Method for improving mycelium yield, polysaccharide yield and polysaccharide efficacy in liquid fermentation of pilose antler mushrooms

Abstract

The invention discloses a method for improving mycelium yield, polysaccharide yield and polysaccharide efficacy in liquid fermentation of pilose antler mushrooms, and belongs to the technical field of liquid fermentation of pilose antler mushrooms. The radix astragali aqueous extract is added into the pilose antler mushroom liquid fermentation culture medium, so that the growth speed of pilose antler mushroom hyphae is effectively promoted, the hypha yield is increased, the liquid fermentation time is shortened, meanwhile, the yield and titer of pilose antler mushroom polysaccharides can be increased, and the problems that the pilose antler mushroom liquid fermentation time is long, and the fermentation effect is poor are solved. The efficiency of extracting the antler mushroom polysaccharide from the sporocarp is relatively low; the functional titer of the antler mushroom polysaccharide is relatively low; and the development of the antler mushroom planting industry is limited.

Description

Technical Field

[0001] The invention relates to the technical field of liquid fermentation of velvet antler mushroom, and in particular to a method for improving mycelium yield, polysaccharide yield and polysaccharide efficacy in liquid fermentation of velvet antler mushroom. Background Art

[0002] Porcupine is a delicious edible mushroom, with the scientific name of Lycopodiella cernua. The fruiting body of Porcupine is crispy and delicious, rich in protein, vitamins and other nutrients. Porcupine polysaccharides have anti-tumor, immune function enhancement, antibacterial, blood sugar and lipid lowering effects, and have broad prospects for biomedical development and health food applications.

[0003] The cultivation techniques of Pleurotus eryngii include solid culture and liquid culture. However, solid culture has the disadvantages of long germination time, long seed production cycle, and high contamination rate, while the growth rate of liquid culture strains is fast, a certain biomass can be reached in a short time, and the culture method is simple and easy to control. Therefore, liquid culture is more suitable for industrial production, which is convenient for forming a stable Pleurotus eryngii industry. However, the existing Pleurotus eryngii liquid culture method still has problems such as long fermentation time, low Pleurotus eryngii polysaccharide yield and extraction efficiency, and low functional titer of Pleurotus eryngii polysaccharide. Therefore, providing a method that can accelerate the liquid fermentation of Pleurotus eryngii and increase the mycelium yield and polysaccharide yield is of great significance to promoting the development of the Pleurotus eryngii industry. Summary of the invention

[0004] The purpose of the present invention is to provide a method for increasing mycelium yield, polysaccharide yield and polysaccharide efficacy in liquid fermentation of Porcupine antler mushroom, so as to solve the problems existing in the above-mentioned prior art. The present invention effectively promotes the growth rate of Porcupine antler mushroom mycelium, increases mycelium yield and shortens liquid fermentation time by adding astragalus water extract to the liquid fermentation medium of Porcupine antler mushroom, and at the same time can also increase the yield and potency of Porcupine antler mushroom polysaccharide.

[0005] To achieve the above object, the present invention provides the following solutions:

[0006] The invention provides a pilose antler mushroom liquid fermentation culture medium, which comprises the following components in concentrations: 200 g / L potato, 8 g / L peptone, 20 g / L glucose, 1 g / L KH2PO4, 0.5 g / L MgSO4, 10 mg / L VB1, and 100-1000 mg / L astragalus water extract.

[0007] In the present invention, the astragalus water extract is obtained by extraction at room temperature, decoction, reflux and steam distillation using water as solvent; in some embodiments of the present invention, the astragalus water extract is obtained by extraction by decoction using water as solvent; specifically, the preparation method of the astragalus water extract comprises the following steps:

[0008] The astragalus powder was mixed with water in a ratio of 100 g: 1000 mL, decocted twice, the decoctions were combined, the decoctions were rotary evaporated at 45° C., and freeze-dried to obtain the astragalus water extract.

[0009] The present invention also provides an application of the above-mentioned Pleurotus eryngii liquid fermentation culture medium in promoting Pleurotus eryngii liquid fermentation.

[0010] Preferably, the promoting of liquid fermentation of Pleurotus eryngii includes increasing the mycelium yield in the liquid fermentation of Pleurotus eryngii, increasing the polysaccharide yield in the liquid fermentation of Pleurotus eryngii, increasing the polysaccharide efficacy in the liquid fermentation of Pleurotus eryngii and / or shortening the liquid fermentation time of Pleurotus eryngii.

[0011] The present invention also provides a method for increasing the mycelium yield in the liquid fermentation of Pleurotus eryngii, comprising the step of inoculating the liquid spawn of Pleurotus eryngii into the liquid fermentation medium of Pleurotus eryngii for fermentation and culturing to increase the mycelium yield in the liquid fermentation of Pleurotus eryngii.

[0012] The present invention also provides a method for increasing the polysaccharide yield in the liquid fermentation of Pleurotus eryngii, comprising the step of inoculating the liquid strain of Pleurotus eryngii into the above-mentioned liquid fermentation medium of Pleurotus eryngii for fermentation and culturing to increase the polysaccharide yield in the liquid fermentation of Pleurotus eryngii.

[0013] The present invention also provides a liquid fermentation method for improving the efficacy of Pleurotus eryngii polysaccharides, comprising the step of inoculating Pleurotus eryngii liquid strains into the above-mentioned Pleurotus eryngii liquid fermentation medium for fermentation and culturing to improve the efficacy of Pleurotus eryngii polysaccharides.

[0014] The present invention also provides a method for shortening the liquid fermentation time of velvet antler mushroom, comprising the step of inoculating velvet antler mushroom liquid strains into the above-mentioned velvet antler mushroom liquid fermentation medium for fermentation and culturing to shorten the liquid fermentation time of velvet antler mushroom.

[0015] Preferably, the fermentation culture temperature is 25°C, the rotation speed is 120r / min, and the time is 7-11d.

[0016] The present invention discloses the following technical effects:

[0017] The invention effectively promotes the growth rate of Pleurotus eryngii mycelium, increases the mycelium yield and shortens the liquid fermentation time by adding the astragalus water extract to the liquid fermentation medium of Pleurotus eryngii, and can also increase the yield and potency of Pleurotus eryngii polysaccharides, thereby solving the problems of long liquid fermentation time of Pleurotus eryngii, low efficiency of extracting Pleurotus eryngii polysaccharides from fruiting bodies, low functional potency of Pleurotus eryngii polysaccharides, and limited development of the Pleurotus eryngii planting industry.

[0018] According to experimental detection, the use of the velvet antler mushroom liquid fermentation culture medium of the present invention can shorten the velvet antler mushroom liquid fermentation cycle, shortening the production time by 12.5% ​​compared with the prior art; increasing the yield of velvet antler mushroom mycelium, entering the stable period after 7 days of cultivation, with the maximum mycelium biomass of 35.51 g / L, increasing the yield by 8.23% compared with the prior art; increasing the yield of velvet antler mushroom polysaccharide, with the crude polysaccharide content of velvet antler mushroom being 9.21 g / L, increasing the yield by 21% compared with the prior art; improving the efficacy of velvet antler mushroom polysaccharide, with the ABTS free radical scavenging rate being 80.15%, increasing by 4.78% compared with the prior art, and the half inhibition concentration IC 50 The concentration of 0.39 mg / mL is 11.4% lower than that of the existing technology. Shortening the fermentation cycle can also effectively reduce the probability of microbial contamination, reduce the cost of factory production, achieve cost reduction and increase efficiency, and provide technical support for promoting the development of the velvet antler mushroom industry and the development of new velvet antler mushroom products. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0020] Figure 1 The mycelial growth of Pleurotus eryngii solid culture under different concentrations of Astragalus membranaceus water extract;

[0021] Figure 2 The results of the analysis of the effects of different concentrations of Astragalus membranaceus water extract on the mycelial growth of Pleurotus eryngii solid culture, *: p<0.05, **: p<0.01;

[0022] Figure 3 The mycelial growth of Pleurotus eryngii in liquid culture under different concentrations of Astragalus membranaceus water extract;

[0023] Figure 4 Results of the analysis of the effects of different concentrations of Astragalus membranaceus water extract on the mycelial growth of Pleurotus eryngii in liquid culture, **: p<0.01;

[0024] Figure 5 The mycelial mass of Pleurotus eryngii at different culture times under the action of 1000 mg / L Astragalus membranaceus water extract, **: p<0.01;

[0025] Figure 6 is the glucose standard curve;

[0026] Figure 7 ABTS + The trend of free radical scavenging rate with concentration;

[0027] Figure 8 1g / mL ABTS of Pleurotus eryngii crude polysaccharide + Free radical scavenging rate; *: p<0.05. DETAILED DESCRIPTION

[0028] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0029] It should be understood that the terms described in the present invention are only for describing a particular embodiment and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0030] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0031] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0032] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0033] Example 1 Effect of Astragalus membranaceus Water Extract on Growth and Development of Pleurotus eryngii Mycelium (Solid Culture)

[0034] 1. Prepare a solid culture medium containing the water extract of Astragalus: add the water extract of Astragalus to the liquid basal culture medium (containing agarose) in proportion so that the final concentrations of the water extract of Astragalus are 0, 1 mg / L, 10 mg / L, 100 mg / L, 1000 mg / L, and 10000 mg / L, respectively.

[0035] The preparation of the astragalus water extract is as follows: astragalus powder and water are mixed in a ratio of 100 g: 1000 mL, decocted twice, the decoctions are combined, the decoctions are rotary evaporated at 45° C., and freeze-dried to obtain the astragalus water extract.

[0036] Preparation of liquid basal culture medium: Weigh 200g of potatoes, cut into 2×2cm small pieces, add 800mL of distilled water, heat to boiling, continue to cook on low heat for 25min, filter with gauze, add other components of PDA improved culture medium (8g of peptone, 20g of glucose, 1g of KH2PO4, 0.5g of MgSO4, 110mg of VB), stir and mix well, and make up to 1000mL to prepare liquid basal culture medium.

[0037] Weigh 0.6 g of Astragalus membranaceus water extract into 60 mL of liquid basal culture medium to prepare a 10000 mg / L Astragalus membranaceus water extract mother solution. Prepare 50 mL of solid culture medium according to Table 1, sterilize by high pressure at 121°C, pipette 15 mL into a culture dish while hot, spread it flat and let it stand to cool.

[0038] Table 1 Solid culture medium configuration table

[0039]

[0040] 2. Inoculation. Place the liquid culture of Pleurotus eryngii (with a stirrer in the conical flask) on a magnetic stirrer and culture at 100r / min and 25℃ for 12h to activate the liquid culture. Pipette 0.5mL of liquid culture into the center of the PDA plate without Astragalus membranaceus water extract and culture it at 25℃. After the mycelium grows to cover the plate, use a hole puncher to punch holes at the edge of the mycelium, take the punched mycelium and inoculate it into the center of each treatment group plate, and seal it with sealing film.

[0041] 3. Cultivate. Cultivate at 25℃ for 14 days and observe the mycelial growth every day.

[0042] 4. Determine the growth rate. Determine the mycelium growth diameter and calculate the average mycelium growth rate (mm / d).

[0043] The above experiment was repeated 3 times and the average value was taken. Figure 1 and Figure 2 As shown, the results showed that compared with other treatment groups, the treatment group adding 1000 mg / L Astragalus water extract could significantly promote the growth of Pleurotus eryngii mycelium, and 10000 mg / L Astragalus water extract could significantly inhibit the growth of Pleurotus eryngii mycelium.

[0044] Experimental conclusion: It is preliminarily determined that adding 1000 mg / L Astragalus membranaceus water extract can promote the growth of Pleurotus eryngii mycelium.

[0045] Example 2 Effect of Astragalus membranaceus Water Extract on Growth and Development of Pleurotus eryngii Mycelium (Liquid Culture)

[0046] 1. Prepare a liquid culture medium containing the water extract of Astragalus: add the water extract of Astragalus to the liquid basal culture medium in proportion so that the final concentrations of the water extract of Astragalus are 0, 1 mg / L, 10 mg / L, 100 mg / L, 1000 mg / L, and 10000 mg / L, respectively.

[0047] The preparation of the astragalus water extract is as follows: astragalus powder and water are mixed in a ratio of 100 g: 1000 mL, decocted twice, the decoctions are combined, the decoctions are rotary evaporated at 45° C., and freeze-dried to obtain the astragalus water extract.

[0048] Preparation of liquid basal culture medium: Weigh 200g of potatoes, cut into 2×2cm pieces, add 800mL of distilled water, heat to boiling, continue to cook on low heat for 25min, filter with gauze, add other components of PDA improved culture medium (8g of peptone, 20g of glucose, 1g of KH2PO4, 0.5g of MgSO4, 110mg of VB), stir and mix well, and make up to 1000mL to prepare liquid basal culture medium.

[0049] Weigh 1.2 g of Astragalus membranaceus water extract into 120 mL of liquid basal culture medium to prepare a 10000 mg / L Astragalus membranaceus water extract mother solution. Prepare 100 mL of liquid culture medium according to Table 2, place in a conical flask, autoclave at 121°C, and cool.

[0050] Table 2 Liquid culture medium configuration table

[0051]

[0052] 2. Inoculation. Place the liquid strain of Pleurotus eryngii (with a stirring bar in the conical flask) on a magnetic stirrer and incubate at 100r / min and 25℃ for 12h to activate the liquid strain. Pipette 10mL of the liquid strain into the conical flask and seal it with a breathable membrane.

[0053] 3. Cultivate. Cultivate at 25℃ for 48 hours, and then culture in a shaker at 120r / min and 25℃ until the 7th day.

[0054] 4. Filter and weigh. Pour the mycelium of Pleurotus eryngii onto a Buchner funnel covered with fast filter paper, filter by vacuum, freeze-dry the mycelium in a freeze dryer, collect the mycelium and weigh it.

[0055] The above experiment was repeated 3 times and the average value was taken. Figure 3 and Figure 4 As shown in the results, compared with other groups, the addition of 100-1000 mg / L Astragalus water extract significantly promoted the mycelial growth of Pleurotus eryngii, while 10000 mg / L Astragalus water extract significantly inhibited the mycelial growth of Pleurotus eryngii, which was consistent with the results of solid culture.

[0056] Experimental conclusion: Shan Cancan et al. found that the biomass of Pleurotus eryngii reached a maximum value of 32.81±1.10 g / L on the 8th day (Shan Cancan, Ye Dianzhang, Guo Yu, et al. Optimization of Pleurotus eryngii liquid spawn fermentation formula by response surface methodology [J]. Journal of Fujian Agricultural Sciences, 2023, 38(6): 723-731). In the present invention, after adding 1000 mg / L of Astragalus membranaceus water extract, the biomass of Pleurotus eryngii mycelium reached a maximum value of 35.51 g / L on the 7th day, which increased the yield by 8.23% compared with the prior art. It shows that: (1) Low concentration of Astragalus membranaceus water extract can promote the growth of Pleurotus eryngii mycelium, while high concentration of Astragalus membranaceus water extract inhibits its growth; (2) The optimal concentration of Astragalus membranaceus water extract for the growth of Pleurotus eryngii mycelium is 1000 mg / L.

[0057] Example 3 Effect of adding 1000 mg / L Astragalus membranaceus aqueous extract on the growth and development of Pleurotus eryngii mycelium (liquid culture)

[0058] 1. Prepare a liquid culture medium containing the water extract of Astragalus membranaceus: Add the water extract of Astragalus membranaceus to the liquid basal culture medium in proportion to a final concentration of 1000 mg / L, sterilize at 121°C, cool to below 26°C, and inoculate. The preparation method of the water extract of Astragalus membranaceus and the liquid culture medium is the same as in Example 2.

[0059] 2. Inoculation. Place the liquid strain of Pleurotus eryngii (with a stirring bar in the conical flask) on a magnetic stirrer and incubate at 100r / min and 25℃ for 12h to activate the liquid strain. Pipette 10mL of the liquid strain into the conical flask and seal it with a breathable membrane.

[0060] 3. Cultivation: After static cultivation at 25°C for 48 hours, the culture was placed in a shaker at 120 r / min and 25°C for 11 days, and samples were taken on the 3rd, 5th, 7th, 9th, and 11th days.

[0061] 4. Filter and weigh. Pour the mycelium sample of Pleurotus eryngii onto a Buchner funnel covered with fast filter paper, vacuum filter, freeze-dry the mycelium in a freeze dryer, collect the mycelium and weigh it.

[0062] The above experiment was repeated 3 times and the average value was taken. Figure 5 As shown in the figure, the quality of Pleurotus eryngii mycelium showed a trend of first increasing, entering a plateau period, and then decreasing with the culture time. Pleurotus eryngii mycelium entered a stable period after 7 days of culture. There was no significant difference between the two groups at 7 days and 9 days, and it decreased significantly at 11 days. Therefore, 7 days of culture was selected as the optimal culture time.

[0063] Experimental conclusion: Shan Cancan et al. found that the maximum amount of mycelium of Pleurotus eryngii was on the 8th day (Shan Cancan, Ye Dianzhang, Guo Yu, et al. Optimization of Pleurotus eryngii liquid spawn fermentation formula by response surface methodology [J]. Journal of Fujian Agricultural Sciences, 2023, 38(6): 723-731). The present invention shows that adding 1000 mg / L of Astragalus membranaceus aqueous extract can shorten the liquid fermentation cycle of Pleurotus eryngii by 1 day, which is 12.5% ​​shorter than the prior art.

[0064] Example 4 Extraction of Pleurotus eryngii polysaccharides

[0065] 1. Sample preparation: Take out the mycelium of Pleurotus eryngii and the liquid culture medium in the conical flask of Example 3 and place them in a wide-mouthed stainless steel beaker.

[0066] 2. High-speed shearing: The blade of the high-speed homogenizer is fixed at 2 / 3 below the sample liquid surface, and the suspension is prepared by high-speed shearing at 10,000 r / min for 3 minutes. After centrifugation, the supernatant is collected to obtain a crude polysaccharide solution.

[0067] 3. Determine the concentration of crude polysaccharides using the phenol-sulfuric acid method. Accurately weigh 0.0500g of glucose into a 500mL volumetric flask to make up to volume, and prepare a 0.1g / L glucose standard solution. After diluting with distilled water, take 1mL and add 1mL of 6% phenol solution, add 5.0mL of concentrated sulfuric acid and let stand for 10min. After fully mixing, place in a 30℃ water bath for 20min. Measure the absorbance at a wavelength of 490nm. Draw a standard curve and repeat the above steps to determine the concentration of the crude polysaccharide solution.

[0068] The above experiment was repeated 3 times and the average value was taken. Figure 6 As shown, the glucose standard curve R 2 =0.997, and the concentration of glucose solution and absorbance value have a good linear relationship in the range of 0-0.2 mg / mL, indicating that the obtained formula (Y=0.071+3.233X) can be used for subsequent experiments. The crude polysaccharide content of Porcupine prepared by adding 1000 mg / L Astragalus membranaceus water extract was 9.21 g / L.

[0069] Experimental conclusion: The research of Gao Huijuan et al. showed that the crude polysaccharide content of Pleurotus eryngii fermentation broth was 0.760 g / 100 mL after adding 2 μg / mL Na2SeO3 (Gao Huijuan, Liu Zhifang, Xi Yali, et al. Effect of selenium on extracellular crude polysaccharides of Pleurotus eryngii mycelium and optimization of fermentation conditions [J]. Natural Product Research and Development, 2016, 28(12): 1965-1970). The crude polysaccharide content of Pleurotus eryngii in the present invention was 9.21 g / L, which increased the polysaccharide yield by 21% compared with the process of the prior art.

[0070] Example 5 Determination of the Antioxidant Activity (ABTS Radical Scavenging Ability) of Extracted Crude Polysaccharide Mycelium of Pleurotus eryngii

[0071] 1. Sample preparation. The crude polysaccharide of Pleurotus eryngii prepared in Example 4 was diluted to concentrations of 0.01 mg / mL, 0.05 mg / mL, 0.1 mg / mL, 0.5 mg / mL and 1 mg / mL, respectively. Vitamin C solution (Vc) was used as a positive control.

[0072] 2. Preparation of ABTS working solution: Mix ABTS solution (7 mmol / L, 5 mL) and potassium persulfate solution (140 mmol / L, 88 μL) and react at room temperature in the dark for 12 to 16 hours.

[0073] 3. Adjust the absorbance: Use phosphate buffer (10 mmol / L, pH 7.4) to dilute the working solution until its absorbance at 731 nm is 0.70 ± 0.05.

[0074] 4. Determine the sample absorbance. Take 100 μL of the polysaccharide sample aqueous solution to be tested, add it to 3 mL of ABTS solution, react in the dark for 6 minutes, and record its absorbance at a wavelength of 734 nm as A sample. Replace the polysaccharide sample aqueous solution to be tested with 100 μL of distilled water and perform the above operation, and record its absorbance at a wavelength of 734 nm as A0.

[0075] 5. ABTS + Free radical scavenging ability assay

[0076] ABTS + Free radical scavenging rate = (A0-Asample) / A0×100%.

[0077] Wherein: A0 is the absorbance of the mixed solution with ABTS solution and distilled water; Asample is the absorbance of the mixed solution with ABTS solution and the sample to be tested.

[0078] The above experiment was repeated 3 times and the average value was taken. Figure 7 and Figure 8 As shown in the figure, when the concentration of crude polysaccharide of Pleurotus eryngii increased from 0.01 mg / mL to 0.1 mg / mL, ABTS + The free radical scavenging rate increased slowly; after the concentration reached 0.1 mg / mL, ABTS + The free radical scavenging rate showed a rapid growth trend. When the concentration reached 1 mg / mL, the ABTS of the crude polysaccharide of Pleurotus eryngii added with Astragalus membranaceus water extract was + The free radical scavenging rate was 80.15%. ABTS polysaccharide extracted from antler mushroom after adding Astragalus membranaceus water extract + The free radical scavenging rate was significantly higher than that of the group without adding Astragalus water extract, and there was a significant difference between the two groups.

[0079] Experimental conclusion: Li Wenjiao et al.'s research shows that when the concentration of pilose antler polysaccharide is 1.0 mg / mL, ABTS +The free radical scavenging rate was 76.49% and the half inhibitory concentration IC 50 is 0.44 mg / mL (Li Wenjiao, Wen Shiyong, Zhang Hongyong, et al. Research progress of Pleurotus eryngii [J]. Chinese Edible Fungi, 2022, 41(03): 1-5). In the present invention, the polysaccharide ABTS of Pleurotus eryngii extracted by adding Astragalus membranaceus water extract + The free radical scavenging rate was 80.15%, and the half inhibitory concentration IC 50 It is 0.39 mg / mL, which is 4.78% higher and 11.4% lower than the existing technology.

[0080] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A liquid fermentation medium for Pleurotus eryngii, characterized in that: The composition comprises the following concentrations: 200 g / L potato, 8 g / L peptone, 20 g / L glucose, 1 g / L KH2PO4, 0.5 g / L MgSO4, 10 mg / L VB1, and 100-1000 mg / L astragalus water extract.

2. Use of the Pleurotus eryngii liquid fermentation medium according to claim 1 in promoting Pleurotus eryngii liquid fermentation.

3. The use according to claim 2, characterized in that: The promoting of liquid fermentation of Pleurotus eryngii includes increasing the mycelium yield in the liquid fermentation of Pleurotus eryngii, increasing the polysaccharide yield in the liquid fermentation of Pleurotus eryngii, increasing the polysaccharide efficacy in the liquid fermentation of Pleurotus eryngii and / or shortening the liquid fermentation time of Pleurotus eryngii.

4. A method for increasing mycelium yield in liquid fermentation of Pleurotus eryngii, characterized in that: The method comprises the steps of inoculating the liquid spawn of Pleurotus eryngii into the liquid fermentation medium of Pleurotus eryngii according to claim 1 for fermentation and culturing, so as to increase the mycelium yield in the liquid fermentation of Pleurotus eryngii.

5. A method for increasing the polysaccharide yield in liquid fermentation of Pleurotus eryngii, characterized in that: The method comprises the steps of inoculating the liquid strain of Pleurotus eryngii into the liquid fermentation medium of Pleurotus eryngii according to claim 1 for fermentation culture to increase the polysaccharide yield in the liquid fermentation of Pleurotus eryngii.

6. A liquid fermentation method for improving the efficacy of velvet antler mushroom polysaccharide, characterized in that: The method comprises the steps of inoculating the liquid strain of Pleurotus eryngii into the liquid fermentation medium of Pleurotus eryngii according to claim 1 for fermentation and culture, so as to improve the efficacy of Pleurotus eryngii polysaccharide.

7. A method for shortening the liquid fermentation time of Porcupine antler, characterized in that: The method comprises the steps of inoculating the liquid spawn of Pleurotus eryngii into the liquid fermentation medium of Pleurotus eryngii according to claim 1 for fermentation and culturing, so as to shorten the liquid fermentation time of Pleurotus eryngii.

8. The method according to any one of claims 4 to 7, characterized in that: The fermentation culture temperature is 25° C., the rotation speed is 120 r / min, and the time is 7-11 days.

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