Pharmaceutical composition for treating diabetic skin wounds as well as preparation method and application of pharmaceutical composition

Abstract

The invention discloses a pharmaceutical composition for treating diabetic skin wounds and a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicine compositions. In order to develop a medicine with a good treatment effect on diabetic skin wounds, according to the theory of traditional Chinese medicine, baked placentas and baked leeches are ground into particles respectively, and the particles are mixed according to a specific proportion to obtain the pharmaceutical composition for treating the diabetic skin wounds. The traditional Chinese medicine is simple in component and has the effects of removing blood stasis, activating blood, breaking blood, removing stasis, astringing sore, promoting tissue regeneration, promoting tissue regeneration and promoting healing. The ointment obtained by mixing the pharmaceutical composition serving as an effective component with vaseline is simple in use method and has a remarkable promoting effect on healing of diabetic skin wounds.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicine compositions, and in particular relates to a medicine composition for treating diabetic skin wounds, and a preparation method and application thereof. Background Art

[0002] Diabetes is a chronic metabolic disease characterized by high blood sugar levels. Long-term exposure to high blood sugar levels can lead to a variety of complications, including cardiovascular disease, neuropathy, nephropathy, and skin lesions. Among them, skin lesions caused by diabetes may cause wounds to be protracted and difficult to heal. These wounds are difficult to heal due to poor blood circulation, increased risk of infection, and decreased healing ability. Diabetic wounds themselves and their complications may bring a series of hazards, which not only affect the patient's quality of life, but may also cause serious health problems, causing great pain and inconvenience to patients.

[0003] Traditional treatments for diabetic skin wounds include debridement, antibiotics, and surgery, but these methods have limited effects on difficult-to-heal wounds. Currently, there are some drugs on the market for the treatment of diabetic wounds, such as growth factors, extracellular matrix products, and bioactive dressings. However, these drugs have problems such as high cost, unstable effects, or side effects. In recent years, researchers have been exploring the use of new treatments to improve the treatment of diabetic wounds.

[0004] Difficult-to-heal wounds in diabetes belong to the category of "sores" in traditional Chinese medicine. According to the theory of yin and yang, it is considered to be a Yin syndrome sore, and the pathogenesis is yang deficiency. According to the theory of traditional Chinese medicine, physiological function is yang, body structure is yin; Qi is yang, and blood is yin. The root cause of sores is blood stasis and loss of skin; "stasis" and "rot" are mutually causal. Traditional Chinese medicine believes that "rot" is the main factor that makes difficult-to-heal ulcers difficult to heal. "If rotten flesh is not removed, new flesh will not grow." The theory of "removing rot and growing new flesh", "simmering pus and growing flesh", and "skin flat and skin long" is proposed. However, it is often difficult to grow flesh after removing rot, and the disease is repeated. According to the theory of qi and blood, the pathogenesis of sores is qi and blood stagnation, that is, "all sores are caused by disharmony of the body's defense system, qi and blood stagnation, and then carbuncle and swelling are born." Wang Ji of the Ming Dynasty recorded in "Surgery Principles": "Carbuncle, sore, and boil are all caused by qi and blood stasis." He advocated qi and blood stasis as the basic pathogenesis and proposed the treatment method of "activating blood circulation and growing flesh." Therefore, if a pharmaceutical composition for treating diabetic wounds can be developed in combination with TCM theory, it will be of great significance for the treatment of refractory diabetic wounds.

[0005] Placenta has the effects of anti-infection, anti-inflammatory, blood stasis removal, sore support, astringency, muscle growth and new growth. It can be used to improve local tissue blood circulation disorders, repair wound tissues, enhance local defense, and treat sores that have been ulcerated for a long time and cannot be healed. Leeches have a mild and lasting effect and are good at breaking up hard masses; they break up blood and remove blood stasis, promote blood circulation and remove blood stasis, improve blood circulation, and promote the repair of damaged skin. However, there are no reports that placenta or leeches can treat diabetic wounds. Summary of the invention

[0006] In order to develop a drug with good therapeutic effect on diabetic skin wounds, the present invention grinds the baked placenta and the baked leech into particles respectively according to the theory of traditional Chinese medicine, and obtains a pharmaceutical composition for treating diabetic skin wounds after mixing them in a specific proportion. The pharmaceutical composition combines the placenta and the leech together, and has the effects of removing blood stasis, supporting ulcers, astringing, and promoting tissue regeneration; breaking blood and removing blood stasis, activating blood circulation and removing blood stasis, and promoting skin repair.

[0007] In order to solve the above technical problems and achieve corresponding technical effects, the present invention specifically provides the following technical solutions:

[0008] The first object of the present invention is to provide a pharmaceutical composition for treating diabetic skin wounds, wherein the pharmaceutical composition is composed of the following raw materials in parts by mass: 20-30 parts of baked placenta particles and 2-3 parts of baked leech particles.

[0009] In one embodiment of the present invention, the mass ratio of the roasted placenta particles to the roasted leech particles in the pharmaceutical composition is 10:1.

[0010] The second object of the present invention is to provide a method for preparing the above-mentioned pharmaceutical composition, comprising the following steps:

[0011] (1) roasting the placenta and grinding it to obtain placenta particles;

[0012] (2) scalding leeches to death with boiling water, roasting them until they turn pale yellow, and grinding them to obtain leech granules;

[0013] (3) The placenta particles obtained in step (1) and the leech particles obtained in step (2) are mixed in a mass ratio of (20-30):(2-3) to obtain a pharmaceutical composition.

[0014] In one embodiment of the present invention, the placenta is a human placenta, preferably a first-born placenta, and secondarily a placenta from a healthy, disease-free woman.

[0015] In one embodiment of the present invention, the particle size of the placenta particles in step (1) is 70-80 μm.

[0016] In one embodiment of the present invention, the particle size of the leech particles in step (2) is 70-80 μm.

[0017] In one embodiment of the present invention, the leeches in step (2) are captured in May-June or September-October.

[0018] In one embodiment of the present invention, leeches of medium to small size are preferably used as medicine, and the leeches are preferably roasted until they are light yellow and crisp.

[0019] The third object of the present invention is to provide the use of the above-mentioned pharmaceutical composition in treating diabetic skin wounds.

[0020] In one embodiment of the present invention, the dosage form of the drug is an ointment preparation for external use.

[0021] The fourth object of the present invention is to provide an ointment for treating diabetic skin wounds, wherein the ointment is prepared by mixing the above-mentioned pharmaceutical composition with vaseline in a mass ratio of 1:(5-10).

[0022] In one embodiment of the present invention, the ointment is prepared by mixing the above-mentioned pharmaceutical composition with vaseline in a mass ratio of 1:10.

[0023] In one embodiment of the present invention, the ointment is used to fill or cover the skin wound, with the thickness of the ointment not exceeding 0.5 cm, and is fixed with a layer of vaseline gauze, and the ointment is changed once every 72 hours.

[0024] The fifth object of the present invention is to provide a method for preparing the above-mentioned ointment, wherein the preparation method is to add the above-mentioned pharmaceutical composition to sterilized vaseline for mixing, and the mass ratio of the pharmaceutical composition to vaseline is 1:(5-10).

[0025] In one embodiment of the present invention, the sterilization treatment may be performed by ultraviolet sterilization, high pressure sterilization or other sterilization methods.

[0026] In one embodiment of the present invention, the sterilization is performed by dry heat sterilization, the dry heat sterilization temperature is 180° C., and the sterilization time is 90 min.

[0027] Beneficial effects of the present invention:

[0028] Traditional Chinese medicine believes that diabetic wounds belong to the category of "sores", and the root cause of sores is blood stasis and skin loss. The present invention takes into account that the vascular endothelium of diabetic patients is damaged and the blood circulation is poor, which is not conducive to the healing of skin wounds. Starting from "activating blood circulation and promoting muscle growth", the placenta with the function of promoting wound healing and enhancing immunity and the leech with the function of breaking hard masses, removing blood stasis, activating blood circulation and removing blood stasis, reducing swelling and relieving pain, and promoting the repair of damaged skin are selected as the main ingredients. The baked placenta and the baked leech are ground into particles respectively, and a pharmaceutical composition for treating diabetic skin wounds is obtained after mixing them in a specific proportion. The pharmaceutical composition combines the placenta and the leech together, has simple ingredients, has the functions of removing blood stasis and activating blood circulation, removing blood stasis, supporting sores and astringing, promoting muscle growth and healing, and can play the role of anti-infection, anti-inflammatory, and enhancing immunity; the preparation method is simple, the placenta and the leech are ground, and the drug can be fully contacted with the wound surface, increasing the efficacy, and can be used for the treatment of diabetic skin wounds.

[0029] In addition, the present invention uses a pharmaceutical composition made of baked placenta particles and baked leech particles as an effective ingredient, and mixes it with vaseline to prepare an ointment for wound treatment of diabetic rat models. The results show that the ointment can effectively promote the healing of diabetic skin wounds and shorten the time of skin healing. The ointment raw material components provided by the present invention do not require complex processing, and the drug source is abundant; the preparation process is simple; the use process is simple and convenient, and the drug can be administered externally until the lesion, which is safe and effective, and the treatment cost is low; it can promote the healing of diabetic wounds, and can be used for skin and muscle tissue defects caused by various traumas. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 The blood glucose test results of rats in each group before STZ injection and on days 3, 7, 14, and 21 after STZ injection; *P<0.05 compared with the normal group;

[0031] Figure 2 The weight test results of rats in each group before STZ injection and on days 3, 7, 14 and 21 after STZ injection; *P<0.05 compared with the normal group;

[0032] Figure 3 The wound area measurement results of rats in each group on the 7th, 14th and 21st days after medication; *P<0.05 compared with the normal group, #P<0.05 compared with the model group. DETAILED DESCRIPTION

[0033] The present invention is further described in detail below in conjunction with specific examples and accompanying drawings. The following examples are convenient for better understanding of the present invention, but are not intended to limit the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified. The drugs, reagents, and materials used in the following examples can all be purchased through commercial channels unless otherwise specified.

[0034] The baked placenta used in the present invention can be directly purchased through regular commercial channels.

[0035] Example 1: A pharmaceutical composition for treating diabetic skin wounds

[0036] (1) Preparation of placental particles

[0037] The baked placenta was purchased and ground to obtain placenta particles with a particle size of 70-80 μm.

[0038] (2) Preparation of leech particles

[0039] Leeches are captured in May to June and September to October, scalded to death with boiling water, placed in bamboo containers, and washed with running water overnight (12 hours) at room temperature; after washing the leeches, they are placed in a ventilated place to allow the water to seep out and evaporate; the dried leeches are placed in a casserole (or tile), and the casserole (or tile) is placed on a rack (about 20 cm away from the fire source), the fire source is always kept at a low heat, the baking temperature is about 50 to 60°C, and they are slowly baked to dry evenly on both sides, and the baking time is about 8-10 hours. The leeches are light yellow and crisp; after baking, they are placed in a ventilated place and cooled to room temperature; after grinding, leech particles with a particle size of 70-80 μm are obtained.

[0040] (3) Preparation of a pharmaceutical composition for treating diabetic skin wounds

[0041] Weigh 20 g of the placenta particles obtained in step (1) and 3 g of the leech particles obtained in step (2), and mix them to obtain a pharmaceutical composition for treating diabetic skin wounds.

[0042] Example 2: A pharmaceutical composition for treating diabetic skin wounds

[0043] (1) Preparation of placental particles

[0044] The baked placenta was purchased and ground to obtain placenta particles with a particle size of 70-80 μm.

[0045] (2) Preparation of leech particles

[0046] Leeches are captured in May to June and September to October, scalded to death with boiling water, placed in bamboo containers, and washed with running water overnight (12 hours) at room temperature; after washing the leeches, they are placed in a ventilated place to allow the water to seep out and evaporate; after drying, the leeches are placed in a casserole (or tile), and the casserole (or tile) is placed on a rack (about 20 cm away from the fire source), the fire source is always kept at a low heat, the baking temperature is about 50 to 60°C, and they are slowly baked, and both sides are evenly baked for about 8-10 hours. The leeches are light yellow and crisp; after baking, they are placed in a ventilated place and cooled at room temperature; after grinding, leech particles with a particle size of 70-80μm are obtained.

[0047] (3) Preparation of a pharmaceutical composition for treating diabetic skin wounds

[0048] Weigh 30 g of the placenta particles obtained in step (1) and 3 g of the leech particles obtained in step (2), and mix them to obtain a pharmaceutical composition for treating diabetic skin wounds.

[0049] Example 3: A pharmaceutical composition for treating diabetic skin wounds

[0050] (1) Preparation of placental particles

[0051] The baked placenta was purchased and ground to obtain placenta particles with a particle size of 70-80 μm.

[0052] (2) Preparation of leech particles

[0053] Leeches are captured in May to June and September to October, scalded to death with boiling water, placed in bamboo containers, and washed with running water overnight (12 hours) at room temperature; after washing the leeches, they are placed in a ventilated place to allow the water to seep out and evaporate; after drying, the leeches are placed in a casserole (or tile), and the casserole (or tile) is placed on a rack (about 20 cm away from the fire source), the fire source is always kept at a low heat, the baking temperature is about 50 to 60°C, and they are slowly baked, and both sides are evenly baked for about 8-10 hours. The leeches are light yellow and crisp; after baking, they are placed in a ventilated place and cooled at room temperature; after grinding, leech particles with a particle size of 70-80μm are obtained.

[0054] (3) Preparation of a pharmaceutical composition for treating diabetic skin wounds

[0055] Weigh 30 g of the placenta particles obtained in step (1) and 2 g of the leech particles obtained in step (2), and mix them to obtain a pharmaceutical composition for treating diabetic skin wounds.

[0056] Example 4: An ointment for treating diabetic skin wounds containing the pharmaceutical composition described in Example 1

[0057] Vaseline was sterilized by dry heat at 180°C for 90 minutes. The sterilized vaseline was filtered and cooled to 40-60°C, and the pharmaceutical composition obtained in Example 1 was added to the vaseline at a mass ratio of the pharmaceutical composition to vaseline of 1:10, and the ointment for treating diabetic skin wounds was obtained after mixing.

[0058] Example 5: An ointment for treating diabetic skin wounds containing the pharmaceutical composition described in Example 2

[0059] Vaseline was sterilized by dry heat at 180°C for 90 minutes. The sterilized vaseline was filtered and cooled to 40-60°C, and the pharmaceutical composition obtained in Example 2 was added to the vaseline at a mass ratio of the pharmaceutical composition to vaseline of 1:10, and the ointment for treating diabetic skin wounds was obtained after mixing.

[0060] Example 6: An ointment for treating diabetic skin wounds containing the pharmaceutical composition described in Example 3

[0061] Vaseline was sterilized by dry heat at 180°C for 90 minutes. The sterilized vaseline was filtered and cooled to 40-60°C, and the pharmaceutical composition obtained in Example 3 was added to the vaseline at a mass ratio of 1:10 to the vaseline. The ointment for treating diabetic skin wounds was obtained after mixing.

[0062] Comparative Example 1: An ointment containing only placenta particles

[0063] Purchase baked placenta, grind it to obtain placenta particles with a particle size of 70-80 μm. Dry heat sterilize vaseline at a temperature of 180°C for 90 minutes. Filter and sterilize the vaseline, cool it to 40-60°C, add placenta particles to the vaseline at a mass ratio of 1:10, and mix well to obtain an ointment containing only placenta particles.

[0064] Comparative Example 2: An ointment containing only leech particles

[0065] Leeches are caught in May-June and September-October, scalded to death with boiling water, placed in bamboo containers, and washed overnight (12 hours) under running water at room temperature; after washing the leeches, they are placed in a ventilated place to allow water to seep out and evaporate; after drying, the leeches are placed in a casserole (or tile), which is placed on a stand (about 20 cm away from the fire source), and the fire source is always kept at a low fire, and the baking temperature is about 50 to 60°C, and they are slowly baked and dried evenly on both sides. The baking time is about 8-10 hours, and the leeches are light yellow and crisp; after drying, they are placed in a ventilated place and cooled at room temperature; after grinding, leech particles with a particle size of 70-80μm are obtained. Vaseline is dry-heat sterilized at a temperature of 180°C and a sterilization time of 90 minutes. The vaseline after filtration and sterilization is cooled to 40-60° C., and the leech particles are added to the vaseline at a mass ratio of leech particles to vaseline of 1:10. After mixing, an ointment containing only leech particles is obtained.

[0066] Comparative Example 3: An ointment containing placenta particles and leech particles

[0067] Purchase baked placenta, grind it to obtain placenta particles with a particle size of 70-80 μm. Capture leeches in May-June and September-October, scald them to death with boiling water, place them in bamboo containers, and wash them with running water overnight (12 hours) at room temperature; after washing the leeches, place them in a ventilated place to allow water to seep out and evaporate; place the dried leeches in a casserole (or tile), place the casserole (or tile) on a bracket (about 20 cm away from a fire source), keep the fire source at a low temperature, bake at a temperature of about 50 to 60°C, slowly bake, and evenly bake both sides for about 8-10 hours. The leeches are light yellow and crisp; after baking, place them in a ventilated place and cool at room temperature; grind them to obtain leech particles with a particle size of 70-80 μm. Mix the placenta particles and leech particles in a mass ratio of 1:1 to form a pharmaceutical composition. Vaseline is sterilized by dry heat at a temperature of 180° C. for 90 minutes. The sterilized vaseline is filtered and cooled to 40-60° C., and the drug composition is added to the vaseline at a mass ratio of 1:10 to the vaseline, and the ointment containing the placenta particles and the leech particles is obtained after mixing.

[0068] The present invention verifies the therapeutic effect of the ointments prepared in Comparative Examples 1-3 and Examples 4-6 in treating diabetic skin wounds through animal experiments, and the specific method is as follows:

[0069] The ointments prepared in Comparative Examples 1-3 and Examples 4-6 were respectively recorded as Drug 1, Drug 2, Drug 3, Drug 4, Drug 5 and Drug 6, and the control drug was vaseline sterilized by dry heat at 180° C. for 90 min.

[0070] 80 male SD rats of 6 weeks old and weighing 240-250g were selected and adaptively fed for 7 days. They were randomly divided into normal group, model group, drug group 1, drug group 2, drug group 3, drug group 4, drug group 5 and drug group 6, with 10 rats in each group. Except for the normal group, rats in other groups were intraperitoneally injected with 1% STZ (50mg / kg) to induce rat diabetes model, and rats in the normal group were intraperitoneally injected with the same dose of citric acid buffer. After 72 hours, blood was collected from the tail vein to detect the random blood glucose of rats in each group. Model success standard: random blood glucose ≥16.7mmol / L. After the rat diabetes model was successfully established, it was fed for 3 weeks, during which the blood glucose and body weight of rats in each group were tested on the 3rd, 7th, 14th and 21st days after STZ injection. After feeding for 3 weeks, the back skin of rats in each group was surgically cut with a diameter of 2cm and deep to the fascia to prepare the skin trauma model. On the day after surgery, rats in drug 1, drug 2, drug 3, drug 4, drug 5, and drug 6 groups were given corresponding drug ointments to apply to the skin wounds, with a thickness of no more than 0.5 cm, and covered with a layer of vaseline gauze for fixation, and the dressing was changed every 72 hours. Rats in the normal group and model group were given vaseline ointment in the same way. The rats were observed daily, and the skin wound area of ​​rats in each group was measured by taking pictures.

[0071] After STZ injection, the rats in the model group, drug group 1, drug group 2, drug group 3, drug group 4, drug group 5 and drug group 6 were observed to be thin, with reduced subcutaneous fat, dull fur, obvious hair loss, and obvious symptoms of polydipsia, polyphagia and polyuria. The normal group had white and smooth fur without the three more and one less symptoms.

[0072] By observing the changes in blood glucose of rats at different time points after STZ injection, it was found that there was no significant difference in the fasting blood glucose levels of rats in each group before STZ injection (P>0.05). On the 3rd, 7th, 14th and 21st days after STZ injection, the blood glucose of rats in the model group, drug group 1, drug group 2, drug group 3, drug group 4, drug group 5 and drug group 6 increased significantly, and the blood glucose level was higher than 16.7mmol / L. There was no significant change in the blood glucose of rats in the normal group (see Figure 1 ). The experimental results showed that the rats in the model group, drug group 1, drug group 2, drug group 3, drug group 4, drug group 5 and drug group 6 were all diabetic rats.

[0073] By observing the changes in the body weight of rats at different time points after STZ injection, it was found that there was no significant difference in the body weight of rats in each group before STZ injection (P>0.05). On the 3rd, 7th and 14th days after STZ injection, the body weight of rats in the model group, drug group 1, drug group 2, drug group 3, drug group 4, drug group 5 and drug group 6 increased slowly compared with the normal group (P<0.05). On the 21st day after STZ injection, the body weight of rats in the model group, drug group 1, drug group 2, drug group 3, drug group 4, drug group 5 and drug group 6 decreased compared with the body weight on the 14th day, while the body weight of rats in the normal group continued to increase; on the 21st day after STZ injection, the body weight of rats in the model group, drug group 1, drug group 2, drug group 3, drug group 4, drug group 5 and drug group 6 decreased significantly compared with the normal group (P<0.05). On days 3, 7, 14, and 21 after STZ injection, there was no significant difference in body weight among the model group, drug group 1, drug group 2, drug group 3, drug group 4, drug group 5, and drug group 6 (P>0.05) (see Figure 2 ). The results showed that after injection of STZ, the blood sugar level of rats increased and their body weight decreased, which was consistent with the clinical characteristics of diabetes, and the diabetic model of rats was successfully established.

[0074] By observing the skin wound healing of rats in each group, it was found that on the 0th day of skin wound preparation, there was no significant difference in the skin wound area of ​​rats in each group (P>0.05). On the 7th, 14th and 21st days after surgery, compared with the normal group, the skin wound area of ​​drug group 1, drug group 2, drug group 3, drug group 4, drug group 5 and drug group 6 increased significantly (P<0.05); on the 7th, 14th and 21st days after surgery, compared with the model group, the skin wound area of ​​drug group 1, drug group 2 and drug group 3 decreased, with no significant difference (P>0.05), and the skin wound area of ​​drug group 4, drug group 5 and drug group 6 decreased significantly (P<0.05), especially in drug group 5, but the wound area was still larger than that of the normal group (P<0.05) (see Figure 3 ).

[0075] The above results confirm that placenta, leeches and their mixtures all have certain effects in promoting the healing of diabetic skin, but the therapeutic effect of single-ingredient placenta or leeches on diabetic wounds is not significant. The mass ratio of placenta to leeches must reach a certain range to achieve significant effects on the treatment of diabetic wounds. Among them, the effect of promoting diabetic skin healing is best when the mass ratio of placenta to leeches is 10:1.

[0076] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit the present invention. Although the present invention has been described in detail with reference to the embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. A pharmaceutical composition for treating diabetic skin wounds, characterized in that: The pharmaceutical composition is composed of the following raw materials in parts by mass: 20-30 parts of roasted placenta particles and 2-3 parts of roasted leech particles.

2. The pharmaceutical composition according to claim 1, characterized in that The mass ratio of the roasted placenta particles to the roasted leech particles in the pharmaceutical composition is 10:

1.

3. The method for preparing the pharmaceutical composition according to any one of claims 1 or 2, characterized in that: The steps include: (1) roasting the placenta and grinding it to obtain placenta particles; (2) scalding leeches to death with boiling water, roasting them until they turn pale yellow, and grinding them to obtain leech granules; (3) The placenta particles obtained in step (1) and the leech particles obtained in step (2) are mixed in a mass ratio of (20-30):(2-3) to obtain a pharmaceutical composition.

4. The preparation method according to claim 3, characterized in that: The particle size of the placenta particles in step (1) is 70-80 μm.

5. The preparation method according to claim 3, characterized in that: The particle size of the leech particles in step (2) is 70-80 μm.

6. The preparation method according to claim 3, characterized in that: The leeches in step (2) are captured in May-June or September-October.

7. Use of the pharmaceutical composition according to any one of claims 1 or 2 in the preparation of a medicament for treating diabetic skin wounds.

8. The use according to claim 7, characterized in that: The dosage form of the medicine is an ointment preparation for external use.

9. An ointment for treating diabetic skin wounds, characterized in that: The ointment is prepared by mixing the pharmaceutical composition according to any one of claims 1 or 2 and vaseline in a mass ratio of 1:(5-10).

10. The method for preparing the ointment according to claim 9, characterized in that: The preparation method comprises adding the pharmaceutical composition of any one of claims 1 or 2 to sterilized vaseline for mixing, wherein the mass ratio of the pharmaceutical composition to the vaseline is 1:(5-10).

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