] 2.3 Blood lipid level: Serum total cholesterol (TC) and triglyceride (TG) concentrations were measured according to the kit instructions;

[0102] 2.4 Spleen-strengthening index: refer to the kit instructions to measure the gastrin (GAS) content in serum, motilin (MTL) and ghrelin content in plasma;

[0103] 2.5 Dehumidification index: Measure the plasma albumin (ALB) content and serum aldosterone (ALD) content according to the kit.

[0104] TG kit, TC kit, and ALB kit were purchased from Shanghai Yuanye Biotechnology Co., Ltd. with catalog numbers R21740, R21734, and R21617, respectively; GAS kit, MTL kit, and Ghrelin kit were purchased from Shanghai Keaibo Biotechnology Co., Ltd. with catalog numbers CB10835-Ra, CB10833-Ra, and CB12984-Ra; ALD kit was purchased from Shanghai Jining Biotechnology Co., Ltd. with catalog number JN6278.

[0105] 3 Results Analysis

[0106] 3.1 General indicators: After modeling, the rats showed dry and dull hair, fatigue, lack of activity, lack of energy, obesity, and loose feces. After the fermentation products of experimental example 1-5 groups and comparative example 1-6 groups were given, the above symptoms were improved to a certain extent, approaching the blank control group.

[0107] 3.2 Fat coefficient and blood lipid levels: The body fat weight, fat coefficient and blood lipid levels of each group are shown in Tables 1 and 2.

[0108] Table 1 Body fat weight and fat coefficient of rats in each group

[0109] Group Body fat weight (g) Fat percentage (%) Blank control group 10.24±1.55h 3.27±0.51f Model control group 28.18±1.59a 6.26±0.40a Example 1 12.64±1.04g 3.89±0.43e Example 2 13.39±1.57fg 4.06±0.52e Example 3 14.14±0.85f 4.14±0.18e Example 4 13.58±2.19fg 4.00±0.63e Example 5 12.98±1.87fg 3.94±0.55e Comparative Example 1 20.12±1.31d 5.37±0.42bcd Comparative Example 2 24.50±2.39b 5.70±0.50b Comparative Example 3 23.12±1.04c 5.56±0.24bc Comparative Example 4 22.06±1.24c 5.47±0.41bcd Comparative Example 5 18.95±1.52de 5.19±0.49d Comparative Example 6 18.83±0.98e 5.22±0.33cd

[0110] Note: Different lowercase letters in the same column indicate significant differences (P<0.05).

[0111] Table 2 Blood lipid levels of rats in each group

[0112]

[0113]

[0114] Note: Different lowercase letters in the same column indicate significant differences (P<0.05).

[0115] The model group rats showed physical changes such as mental depression, fatigue, poor appetite, dry and yellow fur, and sticky feces, which were consistent with the characteristics of spleen deficiency and dampness obstruction syndrome, and the body fat weight and fat index of the model control group rats in Table 1 were significantly higher than those of the blank control group. Compared with the model control group, the body fat weight and fat index of the Example 1-5 group and the Comparative Example 1-8 group were significantly reduced, indicating that the composition selected by the present invention has the effect of removing dampness and reducing weight.

[0116] Compared with the Example 1-5 group, the body fat weight and fat coefficient of the rats in the comparative example 1-5 group were significantly higher than those in the Example 1-5 group, indicating that tangerine peel, tangerine peel and five-fingered ginseng are indispensable components in the prescription, and the lack or replacement of any one variety will significantly reduce the overall dampness-removing and weight-reducing effect of the prescription, and the three must have a certain weight ratio to maximize the dampness-removing and weight-reducing effect; compared with the Example 1-5 group, the fat coefficient and the body fat number of the comparative example 6-8 group were significantly increased, indicating that the fermentation strain types and strain numbers selected by the Example 1-5 group were the optimal strain combinations for fermenting five-fingered ginseng, tangerine peel and tangerine peel.

[0117] Blood lipid metabolism is closely related to the development of spleen deficiency and dampness stagnation syndrome obesity. Obese people have high visceral fat content, which decomposes and produces a large amount of free fatty acids to enter the liver, promote TG synthesis, and excessive fat deposition can change lipoprotein lipase activity and increase TC content. Spleen deficiency, abnormal transportation and transformation, transformation into cream and fat phlegm, water and dampness stop gathering, causing abnormal lipid metabolism. "Wetness" is most likely to hurt the spleen, and the spleen is trapped, and spleen deficiency is easy to produce "wetness". Weak spleen qi cannot be used for the stomach to carry its body fluid, which causes water and dampness stagnation, causing water and dampness metabolic disorder. Dampness is long-term, sticky and turbid, blocking the qi machine, and abnormal blood lipid metabolism affects the transportation and transformation of the spleen. As shown in Table 2, there are significant differences in TG and TC content between blank control group rats and model control group rats, indicating that the blood lipid metabolism of model control group rats is abnormal, and the TC and TG concentrations of Example 1-5 group and Comparative Example 1-8 group are significantly lower than those of the model control group, indicating that the fermented product prepared by the present invention reduces TC and TG content, restores lipid metabolism in obese rats, and achieves the effect of strengthening the spleen, removing dampness and losing weight.

[0118] Compared with Example 1-5, the TG and TC concentrations of Comparative Example 1-8 were significantly higher than those of Example 1-5, indicating that the raw materials, raw material ratios, fermentation strain types and quantities selected by the present invention have the best effect in reducing the blood lipid levels of obese rats with spleen deficiency and dampness resistance type.

[0119] 3.3 Spleen-strengthening effect

[0120] "The spleen governs transportation and transformation" is closely related to the digestive system. Gastrointestinal hormone disorders are one of the main pathological mechanisms of spleen deficiency. GAS participates in gastric acid secretion and has the function of repairing gastric mucosa. MTL has the function of promoting gastrointestinal motility and transportation. Ghrelin can promote gastric emptying, accelerate small intestine and colon motility, and thus regulate food intake and energy metabolism.

[0121] Comparison of serum GAS, plasma MTL and Ghrelin contents of rats in each group is shown in Table 3. From the results, the model control group had significantly lower GAS, MTL and Ghrelin contents than the blank control group, indicating abnormal gastric acid secretion and abnormal digestive function; compared with the model control group, the GAS, MTL and Ghrelin contents of Example 1-5 groups and Comparative Example 1-6 groups were significantly increased, although they did not reach the numerical level of the blank control group, but also alleviated gastrointestinal hormone disorder and improved gastrointestinal function, indicating that the fermentation product of the present invention exerts a spleen-strengthening effect by regulating gastrointestinal hormones.

[0122] Table 3 Serum GAS, plasma MTL and Ghrelin levels in rats in each group

[0123]

[0124] Note: Different lowercase letters in the same column indicate significant differences (P<0.05).

[0125] 3.4 Dehumidification effect:

[0126] ALB and ALD are objective indicators of water metabolism. ALB has a hyperosmotic effect and maintains the water balance between tissues and blood vessels. Increasing ALB can improve abnormal water metabolism. ALD is an important regulatory factor for electrolyte excretion. Its function is to retain sodium and excrete potassium. It plays an important role in maintaining the stability of the internal environment and can promote the reabsorption of water by renal tissue.

[0127] Comparison of serum ALD and plasma ALB contents of rats in each group is shown in Table 4. Compared with the blank control group, the ALD content of the model control group was significantly increased, and the ALB content was significantly decreased, indicating that there was a phenomenon of edema caused by abnormal water metabolism. Compared with the model control group, the ALB of Example 1-5 groups and Comparative Example 1-6 groups were significantly increased, and the ALD was significantly decreased, indicating that the fermentation product of the present invention can promote water elimination, regulate water and moisture metabolism disorders, and exert a dehumidifying effect.

[0128] Table 4 Serum ALD and plasma ALB levels in rats in each group

[0129]

[0130]

[0131] Note: Different lowercase letters in the same column indicate significant differences (P<0.05).

[0132] In summary, the fermentation product of the present invention achieves the weight loss effect by reducing the TG and TC contents; reduces the ALD content and increases the ALB content to achieve the dampness removal effect; increases the MTL, GAS and Ghrelin contents, regulates the gastrointestinal function, and thus achieves the spleen strengthening effect; the three raw materials selected in the present invention cooperate synergistically to give full play to their respective effects in the composition, and the composition obtained after fermentation can alleviate the symptoms of simple obesity of spleen deficiency and dampness obstruction type.

[0133] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions of the technical solution of the present invention by ordinary technicians in this field do not deviate from the essence and scope of the technical solution of the present invention.

Claims

1. A fermented product for alleviating simple obesity of spleen deficiency and dampness obstruction type, characterized in that: The invention comprises 80-150 parts of dried orange peel, 15-40 parts of dried orange peel, 5-30 parts of quinquefolius, 0.1-0.22 parts of leavening agent and 8-18 parts of enzyme preparation in parts by weight.

2. The fermented product for alleviating simple obesity of spleen deficiency and dampness resistance type according to claim 1, characterized in that: The invention comprises 90-130 parts of dried orange peel, 20-30 parts of dried orange peel, 10-20 parts of quinquefolius, 0.12-0.18 parts of leavening agent and 10-15 parts of enzyme preparation in parts by weight.

3. The fermented product for alleviating simple obesity of spleen deficiency and dampness resistance type according to claim 1 or 2, characterized in that: The fermentation agent comprises 0.07-0.16 parts of Lactobacillus reuteri and 0.03-0.06 parts of Bifidobacterium longum subsp. longum.

4. The fermented product for alleviating simple obesity of spleen deficiency and dampness resistance type according to claim 1 or 2, characterized in that: The enzyme preparation comprises 5-11 parts of cellulase and 3-7 parts of pectinase.

5. The method for preparing a fermented product for alleviating simple obesity of spleen deficiency and dampness resistance type according to any one of claims 1 to 4, characterized in that: The steps include: (1) mixing tangerine peel, dried orange peel, and citrus aurantium with water to obtain a mixed solution; (2) adding an enzyme preparation to the mixed solution for enzymolysis, then performing microwave treatment, and adjusting the pH to 6-7 to obtain an enzymolysis treatment solution; (3) raising the temperature of the enzymatic hydrolysis solution to perform enzyme inactivation extraction treatment to obtain an enzyme inactivation extract solution; (4) when the temperature of the enzyme-inactivated extract drops to 30-40° C., adding a fermentation agent for fermentation to obtain a fermentation liquid; (5) The fermentation liquid is centrifuged, and the supernatant is the fermentation liquid.

6. The method for preparing the fermentation composition according to claim 5, characterized in that: In the step (2), the enzymolysis temperature is 50-60° C., the enzymolysis time is 1-3 h, the microwave time is 3-12 min, and the microwave power is 500-900 W.

7. The method for preparing the fermentation composition according to claim 5, characterized in that: The enzyme inactivation extraction temperature in step (3) is 90-115° C., and the enzyme inactivation extraction time is 40-90 min; the fermentation temperature in step (4) is 35-42° C., and the fermentation time is 10-50 h.

8. A fermented preparation for strengthening the spleen, removing dampness and reducing weight, characterized in that: The fermentation preparation comprises: the fermentation composition according to any one of claims 1 to 4 and auxiliary materials.

9. The fermentation preparation according to claim 8, characterized in that The auxiliary material comprises at least one of maltodextrin, mannose, skim milk powder, cyclodextrin, glycerol and starch.

10. Use of the fermentation composition according to any one of claims 1-4, the fermentation composition obtained by the preparation method according to any one of claims 5-7, or the fermentation preparation according to any one of claims 8-9 in the preparation of medicines, health foods, foods, etc. for treating simple obesity of spleen deficiency and dampness resistance type.

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