Whitening composition and application thereof

Abstract

The invention belongs to the field of cosmetics, and discloses a whitening composition and application thereof. The composition comprises the following specific components in percentage by weight: 80-90% of honey extract, 5-10% of papain and 5-10% of lactobacillus fermentation lysate. The composition can mildly promote cells containing melanin on the skin cuticle to fall off, accelerate cell metabolism of the cuticle, achieve the effects of whitening and smoothing the skin and meet the requirements of people for whitening and not damaging the skin barrier. Meanwhile, the invention further provides application and cosmetics of the composition.

Description

Technical Field

[0001] The invention relates to the field of cosmetics, and in particular to a whitening composition and application thereof. Background Art

[0002] Under normal circumstances, human epidermal cells divide and evolve continuously, and old or damaged keratinocytes fall off naturally to maintain a dynamic balance. When the barrier function is affected by external factors such as ultraviolet radiation, trauma, and excessive cleaning, it causes tanning, uneven skin tone, and rough skin surface, thus affecting the appearance. The normal skin epidermis renewal time is about 28 days. If the old keratinocytes are not peeled off normally and accumulate on the skin surface, the epidermis will be thickened, and the keratinocytes containing melanin will not be peeled off in time, resulting in darker and duller skin color, and even the formation of spots or tanning.

[0003] There are two main methods for skin rejuvenation and whitening on the market through exfoliation. One is physical exfoliation, which usually uses physical scrub particles to rub the skin to remove aging keratinocytes and accelerate the metabolism and renewal of new keratinocytes. Physical exfoliation may be used improperly and easily lead to excessive cleansing of keratin, making the skin more sensitive and even causing mechanical damage to the skin. The other is chemical exfoliation, which softens the stratum corneum by applying fruit acids such as tartaric acid, malic acid, lactic acid, citric acid, glucuronic acid, and salicylic acid, reduces the connection between keratinocytes, and causes the aging cells containing melanin on the surface of the skin to fall off, thereby achieving a smooth and fair skin effect. However, this type of fruit acid usually has a low pH and is more irritating. Excessive acid brushing can damage the skin barrier, causing the skin to dry and redden, and is not suitable for sensitive skin. Therefore, it is an urgent problem to develop a composition that can promote the renewal and shedding of melanin-containing keratinocytes on the skin while being gentle and non-irritating.

[0004] Therefore, the technical problem solved in this case is: how to develop a new whitening composition that accelerates keratin renewal without harming the skin. Summary of the invention

[0005] In order to solve the above problems, the present invention provides a whitening composition.

[0006] One of the purposes of the present invention is to provide a whitening composition to solve the technical problem that most exfoliating products on the market are highly irritating.

[0007] Another object of the present invention is to provide an application of a whitening composition, which is used in skin care products to help promote the metabolism of skin stratum corneum cells, promote the shedding of aging cells, whiten and lighten skin color, and make the skin smooth and flat.

[0008] In order to achieve the above object, the present invention adopts the following technical solution: A whitening composition, comprising the following components: a honey extract solution, a papain solution, and a lactobacillus fermentation lysate solution; The honey extract solution contains 14wt% to 18wt% of honey extract; The papain solution contains 6wt% to 10wt% of papain; The lactobacillus fermentation lysate solution contains 1wt% to 5wt% of lactobacillus fermentation lysate.

[0009] Preferably, the honey extract solution consists of 14wt% to 18wt% of honey extract, 15wt% to 25wt% of 1,3-propylene glycol, and the balance water; The papain solution is composed of 6wt% to 10wt% of papain, 55wt% to 65wt% of glycerol, and the balance of water; The lactobacillus fermentation lysate solution consists of 1wt% to 5wt% of lactobacillus fermentation lysate, 25wt% to 35wt% of 1,3-propylene glycol, 2wt% to 6wt% of 1,2-pentanediol, 0.05wt% to 0.1wt% of octanoylhydroxamic acid, and the remainder of water.

[0010] In the present invention, through the synergistic effect of honey extract, papain, and lactobacillus fermentation lysate: honey extract degrades corneocyte desmosomes, and papain enzymolyzes keratin, and the two act together on aging corneocytes, so that the corneocyte connection ability is greatly reduced, thereby achieving the corneocyte metabolism effect. Lactobacillus fermentation lysate maintains the acidic environment of the skin, supports honey extract and papain to exert their biological activities, and lactobacillus fermentation lysate has an antioxidant effect, can remove DPPH and lipid peroxidation, and achieve a whitening skin effect. Therefore, the synergistic effect of honey extract, papain, and lactobacillus fermentation lysate can significantly promote the metabolism of skin stratum corneum cells, exfoliate aging cells, whiten and lighten skin color, make the skin smooth and smooth, and do not hurt the skin.

[0011] Preferably, the whitening composition comprises, by weight percentage: 80% to 90% honey extract solution, 5% to 10% papain solution, and 5% to 10% lactobacillus fermentation lysate solution.

[0012] More preferably, the whitening composition comprises, by weight percentage: 83% to 87% honey extract solution, 6% to 8% papain solution, and 6% to 8% lactobacillus fermentation lysate solution.

[0013] In addition, the invention also discloses a use of the whitening composition in preparing skin care products.

[0014] Preferably, the whitening composition is contained in the skin care product in an amount of 1 wt% to 2 wt%.

[0015] Finally, the present invention also discloses a skin care product containing the whitening composition as described above.

[0016] Preferably, the skin care products include lotions, creams, sprays, essences, essential oils, cleansers, lotions, facial masks, and gels.

[0017] In other studies of the present invention, it was found that even if samples obtained by using single components or a combination of two components of honey extract / papain / lactobacillus fermentation lysate have the effect of promoting the renewal of skin stratum corneum cells, their efficacy on keratin renewal is poor and they are more irritating to the skin.

[0018] The invention is composed of three active substances: honey extract, papain and lactobacillus fermentation lysate.

[0019] Honey extract is extracted by natural fermentation and contains high concentration of gluconic acid. Compared with glycolic acid, lactic acid, malic acid, citric acid and other highly irritating acids, honey extract has a milder skin whitening effect. The gluconic acid contained in honey extract can activate tissue proteases to degrade the desmosomes connecting keratinocytes, so as to efficiently renew keratin and reduce the distribution of melanin in the stratum corneum. Gluconic acid has a strong attraction to water due to its polyhydroxy structure, so its moisturizing property is greatly improved. Honey extract can be used in a wide pH range and still maintains the effect of renewing keratin in the neutral range.

[0020] Papain is a cysteinyl protease extracted from papaya, which has the characteristics of high enzyme activity, good thermal stability, natural hygiene and safety. Papain has a strong hydrolysis ability for animal and plant proteins, peptides, esters, amides, etc. It acts on the stratum corneum of the skin, dissolves keratin, reduces the connection between cornified desmosomes, and thus gently and effectively exfoliates the skin, improves dull skin, and makes the skin tender and white.

[0021] Lactobacillus fermentation lysate contains organic acids, amino acids, vitamins, polysaccharides, small molecule peptides and biological enzymes. The organic acids can balance the pH of the skin surface, making the pH of the skin slightly acidic; the amino acids, vitamins and biological enzymes can repair epidermal cells, remove pigmentation, improve skin roughness and make the skin bright and elastic; the microbial polysaccharides contained in the Lactobacillus fermentation lysate also have antioxidant activity, and both neutral polysaccharides and acidic polysaccharides can effectively inhibit lipid peroxidation and powerfully remove DPPH, OH and peroxides.

[0022] Compared with the prior art, the present invention has the following beneficial effects: 1. The present invention adopts honey extract, papain, and lactobacillus fermentation lysate to compound three active substances, which synergistically achieve the effect of keratin renewal and reducing melanin distribution. Lactobacillus fermentation lysate can provide a more stable pH environment, which is more conducive to maintaining the structure and function of papain, and honey extract can also play its role when it is stable at a stable pH, so there is no incompatibility between the three.

[0023] 2. The present invention mainly uses honey extract, a natural extract, supplemented with papain, lactobacillus fermentation lysate and other components to ensure its keratin renewal and whitening effects while also having high mildness. DETAILED DESCRIPTION

[0024] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] The commodity source information of each raw material in the embodiment is as follows: Papain solution: Guangzhou Sainalong Fine Chemical Co., Ltd., containing 60wt% glycerol, 32wt% water, 8wt% papain, enzyme activity (U / ml) ≥80000; Honey extract solution: Guangzhou Hengtao Trading Co., Ltd., containing 64wt% water, 20wt% 1,3-propylene glycol, and 20wt% honey extract; Lactobacillus fermentation lysate solution: Baishimei Biotechnology (Zhejiang) Co., Ltd., containing 3wt% of lactobacillus fermentation lysate, 30wt% of 1,3-propylene glycol, 4wt% of 1,2-pentanediol, 0.075wt% of octanoylhydroxamic acid, and 65.925wt% of water; Lactobionic acid: Guangzhou Tinglan Biotechnology Co., Ltd., specific rotation (20°C, D) is +23°--+29°, molecular weight is 358.296 g / mol; Bromelain: Nanning Pangbo Bioengineering Co., Ltd., the concentration range is 500,000~1.2 million u / g, and the bromelain concentration used in the comparative example is 800,000 u / g; Streptococcus thermophilus fermentation product solution: Guangzhou Youran Biotechnology Co., Ltd., containing 10wt% of Streptococcus thermophilus fermentation product, 2wt% of 1,2-ethylene glycol, 1wt% of 1,2-pentanediol, and 87wt% of water; The production process of papain is summarized as follows: water immersion, filtration, glycerol extraction, concentration, preparation, inspection, packaging, and qualified products are put into the qualified product warehouse; The production process of the honey extract solution is summarized as follows: honey refining, fermentation, heating (inactivation and sterilization), cooling, adding preservative solvent 1,3-propylene glycol, and filtering and sterilization; The production process of the lactobacillus fermentation lysate solution is summarized as follows: seed activation, culture medium sterilization, inoculation, fermentation, fermentation end, centrifugation, bacterial cell collection, high-pressure crushing, centrifugation, membrane, preservative addition, inspection, and finished product; The production process of lactobionic acid is summarized as follows: dissolving lactose and distilled water, adding Au / C (activated carbon) catalyst, stirring, introducing oxygen, adjusting constant pH with sodium hydroxide, filtering with cation exchange resin, concentrating, cooling and crystallizing, inspecting, and storing; The production process of the thermophilic streptococcus fermentation product solution is summarized as follows: fermentation, crushing, purification, and packaging; It should be noted that the technical solution of the present invention can be implemented through other commercially available products and is not limited to the extracts provided by the above-mentioned manufacturers.

[0026] In the following examples and comparative examples, unless otherwise specified, the parts are parts by weight and the % are percentages by weight.

[0027] Example 1 A whitening composition comprises the following components in parts by weight: 90 parts of honey extract solution, 5 parts of papain solution, and 5 parts of lactobacillus fermentation lysate solution.

[0028] Example 2 A whitening composition comprises the following components in parts by weight: 85 parts of honey extract solution, 10 parts of papain solution, and 5 parts of lactobacillus fermentation lysate solution.

[0029] Example 3 A whitening composition comprises the following components in parts by weight: 85 parts of honey extract solution, 7.5 parts of papain solution, and 7.5 parts of lactobacillus fermentation lysate solution.

[0030] Example 4 A whitening composition comprises the following components in parts by weight: 85 parts of honey extract solution, 5 parts of papain solution, and 10 parts of lactobacillus fermentation lysate solution.

[0031] Example 5 A whitening composition comprises the following components in parts by weight: 80 parts of honey extract solution, 10 parts of papain solution, and 10 parts of lactobacillus fermentation lysate solution.

[0032] Comparative Example 1 A whitening composition comprises the following components in parts by weight: 50 parts of papain solution and 50 parts of lactobacillus fermentation lysate solution.

[0033] Comparative Example 2 A whitening composition comprises the following components in parts by weight: 90 parts of honey extract solution and 10 parts of lactobacillus fermentation lysate solution.

[0034] Comparative Example 3 A whitening composition comprises the following components in parts by weight: 90 parts of honey extract solution and 10 parts of papain solution.

[0035] Comparative Example 4 A composition comprises 85 parts of lactobionic acid, 7.5 parts of papain solution and 7.5 parts of lactobacillus fermentation lysate solution.

[0036] Comparative Example 5 A composition comprising 85 parts of honey extract solution, 7.5 parts of bromelain, and 7.5 parts of lactobacillus fermentation lysate solution.

[0037] Comparative Example 6 A composition comprising 85 parts of honey extract solution, 7.5 parts of papain solution and 7.5 parts of thermophilic streptococcus fermentation product solution.

[0038] Performance Testing 1. Exfoliation efficacy test Stratum corneum staining model test Detection principle: DHA is used for dyeing. The functional groups in DHA can react with the amino acids and amino groups of skin keratin to form brown polymers, which can produce an artificial brown color (Maillard reaction) on the skin. This reaction is only based on the stratum corneum. It will not affect the skin structure deeper than the stratum corneum. Therefore, it is used to simulate the dyeing of the stratum corneum on the surface of the skin. The exfoliating effect of the product is evaluated by the degree of change in the color difference value after the stratum corneum is dyed.

[0039] Specific test method: 33 healthy subjects aged 18-60 were screened, and the test areas on the arms of the subjects had no scars, pigmentation, erythema or other factors that could affect the results. The Skin-Colorimeter CL400 was used to test the initial ITA° values ​​of the four test areas. A single test area was measured 3 times in parallel and the average was taken (those with an initial skin color ITA°>20° in the test area were not included in the test). The test area was dyed with dihydroxyacetone (DHA). 48 hours after dyeing, the Skin-Colorimeter CL400 skin color tester was used to measure the ITA° value on the 0th day. A single test area was measured 3 times in parallel and the average was taken. Starting from the 0th day, the corresponding example samples were applied to each dyed test area, and each test substance was measured at (2.00±0.05) mg / cm 2 Apply evenly to the test area, twice a day, with an interval of no less than 4 hours between each application. The test period is 14 days. Collect the ITA° values ​​of each test on the 0th, 3rd, 7th, and 14th days of the formal test. Measure a single test area 3 times in parallel and take the average. The larger the ITA° value, the lighter the skin color, the more the stratum corneum metabolism, and the better the whitening and brightening effect. On the contrary, the darker the skin color, the slower the stratum corneum renewal rate.

[0040] Calculation of corneum renewal rate Data analysis The statistical analysis software was SPSS, and the independent sample t-test was used for comparison between the test samples and the negative control. The above statistical analyses were all two-tailed tests, and the significance level was α=0.05; P>0.05 indicated that there was no significant difference between the two groups; P<0.05 indicated that there was a significant difference between the two groups.

[0041] The compositions prepared in Examples 1-5 and Comparative Examples 1-6 were tested according to the above experiment, and the results are shown in Table 1; Table 1 ITA° value determination results of the compositions prepared in Examples 1-5 and Comparative Examples 1-6

[0042] Note: * indicates significant difference compared with the blank control group, P < 0.05.

[0043] According to the results in Table 1, we can see that: Compared with the blank control group, there was no significant difference between the groups. Compared with the blank control group, the ITA° of Examples 1-5 used for 14 days had significant differences (P < 0.05), which shows that the composition of the honey extract, papain, and lactobacillus fermentation lysate used in the present invention has obvious exfoliating effect and can significantly whiten and brighten the skin color. By comparing the ITA° value data on the 14th day of Example 1 with that of Comparative Example 1, it can be shown that the honey extract significantly whitens and brightens the skin color and promotes keratinocyte metabolism. Its mechanism is to degrade the keratinocyte connecting desmosomes, causing the aging cell structure to be not tight enough and easy to peel off. According to the 14-day ITA ° value data of comparative example 2-3, honey extract is retained, and it is matched with one of papain and lactobacillus fermentation lysate, and the effect of promoting keratinocyte metabolism is significantly reduced, this is due to: papain has an efficient hydrolysis effect on the protein of stratum corneum, plays a role in softening the keratinocyte skeleton-cytokeratin protein, so that it is dissolved; the components such as biological enzymes, organic acids, polysaccharides contained in lactobacillus fermentation lysate accelerate pigment cell metabolism and reduce pigment accumulation, and provide a beneficial pH environment for honey extract and papain, help maintain the activity of the first two, and enhance the keratin renewal effect of the first two. It is shown above that the composition of the present invention is the synergistic enhancement obtained by the composite of honey extract, papain and lactobacillus fermentation lysate 3 components to promote keratinocyte metabolism, and the three mutually synergize and enhance, and none of them is indispensable.

[0044] According to the data comparison between Example 3 and Comparative Examples 4-6, Comparative Example 4 uses lactobionic acid to replace the honey extract solution, Comparative Example 5 uses bromelain to replace the papain solution, and Comparative Example 6 uses the thermophilic Streptococcus fermentation product solution to replace the lactobacillus fermentation lysate solution, all of which have a certain effect of promoting stratum corneum metabolism, but the effect is much weaker than that of Example 3. It can be reasonably inferred that the present invention uses the honey extract, papain, and lactobacillus fermentation lysate to produce a synergistic effect of improving keratinocyte metabolism.

[0045] Stratum corneum renewal rate Corneocyte renewal rate: refers to the degree of completion of the process from the stratum corneum being dyed to the complete fading of the skin, that is, the degree of completion of the process in which the entire epidermal stratum corneum is shed and replaced by undyed, newly divided and mature epidermal cells. It is calculated according to formula (1).

[0046] Corneocyte renewal rate = (A i -A0) / (A -1 -A0)×100%, formula (1); in, A -1 is the initial ITA° value of the skin before treatment with DHA, A0 is the ITA° baseline value of the stained area after DHA treatment, Ai It is the ITA° value of the dyed area at each test time point after DHA treatment. The higher the keratinocyte renewal rate, the better the effect of promoting keratinocyte metabolism. The relevant test results are shown in Table 2.

[0047] Table 2 Test results of keratinocyte renewal rate Group 14-day use corneocyte renewal rate / % Blank control group 50.43 Example 1 <![CDATA[75.38 * ]]> Example 2 <![CDATA[75.69 * ]]> Example 3 <![CDATA[75.71 * ]]> Example 4 <![CDATA[75.22 * ]]> Example 5 <![CDATA[75.42 * ]]> Comparative Example 1 49.85 Comparative Example 2 56.28 Comparative Example 3 56.63 Comparative Example 4 53.21 Comparative Example 5 53.02 Comparative Example 6 53.10

[0048] According to the results in Table 2: The data of Examples 1-5 and Comparative Examples 1-6 are compared. Examples 1-5 and Comparative Examples 1-6 both have a promoting effect on the renewal rate of keratinocytes, but the extent of their promotion is different; the keratinocyte renewal rate of Examples 1-5 is significantly higher than that of the blank group, with a significant difference (P < 0.05), indicating that the composition scheme of the honey extract, papain, and lactobacillus fermentation lysate used in the present invention can accelerate skin metabolism and accelerate the renewal rate of keratinocytes. By comparing the data of Example 1 and Comparative Example 1, the keratinocyte renewal rate of Example 1 is 75.38%, which is higher than the keratin renewal rate of Comparative Example 1 of 49.85%. It can be inferred that honey extract plays an important role in promoting keratinocyte renewal and promoting skin metabolism. By comparing the data of Example 1 with Comparative Examples 2-3, retaining the honey extract component, lacking any one of the components of papain and lactobacillus fermentation lysate, the effect of promoting keratinocyte renewal will be weakened, indicating that the composition of the present invention is a compound of three components of honey extract, papain, and lactobacillus fermentation lysate to exert the maximum synergistic exfoliation effect.

[0049] According to the data comparison of Example 3 and Comparative Examples 4-6, Comparative Example 4 uses lactobionic acid to replace the honey extract solution, Comparative Example 5 uses bromelain to replace the papain solution, and Comparative Example 6 uses a thermophilic streptococcus fermentation product solution to replace the lactobacillus fermentation lysate solution. The keratin renewal rates of Comparative Examples 4-6 are 53.21%, 53.02%, and 53.10%, respectively, which are lower than 75.71% of Example 3. They all have a certain effect of promoting the renewal metabolism of the stratum corneum, but their effect is much weaker than that of Example 3. It can be inferred that the high content of gluconic acid in the honey extract has a smaller molecular weight than lactobionic acid and a stronger penetration ability; the biological activity of papain is better than that of bromelain; the lactobacillus fermentation lysate contains a variety of nutrients, among which microbial polysaccharides have antioxidant effects, and the rich organic acids provide a weakly acidic environment suitable for honey extract and papain to play a role. Most of the components in the thermophilic streptococcus fermentation product are organic acids, and their pH is about 5, which is acidic, so it is compounded with honey extract and papain, and does not achieve a better keratin renewal effect. The above description shows that the present invention uses honey extract, papain, and lactobacillus fermentation lysate to produce a synergistic effect of improving keratinocyte renewal.

[0050] 2. Whitening efficacy test Test method: Referring to the test method of cosmetics for removing spots and whitening effects (first method), 35 subjects aged 18-60 years old with no pigmentation, inflammation, scars or other factors affecting the test results on the back skin were selected. A daylight simulator was used to build a skin melanization model for the test site. On the day of modeling, the skin color of each test area was tested with instruments for ITA° and melanin content. The subjects began to use the samples once in the morning and evening in each melanization test area for 4 consecutive weeks. ITA° and melanin content data were collected in the 4th week.

[0051] Sample configuration: according to Table 3, different essences containing different whitening compositions of Example 4 and their application contents are configured; Table 3 Formulas of essences with different contents Component name Sample 1 (negative control) Sample 2 Sample 3 Sample 4 Sample 5 Sample 6 Sample 7 water To 100% To 100% To 100% To 100% To 100% To 100% To 100% Example 4 0 0.5% 1% 1.5% 2% 2.5% 3% Thickener 0.3% 0.3% 0.3% 0.3% 0.3% 0.3% 0.3% pH Adjuster Moderate Moderate Moderate Moderate Moderate Moderate Moderate preservative Moderate Moderate Moderate Moderate Moderate Moderate Moderate total 100% 100% 100% 100% 100% 100% 100%

[0052] Samples 1-7 were tested according to the above-mentioned whitening efficacy test method, and the results are shown in Tables 4 and 5; Table 4 ITA° comparison results of test samples Group Before use ITA° 4 weeks of use ITA° ITA°Change Rate / % P-value Sample 1 20.15 23.916035 18.69 ＞0.05 Sample 2 20.36 24.582664 20.74 ＞0.05 Sample 3 21.67 31.022772 43.16 ＜0.05 Sample 4 20.78 30.230744 45.48 ＜0.05 Sample 5 21.43 31.319945 46.15 ＜0.05 Sample 6 21.07 30.724274 45.82 ＜0.05 Sample 7 20.69 30.068777 45.33 ＜0.05

[0053] Table 5 Comparison results of MI values ​​of test samples Group MI value before use Use 4-week MI values MI value change rate P-value Sample 1 197.16 166.718496 -15.44 ＞0.05 Sample 2 197.85 163.93851 -17.14 ＞0.05 Sample 3 197.64 160.365096 -18.86 ＜0.05 Sample 4 196.58 159.367406 -18.93 ＜0.05 Sample 5 196.37 158.961515 -19.05 ＜0.05 Sample 6 196.61 159.332744 -18.96 ＜0.05 Sample 7 197.55 160.509375 -18.75 ＜0.05

[0054] According to Table 4 and Table 5, we can know that: Compared with sample 1 (negative control), samples 2-7 all showed the effect of brightening the skin and reducing the melanin content; sample 2 had a certain brightening effect but no significant difference (P>0.05), and samples 3-7 had significant differences in ITA° and MI (P<0.05). As the content of the composition of Example 4 increased, its whitening effect increased; among them, the enhancement of the effect of samples 6-7 tended to be gentle, and there was no significant improvement compared with sample 5. The above whitening efficacy test experiments show that the preferred amount of the whitening composition provided by the present invention in skin care products is 1-2wt%. The efficacy of the product below 1wt% is not obvious, and the efficacy of the product above 2wt% is not significantly improved.

[0055] 3. Mildness test Patch test Specific test method: 34 volunteers aged 18-60 who meet the test requirements are selected as test subjects. The test area is gently wiped with a dry soft tissue, 0.025g of the sample to be tested is added to the spot tester, and the spot tester with the corresponding number is attached to the corresponding test area according to the preset sample position. The spot tester is quickly attached to the corresponding position from top to bottom, gently pressed and smoothed, and applied to the skin for 24 hours. After that, the tester removes the spot tester and marks it with a skin marker. 0.5h after removing the patch (after the indentation disappears), the results of naked eye observation are recorded.

[0056] Judgment criteria: Grading criteria for skin reactions in skin occlusive patch tests: Grade 0: negative reaction, no adverse reaction; Grade 1: suspicious reaction, only slight erythema; Grade 2: weak positive reaction (erythema reaction); erythema, infiltration, edema, and may have papules; Grade 3: strong positive reaction (herpes reaction); erythema, infiltration, edema, papules, and herpes; the reaction may extend beyond the test area; Grade 4: extremely strong positive reaction (confluent herpes reaction); obvious erythema, severe infiltration, edema, and confluent herpes; the reaction extends beyond the test area.

[0057] The examples were subjected to skin patch tests according to the above test method, and the results are shown in Table 6; Table 6 Skin patch test results Sample name Level 0 reaction Level 1 reaction Second level reaction Grade 3 reaction Grade 4 reaction Example 1 0 0 0 0 0 Example 2 0 0 0 0 0 Example 3 0 0 0 0 0 Example 4 0 0 0 0 0 Example 5 0 0 0 0 0 Comparative Example 1 0 0 0 0 0 Comparative Example 2 0 0 0 0 0 Comparative Example 3 0 0 0 0 0 Comparative Example 4 1 1 0 0 0 Comparative Example 5 2 1 0 0 0 Comparative Example 6 3 0 0 0 0

[0058] It can be concluded from the patch test statistical results that neither Examples 1-5 nor Comparative Examples 1-3 showed positive patch reactions, that is, the combination of honey extract, papain and lactobacillus fermentation lysate has low skin irritation and high safety.

[0059] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be considered exemplary and non-restrictive in all respects, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention.

Claims

1. A whitening composition, characterized in that: The whitening composition comprises the following components by weight percentage: 80 wt % to 90 wt % of a honey extract solution, 5 wt % to 10 wt % of a papain solution, and 5 wt % to 10 wt % of a lactobacillus fermentation lysate solution; The honey extract solution contains 14wt% to 18wt% of honey extract; The papain solution contains 6wt% to 10wt% of papain; The lactobacillus fermentation lysate solution contains 1wt% to 5wt% of lactobacillus fermentation lysate.

2. The whitening composition according to claim 1, characterized in that The honey extract solution is composed of 14wt% to 18wt% of honey extract, 15wt% to 25wt% of 1,3-propylene glycol, and the balance of water; The papain solution is composed of 6wt% to 10wt% of papain, 55wt% to 65wt% of glycerol, and the balance of water; The lactobacillus fermentation lysate solution consists of 1wt% to 5wt% of lactobacillus fermentation lysate, 25wt% to 35wt% of 1,3-propylene glycol, 2wt% to 6wt% of 1,2-pentanediol, 0.05wt% to 0.1wt% of octanoylhydroxamic acid, and the remainder of water.

3. Use of the whitening composition according to claim 1 or 2 in preparing skin care products.

4. The use according to claim 3, characterized in that The skin care product is a skin care product for whitening.

5. A skin care product, characterized in that: Contains the whitening composition as claimed in claim 1 or 2.

6. The skin care product according to claim 5, characterized in that The skin care product contains 1wt% to 2wt% of the whitening composition.

7. The skin care product according to claim 6, characterized in that The skin care products include lotions, creams, sprays, essences, essential oils, cleansers, lotions, facial masks, and gels.

Images