A vector

Abstract

The present application relates to a carrier, comprising a body and a sealing member, the body is provided with a sample containing cavity and a sealing cavity, the sample containing cavity is communicated with the sealing cavity, the cavity wall of the sample containing cavity is a deformable wall, at least part of the sealing member can be sealed into the sealing cavity to at least compress the gas in the sealing cavity, and the deformable wall is protruded to tightly contact with a temperature adjusting device. The carrier provided by the present application can accelerate the heating and cooling speed of the reaction sample, and improve the amplification efficiency.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of in vitro diagnosis, in particular to a carrier. BACKGROUND

[0002] PCR (Polymerase Chain Reaction) refers to a molecular biology experiment method for in vitro enzymatic synthesis of specific DNA fragments, which mainly consists of three steps of repeated thermal cycles of high-temperature denaturation, low-temperature annealing and suitable temperature extension. Before the reaction sample is subjected to PCR amplification, it needs to be placed in a carrier, wherein the reaction sample is composed of collected throat swabs or nasal swabs and reaction samples for PCR amplification. During PCR amplification, the reaction sample needs to be heated by a heater and cooled by a cooling mechanism, so that the reaction sample is cycled in the stages of high-temperature denaturation, low-temperature annealing and suitable temperature extension.

[0003] Some carriers in the prior art are pipe structures such as ep pipe structures, the area of the carrier for containing the reaction sample has a large diameter, the uniform heating speed is slow, the temperature rising and falling speed of the reaction sample is slow, and the reaction tube is difficult to closely fit with the heating structure, which affects the temperature rising and falling speed. In addition, the area of the pipe carrier for containing the reaction sample has a large diameter, the uniform heating speed of the reaction sample is slow, and the temperature rising and falling speed of the reaction sample is slow. At the same time, the wall thickness of the pipe structure carrier is large, the heat transfer is slow, and the temperature rising and falling speed of the reaction sample is further slow.

[0004] In the prior art, the PCR tube is an open structure, the reaction sample is injected into the sample containing cavity through the opening of the PCR tube, and the gas in the sample containing cavity is also discharged from the opening. However, when the liquid injection port in the carrier is small, the gas is difficult to discharge, resulting in a large amount of residual gas in the carrier, thereby affecting the amplification efficiency. At the same time, the residual gas in the carrier and the gas separated out in the reaction sample during the amplification process are often mixed in the reaction sample, thereby affecting the detection result and the amplification efficiency.

[0005] In the prior art, in order to solve the problem that the carrier cannot closely fit with the heater, more reaction sample than required for normal amplification and detection is introduced into the carrier to make the carrier closely fit with the heater. On the one hand, introducing more reaction sample leads to slower temperature rising and falling speed, and on the other hand, for some extremely scarce reaction sample, it is not desirable to introduce more reaction sample. Introducing gas to make the reaction sample swell is also a method to make the carrier closely fit with the heater, but a separate gas pump or extrusion device is required for gas introduction, resulting in a large structure of the overall device and inconvenient operation. SUMMARY

[0006] The present application aims to provide a carrier to at least solve one of the above technical problems.

[0007] To achieve the above object, the application provides a carrier, comprising a body and a sealing member, the body is provided with a sample containing cavity and a sealing cavity, the sample containing cavity is communicated with the sealing cavity, at least part of the cavity wall of the sample containing cavity is a deformable wall, at least part of the sealing member can be inserted into the sealing cavity to compress the gas in the sealing cavity and make the deformable wall protrude out to tightly contact with a temperature adjusting device.

[0008] Optionally, the deformable wall is a deformable membrane.

[0009] Optionally, the sealing member can be inserted into the sealing cavity to compress the gas in the sealing cavity and the sample containing cavity.

[0010] Optionally, the body and / or the sample containing cavity is in a flat structure.

[0011] Optionally, the body comprises a first wall and a second wall arranged oppositely, and a side wall arranged between the first wall and the second wall, the first wall and / or the second wall comprises the deformable wall.

[0012] Optionally, the first wall and the second wall are both the deformable wall at least at positions opposite to the sample containing cavity.

[0013] Optionally, the side wall is made of transparent material.

[0014] Optionally, the first wall is the deformable wall at least at positions opposite to the sample containing cavity.

[0015] Optionally, the second wall and / or the side wall is made of transparent material.

[0016] Optionally, the second wall and the side wall are integrally formed.

[0017] Optionally, the thickness of the second wall is not more than 0.5 mm.

[0018] Optionally, the sealing member comprises a first plug column, the first plug column is sealingly arranged in the sealing cavity.

[0019] Optionally, the sealing member comprises a second plug column and a sealing part connected with each other, the sealing part is arranged around the outer periphery of the second plug column and is arranged in interval with the outer periphery of the second plug column, the outer periphery of the sealing part can sealingly contact with the cavity wall of the sealing cavity.

[0020] Optionally, the outer periphery of the sealing part is formed with a sealing convex ring, the sealing convex ring sealingly contacts with the cavity wall of the sealing cavity.

[0021] Optionally, the sealing member further comprises a positioning cap, the second plug column and the end of the sealing part are connected to the positioning cap, and the positioning cap is capable of abutting against the outer surface of the body.

[0022] Optionally, the sealing member is integrally formed with the body.

[0023] Optionally, the body further comprises an annular wall, the annular wall is connected to the side wall, and the space in the annular wall forms the sealing cavity.

[0024] Optionally, the sealing member is in interference fit with the sealing cavity.

[0025] Optionally, the flat structure refers to that the size of the sample containing cavity or the body in the direction perpendicular to the thickness direction is greater than the size in the thickness direction.

[0026] Optionally, the ratio of the size of the sample containing cavity or the body in the direction perpendicular to the thickness direction to the size in the thickness direction is greater than 5:1.

[0027] Optionally, the ratio is 50:1-100:1.

[0028] Optionally, the body is further provided with a liquid inlet channel, and the sample containing cavity and the sealing cavity are communicated through the liquid inlet channel.

[0029] Optionally, the upper end of the liquid inlet channel is connected with the sealing cavity, and the lower end of the liquid inlet channel is connected with the lower end of the sample containing cavity.

[0030] Optionally, the body is further provided with an exhaust channel, one end of the exhaust channel is communicated with the sealing cavity, and the other end is communicated with the sample containing cavity.

[0031] Optionally, the lower end of the exhaust channel is connected with the upper end of the sample containing cavity, and the upper end is connected with the sealing cavity.

[0032] Optionally, a liquid injection hole is arranged between the liquid inlet channel and the sealing cavity.

[0033] Optionally, the inner wall of the liquid injection hole is in the structure of a circular truncated cone, the large diameter end of the liquid injection hole is connected with the sealing cavity, and the small diameter end is connected with the liquid inlet channel.

[0034] Optionally, the deformable wall is an aluminum film, a polypropylene film or a polycarbonate film; or the deformable wall comprises an aluminum film and an isolation film, and the isolation film is connected to the inner side of the aluminum film.

[0035] Optionally, the thickness of the deformable wall is not greater than 100 microns.

[0036] Optionally, the thickness of the isolation film is not greater than 50 microns.

[0037] Optionally, an outer surface of the second wall is connected with a reinforcing rib.

[0038] Optionally, the carrier further comprises a reinforcing part, and the reinforcing part is used for reinforcing the body.

[0039] Optionally, the reinforcing part comprises a first reinforcing part, and the first reinforcing part is in a strip structure.

[0040] Optionally, the body is in a cuboid structure, the first reinforcing part is arranged on one side or both sides of the body in a width direction, and a thickness of the first reinforcing part is greater than a thickness of the body.

[0041] Optionally, the reinforcing part further comprises a second reinforcing part, one side or both sides of the body in a length direction are provided with the second reinforcing part, and a thickness of the second reinforcing part is greater than the thickness of the body.

[0042] Optionally, the carrier further comprises a handle part, and the handle part is connected to one end of the body.

[0043] Optionally, the carrier further comprises a positioning part used for positioning the carrier.

[0044] Optionally, the positioning part is a positioning gap arranged in the body or the reinforcing part.

[0045] As can be seen from the above, the technical scheme provided by the present application is that at least part of the sealing element is inserted into the sealing cavity, on the one hand, because the sealing element occupies the space in the body, the gas in the body is compressed, so that the pressure in the body is greater than the external pressure, and then the deformable wall is bulged out. When the temperature adjusting device is arranged on the outside of the deformable wall, because the deformable wall is bulged out, the deformable wall is in close contact with the temperature adjusting device, the heat transfer speed between the deformable wall and the temperature adjusting device is fast, and the temperature rising and falling speed of the reaction sample is fast. If the temperature adjusting device applies sufficient pressure to the carrier to compress the outer surface of the deformable wall into a plane, at this time, the carrier is completely compressed, the temperature adjusting device is in complete contact with the carrier, the fast heat transfer between the temperature adjusting device and the carrier is ensured, and the temperature rising and falling speed and the amplification efficiency of the reaction sample are improved. At the same time, when the outer surface of the deformable wall is compressed into a plane, the volume of the sample containing cavity is reduced relative to the case that the deformable wall is bulged out, so that the gas in the body is further compressed, the air pressure of the sample containing cavity is further increased, and the reaction sample will be in better contact with the deformable wall, which further facilitates the temperature transfer. By reasonably setting the volume of the sample containing cavity, the volume of the sealing cavity and the volume of the sealing element inserted into the sealing cavity, the internal pressure of the sample containing cavity is increased, so that the deformable wall and the temperature adjusting device form good thermal contact, and then the heat conduction efficiency between the deformable wall and the temperature adjusting device is improved, and the amplification efficiency of the reaction sample is improved.

[0046] The carrier provided by the present application does not need to fill gas into the carrier and does not need to introduce more reaction sample into the carrier, but only needs to seal the sealing member in the sealing cavity to adjust the internal pressure of the sample containing cavity, so that the carrier is attached to the temperature adjusting device.

[0047] The reaction sample in the flat structure body or sample containing cavity is thin, the distance between the center of the reaction sample and the surface of the liquid is small, the temperature of the reaction sample can reach uniformity in a short time, and the flat structure can make the contact area of the reaction sample and the temperature adjusting device large and the heat transfer efficiency high, so that the heating and cooling speed of the reaction sample and the detection efficiency are greatly improved. The inner diameter of the tube type carrier is larger than the sample containing cavity of the flat structure, the distance between the center of the reaction sample and the surface of the liquid is large, the temperature of the reaction sample needs a long time to reach uniformity, the heating and cooling speed of the reaction sample is low, and the detection efficiency is low.

[0048] The exhaust passage is arranged on the body, the air in the sample containing cavity can be discharged through the exhaust passage, the residual air in the sample containing cavity is avoided, so that the rapid uniform temperature and the heating and cooling of the reaction sample are ensured.

[0049] The carrier or sample containing cavity is in a flat structure, and the carrier or sample containing cavity is vertically arranged when the reaction sample is amplified, so that the gas generated in the sample containing cavity during the heating process in the amplification stage is more easily floated to the upper end of the body, and will not be dispersed in the sample containing cavity. At the same time, during the amplification process, the carrier is vertically placed, the liquid generates thermal convection, which is beneficial to further mix the liquid and improve the temperature uniformity of the reaction sample. BRIEF DESCRIPTION OF DRAWINGS

[0050] Figure 1a is a structural schematic diagram of the body provided by the embodiment of the present application;

[0051] Figure 1b is a structural schematic diagram of the body provided by the embodiment of the present application when the sealing member is not covered on the body and the body is empty;

[0052] Figure 1c is a structural schematic diagram of the body provided by the embodiment of the present application when the sealing member is not covered on the body and the body is full;

[0053] Figure 1d is a structural schematic diagram of the body provided by the embodiment of the present application when the sealing member is covered on the body and the body is full;

[0054] Figure 1e is a structural schematic diagram of the carrier provided by the embodiment of the present application when the carrier is clamped between two groups of temperature adjusting devices;

[0055] Figure 2a is a structural schematic diagram of another body provided by the embodiment of the present application;

[0056] Figure 2b is a structural schematic diagram of the sealing member not covering another body and the body being empty according to an embodiment of the present application;

[0057] Figure 2c is a structural schematic diagram of the sealing member not covering another body and the body being full according to an embodiment of the present application;

[0058] Figure 2d is a structural schematic diagram of the sealing member covering another body and the body being full according to an embodiment of the present application;

[0059] Figure 2e is a structural schematic diagram of the carrier contacting and pressing the temperature adjusting device according to an embodiment of the present application;

[0060] Figure 3a is an exploded view of the temperature adjusting device according to an embodiment of the present application;

[0061] Figure 3b is a perspective view of the sealing member covering the body and the carrier removing the first wall according to an embodiment of the present application;

[0062] Figure 4 is a perspective view of the sealing member not covering the body and the carrier removing the first wall according to an embodiment of the present application;

[0063] Figure 5 is a sectional view of the carrier when the sealing member does not cover the body according to an embodiment of the present application;

[0064] Figure 6 is Figure 5 is a partial enlarged view of A in FIG. 8;

[0065] Figure 7 is a sectional view of the carrier when the sealing member covers the body according to an embodiment of the present application;

[0066] Figure 8 is Figure 7 is a partial enlarged view of B in FIG. 9;

[0067] Figure 9 is a perspective view of the carrier from another angle when the sealing member covers the body according to an embodiment of the present application;

[0068] Figure 10 is a structural schematic diagram of the temperature adjusting device according to an embodiment of the present application;

[0069] Figure 11 is a structural schematic diagram of the carrier inserted into the temperature adjusting device according to an embodiment of the present application.

[0070] in the figure:

[0071] 1, body; 11, first wall; 12, second wall; 13, side wall; 14, sample containing cavity; 15, sealing cavity; 16, annular wall;

[0072] 2, sealing element; 21, second plug column; 22, sealing part; 221, sealing convex ring; 23, positioning cap; 24, connecting strip;

[0073] 3, liquid inlet channel; 4, exhaust channel; 5, liquid injection hole; 6, reinforcing rib; 7, first reinforcing part; 8, second reinforcing part; 9, positioning part; 10, handle;

[0074] 100, temperature adjusting device; 101, heater; 102, cooler; 103, plug; 104, clamping groove; 105, positioning protrusion;

[0075] 200, fluorescence detection device. DETAILED DESCRIPTION

[0076] The technical solutions of the present application will be further described below in combination with the drawings and through specific embodiments. It can be understood that the specific embodiments described herein are only used to explain the present application, and not to limit the present application. In addition, it should be noted that, for the convenience of description, only the parts related to the present application are shown in the drawings, not all.

[0077] Some orientation words are defined in the present application. Unless otherwise stated and limited, the orientation words such as "up", "down", "left", "right", "in", "out" are used for easy understanding, and thus do not constitute a limitation on the scope of protection of the present application.

[0078] In the present application, unless otherwise explicitly specified and limited, the "upper" or "lower" of the first feature to the second feature can include that the first and second features are in direct contact, or that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, the "above", "over" and "on" of the first feature to the second feature includes that the first feature is directly above and obliquely above the second feature, or only means that the first feature is higher than the second feature in horizontal height. The "below", "under" and "under" of the first feature to the second feature includes that the first feature is directly below and obliquely below the second feature, or only means that the first feature is lower than the second feature in horizontal height.

[0079] In the description of the present application, unless otherwise explicitly specified and limited, the terms "connected", "linked", "fixed" should be understood broadly, for example, can be fixedly connected, or can be detachably connected, or integrated; can be mechanically connected, or electrically connected; can be directly connected, or indirectly connected through an intermediate medium, or the internal communication of two elements or the interaction relationship between two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0080] The embodiment provides a carrier, which can be used for containing a reaction sample to be amplified, but is not limited thereto, and can also be used in other occasions.

[0081] As shown in Figures 1a-1e , the carrier provided by the embodiment comprises a body 1 and a sealing member 2, the body 1 is provided with a sample containing cavity 14 and a sealing cavity 15, the sample containing cavity 14 is in communication with the sealing cavity 15, at least part of the sealing member 2 can be sealingly inserted into the sealing cavity 15, and at least part of the cavity wall of the sample containing cavity 14 is a deformable wall.

[0082] As shown in Figure 1a and 2a , the body 1 comprises a first wall 11 and a second wall 12 arranged oppositely, and a side wall 13 arranged between the first wall 11 and the second wall 12, as shown in Figure 1a , the first wall 11 and the second wall 12 comprise a deformable wall, or as shown in Figure 2a , the first wall 11 comprises a deformable wall.

[0083] As shown in Figure 1c and Figure 2c , when the reaction sample is carried in the sample containing cavity 14 and the sealing member 2 is not inserted into the sealing cavity 15, the pressure in the body 1 is consistent with the external pressure. As shown in Figure 1d and Figure 2d , at least part of the sealing member 2 is sealingly inserted into the sealing cavity 15, on the one hand, because the sealing member 2 occupies the space in the body 1, therefore, the gas in the body 1 is compressed, such as when the sample containing cavity 14 is filled with the reaction sample (as shown in Figure 1c and Figure 2c ), the gas in the sealing cavity 15 is compressed, or when the reaction sample is carried in the sample containing cavity 14, but the reaction sample does not fill the sample containing cavity 14, the gas in the sealing cavity 15 and the sample containing cavity 14 is compressed, so that the pressure in the body 1 is greater than the external pressure (usually atmospheric pressure), and then the deformable wall is protruded outward. As shown in Figure 1e and Figure 2eAs shown, when the temperature adjusting device 100 is attached to the deformable wall by external force, the pressure inside the body 1 is greater than the external pressure, so that the deformable wall is in close contact with the temperature adjusting device 100, the air gap between them is reduced, the heat transfer speed between the deformable wall and the temperature adjusting device 100 is fast, and the heating and cooling speed of the reaction sample is fast. As shown in Figure 3a As shown, it can be understood that the temperature adjusting device 100 can include a heater 101 and / or a cooler 102, such as the heater 101 being in direct contact with the cooler 102, the carrier being in contact with the heater 101, the heater 101 heating the reaction sample, and the cooler 102 cooling the heater 101, thereby cooling the reaction sample, and further realizing PCR amplification. The cooler 102 can also be connected to the plug-in block 103, and the plug-in block 103 is provided with a clamping groove, and the carrier can be inserted into the clamping groove, thereby limiting the carrier.

[0084] As shown in Figure 1e and Figure 2e Further, due to the pressure inside the body 1 being greater than the external pressure, the deformable wall will slightly protrude outward, and if the temperature adjusting device 100 applies sufficient pressure to the carrier to press the outer surface of the deformable wall into a plane, the carrier is completely pressed, the temperature adjusting device 100 is in complete contact with the carrier, ensuring fast heat transfer between the temperature adjusting device 100 and the carrier, and improving the heating and cooling speed of the reaction sample and the amplification efficiency. At the same time, when the outer surface of the deformable wall is pressed into a plane, the volume of the sample containing cavity 14 is reduced compared to when the deformable wall protrudes outward, so that the gas inside the body 1 is further compressed, and the gas pressure in the sample containing cavity 14 is further increased compared to Figure 1d and Figure 2d , the temperature adjusting device 100 will be in better contact with the deformable wall, further facilitating temperature transfer.

[0085] As shown in Figure 1b and Figure 2b , for example, the volume of the sample containing cavity 14 is designed as V1, the volume of the sealing cavity 15 is V2, and the volume of the sealing member 2 inserted into the sealing cavity 15 is V3; as shown in Figure 1c and Figure 2c , after a fixed volume V1 of reaction sample is added to the sample containing cavity 14, the reaction sample fills the sample containing cavity 14; after the sealing member 2 is sealed on the sealing cavity 15 (as shown in Figure 1d and Figure 2d ), the sealing member 2 compresses the gas with a volume of V3, so that the deformable wall of the sample containing cavity 14 bulges, and at this time the liquid level of the reaction sample must be lowered; as shown in Figure 1e and Figure 2e , after the temperature adjusting device 100 and the carrier are completely pressed by applying sufficient pressure, the reaction sample will completely fill the sample containing cavity 14 again, at this time the gas is further compressed, and the gas pressure in the sample containing cavity 14 is further increased compared to Figure 1dand Figure 2d Further increase, temperature regulating device 100 will be in better contact with the deformable wall, conducive to temperature transfer. The carrier provided by the present application does not need to be additionally filled with gas into it and does not need to be more reaction sample into the carrier, but only needs to seal the seal in the sealed cavity to adjust the internal pressure of the sample containing cavity 14, so that the carrier is attached to the temperature regulating device 100.

[0086] It can be understood that the seal 2 can be completely inserted into the sealed cavity 15, or partially inserted into the sealed cavity 15, and in addition, the sealed cavity 15 can be completely occupied by the seal 2, or not completely occupied (such as Figure 7 and Figure 8 It is shown). The volume of the sealed cavity 15 and the seal 2 can be reasonably designed, and the design principle is that after the seal 2 is sealed and inserted into the sealed cavity 15, the temperature regulating device 100 flattens the deformable wall (at this time, if the filling amount of the reaction sample is consistent with the containing amount of the sample containing cavity 14, the reaction sample can completely fill the sample containing cavity 14), The sample containing cavity 14 forms a positive pressure with the outside (usually atmospheric pressure), for example: the pressure difference between the sample containing cavity 14 and the outside is 0.5 bar, 1 bar, 1.5 bar, etc., but the pressure difference is not limited to this.

[0087] That is, the present embodiment reasonably sets the volume of the sample containing cavity 14, the volume of the sealed cavity 15, and the volume of the seal 2 inserted into the sealed cavity 15, so that the internal pressure of the sample containing cavity 14 is increased, thereby forming good thermal contact between the deformable wall and the temperature regulating device 100, and further improving the heat conduction efficiency between the deformable wall and the temperature regulating device 100, and improving the amplification efficiency of the reaction sample.

[0088] In an alternative embodiment, while ensuring the strength of the deformable wall, to further improve the heat conduction speed between the deformable wall and the temperature regulating device 100, the deformable wall is a deformable film. For example, the thickness of the deformable wall is not more than 100 microns.

[0089] The deformable wall can be an aluminum film, a polypropylene film, or a polycarbonate film; or the deformable wall includes an aluminum film and an isolation film, the isolation film is connected to the inner side of the aluminum film, and the isolation film directly contacts the reaction sample to separate the aluminum film and the reaction sample, thereby avoiding the influence of the aluminum film on the reaction sample. At this time, the thickness of the deformable wall is not more than 100 microns, which can have sufficient strength. The isolation film can be a polypropylene film or a polycarbonate film. The thickness of the isolation film is not more than 50 microns, such as not more than 30 microns, specifically, such as 30 microns, 25 microns, 20 microns, etc. It can be understood that the smaller the thickness of the isolation film, the better, which is conducive to rapid heat conduction between the temperature regulating device 100 and the reaction sample, and reduces the lag time of the reaction sample temperature relative to the temperature of the temperature regulating device 100.

[0090] Optionally, the body 1 and / or the sample containing cavity 14 is flat. It can be understood that the flat structure can mean that the size of the thickness direction (the direction in which the first wall 11 and the second wall 12 are arranged is the thickness direction (Y) of the carrier) of the body 1 or the sample containing cavity 14 is smaller than the size of the width direction (X) or the length direction (Z), for example, the ratio of the size of the sample containing cavity 14 or the body 1 perpendicular to the thickness direction (Y) to the size of the thickness direction (Y) is greater than 5:1, such as the size ratio of 50:1 to 100:1.

[0091] For example, the sample containing cavity 14 is a cuboid, and the ratio of the length and thickness of the cuboid can be greater than 5:1, such as the size ratio of 50:1 to 100:1, for example, the size ratio is 90:1, such as the thickness of the sample containing cavity 14 can be 0.3-1.0mm, and the width and length of the sample containing cavity 14 are about 10mm and 20mm respectively. For example, the sample containing cavity 14 can also be a cylindrical structure, and the ratio of the diameter and thickness is greater than 5:1, such as the thickness is 0.3-1.0mm, and the diameter is 5-20mm. Of course, the cross section of the sample containing cavity 14 can be polygonal or elliptical, etc. Of course, the cross section of the sample containing cavity 14 can be polygonal or elliptical, etc.

[0092] The reaction sample in the flat body 1 or the sample containing cavity 14 is thin, the center of the reaction sample is far away from the surface of the liquid, the temperature of the reaction sample can reach uniformity in a very short time, and the flat structure can make the contact area of the reaction sample with the temperature adjusting device 100 large, the heat transfer efficiency high, so that the heating and cooling speed of the reaction sample and the detection efficiency are greatly improved. The inner diameter of the tubular carrier is larger than the size of the sample containing cavity 14 of the flat structure, the center of the reaction sample is far away from the surface of the liquid, and the temperature of the reaction sample needs a long time to reach uniformity, so that the heating and cooling speed of the reaction sample is low and the detection efficiency is low.

[0093] In addition, as shown in Figure 1e and Figure 2e , when the reaction sample is amplified, the flat carrier or the sample containing cavity is arranged vertically, that is, as shown in Figure 1e and Figure 2e , the thickness direction (Y) of the carrier is perpendicular to the vertical direction (Z), so that the gas generated in the heating process of the reaction sample in the sample containing cavity 14 is more easily floated to the upper end of the body 1, and will not be dispersed everywhere in the sample containing cavity 14, and at the same time, during the amplification process, the carrier is placed vertically, the liquid generates thermal convection, which is beneficial to further mix the liquid and improve the temperature uniformity of the reaction sample.

[0094] As shown in Figures 1a-1eAs shown, the first wall 11 and the second wall 12 are deformable walls at least at positions opposite to the sample containing cavity 14, and the temperature adjusting device 100 heats and cools on both sides of the carrier (i.e. the first wall 11 and the second wall 12), which further improves the temperature rising and falling speed of the reaction sample. Further, the deformable wall can be sealed to the side wall 13 by means of hot pressing, laser welding or ultrasonic welding.

[0095] At this time, the side wall 13 is made of transparent material, and the fluorescence detection device 200 can detect the fluorescence of the reaction sample through the transparent side wall 13. In this embodiment, the side wall 13 is set as a transparent wall, so that the reaction sample can be directly detected after amplification without transferring the carrier and the reaction sample, thereby improving the detection efficiency.

[0096] As shown in Figures 2a-2e , the first wall 11 (or the second wall 12) is a deformable wall at least at positions opposite to the sample containing cavity 14, i.e. the first wall 11 of the carrier is deformable, and the second wall 12 is not deformable or has a very small deformation degree compared with the first wall 11. Preferably, the thickness of the second wall 12 is not greater than 0.5 mm, so as to reduce the heat absorption of the second wall 12 during temperature rising and falling, thereby reducing the time for the reaction sample to reach temperature equilibrium and accelerating the temperature rising and falling speed of the reaction sample.

[0097] At this time, the second wall 12 and / or the side wall 13 is made of transparent material, and the fluorescence of the reaction sample can be detected through the second wall 12 or the side wall 13. When the fluorescence is detected through the second wall 12, the temperature adjusting device 100, the carrier and the fluorescence detection device 200 can be sequentially arranged, so that the compactness of the equipment is better and the equipment occupies less space.

[0098] As shown in Figures 2a-2e , the second wall 12 and the side wall 13 can also be integrally formed by injection molding, so as to improve the processing efficiency of the carrier. Further, the deformable wall can be sealed to the side wall 13 by means of hot pressing, laser welding or ultrasonic welding.

[0099] The transparent material can be dimethylsiloxane (i.e. PDMS), polypropylene (i.e. PP), organic glass (i.e. PMMA) or polycarbonate (i.e. PC), and PMMA, PDMS, PP and PC are optical transparent materials with good biocompatibility, so they can meet the fluorescence detection requirements and have no effect on the reaction sample.

[0100] As shown in Figure 1b and Figure 2bAs shown, the main body 1 may also have a liquid inlet channel 3. The sample receiving cavity 14 and the sealing cavity 15 are connected through the liquid inlet channel 3, and liquid is injected and vented through the sealing cavity 15 and the liquid inlet channel 3. At this time, when the sealing element 2 is inserted into the sealing cavity 15, the air in the sealing cavity 15 and the liquid inlet channel 3 is compressed. The cross-sectional area of ​​the liquid inlet channel 3 is preferably smaller than the cross-sectional area of ​​the sealing cavity 15, thereby increasing the pressure difference between the inside of the main body 1 and the outside.

[0101] like Figure 3b As shown, when only the first wall 11 is a deformable wall, the sample receiving cavity 14 and the liquid inlet channel 3 can be formed by slotting the second wall 12 and then covering the first wall 11 with the slotted side of the second wall 12, such as by injection molding to form the groove or by machining.

[0102] like Figure 3b and Figure 4 As shown, when the reaction sample is amplified, the carrier is placed vertically, the sealing cavity 15 is located at the upper end of the carrier, the upper end of the liquid inlet channel 3 is connected to the sealing cavity 15, and the lower end of the liquid inlet channel 3 is connected to the lower end of the sample receiving cavity 14. Therefore, when liquid is injected into the sample receiving cavity 14, the reaction sample enters from the lower end of the sample receiving cavity 14, and the air is located above the reaction sample. The air floats up and is easier to expel, such as through the liquid inlet channel 3.

[0103] Furthermore, since the carrier is relatively thin, the size of the liquid inlet channel 3 is also relatively small. To avoid affecting the air discharge from the liquid inlet channel 3 during the liquid inlet process, optionally, an exhaust channel 4 is also provided on the main body 1. One end of the exhaust channel 4 is connected to the sealing cavity 15, and the other end is connected to the sample receiving cavity 14. At this time, when liquid is introduced into the liquid inlet channel 3, the air in the sample receiving cavity 14 can be discharged through the exhaust channel 4, avoiding residual air in the sample receiving cavity 14, thereby ensuring rapid and uniform temperature rise and fall of the reaction sample.

[0104] To ensure complete air expulsion, the lower end of the exhaust channel 4 is connected to the upper end of the sample receiving cavity 14, and the upper end is connected to the sealing cavity 15. During liquid injection, air rises and enters the lower end of the exhaust channel 4, and is then discharged through the exhaust channel 4. Meanwhile, the carrier is placed vertically, and the lower end of the exhaust channel 4 will not be blocked by the reaction solution until the sample receiving cavity 14 is filled with the reaction sample, allowing the exhaust channel 4 to continuously ventilate.

[0105] like Figure 4 and Figure 5 As shown, an injection hole 5 is provided between the liquid inlet channel 3 and the sealing cavity 15. Pipette tips and other pipetting tools can be inserted into the injection hole 5 to inject liquid into the sample receiving cavity 14.

[0106] like Figure 5As shown, optionally, the inner wall of the liquid injection hole 5 is in a circular truncated cone structure, the large diameter end of the liquid injection hole 5 is connected with the sealing cavity 15, and the small diameter end is connected with the liquid inlet channel 3, so as to adapt to the liquid transfer operation of the liquid transfer gun head.

[0107] It can be understood that when the carrier is provided with the exhaust channel 4, the liquid inlet channel 3, the liquid injection hole 5, and no reaction sample is carried therein, the sealing element 2 is sealingly inserted into the sealing cavity 15, and the air in the exhaust channel 4, the liquid inlet channel 3, the liquid injection hole 5, and the sealing cavity 15 is compressed.

[0108] Optionally, the sealing element 2 is in interference fit with the sealing cavity 15, which can seal the sealing cavity 15 on the one hand, and can avoid the sealing element 2 from being pushed open when there is a pressure difference between the body 1 and the outside on the other hand. Of course, in other optional embodiments, the sealing element 2 and the sealing cavity 15 can also be sealingly connected by other ways.

[0109] Illustratively, the sealing element 2 includes a first plug column (not shown in the figure), and the first plug column is sealingly arranged in the sealing cavity 15. At this time, the first plug column is in interference fit with the sample containing cavity 14, and the volume of the first plug column inserted into the sealing cavity 15 is the volume of the air compression in the body 1. Optionally, the first plug column is made of elastic materials such as rubber or silicone.

[0110] However, when the sealing element 2 is in the above structure, since the first plug column is in interference fit with the sample containing cavity 14, the operator needs to exert a relatively large force to insert the first plug column into the sealing cavity 15.

[0111] To solve the above technical problems, as shown in the figure, Figures 5-8 The sealing element 2 includes a second plug column 21 and a sealing part 22 connected with each other, the sealing part 22 is arranged around the outer periphery of the second plug column 21 and is arranged in space with the outer periphery of the second plug column 21, and the outer periphery of the sealing part 22 can be sealingly contacted with the cavity wall of the sealing cavity 15. At this time, the second plug column 21 and the sealing part 22 are both inserted into the sealing cavity 15, and the volume of the second plug column 21 and the sealing part 22 inserted into the sealing cavity 15 is the volume of the air compression in the body 1.

[0112] The sealing part 22 can be in interference fit with the sealing cavity 15, when the sealing element 2 is inserted into the sealing cavity 15, the sealing part 22 is subjected to the force of the cavity wall of the sealing cavity 15, so that the sealing cavity 15 moves towards the second plug column 21, reduces the friction between the cavity wall of the sealing cavity 15 and the sealing part 22, and then facilitates the insertion of the sealing element 2 into the sealing cavity 15. Optionally, the sealing part 22 is made of elastic materials such as rubber or silicone.

[0113] As shown in the figure, Figure 6 and Figure 8As shown, to further reduce the friction between the cavity wall of the sealing cavity 15 and the sealing part 22, a sealing protrusion 221 is formed on the outer periphery of the sealing part 22, and the sealing protrusion 221 makes sealing contact with the cavity wall of the sealing cavity 15. The sealing protrusion 221 can seal the sealing cavity 15, and at the same time, it can reduce the contact area between the sealing part 22 and the cavity wall of the sealing cavity 15, thereby reducing the friction between the cavity wall of the sealing cavity 15 and the sealing part 22.

[0114] In this embodiment, the volume of the seal 2 inserted into the sealing cavity 15 determines the pressure difference between the inside and outside of the carrier. To improve the accuracy of the depth of the seal 2 inserted into the sealing cavity 15, the seal 2 may optionally include a positioning cap 23. The ends of the second plug 21 and the sealing part 22 are both connected to the positioning cap 23, which can abut against the outer surface of the body 1. That is, the positioning cap 23 plays a positioning role, and when the positioning cap 23 abuts against the outer surface of the body 1, the seal 2 is inserted into place.

[0115] like Figure 4 and Figure 5 As shown, in this embodiment, the carrier has a small thickness. To facilitate the formation of the sealed cavity 15 and reduce the heat absorption of the carrier wall, thereby improving the sample's temperature homogenization rate, the body 1, exemplarily, also includes an annular wall 16 connected to the side wall 13. The space within the annular wall 16 forms the sealed cavity 15. Further, the annular wall 16 is a circular ring structure, the cross-section of the sealed cavity 15 is a cylindrical structure, the first plug is a cylindrical structure, and the cross-section of the sealing part 22 is annular, thus sealing the sealed cavity 15. Of course, it can be understood that the annular wall 16 can also be a square ring or other polygonal ring, as long as the annular wall 16 extends continuously and forms a circumferentially closed structure.

[0116] To prevent the seal 2 from being lost, the seal 2 can be connected to the body 1 by the connecting strip 24.

[0117] Optionally, the seal 2 and the body 1 can be integrally molded to facilitate the processing of the carrier.

[0118] In this embodiment, both the first wall 11 and the second wall 12 are relatively thin to improve the thermal conductivity between the reaction sample and the temperature control device 100, and to reduce heat loss caused by heat conducted to the first wall 11 and the second wall 12. Therefore, in order to satisfy both the rapid heating and cooling of the reaction sample and the requirement that the carrier has sufficient strength, such as... Figure 9 As shown, the carrier may optionally include a reinforcing part for reinforcing the body 1.

[0119] Specifically, the reinforcing portion comprises a first reinforcing portion 7, which can be in a strip structure to improve the strength of the carrier and avoid distortion of the carrier. Meanwhile, the first reinforcing portion 7 is away from the sample accommodation cavity and does not affect the rapid temperature change of the reaction sample and the detection of the reaction sample. The thickness of the first reinforcing portion 7 is greater than the thickness of the body 1 (i.e., the size of the first reinforcing portion 7 in the thickness direction (Y) is greater than the size of the body 1 in the thickness direction (Y)) to improve the strength of the carrier and avoid distortion of the carrier. For example, the body 1 is in a cuboid structure, and the first reinforcing portion 7 is arranged on one side or both sides of the body 1 in the width direction (X). For example, the width direction (X), the thickness direction (Y), and the vertical direction (Z) are perpendicular to each other.

[0120] The reinforcing portion further comprises a second reinforcing portion 8 arranged on one side or both sides of the body 1 in the length direction (i.e., the vertical direction (Z)). The thickness of the second reinforcing portion 8 is greater than the thickness of the body 1 to improve the strength of the carrier and avoid distortion of the carrier. Specifically, the second reinforcing portion 8 is arranged at the upper end of the body 1. Optionally, the second reinforcing portion 8 is located between the two first reinforcing portions 7. Further, the second reinforcing portion 8 is arranged close to the upper end of the two first reinforcing portions 7.

[0121] As shown in Figure 9 , when only the first wall 11 is a variable wall, the outer surface of the second wall 12 can be connected to the reinforcing rib 6, so that the carrier can be reinforced to avoid distortion such as twisting and to reduce expansion caused by pressure difference.

[0122] In order to insert the carrier between the two temperature adjusting devices 100 (as shown in Figure 1e ) or between the temperature adjusting device 100 and the fluorescence detection device 200 (as shown in Figure 2e ), the carrier further comprises a handle 10 portion connected to one end of the body 1. Optionally, the handle 10 is in a strip structure.

[0123] In order to determine whether the carrier is inserted into the preset position between the two temperature adjusting devices 100, the carrier further comprises a positioning portion 9 for positioning the carrier. Specifically, the positioning portion 9 is a positioning gap arranged on the body 1 or the reinforcing portion. For example, the positioning gap is arranged on the first reinforcing portion 7. As shown in Figure 3a , Figure 10 and Figure 11 , a positioning protrusion 105 can be arranged on the temperature adjusting device 100, such as being connected to the clamping groove 104. When the positioning protrusion 105 enters the positioning gap, it indicates that the carrier is inserted into the preset position.

[0124] The carrier provided by the embodiment enables the temperature adjusting device 100 to quickly transfer heat to the reaction sample in the sample containing cavity 14 through the deformable film; by reasonably designing the pressure inside the carrier, the internal air pressure is large, the deformable film can form good thermal contact with the temperature adjusting device 100, and the air gap between the deformable film and the temperature adjusting device 100 is avoided to affect heat conduction; the sample containing cavity 14 adopts a flat structure design, and the reaction sample temperature can quickly reach equilibrium; through the above technical means, the reaction sample temperature can quickly respond, the lag time of the reaction sample temperature and the temperature adjusting device 100 is reduced, and thus the PCR reaction time is reduced.

[0125] Although the present application has been described in detail with general description, specific embodiments and experiments, some modifications or improvements can be made on the basis of the present application, which is obvious to those skilled in the art. Therefore, these modifications or improvements made on the basis of not deviating from the spirit of the present application, all belong to the scope of the present application.

Claims

1. A vector, characterized in that, The device comprises a body (1) and a sealing member (2), the body (1) is provided with a sample accommodating cavity (14) and a sealing cavity (15), the sample accommodating cavity (14) is communicated with the sealing cavity (15), at least part of the cavity wall of the sample accommodating cavity (14) is a deformable wall, at least part of the sealing member (2) can be sealingly inserted into the sealing cavity (15) to at least compress the gas in the sealing cavity (15) and make the deformable wall protrude outward to tightly contact with a temperature adjusting device (100). The sample accommodating cavity (14) is in a strip shape. The body (1) is further provided with a liquid inlet channel (3), the sample accommodating cavity (14) and the sealing cavity (15) are communicated through the liquid inlet channel (3), the liquid inlet channel (3) is located at the side of the sample accommodating cavity (14) and the width of the liquid inlet channel (3) is smaller than that of the sample accommodating cavity (14), the upper end of the liquid inlet channel (3) is connected with the sealing cavity (15), the lower end of the liquid inlet channel (3) is connected with the lower end of the sample accommodating cavity (14), and the lower end of the liquid inlet channel (3) is in a circular arc shape. The body (1) and / or the sample accommodating cavity (14) is in a flat structure. The body (1) is further provided with an exhaust channel (4), one end of the exhaust channel (4) is communicated with the sealing cavity (15) and the other end is communicated with the sample accommodating cavity (14), and the thickness of the deformable wall is not greater than 100 microns.

2. The carrier of claim 1, wherein, The deformable wall is a deformable membrane.

3. The carrier of claim 1, wherein, The sealing member (2) can be sealingly inserted into the sealing cavity (15) to compress the gas in the sealing cavity (15) and the sample accommodating cavity (14).

4. The carrier of claim 1, wherein, The body (1) comprises oppositely arranged first and second walls (11, 12) and a side wall (13) arranged between the first and second walls (11, 12), and the first wall (11) and / or the second wall (12) comprises the deformable wall.

5. The carrier of claim 4, wherein, The first wall (11) and the second wall (12) are the deformable wall at least at the positions opposite to the sample accommodating cavity (14).

6. The carrier of claim 5, wherein, The side wall (13) is made of a transparent material.

7. The carrier of claim 4, wherein, The first wall (11) is the deformable wall at least at the positions opposite to the sample accommodating cavity (14).

8. The carrier of claim 7, wherein, The second wall (12) and / or the side wall (13) is made of a transparent material.

9. The carrier of claim 7, wherein, The second wall (12) and the side wall (13) are integrally formed.

10. The carrier of claim 7, wherein, The thickness of the second wall (12) is not greater than 0.5 mm.

11. The vector of any one of claims 1-3, wherein, The sealing member (2) comprises a first plug column, and the first plug column is sealingly arranged in the sealing cavity (15).

12. The carrier of claim 1, wherein, The sealing member (2) comprises a second plug column (21) and a sealing part (22) connected with each other, the sealing part (22) is arranged around the outer periphery of the second plug column (21) and is spaced apart from the outer periphery of the second plug column (21), and the outer periphery of the sealing part (22) can sealingly contact with the cavity wall of the sealing cavity (15).

13. The carrier of claim 12, wherein, The sealing part (22) is provided with a sealing convex ring (221) on the outer periphery, which is in sealing contact with the cavity wall of the sealing cavity (15).

14. The carrier of claim 12 or 13, wherein, The sealing part (2) further comprises a positioning cap (23), the second plug column (21) and the end of the sealing part (22) are connected to the positioning cap (23), and the positioning cap (23) can abut against the outer surface of the body (1).

15. The carrier of claim 1, wherein, The sealing part (2) is integrally formed with the body (1).

16. The carrier of claim 4, wherein, The body (1) further comprises an annular wall (16), which is connected to the side wall (13), and the space in the annular wall (16) forms the sealing cavity (15).

17. The carrier of claim 1, wherein, The sealing part (2) is in interference fit with the sealing cavity (15).

18. The carrier of claim 1, wherein, The flat structure refers to that the size of the sample containing cavity (14) or the body (1) perpendicular to the thickness direction is greater than the size in the thickness direction.

19. The carrier of claim 18, wherein, The ratio of the size of the sample containing cavity (14) or the body (1) perpendicular to the thickness direction to the size in the thickness direction is greater than 5:

1.

20. The carrier of claim 18, wherein, The ratio of the size is 50:1~100:

1.

21. The carrier of claim 1, wherein, The lower end of the exhaust channel (4) is connected with the upper end of the sample containing cavity (14), and the upper end is connected with the sealing cavity (15).

22. The carrier of claim 1, wherein, The liquid inlet channel (3) and the sealing cavity (15) are provided with a liquid injection hole (5).

23. The carrier of claim 22, wherein, The inner wall of the liquid injection hole (5) is a circular truncated cone structure, the large diameter end of the liquid injection hole (5) is connected with the sealing cavity (15), and the small diameter end is connected with the liquid inlet channel (3).

24. The vector of any one of claims 1-3, wherein, The deformable wall is an aluminum film, a polypropylene film or a polycarbonate film; or the deformable wall comprises an aluminum film and an isolation film, and the isolation film is connected to the inner side of the aluminum film.

25. The carrier of claim 24, wherein, The thickness of the isolation film is not greater than 50 microns.

26. The carrier of claim 7, wherein, The outer surface of the second wall (12) is connected with a reinforcing rib (6).

27. The carrier of claim 1, wherein, The carrier further comprises a reinforcing part for reinforcing the body (1).

28. The carrier of claim 27, wherein, The reinforcing part comprises a first reinforcing part (7), which is a strip structure.

29. The carrier of claim 28, wherein, The body (1) is a cuboid structure, and the first reinforcing part (7) is arranged on one side or both sides of the body (1) in the width direction, and the thickness of the first reinforcing part (7) is greater than the thickness of the body (1).

30. The carrier of claim 29, wherein, The reinforcing part further comprises a second reinforcing part (8), and the second reinforcing part (8) is arranged on one side or both sides of the body (1) in the length direction, and the thickness of the second reinforcing part (8) is greater than the thickness of the body (1).

31. The vector of any one of claims 1-3, wherein, The carrier further comprises a handle part (10), which is connected to one end of the body (1).

32. The vector of claim 1 or 27, wherein The carrier further comprises a positioning part (9) for positioning the carrier.

33. The carrier of claim 32, wherein, The positioning part (9) is a positioning notch opened in the body (1) or the reinforcing part.

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