Beta-carotene microcapsule powder and preparation method thereof

Abstract

The invention belongs to the technical field of microcapsules, and particularly relates to beta-carotene microcapsule powder and a preparation method thereof.The beta-carotene microcapsule powder is prepared from, by mass, 1-5 parts of beta-carotene, 5-15 parts of vegetable oil, 0.1-5 parts of antioxidant, 2-5 parts of emulsifier, 40-60 parts of wall material, 1-5 parts of stabilizer, 0.1-5 parts of antioxidant synergist and 20-30 parts of water-soluble modified starch; according to the method, isomerization of beta-carotene in the heating process is prevented, higher bioavailability is achieved, the embedding rate can reach 99% through secondary embedding, water solubility is excellent, and storage and processing of the product are better facilitated.

Description

Technical Field

[0001] The invention belongs to the technical field of microcapsules, and in particular relates to beta-carotene microcapsule powder and a preparation method thereof. Background Art

[0002] β-carotene is a food additive permitted by the state, which has many physiological functions such as supplementing vitamin A, improving vision, enhancing nutrition, improving immunity, preventing cancer, etc. In recent years, the research on the effect of β-carotene on the human body has been very active. A large number of studies have shown that many biological functions of β-carotene are closely related to human health. It is widely used in food, cosmetics, feed, health care products and other industries as a safe and healthy food additive, nutritional supplement, colorant and antioxidant.

[0003] β-carotene is a fat-soluble hydrocarbon that is insoluble in water, slightly soluble in vegetable oil, and easily soluble in chloroform. Its melting point is around 180°C. Natural β-carotene is mainly an all-trans structure, and also contains 9-cis, 13-cis, and 15-cis structures to varying degrees. Most research results show that the all-trans configuration is the active ingredient of β-carotene that is beneficial to human health. Due to the presence of multiple unsaturated double bonds in the β-carotene molecule, its chemical properties are extremely active and are easily affected by temperature, light, oxygen, moisture, and other factors to accelerate degradation and isomerization. Therefore, it brings great troubles to the storage, transportation, and application of β-carotene. Microencapsulation technology is one of the main ways to solve this problem. Using microencapsulation technology to embed β-carotene in macromolecular membrane materials can protect the core substance, thereby improving the stability of β-carotene and making it easier to store and transport.

[0004] Invention patent CN113841895A places β-carotene crystals in a grinder, then adds grinding beads, dispersants, stabilizers, antioxidants and purified water for grinding, then emulsifies and homogenizes, and finally spray-dries to prepare β-carotene microcapsule powder. This method directly adds water to the ball mill and then emulsifies and homogenizes, and β-carotene is insoluble in water and easily precipitates, which is not conducive to storage.

[0005] Invention patent CN104719894A discloses a β-carotene microcapsule and its preparation process, wherein β-carotene is placed in an oil bath at 120-180°C for 3-10 minutes to obtain an oil phase, which is then sheared and mixed with a water phase and then spray-dried to obtain β-carotene microcapsule powder. This method does not fully protect β-carotene and is prone to isomerization of β-carotene.

[0006] The above invention patents all have the defect that the β-carotene microcapsule powder has poor stability. Therefore, the present invention provides a β-carotene microcapsule powder with strong stability. Summary of the invention

[0007] The present invention aims to overcome at least one defect of the above-mentioned prior art and provide a β-carotene microcapsule powder and a preparation method thereof, so as to solve the problem of poor stability of the existing β-carotene microcapsule powder.

[0008] One of the purposes of the present invention is to provide a beta-carotene microcapsule powder, which comprises the following components in parts by weight: 1 to 5 parts of beta-carotene, 5 to 15 parts of vegetable oil, 1.5 to 3 parts of antioxidant, 2 to 3 parts of emulsifier, 40 to 60 parts of wall material, 1 to 2 parts of stabilizer, 0.5 to 2 parts of antioxidant synergist and 20 to 30 parts of water-soluble modified starch.

[0009] Furthermore, the antioxidant is any one of BHA, BHT, TBHQ, PG, natural VE and ascorbic acid, or a mixture of several of them.

[0010] Preferably, the antioxidant is a combination of BHA+BHT, wherein the mass ratio of BHA to BHT is 2-5:1; or, the antioxidant is a combination of BHA+PG, wherein the mass ratio of BHA to PG is 2-5:1.

[0011] Furthermore, the emulsifier is any one of Tween 60, Tween 80, propylene glycol fatty acid ester, sodium caseinate and enzymatically hydrolyzed soybean lecithin, or a mixture of several of them; preferably, the emulsifier is a combination of Tween 60+enzymatically hydrolyzed soybean lecithin; or, the emulsifier is a combination of Tween 80+enzymatically hydrolyzed soybean lecithin.

[0012] Furthermore, the vegetable oil is any one of soybean oil, corn oil, rapeseed oil, safflower seed oil and peanut oil, or a mixture of several of them; the wall material is any one of rice bran protein, lotus seed protein isolate, cyclodextrin, gum arabic and isomaltooligosaccharide, or a mixture of several of them.

[0013] Furthermore, the stabilizer is any one of sodium pyrophosphate, sodium hexametaphosphate and sodium tripolyphosphate; the antioxidant synergist is any one of disodium EDTA and citric acid or a mixture of the two; and the water-soluble modified starch is sodium octenyl succinate starch.

[0014] The second object of the present invention is to provide a method for preparing the β-carotene microcapsule powder, comprising the following steps:

[0015] S1, mixing β-carotene, vegetable oil, antioxidant and antioxidant synergist and grinding to obtain a stable β-carotene suspension;

[0016] S2, heat-dissolving the β-carotene suspension at 160-200°C for 10-40s and then cooling to 60-100°C;

[0017] S3, adding an emulsifier and performing secondary heat melting for 2 to 10 minutes at 100 to 140° C. to fully dissolve the β-carotene in the vegetable oil to obtain a β-carotene oil solution;

[0018] S4, mixing the β-carotene oil solution, the wall material and the stabilizer to obtain a β-carotene emulsion;

[0019] S5. Add water-soluble modified starch to granulate the β-carotene emulsion to obtain β-carotene microcapsule powder.

[0020] In this solution, β-carotene is instantly heat-melted under the synergistic antioxidant effect of BHA+BHT / PG+antioxidant synergist, and then heat-melted in vegetable oil for the second time through Tween 60 / Tween 80+enzymatic soybean lecithin, which not only prevents β-carotene from isomerization during heating, has higher biological effectiveness, but also completely dissolves β-carotene in vegetable oil, increasing the stability of the product. In addition, the wall material is used for embedding, and the water-soluble modified starch is used for secondary embedding in a cycle. The embedding rate of the β-carotene microcapsule powder prepared by the present invention can be as high as 99%, and it is fully water-soluble, which is more conducive to the storage and processing of the product.

[0021] Furthermore, in step S1, the particle size of the β-carotene powder after grinding by colloid mill is ≤1 micron.

[0022] Further, in step S4, the β-carotene oil solution, wall material and stabilizer are emulsified and homogenized, and the homogenization pressure is controlled at 60-100 MPa; and / or, in step S5, the β-carotene emulsion is sent to a spray drying granulation device for granulation, and the air inlet temperature of the spray drying granulation device is 100-125°C.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] 1. The present invention uses a combination of BHA+BHT / PG+antioxidant synergist to perform synergistic antioxidant effects on β-carotene, thereby preventing β-carotene from isomerizing during heating and having higher biological effectiveness. At the same time, Tween 60 / Tween 80+enzymatically hydrolyzed soybean lecithin is secondary heat-melted in vegetable oil to completely dissolve β-carotene in the vegetable oil, thereby increasing the stability of the product.

[0025] 2. The present invention does not add any organic solvent during the preparation of the β-carotene microcapsule powder, thereby improving the safety of the product.

[0026] 3. The present invention uses wall materials to embed β-carotene, and uses water-soluble modified starch for secondary embedding. The embedding rate can be as high as 99%, and the β-carotene microcapsule powder is fully water-soluble, and the prepared β-carotene microcapsule powder is more conducive to storage and processing. DETAILED DESCRIPTION

[0027] One of the purposes of the present invention is to provide a beta-carotene microcapsule powder, which comprises the following components in percentage by mass: 1 to 5 parts of beta-carotene, 5 to 15 parts of vegetable oil, 1.5 to 3 parts of antioxidant, 2 to 3 parts of emulsifier, 40 to 60 parts of wall material, 1 to 2 parts of stabilizer, 0.5 to 2 parts of antioxidant synergist and 20 to 30 parts of water-soluble modified starch.

[0028] In a specific implementation, the antioxidant is any one of BHA, BHT, TBHQ, PG, natural VE and ascorbic acid, or a mixture of several of them.

[0029] Preferably, the antioxidant is a combination of BHA+BHT, wherein the mass ratio of BHA to BHT is 2-5:1; or, the antioxidant is a combination of BHA+PG, wherein the mass ratio of BHA to PG is 2-5:1.

[0030] Compared with using only a single antioxidant, the combination of BHA+BHT or BHA+PG has a higher protective ability for β-carotene, preventing β-carotene from isomerizing during high-temperature dissolution and reducing the beneficial effects of β-carotene. When the ratio of BHA:BHT or BHA:PG is lower or higher than 2-5:1, its antioxidant capacity decreases to a certain extent.

[0031] In specific implementation, the emulsifier is any one or a mixture of Tween 60, Tween 80, propylene glycol fatty acid ester, sodium caseinate and enzymatically hydrolyzed soybean lecithin; preferably, the emulsifier is a combination of Tween 60 + enzymatically hydrolyzed soybean lecithin; or, the emulsifier is a combination of Tween 80 + enzymatically hydrolyzed soybean lecithin. Among them, the enzymatically hydrolyzed soybean lecithin is made from soybean concentrated lecithin and degraded by lipase. The lipase is preferably at least one of Novozym 435 and Lipozyme TLIM. The lipase comes from Novozymes of Denmark and has an HLB value of ≥9.

[0032] Preferably, the vegetable oil is any one of soybean oil, corn oil, rapeseed oil, safflower oil and peanut oil, or a mixture of several of them; the wall material is any one of rice bran protein, lotus seed protein isolate, cyclodextrin, gum arabic and isomaltooligosaccharide, or a mixture of several of them.

[0033] In a specific implementation, the stabilizer is any one of sodium pyrophosphate, sodium hexametaphosphate and sodium tripolyphosphate; the antioxidant synergist is any one of disodium EDTA and citric acid or a mixture of the two; and the water-soluble modified starch is sodium octenyl succinate starch.

[0034] The second object of the present invention is to provide a method for preparing the β-carotene microcapsule powder, comprising the following steps:

[0035] S1, mixing β-carotene, vegetable oil, antioxidant and antioxidant synergist and grinding to obtain a stable β-carotene suspension;

[0036] S2, heat-dissolving the β-carotene suspension at 160-200°C for 10-40s and then cooling to 60-100°C;

[0037] S3, adding an emulsifier and performing secondary heat melting for 2 to 10 minutes at 100 to 140° C. to fully dissolve the β-carotene in the vegetable oil to obtain a β-carotene oil solution;

[0038] S4, mixing the β-carotene oil solution, the wall material and the stabilizer to obtain a β-carotene emulsion;

[0039] S5. Add water-soluble modified starch to granulate the β-carotene emulsion to obtain β-carotene microcapsule powder.

[0040] In this solution, β-carotene is instantly heat-melted under the synergistic antioxidant effect of BHA+BHT / PG+antioxidant synergist, and then heat-melted in vegetable oil for the second time through Tween 60 / Tween 80+enzymatic soybean lecithin, which not only prevents β-carotene from isomerization during heating, has higher biological effectiveness, but also completely dissolves β-carotene in vegetable oil, increasing the stability of the product. In addition, the wall material is used for embedding, and the water-soluble modified starch is used for secondary embedding in a cycle. The embedding rate of the β-carotene microcapsule powder prepared by the present invention can be as high as 99%, and it is fully water-soluble, which is more conducive to the storage and processing of the product.

[0041] In specific implementation, in step S1, the particle size of the β-carotene powder after grinding by colloid mill is ≤1 micron.

[0042] In specific implementation, in step S4, the β-carotene oil solution, wall material and stabilizer are emulsified and homogenized, and the homogenization pressure is controlled at 60-100 MPa; and / or, in step S5, the β-carotene emulsion is sent to a spray drying granulation device for granulation, and the air inlet temperature of the spray drying granulation device is 100-125°C.

[0043] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0044] The experimental methods used in the following examples are conventional methods unless otherwise specified. The materials, reagents, methods and instruments used are conventional materials, reagents, methods and instruments in the art unless otherwise specified, and can be obtained through commercial channels by those skilled in the art.

[0045] Example 1

[0046] This embodiment provides a β-carotene microcapsule powder, and the specific preparation method is as follows:

[0047] S1. Put 1.5 parts of β-carotene, 7 parts of soybean oil, 1 part of BHA, 0.5 parts of BHT and 1 part of disodium EDTA into a colloid mill and grind them thoroughly to obtain a stable β-carotene suspension;

[0048] S2, β-carotene suspension was heated at 180℃ for 30s and then rapidly cooled to 80℃;

[0049] S3, adding 2 parts of Tween 60 and 1 part of enzymatically hydrolyzed soybean lecithin for secondary heat melting at 125°C for 6 minutes to fully dissolve the β-carotene in the vegetable oil to obtain a β-carotene oil solution;

[0050] S4, β-carotene oil solution, 42 parts of cyclodextrin, 12 parts of rice bran protein and 2 parts of sodium pyrophosphate were mixed and stirred, and emulsified and homogenized, and the homogenization pressure was controlled to 80Mpa to obtain a β-carotene emulsion;

[0051] S5. Send the β-carotene emulsion and 30 parts of sodium starch octenylsuccinate to a spray drying granulation device for granulation at an inlet air temperature of 120° C. to obtain β-carotene microcapsule powder.

[0052] Example 2

[0053] This embodiment provides a β-carotene microcapsule powder, and the specific preparation method is as follows:

[0054] S1. Put 1.5 parts of β-carotene, 7 parts of soybean oil, 1.2 parts of BHA, 0.3 parts of BHT and 1 part of disodium EDTA into a colloid mill and grind them thoroughly to obtain a stable β-carotene suspension;

[0055] S2, β-carotene suspension was heated at 180℃ for 30s and then rapidly cooled to 80℃;

[0056] S3, adding 2 parts of Tween 60 and 1 part of enzymatically hydrolyzed soybean lecithin for secondary heat melting at 125°C for 6 minutes to fully dissolve the β-carotene in the vegetable oil to obtain a β-carotene oil solution;

[0057] S4, β-carotene oil solution, 42 parts of cyclodextrin, 12 parts of rice bran protein and 2 parts of sodium pyrophosphate were mixed and stirred, and emulsified and homogenized, and the homogenization pressure was controlled to 80Mpa to obtain a β-carotene emulsion;

[0058] S5. Send the β-carotene emulsion and 30 parts of sodium starch octenylsuccinate to a spray drying granulation device for granulation at an inlet air temperature of 120° C. to obtain β-carotene microcapsule powder.

[0059] Example 3

[0060] This embodiment provides a β-carotene microcapsule powder, and the specific preparation method is as follows:

[0061] S1. Put 1.5 parts of β-carotene, 10.5 parts of rapeseed oil, 2 parts of BHA, 1 part of PG and 1 part of citric acid into a colloid mill and grind them thoroughly to obtain a stable β-carotene suspension;

[0062] S2, β-carotene suspension was heated at 175℃ for 25s and then rapidly cooled to 75℃;

[0063] S3, adding 1 part of Tween 80 and 1 part of enzymatically hydrolyzed soybean lecithin to perform secondary heat melting for 8 minutes at 120°C to fully dissolve the β-carotene in the vegetable oil to obtain a β-carotene oil solution;

[0064] S4, mixing the β-carotene oil solution, 40 parts of gum arabic, 15 parts of lotus seed protein isolate and 2 parts of sodium hexametaphosphate, and performing emulsification and homogenization treatment, and controlling the homogenization pressure to 75 MPa to obtain a β-carotene emulsion;

[0065] S5. Send the β-carotene emulsion and 25 parts of sodium starch octenylsuccinate to a spray drying granulation device for granulation at an inlet air temperature of 120° C. to obtain β-carotene microcapsule powder.

[0066] Comparative Example

[0067] A blank group and a control group were set up for experimental data reference, wherein the blank group was a sample prepared by adsorbing β-carotene on a silica carrier;

[0068] Control group 1 is a sample prepared according to the method in Example 1 of CN113841895A;

[0069] Control group 2 is a sample prepared according to the method in Example 1 of CN104719894A;

[0070] Control group 3 is a sample prepared according to the method in Example 1 of this patent, except that disodium EDTA is not added in step S1;

[0071] Control group 4 is a sample prepared according to the method in Example 1 of this patent, except that the content of BHA:BHT in step S1 is 8:1;

[0072] Control group 5 is a sample prepared according to the method in Example 1 of this patent, except that the content of BHA:BHT in step S1 is 1:2;

[0073] Control group 6 is a sample prepared according to the method in Example 1 of this patent, except that the amount of BHA added in step S1 is 1.5 parts, and no BHT is added;

[0074] Control group 7 is a sample prepared according to the method in Example 1 of this patent, with the only difference being that enzymatically hydrolyzed soybean lecithin is not added in step S3.

[0075] Test Example 1

[0076] Sensory performance test of β-carotene microcapsule powder

[0077] Test samples: blank group, control group 1-7 and β-carotene microcapsule powder prepared in Example 1-3;

[0078] Test standard: blank group, control group and embodiment, each group of samples were tested in equal amounts, and each group of samples was divided into three parallel samples, and the sensory performance analysis was performed on them respectively, and the average value was calculated by repeating three times. The average value of the three parallel samples is shown in Table 1.

[0079] Table 1 Sensory performance analysis of β-carotene microcapsule powder

[0080]

[0081]

[0082] It can be seen from the test results in Table 1 that the β-carotene microcapsule powder prepared by the technical solution of the present invention has a better embedding rate and better water solubility than the comparative example.

[0083] By comparing Examples 1-3 and Control Group 1-2, it can be seen that the β-carotene microcapsule powder prepared by the technical solution of the present invention is fully water-soluble, and the embedding rate is above 99%, while the β-carotene microcapsule powder prepared by Control Group 1-2 still has fine particles after being dissolved in water, and the embedding rate is below 90%. The β-carotene microcapsule powder prepared by Examples 1-3 has better embedding rate and water solubility than that of Control Group 1-2.

[0084] From the comparison between Example 1 and Control Group 7, it can be seen that, compared with Example 1, Control Group 7 does not add enzymatic soybean lecithin, Example 1 is fully soluble in water, and the embedding rate is above 99%. After the Control Group 7 is dissolved in water, there are still fine particles that are not completely dissolved, and the embedding rate is 89.7%. The β-carotene microcapsule powder prepared by the technical solution of the present invention has a better embedding rate and better water solubility than the comparative example.

[0085] In summary, the β-carotene microcapsule powder prepared by the technical solution of the present invention has full water solubility and an embedding rate of more than 99%, which is better than that of the control groups 1-2 and 7 in water solubility and embedding rate.

[0086] Test Example 2

[0087] Stability test of β-carotene microcapsule powder

[0088] Test samples: blank group, control group 1-7 and β-carotene microcapsule powder prepared in Example 1-3;

[0089] Test standard: blank group, control group and embodiment. Each group is tested by selecting equal amount of samples. After each group of β-carotene microcapsule powder is dissolved in water, the retention rate of β-carotene microcapsule powder is measured on the 7th day, 14th day, 21st day and 28th day under open light and closed light conditions. Each group of samples is divided into three parallel samples in equal amount, and the average value of the three parallel samples is shown in Table 2.

[0090] Table 2 β-carotene microcapsule powder retention rate

[0091]

[0092] As shown in Table 2, the β-carotene microcapsule powder prepared by the technical solution of the present invention has a higher retention rate than the control example under the conditions of open exposure to light and closed exposure to light as time goes by, and has better stability.

[0093] By comparing Examples 1-3 and Control Group 1-2, it can be seen that the β-carotene microcapsule powder prepared by the technical solution of the present invention has a retention rate of more than 97% on the 7th day under open light, and still has a retention rate of more than 88% on the 28th day, and the retention rate on the 28th day under closed light is more than 95%, while the β-carotene microcapsule powder prepared by Control Group 1-2 has a retention rate of less than 80% on the 28th day under open light, and a retention rate of less than 90% on the 28th day under closed light; the β-carotene microcapsule powder prepared by Example 1-3 has a better retention rate as time goes by under the conditions of open light and closed light than the control group 1-2.

[0094] From the comparison between Example 1 and Control Group 3, it can be seen that, relative to Example 1, Control Group 3 does not add disodium EDTA, that is, does not add an antioxidant synergist. The retention rate of Example 1 on the 28th day after exposure to light is above 88%, and the retention rate on the 28th day after exposure to light is above 95%. The retention rate of Control Group 3 on the 28th day after exposure to light is 80.3%, and the retention rate on the 28th day after exposure to light is 87.2%; the β-carotene microcapsule powder prepared in Example 1 has a better retention rate than that of Control Group 3, that is, the β-carotene microcapsule powder with added antioxidant synergist has a better retention rate than that without added antioxidant synergist.

[0095] From the comparison between Example 1 and Control Groups 4-6, it can be seen that the content of BHA:BHT in Control Group 4 is 8:1 relative to Example 1; the content of BHA:BHT in Control Group 5 is 1:2 relative to Example 1; and BHT is not added in Control Group 6 relative to Example 1. The retention rate of Example 1 on the 28th day after exposure to light is above 88%, and the retention rate on the 28th day after exposure to light is above 95%; the retention rate of Control Group 4 on the 28th day after exposure to light is 80.1%, and the retention rate on the 28th day after exposure to light is 86.5%; the retention rate of Control Group 5 on the 28th day after exposure to light is 80.6%, and the retention rate on the 28th day after exposure to light is 86.6%; the retention rate of Control Group 6 on the 28th day after exposure to light is 80.8%, and the retention rate on the 28th day after exposure to light is 86.9%; the β-carotene microcapsule powder prepared in Example 1 has a better retention rate than Control Groups 4-6.

[0096] From the comparison between Example 1 and Control Group 7, it can be seen that compared with Example 1, Control Group 7 did not add enzymolyzed soybean lecithin, the retention rate of Example 1 on the 28th day after being exposed to light was above 88%, and the retention rate on the 28th day after being exposed to light in a closed state was above 95%, and the retention rate of Control Group 7 on the 28th day after being exposed to light in an open state was 81.7%, and the retention rate on the 28th day after being exposed to light in a closed state was 87.2%; the β-carotene microcapsule powder prepared in Example 1 has a better retention rate than that of Control Group 7.

[0097] In summary, the β-carotene microcapsule powder prepared by the technical solution of the present invention has a higher retention rate than the control example under the conditions of open exposure to light and closed exposure to light as time goes by, and has better stability.

[0098] Test Example 3

[0099] Component Analysis of β-Carotene Microcapsule Powder

[0100] Test samples: blank group, control group 1-7 and β-carotene microcapsule powder prepared in Example 1-3;

[0101] Test standard: Each group of samples was divided into three parallel samples in equal amounts, and the contents of all-trans β-carotene and main isomers in β-carotene microcapsule powder were determined by liquid phase. The average values ​​of the three parallel samples were shown in Table 3.

[0102] Table 3 Content of all-trans β-carotene and its main isomers

[0103] project All-trans / % 9-cis / % 13-cis / % 15-cis / % Blank Group 92.5 0.83 3.15 1.26 Control group 1 91.8 0.89 3.19 1.35 Control group 2 76.8 3.90 12.8 5.31 Control group 3 79.9 3.20 11.6 4.58 Control group 4 79.8 3.15 11.7 4.28 Control group 5 78.9 3.22 11.3 4.45 Control group 6 79.5 3.19 12.0 4.18 Control group 7 92.0 1.05 3.12 1.43 Example 1 92.1 0.95 3.22 1.58 Example 2 91.6 1.03 3.68 1.46 Example 3 91.9 1.11 3.25 1.25

[0104] As shown in Table 3, the all-trans configuration and each cis configuration of the β-carotene microcapsule powder in the control group 1, the control group 7, and the examples 1-3 were almost unchanged compared with the blank group, indicating that the formula processing of the present invention has a good protective effect on β-carotene, prevents the isomerization of β-carotene, and has higher biological effectiveness. Among them, the control group 1 was not subjected to high temperature treatment, and the difference between the control group 7 and the example 1 of the present patent was that no enzymatic soybean lecithin was added.

[0105] Compared with the blank group, the content of all-trans configuration in control group 2-6 was reduced, and the content of cis configuration was increased, wherein the all-trans configuration is the active ingredient of β-carotene that is beneficial to human health. Control group 2 did not fully protect β-carotene; the difference between control group 3 and Example 1 of this patent was that disodium EDTA was not added; the difference between control group 4 and Example 1 of this patent was only that the content of BHA:BHT was 8:1; the difference between control group 5 and Example 1 of this patent was only that the content of BHA:BHT was 1:2; the difference between control group 6 and Example 1 of this patent was only that the amount of BHA added was 1.5 parts, and BHT was not added; compared with Example 1, control groups 2-6 destroyed the synergistic antioxidant effect of BHA+BHT / PG+antioxidant synergist or BHA:BHT was not in the range of 2 to 5:1.

[0106] In summary, the formula and processing steps of the technical solution play a good protective role on β-carotene during high-temperature hot melting, prevent the isomerization of β-carotene, and have higher biological effectiveness.

[0107] The present invention utilizes the synergistic antioxidant effect of BHA+BHT / PG+antioxidant synergist to instantly heat-melt beta-carotene, and then heat-melt it in vegetable oil for the second time through Tween 60 / Tween 80+enzymatic soybean lecithin, which not only prevents the isomerization of beta-carotene during the heating process, has higher biological effectiveness, but also completely dissolves the beta-carotene in the vegetable oil, increasing the stability of the product. In addition, the beta-carotene microcapsule powder prepared by the present invention can have an embedding rate of up to 99%, and is fully water-soluble, which is more conducive to the storage and processing of the product.

[0108] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the technical solution of the present invention, and are not intended to limit the specific implementation methods of the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the claims of the present invention shall be included in the protection scope of the claims of the present invention.

Claims

1. A β-carotene microcapsule powder, characterized in that: The invention comprises the following components in parts by weight: 1-5 parts of beta-carotene, 5-15 parts of vegetable oil, 1.5-3 parts of antioxidant, 2-3 parts of emulsifier, 40-60 parts of wall material, 1-2 parts of stabilizer, 0.5-2 parts of antioxidant synergist and 20-30 parts of water-soluble modified starch.

2. The β-carotene microcapsule powder according to claim 1, characterized in that: The antioxidant is any one of BHA, BHT, TBHQ, PG, natural VE and ascorbic acid or a mixture of several of them.

3. The β-carotene microcapsule powder according to claim 2, characterized in that: The antioxidant is a combination of BHA+BHT, wherein the mass ratio of BHA to BHT is 2-5:1; or, the antioxidant is a combination of BHA+PG, wherein the mass ratio of BHA to PG is 2-5:

1.

4. The β-carotene microcapsule powder according to claim 1, characterized in that: The emulsifier is any one of Tween 60, Tween 80, propylene glycol fatty acid ester, sodium caseinate and enzymatically hydrolyzed soybean lecithin, or a mixture of several of them.

5. The β-carotene microcapsule powder according to claim 4, characterized in that: The emulsifier is a combination of Tween 60 and enzymolyzed soybean lecithin; or, the emulsifier is a combination of Tween 80 and enzymolyzed soybean lecithin.

6. The β-carotene microcapsule powder according to claim 1, characterized in that: The wall material is any one of rice bran protein, lotus seed protein isolate, cyclodextrin, gum arabic and isomaltooligosaccharide or a mixture of several of them; and / or the vegetable oil is any one of soybean oil, corn oil, rapeseed oil, safflower seed oil and peanut oil or a mixture of several of them.

7. The β-carotene microcapsule powder according to claim 1, characterized in that: The stabilizer is any one of sodium pyrophosphate, sodium hexametaphosphate and sodium tripolyphosphate; and / or, the antioxidant synergist is any one of disodium EDTA and citric acid or a mixture of the two; and / or, the water-soluble modified starch is sodium octenyl succinate starch.

8. A method for preparing the β-carotene microcapsule powder according to any one of claims 1 to 8, characterized in that: The preparation process includes: S1, mixing β-carotene, vegetable oil, antioxidant and antioxidant synergist and grinding to obtain a stable β-carotene suspension; S2, heat-dissolving the β-carotene suspension at 160-200°C for 10-40s and then cooling to 60-100°C; S3, adding an emulsifier and performing secondary heat melting for 2 to 10 minutes at 100 to 140° C. to fully dissolve the β-carotene in the vegetable oil to obtain a β-carotene oil solution; S4, mixing the β-carotene oil solution, the wall material and the stabilizer to obtain a β-carotene emulsion; S5. Add water-soluble modified starch to granulate the β-carotene emulsion to obtain β-carotene microcapsule powder.

9. The method according to claim 8, characterized in that In step S1, the particle size of the β-carotene powder after grinding by colloid mill is ≤1 micron.

10. The method according to claim 8, characterized in that In step S4, the β-carotene oil solution, wall material and stabilizer are emulsified and homogenized, and the homogenization pressure is controlled at 60-100 MPa; and / or, in step S5, the β-carotene emulsion is sent to a spray drying granulation device for granulation, and the inlet air temperature of the spray drying granulation device is 100-125°C.

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