Negative pressure cleaning method for liquid injection and suction needles in cleaning disc

Abstract

The invention discloses a negative pressure cleaning method for liquid injection and suction needles in a cleaning disc. A cleaning tank in a negative pressure state is arranged at the top of the cleaning disc and located between a rotating disc and a needle body lifting mechanism. During normal work, the needle body lifting mechanism is controlled to drive the liquid injection needles and the liquid suction needles to move downwards, penetrate through the cleaning tank and are inserted into the reaction cups on the rotating disc to clean the reaction cups. When the liquid injection needle and the liquid suction needle need to be cleaned, the needle body lifting mechanism is controlled to drive the liquid injection needle and the liquid suction needle to move downwards, so that the liquid injection needle and the liquid suction needle are inserted into the cleaning tank to be cleaned. When the liquid injection and suction needle is cleaned, the length of the needle body of the liquid injection and suction needle and the downward moving stroke of the liquid injection and suction needle are shortened, and therefore the occurrence probability of factors influencing detection operation is reduced.

Description

Technical Field

[0001] The invention relates to a needle cleaning method, in particular to a negative pressure cleaning method for injection and aspiration needles of a cleaning disk in a full-automatic single molecule immunoassay device, belonging to the technical field of medical inspection. Background Art

[0002] Since the first automatic chemical analysis instrument was manufactured, more than half a century of development has gone by, and the fully automatic immunoanalyzer has become mature in technology. The current fully automatic immunoanalyzer has the following characteristics: 1. A multi-degree-of-freedom robotic arm is used to coordinate the actions between various modules; 2. The instrument has extremely strong flexibility and can meet various analysis needs; 3. The test speed is fast and the continuous operation time is long without human intervention; 4. The combination of multiple technologies and complete automation of processing in the detection technology make the test results more accurate and more precise. The fully automatic immunoanalyzer can realize the steps of taking the reaction cup, adding specimens, adding reaction liquid, shaking, promoting reaction, measuring, calculating and analyzing, and cleaning in the experimental test process. It replaces manual operation, which not only saves labor costs, but more importantly, eliminates human errors and ensures the accuracy of the data. The fully automatic immunoanalyzer has the advantages of fast, efficient, high precision, and repeatable consistency. It has been widely used in processing, production, testing, life assistance and other fields, and it is bound to become a trend in the field of medical testing.

[0003] During the test, nanomagnetic beads need to be combined with antibodies and antigens. After the nanomagnetic beads are combined with antibodies and antigens, the remaining liquid in the reaction cup needs to be cleaned to ensure the detection effect. The principle of magnetic separation and cleaning is: in the principle of enzymatic chemiluminescent immunoassay technology, the cloned antibodies in the magnetic bead particle reagent and the substances to be detected in the sample, as well as the enzyme-labeled antibodies or antigens in the reagent, form magnetic immune complexes that can be suspended in the reaction system after non-specific immune reactions. In the magnetic field formed by the magnet, the magnetic immune complex and the unbound magnetic particles are quickly captured by the magnetic field and attached to the part close to the magnetic field, and the liquid outside the magnetic particle complex is removed, thereby separating the magnetic particle complex from the enzyme particles in the liquid. When the external magnetic field is removed and the cleaning solution is injected, the magnetic particle complex can be evenly dispersed in the solution, and the free enzyme is cleaned multiple times. As a reaction carrier of antigen and antibody, the suspendable characteristics of magnetic beads enable the immune reaction to occur quickly under uniform conditions, while promoting the rapid separation of immune complexes from the solution.

[0004] In the immunoassay analyzer, the cleaning tray is responsible for cleaning the reaction cup. During the cleaning process, the reaction cup must repeatedly go through the process of "liquid injection cleaning-liquid aspiration and discharge" until it is sent to the next process for testing. In the prior art, there is a problem that when the machine is stopped for a period of time, the injection needle and the liquid aspiration needle in the cleaning tray need to be cleaned. The cleaning tray in the prior art can only use the injection and liquid aspiration needles to clean the magnetic beads in the reaction cup. When the injection needle and the liquid aspiration needle themselves are to be cleaned, the operation steps of the cleaning method are very cumbersome and the cleaning effect is not good.

[0005] The applicant submitted an invention patent application in 2023, with publication number CN116984298A and publication date November 3, 2023. The patent document discloses a method for cleaning the injection and aspiration needles in the cleaning tray of an immunoassay analyzer, wherein a cleaning tank is arranged inside the cleaning tray below the turntable; during normal operation, the injection needle and the aspiration needle are controlled to move downward and inserted into the reaction cup on the turntable to clean the reaction cup; when the injection and aspiration needles need to be cleaned, the reaction cup is first removed, and then the injection needle and the aspiration needle are controlled to move downward, so that the injection needle and the aspiration needle pass through the turntable and are inserted into the cleaning tank to clean the injection needle and the aspiration needle.

[0006] In this patent document, the cleaning tank is located at the lower part of the cleaning tray. When cleaning the injection needle and the pipette needle, the injection needle and the pipette needle need to be controlled to move downward and inserted into the cleaning tank for cleaning. Therefore, the needle body length of the injection and pipette needles and the downward movement stroke of the injection and pipette needles are relatively long, which may cause some problems affecting the detection operation.

[0007] Therefore, how to redesign a cleaning method for the injection and pipette needles in the cleaning plate so that the needle body length and the downward movement stroke of the injection and pipette needles can be shortened during cleaning, thereby reducing the probability of occurrence of factors affecting the detection operation is a technical problem that needs to be solved urgently. Summary of the invention

[0008] The technical problem to be solved by the present invention is to address the defects existing in the prior art and to provide a negative pressure cleaning method for the injection and pipette needles in a cleaning tray. When the injection and pipette needles are cleaned, the needle body length and the downward movement stroke of the injection and pipette needles can be shortened, thereby reducing the probability of occurrence of factors affecting the detection operation.

[0009] In order to solve the above technical problems, the technical solution adopted by the present invention is: a negative pressure cleaning method for injection and pipetting needles in a cleaning disk, which is to set a cleaning tank in a negative pressure state on the top of the cleaning disk and between the turntable and the needle body lifting mechanism; when working normally, the needle body lifting mechanism is controlled to drive the injection needle and the pipetting needle to move downward through the cleaning tank and inserted into the reaction cup on the turntable to clean the reaction cup; when the injection and pipetting needles need to be cleaned, the needle body lifting mechanism is controlled to drive the injection needle and the pipetting needle to move downward, so that the injection needle and the pipetting needle are inserted into the cleaning tank for cleaning the injection needle and the pipetting needle.

[0010] Preferably, one end of the cleaning tank is connected to pump one through a water inlet joint, and the other end of the cleaning tank is connected to pump two through a water outlet joint. Pump one is used to deliver cleaning water and air into the cleaning tank, and pump two is used to extract the cleaning water and air in the cleaning tank, so that negative pressure can be formed in the cleaning tank through pump two; through the cooperation of pump one and pump two, the cleaning water circulates between the cleaning tank and the outside world.

[0011] Preferably, pairs of through holes are provided on the cleaning tank at positions corresponding to the injection needle and the aspiration needle, and valves are provided in the upper and lower holes of the pairs of through holes; and a barrel through hole is provided on the top of the cleaning tray at the position of each pair of through holes; When working normally, the injection needle and the suction needle are inserted into the reaction cup after penetrating the valve in the upper hole, the valve in the lower hole and the cylinder through hole on the top of the cleaning plate through the cleaning tank.

[0012] Preferably, the cleaning method of the injection and aspiration needles includes an injection needle cleaning method and an aspiration needle cleaning method, and the specific steps of the injection needle cleaning method are: When negative pressure is formed in the cleaning tank: 1) Control the needle lifting mechanism to descend, so that the injection needle is inserted into the cleaning tank from the valve of the perforated upper hole of the cleaning tank, and then stop the needle lifting mechanism; 2) The injection needle is controlled to start injecting cleaning water, which is then injected into the cleaning tank. Finally, the cleaning water is drawn away through the water outlet joint, thereby using the cleaning water flowing through the injection needle to clean the inner wall of the injection needle.

[0013] Preferably, the liquid-absorbing needle cleaning method includes a needle body inner wall cleaning method and a needle body outer wall cleaning method, and the specific steps of the needle body inner wall cleaning method of the liquid-absorbing needle are: When negative pressure is formed in the cleaning tank: A1: Control the needle lifting mechanism to descend, so that the liquid-absorbing needle is inserted from the valve of the perforated upper hole of the cleaning tank into the cleaning water in the cleaning tank, and then stop the needle lifting mechanism; A2: Control the aspiration needle to start absorbing the cleaning water in the cleaning tank. Part of the cleaning water is sucked away by the aspiration needle, and the other part of the cleaning water is drawn out through the water outlet joint, so that the cleaning water flowing through the aspiration needle is used to clean the inner wall of the aspiration needle.

[0014] Preferably, the specific steps of the method for cleaning the outer wall of the needle body of the liquid-absorbing needle are: When negative pressure is formed in the cleaning tank: B1: Control the needle lifting mechanism to descend, so that the aspiration needle is inserted into the cleaning tank from the valve of the upper hole of the cleaning tank pair of perforations, and then continues to pass through the valve of the lower hole of the cleaning tank pair of perforations from the cleaning tank; B2: Then control the needle lifting mechanism to rise, driving the liquid aspiration needle from the valve of the perforated lower hole of the cleaning tank back to the cleaning tank; B3: Then repeat steps B1 and B2 until the outer wall of the needle body is cleaned; After the outer wall of the needle body is cleaned, the needle body lifting mechanism is controlled to rise, driving the liquid-absorbing needle to be drawn out from the upper hole of the perforation on the cleaning tank.

[0015] Preferably, the specific steps of the method for cleaning the inner wall of the needle body of the aspiration needle and the specific steps of the method for cleaning the outer wall of the needle body of the aspiration needle are performed separately.

[0016] Preferably, when executing the specific steps of the method for cleaning the outer wall of the needle body of the pipette needle, the length of the outer wall of the pipette needle to be cleaned is controlled by controlling the up and down stroke of the pipette needle.

[0017] The beneficial effects of the present invention are as follows: by moving the cleaning tank upward, the present invention shortens the needle body length of the injection and aspiration needle and the downward movement stroke of the injection and aspiration needle when performing the cleaning operation of the injection and aspiration needle itself, compared with the prior art, thereby reducing the probability of occurrence of factors affecting the detection operation; in addition, by designing the cleaning tank to be in a negative pressure state, when the injection and aspiration needles pass through the cleaning tank, there will be no problem of cleaning water leakage, thereby ensuring the normal progress of the detection operation. By designing specific cleaning steps for the injection and aspiration needles, the cleaning steps are further simplified, the convenience of cleaning the injection and aspiration needles is improved, and the cleaning effect of the injection and aspiration needles is improved. When cleaning the outer wall of the needle body of the aspiration needle, the length of the cleaning of the outer wall of the aspiration needle is controlled by controlling the up and down lifting stroke of the aspiration needle, so that the present invention can select the length of the cleaning of the outer wall of the aspiration needle according to the actual working conditions, which is more practical. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a schematic diagram of the axial cross-sectional structure of the cleaning disk in an embodiment of the present invention; Figure 2 Schematic diagram of the three-dimensional structure of the cleaning tank in the cleaning tray of the embodiment of the present invention Figure 1 ; Figure 3 Schematic diagram of the three-dimensional structure of the cleaning tank in the cleaning tray of the embodiment of the present invention Figure 2 ; Figure 4 for Figure 2 A schematic diagram of the enlarged structure of the middle part A; Figure 5 for Figure 1 A schematic diagram of a partial axial cross-sectional structure located at a through hole of a cylinder; Figure 6 This is a schematic diagram of a partial axial cross-sectional structure of a cleaning plate in an embodiment of the present invention when an injection needle is inserted into a reaction cup; Figure 7 It is a schematic diagram of a partial axial cross-sectional structure of a cleaning disk in an embodiment of the present invention when cleaning an injection needle; Figure 8 It is a schematic diagram of a partial axial cross-sectional structure when the inner wall of the liquid pipetting needle is cleaned in the cleaning plate of the embodiment of the present invention; Fig. 9 The schematic diagram of the partial axial cross-sectional structure of the cleaning plate of the embodiment of the present invention when cleaning the outer wall of the liquid pipetting needle is shown in FIG. Figure 1 ; Fig.10 The schematic diagram of the partial axial cross-sectional structure of the cleaning plate of the embodiment of the present invention when cleaning the outer wall of the liquid pipetting needle is shown in FIG. Figure 2 .

[0019] In the figure: 1. cleaning disk cylinder, 111. cylinder through hole, 112. cleaning disk partition, 2. turntable mechanism, 211. turntable, 212. turntable motor and reducer, 3. needle lifting mechanism, 4. reaction cup, 5. cleaning tank, 511. tank body, 512. tank body flange, 513. cleaning chamber, 6. injection needle, 7. aspiration needle, 8. through hole, 811. upper hole, 812. lower hole, 9. valve, 10. water inlet connector, 11. water outlet connector. DETAILED DESCRIPTION

[0020] The technical solution of the present invention is further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0021] In order to shorten the needle body length of the injection and pipetting needles and the downward stroke of the injection and pipetting needles, the applicant moved the original cleaning tank from the lower part of the cleaning tray to the top of the cleaning tray, and cleaned the needle body in the cleaning tank on the top of the cleaning tray. This shortened the needle body length of the injection and pipetting needles and the downward stroke of the injection and pipetting needles. However, after moving to the top of the cleaning tray, the cleaning tank is located between the injection and pipetting needles and the reaction cup. When performing the detection operation, the injection and pipetting needles located at the upper position need to be moved downward and inserted into the reaction cup, and at this time the cleaning tank is located between the injection and pipetting needles and the reaction cup. When the injection and pipetting needles move downward, they need to pass through the cleaning tank to be inserted into the reaction cup, and there is cleaning water in the cleaning tank. Therefore, the cleaning tank needs to be improved to ensure that the cleaning water does not leak out when the injection and pipetting needles pass through the cleaning tank.

[0022] Example: Figure 1 As shown, the cleaning disk includes a cleaning disk cylinder 1, a turntable mechanism 2 disposed inside the cleaning disk cylinder 1, and a needle lifting mechanism 3 disposed above the cleaning disk cylinder 1; a reaction cup 4 is placed on a turntable 211 of the turntable mechanism 2, and the turntable 211 can drive the reaction cup 4 to rotate. A closed cleaning tank 5 is disposed on the top of the cleaning disk cylinder 1 and below the needle lifting mechanism 3, and both the injection needle 6 and the aspiration needle 7 are disposed on the needle lifting mechanism 3. Figure 2 and Figure 3 As shown, since the injection needles and the aspiration needles are distributed in the circumferential direction of the turntable, the closed cleaning tank 5 is correspondingly arranged in an arc shape, and a pair of perforations 8 are arranged on the cleaning tank 5 and below each injection needle 6 and aspiration needle 7. In this embodiment, three injection needles 6 and three aspiration needles 7 are arranged, so a total of six pair of perforations 8 are arranged in the cleaning tank 5. The pair of perforations 8 include an upper hole 811 arranged on the top surface of the cleaning tank 5 and a lower hole 812 arranged on the bottom surface of the cleaning tank 5, as shown in FIG. Figure 4 As shown, a valve 9 is provided at the opening of each upper hole 811 and lower hole 812. The valve 9 is made of silicone material and has a cross slit in the middle, thereby forming a petal shape to facilitate the needle to pass through. Figure 2 and Figure 3As shown, a water inlet joint 10 is provided on one end of the cleaning tank 5, and a water outlet joint 11 is provided on the other end of the cleaning tank 5. The water inlet joint 10 is connected to pump 1 (not shown in the figure), and the water outlet joint 11 is connected to pump 2 (not shown in the figure). Pump 1 is used to deliver cleaning water and air into the cleaning tank 5, and pump 2 is used to extract the cleaning water and air in the cleaning tank 5. Pump 2 can form a certain negative pressure in the cleaning tank 5, so that through the cooperation of the above-mentioned pump 1 and pump 2, the cleaning water circulates between the cleaning tank 5 and the outside world and a certain negative pressure state is formed in the cleaning tank 5. The negative pressure state and the cooperation between the needle body and the valve can prevent the cleaning water from flowing out of the cleaning tank 5. It should be noted here that the cleaning tank 5 is not completely filled with cleaning water. Since the outside air will also be delivered into the cleaning tank 5, the cleaning tank 5 is in a mixed state of air and cleaning liquid. In addition, as Figure 5 As shown, in order to facilitate the needle to be inserted into the reaction cup, a cylinder through hole 111 is opened on the top of the cleaning disk cylinder 1 and at the position of each through hole 8.

[0023] like Figure 6 As shown, the cleaning method of the injection and aspiration needles in the cleaning disk is that during normal detection work, driven by the downward movement of the needle body lifting mechanism, the injection needle 6 and the aspiration needle 7 can descend and successively pass through the valve 9 of the upper hole 811 of the perforation, the valve 9 of the lower hole 812 of the perforation and the cylinder through hole 111 on the top of the cleaning disk cylinder 1, and then be inserted into the reaction cup 4 to clean the reaction cup; when it is necessary to clean the injection and aspiration needles, it is only necessary to control the downward movement of the needle body lifting mechanism so that the injection needle and the aspiration needle are inserted from the perforations on the cleaning tank into the cleaning tank for cleaning.

[0024] When the needle passes through the valve, a sealing structure is formed between the needle and the valve. Since the cleaning water in the cleaning tank flows rapidly and the cleaning tank is in a negative pressure state, the cleaning water in the cleaning tank will not leak out from the valve, thereby avoiding the occurrence of cleaning water leakage.

[0025] Therefore, this embodiment moves the cleaning tank upward. Compared with the prior art, when performing the cleaning operation of the injection and pipetting needles themselves, the needle body length of the injection and pipetting needles and the downward movement stroke of the injection and pipetting needles are shortened, thereby reducing the probability of occurrence of factors affecting the detection operation; in addition, by designing the cleaning tank to be in a negative pressure state, when the injection and pipetting needles pass through the cleaning tank, there will be no problem of cleaning water leakage, thereby ensuring the normal progress of the detection operation.

[0026] Therefore, if Figure 2 and Figure 5As shown, the cleaning tank 5 includes a fully enclosed arc-shaped tank body 511 and a tank body flange 512 arranged on one side of the arc-shaped tank body 511. The cleaning tank 5 is screwed into the top of the cleaning disc cylinder body 1 through the tank body flange 512 by installing screws (not shown in the figure), so that the cleaning tank 5 is arranged on the top of the cleaning disc cylinder body 1. A cleaning cavity 513 is arranged in the arc-shaped tank body 511, and the water inlet joint 10 and the water outlet joint 11 are respectively arranged at both ends of the arc-shaped tank body 511, and the water inlet joint 10 and the water outlet joint 11 are both connected to the cleaning cavity 513. The upper hole 811 is arranged at the top of the arc-shaped tank body 511, and the lower hole 812 is arranged at the bottom of the arc-shaped tank body 511. The upper hole 811 and the lower hole 812 are both connected to the cleaning cavity 513. The upper hole 811 and the lower hole 812 are both arranged as countersunk holes, and the valve 9 is arranged in the countersunk upper hole 811 and the lower hole 812. Since the volume of the cleaning tank 5 in this embodiment is relatively small, compared with the cleaning tank in the patent document mentioned in the background technology, the volume is two-thirds smaller, and the above-mentioned setting makes the volume of the cleaning chamber 513 even smaller, so that it is easier to form a negative pressure state in the cleaning chamber 513 with a smaller volume. In addition, the cleaning tank in the prior art is provided with multiple water inlets, while the cleaning tank in this embodiment is only provided with a one-in-one-out structure, so that the structure of the cleaning tank in this embodiment is simpler. The cleaning tank in the prior art is installed inside the cleaning disc cylinder body, which is very inconvenient when the cleaning tank needs to be disassembled, while the cleaning tank in this embodiment is installed on the top of the cleaning disc cylinder body 1 by screws, located outside the cleaning disc cylinder body 1, so that it is easier to disassemble and assemble the cleaning tank.

[0027] In daily work, when the cleaning tray cleans the magnetic beads in the reaction cup, when the reaction cup is filled with liquid by the injection needle, since the injection needle is inserted into the liquid in the reaction cup, when the injection needle is cleaned, only the inner wall of the injection needle needs to be cleaned; and when the reaction cup is filled with liquid by the suction needle, since the suction needle needs to be inserted into the liquid in the reaction cup, when the suction needle is cleaned, both the inner wall and the outer wall of the suction needle need to be cleaned. In the prior art, when cleaning the injection needle and the suction needle, the needle body is inserted into the cleaning tank and then kept stationary for cleaning. In this way, especially when cleaning the outer wall of the suction needle, only a very small distance can be cleaned, and the cleaning effect is not very good.

[0028] In this embodiment, since the position and structure of the cleaning tank are changed, the cleaning steps are also changed. The cleaning method of the injection and aspiration needles in this embodiment includes an injection needle cleaning method and an aspiration needle cleaning method. The specific steps of the injection needle cleaning method are as follows: When negative pressure is formed in the cleaning chamber 513 of the cleaning tank: 1) If Figure 7As shown, the needle lifting mechanism is controlled to descend, so that the injection needle 6 is inserted into the cleaning chamber 513 from the upper hole 811 perforated on the cleaning tank, and then the needle lifting mechanism is stopped; 2) Control the injection needle 6 to start injecting cleaning water, the cleaning water is injected into the cleaning chamber 513, and finally the cleaning water is pumped away through the water outlet joint, so that the cleaning water flowing through the injection needle 6 is used to clean the inner wall of the injection needle 6, thereby improving the cleaning effect.

[0029] The liquid-absorbing needle cleaning method includes a needle inner wall cleaning method and a needle outer wall cleaning method. The specific steps of the needle inner wall cleaning method of the liquid-absorbing needle are as follows: When negative pressure is formed in the cleaning chamber 513 of the cleaning tank: A1: Figure 8 As shown, the needle lifting mechanism is controlled to descend, so that the liquid-absorbing needle 7 is inserted into the cleaning water in the cleaning chamber 513 from the upper hole 811 perforated on the cleaning tank, and then the needle lifting mechanism is stopped; A2: Control the liquid-absorbing needle 7 to start absorbing cleaning water. Part of the cleaning water is absorbed by the liquid-absorbing needle 7, and the other part of the cleaning water is drawn out through the water outlet joint, so that the cleaning water flowing through the liquid-absorbing needle 7 is used to clean the inner wall of the liquid-absorbing needle 7.

[0030] The specific steps of the method for cleaning the outer wall of the liquid-absorbing needle are as follows: When negative pressure is formed in the cleaning chamber 513 of the cleaning tank: B1: If Fig. 9 and Fig.10 As shown, the needle lifting mechanism is controlled to descend, so that the liquid suction needle 7 is inserted into the cleaning chamber 513 from the upper hole 811 of the cleaning tank, and then continues to pass through the lower hole 812 of the cleaning tank from the cleaning chamber 513; B2: Then the needle lifting mechanism is controlled to rise, driving the liquid aspiration needle 7 to return from the lower hole 812 perforated on the cleaning tank to the cleaning chamber 513; B3: Then repeat steps B1 and B2 until the outer wall of the needle body is cleaned; After the outer wall of the needle body is cleaned, the needle body lifting mechanism is controlled to rise, driving the liquid-absorbing needle 7 to be drawn out from the upper hole 811 perforated on the cleaning tank.

[0031] It should be noted that, in the present embodiment, the cleaning of the inner and outer walls of the aspiration needle are generally carried out separately. This is because when cleaning the outer wall of the needle body, the aspiration needle needs to be controlled to stop aspirating liquid. This can prevent part of the cleaning water and air in the cleaning tank from being sucked away by the aspiration needle during the up and down movement of the aspiration needle, resulting in the problem of unstable negative pressure in the cleaning tank.

[0032] When cleaning the outer wall of the pipette needle, the present embodiment is carried out by controlling the pipette needle to rise and fall repeatedly in coordination with the cleaning water in the cleaning tank. In this way, the length of the cleaning of the outer wall of the pipette needle can be determined by controlling the stroke of the pipette needle to rise and fall. This allows the present embodiment to select the length of the cleaning of the outer wall of the pipette needle according to actual working conditions, which is more practical.

[0033] like Figure 1 As shown, a cleaning disk partition 112 is provided inside the cleaning disk cylinder 1, and the cleaning disk partition 112 divides the inside of the cleaning disk cylinder 1 into an upper cylinder space located at an upper position and a lower cylinder space located at a lower position. The turntable 211 of the turntable mechanism 2 is located in the upper cylinder space, and a heat preservation material and a heating device (not shown in the figure) are provided inside the upper cylinder space, and the heating device adopts an electric heating film. The interior of the upper cylinder space is heated and maintained at a certain temperature by the heat preservation material and the heating device, because a certain working temperature needs to be maintained after the reaction cup is placed on the turntable. In this embodiment, the turntable is arranged in the heat preservation space of the upper cylinder space, so that the reaction cup on the turntable can always be in a certain working temperature environment.

[0034] The turntable mechanism 2 further includes a turntable motor and reducer 212, which are arranged on the cleaning disk partition 112, and a rotating shaft 6121 of the turntable motor and reducer 612 extends into the upper cylinder space and is connected to the turntable 211. In the prior art, the turntable is driven by a belt transmission, so that the transmission structure is relatively complicated and the space occupied by the transmission mechanism is relatively large. In this embodiment, the turntable motor integrated with the reducer is directly connected to the turntable, and the belt transmission structure is cancelled, thereby simplifying the structure of the rotating mechanism and reducing the occupied space.

[0035] In summary, the present invention moves the cleaning tank upwards, and compared with the prior art, when performing the cleaning operation of the injection and aspiration needle itself, the needle body length of the injection and aspiration needle and the downward movement stroke of the injection and aspiration needle are shortened, thereby reducing the probability of occurrence of factors affecting the detection operation; in addition, by designing the cleaning tank to be in a negative pressure state, when the injection and aspiration needle pass through the cleaning tank, there will be no problem of cleaning water leakage, ensuring the normal progress of the detection operation. By designing specific cleaning steps for the injection and aspiration needle, the cleaning steps are further simplified, the convenience of cleaning the injection and aspiration needle is improved, and the cleaning effect of the injection and aspiration needle is improved. When cleaning the outer wall of the needle body of the aspiration needle, the length of the cleaning of the outer wall of the aspiration needle is controlled by controlling the up and down lifting stroke of the aspiration needle, so that the present invention can select the length of the cleaning of the outer wall of the aspiration needle according to the actual working conditions, which is more practical.

[0036] The "multiple" mentioned in this embodiment refers to the number of "two or more". The above embodiments are only used to illustrate the present invention, but not to limit the present invention. Those skilled in the relevant technical field can make various changes or modifications without departing from the spirit and scope of the present invention. Therefore, all equivalent technical solutions should also belong to the protection scope of the present invention, and the protection scope of the present invention should be defined by the claims.

Claims

1. A negative pressure cleaning method for cleaning the injection and aspiration needles in a cleaning plate, which is characterized by: A cleaning tank in a negative pressure state is arranged on the top of the cleaning disk and between the rotating disk and the needle body lifting mechanism; during normal operation, the needle body lifting mechanism is controlled to drive the injection needle and the liquid suction needle to move downward through the cleaning tank and insert into the reaction cup on the rotating disk to clean the reaction cup; when the injection and liquid suction needles need to be cleaned, the needle body lifting mechanism is controlled to drive the injection needle and the liquid suction needle to move downward, so that the injection needle and the liquid suction needle are inserted into the cleaning tank for cleaning.

2. The negative pressure cleaning method according to claim 1, characterized in that: One end of the cleaning tank is connected to pump one through a water inlet joint, and the other end of the cleaning tank is connected to pump two through a water outlet joint. Pump one is used to deliver cleaning water and air into the cleaning tank, and pump two is used to extract the cleaning water and air in the cleaning tank, so that negative pressure can be formed in the cleaning tank through pump two; through the cooperation of pump one and pump two, the cleaning water circulates between the cleaning tank and the outside world.

3. The negative pressure cleaning method according to claim 2, characterized in that: Pairs of through holes are provided on the cleaning tank at positions corresponding to the injection needle and the aspiration needle, and valves are provided in the upper and lower holes of the pair of through holes; a barrel through hole is provided on the top of the cleaning tray at the position of each pair of through holes; When working normally, the injection needle and the suction needle are inserted into the reaction cup after penetrating the valve in the upper hole, the valve in the lower hole and the cylinder through hole on the top of the cleaning plate through the cleaning tank.

4. The negative pressure cleaning method according to claim 3, characterized in that: The cleaning method of the injection and aspiration needle includes a cleaning method of the injection needle and a cleaning method of the aspiration needle. The specific steps of the cleaning method of the injection needle are as follows: When negative pressure is formed in the cleaning tank: 1) Control the needle lifting mechanism to descend, so that the injection needle is inserted into the cleaning tank from the valve of the perforated upper hole of the cleaning tank, and then stop the needle lifting mechanism; 2) The injection needle is controlled to start injecting cleaning water, which is then injected into the cleaning tank. Finally, the cleaning water is drawn away through the water outlet joint, thereby using the cleaning water flowing through the injection needle to clean the inner wall of the injection needle.

5. The negative pressure cleaning method according to claim 4, characterized in that: The liquid-absorbing needle cleaning method includes a needle body inner wall cleaning method and a needle body outer wall cleaning method. The specific steps of the liquid-absorbing needle inner wall cleaning method are as follows: When negative pressure is formed in the cleaning tank: A1: Control the needle lifting mechanism to descend, so that the liquid-absorbing needle is inserted from the valve of the perforated upper hole of the cleaning tank into the cleaning water in the cleaning tank, and then stop the needle lifting mechanism; A2: Control the aspiration needle to start absorbing the cleaning water in the cleaning tank. Part of the cleaning water is sucked away by the aspiration needle, and the other part of the cleaning water is drawn out through the water outlet joint, so that the cleaning water flowing through the aspiration needle is used to clean the inner wall of the aspiration needle.

6. The negative pressure cleaning method according to claim 5, characterized in that: The specific steps of the method for cleaning the outer wall of the needle body of the liquid-absorbing needle are as follows: When negative pressure is formed in the cleaning tank: B1: Control the needle lifting mechanism to descend, so that the liquid suction needle is inserted into the cleaning tank from the valve of the upper hole of the cleaning tank, and then continues to pass through the valve of the lower hole of the cleaning tank from the cleaning tank; B2: Then control the needle lifting mechanism to rise, driving the liquid aspiration needle from the valve of the perforated lower hole of the cleaning tank back to the cleaning tank; B3: Then repeat steps B1 and B2 until the outer wall of the needle body is cleaned; After the outer wall of the needle body is cleaned, the needle body lifting mechanism is controlled to rise, driving the liquid-absorbing needle to be drawn out from the upper hole of the perforation on the cleaning tank.

7. The negative pressure cleaning method according to claim 6, characterized in that: The specific steps of the method for cleaning the inner wall of the needle body of the aspiration needle and the specific steps of the method for cleaning the outer wall of the needle body of the aspiration needle are performed separately.

8. The negative pressure cleaning method according to claim 6, characterized in that: When executing the specific steps of the method for cleaning the outer wall of the liquid-absorbing needle, the length of the outer wall of the liquid-absorbing needle to be cleaned is controlled by controlling the upward and downward stroke of the liquid-absorbing needle.

Images