Leymus chinensis water culture nutrient solution as well as preparation method and application thereof

Abstract

The invention discloses a Chinese wildrye hydroponic nutrient solution as well as a preparation method and application thereof, and belongs to the technical field of plant cultivation. Each liter of the nutrient solution comprises the following components: 80 to 120 mg of ammonium nitrate, 45 to 55 mg of sodium dihydrogen phosphate dihydrate, 80 to 90 mg of potassium sulfate, 110 to 115 mg of anhydrous calcium chloride, 400 to 410 mg of magnesium sulfate heptahydrate, 1.5 to 2.0 mg of manganese chloride tetrahydrate, 0.08 to 0.10 mg of ammonium molybdate tetrahydrate, 1.1 to 1.2 mg of boric acid, 0.02 to 0.05 mg of zinc sulfate heptahydrate, 0.02 to 0.05 mg of copper sulfate pentahydrate, 15 to 35 mg of ferrous sulfate heptahydrate and 20 to 50 mg of disodium ethylene diamine tetraacetate. The hydroponic nutrient solution provided by the invention meets the nutritional requirements of leymus chinensis, seedlings grow quickly and vigorously by utilizing the nutrient solution, the biomass and nutrient content of the overground part are remarkably increased, and the high-quality pasture characteristics of the leymus chinensis are ensured.

Description

Technical Field

[0001] The invention relates to the technical field of plant cultivation, and in particular to a hydroponic nutrient solution for growing Leymus chinensis, and a preparation method and application thereof. Background Art

[0002] Leymus chinensis (Trin.ex Bunge) Tzvelev, also known as alkali grass, is a perennial rhizome grass of the genus Leymus in the Poaceae family. It has a strong adaptability to different growth environments and is one of the most salt-tolerant plants among non-halophytes. It also has characteristics such as drought resistance and cold resistance. Leymus chinensis is an advantageous forage and ecological grass in my country. It has the advantages of good palatability and high nutritional value. Its perennial habit and salt, drought and cold resistance make it an important forage resource.

[0003] Hydroponics is a type of soilless plant cultivation method and is the abbreviation of nutrient solution culture. Hydroponics is to fix plants in nutrient solution, and the nutrient solution replaces the soil to provide water and nutrients to the plants. Artificially matched with appropriate temperature and humidity and reasonable light cycle, the plants can grow normally and complete their life cycle. Compared with soil cultivation, hydroponics shows more prominent advantages: the plant roots are completely immersed in the nutrient solution, which can induce the vigorous growth of the plant roots and provide favorable conditions for the accumulation of biomass in the aboveground part of the plant; hydroponics can maximize the absorption and utilization of nutrients in the nutrient solution by plants, which can increase plant yields and thus improve economic benefits; hydroponics can control the culture conditions through automation and monitor the growth status of plants in real time, saving labor costs; from the perspective of ecological protection, hydroponics can not only reduce problems such as reduced soil fertility, but also reduce the adverse effects of pesticides and fertilizers on the environment. Therefore, the development of hydroponic technology suitable for the growth of sheep grass is expected to solve the current problem of insufficient yield and quality of sheep grass populations.

[0004] The preparation of hydroponic nutrient solution is the key to obtaining high-yield and high-quality forage. For example, Hoagland's solution is the most commonly used formula in plant nutrient solution, which is beneficial to plant reproduction and growth and development. Patent document CN106810384A discloses a nutrient solution suitable for hydroponics of crops such as forage that require less fertilizer during growth. Each 1000mL is composed of the following ingredients: 130mg urea, 20mg potassium dihydrogen phosphate, 60mg potassium chloride, 40mg magnesium sulfate, 2mg zinc sulfate, 3.5mg copper chloride, 4mg ferrous sulfate, 1.5mg manganese sulfate, 2mg calcium chloride, 2.5mg borax, 0.02mg sodium molybdate and 2mL rare earth nutrient solution, and the balance is water. Patent document CN117964426A discloses a hydroponic nutrient solution for cultivating forage germplasm resources, which is made of the following components: calcium nitrate tetrahydrate, potassium nitrate, ammonium dihydrogen phosphate, magnesium sulfate heptahydrate, disodium iron ethylenediaminetetraacetate, boric acid, manganese sulfate, copper sulfate, zinc sulfate, sodium molybdate, and composite fermentation liquid. The nutrient solution is used for cultivating seedlings of wheatgrass, oats, and ryegrass to promote plant growth and prevent root rot.

[0005] Different types of plants have different nutritional requirements for growth. In order to adapt to the hydroponic technology of sheep fescue, it is necessary to optimize the formula of the hydroponic nutrient solution. Summary of the invention

[0006] The object of the present invention is to provide a hydroponic nutrient solution and a hydroponic method suitable for the growth of Leymus chinensis, especially for cultivation from the seedling stage to the tillering stage, so as to promote the improvement of the yield and quality of Leymus chinensis.

[0007] To achieve the above object, the present invention adopts the following technical solution:

[0008] The invention provides a hydroponic nutrient solution for sheep fescue. The nutrient solution comprises, per liter, 80-120 mg of ammonium nitrate, 45-55 mg of sodium dihydrogen phosphate dihydrate, 80-90 mg of potassium sulfate, 110-115 mg of anhydrous calcium chloride, 400-410 mg of magnesium sulfate heptahydrate, 1.5-2.0 mg of manganese chloride tetrahydrate, 0.08-0.10 mg of ammonium molybdate tetrahydrate, 1.1-1.2 mg of boric acid, 0.02-0.05 mg of zinc sulfate heptahydrate, 0.02-0.05 mg of copper sulfate pentahydrate, 15-35 mg of ferrous sulfate heptahydrate and 20-50 mg of disodium ethylenediaminetetraacetic acid, and the pH value is 5.0-5.5.

[0009] In the formula provided by the present invention, ammonium nitrate mainly provides a nitrogen source, sodium dihydrogen phosphate provides phosphorus, and potassium sulfate provides potassium, which provide the necessary macroelements for the growth and development of Leymus chinensis, and ensure the accumulation of aboveground substances in the Leymus chinensis. The addition of calcium chloride, magnesium sulfate and potassium sulfate ensures the normal supply of calcium, magnesium and sulfur elements in the development of Leymus chinensis. The results of the study show that the growth of Leymus chinensis cultivated with the hydroponic nutrient solution provided by the present invention tends to accumulate aboveground biomass and nutrients, significantly improving yield and forage quality.

[0010] Preferably, the composition per liter of nutrient solution includes: 110-120 mg of ammonium nitrate, 48-52 mg of sodium dihydrogen phosphate dihydrate, 85-90 mg of potassium sulfate, 110-112 mg of anhydrous calcium chloride, 400-405 mg of magnesium sulfate heptahydrate, 1.8-1.9 mg of manganese chloride tetrahydrate, 0.08-0.10 mg of ammonium molybdate tetrahydrate, 1.1-1.2 mg of boric acid, 0.04-0.05 mg of zinc sulfate heptahydrate, 0.03-0.04 mg of copper sulfate pentahydrate, 30-35 mg of ferrous sulfate heptahydrate, and 45-50 mg of disodium ethylenediaminetetraacetic acid.

[0011] More preferably, the composition per liter of nutrient solution includes: 114.25 mg of ammonium nitrate, 50.375 mg of sodium dihydrogen phosphate dihydrate, 89.25 mg of potassium sulfate, 110.75 mg of anhydrous calcium chloride, 405 mg of magnesium sulfate heptahydrate, 1.875 mg of manganese chloride tetrahydrate, 0.093 mg of ammonium molybdate tetrahydrate, 1.168 mg of boric acid, 0.044 mg of zinc sulfate heptahydrate, 0.039 mg of copper sulfate pentahydrate, 34.75 mg of ferrous sulfate heptahydrate, and 46.5 mg of disodium ethylenediaminetetraacetic acid.

[0012] Compared with the commonly used hydroponic nutrient solution (Hoagland nutrient solution), the plant height of Sheepgrass cultured using the above nutrient solution increased significantly and continued to maintain its advantage, the increase in root length was significantly reduced, and the aboveground biomass and nutrient content were significantly increased.

[0013] The present invention also provides a method for preparing the above-mentioned hydroponic nutrient solution of Leymus chinensis, comprising the following steps:

[0014] (1) ammonium nitrate, sodium dihydrogen phosphate dihydrate, potassium sulfate, anhydrous calcium chloride, and magnesium sulfate heptahydrate are dissolved in water to prepare mother liquors I, II, III, IV, and V, respectively, for later use; manganese chloride tetrahydrate, ammonium molybdate tetrahydrate, boric acid, zinc sulfate heptahydrate, and copper sulfate pentahydrate are mixed and dissolved in water to prepare mother liquor VI, for later use; disodium ethylenediaminetetraacetate is added to boiling water until it is completely dissolved, and then ferrous sulfate heptahydrate is added, and the solution is heated and stirred until it turns brownish yellow, to prepare mother liquor VII, for later use;

[0015] (2) taking mother liquor I, mother liquor II, mother liquor III, mother liquor IV, mother liquor V, mother liquor VI and mother liquor VII in proportion, mixing them, adding water to make up the volume, and adjusting the pH value to 5.0-5.5 with concentrated hydrochloric acid to obtain the nutrient solution for hydroponic culture of Leymus chinensis.

[0016] Preferably, in step (1), the concentrations of mother liquor I, mother liquor II, mother liquor III, mother liquor IV and mother liquor V are 91.4 g / L, 40.3 g / L, 71.4 g / L, 88.6 g / L and 324 g / L respectively; the concentration of manganese dichloride tetrahydrate in mother liquor VI is 1.5 g / L, the concentration of ammonium molybdate tetrahydrate is 0.074 g / L, the concentration of boric acid is 0.934 g / L, the concentration of zinc sulfate heptahydrate is 0.035 g / L, and the concentration of copper sulfate pentahydrate is 0.031 g / L; the concentration of ferrous sulfate heptahydrate in mother liquor VII is 6.95 g / L, and the concentration of disodium ethylenediaminetetraacetate is 9.306 g / L; in step (2), the mother liquors I-VI and mother liquor VII are mixed in a volume ratio of 1:4 and dissolved in water.

[0017] The hydroponic nutrient solution for Leymus chinensis in the present invention should be prepared and used immediately to prevent the nutrient solution from being precipitated after being left for a long time, thereby affecting the cultivation effect.

[0018] The present invention's research shows that lowering the pH value can promote the dissolution of elements such as magnesium ions and calcium ions, and a pH value below 6 is helpful for plants to absorb nutrient elements.

[0019] The present invention utilizes the nutrient solution to cultivate Leymus chinensis, thereby significantly increasing the biomass of Leymus chinensis and ensuring the high-quality forage characteristics of Leymus chinensis, which is of great significance to expanding forage cultivation and protecting grassland ecological environment.

[0020] The present invention provides the use of the hydroponic nutrient solution of Leymus chinensis as a hydroponic nutrient solution in the seedling stage and / or tillering stage of Leymus chinensis. The hydroponic nutrient solution of Leymus chinensis provided by the present invention meets the requirements of Leymus chinensis hydroponic culture process, especially the requirements of seedlings for nutrient elements. The Leymus chinensis seedlings using the culture solution of the present invention grow rapidly and vigorously.

[0021] The present invention also provides a hydroponic method for growing Leymus chinensis, which comprises: transplanting the germinated Leymus chinensis seedlings into a hydroponic facility containing the Leymus chinensis hydroponic nutrient solution, wherein the hydroponic conditions are: 22-26°C, 16h light / 8h dark; and replacing the nutrient solution once a week.

[0022] Preferably, the method for cultivating Leymus chinensis seedlings comprises: selecting Leymus chinensis seeds with full grains for shelling, inoculating on 1 / 2MS culture medium, at 22-26°C, 16h light / 8h dark, until the seedlings grow to about 7-10cm.

[0023] When the seedlings grow to a certain size, they are transplanted to ensure that they have enough space for growth. As a preference, the seedlings are transplanted to a hydroponic facility with a seedling transplanting density of 0.05 plants / cm 2 Specifically, one liter of nutrient solution was added to the 8cm×12cm×11cm hydroponic facility.

[0024] Preferably, the hydroponic facility is made of opaque material, and the nutrient solution is partially shielded from light to prevent algae growth.

[0025] The present invention has the following beneficial effects:

[0026] (1) The hydroponic nutrient solution provided by the present invention has reasonable composition, which meets the demand for nutrients during the growth of Leymus chinensis, especially from the seedling stage to the tillering stage. The Leymus chinensis seedlings using the culture solution of the present invention grow rapidly, grow vigorously, and significantly increase the aboveground biomass and nutrient content, ensuring the high-quality forage characteristics of Leymus chinensis. The use of the culture solution of the present invention can reduce the use of chemical fertilizers and pesticides, which plays a good role in protecting the ecological balance.

[0027] (2) The hydroponic technology provided by the present invention can expand the planting range of leymus chinensis, accelerate the growth of leymus chinensis, improve the production quality of leymus chinensis, and reduce manual operations and their costs; the hydroponic method can avoid the occurrence of soil-borne diseases and insect pests, ensuring a safe environment for the growth of leymus chinensis. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 These are seedlings of Leymus chinensis seeds 7 days after germination.

[0029] Figure 2 The growth conditions of Leymus chinensis hydroponically cultivated for 0-1 week in different embodiments are shown.

[0030] Figure 3 The growth conditions of Leymus chinensis hydroponically cultivated for 2-5 weeks in different embodiments are shown.

[0031] Figure 4 The growth conditions of Leymus chinensis hydroponically grown in different embodiments for 6-8 weeks are shown.

[0032] Figure 5 The present invention provides a statistical analysis of the growth trend and plant height increase of Leymus chinensis hydroponically cultivated for 1-8 weeks in different embodiments.

[0033] Figure 6 The present invention provides a statistical analysis of the growth trend and root length increase of Leymus chinensis hydroponically cultivated for 1-8 weeks in different embodiments.

[0034] Figure 7 The figure shows the accumulation of nutrients of Leymus chinensis after 8 weeks of hydroponics in different embodiments, wherein A is neutral detergent fiber (NDF, %); B is acid detergent fiber (ADN, %). DETAILED DESCRIPTION

[0035] The present invention will be further described below in conjunction with specific embodiments. The following embodiments are only used to illustrate the present invention and are not intended to limit the scope of application of the present invention. Without departing from the spirit and essence of the present invention, modifications or replacements made to the inventive method, steps or conditions all belong to the scope of the present invention.

[0036] Unless otherwise specified, the experimental methods used in the following examples are all conventional methods; the materials and reagents used are reagents and materials that can be obtained from commercial channels unless otherwise specified.

[0037] In the following examples, the seeds of Leymus chinensis were obtained from the Shaerqin breeding base in Hohhot. 1 / 2MS culture medium was purchased from Coolaber, item number PM1011.

[0038] Example 1: Formula of mother solution of hydroponic nutrient solution A of Leymus chinensis

[0039] The formula of mother solution A of hydroponic nutrient solution of Leymus chinensis is as follows:

[0040] Mother solution I: Dissolve 64g NH4NO3 in water and make up to 1L;

[0041] Mother solution II: 40.3 g NaH2PO4·2H2O is dissolved in water and the volume is adjusted to 1 L;

[0042] Mother solution III: 71.4 g K2SO4 is dissolved in water and the volume is adjusted to 1 L;

[0043] Mother solution IV: 88.6 g of anhydrous CaCl2 is dissolved in water and the volume is adjusted to 1 L;

[0044] Mother solution V: 324 g MgSO4·7H2O dissolved in water and volume adjusted to 1 L;

[0045] Mother solution VI: 1.5 g MnCl2·4H2O, 0.074 g (NH4)6Mo7O 24 ·4H2O, 0.934gH3BO3, 0.035gZnSO4·7H2O, 0.031gCuSO4·5H2O are dissolved in water and the volume is adjusted to 1L;

[0046] Mother solution VII: Dissolve 3.5 g FeSO4·7H2O and 4.7 g EDTA-Na2 in water and make up to 1 L.

[0047] Note: When preparing mother solution VII, first add EDTA-Na2 into boiling ddH2O until it is completely dissolved, then add FeSO4·7H2O, and stir continuously while heating (2-3 hours) until the solution turns brown.

[0048] Example 2: Formula of mother solution of hydroponic nutrient solution B for Leymus chinensis

[0049] The formula of the mother solution of the hydroponic nutrient solution B for Leymus chinensis differs from that of Example 1 in that, in mother solution VII, 6.95 g of FeSO4·7H2O and 9.306 g of EDTA-Na2 are dissolved in water and the volume is fixed to 1 L. The rest is the same as that of Example 1.

[0050] Example 3: Formula of mother solution of hydroponic nutrient solution C for Leymus chinensis

[0051] The formula of the mother solution of the hydroponic nutrient solution C for Leymus chinensis differs from that of Example 1 in that: mother solution I: 91.4 g NH4NO3 is dissolved in water and the volume is fixed to 1 L; mother solution VII: 6.95 g FeSO4·7H2O and 9.306 g EDTA-Na2 are dissolved in water and the volume is fixed to 1 L. The rest is the same as Example 1.

[0052] Example 4: Preparation of Leymus chinensis hydroponic nutrient solution

[0053] Experimental group: The mother solutions of Examples 1-3 were used to prepare hydroponic nutrient solutions of Leymus chinensis, respectively. The method was as follows: 5 mL of each of mother solutions I-VI and 20 mL of solution VII were added to every 4 L of hydroponic nutrient solution, water was added to make the volume to 4 L and the solution was mixed thoroughly, and the pH value was adjusted to 5.0-5.5 with concentrated hydrochloric acid.

[0054] Control group: Hoagland nutrient solution, purchased from Phygene, catalog number PH1782. Each liter of nutrient solution contains 607 mg potassium sulfate, 115 mg diammonium phosphate, 493 mg magnesium sulfate, 20 mg EDTA sodium iron salt, 2.86 mg ferrous sulfate, 4.5 mg borax, 2.13 mg manganese sulfate, 0.05 mg copper sulfate, 0.22 mg zinc sulfate, 0.02 mg ammonium sulfate and calcium salt.

[0055] Example 5: Cultivation of Leymus chinensis by hydroponic method

[0056] 1. The detailed steps of the hydroponic method of sheep grass are as follows:

[0057] (1) Selection of equipment for cultivating sheep fescue: Hydroponics uses black hydroponic boxes and opaque hydroponic troughs, and black floats are made to prevent subsequent growth of algae that affects plant growth.

[0058] (2) Treatment of Leymus chinensis seeds: Select seeds that are full and in good condition, dehull them, and germinate them on 1 / 2MS culture medium. The culture conditions are: 26°C, 16 h light / 8 h dark. The seedlings after germination for 7 days are as follows: Figure 1 shown.

[0059] (3) The germinated seedlings were transplanted into the hydroponic nutrient solution prepared in Example 4 at a transplanting density of 0.05 plants / cm 2 The culture conditions are: 26℃, 16h light / 8h dark. The nutrient solution needs to be replaced once a week.

[0060] (4) Twelve stalks of Leymus chinensis were cultured in each example, and the growth of the stalks of Leymus chinensis within 0-8 weeks was as follows: Figure 2-4 The plant height and root length of Leymus chinensis were measured every week, and the difference between the plant height and root length measured weekly and the plant height and root length of Leymus chinensis during the initial hydroponic culture was counted. The growth of plant height and root length after hydroponic culture of Leymus chinensis was analyzed. The statistical results are shown in Figure 5 , Figure 6 As shown, n = 12, **** represents P < 0.0001, *** represents P < 0.001, ** represents P < 0.01, and * represents P < 0.05.

[0061] (5) Samples of 8-week-old sheepgrass were taken from 3 cm above the ground, dried at 75°C for 48 h, and then the nutrient content indicators neutral detergent fiber (NDF, %) and acid detergent fiber (ADF, %) were measured. The measurement method was referred to (Zhang Liying. Feed Analysis and Feed Quality Detection Technology [M]. Beijing: China Agricultural University Press, 2016.). The statistical results are as follows: Figure 7 As shown, n = 6, *** represents P < 0.001.

[0062] 2. Results Analysis

[0063] After analyzing the growth trends of the four groups of Leymus chinensis, it was found that there was no significant difference in the aboveground plant height of the Leymus chinensis cultivated in each embodiment in the initial 1-5 weeks of hydroponic culture. After 6 weeks of hydroponic culture, the plant height of the Leymus chinensis cultivated in Example 3 increased significantly compared with the control group and continued to maintain its advantage. In addition, the increase in root length of the Leymus chinensis cultivated in Example 3 was significantly less than that of the other embodiments, indicating that the growth of the Leymus chinensis cultivated in Example 3 was more biased towards the aboveground part, and the aboveground biomass increased significantly.

[0064] Analysis of the aboveground nutrient components of the sheep grass cultivated in different embodiments also confirmed that the sheep grass cultivated in Example 3 had higher NDF and ADF accumulation and better forage quality.

[0065] Thus, the present invention obtains a formula of a hydroponic nutrient solution for Leymus chinensis and a preparation method thereof, and the distribution ratio of each component is based on Example 3 as the optimal addition concentration.

Claims

1. A nutrient solution for hydroponic cultivation of Leymus chinensis, characterized in that: The composition of each liter of nutrient solution includes: ammonium nitrate 80-120 mg, sodium dihydrogen phosphate dihydrate 45-55 mg, potassium sulfate 80-90 mg, anhydrous calcium chloride 110-115 mg, magnesium sulfate heptahydrate 400-410 mg, manganese chloride tetrahydrate 1.5-2.0 mg, ammonium molybdate tetrahydrate 0.08-0.10 mg, boric acid 1.1-1.2 mg, zinc sulfate heptahydrate 0.02-0.05 mg, copper sulfate pentahydrate 0.02-0.05 mg, ferrous sulfate heptahydrate 15-35 mg, and disodium ethylenediaminetetraacetic acid 20-50 mg.

2. The hydroponic nutrient solution for Leymus chinensis according to claim 1, characterized in that: The composition of each liter of nutrient solution includes: ammonium nitrate 110-120 mg, sodium dihydrogen phosphate dihydrate 48-52 mg, potassium sulfate 85-90 mg, anhydrous calcium chloride 110-112 mg, magnesium sulfate heptahydrate 400-405 mg, manganese chloride tetrahydrate 1.8-1.9 mg, ammonium molybdate tetrahydrate 0.08-0.10 mg, boric acid 1.1-1.2 mg, zinc sulfate heptahydrate 0.04-0.05 mg, copper sulfate pentahydrate 0.03-0.04 mg, ferrous sulfate heptahydrate 30-35 mg, and disodium ethylenediaminetetraacetic acid 45-50 mg.

3. The hydroponic nutrient solution for Leymus chinensis according to claim 1, characterized in that: The composition of each liter of nutrient solution includes: 114.25 mg of ammonium nitrate, 50.375 mg of sodium dihydrogen phosphate dihydrate, 89.25 mg of potassium sulfate, 110.75 mg of anhydrous calcium chloride, 405 mg of magnesium sulfate heptahydrate, 1.875 mg of manganese chloride tetrahydrate, 0.093 mg of ammonium molybdate tetrahydrate, 1.168 mg of boric acid, 0.044 mg of zinc sulfate heptahydrate, 0.039 mg of copper sulfate pentahydrate, 34.75 mg of ferrous sulfate heptahydrate, and 46.5 mg of disodium ethylenediaminetetraacetic acid.

4. The method for preparing the hydroponic nutrient solution of Leymus chinensis according to any one of claims 1 to 3, characterized in that: The following steps are involved: (1) ammonium nitrate, sodium dihydrogen phosphate dihydrate, potassium sulfate, anhydrous calcium chloride, and magnesium sulfate heptahydrate are dissolved in water to prepare mother liquors I, II, III, IV, and V, respectively, for later use; manganese chloride tetrahydrate, ammonium molybdate tetrahydrate, boric acid, zinc sulfate heptahydrate, and copper sulfate pentahydrate are mixed and dissolved in water to prepare mother liquor VI, for later use; disodium ethylenediaminetetraacetate is added to boiling water until it is completely dissolved, and then ferrous sulfate heptahydrate is added, and the solution is heated and stirred until it turns brownish yellow, to prepare mother liquor VII, for later use; (2) taking mother liquor I, mother liquor II, mother liquor III, mother liquor IV, mother liquor V, mother liquor VI and mother liquor VII in proportion, mixing them, adding water to make up the volume, and adjusting the pH value to 5.0-5.5 with concentrated hydrochloric acid to obtain the nutrient solution for hydroponic culture of Leymus chinensis.

5. The preparation method according to claim 4, characterized in that: In step (1), the concentrations of mother liquor I, mother liquor II, mother liquor III, mother liquor IV and mother liquor V are 91.4 g / L, 40.3 g / L, 71.4 g / L, 88.6 g / L and 324 g / L respectively; the concentration of manganese dichloride tetrahydrate in mother liquor VI is 1.5 g / L, the concentration of ammonium molybdate tetrahydrate is 0.074 g / L, the concentration of boric acid is 0.934 g / L, the concentration of zinc sulfate heptahydrate is 0.035 g / L, and the concentration of cupric sulfate pentahydrate is 0.031 g / L; the concentration of ferrous sulfate heptahydrate in mother liquor VII is 6.95 g / L, and the concentration of disodium ethylenediaminetetraacetate is 9.306 g / L; in step (2), each of mother liquors I to VI is mixed with mother liquor VII in a volume ratio of 1:4 and dissolved in water.

6. Use of the hydroponic nutrient solution for Leymus chinensis as described in any one of claims 1 to 5 as a hydroponic nutrient solution for Leymus chinensis in the seedling stage and / or tillering stage.

7. A method for hydroponically growing Leymus chinensis, characterized in that: The method comprises: transplanting the germinated Leymus chinensis seedlings into a hydroponic facility containing the Leymus chinensis hydroponic nutrient solution according to any one of claims 1 to 3, wherein the hydroponic conditions are: 22-26° C., 16 h light / 8 h dark, and the nutrient solution is replaced once a week.

8. The method for hydroponics of Leymus chinensis according to claim 7, characterized in that: The method for cultivating Leymus chinensis seedlings includes: selecting Leymus chinensis seeds with full grains for shelling, inoculating them on 1 / 2MS culture medium, at 22-26°C, 16h light / 8h dark, until the seedlings grow to about 7-10cm.

9. The method for hydroponics of Leymus chinensis according to claim 7, characterized in that: The seedling transplanting density is 0.05 plants / cm 2 .

10. The method for hydroponics of Leymus chinensis according to claim 7, characterized in that: The hydroponic facility is made of light-proof material.

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