Method for preventing and treating resistant aphids by jointly applying orius sativus and afidopyropen

Abstract

The invention relates to the field of biological and chemical prevention and control, in particular to a method for preventing and controlling aphids by combining predatory natural enemies orius sativus and afidopyropen, and the method comprises the step of preventing and controlling the aphids with resistance to chemical pesticides. The invention provides a novel prevention and control technology combining biological prevention and control and chemical prevention and control, aims at combining'insect control by insects' and'insect control by medicines', and explores a novel prevention and control way. The predatory natural enemy can prey for prevention and control for a long time, the unique action mechanism and combined prevention and control of the afidopyropen are exerted, the prevention and control effect is remarkably improved, the duration time is long, the cost is saved by 80% or above compared with that of independent application of the predatory natural enemy, and the prevention and control effect is better than that of independent application of the afidopyropen. The combined application of natural enemies and pesticides can reduce the use amount of chemical pesticides, reduce the damage rate of crops, delay the generation of pest resistance to drugs, develop a new means of pesticides in the field of pest control, and open up an important way for a comprehensive control technical system of resistant aphids.

Description

Technical Field

[0001] The invention belongs to the technical field of agricultural pest control, and particularly relates to a method for controlling resistant aphids by utilizing a predatory natural enemy, the oriental flower stink bug, in combination with diprofiloconazole. Background Art

[0002] The East Asian flower bug, Orius sauteri, belongs to the genus Orius in the family Anthocoridae of the order Hemiptera. It is an important natural enemy resource in my country. Both its adults and nymphs can prey on small pests such as aphids, thrips, spider mites, as well as eggs and young larvae of various Lepidoptera pests. The East Asian flower bug has strong adaptability, a wide distribution range, and a large number of occurrences in nature. It is a small predatory natural enemy with high utilization value and great potential for biological control. It is reported that releasing the East Asian flower bug in the greenhouse pepper greenhouse has a control effect of more than 90% on cinnabarin spider mites, thrips, and aphids; the East Asian flower bug has a release density of 2 heads / m 2 When the control effect of thrips in the peak period of greenhouse eggplant reached 94.46%, the East Asian flower stink bug, as an important natural enemy insect in the farmland ecosystem, has a very high application value.

[0003] Abiprofen is a new type of biogenic insecticide with a different mechanism of action from conventional insecticides. It interferes with the insect vanillic acid transient channel receptor complex, causing the insect to lose its motor coordination and sense of direction, and lose its body sensitivity to gravity, balance, sound, position and movement, which in turn causes the insect to be unable to feed and eventually die of starvation. Abiprofen is highly toxic to target pests, but has low toxicity or even no toxicity to most non-target organisms. For example, abiprofen has a good control effect on piercing-sucking mouthparts pests, such as aphids, whiteflies, planthoppers, psyllids, scale insects and leafhoppers, and has the advantages of high efficiency, long lasting effect, low toxicity and short safe interval. In addition, abiprofen has low toxicity to natural enemies such as small flower bugs, predatory mites, lacewings and ladybugs.

[0004] Aphids are a general term for the superfamily Aphidoidea of ​​the order Hemiptera. There are a total of 10 families and about 4,400 species of aphids that have been discovered. Aphids like to live in groups, often gathering on seedlings, young leaves, tender stems and leaves near the ground to feed on host sap. They can reproduce 20-30 generations a year, and can give birth to offspring in just 5 days after birth; their lifespan is about 3 months. When aphids cause harm, they will excrete a large amount of honeydew to contaminate leaves and fruits, thereby causing sooty mold and affecting plant photosynthesis. Aphids can also spread viral diseases, causing large-scale outbreaks of viral diseases. Insecticides are the main method of prevention and control, but aphids have developed significant resistance to many commonly used insecticides, including organophosphates, carbamates, pyrethroids and neonicotinoids. In recent years, some progress has been made in the use of natural enemies to control aphids. Under the current situation of frequent pest outbreaks, one prevention and control measure is often difficult to solve all problems, and there is an urgent need to vigorously develop an integrated pest management strategy.

[0005] The present invention combines biological control with chemical control technology. First, by evaluating the toxicity of cyclohexanone to target pests and the safety of natural enemies, the feasibility of predatory natural enemies and cyclohexanone combined to prevent and control resistant aphids is determined, and the technical method of combined application is improved. The combined application technology of the present invention can not only play the function of predatory natural enemies to prey on pests, but also use cyclohexanone to control pests to prevent their rapid growth in number, save manpower and material resources, expand the population of natural enemies in the field, reduce the amount of chemical pesticides used, delay the generation of drug resistance, and play a good control effect. Combining predatory natural enemies with cyclohexanone to control pests and play the combined effect of the two has a very broad application prospect. Summary of the invention

[0006] The purpose of the present invention is to provide a method for controlling resistant aphids by combining a predatory natural enemy with a pesticide. The present invention utilizes a combination of a predatory natural enemy, the quintosporin, and the diprofenopterin to give full play to their respective advantages and improve the scope and efficacy of controlling harmful organisms.

[0007] The method for controlling pests by using the predatory natural enemy Ornithogalum spp. in combination with diprofiloxan according to the present invention comprises the following steps:

[0008] (1) First, the toxicity of diprofil to the oriental flower bug and resistant aphids was preliminarily evaluated;

[0009] (2) Based on the aphid's resistance to pesticides, the pesticide concentration is roughly determined, which is generally between the aphid's LC 20 The control effect can be achieved between the recommended dose and the recommended dose;

[0010] (3) spraying diprofil solution;

[0011] (4) Releasing adult East Asian flower stink bugs with a benefit-to-harm ratio higher than 1:300 can achieve control effectiveness;

[0012] The present invention can be used to control aphids such as cotton aphid, peach aphid, apple aphid, vegetable aphid, soybean aphid, corn aphid, etc., which have developed resistance, and various small piercing-sucking pests such as whiteflies, planthoppers, psyllids, scale insects and leafhoppers according to different concentrations of cyproconazole and the feeding habits of the East Asian flower stink bug. Another object of the present invention is to provide the application of the method in improving the effect of the predatory natural enemy East Asian flower stink bug in crops in controlling aphid pests. The present invention combines the predatory natural enemy East Asian flower stink bug and cyproconazole to control aphids, so as to achieve the purpose of reducing the amount of pesticide used and delaying the generation of resistance. When the present invention is used, the cost is saved by more than 80% compared with the use of predatory natural enemies alone, and the control effect is better than the use of cyproconazole alone. The technology of the present invention has a good effect in reducing the damage rate of crops, improving the quality of my country's export crops, and protecting biodiversity and natural enemy resources, and has opened up a new means in the field of chemical pesticide control of pests, and has opened up an important way for the comprehensive control technology system of aphid pests. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 The dynamic changes of resistant cotton aphid population under each treatment. DETAILED DESCRIPTION

[0014] We first used the immersion method to determine the direct toxicity of different concentrations of dimethoate to the fifth-instar nymphs and adults of the East Asian flower bug. 72 hours after treatment, the number of deaths in each treatment was counted, and the slope, chi-square value, and sublethal concentration of the toxicity regression equation of the East Asian flower bug were calculated using PoloPlus 2.0 software. 50 It was determined that diprofil is very safe against the East Asian small flower stink bug at all developmental stages.

[0015] Table 1 Direct toxicity of diprofil to Orientia spp.

[0016]

[0017] In order to make the objects, schemes and advantages of the present invention more clear, the present invention is further described in detail below in conjunction with specific experiments.

[0018] Example 1: Control

[0019] Take 8 pots of cotton plants planted indoors, label them, cover all the cotton plants with insect-proof nets, and inoculate 5 resistant wingless adult aphids on each plant for 3 days. Spray 80 ml of clean water evenly, and count the number of live aphids every 2 days after the test until the 21st day. Repeat the test 3 times, and use the results of this test as a control.

[0020] Example 2: Using Orientia spp. alone

[0021] Take 8 pots of cotton plants planted indoors, label them respectively, cover all the cotton plants with insect-proof nets, and inoculate 5 resistant wingless adult aphids on each plant for 3 days. Randomly inoculate 2 East Asian flower stink bugs on the 8 cotton plants (benefit-harm ratio is about 1:120), and count the number of live aphids every 2 days after the test until the 21st day. The test was repeated 3 times, and the East Asian flower stink bugs released each time must be of the same age and the individuals must not differ much.

[0022] Example 3: Use of the maximum recommended field dose of abicyclon alone

[0023] Take 8 pots of cotton plants planted indoors, label them, cover all the cotton plants with insect-proof nets, and inoculate 5 resistant wingless adult aphids on each plant for 3 days. Spray 80 ml of 16.00 mg / L (maximum recommended field dose) of cyclohexidine solution evenly, and count the number of live aphids every 2 days after the test until the 21st day. Repeat the test 3 times.

[0024] Example 4: Use of low dose of cyclohexanil alone

[0025] Take 8 pots of cotton plants grown indoors, label them, cover all the cotton plants with insect-proof nets, and inoculate 5 resistant wingless adult aphids on each plant for 3 days. Spray 80 ml of 3.49 mg / L (aphid sublethal dose LC 30 ) diprofil solution, and the number of live aphids was counted every 2 days until the 21st day. The experiment was repeated 3 times.

[0026] Example 5: Combined application of low-dose diprofil and Ornithopterus fasciatus

[0027] Take 8 pots of cotton plants grown indoors, label them, cover all the cotton plants with insect-proof nets, and inoculate 5 resistant wingless adult aphids on each plant for 3 days. Spray 80 ml of 3.49 mg / L (aphid sublethal dose LC 30 ) dimethoate solution, and then randomly inoculated 2 East Asian flower bugs on 8 pots of cotton plants (benefit-harm ratio is about 1:120), and counted the number of live aphids every 2 days until the 21st day. The experiment was repeated 3 times, and the East Asian flower bugs released each time must be of the same age and the individuals must not differ much.

[0028] Table 2 The control effect of combined application of cypermethrin and oriental flower bug on resistant cotton aphid

[0029]

[0030] Note: The data in the table are composed of mean ± standard error; different lowercase letters (a, b, c, d) indicate significant differences among the treatment groups (P<0.05).

[0031] The test results showed that the LC values ​​of dimethoate against the fifth instar nymphs, female adults and male adults of the oriental flower bug were 50 The values ​​of 592.38, 497.02 and 382.71 mg / L were respectively higher than the maximum recommended field dose of 16.00 mg / L for dimethoate. Although cotton aphids collected from the field were resistant to dimethoate (resistance multiple was 12.17), the combined application of low-dose dimethoate (3.49 mg / L) and East Asian flower stink bug could significantly reduce the population of cotton aphids. On the 7th day after treatment, the aphid density decreased by 94.29%. Although the initial control effect of dimethoate on cotton aphids at the maximum recommended field dose reached 98.19%, the number of aphids rebounded over time. The combined application of low-dose dimethoate and East Asian flower stink bug can effectively control resistant cotton aphids, and a new integrated control strategy is proposed.

[0032] Those skilled in the art should understand that the above is only a specific embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention are included in the protection scope of the present invention.

Claims

1. A method for controlling resistant aphids by combining a predatory natural enemy, Ornithogalum spp., with diprofil, characterized in that: Spray dichlorvos evenly on crops, and then release the East Asian flower bug on the crops.

2. The method for controlling aphid pests by combining the predatory natural enemy Ornithogalum spp. and diprofiloxan as claimed in claim 1, characterized in that: The concentration of diprofenacil is between the aphid LC 20 Between the recommended doses.

3. The method for controlling aphid pests by combining the predatory natural enemy Ornithogalum spp. and diprofiloxan as claimed in claim 1, characterized in that: The control effect can be achieved by releasing the East Asian flower stink bug at a benefit-harm ratio of 1:300 or above.

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