Amur corktree bark sunscreen liposome dispersion liquid, preparation method thereof and sunscreen lotion

Abstract

The invention discloses a cortex phellodendri sunscreen liposome dispersion liquid, a preparation method thereof and a sunscreen lotion, and relates to the field of cosmetics. The preparation method of the golden cypress sunscreen liposome dispersion liquid comprises the following steps: performing Soxhlet extraction on golden cypress powder, distilling the obtained extracting solution, and performing freeze drying to obtain golden cypress extract freeze-dried powder; the preparation method comprises the following steps: adding soya bean lecithin, cholesterol and cortex phellodendri extract freeze-dried powder into an organic solvent, carrying out ultrasonic treatment and reduced pressure evaporation to form a lipid membrane, adding water and a surfactant, carrying out rotary hydration, and carrying out ultrasonic treatment in an ice-water bath to obtain the cortex phellodendri sunscreen liposome dispersion liquid. According to the invention, active ingredients of golden cypress are extracted by adopting a Soxhlet extraction method, the golden cypress is prepared into golden cypress sunscreen liposome dispersion liquid as a biological sunscreen agent by adopting a film dispersion method, the preparation raw materials of the golden cypress sunscreen liposome dispersion liquid are simple and easy to obtain, the price is low, and the golden cypress sunscreen liposome dispersion liquid has the characteristics of skin friendliness, mildness, no irritation, greenness, long sunscreen time and good sunscreen effect.

Description

Technical Field

[0001] The invention relates to the field of cosmetics, and in particular to a phellodendron sunscreen liposome dispersion liquid and a preparation method thereof and a sunscreen lotion. Background Art

[0002] Long-term and large amounts of ultraviolet radiation can cause sunburn and photoaging to the body. Sunburn is caused by long-term exposure to ultraviolet rays in the 280-320nm band of sunlight, namely the UVB zone; photoaging is caused by ultraviolet rays in the 320-400nm band, namely the UVA zone. This band has a longer wavelength and a greater ability to penetrate the skin, causing serious damage to the collagen and elastic fibers in the skin, and then damaging the dermis to cause photoaging. In addition to direct damage such as sunburn and photoaging, the indirect damage caused by ultraviolet rays in the UVB and UVA zones to the skin cannot be ignored. Indirect damage refers to the formation of reactive oxygen species, oxidative damage to DNA, and activation of inflammatory cytokines caused by ultraviolet rays in the UVA and UVB zones. These problems will eventually lead to skin damage, suppression of cellular immunity, premature aging, and photocarcinogenesis. Therefore, it is very necessary to effectively protect against ultraviolet rays.

[0003] Sunscreens currently on the market are mainly divided into physical sunscreens and chemical sunscreens. Physical sunscreens refer to sunscreens with minerals that can absorb, reflect and scatter ultraviolet light as the main ingredients, but physical sunscreens have problems such as irritation and clogging pores. In addition, the photocatalytic activity of physical sunscreens cannot be ignored. For example, TiO2 can generate superoxide and hydroxyl radicals (·OH) through sunlight irradiation. These active oxygen species generated by photocatalysis will not only cause the degradation of other sunscreen ingredients in sunscreen, but also react with proteins and DNA molecules on the surface of the skin, causing cancer, enzyme inactivation and other potential biological molecular damage. Chemical sunscreens mainly refer to aromatic compounds that work by absorbing ultraviolet light. Chemical sunscreens have problems such as poor photostability, easy oxidation and deterioration, and skin diseases. In addition, chemical sunscreens such as oxybenzone and octoxybenzoate can also cause coral bleaching and harm the marine ecosystem.

[0004] Since physical sunscreens and chemical sunscreens have the above-mentioned various disadvantages, there is an urgent need to develop a mild, non-irritating, green, and effective sunscreen. Summary of the invention

[0005] Based on the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide a Phellodendron amurense sunscreen liposome dispersion and a preparation method thereof and a sunscreen lotion, aiming to provide a mild, non-irritating, green sunscreen with good sunscreen effect.

[0006] The technical solution of the present invention is as follows:

[0007] The first aspect of the present invention provides a method for preparing a Phellodendron chinense sunscreen liposome dispersion, which comprises the following steps:

[0008] The cortex phellodendri powder is subjected to Soxhlet extraction to obtain an extract;

[0009] The extract is distilled and then freeze-dried to obtain freeze-dried powder of Phellodendron amurense extract;

[0010] Soy lecithin, cholesterol and the freeze-dried powder of the Phellodendron chinense extract are added to an organic solvent, and after ultrasonication, reduced pressure evaporation is performed to form a lipid film, and then water and a surfactant are added. After rotation hydration, ultrasonic treatment is performed in an ice water bath to obtain the Phellodendron chinense sunscreen liposome dispersion (which contains the Phellodendron chinense sunscreen liposome).

[0011] Phellodendron chinense is rich in flavonoids and phenylpropanoid chemical components, among which flavonoids have two main ultraviolet absorption bands in the 200-400nm region, so the Phellodendron chinense sunscreen liposome dispersion has strong ultraviolet absorption ability and the effects of post-sun repair and anti-aging. Phenylpropanoid molecular structure contains phenolic hydroxyl group, which has strong absorption of ultraviolet rays, and small molecule phenylpropanoid compounds also have a good inhibitory effect on tyrosinase activity. In addition, the active ingredients contained in Phellodendron chinense also have antioxidant activity, which can not only avoid the skin photoaging caused by sunlight, but also quench the ·OH produced by chemical sunscreens, and play a role in protecting the skin surface barrier. In addition, Phellodendron chinense also has certain effects in anti-inflammatory, soothing, and barrier repair, and can solve a series of skin problems that occur during sun protection.

[0012] The present invention adopts Soxhlet extraction method to extract effective ingredients from Phellodendron chinense, and uses thin film dispersion method to make Phellodendron chinense sunscreen liposome dispersion as biological sunscreen. That is, the present invention adopts the sunscreen ingredients in natural plant Phellodendron chinense, and uses liposomes for encapsulation. While retaining the above-mentioned effects of the Phellodendron chinense sunscreen ingredients, on the one hand, the biocompatibility of the Phellodendron chinense sunscreen ingredients can be greatly improved, making it more gentle and skin-friendly, reducing irritation to the skin; on the other hand, it can also effectively avoid excessive oxidation of antioxidant ingredients in Phellodendron chinense, which leads to a decrease in sunscreen efficacy, thereby extending the sunscreen time and use time of the Phellodendron chinense sunscreen liposome dispersion, bringing better economic benefits to users. The raw materials for the preparation of the Phellodendron chinense sunscreen liposome dispersion provided by the present invention are simple and easy to obtain, relatively cheap, long sunscreen time and use time, and have the characteristics of skin-friendly, gentle, non-irritating, green and pollution-free, with excellent safety, sunscreen effect, after-sun repair (it also has a certain calming and soothing effect on skin irradiated by ultraviolet rays) and anti-aging effects, which provides a new idea for the safe application of sunscreen agents.

[0013] In the present invention, the extract is distilled to remove the solvent therein. In the present invention, the organic solvent includes but is not limited to ethanol; the water can be purified water; the surfactant can be T-80, i.e., polyoxyethylene (20) sorbitan fatty acid ester, etc., but is not limited thereto. The power used for the ultrasound can be 150 to 500 W (for example, 150 W, 200 W, 250 W, 300 W, 350 W, 400 W, 450 W or 500 W, etc.), and the time used for the ultrasound can be 3 to 10 min (for example, 3 min, 4 min, 5 min, 6 min, 7 min, 8 min, 9 min or 10 min, etc.).

[0014] Optionally, the ratio of soybean lecithin, cholesterol, lyophilized powder of Phellodendron amurense extract, organic solvent, surfactant and water is (5-15) mg: (1-3) mg: (1-5) mg: (1-20) mL: (0.1-2) mg: (1-50) mL. For example, it can be 5 mg: 1 mg: 1 mg: 20 mL: 0.1 mg: 10 mL, 5 mg: 3 mg: 5 mg: 10 mL: 0.2 mg: 20 mL, 10 mg: 2 mg: 1 mg: 1 mL: 0.2 mg: 40 mL, 10 mg: 2 mg: 1 mg: 1 mL: 0.2 mg: 1 mL or 15 mg: 2 mg: 2 mg: 2 mL: 1 mg: 50 mL, etc.

[0015] Optionally, the step of subjecting the Phellodendron chinense powder to Soxhlet extraction to obtain an extract specifically includes: placing the Phellodendron chinense powder into a filter paper tube of a Soxhlet extractor, adding ethanol to a round-bottom flask below the Soxhlet extractor, heating under reflux in a water bath, and obtaining an extract after reaction.

[0016] In this embodiment, the reaction can be stopped after 5 to 10 refluxes. Specifically, the reflux in a water bath until the condensate in the siphon tube is just siphoned down is considered as one reflux.

[0017] In the present invention, the Phellodendron chinense powder is placed in a filter paper cylinder of a Soxhlet extractor, and then an organic solvent such as ethanol is added as a solvent for reflux extraction into a round-bottom flask located at the lower end of the Soxhlet extractor, a condenser is connected to the upper end of the Soxhlet extractor, and then the round-bottom flask is heated in a water bath and refluxed to extract the active ingredients (effective ingredients) in the Phellodendron chinense powder.

[0018] The second aspect of the present invention provides a Phellodendron chinense sunscreen liposome dispersion, wherein the dispersion is prepared by the preparation method of the present invention as described above.

[0019] In the present invention, the Phellodendron chinense sunscreen liposome dispersion prepared by the above preparation method comprises water and Phellodendron chinense sunscreen liposomes dispersed in the water, wherein the Phellodendron chinense sunscreen liposomes are composed of a lyophilized powder of a Phellodendron chinense extract and a liposome layer encapsulating the lyophilized powder of the Phellodendron chinense extract, and the components of the liposome layer include soybean lecithin and cholesterol. Specifically, the water can be purified water.

[0020] The Phellodendron amurense sunscreen liposome dispersion is characterized as a sunscreen agent by being skin-friendly, mild, non-irritating, green and pollution-free, having a good sunscreen effect and a long sunscreen time. The Phellodendron amurense sunscreen liposome also has the effects of post-sun repair and anti-aging.

[0021] The third aspect of the present invention provides a sunscreen lotion, wherein the sunscreen lotion comprises the Phellodendron chinense sunscreen liposome dispersion as described above.

[0022] In the present invention, due to the presence of the Phellodendron amurense sunscreen liposome dispersion, the sunscreen lotion has the characteristics of being skin-friendly, mild, non-irritating, green, having a good sunscreen effect and a long sunscreen time. In addition, the flavonoid glycosides and berberine hydrochloride in the Phellodendron amurense sunscreen liposome also have a certain antibacterial effect, thereby preventing the sunscreen lotion from spoiling and deteriorating.

[0023] Optionally, the sunscreen lotion comprises the following components in parts by weight:

[0024] 1.5-3 parts of ethylhexyl triazone, 2-4 parts of ethylhexyl salicylate, 2-4 parts of squalane, 3-6 parts of butyl methoxydibenzoylmethane, 1.5-3 parts of bis-ethylhexyloxyphenol methoxyphenyl triazine, 6-12 parts of dibutyl adipate, 0.2-0.4 parts of bisabolol, 0.5-1 parts of ammonium acryloyldimethyltaurate / VP copolymer, 3-6 parts of SL1 emulsifier, 1-2 parts of potassium wax phosphate, 1.2-3 parts of cetearyl alcohol, 0.4-1 parts of 1,2-hexanediol, 0.5-2 parts of p-hydroxyacetophenone, 5-10 parts of 1,3-butylene glycol, 30-120 parts of water, 0.6-2 parts of Santing emulsifier G66, 6-10 parts of methylene bis-benzotriazolyl tetramethylbutylphenol, 1-2 parts of Otingmin and 10-40 parts of Phellodendron chinense sunscreen liposome dispersion.

[0025] Among them, ethylhexyl triazone, ethylhexyl salicylate, squalane, butyl methoxydibenzoylmethane, bis-ethylhexyloxyphenol methoxyphenyl triazine, methylene bis-benzotriazolyl tetramethylbutylphenol and phellodendron sunscreen liposome dispersion are used as sunscreen agents, dibutyl adipate, 1,2-hexanediol, 1,3-butylene glycol and water are used as solvents, bisabolol is used as a soothing agent, ammonium acryloyldimethyltaurate / VP copolymer is used as a thickener, Santing emulsifier SL1, potassium cetyl phosphate, cetearyl alcohol and Santing emulsifier G66 are used as emulsifiers, parahydroxyacetophenone is used as a pH adjuster, and Otingmin is used as a preservative.

[0026] In the present invention, the raw materials for preparing the sunscreen lotion are simple and easy to obtain, and the price is relatively low. The sunscreen lotion provided by the present invention is green, healthy, mild, and skin-friendly, can reduce irritation to the skin, has good safety, a long sun protection time, and a good sun protection effect. The sun protection index SPF reaches 46.6, and the PFA reaches 10.9. It has good application prospects and is expected to become a new system of sunscreen lotion on the market, bringing safer and more efficient sun protection options to consumers.

[0027] Optionally, the sunscreen lotion further comprises a sunscreen complex, wherein the sunscreen complex comprises methoxycinnamate and a metal organic framework material coated on the surface of the methoxycinnamate. That is, in the present invention, the sunscreen lotion comprises the phellodendron sunscreen liposome dispersion and the sunscreen complex, which is a dual-effect anti-ultraviolet sunscreen lotion.

[0028] Specifically, the methoxycinnamate may be octyl methoxycinnamate, but is not limited thereto. The metal organic framework may be ZIF-8, but is not limited thereto. ZIF-8 is a metal organic framework synthesized from zinc salt and organic linker 2-methylimidazole, which has the characteristics of high surface area, strong structure, good biocompatibility, large pore size and large pore volume, and also has certain sunscreen effect.

[0029] Octyl methoxycinnamate (OMC for short, its chemical structure is ) is a chemical sunscreen used in more than half of the sunscreen products on the market. OMC will produce active oxygen after exposure to ultraviolet rays, which will damage the skin. Due to the excellent sun protection ability and good user experience of OMC, there is currently no product on the market that can replace OMC. Metal organic framework (MOFs) materials have many excellent properties, including high specific surface area, adjustable pore size and shape, and good biocompatibility. Metal organic framework materials have been used in many fields, such as degradation, hydrogen storage, and medical fields.

[0030] The present invention wraps OMC with a metal organic framework material, and grows a layer of metal organic framework material in situ on the surface of OMC. Due to the porous structure of the metal organic framework material, the active oxygen generated by the OMC after ultraviolet irradiation can be enriched to avoid direct damage to the skin. In addition, the metal organic framework material (such as ZIF-8) has a certain sunscreen effect and a certain ability to shield ultraviolet rays. The metal organic framework material and OMC can be combined to achieve a dual-effect shielding effect of ultraviolet rays, thereby improving the sunscreen ability.

[0031] Specifically, taking the metal organic framework material as ZIF-8 as an example, the sunscreen composite can be prepared by the following method:

[0032] Mixing a first organic solvent (such as methanol, etc.), 2-methylimidazole and OMC to obtain a mixture A;

[0033] Mixing a second organic solvent (such as methanol) and Zn(NO3)2·6H2O to obtain a mixture B;

[0034] The mixture A and the mixture B are mixed, stirred, centrifuged, washed and dried to obtain the sunscreen complex (which can be recorded as OMC / ZIF-8 nanoparticles).

[0035] The present invention adopts an in-situ microcapsule method to prepare nano-microcapsules, that is, OMC is coated with ZIF-8, and a layer of ZIF-8 is in-situ grown on the surface of OMC, which can reduce the irritation and damage of OMC to the skin and enhance its sunscreen effect.

[0036] Optionally, the sunscreen lotion comprises the following components in parts by weight:

[0037] 1.5-3 parts of ethylhexyl triazone, 2-4 parts of ethylhexyl salicylate, 2-4 parts of squalane, 3-6 parts of butyl methoxydibenzoylmethane, 1.5-3 parts of bis-ethylhexyloxyphenol methoxyphenyl triazine, 6-12 parts of dibutyl adipate, 0.2-0.4 parts of bisabolol, 0.1-1 parts of sunscreen complex, 0.5-1 parts of ammonium acryloyldimethyltaurate / VP copolymer, 3-6 parts of cinnamyl emulsifier Agent SL1, 1-2 parts of potassium cetyl phosphate, 1.2-3 parts of cetearyl alcohol, 0.4-1 parts of 1,2-hexanediol, 0.5-2 parts of p-hydroxyacetophenone, 5-10 parts of 1,3-butylene glycol, 30-120 parts of water, 0.6-2 parts of Santing emulsifier G66, 6-10 parts of methylene bis-benzotriazolyl tetramethylbutylphenol, 1-2 parts of Aotingmin and 10-40 parts of Phellodendron chinense sunscreen liposome dispersion.

[0038] Among them, ethylhexyl triazone, ethylhexyl salicylate, squalane, butyl methoxydibenzoylmethane, bis-ethylhexyloxyphenol methoxyphenyl triazine, sunscreen complex, methylene bis-benzotriazolyl tetramethylbutylphenol and phellodendron sunscreen liposome dispersion are used as sunscreen agents, dibutyl adipate, 1,2-hexanediol, 1,3-butylene glycol and water are used as solvents, bisabolol is used as a soothing agent, ammonium acryloyldimethyltaurate / VP copolymer is used as a thickener, Santing emulsifier SL1, 1 part potassium cetyl phosphate, cetearyl alcohol and Santing emulsifier G66 are used as emulsifiers, parahydroxyacetophenone is used as a pH adjuster, and Otingmin is used as a preservative.

[0039] In the present invention, the sunscreen lotion contains Phellodendron chinense sunscreen liposomes, a sunscreen complex composed of a metal organic framework (such as ZIF-8) and OMC (such as OMC / ZIF-8 nanoparticles), and after being applied to the skin surface, Figure 1As shown, the Phellodendron sunscreen liposomes in the sunscreen can absorb ultraviolet rays and play a sunscreen role. At the same time, the Phellodendron liposomes also have the effects of post-sun repair (repairing damaged cells) and delaying aging. At the same time, OMC reflects and absorbs ultraviolet light, plays a sunscreen role, although OMC can produce active oxygen such as OH, but on the one hand, due to the porous structure of the metal organic framework material, the active oxygen produced by OMC after ultraviolet irradiation can be enriched to avoid direct damage to the skin, on the other hand, the Phellodendron sunscreen liposomes have antioxidant function, not only can avoid the skin photoaging phenomenon caused by sunlight irradiation, but also can quench the OH produced by the chemical sunscreen OMC, play a role in protecting the skin surface barrier. Therefore, the synergistic effect of the Phellodendron sunscreen liposomes and the sunscreen complex improves the sunscreen effect, repairs sunburn damage, and avoids OMC from producing active oxygen after ultraviolet irradiation and damaging the skin, so that the sunscreen provided by the present invention covers the full-band ultraviolet sunscreen, long sunscreen time, and skin-friendly, mild, non-irritating, green and pollution-free (while ordinary sunscreens have the disadvantages of limited anti-ultraviolet wavelength, short sunscreen time, and high skin irritation).

[0040] A fourth aspect of the present invention provides a method for preparing a sunscreen lotion, which comprises the following steps, in parts by mass:

[0041] 1.5-3 parts of ethylhexyl triazone, 2-4 parts of ethylhexyl salicylate, 2-4 parts of squalane, 3-6 parts of butyl methoxydibenzoylmethane, 1.5-3 parts of bis-ethylhexyloxyphenol methoxyphenyl triazine, 6-12 parts of dibutyl adipate, and 0.2-0.4 parts of bisabolol are mixed and heated to obtain a first mixture;

[0042] 0.5-1 part of ammonium acryloyldimethyltaurate / VP copolymer, 3-6 parts of Santing emulsifier SL1, 1-2 parts of potassium cetyl phosphate, 1.2-3 parts of cetearyl alcohol, 0.4-1 part of 1,2-hexanediol, 0.5-2 parts of p-hydroxyacetophenone, 5-10 parts of 1,3-butylene glycol and 20-100 parts of water are mixed and heated to obtain a second mixture;

[0043] Mix and disperse 6 to 10 parts of methylene bis-benzotriazolyl tetramethylbutylphenol and 10 to 20 parts of water to obtain a third mixture;

[0044] After adding the first mixture to the second mixture, add 0.6 to 2 parts of the Senting emulsifier G66, and after homogenization, add the third mixture, and after homogenization, add 1 to 2 parts of Aotingmin and 10 to 40 parts of Huangbo sunscreen liposome dispersion, and after stirring evenly, the sunscreen lotion is obtained.

[0045] The preparation method provided by the invention is simple, and the prepared sunscreen lotion is mild, non-irritating, has a fine and smooth paste, good stability, moderate pH, good moisturizing effect (covering sunscreen of the entire ultraviolet band), good sunscreen effect, and long sunscreen time.

[0046] A fifth aspect of the present invention provides a method for preparing a sunscreen lotion, which comprises the following steps, calculated by weight:

[0047] 1.5-3 parts of ethylhexyl triazone, 2-4 parts of ethylhexyl salicylate, 2-4 parts of squalane, 3-6 parts of butyl methoxydibenzoylmethane, 1.5-3 parts of bis-ethylhexyloxyphenol methoxyphenyl triazine, 6-12 parts of dibutyl adipate, 0.2-0.4 parts of bisabolol, and 0.1-1 parts of a sunscreen complex are mixed and heated to obtain a fourth mixture;

[0048] 0.5-1 part of ammonium acryloyldimethyltaurate / VP copolymer, 3-6 parts of Santing emulsifier SL1, 1-2 parts of potassium cetyl phosphate, 1.2-3 parts of cetearyl alcohol, 0.4-1 part of 1,2-hexanediol, 0.5-2 parts of p-hydroxyacetophenone, 5-10 parts of 1,3-butylene glycol and 20-100 parts of water are mixed and heated to obtain a fifth mixture;

[0049] Mix and disperse 6 to 10 parts of methylene bis-benzotriazolyl tetramethylbutylphenol and 10 to 20 parts of water to obtain a sixth mixture;

[0050] After adding the fourth mixture to the fifth mixture, 0.6 to 2 parts of the Senting emulsifier G66 are then added, and after homogenization, the sixth mixture is added, and after homogenization, 1 to 2 parts of Aotingmin and 10 to 40 parts of Huangbo sunscreen liposome dispersion are added, and after stirring evenly, the sunscreen lotion is obtained.

[0051] The preparation method provided by the invention is simple, and the prepared sunscreen lotion is mild, non-irritating, has a fine and smooth paste, good stability, moderate pH, good moisturizing effect, good sunscreen effect (covering sunscreen of the entire ultraviolet band), and has a long sunscreen time.

[0052] Beneficial effects: The present invention adopts the Soxhlet extraction method to extract the effective ingredients from Phellodendron chinense, and uses the thin film dispersion method to prepare it into a Phellodendron chinense sunscreen liposome dispersion as a biological sunscreen. That is, the present invention adopts the sunscreen ingredients in the natural plant Phellodendron chinense, and uses liposomes to encapsulate it. While retaining the efficacy of the Phellodendron chinense sunscreen ingredients, on the one hand, it can greatly improve the biocompatibility of the Phellodendron chinense sunscreen ingredients, making it more gentle and skin-friendly, and reducing irritation to the skin; on the other hand, it can also effectively avoid excessive oxidation of the antioxidant ingredients in Phellodendron chinense, which leads to a decrease in the sunscreen efficacy, thereby extending the sunscreen time and use time of the Phellodendron chinense sunscreen liposomes, bringing better economic benefits to users. The raw materials for the preparation of the Phellodendron chinense sunscreen liposome dispersion provided by the present invention are simple and easy to obtain, relatively cheap, long in use, and have the characteristics of skin-friendly, mild, non-irritating, and green. It has excellent safety, sunscreen effect, and after-sun repair and anti-aging effects, providing a new idea for the safe application of sunscreens. BRIEF DESCRIPTION OF THE DRAWINGS

[0053] Figure 1 Schematic diagram of the sun protection mechanism of the sunscreen lotion containing Phellodendron chinense sunscreen liposomes and OMC / ZIF-8 nanoparticles.

[0054] Figure 2 This is a schematic diagram of the preparation process of Phellodendron chinense sunscreen liposome dispersion.

[0055] Figure 3 These are microscope images of the Phellodendron amurense sunscreen liposomes in the Phellodendron amurense sunscreen liposome dispersion prepared in Example 1, wherein (a) is a low-power microscope image and (b) is a high-power microscope image.

[0056] Figure 4 This is a particle size distribution diagram of the Phellodendron chinense sunscreen liposome dispersion in the Phellodendron chinense sunscreen liposome dispersion prepared in Example 1.

[0057] Figure 5 This is a graph showing the DPPH radical scavenging ability test results of vitamin C, Phellodendron amurense extract freeze-dried powder, Phellodendron amurense sunscreen liposomes and Phellodendron amurense sunscreen liposomes after demulsification.

[0058] Figure 6 This is the infrared spectrum of the OMC / ZIF-8 nanoparticles prepared in Example 3.

[0059] Figure 7 The SEM images of the OMC / ZIF-8 nanoparticles prepared in Example 3 at different magnifications, where (a) is a low magnification and (b) is a high magnification.

[0060] Figure 8TEM images of the OMC / ZIF-8 nanoparticles prepared in Example 3 at different magnifications, where (a) is magnified 25,000 times, (b) is magnified 50,000 times, and (c) is magnified 100,000 times.

[0061] Fig. 9 This is a microscope image of the sunscreen lotion prepared in Example 3.

[0062] Fig.10 The figures are the results of the high temperature stability test of the sunscreen lotion prepared in Example 3, wherein (a) is a physical picture of the sunscreen lotion before the test, (b) is a physical picture of the sunscreen lotion placed in a 40°C oven for the test, and (c) is a physical picture of the sunscreen lotion after the test.

[0063] Fig.11 The figures are graphs showing the low-temperature stability test results of the sunscreen lotion prepared in Example 3, wherein (a) is a physical picture of the sunscreen lotion before the test, (b) is a physical picture of the sunscreen lotion placed in a -20°C oven for the test, and (c) is a physical picture of the sunscreen lotion after the test.

[0064] Fig.12 Graphs showing the centrifugation test results of the sunscreen lotion prepared in Example 3, wherein (a) is a physical picture of the sunscreen lotion before centrifugation, and (b) is a physical picture of the sunscreen lotion after centrifugation.

[0065] Fig.13 The figures are the results of moisturizing tests on different sunscreens, wherein (a) is a physical picture of the skin after wiping the sunscreen prepared in Example 3, (b) is a physical picture of the skin before wiping the sunscreen prepared in Example 3, (c) is a physical picture of the skin after wiping commercially available sunscreen A, (d) is a physical picture of the skin before wiping commercially available sunscreen A, (e) is a physical picture of the skin after wiping commercially available sunscreen B, (f) is a physical picture of the skin before wiping commercially available sunscreen B, (g) is a physical picture of the skin after wiping commercially available sunscreen C, and (h) is a physical picture of the skin before wiping commercially available sunscreen C.

[0066] Fig.14 The graphs are absorbance test results of different sunscreens, where (a) is the sunscreen in Example 3, (b) is commercially available sunscreen A, (c) is commercially available sunscreen B, and (d) is commercially available sunscreen C.

[0067] Fig.15 This is a bar chart showing the sensory evaluation scores of different sunscreens. DETAILED DESCRIPTION

[0068] The present invention provides a Phellodendron chinense sunscreen liposome dispersion and a preparation method thereof and a sunscreen lotion. To make the purpose, technical scheme and effect of the present invention clearer and more specific, the present invention is further described in detail below. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0069] Unless otherwise defined, all technical terms and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used in the specification of the present invention herein are only for the purpose of describing specific embodiments and are not intended to limit the present invention.

[0070] The present invention will be further described below by means of specific examples.

[0071] Unless otherwise specified, the raw materials and instruments used in the following examples are all commercially available products. Information on some of the raw materials is as follows:

[0072] Ammonium acryloyldimethyltaurate / VP copolymer was purchased from Shandong Yousuo Chemical; Senting emulsifier SL1 was purchased from Senting, France; Senting emulsifier G66 was purchased from Senting, France; Otingmin was purchased from Macklin Biochemical Technology.

[0073] The information of some instruments is as follows:

[0074] The ultrasonic cleaning machine (used for ultrasonic treatment) was purchased from Shanghai Kedao Ultrasonic Instrument Co., Ltd., model SK1200GT.

[0075] Example 1

[0076] This embodiment provides a method for preparing a Phellodendron chinense sunscreen liposome dispersion, such as Figure 2 As shown, the following steps are included:

[0077] (1) Preparation of freeze-dried powder of Phellodendron chinense extract

[0078] The Phellodendron amurense medicinal material was placed in an oven for drying, taken out, ground with a mortar, and passed through a 40-mesh sieve to obtain Phellodendron amurense powder.

[0079] Weigh 10 g of Phellodendron chinense powder and put it into a 150 mL filter paper tube of a Soxhlet extractor. Add 90 mL of ethanol into a round-bottom flask, heat and reflux in a water bath for 5 times (one reflux is when the condensate in the siphon tube is just siphoned down). Stop the reaction to obtain an extract.

[0080] After cooling slightly, the device was changed to a distillation device to evaporate a large amount of ethanol in the extract, and then freeze-dried at -20°C overnight to obtain freeze-dried powder of Phellodendron amurense extract.

[0081] (2) Preparation of Phellodendron amurense sunscreen liposome dispersion 500 mg of soybean lecithin, 100 mg of cholesterol and 50 mg of Phellodendron amurense extract freeze-dried powder were weighed and added to 50 mL of anhydrous ethanol, mixed evenly and dispersed by ultrasonication. Then transferred to a 250 mL round-bottom flask and evaporated under reduced pressure at 35°C for 10 min on a rotary evaporator to remove ethanol; after the lipid film was formed, 50 mL of purified water and 100 mg of T-80 were added, and then rotated and hydrated at 40°C for 20 min. After it was fully hydrated, it was ultrasonically treated in an ice water bath (ultrasonic time was 3 min, ultrasonic power was 210 W) to obtain Phellodendron amurense sunscreen liposome dispersion (containing Phellodendron amurense sunscreen liposome).

[0082] The following tests were performed on the Phellodendron chinense sunscreen liposome dispersion prepared in Example 1:

[0083] (1) Determination of encapsulation efficiency

[0084] Take 2 mL of Phellodendron chinense sunscreen liposome dispersion, centrifuge for 30 min at a temperature of 4°C and a centrifuge speed of 12000 r / min, take 1 mL of the supernatant after centrifugation, add anhydrous ethanol to break the emulsion, then make up to 10 mL with anhydrous ethanol, and measure the average absorbance (A1) at a wavelength of 280-320 nm in an ultraviolet spectrophotometer; take another 1 mL of Phellodendron chinense sunscreen liposome dispersion, directly make up to 10 mL with anhydrous ethanol, and similarly measure the average absorbance (A2) at a wavelength of 280-320 nm.

[0085] The encapsulation efficiency was calculated using the following formula (1).

[0086]

[0087] The test results show that the average value of the ultraviolet absorbance before liposome demulsification is A2=1.181, and the average value of the ultraviolet absorbance after liposome demulsification is A1=2.392. According to the above encapsulation rate calculation formula, it can be calculated that the encapsulation rate of the prepared Phellodendron chinense sunscreen liposome is 50.63%, which has a good encapsulation rate and also verifies the successful preparation of the Phellodendron chinense sunscreen liposome.

[0088] (2) Observation of the appearance of Phellodendron chinense sunscreen liposomes under a microscope

[0089] The dispersion of Phellodendron chinense sunscreen liposomes can be observed under a low-power microscope. Figure 3 The image shown in (a) can be observed under a high-power microscope. Figure 3 The image shown in (b) is Figure 3 From (a) in the figure, it can be seen that the Phellodendron amurense sunscreen liposomes in the Phellodendron amurense sunscreen liposome dispersion prepared in this example have a round and full shape and uniform size; Figure 3In (b), it can be observed that the Phellodendron amurense sunscreen liposomes in the Phellodendron amurense sunscreen liposome dispersion contain the contents, i.e., the freeze-dried powder of Phellodendron amurense extract, and the lipid membrane of the Phellodendron amurense sunscreen liposomes is relatively complete, which can encapsulate more Phellodendron amurense sunscreen ingredients. At the same time, the thinner lipid membrane also has higher biocompatibility, which makes the Phellodendron amurense sunscreen ingredients encapsulated in the Phellodendron amurense sunscreen liposomes highly utilized, thereby improving its sunscreen ability.

[0090] (3) Determination of particle size and zeta potential

[0091] Take an appropriate amount of Phellodendron chinense sunscreen liposome dispersion, dilute it with distilled water, mix it evenly, and then use a laser particle size analyzer to determine its particle size and Zeta potential.

[0092] The results are as follows Figure 4 As shown, it can be seen that the particle size of the Phellodendron chinense sunscreen liposomes in the Phellodendron chinense sunscreen liposome dispersion prepared in this example is uniform, the particle size distribution range is relatively concentrated, and the average particle size is about 127nm; the particle size of most of the Phellodendron chinense sunscreen liposomes is about 100nm, which is a small unilamellar liposome. In addition, the Zate potential results show that the Zate potential is low, indicating that the Phellodendron chinense sunscreen liposome dispersion system prepared in this example is relatively stable, and the liposomes are not easy to coagulate and aggregate with each other, and the single liposome is relatively stable and not easy to break.

[0093] (4) DPPH free radical scavenging ability test

[0094] a. Preparation of unemulsified Phellodendron sunscreen liposome sample solution

[0095] The above-prepared Phellodendron sunscreen liposome dispersion (concentration of 1 mg / mL) was diluted by half to obtain a Phellodendron sunscreen liposome dispersion with a concentration of 0.5 mg / mL, that is, each milliliter of Phellodendron sunscreen liposome dispersion contains 0.5 mg of Phellodendron extract freeze-dried powder encapsulated by Phellodendron sunscreen liposomes (the ratio of the mass of the Phellodendron extract freeze-dried powder added in the preparation step (2) of the Phellodendron sunscreen liposome dispersion to purified water is the concentration), that is, the concentration of the Phellodendron sunscreen liposome dispersion is based on the concentration of the Phellodendron extract freeze-dried powder encapsulated by Phellodendron sunscreen liposomes, and the concentration of the Phellodendron sunscreen liposome dispersion in this DPPH free radical scavenging ability test is based on the concentration of the Phellodendron extract freeze-dried powder encapsulated by Phellodendron sunscreen liposomes, and then diluted with water in a gradient manner to 0.4 mg / mL, 0.3 mg / mL, 0.2 mg / mL, and 0.1 mg / mL, respectively, to obtain 5 different concentrations for testing.

[0096] b. Preparation of emulsified Phellodendron chinense sunscreen liposome sample solution

[0097] Take 2 mL of the prepared Phellodendron chinense sunscreen liposome dispersion, centrifuge for 30 min at 4°C and 12000 r / min, take 1 mL of the supernatant after centrifugation, add anhydrous ethanol solution to break the emulsion, and make up to 5 different concentrations of 0.5 mg / mL, 0.4 mg / mL, 0.3 mg / mL, 0.2 mg / mL, and 0.1 mg / mL for testing.

[0098] c. Preparation of Phellodendron chinense freeze-dried powder sample solution

[0099] The lyophilized powder of Phellodendron amurense extract was added into anhydrous ethanol to prepare 5 lyophilized powder sample solutions of Phellodendron amurense with different concentrations of 0.5 mg / mL, 0.4 mg / mL, 0.3 mg / mL, 0.2 mg / mL and 0.1 mg / mL respectively.

[0100] d. Preparation of DPPH solution

[0101] Take an appropriate amount of DPPH (diacylphosphine), dissolve it in anhydrous ethanol and make up to 100 mL to prepare a 40 mmol / L DPPH solution, and refrigerate it for later use.

[0102] e. Preparation of positive control (vitamin C solution)

[0103] Take 5 mg of vitamin C powder, dissolve it in anhydrous ethanol and make up to 10 mL to prepare a 0.5 mg / mL standard solution, which is then diluted to 0.4 mg / mL, 0.3 mg / mL, 0.2 mg / mL, and 0.1 mg / mL, respectively, to obtain 5 different concentrations for testing.

[0104] f. Determination of DPPH free radical scavenging ability

[0105] Add 2 mL of DPPH solution to the quartz cuvette, and then add 2 mL of sample solution. Add 2 mL of DPPH solution and 2 mL of anhydrous ethanol to the blank control well of the sample. The positive control is the same as the sample. Add 2 mL of DPPH solution and 2 mL of anhydrous ethanol to the other quartz cuvettes without sample, and mix the above solutions evenly. After 30 minutes at room temperature and away from light, measure the absorbance at a wavelength of 517 nm every 10 minutes, and take the average value of 3 parallel measurements. Calculate the clearance rate using the following formula (2).

[0106]

[0107] Wherein: Ac is the absorbance of 2 mL of DPPH solution + 2 mL of distilled water; Ai is the absorbance of 2 mL of DPPH solution + 2 mL of sample solution; Aj is the absorbance of 2 mL of sample solution + 2 mL of anhydrous ethanol.

[0108] The results are as follows Figure 5 As shown, it can be seen that the freeze-dried powder of Phellodendron chinense extract (i.e. Figure 5 The DPPH radical scavenging rates of Phellodendron chinense lyophilized powder), vitamin C, Phellodendron chinense sunscreen liposomes, and Phellodendron chinense sunscreen liposomes after demulsification all increased with the increase of their mass concentrations, and were positively correlated with the mass concentration. When the mass concentration was 0.5 mg / mL, the scavenging rates of the four samples all reached the maximum, with scavenging rates of 90.5%, 90.7%, 79.4%, and 88.7%, respectively. In addition, the free radical scavenging rate of Phellodendron chinense sunscreen liposomes was weaker than that of Phellodendron chinense extract lyophilized powder, indicating that the Phellodendron chinense sunscreen liposomes can well wrap up the sunscreen substances in Phellodendron chinense, avoiding the excessive oxidation of the Phellodendron chinense sunscreen substances and causing a decrease in their efficacy. After the Phellodendron chinense sunscreen liposomes were demulsified, their DPPH radical scavenging rate was better than that of the Phellodendron chinense sunscreen liposomes without demulsification, and was close to the DPPH radical scavenging rate of the Phellodendron chinense extract lyophilized powder, indicating that the form of liposome encapsulation does not affect the antioxidant components in Phellodendron chinense.

[0109] Example 2

[0110] This embodiment provides a method for preparing a sunscreen lotion, comprising the following steps:

[0111] Weigh the raw materials according to the raw materials and their proportions in Table 1, mix the components of phase A, heat to 80°C and stir evenly to obtain a phase A mixture, mix the components of phase B, heat to 80°C and stir evenly to obtain a phase B mixture;

[0112] Add the phase A mixture to the phase B mixture, then add the phase C component, homogenize for three minutes, then add the phase D component (obtained by dispersing methylene bis-benzotriazolyltetramethylbutylphenol with water), homogenize for two minutes and stir evenly. When the material is cooled to room temperature, add the phase E component (i.e., Aotingmin and Huangbo sunscreen liposome dispersion) and stir evenly to obtain the sunscreen lotion.

[0113] Table 1, the formula of sunscreen lotion in Example 2

[0114]

[0115] The prepared sunscreen lotion was entrusted to a testing company for testing, and the results showed that all performance properties were good, including uniform appearance without impurities, consistent with the standard, color consistent with the standard, and odor consistent with the standard fragrance; pH was 6.87±0.2, consistent with the standard (4.5~7.5); no stratification after centrifugation (30min, 2500rpm); heat resistance met the standard (kept at 40±1℃ for 72h, and can be used normally after returning to room temperature); cold resistance met the standard (kept at -15~-5℃ for 72h, and can be used normally after returning to room temperature); total bacterial count was less than or equal to 10CFU / g; sun protection index SPF reached 46.6, and PFA reached 10.9.

[0116] On the other hand, most of the sunscreens on the market usually use ethylhexyl methoxycinnamate, phenoxy ketone, etc. as the main chemical sunscreens. However, long-term use of such chemical sunscreens will bring many hazards, such as possibly hindering the normal function of neurons and easily causing adverse symptoms such as allergic dermatitis. Among them, phenoxy ketone substances will cause serious damage to the marine ecology and bring a heavy burden to the ecological environment. Based on these common shortcomings of sunscreens on the market, this embodiment innovatively uses the sunscreen ingredients in the natural plant Phellodendron chinense to avoid the hazards caused by chemical sunscreens, and is prepared into a liposome-encapsulated sunscreen, which can greatly improve the biocompatibility of the sunscreen ingredients in Phellodendron chinense, making it more gentle and skin-friendly, and reducing irritation to the skin; on the other hand, it can also effectively avoid excessive oxidation of antioxidant ingredients in Phellodendron chinense, thereby extending the use time of sunscreen, bringing better economic benefits to users. The sunscreen lotion prepared from this sunscreen has undergone a number of rigorous tests and has achieved good results. Moreover, the raw materials used to prepare this sunscreen are simple and easy to obtain, and the price is relatively low. At the same time, it has excellent sun protection effects. It is expected to become a new system of sunscreen on the market, bringing safer and more efficient sun protection options to consumers.

[0117] Example 3

[0118] This embodiment provides a method for preparing a sunscreen lotion, comprising the following steps:

[0119] Methanol (40 mL), 2-methylimidazole (1298 mg) and OMC (100 mg) were added to a beaker and ultrasonicated until completely dissolved. Methanol (40 mL) and Zn(NO3)2·6H2O (586 mg) were added to another beaker and ultrasonicated until completely dissolved. The contents of the two beakers were mixed and stirred vigorously at room temperature for 1 hour, then centrifuged (12000 rpm, 8 min), washed with methanol 3 times, and dried under vacuum at 60°C to obtain a sunscreen complex, namely OMC / ZIF-8 nanoparticles, whose infrared spectrum (FI-IR) is shown in the figure below. Figure 6 As shown in Figure 2, it can be seen that ZIF successfully encapsulates OMC; the field emission scanning electron microscope (SEM) results are shown in Figure 2 Figure 7 The transmission electron microscope (TEM) image is shown in Figure 8 shown.

[0120] After mixing the components of phase A in Table 2, heating to 80°C and dissolving into a clear and transparent state, a phase A mixture is obtained;

[0121] After mixing the components of phase B in Table 2, heating to 80°C and dissolving into a clear and transparent state, a phase B mixture is obtained;

[0122] The phase A mixture was added to the phase B mixture, and then the phase C components were added and homogenized for three minutes using a homogenizer. The phase D mixture (obtained by dispersing methylene bis-benzotriazolyl tetramethylbutylphenol with water) was added thereto, homogenized for two minutes and stirred evenly. When the material was cooled to room temperature, the phase E mixture (i.e., the Aotingmin and Phellodendron sunscreen liposome dispersion) was added and stirred evenly to obtain a sunscreen lotion.

[0123] Table 2, the formula of sunscreen lotion in Example 3

[0124]

[0125]

[0126] The sunscreen lotion prepared in Example 3 was tested:

[0127] (1) Appearance test

[0128] The sunscreen cream is delicate and smooth. A small amount of sunscreen cream sample is dipped on a glass slide with a glass rod, covered with a cover glass and flattened, and the appearance is observed under a microscope. The results are as follows: Fig. 9 As shown, it can be seen that the Phellodendron amurense sunscreen liposomes are uniform in size and have good morphology.

[0129] (2) pH test

[0130] Weigh 1g sunscreen, add 20mL purified water, heat to a slight boil, stir and cool to obtain a uniform emulsion; insert the electrode into the sample (uniform emulsion), stop stirring after the reading is stable, and read the pH value. Measure 3 times in a row, with an error range of ±0.2, and take the average value. The pH value average of the sunscreen in this embodiment is 6.87 ± 0.2, and the cosmetic pH value is compatible with the pH of the skin under normal circumstances, and will not cause damage to the skin.

[0131] (3) Stability test

[0132] a. High temperature stability: Weigh 5g of sunscreen lotion into a glass test tube and place it in a 40℃ constant temperature box. Take out the test tube after 24 hours, wait until it returns to room temperature, shake the test tube, and observe the state changes of the lotion in the test tube. Fig.10 As shown, the results show that when the sunscreen is taken out after being placed in a 40°C oven for 24 hours, no deterioration such as stratification and precipitation occurs, indicating that the sunscreen has good high-temperature stability.

[0133] b. Low temperature stability: Weigh 5g of sunscreen lotion into a glass test tube and place it in a -20℃ refrigerator. Take out the test tube after 24 hours, wait until it returns to room temperature, shake the test tube, and observe the state changes of the lotion in the test tube. Fig.11As shown, the results show that when the sunscreen is placed in a -20℃ oven for 24 hours and then taken out, it has a delicate appearance without precipitation, separation, discoloration, water seepage, etc., and the glossiness remains the same, indicating that the sunscreen has good low-temperature stability.

[0134] c. Centrifugal test

[0135] Weigh 8g of sunscreen lotion into a 10mL centrifuge tube and centrifuge at 3500r / min for 30min to observe the changes in the state of the lotion. Fig.12 As shown, the results show that after centrifugation at a speed of 3500 r / min for 10 minutes, the physical state of the sunscreen lotion does not change before and after centrifugation, and no stratification or precipitation occurs, which indicates that the sunscreen lotion prepared in this example has good stability.

[0136] (4) Moisturizing test of sunscreen lotion

[0137] At room temperature, a certain amount of the sunscreen lotion of this embodiment, commercial sunscreen lotion A, commercial sunscreen lotion B, and commercial sunscreen lotion C were weighed, and the skin of the same area before and after applying the sunscreen lotion was tested using a skin moisture tester to obtain the moisture content before and after, and compare the moisturizing properties. The results are as follows Fig.13 As shown, the sunscreen provided in this embodiment has a good moisturizing effect (the skin moisture content is 23% before applying the sunscreen, and the skin moisture content is 33% after applying the sunscreen); the moisturizing performance is related to the ratio of the water phase and the oil phase added to the sunscreen. The increase in the proportion of the water phase will improve the skin feel, with good fluidity and no thick feeling; the reduction of the water phase will reduce the fluidity of the sunscreen, bringing a sticky and heavy feeling. The sunscreen provided in this embodiment strikes a balance between moisturizing performance and skin feel, ensuring the user's skin feel when using the sunscreen while maintaining a good moisturizing rate.

[0138] (5) Total colony count test

[0139] Take 12g of lecithin Tween 80 nutrient agar medium, dissolve it in 500mL of boiling distilled water, sterilize it at 121℃ for 20min, and set aside. Take 10mL of sunscreen, add 10mL of paraffin, 10mL of T-80, and 70mL of saline, shake well, and you will get a 1:10 diluted test solution. Inject 1mL of the 1:10 diluted test solution into two sterile plates respectively, and inject another 1mL into a 9mL sterilized saline test tube, mix well, and you will get a 1:100 test solution. Inject 1mL of the 1:100 diluted test solution into two sterile plates respectively, pour the lecithin T-80 nutrient agar medium that has been sterilized and cooled to about 45℃ into the plate to the scale line, rotate the plate to mix it evenly, and after the culture medium is completely solidified, turn the plate over and place it in a 36℃ incubator for 48h. Take another sterilized empty plate without sample and follow the above steps as a blank control group. Observe with a 5x to 10x magnifying glass and count the number of colonies. Record the number of colonies on each plate and calculate the average number of colonies grown on each plate at the same dilution. According to the provisions of the "Technical Specifications for Safety of Cosmetics" (2015 edition) that the total number of colonies is ≤1000CFU / g, check whether the total number of colonies in the sunscreen meets the standard. The number of colonies cultivated meets the provisions of the "Technical Specifications for Safety of Cosmetics" (2015 edition) that the total number of colonies is ≤1000CFU / g, indicating that the number of colonies in the sunscreen prepared in this example is qualified.

[0140] (6) Sunscreen efficacy testing

[0141] a. In vitro instrumental protection effect test

[0142] According to the light industry standard QB / T 2410-1998 "Evaluation Method for Sunscreen Effect of Sunscreen Cosmetics in UVB Zone - Ultraviolet Absorbance Method", the tape method was used to evaluate the ultraviolet protection effect of the sunscreen lotion prepared in this embodiment, three commercially available sunscreen lotions and ordinary lotion products. A rectangular 3M medical breathable tape with an area of ​​1 cm×4 cm was taken and attached to the light-transmitting side of the cuvette. 8 mg of sunscreen lotion was weighed and evenly spread on the tape with a glass rod. The spread sample was stored in the dark at 37°C for 15 min; a 3M medical breathable tape of the same size was taken as a blank control and placed in an ultraviolet spectrophotometer, and the instrument was reset to zero; at this time, the cuvette coated with the sunscreen lotion sample was placed in the instrument, and its ultraviolet absorbance at wavelengths of 280-320 nm (UVB) and 320-400 nm (UVA) was detected respectively, and the test was repeated 3 times and the average value was calculated. For each product, three samples were prepared and the average value was calculated using the same method, which was the UV absorbance of the sample. The comprehensive evaluation table of the sunscreen effect is shown in Table 3.

[0143] Table 3. Comprehensive evaluation table of sun protection effect

[0144]

[0145] The results are as follows Fig.14 As shown, it can be seen that the average absorbance of the sunscreen in this embodiment is 2.308, the average absorbance of the commercial sunscreen A is 2.117, the average absorbance of the commercial sunscreen B is 1.784, and the average absorbance of the commercial sunscreen C is 2.041. Because the sunscreen in this embodiment is composed of the double sunscreen ingredients of Phellodendron sunscreen liposomes and OMC / ZIF-8 nanoparticles, the sunscreen effect is greater than that of most sunscreens on the market. There are sunscreens on the market that specifically protect against some bands of ultraviolet rays, such as the commercial sunscreen C, which has a strong sunscreen effect in the range of 290-330nm, but the sunscreen situation for other bands is greatly reduced. The sunscreen in this embodiment covers the full band of ultraviolet rays, and the sunscreen effect is better than that of commercial sunscreens.

[0146] b. Sensory evaluation of sunscreen lotion

[0147] In the field of cosmetics, sensory evaluation can solve some physiological problems that cannot be solved by physical and chemical instrument analysis. It has the most authentic perception of the human body, so it plays an indispensable role in the research and development and quality management of cosmetics. A questionnaire survey was conducted on campus, and 100 sensory evaluation forms were distributed. A total of 100 students passing by were randomly selected on various sections of the school. A circle of skin with a diameter of about 2 cm was selected on the outside of their dry and clean right arm as the test area. About 2 mg of sunscreen was applied per square centimeter on the test area. The 100 questionnaire data obtained were statistically analyzed using Excel, and the sunscreen sensory evaluation form is shown in Table 4.

[0148] Table 4. Sensory evaluation table of sunscreen lotion

[0149]

[0150]

[0151] The results are as follows Fig.15 As shown, in the five evaluation indicators, the sunscreen lotion in this embodiment has achieved good responses. The light yellow paste is made of the natural plant ingredient Phellodendron chinense; no additional chemical film-forming agent is added, so the film-forming speed is slightly lower than that of common sunscreen lotions on the market; generally speaking, the sensory evaluation of the sunscreen lotion prepared in this embodiment is not much different from that of other sunscreen lotions on the market, and it is superior to other common sunscreen lotions on the market in terms of moisturizing type and other aspects.

[0152] It should be understood that the application of the present invention is not limited to the above examples. For ordinary technicians in this field, improvements or changes can be made based on the above description. All these improvements and changes should fall within the scope of protection of the claims attached to the present invention.

Claims

1. A method for preparing a Phellodendron chinense sunscreen liposome dispersion, characterized in that: The steps include: The cortex phellodendri powder is subjected to Soxhlet extraction to obtain an extract; The extract is distilled and then freeze-dried to obtain freeze-dried powder of Phellodendron amurense extract; Soy lecithin, cholesterol and the freeze-dried powder of the Phellodendron amurense extract are added to an organic solvent, and after ultrasonication, reduced pressure evaporation is performed to form a lipid film, and then water and a surfactant are added. After rotation hydration, ultrasonic treatment is performed in an ice water bath to obtain the Phellodendron amurense sunscreen liposome dispersion.

2. The preparation method according to claim 1, characterized in that: The ratio of soybean lecithin, cholesterol, lyophilized powder of Phellodendron amurense extract, organic solvent, surfactant and water is (5-15) mg: (1-3) mg: (1-5) mg: (1-20) mL: (0.1-2) mg: (1-50) mL.

3. The preparation method according to claim 1, characterized in that: The step of subjecting the cortex phellodendri powder to Soxhlet extraction to obtain an extract specifically comprises: Put the Phellodendron chinense powder into the filter paper tube of the Soxhlet extractor, add ethanol into the round-bottom flask below the Soxhlet extractor, heat under reflux in a water bath, and after reaction, obtain an extract.

4. A Phellodendron chinense sunscreen liposome dispersion, characterized in that: The preparation method is described in any one of claims 1 to 3.

5. A sunscreen lotion, characterized in that: The sunscreen lotion comprises the Phellodendron chinense sunscreen liposome dispersion according to claim 4.

6. The sunscreen lotion according to claim 5, characterized in that: The sunscreen lotion comprises the following components in parts by weight: 1.5-3 parts of ethylhexyl triazone, 2-4 parts of ethylhexyl salicylate, 2-4 parts of squalane, 3-6 parts of butyl methoxydibenzoylmethane, 1.5-3 parts of bis-ethylhexyloxyphenol methoxyphenyl triazine, 6-12 parts of dibutyl adipate, 0.2-0.4 parts of bisabolol, 0.5-1 parts of ammonium acryloyldimethyltaurate / VP copolymer, 3-6 parts of SL1 emulsifier, 1-2 parts of potassium wax phosphate, 1.2-3 parts of cetearyl alcohol, 0.4-1 parts of 1,2-hexanediol, 0.5-2 parts of p-hydroxyacetophenone, 5-10 parts of 1,3-butylene glycol, 30-120 parts of water, 0.6-2 parts of Santing emulsifier G66, 6-10 parts of methylene bis-benzotriazolyl tetramethylbutylphenol, 1-2 parts of Otingmin and 10-40 parts of Phellodendron chinense sunscreen liposome dispersion.

7. The sunscreen lotion according to claim 5, characterized in that: The sunscreen lotion also includes a sunscreen complex, which includes methoxycinnamate and a metal organic framework material coated on the surface of the methoxycinnamate.

8. The sunscreen lotion according to claim 7, characterized in that: The sunscreen lotion comprises the following components in parts by weight: 1.5-3 parts of ethylhexyl triazone, 2-4 parts of ethylhexyl salicylate, 2-4 parts of squalane, 3-6 parts of butyl methoxydibenzoylmethane, 1.5-3 parts of bis-ethylhexyloxyphenol methoxyphenyl triazine, 6-12 parts of dibutyl adipate, 0.2-0.4 parts of bisabolol, 0.1-1 parts of sunscreen complex, 0.5-1 parts of ammonium acryloyldimethyltaurate / VP copolymer, 3-6 parts of cinnamyl emulsifier Agent SL1, 1-2 parts of potassium cetyl phosphate, 1.2-3 parts of cetearyl alcohol, 0.4-1 parts of 1,2-hexanediol, 0.5-2 parts of p-hydroxyacetophenone, 5-10 parts of 1,3-butylene glycol, 30-120 parts of water, 0.6-2 parts of Santing emulsifier G66, 6-10 parts of methylene bis-benzotriazolyl tetramethylbutylphenol, 1-2 parts of Aotingmin and 10-40 parts of Phellodendron chinense sunscreen liposome dispersion.

9. A method for preparing a sunscreen lotion, characterized in that: By weight, the method comprises the following steps: 1.5-3 parts of ethylhexyl triazone, 2-4 parts of ethylhexyl salicylate, 2-4 parts of squalane, 3-6 parts of butyl methoxydibenzoylmethane, 1.5-3 parts of bis-ethylhexyloxyphenol methoxyphenyl triazine, 6-12 parts of dibutyl adipate, and 0.2-0.4 parts of bisabolol are mixed and heated to obtain a first mixture; 0.5-1 part of ammonium acryloyldimethyltaurate / VP copolymer, 3-6 parts of Santing emulsifier SL1, 1-2 parts of potassium cetyl phosphate, 1.2-3 parts of cetearyl alcohol, 0.4-1 part of 1,2-hexanediol, 0.5-2 parts of p-hydroxyacetophenone, 5-10 parts of 1,3-butylene glycol and 20-100 parts of water are mixed and heated to obtain a second mixture; Mix and disperse 6 to 10 parts of methylene bis-benzotriazolyl tetramethylbutylphenol and 10 to 20 parts of water to obtain a third mixture; After adding the first mixture to the second mixture, add 0.6 to 2 parts of the Senting emulsifier G66, and after homogenization, add the third mixture, and after homogenization, add 1 to 2 parts of Aotingmin and 10 to 40 parts of Huangbo sunscreen liposome dispersion, and after stirring evenly, the sunscreen lotion is obtained.

10. A method for preparing a sunscreen lotion, characterized in that: By weight, the method comprises the following steps: 1.5-3 parts of ethylhexyl triazone, 2-4 parts of ethylhexyl salicylate, 2-4 parts of squalane, 3-6 parts of butyl methoxydibenzoylmethane, 1.5-3 parts of bis-ethylhexyloxyphenol methoxyphenyl triazine, 6-12 parts of dibutyl adipate, 0.2-0.4 parts of bisabolol, and 0.1-1 parts of a sunscreen complex are mixed and heated to obtain a fourth mixture; 0.5-1 part of ammonium acryloyldimethyltaurate / VP copolymer, 3-6 parts of Santing emulsifier SL1, 1-2 parts of potassium cetyl phosphate, 1.2-3 parts of cetearyl alcohol, 0.4-1 part of 1,2-hexanediol, 0.5-2 parts of p-hydroxyacetophenone, 5-10 parts of 1,3-butylene glycol and 20-100 parts of water are mixed and heated to obtain a fifth mixture; Mixing 6 to 10 parts of methylene bis-benzotriazolyl tetramethylbutylphenol and 10 to 20 parts of water to obtain a sixth mixture; The fourth mixture is added to the fifth mixture, and then 0.6 to 2 parts of the Senting emulsifier G66 are added. After homogenization, the sixth mixture is added. After homogenization, 1 to 2 parts of Aotingmin and 10 to 40 parts of Huangbo sunscreen liposome dispersion are added. After stirring evenly, the sunscreen lotion is obtained.

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