Efficient microbial fermentation method and application thereof in cosmetic raw material production

Abstract

The invention provides an efficient microbial fermentation method and application thereof in production of cosmetic raw materials, and the method comprises the following steps: (1) weighing radix astragali, crushing and sieving to obtain radix astragali powder, mixing 100 parts by mass of radix astragali powder with 600-800 parts by mass of purified water, and sterilizing to obtain a dispersion liquid; (2) adding 1 part by mass of lactobacillus plantarum and the dispersion liquid into a fermentation tank, uniformly mixing, and carrying out anaerobic fermentation to obtain fermentation liquor A; (3) adding 1 part by mass of lactobacillus bulgaricus into the fermentation liquor A, uniformly mixing, performing anaerobic fermentation, and performing sterilization treatment after fermentation to obtain fermentation liquor B; (4) adding 1 part by mass of bifidobacterium lactis into the fermentation liquor B, uniformly mixing, performing anaerobic fermentation, and performing sterilization treatment after the fermentation is completed to obtain fermentation liquor C; and (5) centrifuging the fermentation liquor C, discarding precipitate, collecting supernate, and performing spray drying on the supernate to obtain the astragalus extract. The fermentation method disclosed by the invention is high in yield of the astragalus extract.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and in particular relates to a high-efficiency microbial fermentation method and its application in the production of cosmetic raw materials. Background Art

[0002] Microbial fermentation technology is widely used in the field of cosmetics, mainly focusing on the following aspects: Production of active ingredients: Enrichment of natural ingredients: Microbial fermentation can enrich the active ingredients in plant resources, while reducing potential toxic side effects and improving the effectiveness and safety of cosmetic raw materials. Production of specific active ingredients: Microbial fermentation can produce active ingredients such as hyaluronic acid, ectoine, ergothioneine, etc., which have significant effects in moisturizing, anti-aging, and soothing the skin. Production of enzymes: Enzymes produced during the fermentation process, such as cellulase, protease, pectinase, etc., can be used for the pretreatment of cosmetic raw materials to improve the utilization rate of raw materials and the quality of products. Development of innovative raw materials: Proprietary strain fermentation products: Fermentation using special strains, such as SK-II's Pitera live cell yeast essence and Estee Lauder's bifida yeast fermentation product lysate, these fermentations are rich in active substances that are beneficial to the skin.

[0003] Plant fermentation: Through microbial fermentation, the concentration of active ingredients in plant extracts can be increased while reducing toxicity, such as the application of Centella asiatica fermentation liquid in anhydrous formula. Improve product characteristics: Improve absorbability: Fermentation products usually have a lower molecular weight and are more easily absorbed by the skin, thereby improving the absorbability and efficacy of the product. Increase safety: Fermentation technology can produce relatively safe products suitable for sensitive skin or special populations, such as pregnant women and children. Replace chemical synthesis: Replace active ingredients synthesized by chemical synthesis: Biofermentation technology can replace traditional chemical synthesis methods to produce ingredients such as peptides and oligopeptides, reduce costs and reduce environmental pollution. Innovative application of biotechnology: Microbial metabolic engineering: Through metabolic engineering to transform microorganisms, cosmetic raw materials with specific functions can be produced, such as ingredients with enhanced anti-inflammatory and antioxidant properties, while reducing energy consumption and pollution. The application of microbial fermentation technology in the preparation of cosmetic raw materials has become an important direction of research and development in the cosmetics industry. It not only improves the efficacy and safety of cosmetics, but also brings more innovative possibilities to the industry. With the continuous advancement of biotechnology, its application in the cosmetics field will be more extensive and in-depth in the future.

[0004] Astragalus is an herb widely used in traditional Chinese medicine and has attracted much attention for its various pharmacological activities. In recent years, astragalus and its extracts have also been increasingly used in the cosmetic field, mainly based on their skin care and beauty effects. The following are some of the main applications of astragalus and its extracts in cosmetics: Antioxidant and anti-aging: Astragalus contains a variety of antioxidant ingredients, such as flavonoids, polysaccharides, saponins, etc., which can scavenge free radicals, inhibit oxidative stress, and reduce signs of skin aging, such as wrinkles and sagging. Immunomodulatory and anti-inflammatory: Astragalus extract has immunomodulatory and anti-inflammatory effects, can reduce skin inflammatory reactions, and has a certain auxiliary effect in the treatment of skin problems such as acne and dermatitis. Promote skin repair and regeneration: The active ingredients in astragalus can promote the proliferation and differentiation of skin cells, accelerate wound healing, and help repair and rebuild the skin barrier. It is particularly suitable for the care of damaged or sensitive skin. Moisturizing and nourishing: Astragalus polysaccharides have good moisturizing properties, which can help the skin lock in moisture and maintain skin elasticity and luster. Whitening and spot lightening: Certain ingredients in astragalus extract can inhibit the activity of tyrosinase, reduce the production of melanin, help lighten spots and brighten the skin. Enhance skin defense: Astragalus extract can enhance the skin's defense mechanism, fight against external environmental stress, such as ultraviolet radiation, pollutants, etc., and protect the skin from damage. Astragalus extracts are used in cosmetics in various forms, including but not limited to creams, essences, masks, sunscreens, body lotions, etc. They are usually added to product formulas to enhance the efficacy of the product and meet consumers' pursuit of healthy and beautiful skin.

[0005] The application of astragaloside IV in cosmetics is mainly due to its multiple biologically active properties, such as antioxidant, anti-inflammatory, anti-aging and skin repair. The following are the specific effects and advantages of astragaloside IV in cosmetics: 1. Antioxidant effect. Astragaloside IV can effectively remove free radicals and reduce the damage of oxidative stress to the skin. Free radicals are one of the main factors leading to skin aging, dullness and cell damage. Through antioxidant effects, astragaloside IV can help maintain the health of the skin and prevent early aging. 2. Anti-inflammatory effect. Astragaloside IV has significant anti-inflammatory properties and can inhibit skin inflammatory reactions. This helps to reduce the symptoms of skin redness, sensitivity and discomfort caused by environmental pollution, ultraviolet radiation or other external stimuli, and improve the overall health of the skin. 3. Anti-aging effect. Astragaloside IV can regulate a variety of signaling pathways related to skin aging, promote collagen synthesis, enhance skin elasticity and firmness, and thus slow down the aging process of the skin. Its anti-aging effect makes it a common ingredient in anti-aging products such as creams and essences. 4. Promote skin repair. Astragaloside IV helps accelerate the repair and regeneration of skin cells, promote wound healing and the recovery of skin barrier function. This is especially important for the repair of damaged skin and the care of sensitive skin. 5. Moisturizing and nourishing. By enhancing the skin barrier function, astragaloside IV can improve the skin's hydration capacity, help the skin retain moisture, and prevent dryness and peeling. This makes it an ideal ingredient in moisturizing products. 6. Whitening effect. Some studies have shown that astragaloside IV may have the effect of inhibiting the production of melanin, which can help improve uneven skin tone and pigmentation problems, and enhance the skin's glossiness and whiteness.

[0006] Astragaloside IV is the main component of astragaloside saponins, which has anti-inflammatory, anti-fibrotic, antioxidant, immunomodulatory, metabolic regulation and other effects. However, in the prior art, whether using traditional alcohol extraction, ultrasonic extraction, supercritical extraction and other methods, the content of astragaloside IV in astragalus extract is low, which increases the production cost. Summary of the invention

[0007] The purpose of the present invention is to provide a high-efficiency microbial fermentation method and its application in the production of cosmetic raw materials.

[0008] In order to achieve the above object, the present invention provides the following technical solutions: A high-efficiency microbial fermentation method, characterized in that it comprises the following steps: (1) Weighing astragalus, crushing and sieving to obtain astragalus powder, mixing 100 parts by weight of the astragalus powder with 600-800 parts by weight of purified water, and sterilizing to obtain a dispersion; (2) adding 1 part by weight of Lactobacillus plantarum and the dispersion into a fermentation tank, mixing them evenly, adjusting the pH to 6.2-6.4, and performing anaerobically fermentation at 36-40° C. After the fermentation is completed, sterilizing treatment is performed to obtain fermentation liquid A; (3) adding 1 part by weight of Lactobacillus bulgaricus to fermentation liquid A and mixing evenly, adjusting the pH to 6.2-6.4, and performing anaerobically fermentation at 36-40° C. for 2-4 days. After the fermentation is completed, sterilizing treatment is performed to obtain fermentation liquid B; (4) adding 1 part by weight of Bifidobacterium lactis to the fermentation liquid B and mixing evenly, adjusting the pH to 6.2-6.4, performing anaerobically fermentation at 36-40° C., and sterilizing after the fermentation to obtain fermentation liquid C; (5) The fermentation liquid C is centrifuged, the precipitate is discarded, the supernatant is collected, and the supernatant is spray-dried to obtain an astragalus extract.

[0009] Furthermore, Lactobacillus plantarum has an activity of 10 billion CFU / g, a preservation number of CCTCC AB 2010210, and is preserved in the China Center for Type Culture Collection.

[0010] Furthermore, Bifidobacterium lactis, with a bacterial activity of 10 billion CFU / g, has a preservation number of SHBCCD24675 and is preserved in the Shanghai Collection Center of Microorganisms.

[0011] Furthermore, Lactobacillus bulgaricus, with an activity of 20 billion CFU / g, has a preservation number of CCTCCC B 20082295 and is preserved in the China Center for Type Culture Collection.

[0012] Lactobacillus plantarum: Classification: Lactobacillus plantarum belongs to the genus Lactobacillus and is a Gram-positive bacterium. Morphology and characteristics: It is a rod-shaped bacterium that can grow in aerobic and anaerobic environments (facultative anaerobic) and produces lactic acid during fermentation. Ecological environment: It is widely found in plants, dairy products and the human intestine. It is commonly found in fermented foods such as sauerkraut, kimchi, yogurt, etc.

[0013] Bifidobacterium lactis: Classification: Bifidobacterium lactis belongs to the genus Bifidobacterium and is a Gram-positive anaerobic bacterium. Morphology and characteristics: It is Y-shaped or branched, has the characteristics of acid and bile resistance, and can survive and reproduce in the intestines. Ecological environment: It mainly exists in the intestines of mammals and is one of the dominant flora in the intestines of infants and adults.

[0014] Lactobacillus delbrueckii subsp. bulgaricus: Classification: Lactobacillus delbrueckii subsp. bulgaricus is a lactic acid bacillus of the genus Lactobacillus. It is part of the subsp. delbrueckii. Morphology and characteristics: rod-shaped bacteria, a strict fermentative microorganism that mainly produces lactic acid through homotypic fermentation. Ecological environment: widely found in fermented dairy products, such as yogurt, especially in traditional Bulgarian yogurt.

[0015] The present invention uses Lactobacillus plantarum, Bifidobacterium lactis and Lactobacillus bulgaricus to ferment and extract Astragalus, so that the active substances in Astragalus can be fully extracted. Using Lactobacillus plantarum, Bifidobacterium lactis and Lactobacillus bulgaricus to ferment and extract Astragalus can increase the content of astragaloside IV in the Astragalus extract.

[0016] Furthermore, the sterilization method is: sterilization at 120-122°C for 12-17 minutes.

[0017] Furthermore, the astragalus is crushed and passed through a 50-80 mesh sieve.

[0018] Furthermore, the step (2) is carried out by anaerobic fermentation for 3-5 days.

[0019] Furthermore, the anaerobic fermentation in step (3) is carried out for 2-4 days.

[0020] Furthermore, the step (4) is anaerobic fermented for 1-2 days.

[0021] The present invention provides application of the above-mentioned high-efficiency microbial fermentation method in the production of cosmetic raw materials.

[0022] Compared with the prior art, the advantages and beneficial effects of the present invention are as follows: the present invention can fully extract the active substances in Astragalus by fermenting and extracting Astragalus with Lactobacillus plantarum, Bifidobacterium lactis and Lactobacillus bulgaricus, and can increase the content of Astragaloside IV in the Astragalus extract by fermenting and extracting Astragalus with Lactobacillus plantarum, Bifidobacterium lactis and Lactobacillus bulgaricus, which is mainly due to the following reasons: Enzymatic hydrolysis: the strains used in the present invention will produce a variety of enzymes during the fermentation process, such as cellulase, xylanase and α-glucosidase, etc. These enzymes can decompose polysaccharides and other complex compounds in Astragalus, thereby releasing more Astragaloside IV. Astragaloside IV is a saponin compound, and its precursor can be converted into Astragaloside IV through enzymatic hydrolysis to increase its content. Synergistic effect of metabolites: the strains used in the present invention will produce a variety of metabolites during the fermentation process, such as organic acids, short-chain fatty acids and antioxidants, etc. These metabolites can change the pH value and redox state of Astragalus, thereby affecting the stability and solubility of Astragaloside IV, making it easier to extract. Cell wall degradation: The fermentation of the strain used in the present invention can destroy the plant cell wall and increase the release of active ingredients in the cell. The cell wall of Astragalus is relatively thick, and conventional extraction methods may find it difficult to completely release the effective ingredients therein, while the degradation of the cell wall during the fermentation process can make astragaloside IV easier to extract. Biotransformation: The strain of the present invention has the ability of biotransformation and can convert the low-activity precursor substances in Astragalus into highly active astragaloside IV through metabolic pathways. This biotransformation can significantly increase the content of astragaloside IV in the extract. Improve solubility and bioavailability: The fermentation of the strain used in the present invention can improve the solubility and bioavailability of the Astragaloside extract.

[0023] In summary, fermentation extraction of Astragalus by Lactobacillus plantarum, Bifidobacterium lactis and Lactobacillus bulgaricus can increase the content of astragaloside IV in Astragalus extract through multiple mechanisms such as enzymatic hydrolysis, metabolite synergy, cell wall degradation and biotransformation. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] Example 1 This embodiment provides a high-efficiency microbial fermentation method, characterized in that it includes the following steps: (1) Weigh astragalus, crush it through a 50-mesh sieve to obtain astragalus powder, mix 100 parts by weight of the astragalus powder with 600 parts by weight of purified water, and sterilize to obtain a dispersion; (2) adding 1 part by weight of Lactobacillus plantarum and the dispersion into a fermentation tank, mixing them evenly, adjusting the pH to 6.2, and performing anaerobically fermentation at 36° C. for 3 days. After the fermentation is completed, sterilizing treatment is performed to obtain fermentation liquid A; (3) adding 1 part by weight of Lactobacillus bulgaricus to fermentation liquid A and mixing evenly, adjusting the pH to 6.2, and performing anaerobically fermentation at 36° C. for 2 days. After the fermentation is completed, sterilizing treatment is performed to obtain fermentation liquid B; (4) adding 1 part by weight of Bifidobacterium lactis to the fermentation liquid B and mixing evenly, adjusting the pH to 6.2, and performing anaerobically fermentation at 36° C. for 1 day. After the fermentation is completed, sterilizing treatment is performed to obtain fermentation liquid C; (5) The fermentation liquid C is centrifuged, the precipitate is discarded, the supernatant is collected, and the supernatant is spray-dried to obtain an astragalus extract.

[0026] Lactobacillus plantarum, active 10 billion CFU / g. Collection number: CCTCC AB 2010210. Deposited in China Center for Type Culture Collection.

[0027] Bifidobacterium lactis, with an activity of 10 billion CFU / g. Collection number: SHBCC D 24675. Deposited in Shanghai Microbiological Collection Center.

[0028] Lactobacillus bulgaricus, bacterial activity 20 billion CFU / g. Collection number: CCTCCC B 20082295; deposited in China Center for Type Culture Collection.

[0029] The sterilization method is: 121℃ sterilization for 15 minutes.

[0030] Example 2 This embodiment provides a high-efficiency microbial fermentation method, characterized in that it includes the following steps: (1) Weigh astragalus, crush and pass through an 80-mesh sieve to obtain astragalus powder, mix 100 parts by weight of the astragalus powder with 800 parts by weight of purified water, and sterilize to obtain a dispersion; (2) adding 1 part by weight of Lactobacillus plantarum and the dispersion into a fermentation tank, mixing them evenly, adjusting the pH to 6.4, and performing anaerobically fermentation at 40° C. for 5 days. After the fermentation is completed, sterilizing treatment is performed to obtain fermentation liquid A; (3) adding 1 part by weight of Lactobacillus bulgaricus to the fermentation liquid A and mixing evenly, adjusting the pH to 6.4, and performing anaerobically fermentation at 40° C. for 4 days. After the fermentation is completed, sterilizing treatment is performed to obtain fermentation liquid B; (4) adding 1 part by weight of Bifidobacterium lactis to the fermentation liquid B and mixing evenly, adjusting the pH to 6.4, and performing anaerobically fermentation at 40° C. for 2 days. After the fermentation is completed, sterilizing treatment is performed to obtain fermentation liquid C; (5) The fermentation liquid C is centrifuged, the precipitate is discarded, the supernatant is collected, and the supernatant is spray-dried to obtain an astragalus extract.

[0031] Lactobacillus plantarum, active 10 billion CFU / g. Collection number: CCTCC AB 2010210. Deposited in China Center for Type Culture Collection.

[0032] Bifidobacterium lactis, with an activity of 10 billion CFU / g. Collection number: SHBCC D 24675. Deposited in Shanghai Microbiological Collection Center.

[0033] Lactobacillus bulgaricus, bacterial activity 20 billion CFU / g. Collection number: CCTCCC B 20082295; deposited in China Center for Type Culture Collection.

[0034] The sterilization method is: sterilize at 121℃ for 15 minutes.

[0035] Example 3 A high-efficiency microbial fermentation method, characterized in that it comprises the following steps: (1) Weigh astragalus, crush and pass through a 50-mesh sieve to obtain astragalus powder, mix 100 parts by weight of the astragalus powder with 700 parts by weight of purified water, and sterilize to obtain a dispersion; (2) adding 1 part by weight of Lactobacillus plantarum and the dispersion into a fermentation tank, mixing them evenly, adjusting the pH to 6.3, and performing anaerobically fermentation at 37° C. for 4 days. After the fermentation is completed, sterilizing treatment is performed to obtain fermentation liquid A; (3) adding 1 part by weight of Lactobacillus bulgaricus to the fermentation liquid A and mixing evenly, adjusting the pH to 6.3, and performing anaerobically fermentation at 37° C. for 3 days. After the fermentation is completed, sterilizing treatment is performed to obtain fermentation liquid B; (4) adding 1 part by weight of Bifidobacterium lactis to the fermentation liquid B and mixing evenly, adjusting the pH to 6.3, and performing anaerobically fermentation at 37° C. for 1.5 days. After the fermentation is completed, sterilizing treatment is performed to obtain fermentation liquid C; (5) The fermentation liquid C is centrifuged, the precipitate is discarded, the supernatant is collected, and the supernatant is spray-dried to obtain an astragalus extract.

[0036] Lactobacillus plantarum, active 10 billion CFU / g. Collection number: CCTCC AB 2010210. Deposited in China Center for Type Culture Collection.

[0037] Bifidobacterium lactis, with an activity of 10 billion CFU / g. Collection number: SHBCC D 24675. Deposited in Shanghai Microbiological Collection Center.

[0038] Lactobacillus bulgaricus, bacterial activity 20 billion CFU / g. Collection number: CCTCCC B 20082295; deposited in China Center for Type Culture Collection.

[0039] The sterilization method is: 121℃ sterilization for 15 minutes.

[0040] Comparative Example 1 The difference between this comparative example and Example 1 is that Lactobacillus bulgaricus is replaced by Lactobacillus brevis. Lactobacillus brevis, bacterial activity 10 billion CFU / g. Deposit number: SHBCCD14346. Deposited in Shanghai Microbiological Collection Center.

[0041] A high-efficiency microbial fermentation method, characterized in that it comprises the following steps: (1) Weigh astragalus, crush it through a 50-mesh sieve to obtain astragalus powder, mix 100 parts by weight of the astragalus powder with 600 parts by weight of purified water, and sterilize to obtain a dispersion; (2) adding 1 part by weight of Lactobacillus plantarum and the dispersion into a fermentation tank, mixing them evenly, adjusting the pH to 6.2, and performing anaerobically fermentation at 36° C. for 3 days. After the fermentation is completed, sterilizing treatment is performed to obtain fermentation liquid A; (3) adding 1 part by weight of Lactobacillus brevis to the fermentation liquid A and mixing evenly, adjusting the pH to 6.2, and performing anaerobically fermentation at 36° C. for 2 days. After the fermentation is completed, sterilizing treatment is performed to obtain fermentation liquid B; (4) adding 1 part by weight of Bifidobacterium lactis to the fermentation liquid B and mixing evenly, adjusting the pH to 6.2, and performing anaerobically fermentation at 36° C. for 1 day. After the fermentation is completed, sterilizing treatment is performed to obtain fermentation liquid C; (5) The fermentation liquid C is centrifuged, the precipitate is discarded, the supernatant is collected, and the supernatant is spray-dried to obtain an astragalus extract.

[0042] Lactobacillus plantarum, active 10 billion CFU / g. Collection number: CCTCC AB 2010210. Deposited in China Center for Type Culture Collection.

[0043] Bifidobacterium lactis, with an activity of 10 billion CFU / g. Collection number: SHBCC D 24675. Deposited in Shanghai Microbiological Collection Center.

[0044] The sterilization method is: 121℃ sterilization for 15 minutes.

[0045] Comparative Example 2 The difference between this comparative example and Example 1 is that Bifidobacterium lactis is replaced by Lactobacillus acidophilus. The bacterial activity of Lactobacillus acidophilus is 20 billion CFU / g. The deposit number is CCTCCAB2010208. It is deposited in China Center for Type Culture Collection.

[0046] A high-efficiency microbial fermentation method, characterized in that it comprises the following steps: (1) Weigh astragalus, crush it through a 50-mesh sieve to obtain astragalus powder, mix 100 parts by weight of the astragalus powder with 600 parts by weight of purified water, and sterilize to obtain a dispersion; (2) adding 1 part by weight of Lactobacillus plantarum and the dispersion into a fermentation tank, mixing them evenly, adjusting the pH to 6.2, and performing anaerobically fermentation at 36° C. for 3 days. After the fermentation is completed, sterilizing treatment is performed to obtain fermentation liquid A; (3) adding 1 part by weight of Lactobacillus bulgaricus to fermentation liquid A and mixing evenly, adjusting the pH to 6.2, and performing anaerobically fermentation at 36° C. for 2 days. After the fermentation is completed, sterilizing treatment is performed to obtain fermentation liquid B; (4) adding 1 part by weight of Lactobacillus acidophilus to the fermentation liquid B and mixing evenly, adjusting the pH to 6.2, and performing anaerobically fermentation at 36° C. for 1 day. After the fermentation is completed, sterilizing treatment is performed to obtain fermentation liquid C; (5) The fermentation liquid C is centrifuged, the precipitate is discarded, the supernatant is collected, and the supernatant is spray-dried to obtain an astragalus extract.

[0047] Lactobacillus plantarum, active 10 billion CFU / g. Collection number: CCTCC AB 2010210. Deposited in China Center for Type Culture Collection.

[0048] Lactobacillus bulgaricus, bacterial activity 20 billion CFU / g. Collection number: CCTCCC B 20082295; deposited in China Center for Type Culture Collection.

[0049] The sterilization method is: 121℃ sterilization for 15 minutes.

[0050] Comparative Example 3 The difference between this comparative example and Example 1 is that Lactobacillus plantarum is replaced by Lactobacillus helveticus. Lactobacillus helveticus, bacterial activity 20 billion CFU / g. Deposit number: CCTCCAB2010205; deposited in China Center for Type Culture Collection.

[0051] A high-efficiency microbial fermentation method, characterized in that it comprises the following steps: (1) Weigh astragalus, crush it through a 50-mesh sieve to obtain astragalus powder, mix 100 parts by weight of the astragalus powder with 600 parts by weight of purified water, and sterilize to obtain a dispersion; (2) adding 1 part by weight of Lactobacillus helveticus and the dispersion into a fermentation tank, mixing them evenly, adjusting the pH to 6.2, and performing anaerobically fermentation at 36° C. for 3 days. After the fermentation is completed, sterilizing treatment is performed to obtain fermentation liquid A; (3) adding 1 part by weight of Lactobacillus bulgaricus to fermentation liquid A and mixing evenly, adjusting the pH to 6.2, and performing anaerobically fermentation at 36° C. for 2 days. After the fermentation is completed, sterilizing treatment is performed to obtain fermentation liquid B; (4) adding 1 part by weight of Bifidobacterium lactis to the fermentation liquid B and mixing evenly, adjusting the pH to 6.2, and performing anaerobically fermentation at 36° C. for 1 day. After the fermentation is completed, sterilizing treatment is performed to obtain fermentation liquid C; (5) The fermentation liquid C is centrifuged, the precipitate is discarded, the supernatant is collected, and the supernatant is spray-dried to obtain an astragalus extract.

[0052] Bifidobacterium lactis, with an activity of 10 billion CFU / g. Collection number: SHBCC D 24675. Deposited in Shanghai Microbiological Collection Center.

[0053] Lactobacillus bulgaricus, bacterial activity 20 billion CFU / g. Collection number: CCTCCC B 20082295; deposited in China Center for Type Culture Collection.

[0054] The sterilization method is: 121℃ sterilization for 15 minutes.

[0055] Comparative Example 4 This comparative example provides a method for preparing an astragalus extract, comprising the following steps: (1) Grind the astragalus and pass it through a 60-mesh sieve to obtain fine powder; (2) Mix the micro powder with a mass ratio of 1:9 and an ethanol aqueous solution with a volume fraction of 70%, heat and reflux for extraction for 3 times, each extraction for 60 minutes, filter, and combine the filtrate to obtain an extract; (3) Centrifuge the extract, take the supernatant, concentrate and dry it to obtain the astragalus extract.

[0056] Performance Testing The yield of the astragalus extracts of Examples 1-3 and Comparative Examples 1-4 was determined as follows: the mass of the astragalus extract / the mass of the astragalus 100%. Detect the content of astragaloside IV in 1g of Astragalus extract.

[0057] Table 1 Performance test results

[0058] From the results, it can be seen that the astragaloside IV conversion rate of Examples 1-4 of the present invention is the highest, which indicates that the strain has a significant effect on the astragaloside IV conversion rate.

[0059] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A high-efficiency microbial fermentation method, characterized in that: The following steps are involved: (1) Weighing astragalus, crushing and sieving to obtain astragalus powder, mixing 100 parts by weight of the astragalus powder with 600-800 parts by weight of purified water, and sterilizing to obtain a dispersion; (2) adding 1 part by weight of Lactobacillus plantarum and the dispersion into a fermentation tank, mixing them evenly, adjusting the pH to 6.2-6.4, and performing anaerobically fermentation at 36-40° C. After the fermentation is completed, sterilizing treatment is performed to obtain fermentation liquid A; (3) adding 1 part by weight of Lactobacillus bulgaricus to fermentation liquid A and mixing evenly, adjusting the pH to 6.2-6.4, performing anaerobically fermentation at 36-40° C., and sterilizing after the fermentation to obtain fermentation liquid B; (4) adding 1 part by weight of Bifidobacterium lactis to the fermentation liquid B and mixing evenly, adjusting the pH to 6.2-6.4, performing anaerobically fermentation at 36-40° C., and sterilizing after the fermentation to obtain fermentation liquid C; (5) The fermentation liquid C is centrifuged, the precipitate is discarded, the supernatant is collected, and the supernatant is spray-dried to obtain an astragalus extract.

2. The high-efficiency microbial fermentation method according to claim 1, characterized in that: Lactobacillus plantarum, bacterial activity 10 billion CFU / g, preservation number: CCTCC AB 2010210, preserved in China Center for Type Culture Collection.

3. The high-efficiency microbial fermentation method according to claim 1, characterized in that: Bifidobacterium lactis, bacterial activity 10 billion CFU / g, preservation number: SHBCC D 24675, preserved in Shanghai Microbiological Collection Center.

4. The high-efficiency microbial fermentation method according to claim 1, characterized in that: Lactobacillus bulgaricus, bacterial activity 20 billion CFU / g, preservation number: CCTCCC B 20082295, preserved in China Center for Type Culture Collection.

5. The high-efficiency microbial fermentation method according to claim 1, characterized in that: The sterilization method is: 120-122℃ sterilization for 12-17min.

6. The high-efficiency microbial fermentation method according to claim 1, characterized in that: Astragalus root was crushed and passed through a 50-80 mesh sieve.

7. The high-efficiency microbial fermentation method according to claim 1, characterized in that: The step (2) is anaerobic fermentation for 3-5 days.

8. The high-efficiency microbial fermentation method according to claim 1, characterized in that: The step (3) is anaerobic fermented for 2-4 days.

9. The high-efficiency microbial fermentation method according to claim 1, characterized in that: The step (4) is anaerobic fermentation for 1-2 days.

10. Use of the high-efficiency microbial fermentation method according to any one of claims 1 to 9 in the production of cosmetic raw materials.

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