Deflerization treatment method for leaf made of specimen
By using the method of warm -up and alkaline corrosion filter plates on the blades to rinse the combination of warm and alkaline corrosion filter plates, the problem of incomplete meat and low success rate of existing leaves and meat treatment of existing leaves, and realized efficient exfoliation, strong toughness and longness The effect of life.
Patent Information
- Application Number
- CN202411434780.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-15
- Publication Date
- 2025-01-24
AI Technical Summary
The existing leaf meat removal treatment methods have problems such as incomplete meat removal, low success rate, toughness and short lifespan of finished products.
Use pure alkaline water solution to warm the leaves, then rinse the leaf meat with gentle flowing water on the alkaline -resistant filter plate, combined with gardenia, spray, toothbrush, or towels, etc., and gradually remove the leaf meat, and perform neutralization, bleaching and air -drying treatment. In the end Use walnut oil care to mount.
The complete de -meat of the leaves is achieved, the meat rate and success rate are high, the finished product is strong, and the service life is long. It is suitable for specimen production.
Smart Images
Figure CN119344303A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of leaf specimen preparation, and in particular to a flesh removal processing method for leaf specimen preparation. Background Art
[0002] When making leaf specimens, the mesophyll of the leaves needs to be removed. Currently, there are three main methods for removing the mesophyll from leaves:
[0003] 1. Chemical corrosion method: Use chemical reagents such as sodium hydroxide solution to soak the leaves. Put the leaves in a solution of appropriate concentration and after a period of time at a certain temperature, the soft tissue of the leaves will be corroded, leaving only the harder parts such as the veins, which is easy to corrode excessively.
[0004] 2. Microbial decomposition method: Use microorganisms to decompose the mesophyll in the leaves. Place the leaves in a specific microbial environment, and the microorganisms will gradually decompose the mesophyll, but this method takes a long time and the microbial environment is difficult to control.
[0005] 3. Boiling method: Boil the leaves in water. High-temperature boiling can soften the leaf flesh, and then remove the leaf flesh by gently brushing, etc., but this method may affect the color and toughness of the leaves to a certain extent.
[0006] How to provide a fleshing method for leaf specimen production that can achieve complete fleshing, high success rate, strong toughness and long life of the finished product has become a research direction. Summary of the invention
[0007] The object of the present invention is to provide a method for removing flesh from leaves for specimen preparation, so as to solve the problems raised in the above-mentioned background technology.
[0008] In order to solve the above technical problems, the present invention provides a technical solution: a method for removing flesh from leaves for specimen preparation, which specifically comprises the following steps:
[0009] 1) Select leaves from the Araceae family, Araceae, and Araceae;
[0010] 2) Soda ash aqueous solution warm boiling treatment: put the selected leaves into a 5%-20% soda ash aqueous solution and boil at 70-90°C for 2-10 hours;
[0011] 3) Removing leaf flesh: After the leaves have been boiled, they are taken out and placed on an alkali corrosion-resistant filter plate with an area larger than the leaves. The soda ash aqueous solution is washed away with gentle running water to remove the leaf flesh and obtain the leaf veins;
[0012] 5) De-alkali: Soak the entire leaf vein in an inorganic acid solution for acid-base neutralization and de-alkali for 1.5-2.5 hours;
[0013] 6) Bleaching: Soak the leaf veins after acid-base neutralization treatment in hydrogen peroxide bleaching solution for bleaching, and remove them after more than 5 hours;
[0014] 6) Air drying: After the bleaching, remove the leaf veins from the board when the leaf veins are half dry to prevent the leaf veins from sticking to the board and damaging the leaf veins; after separation, place the leaf veins in a ventilated place to air dry until they are completely air dried;
[0015] 7) Care and mounting: Use walnut oil, spray it on the leaf veins in a spray form, and finally mount it.
[0016] Preferably, the soft item in step 3) is a toothbrush or a towel.
[0017] Preferably, when removing the leaf pulp in step 3), goggles and protective gloves are worn during the operation, and direct contact with the skin is not allowed.
[0018] Preferably, in step 3), the leaf pulp is removed: the leaves after the warm boiling treatment are fished out, placed on an alkali corrosion-resistant filter plate with an area larger than the leaves, and the soda ash aqueous solution is washed away with gently running water. The leaf pulp is first clamped with tweezers and torn off in pieces. The remaining leaf pulp that is difficult to tear off is locally rinsed with a spray, or lightly pressed with a soft object, pressed and hit multiple times to make the leaf pulp fall off, and then rinsed with running water to obtain leaf veins.
[0019] Preferably, the alkali corrosion resistant filter plate in step 3) is an ultra-high molecular weight polyethylene filter plate or a steel plate with hydrophobic holes.
[0020] Preferably, the inorganic acid in step 4) is acetic acid solution or citric acid solution.
[0021] Preferably, the sheet material used in step 6) is a polyethylene sheet material.
[0022] Preferably, the complete air drying in step 6) takes more than two days.
[0023] Preferably, the dealkali operation time in step 5) is 2 hours.
[0024] Preferably, in step 2), the selected leaves are placed in a 15% aqueous solution of pure alkali and boiled at 70-90° C. for 3 hours.
[0025] The advantages of the present invention are: easy to operate, complete fleshing can be achieved through gentle running water with tweezers, spray, toothbrush, towel, etc., the fleshing rate and success rate are high, walnut oil is used for care, the finished product has strong toughness and long service life, and it has good promotion prospects and economic value in the field of leaf specimen preparation technology. BRIEF DESCRIPTION OF THE DRAWINGS
[0026] Figure 1 It is a process flow chart of the present invention. DETAILED DESCRIPTION
[0027] For the purpose of the following detailed description, it should be understood that the present invention may adopt various alternative changes and step sequences, unless expressly specified to the contrary. In addition, except in any operating examples, or otherwise indicated, all numbers representing the amount of ingredients used in the specification and claims should be understood to be modified by the term "about" in all cases. Therefore, unless otherwise indicated, the numerical parameters set forth in the following specification and the attached claims are approximate values that vary according to the desired performance to be obtained by the present invention. At least it is not intended to limit the application of the doctrine of equivalents to the scope of the claims, and each numerical parameter should at least be interpreted according to the number of reported significant figures and by applying ordinary rounding techniques.
[0028] Notwithstanding that the numerical ranges and parameters setting forth the broad scope of the invention are approximations, the numerical values set forth in the specific examples are reported as precisely as possible. Any numerical value, however, inherently contains certain errors necessarily resulting from the standard deviation found in their respective testing measurements.
[0029] In addition, it should be understood that any numerical range described herein is intended to include all subranges subsumed therein. For example, a range of "1 to 10" is intended to include all subranges between (and including) the stated minimum value of 1 and the stated maximum value of 10, i.e., having a minimum value equal to or greater than 1 and a maximum value equal to or less than 10.
[0030] Example 1
[0031] A method for removing flesh from leaves for specimen preparation comprises the following steps:
[0032] 1) Select leaves from the Araceae family, Araceae, and Araceae;
[0033] 2) Soda ash aqueous solution warm boiling treatment: Put the selected leaves into 5% soda ash aqueous solution and boil at 90°C for 2 hours;
[0034] 3) Removing leaf flesh: After the leaves are boiled, they are taken out and placed on an alkali corrosion-resistant filter plate with an area larger than the leaves. The soda ash aqueous solution is washed away with gentle running water. The leaf flesh is first clamped with tweezers and torn off in pieces. The remaining leaf flesh that is difficult to tear off is locally washed with a spray, or lightly pressed with soft objects, pressed and hit several times to make the leaf flesh fall off, and then washed with running water to obtain the leaf veins. The removal rate and success rate are high;
[0035] 5) De-alkali: Soak the entire leaf vein in an inorganic acid solution for acid-base neutralization and de-alkali for 1.5 hours;
[0036] 6) Bleaching: Soak the leaf veins after acid-base neutralization treatment in hydrogen peroxide bleaching solution for bleaching, and remove them after more than 5 hours;
[0037] 6) Air drying: After the bleaching, remove the leaf veins from the board when the leaf veins are half dry to prevent the leaf veins from sticking to the board and damaging the leaf veins; after separation, place the leaf veins in a ventilated place to air dry until they are completely air dried;
[0038] 7) Care and mounting: Use walnut oil, spray it on the leaf veins in a spray form to effectively improve the toughness and life of the finished product, and finally mount it.
[0039] As a preferred implementation of this embodiment, the soft item in step 3) is a toothbrush.
[0040] As a preferred implementation of this embodiment, when removing the leaf pulp in step 3), wear goggles and protective gloves to perform the operation.
[0041] As a preferred implementation of this embodiment, the alkali corrosion-resistant filter plate in step 3) is an ultra-high molecular weight polyethylene filter plate.
[0042] As a preferred implementation of this embodiment, the inorganic acid in step 4) is acetic acid solution.
[0043] As a preferred implementation of this embodiment, the sheet material used in step 6) is a polyethylene sheet material.
[0044] As a preferred implementation of this embodiment, the complete air drying in step 6) takes more than two days.
[0045] Example 2
[0046] A method for removing flesh from leaves for specimen preparation comprises the following steps:
[0047] 1) Select leaves from the Araceae family, Araceae, and Araceae;
[0048] 2) Soda ash aqueous solution warm boiling treatment: Put the selected leaves into a 20% soda ash aqueous solution and boil at 70°C for 5 hours;
[0049] 3) Removing leaf flesh: After the leaves are boiled, they are taken out and placed on an alkali corrosion-resistant filter plate with an area larger than the leaves. The soda ash aqueous solution is washed away with gentle running water. The leaf flesh is first clamped with tweezers and torn off in pieces. The remaining leaf flesh that is difficult to tear off is locally washed with a spray, or lightly pressed with soft objects, pressed and hit several times to make the leaf flesh fall off, and then washed with running water to obtain the leaf veins. The removal rate and success rate are high;
[0050] 5) De-alkali: Soak the leaf veins in an inorganic acid solution for acid-base neutralization and de-alkali for 2 hours;
[0051] 6) Bleaching: Soak the leaf veins after acid-base neutralization treatment in hydrogen peroxide bleaching solution for bleaching, and remove them after more than 5 hours;
[0052] 6) Air drying: After the bleaching, remove the leaf veins from the board when the leaf veins are half dry to prevent the leaf veins from sticking to the board and damaging the leaf veins; after separation, place the leaf veins in a ventilated place to air dry until they are completely air dried;
[0053] 7) Care and mounting: Use walnut oil, spray it on the leaf veins in a spray form to effectively improve the toughness and life of the finished product, and finally mount it.
[0054] As a preferred implementation of this embodiment, the soft item in step 3) is a toothbrush.
[0055] As a preferred implementation of this embodiment, when removing the leaf pulp in step 3), wear goggles and protective gloves to perform the operation.
[0056] As a preferred implementation of this embodiment, the alkali corrosion-resistant filter plate in step 3) is a steel plate with hydrophobic holes.
[0057] As a preferred implementation of this embodiment, the inorganic acid in step 4) is citric acid solution.
[0058] As a preferred implementation of this embodiment, the sheet material used in step 6) is a polyethylene sheet material.
[0059] As a preferred implementation of this embodiment, the complete air drying in step 6) takes more than two days.
[0060] Example 3
[0061] A method for removing flesh from leaves for specimen preparation comprises the following steps:
[0062] 1) Select leaves from the Araceae family, Araceae, and Araceae;
[0063] 2) Soda ash aqueous solution warm boiling treatment: Put the selected leaves into 15% soda ash aqueous solution and boil at 70°C for 6 hours;
[0064] 3) Removing leaf flesh: After the leaves are boiled, they are taken out and placed on an alkali corrosion-resistant filter plate with an area larger than the leaves. The soda ash aqueous solution is washed away with gentle running water. The leaf flesh is first clamped with tweezers and torn off in pieces. The remaining leaf flesh that is difficult to tear off is locally washed with a spray, or lightly pressed with soft objects, pressed and hit several times to make the leaf flesh fall off, and then washed with running water to obtain the leaf veins. The removal rate and success rate are high;
[0065] 5) De-alkali: Soak the entire leaf vein in an inorganic acid solution for acid-base neutralization and de-alkali for 2.5 hours;
[0066] 6) Bleaching: Soak the leaf veins after acid-base neutralization treatment in hydrogen peroxide bleaching solution for bleaching, and remove them after more than 5 hours;
[0067] 6) Air drying: After the bleaching, remove the leaf veins from the board when the leaf veins are half dry to prevent the leaf veins from sticking to the board and damaging the leaf veins; after separation, place the leaf veins in a ventilated place to air dry until they are completely air dried;
[0068] 7) Care and mounting: Use walnut oil, spray it on the leaf veins in a spray form to effectively improve the toughness and life of the finished product, and finally mount it.
[0069] As a preferred implementation of this embodiment, the soft item in step 3) is a toothbrush.
[0070] As a preferred implementation of this embodiment, when removing the leaf pulp in step 3), wear goggles and protective gloves to perform the operation.
[0071] As a preferred implementation of this embodiment, the alkali corrosion-resistant filter plate in step 3) is an ultra-high molecular weight polyethylene filter plate.
[0072] As a preferred implementation of this embodiment, the inorganic acid in step 4) is acetic acid solution.
[0073] As a preferred implementation of this embodiment, the sheet material used in step 6) is a polyethylene sheet material.
[0074] As a preferred implementation of this embodiment, the complete air drying in step 6) takes more than two days.
[0075] Example 4
[0076] A method for removing flesh from leaves for specimen preparation comprises the following steps:
[0077] 1) Select leaves from the Araceae family, Araceae, and Araceae;
[0078] 2) Soda ash aqueous solution warm boiling treatment: Put the selected leaves into a 15% soda ash aqueous solution and boil at 80°C for 7 hours;
[0079] 3) Removing leaf flesh: After the leaves are boiled, they are taken out and placed on an alkali corrosion-resistant filter plate with an area larger than the leaves. The soda ash aqueous solution is washed away with gentle running water. The leaf flesh is first clamped with tweezers and torn off in pieces. The remaining leaf flesh that is difficult to tear off is locally washed with a spray, or lightly pressed with soft objects, pressed and hit several times to make the leaf flesh fall off, and then washed with running water to obtain the leaf veins. The removal rate and success rate are high;
[0080] 5) De-alkali: Soak the entire leaf vein in an inorganic acid solution for acid-base neutralization and de-alkali for 1.5 hours;
[0081] 6) Bleaching: Soak the leaf veins after acid-base neutralization treatment in hydrogen peroxide bleaching solution for bleaching, and remove them after more than 5 hours;
[0082] 6) Air drying: After the bleaching, remove the leaf veins from the board when the leaf veins are half dry to prevent the leaf veins from sticking to the board and damaging the leaf veins; after separation, place the leaf veins in a ventilated place to air dry until they are completely air dried;
[0083] 7) Care and mounting: Use walnut oil, spray it on the leaf veins in a spray form to effectively improve the toughness and life of the finished product, and finally mount it.
[0084] As a preferred implementation of this embodiment, the soft item in step 3) is a towel.
[0085] As a preferred implementation of this embodiment, when removing the leaf pulp in step 3), wear goggles and protective gloves to perform the operation.
[0086] As a preferred implementation of this embodiment, the alkali corrosion-resistant filter plate in step 3) is a steel plate with hydrophobic holes.
[0087] As a preferred implementation of this embodiment, the inorganic acid in step 4) is citric acid solution.
[0088] As a preferred implementation of this embodiment, the sheet material used in step 6) is a polyethylene sheet material.
[0089] As a preferred implementation of this embodiment, the complete air drying in step 6) takes more than two days.
[0090] Example 5
[0091] A method for removing flesh from leaves for specimen preparation comprises the following steps:
[0092] 1) Select leaves from the Araceae family, Araceae, and Araceae;
[0093] 2) Soda ash aqueous solution warm boiling treatment: Put the selected leaves into a 15% soda ash aqueous solution and boil at 90°C for 10 hours;
[0094] 3) Removing leaf flesh: After the leaves are boiled, they are taken out and placed on an alkali corrosion-resistant filter plate with an area larger than the leaves. The soda ash aqueous solution is washed away with gentle running water. The leaf flesh is first clamped with tweezers and torn off in pieces. The remaining leaf flesh that is difficult to tear off is locally washed with a spray, or lightly pressed with soft objects, pressed and hit several times to make the leaf flesh fall off, and then washed with running water to obtain the leaf veins. The removal rate and success rate are high;
[0095] 5) De-alkali: Soak the leaf veins in an inorganic acid solution for acid-base neutralization and de-alkali for 2 hours;
[0096] 6) Bleaching: Soak the leaf veins after acid-base neutralization treatment in hydrogen peroxide bleaching solution for bleaching, and remove them after more than 5 hours;
[0097] 6) Air drying: After the bleaching, remove the leaf veins from the board when the leaf veins are half dry to prevent the leaf veins from sticking to the board and damaging the leaf veins; after separation, place the leaf veins in a ventilated place to air dry until they are completely air dried;
[0098] 7) Care and mounting: Use walnut oil, spray it on the leaf veins in a spray form to effectively improve the toughness and life of the finished product, and finally mount it.
[0099] As a preferred implementation of this embodiment, the soft item in step 3) is a towel.
[0100] As a preferred implementation of this embodiment, when removing the leaf pulp in step 3), wear goggles and protective gloves to perform the operation.
[0101] As a preferred implementation of this embodiment, the alkali corrosion-resistant filter plate in step 3) is an ultra-high molecular weight polyethylene filter plate.
[0102] As a preferred implementation of this embodiment, the inorganic acid in step 4) is acetic acid solution.
[0103] As a preferred implementation of this embodiment, the sheet material used in step 6) is a polyethylene sheet material.
[0104] As a preferred implementation of this embodiment, the complete air drying in step 6) takes more than two days.
[0105] When the present invention is implemented, more than 2,000 plants belonging to the Araceae family, the order Araceae, the order Monocotyledons are selected.
[0106] The most popular leaves are: Monstera (including various genetic variants, such as White Monstera, Golden Monstera, etc. It also includes various regional species, such as Brazilian Monstera, etc., and the same applies below.)
[0107] Fairy Cave Monstera, Giant Cave Monstera, Brazilian Monstera, Imperial Anthurium, Flamingo King Anthurium, Flamingo Queen Anthurium, Shinia Anthurium, Tie Anthurium, Gorgeous Anthurium, Colorful Calla, Salian Calla, Philodendron, Dragon Scale Calla, Sulawesi Calla, Black Swan Calla, Arrow Leaf Calla, Senbei Calla, Green Velvet Calla, Elephant Ear Calla, etc.
[0108] The relevant parameters of leaves treated by microbial decomposition, water boiling and the fleshing method of the present invention are shown in Table 1
[0109] Table 1
[0110] Treatment Microbial decomposition method boiling method Meat removal processing method of the present invention Meat removal rate 89％ 93％ 99％ Success rate 56％ 69％ 95％ Tensile strength / MPa 7.5 9.3 13.6 Service life / year 3-4 7-8 ＞20
[0111] It can be clearly seen from the above table that the leaves treated by the fleshing method of the present invention are completely fleshed, with a high success rate, and the finished product has high tensile strength, strong toughness and long service life.
[0112] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.
Claims
1. A method for removing flesh from leaves for specimen preparation, characterized in that: The specific steps include: 1) Select leaves from the Araceae family, Araceae, and Araceae; 2) Soda ash aqueous solution warm boiling treatment: put the selected leaves into a 5%-20% soda ash aqueous solution and boil at 70-90°C for 2-10 hours; 3) Removing leaf flesh: After the leaves have been boiled, they are taken out and placed on an alkali corrosion-resistant filter plate with an area larger than the leaves. The soda ash aqueous solution is washed away with gentle running water to remove the leaf flesh and obtain the leaf veins; 5) De-alkali: Soak the entire leaf vein in an inorganic acid solution for acid-base neutralization and de-alkali for 1.5-2.5 hours; 6) Bleaching: Soak the leaf veins after acid-base neutralization treatment in hydrogen peroxide bleaching solution for bleaching, and remove them after more than 5 hours; 6) Air drying: After the bleaching, remove the leaf veins from the board when the leaf veins are half dry to prevent the leaf veins from sticking to the board and damaging the leaf veins; after separation, place the leaf veins in a ventilated place to air dry until they are completely air dried; 7) Care and mounting: Use walnut oil, spray it on the leaf veins in a spray form, and finally mount it.
2. A method for removing flesh from leaves for specimen preparation according to claim 1, characterized in that: In step 3), the soft item is a toothbrush or a towel.
3. A method for removing flesh from leaves for specimen preparation according to claim 2, characterized in that: When removing the leaf pulp in step 3), wear goggles and protective gloves to perform the operation.
4. A method for removing flesh from leaves for specimen preparation according to claim 3, characterized in that: In the step 3), the leaf pulp is removed: the leaves after the warm boiling treatment are fished out, placed on an alkali corrosion-resistant filter plate with an area larger than the leaves, and the soda ash aqueous solution is washed away with gently running water. The leaf pulp is first clamped with tweezers and torn off in pieces. The remaining leaf pulp that is difficult to tear off is locally rinsed with a spray, or lightly pressed with a soft object, pressed and hit multiple times to make the leaf pulp fall off, and then rinsed with running water to obtain leaf veins.
5. A method for removing flesh from leaves for specimen preparation according to claim 4, characterized in that: In the step 3), the alkali corrosion resistant filter plate is an ultra-high molecular weight polyethylene filter plate or a steel plate with hydrophobic holes.
6. A method for removing flesh from leaves for specimen preparation according to claim 5, characterized in that: In step 4), the inorganic acid is acetic acid solution or citric acid solution.
7. A method for removing flesh from leaves for specimen preparation according to claim 6, characterized in that: The sheet material used in step 6) is a polyethylene sheet material.
8. A method for removing flesh from leaves for specimen preparation according to claim 7, characterized in that: The step 6) takes more than two days to completely air dry.
9. A method for removing flesh from leaves for specimen preparation according to claim 8, characterized in that: The dealkali operation time in step 5) is 2 hours.
10. A method for removing flesh from leaves for specimen preparation according to any one of claims 1 to 9, characterized in that: In the step 2), the selected leaves are placed in a 15% aqueous solution of pure alkali and boiled at a temperature of 70-90° C. for 3 hours.