Full-automatic multifunctional pilot platform system and chromatographic system thereof

Abstract

The invention provides a full-automatic multifunctional pilot platform system and a chromatographic system thereof. The platform system comprises a first input main pipeline with a first input pump and a second input main pipeline with a second input pump, the first input main pipeline is connected with a plurality of input pipelines, and on-off of the first input main pipeline is controlled through corresponding valves; the second input main pipeline is also respectively connected with the plurality of input pipelines, and the on-off of the second input main pipeline is controlled through corresponding valves; the outlet ends of the input pipelines are connected with liquid inlet ports of the corresponding movable installation modules respectively, and the movable installation modules are further provided with a plurality of backflow ports and a plurality of liquid outlet ports. The liquid outlet port is respectively connected with a plurality of collecting pipelines through output pipelines; according to the chromatographic system, an RTF column is mounted in a movable mounting module; during use, two tangential flow chromatographic columns are adopted and operate alternately: one is in a loading mode, and the other is in an elution mode; the device has the advantages that modularization is achieved, multiple functions are achieved according to different process requirements, and the downstream production process of biological medicine is met.

Description

Technical Field

[0001] The present invention relates to a biomedical downstream platform, and in particular to a fully automatic multifunctional pilot platform system and a chromatography system thereof. Background Art

[0002] Downstream processes in biopharmaceuticals include filtration, which is mainly used to remove impurities such as cell debris, bacteria, viruses, etc. Filtration includes NFF and TFF; purification, which is mainly used to separate, purify and refine target products, involving the use of chromatography columns.

[0003] In the prior art, for example, the patent number is: 202120067489.X, and the utility model name is a hydraulically driven dynamic chromatography device. When the separation liquid needs to be added, the liquid is injected from the liquid inlet and flows out from the liquid outlet for collection; or, for example, the patent number is: 201820028656.8, and the utility model name is a moving beam type fully automatic chromatography column, the structure of which is a sieve plate, a diverter plate and a base at the bottom, and a lower nozzle is arranged at the center of the sieve plate. When the separation liquid needs to be added, the liquid enters from the chromatography port of the upper nozzle, flows out from the chromatography port of the lower nozzle for collection, or enters from the chromatography port of the lower nozzle, flows out from the chromatography port of the upper nozzle for collection. Its disadvantages are: 1. It cannot achieve flushing or elution of the upper column head; 2. It does not support reverse flushing of the filter plate (sieve plate), which is easy to cause blockage; 3. It cannot achieve high linear flow rate and overload loading at the same time, and the dynamic loading capacity is low. The applicant has proposed a tangential flow chromatography column, with patent number: 202220697921.8, and the patent name is a radial and tangential flow bottom for a chromatography column and a chromatography system used in combination. The tangential flow structure is adopted to achieve tangential flow filtration and simultaneous chromatographic loading, thereby achieving high linear flow rate and overload loading, and achieving extremely high dynamic loading capacity.

[0004] Therefore, the downstream technology of biomedicine needs to build different platforms for different processes to achieve the corresponding processes, so different system combinations are required. The disadvantages are: there are many types of platforms, which have limitations. One set of platforms can only meet the corresponding processes and cannot achieve platform sharing. Therefore, the present invention can modularize the platform to achieve different processes and reduce costs. Summary of the invention

[0005] In order to overcome the deficiencies of the above-mentioned prior art, the present invention provides a fully automatic multifunctional pilot platform system and a chromatography system thereof, which can be modularized and multifunctional according to different process requirements, meet the downstream production process of biomedicine, and achieve the effect of reducing costs and increasing efficiency.

[0006] In order to achieve the above object, the technical solution adopted by the present invention is:

[0007] In response to the above technical problems, the first aspect of the present invention discloses a fully automatic multifunctional pilot platform system, which includes a first input main pipeline with a first input pump and a second input main pipeline with a second input pump; the first input main pipeline is connected to multiple input pipelines and is controlled on and off by corresponding valves; the second input main pipeline is also respectively connected to the above-mentioned multiple input pipelines and is controlled on and off by corresponding valves; the outlet ends of the multiple input pipelines are respectively connected to the liquid inlet ports of their respective corresponding movable installation modules, and the movable installation modules are also provided with multiple reflux ports and multiple liquid outlet ports; the liquid outlet ports are respectively connected to multiple collection pipelines through output pipelines.

[0008] In some embodiments, the movable installation module can be used to install a chromatography column.

[0009] In some embodiments, there are two input pipes; there are two corresponding liquid inlet ports; there are two reflux ports; there are two liquid outlet ports; and there are two output pipes.

[0010] In some embodiments, the first input main pipeline and the second input main pipeline are connected to the two input pipelines through a first valve array group; the first valve array group includes four valves connected end to end to form a rectangular valve array; one group of opposite sides of the rectangular valve array are respectively connected to the corresponding first input main pipeline and the second input main pipeline; another group of opposite sides of the rectangular valve array are respectively connected to the corresponding two input pipelines.

[0011] In some embodiments, two collecting pipes are provided.

[0012] In some embodiments, the two output pipes are connected to the two collecting pipes through a second valve array group; the second valve array group includes four valves connected end to end to form a rectangular valve array; one group of opposite sides of the rectangular valve array are respectively connected to the corresponding output pipes; and another group of opposite sides of the rectangular valve array are respectively connected to the corresponding two collecting pipes.

[0013] In some embodiments, the second input main pipeline is connected to two collecting pipelines respectively through two valves.

[0014] In some embodiments, one side of the output end of the second input pump of the second input main pipeline is connected to the first input main pipeline through a valve.

[0015] The second aspect disclosed in the present invention proposes a fully automatic multifunctional chromatography system, which includes multiple RTF chromatography columns, the inlet end at the bottom of each RTF chromatography column is connected to the corresponding liquid inlet port, and the reflux end at the bottom of each RTF chromatography column is connected to the corresponding reflux port; the top outlet end of each RTF chromatography column is connected to the liquid outlet port; each of the liquid inlet ports is connected to the outlet end of the corresponding input pipeline, and the inlet end of each input pipeline is respectively connected to a first input main pipeline with a first input pump and a second input main pipeline with a second input pump, and the on-off is controlled by a corresponding valve; each of the liquid outlet ports is respectively connected to the inlet end of the corresponding output pipeline, and the outlet end of each output pipeline is connected to multiple collection pipelines.

[0016] In some embodiments, an air trap is provided on the first input main pipe.

[0017] In some embodiments, a mixer is provided on the first input main pipeline.

[0018] In some embodiments, the RTF chromatography columns are provided with two.

[0019] In some embodiments, the first input main pipeline and the second input main pipeline are connected to the two input pipelines through a first valve array group; the first valve array group includes four valves connected end to end to form a rectangular valve array; one group of opposite sides of the rectangular valve array are respectively connected to the corresponding first input main pipeline and the second input main pipeline; another group of opposite sides of the rectangular valve array are respectively connected to the corresponding two input pipelines.

[0020] In some embodiments, the input pipeline and the output pipeline in each of the RTF chromatography columns are connected via a valve.

[0021] In some embodiments, two collecting pipes are provided.

[0022] In some embodiments, the two output pipes are connected to the two collecting pipes through a second valve array group; the second valve array group includes four valves connected end to end to form a rectangular valve array; one group of opposite sides of the rectangular valve array are respectively connected to the corresponding output pipes; and another group of opposite sides of the rectangular valve array are respectively connected to the corresponding two collecting pipes.

[0023] In some embodiments, the second input main pipeline is connected to two collecting pipelines respectively through two valves.

[0024] In some embodiments, the inlet end of the second input main pipeline is connected to the first input main pipeline through a valve.

[0025] In some embodiments, the two reflux ports are respectively connected to corresponding reflux pipes, and the reflux pipes are respectively connected to filter modules.

[0026] In some embodiments, the two return pipes are connected to the filter module through a third valve array group, and the third valve array group includes four valves connected end to end to form a rectangular valve array; one group of opposite sides of the rectangular valve array are respectively connected to the corresponding return pipes; one side of the other group of opposite sides of the rectangular valve array is connected to the filter module, and the other side is connected to the waste pipe.

[0027] In some embodiments, the filtration module includes a filtration input main pipeline, on which a third input pump is provided, one end of the filtration input main pipeline is connected to the third valve array group, and the other end of the filtration input main pipeline is connected to the fourth valve array group, the fourth valve array includes four valves connected end to end to form a rectangular valve array, the filtration input main pipeline is connected to one side of a group of opposite sides of the rectangular valve array, and the other side is connected to a waste discharge pipeline; the other group of opposite sides of the matrix are respectively connected to one end of the corresponding filter; the other ends of the two filters are respectively connected to the fifth valve array group; the fifth valve array group includes four valves connected end to end to form a rectangular valve array; the other ends of the two filters are respectively connected to a group of opposite sides of the rectangular valve array; one side of the other group of opposite sides of the rectangular valve array is connected to the liquid input pipeline, and the other side of the other group of opposite sides of the rectangular valve array is connected to the first input main pipeline.

[0028] In some embodiments, one side of a group of opposite sides of the fifth valve array group is connected to the liquid input pipeline, and the other side of a group of opposite sides of the rectangular valve array group is connected to the first input main pipeline and the waste discharge pipeline.

[0029] Beneficial effects of the present invention:

[0030] 1. The present invention adopts a movable installation module to expand the platform, realize modularization, and realize multi-function according to different process requirements to meet the downstream production process of biomedicine.

[0031] 2. Two tangential flow chromatography columns are used in the present invention, which are operated alternately: one is in loading mode and the other is in elution mode. By doing so, the feed can be continuously loaded into the RTF column with almost no residue in the flow path and circulation loop, and the circulation loop does not have to be stopped and restarted many times.

[0032] 3. A circulation loop is used at the reflux end of the tangential flow to drive the circulation while loading the tangential flow chromatography column.

[0033] Instruction Manual

[0034] Figure 1 It is a structural schematic diagram of the present invention.

[0035] Figure 2 It is a structural diagram of the fully automatic multifunctional chromatography system of the present invention. DETAILED DESCRIPTION

[0036] The present invention will be further described below in conjunction with the accompanying drawings.

[0037] The technical content of the present invention is described below by specific embodiments. Those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments. Those skilled in the art can make various modifications and changes without departing from the spirit of the present invention.

[0038] Before describing the specific embodiments of the present disclosure in detail, some terms used in the present disclosure are first explained.

[0039] Unless otherwise defined below, the meaning of all technical terms and scientific terms used herein is intended to be the same as those commonly understood by those skilled in the art. Mentioning the technology used in this article is intended to refer to the technology commonly understood in the art, including those changes in technology that are obvious to those skilled in the art or the replacement of equivalent technology. Although it is believed that the following terms are well understood by those skilled in the art, the following definitions are still set forth to better explain the present invention. When a trade name appears in this article, it is intended to refer to the corresponding commodity. All patents, published patent applications and publications cited herein are incorporated herein by reference.

[0040] Unless otherwise indicated herein, multiple references such as "a", "the", include plural references. The expression "one or more" or "at least one" may mean 1, 2, 3, 4, 5, 6, 7, 8, 9 or more.

[0041] As used herein, the terms “connected”, “connected”, “coupled” or “coupled” and the like are not limited to direct connections but also include indirect connections.

[0042] As used herein, a "sample" is a biomolecule, including proteins, nucleic acids, lipids, carbohydrates, small nucleotides, amino acids and their derivatives.

[0043] As used herein, the term "online monitoring" or "real-time monitoring" refers to real-time detection of certain parameters or properties of buffers, reaction fluids, and fluids flowing out of flow reactors during use of the chromatography system, such as pH, pressure, flow rate, conductivity, etc. Unlike offline detection or analysis, online monitoring or real-time monitoring can provide real-time feedback of the detection results.

[0044] For example, the A2 Buffer storage tank, A3 Buffer storage tank, A4 Buffer storage tank, A5Buffer storage tank, P1 storage tank, P1-W storage tank, P2 storage tank, P2-W storage tank, hot WFI storage tank, and cold WFI storage tank mentioned in this article refer to tanks used to store different solutions, not tanks with specific limitations, and any container that can realize the storage function is sufficient.

[0045] The position relationship words "up", "down", "left", "right", "front", "back", etc. involved in this article are determined according to the layout direction of the drawings in the specification. They are only used to indicate relative position relationships. When the absolute position of the object being described changes, the relative position relationship may also change accordingly.

[0046] The RTF chromatography column mentioned in this article is a patented chromatography column independently developed by our company, with patent number: 202220697921.8. It has radial and tangential flow structures, and can load samples through the tangential flow at the bottom of the column, while the upper part of the column head can be flushed and eluted at the same time, with cyclic loading and a balance liquid cleaning mode, thereby achieving high linear flow rate and overload loading, and achieving extremely high dynamic loading capacity.

[0047] The application scenarios of this patent are laboratory scale and pilot scale, and the maximum flow range of a single pump is 1000ml / min.

[0048] like Figure 1As shown, the multifunctional fully automatic pilot platform system includes a first input main pipeline 1, the inlet end of the first input main pipeline 1 is respectively connected to the outlets of multiple liquid storage tanks including an A2 Buffer liquid storage tank and a sample liquid storage tank; the outlets of the two liquid storage tanks are respectively provided with a first valve 106 and a second valve 104; the first input main pipeline 1 is respectively provided with a first input pump 2, a first pressure sensor 3, a first flow meter 4, a third valve 5, a mixer 6, and an air trap 7 from left to right; the inlet end of the air trap 7 is connected to the first input main pipeline 1 through a fourth valve 8, and the outlet end of the air trap 7 is connected to the first input main pipeline 1 through a fifth valve 9, and a sixth valve 10 is provided on the pipelines at the inlet and outlet ends of the air trap 7. The second input main pipeline 11, the inlet end of the second input main pipeline 11 is respectively connected to the outlets of multiple liquid storage tanks, including the A3 Buffer liquid storage tank, the A4 Buffer liquid storage tank, and the A5 Buffer liquid storage tank; the outlets of the three liquid storage tanks are respectively provided with a seventh valve 102, an eighth valve 101, and a ninth valve 103; the outlet of the A3 Buffer liquid storage tank is connected to the inlet end of the first input main pipeline 1 through a tenth valve 105. The second input main pipeline 11 is respectively provided with a second input pump 12, a second pressure sensor 13, a second flowmeter 14, an eleventh valve 15, and a twelfth valve 16 from left to right; the first input main pipeline 1 and the second input main pipeline 11 are connected through a thirteenth valve 17, one end of the thirteenth valve 17 is connected between the third valve 5 and the mixer 6, and the other end is connected between the second flowmeter 14 and the eleventh valve 15.

[0049] The first valve array group 17, the first valve array group 17 is a rectangular valve array formed by connecting four valves at the end, as shown in the figure, the fourteenth valve 18, the fifteenth valve 19, the sixteenth valve 20, and the seventeenth valve 21 in the clockwise direction. The outlet end of the first input main pipeline 1 is connected between the fourteenth valve 18 and the seventeenth valve 21, and the outlet end of the twelfth valve 16 of the second input main pipeline 11 is connected between the fifteenth valve 19 and the sixteenth valve 20. One end of the first input pipeline 22 is connected between the fourteenth valve 18 and the fifteenth valve 19, and the other end of the first input pipeline 22 is connected to the first liquid inlet port 24 of the movable installation module 23; the first input pipeline 22 is provided with an eighteenth valve 25; the sixteenth valve 20 and the seventeenth valve 21 are connected to one end of the second input pipeline 26, and the other end of the second input pipeline 26 is connected to the second liquid inlet port 27 of the movable installation module 23, and the second input pipeline 26 is provided with a nineteenth valve 29.

[0050] The movable installation module 23 further includes a first reflux port 30, a second reflux port 31, a first liquid outlet port 32 and a second liquid outlet port 33. The first liquid outlet port 32 is connected to one end of a first output pipe 34, and the second liquid outlet port 33 is connected to one end of a second output pipe 35; the other ends of the first output pipe 34 and the second output pipe 35 are respectively connected to a second valve array group 36, and the second valve array group 36 is a rectangular valve array formed by four valves connected at the end, as shown in the figure, the 20th valve 37, the 21st valve 38, the 22nd valve 39 and the 23rd valve 40 in the clockwise direction; the other end of the first output pipe 34 is connected between the 21st valve 38 and the 22nd valve 39, and the other end of the second output pipe 35 is connected between the 20th valve 37 and the 23rd valve 40. The inlet end of the first collecting pipeline 41 is connected between the 20th valve 37 and the 21st valve 38; the inlet end of the second collecting pipeline 42 is connected between the 22nd valve 39 and the 23rd valve 40; the first collecting pipeline 41 is connected to the P1 liquid storage tank and the P1-W liquid storage tank through the 24th valve 43 and the 25th valve 44 respectively. The second collecting pipeline 42 is connected to the P2 liquid storage tank and the P2-W liquid storage tank through the 26th valve 45 and the 27th valve 46 respectively. The outlet of the twelfth valve 16 of the second input main pipeline 11 is connected to the first collecting pipeline 41 and the second collecting pipeline 42 through the first intermediate pipeline connection 47 and the second intermediate pipeline 48 respectively. The two ends of the first intermediate pipeline 48 are respectively provided with the 28th valve 49 and the 29th valve 50; the two ends of the second intermediate pipeline 48 are respectively provided with the 30th valve 51 and the 31st valve 52.

[0051] A thirty-second valve 53 is disposed on the first output pipeline 34. A thirty-third valve 54 is disposed on the second output pipeline 35.

[0052] The first reflux port 30 of the movable installation module 23 is connected to the inlet end of the filter module 56 through the thirty-fourth valve 55; the second reflux port 31 of the movable installation module 23 is connected to the inlet end of the filter module 56 through the thirty-fifth valve 57; the outlet end of the filter module 56 is connected between the third valve 5 of the first input main pipeline 1 and the mixer 6 through the thirty-sixth valve 58.

[0053] Reference Figure 2 A fully automatic multifunctional chromatography system is shown, in which two RTF chromatography columns are installed on the above-mentioned pilot platform system.

[0054] It includes a first input main pipeline 1, the inlet end of the first input main pipeline 1 is respectively connected to the outlets of multiple liquid storage tanks including an A2 Buffer liquid storage tank and a sample liquid storage tank; the outlets of the two liquid storage tanks are respectively provided with a first valve 106 and a second valve 104; the first input main pipeline 1 is respectively provided with a first input pump 2, a first pressure sensor 3, a first flow meter 4, a third valve 5, a mixer 6, and an air trap 7 from left to right; the inlet end of the air trap 7 is connected to the first input main pipeline 1 through a fourth valve 8, the outlet end of the air trap 7 is connected to the first input main pipeline 1 through a fifth valve 9, and a sixth valve 10 is provided on the pipelines at the inlet and outlet ends of the air trap 7. The second input main pipeline 11, the inlet end of the second input main pipeline 11 is respectively connected to the outlets of multiple liquid storage tanks, including the A3 Buffer liquid storage tank, the A4 Buffer liquid storage tank, and the A5 Buffer liquid storage tank; the outlets of the three liquid storage tanks are respectively provided with a seventh valve 102, an eighth valve 101, and a ninth valve 103; the outlet of the A3 Buffer liquid storage tank is connected to the inlet end of the first input main pipeline 1 through a tenth valve 105. The second input main pipeline 11 is respectively provided with a second input pump 12, a second pressure sensor 13, a second flowmeter 14, an eleventh valve 15, and a twelfth valve 16 from left to right; the first input main pipeline 1 and the second input main pipeline 11 are connected through a thirteenth valve 17, one end of the thirteenth valve 17 is connected between the third valve 5 and the mixer 6, and the other end is connected between the second flowmeter 14 and the eleventh valve 15.

[0055] The first valve array group 17, the first valve array group 17 is a rectangular valve array formed by connecting four valves at the end, as shown in the figure, the fourteenth valve 18, the fifteenth valve 19, the sixteenth valve 20, and the seventeenth valve 21 in the clockwise direction. The outlet end of the first input main pipeline 1 is connected between the fourteenth valve 18 and the seventeenth valve 21, and the outlet end of the twelfth valve 16 of the second input main pipeline 11 is connected between the fifteenth valve 19 and the sixteenth valve 20. One end of the first input pipeline 22 is connected between the fourteenth valve 18 and the fifteenth valve 19, and the other end of the first input pipeline 22 is connected to the bottom inlet of the first RTF chromatography column 65 through the first liquid inlet port 24 of the movable installation module 23; the first input pipeline 22 is provided with an eighteenth valve 25; the sixteenth valve 20 and the seventeenth valve 21 are connected to one end of the second input pipeline 26, and the other end of the second input pipeline 26 is connected to the inlet end of the second RTF chromatography column 66 through the second liquid inlet port 27 of the movable installation module 23, and the second input pipeline 26 is provided with a nineteenth valve 29.

[0056] The movable installation module 23 further includes a first reflux port 30, a second reflux port 31, a first liquid outlet port 32 and a second liquid outlet port 33. The first RTF chromatography column 65 is connected to one end of the first output pipe 34 through the first liquid outlet port 32, and the second RTF chromatography column 66 is connected to one end of the second output pipe 35 through the second liquid outlet port 33; the other ends of the first output pipe 34 and the second output pipe 35 are respectively connected to the second valve array group 36, and the second valve array group 36 is a rectangular valve array formed by four valves connected at the end, as shown in the figure, the 20th valve 37, the 21st valve 38, the 22nd valve 39 and the 23rd valve 40 in the clockwise direction; the other end of the first output pipe 34 is connected between the 21st valve 38 and the 22nd valve 39, and the other end of the second output pipe 35 is connected between the 20th valve 37 and the 23rd valve 40. The inlet end of the first collecting pipeline 41 is connected between the 20th valve 37 and the 21st valve 38; the inlet end of the second collecting pipeline 42 is connected between the 22nd valve 39 and the 23rd valve 40; the first collecting pipeline 41 is connected to the P1 liquid storage tank and the P1-W liquid storage tank through the 24th valve 43 and the 25th valve 44 respectively. The second collecting pipeline 42 is connected to the P2 liquid storage tank and the P2-W liquid storage tank through the 26th valve 45 and the 27th valve 46 respectively. The outlet of the twelfth valve 16 of the second input main pipeline 11 is connected to the first collecting pipeline 41 and the second collecting pipeline 42 through the first intermediate pipeline connection 47 and the second intermediate pipeline 48 respectively. The two ends of the first intermediate pipeline 48 are respectively provided with the 28th valve 49 and the 29th valve 50; the two ends of the second intermediate pipeline 48 are respectively provided with the 30th valve 51 and the 31st valve 52.

[0057] The first output pipeline 34 is provided with a 32nd valve 53 and a 37th valve 59. The second output pipeline 35 is provided with a 33rd valve 54 and a 38th valve 60; a first connecting pipeline 61 is provided between the first input pipeline 22 and the first output pipeline 34; one end of the first connecting pipeline 61 is connected between the 18th valve 25 and the first valve array group 17, and the other end of the first connecting pipeline 61 is connected between the 32nd valve 53 and the 37th valve 59. A second connecting pipeline 62 is provided between the second input pipeline 26 and the second output pipeline 35, one end of the second connecting pipeline 62 is connected between the 19th valve 29 and the first valve array group 17, and the other end of the second connecting pipeline 62 is connected between the 33rd valve 54 and the 38th valve 60. The 39th valve 63 and the 40th valve 64 are provided on the first connecting pipeline 61 and the second connecting pipeline 62, respectively.

[0058] The bottom reflux port of the first RTF chromatography column 65 is connected to the third valve array group 67 through the first reflux port 30, and the bottom reflux port of the second RTF chromatography column 66 is connected to the third valve array group 68 through the second reflux port 31. The third valve array group 67 is a rectangular valve array formed by four valves connected at the end, as shown in the figure, the 41st valve 69, the 42nd valve 70, the 43rd valve 71, and the 44th valve 72 in the clockwise direction. The bottom reflux port of the first RTF chromatography column 65 is connected between the 41st valve 69 and the 44th valve 72; the bottom reflux port of the second RTF chromatography column 66 is connected between the 42nd valve 70 and the 43rd valve 71. One end of the filtration input main pipeline 73 is connected between the 43rd valve 71 and the 44th valve 72; the waste pipeline is connected between the 41st valve 69 and the 42nd valve 70. The other end of the filter input main pipeline 73 is connected to the fourth valve array group 74. The filter input main pipeline 73 is provided with a third input pump 75, a third flow meter 76, a third pressure sensor 77, and a forty-fifth valve 78 in sequence from right to left. The fourth valve array group 74 is a rectangular valve array formed by four valves connected at the end. As shown in the figure, the forty-sixth valve 79, the forty-seventh valve 80, the forty-eighth valve 81, and the forty-ninth valve 82 are connected in clockwise direction; the other end of the filter input main pipeline 73 is connected between the forty-seventh valve 80 and the forty-eighth valve 81; the forty-sixth valve 79 and the forty-ninth valve 82 are connected to the waste pipe. The bottom end of the first filter 83 is connected between the forty-sixth valve 79 and the forty-seventh valve 80, and the end of the second filter 84 is connected between the forty-eighth valve 81 and the forty-ninth valve 82. The tops of the first filter 83 and the second filter 84 are connected to the fifth valve array group 85 respectively. The fifth valve array group 85 is a rectangular valve array formed by connecting four valves at the end. As shown in the figure, the 50th valve 86, the 51st valve 87, the 52nd valve 88, and the 53rd valve 89 are connected in clockwise direction; the top of the first filter 83 is connected between the 50th valve 86 and the 51st valve 87, and the top of the second filter 84 is connected between the 52nd valve 88 and the 53rd valve 89. The outlet of the liquid input pipeline 90 is connected between the 50th valve 86 and the 53rd valve 89, and the inlet of the input pipeline 90 is connected to the hot WFI storage tank and the cold WFI storage tank respectively. The waste discharge pipeline and the first input main pipeline 1 are connected through pipelines between the 51st valve 87 and the 52nd valve 88, and the connection point is between the third valve 5 and the mixer 6. The 54th valve 91 and the 55th valve 92 are respectively set on the pipeline.

[0059] The first collecting pipe 41 is provided with a UV sensor 93, a first conductivity sensor 94, and a first pH sensor 95. The second collecting pipe 42 is provided with a second conductivity sensor 96 and a second pH sensor 97.

Claims

1. Fully automatic multifunctional pilot platform system, characterized by: The platform system includes a first input main pipeline with a first input pump and a second input main pipeline with a second input pump; the first input main pipeline is connected to multiple input pipelines and is controlled to be on and off by corresponding valves; the second input main pipeline is also respectively connected to the above-mentioned multiple input pipelines and is controlled to be on and off by corresponding valves; the outlet ends of the multiple input pipelines are respectively connected to the liquid inlet ports of the corresponding movable installation modules, and the movable installation modules are also provided with multiple reflux ports and multiple liquid outlet ports; the liquid outlet ports are respectively connected to multiple collection pipelines through output pipelines.

2. The fully automatic multifunctional pilot platform system according to claim 1, characterized in that: The movable installation module can be used to install a chromatography column.

3. The fully automatic multifunctional pilot platform system according to claim 1 is characterized in that: There are two input pipes; two corresponding liquid inlet ports; two reflux ports; two liquid outlet ports; and two output pipes.

4. The fully automatic multifunctional pilot platform system according to claim 3 is characterized in that: The first input main pipeline and the second input main pipeline are connected to the two input pipelines through a first valve array group; the first valve array group includes four valves connected end to end to form a rectangular valve array; one group of opposite sides of the rectangular valve array are respectively connected to the corresponding first input main pipeline and the second input main pipeline; the other group of opposite sides of the rectangular valve array are respectively connected to the corresponding two input pipelines.

5. The fully automatic multifunctional pilot platform system according to claims 1 and 4, characterized in that: There are two collecting pipes.

6. The fully automatic multifunctional pilot platform system according to claim 5, characterized in that: The two output pipes are connected to the two collecting pipes through a second valve array group; the second valve array group includes four valves connected end to end to form a rectangular valve array; one group of opposite sides of the rectangular valve array are respectively connected to the corresponding output pipes; the other group of opposite sides of the rectangular valve array are respectively connected to the corresponding two collecting pipes.

7. The fully automatic multifunctional pilot platform system according to claim 5, characterized in that: The outlet end of the second input main pipeline is connected to two collecting pipelines through two valves respectively.

8. The fully automatic multifunctional pilot platform system according to claim 5, characterized in that: One side of the output end of the second input pump of the second input main pipeline is connected to the first input main pipeline through a valve.

9. A fully automatic multifunctional chromatography system based on the pilot platform, characterized in that: The chromatography system comprises a plurality of RTF chromatography columns, wherein the inlet end at the bottom of each RTF chromatography column is connected to a corresponding liquid inlet port, and the reflux end at the bottom of each RTF chromatography column is connected to a corresponding reflux port; the outlet end at the top of each RTF chromatography column is connected to a liquid outlet port; each of the liquid inlet ports is connected to an outlet end of a corresponding input pipeline, and the inlet end of each input pipeline is respectively connected to a first input main pipeline with a first input pump and a second input main pipeline with a second input pump, and the on-off is controlled by a corresponding valve; each of the liquid outlet ports is respectively connected to an inlet end of a corresponding output pipeline, and the outlet end of each output pipeline is connected to a plurality of collection pipelines.

10. A fully automatic multifunctional chromatography system according to claim 9, characterized in that: An air trap is arranged on the first input main pipeline.

11. A fully automatic multifunctional chromatography system according to claim 9, characterized in that: The first input main pipeline is provided with a mixer.

12. The fully automatic multifunctional chromatography system according to claim 9, characterized in that: The RTF chromatography columns are provided with two.

13. A fully automatic multifunctional chromatography system according to claim 12, characterized in that: The first input main pipeline and the second input main pipeline are connected to the two input pipelines through a first valve array group; the first valve array group includes four valves connected end to end to form a rectangular valve array; one group of opposite sides of the rectangular valve array are respectively connected to the corresponding first input main pipeline and the second input main pipeline; the other group of opposite sides of the rectangular valve array are respectively connected to the corresponding two input pipelines.

14. A fully automatic multifunctional chromatography system according to claim 12, characterized in that: The input pipeline and the output pipeline in each RTF chromatography column are connected via a valve.

15. The fully automatic multifunctional chromatography system according to claim 9, characterized in that: There are two collecting pipes.

16. A fully automatic multifunctional chromatography system according to claim 15, characterized in that: The two output pipes are connected to the two collecting pipes through a second valve array group; the second valve array group includes four valves connected end to end to form a rectangular valve array; one group of opposite sides of the rectangular valve array are respectively connected to the corresponding output pipes; the other group of opposite sides of the rectangular valve array are respectively connected to the corresponding two collecting pipes.

17. A fully automatic multifunctional chromatography system according to claim 16, characterized in that: The second input main pipeline is connected to two collecting pipelines respectively through two valves.

18. The fully automatic multifunctional chromatography system according to claim 9, characterized in that: The inlet end of the second input main pipeline is connected to the first input main pipeline through a valve.

19. The fully automatic multifunctional chromatography system according to claim 9, characterized in that: The two reflux ports of the RTF chromatography column are respectively connected to corresponding reflux pipes, and the reflux pipes are respectively connected to the filtering modules.

20. The fully automatic multifunctional chromatography system according to claim 19, characterized in that: The two return pipes are connected to the filter module through the third valve array group. The third valve array group includes four valves connected end to end to form a rectangular valve array; one group of opposite sides of the rectangular valve array are respectively connected to the corresponding return pipes; one side of the other group of opposite sides of the rectangular valve array is connected to the filter module, and the other side is connected to the waste pipe.

21. A fully automatic multifunctional chromatography system according to claim 20, characterized in that: The filtering module includes a filtering input main pipeline, on which a third input pump is arranged, one end of the filtering input main pipeline is connected to the third valve array group, and the other end of the filtering input main pipeline is connected to the fourth valve array group, wherein the fourth valve array includes four valves connected end to end to form a rectangular valve array, and the filtering input main pipeline is connected to one side of a group of opposite sides of the rectangular valve array, wherein the other side is connected to a waste discharge pipeline; the other group of opposite sides of the matrix are respectively connected to one end of the corresponding filter; the other ends of the two filters are respectively connected to the fifth valve array group; the fifth valve array group includes four valves connected end to end to form a rectangular valve array; the other ends of the two filters are respectively connected to a group of opposite sides of the rectangular valve array; one side of the other group of opposite sides of the rectangular valve array is connected to the liquid input pipeline, and the other side of the other group of opposite sides of the rectangular valve array is connected to the first input main pipeline.

22. A fully automatic multifunctional chromatography system according to claim 21, characterized in that: One side of a group of opposite sides of the fifth valve array group is connected to the liquid input pipeline, and the other side of a group of opposite sides of the rectangular valve array group is connected to the first input main pipeline and the waste discharge pipeline.

Images