Bactericidal composition for preventing and treating asparagus stem blight and asparagus brown spot

Abstract

The invention relates to the technical field of pesticide chemical industry, and discloses a bactericidal composition for preventing and treating asparagus stem blight and asparagus brown spot, which is prepared from the following raw materials in parts by weight: 20 to 25 parts of paenibacillus polymyxa, 20 to 25 parts of bacillus thuringiensis, 12 to 18 parts of trichoderma, 20 to 25 parts of sophocarpidine, 20 to 25 parts of allicin, 22 to 24 parts of Bordeaux mixture, 15 to 20 parts of dispersing agent, 15 to 20 parts of disintegrating agent, 12 to 18 parts of emulsifying agent, 12 to 18 parts of defoaming agent and 15 to 20 parts of anti-freezing agent. The dispersing agent is lignosulfonate, and the disintegrating agent is sodium carboxymethyl starch. The biological source paenibacillus polymyxa, bacillus thuringiensis and trichoderma, and the plant extract matrine and allicin are used as main components, so that the use of chemical pesticides is reduced, the land pollution and agricultural product pesticide residues are reduced, the ecological balance and food safety are powerfully protected, and greener and healthier asparagus is provided.

Description

Technical Field

[0001] The invention relates to the technical field of pesticide chemicals, in particular to a bactericidal composition for preventing and treating asparagus stem blight and asparagus brown spot. Background Art

[0002] Asparagus, also known as "Reed shoots" and "Nan Reed shoots", is the young shoots of the Liliaceae plant Asparagus reed. It looks similar to the young shoots of the Poaceae plant Phragmites australis, but is completely different. It enjoys the reputation of "King of Vegetables" in the international market. Asparagus is rich in various amino acids, proteins and vitamins, and its content is higher than that of ordinary fruits and vegetables. It has a strong inhibitory effect and pharmacological effect in the prevention and treatment of hypertension, heart disease, leukemia, blood cancer, edema, cystitis, etc. In the past decade, the cultivated area of ​​asparagus has developed rapidly in my country, the export situation is very good, and the economic benefits are very considerable.

[0003] Asparagus stem blight and asparagus brown spot are common diseases in the asparagus cultivation process, which seriously affect the yield and quality of asparagus. At present, the prevention and control of these two diseases mainly rely on chemical fungicides. This prevention and control method will bring about environmental pollution, pesticide residues in agricultural products, destruction of ecological balance and food safety hazards. However, long-term single use can easily lead to drug resistance of pathogens, and the prevention and control effect is poor. Summary of the invention

[0004] In view of the deficiencies of the prior art, the present invention provides a fungicide composition for preventing and treating asparagus stem blight and asparagus brown spot, which solves the problems of land pollution caused by pesticide residues, drug resistance of pathogens and reduced prevention and control effects.

[0005] To achieve the above objectives, the present invention is implemented through the following technical scheme: a bactericidal composition for preventing and treating asparagus stem blight and asparagus brown spot disease, comprising the following raw materials in parts by weight: 20-25 parts of Paenibacillus polymyxa, 20-25 parts of Bacillus thuringiensis, 12-18 parts of Trichoderma, 20-25 parts of matrine, 20-25 parts of allicin, 22-24 parts of Bordeaux mixture, 15-20 parts of dispersant, 15-20 parts of disintegrant, 12-18 parts of emulsifier, 12-18 parts of defoamer and 15-20 parts of antifreeze.

[0006] Preferably, the dispersant is lignin sulfonate, the disintegrant is sodium carboxymethyl starch, the emulsifier is alkylbenzene sulfonate, the defoamer is silicone oil, and the antifreeze agent is ethylene glycol.

[0007] Preferably, the bactericidal composition comprises the following preparation steps:

[0008] Step 1: First, fully mix the prepared active bacterial liquid with the synergistic mixture;

[0009] Step 2: Then, add the filtered and sterilized matrine and allicin, stir at 15-25° C. and 80-150 rpm for 20-30 minutes to fully mix;

[0010] Step 3: finally obtaining the bactericidal composition for preventing and treating asparagus stem blight and asparagus brown spot.

[0011] Preferably, the Bordeaux mixture is prepared by using 20-25 parts of copper sulfate, 20-25 parts of lime and 20-25 parts of water, and the Bordeaux mixture preparation method comprises the following steps:

[0012] (1) First, dissolve copper sulfate in water to prepare a copper sulfate solution;

[0013] (2) dissolving lime in water to make lime milk;

[0014] (3) Then pour the copper sulfate solution into the lime milk and stir continuously to obtain Bordeaux mixture.

[0015] Preferably, the synergistic mixture comprises the following preparation steps:

[0016] The mixed auxiliary agent solution and Bordeaux mixture are placed in a stirring device at 25-40° C. and stirred at 90-200 rpm for 30-60 minutes to obtain a synergistic mixture.

[0017] Preferably, the mixed auxiliary agent solution is prepared by adding a dispersant, a disintegrant, an emulsifier, a defoamer and an antifreeze agent into a stirring device, maintaining 15 to 30° C. and stirring at 50 to 120 rpm for 15 to 40 minutes to obtain a mixed auxiliary agent solution.

[0018] Preferably, in step 1, the method for preparing the active bacterial liquid comprises the following steps:

[0019] Firstly, Paenibacillus polymyxa, Bacillus thuringiensis and Trichoderma are activated and cultured, and the strains of Paenibacillus polymyxa, Bacillus thuringiensis and Trichoderma are inoculated on corresponding culture media respectively, and cultured for 24 to 48 hours at 25 to 30° C. and 150 to 200 r / min to obtain active bacterial liquid.

[0020] Preferably, the filtration sterilization uses a filter membrane with a pore size of 0.22 μm to filter matrine and allicin.

[0021] The present invention provides a fungicide composition for preventing and treating asparagus stem blight and asparagus brown spot disease. It has the following beneficial effects:

[0022] 1. The present invention adopts biological sources of Bacillus polymyxa, Bacillus thuringiensis and Trichoderma, and plant extracts of matrine and allicin as main ingredients, thereby reducing the use of chemical pesticides, reducing land pollution and pesticide residues in agricultural products, effectively protecting ecological balance and food safety, and providing greener and healthier asparagus.

[0023] 2. The present invention cultivates strains targeting asparagus stem blight and asparagus brown spot, and uses matrine and allicin as plant extracts. These targeted ingredient combinations make it difficult for the pathogens to develop drug resistance to all ingredients at the same time. At the same time, the biological strains and plant extracts are more conducive to the absorption of asparagus, reducing the risk of drug resistance.

[0024] 3. The present invention specifically adds Bordeaux mixture, and uses Bordeaux mixture and mixed auxiliary agent solution to prepare a synergistic mixture, which expands the efficacy of the remaining ingredients. After the composition is used, a protective film is formed on the surface of asparagus, ensuring the quality of the sterilization composition and the stability of the effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 The present invention is a schematic diagram of the combined flow of a fungicide composition for preventing and treating asparagus stem blight and asparagus brown spot. DETAILED DESCRIPTION

[0026] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] Please see attached Figure 1 The embodiment of the present invention provides a bactericidal composition for preventing and treating asparagus stem blight and asparagus brown spot, comprising the following raw materials in parts by weight: 20-25 parts of Paenibacillus polymyxa, 20-25 parts of Bacillus thuringiensis, 12-18 parts of Trichoderma, 20-25 parts of matrine, 20-25 parts of allicin, 22-24 parts of Bordeaux mixture, 15-20 parts of dispersant, 15-20 parts of disintegrant, 12-18 parts of emulsifier, 12-18 parts of defoaming agent and 15-20 parts of antifreeze.

[0028] Bacillus polymyxa: can produce antibacterial substances, inhibit the growth of pathogens, promote plant growth and enhance plant immunity.

[0029] Bacillus thuringiensis: It is toxic to many pests and can reduce the damage caused by pests to asparagus, indirectly reducing the occurrence of diseases.

[0030] Trichoderma: It has parasitic and antagonistic effects, can inhibit the growth and reproduction of a variety of plant pathogens, improve the plant rhizosphere microecological environment, and enhance the plant's disease resistance.

[0031] Matrine: A plant-derived alkaloid that has insecticidal and bactericidal effects, has inhibitory effects on a variety of pathogens, and is relatively environmentally friendly and safe.

[0032] Allicin: It has a broad-spectrum antibacterial effect, can inhibit the growth of bacteria and fungi, and enhance the plant's own defense mechanism.

[0033] Bordeaux mixture: can form a protective film on the surface of plants to prevent the invasion of pathogens.

[0034] Lignin sulfonate: helps to evenly disperse various ingredients in the preparation, improving the stability and effectiveness of the preparation.

[0035] Sodium carboxymethyl starch: can promote the rapid disintegration and dispersion of medicines during use and improve the efficacy of the medicines.

[0036] Alkylbenzene sulfonates: Allow oil-soluble and water-soluble ingredients to mix better and form stable emulsions.

[0037] Silicone oil: eliminates foam generated during mixing and production to ensure smooth production and product quality.

[0038] Ethylene glycol: Prevents the product from freezing in low temperature environments and maintains its physical properties and efficacy.

[0039] By using biological agents such as Bacillus polymyxa, Bacillus thuringiensis and Trichoderma, and plant extracts such as matrine and allicin as main ingredients, the use of chemical pesticides can be reduced and the pollution to the land can be reduced.

[0040] The dispersant is lignin sulfonate, the disintegrant is sodium carboxymethyl starch, the emulsifier is alkylbenzene sulfonate, the defoamer is silicone oil, and the antifreeze agent is ethylene glycol.

[0041] The bactericidal composition comprises the following preparation steps:

[0042] Step 1: First, fully mix the prepared active bacterial liquid with the synergistic mixture;

[0043] Step 2: Then, add the filtered and sterilized matrine and allicin, stir at 15-25° C. and 80-150 rpm for 20-30 minutes to fully mix;

[0044] Step 3: finally preparing a fungicide composition for preventing and treating asparagus stem blight and asparagus brown spot.

[0045] The Bordeaux mixture is prepared from 20 to 25 parts of copper sulfate, 20 to 25 parts of lime and 20 to 25 parts of water. The preparation method of the Bordeaux mixture comprises the following steps:

[0046] (1) First, dissolve copper sulfate in water to prepare a copper sulfate solution;

[0047] (2) dissolving lime in water to make lime milk;

[0048] (3) Then pour the copper sulfate solution into the lime milk and stir continuously to obtain Bordeaux mixture.

[0049] The synergistic mixture comprises the following preparation steps:

[0050] The mixed auxiliary agent solution and Bordeaux mixture are placed in a stirring device at 25-40° C. and stirred at 90-200 rpm for 30-60 minutes to obtain a synergistic mixture.

[0051] The mixed auxiliary agent solution is prepared by adding a dispersant, a disintegrant, an emulsifier, a defoamer and an antifreeze agent into a stirring device, maintaining 15 to 30° C. and stirring at 50 to 120 rpm for 15 to 40 minutes to obtain a mixed auxiliary agent solution.

[0052] In step 1, the method for preparing the active bacterial liquid includes the following steps:

[0053] Firstly, Paenibacillus polymyxa, Bacillus thuringiensis and Trichoderma are activated and cultured, and the strains of Paenibacillus polymyxa, Bacillus thuringiensis and Trichoderma are inoculated on corresponding culture media respectively, and cultured for 24 to 48 hours at 25 to 30° C. and 150 to 200 r / min to obtain active bacterial liquid.

[0054] Filtration sterilization Matrine and allicin were filtered using a filter membrane with a pore size of 0.22 μm.

[0055] The following is an introduction in conjunction with specific embodiments:

[0056] Embodiment 1:

[0057] Prepare the following raw materials in parts by weight: 20 parts of Bacillus polymyxa, 20 parts of Bacillus thuringiensis, 12 parts of Trichoderma, 20 parts of matrine, 20 parts of allicin, 22 parts of Bordeaux mixture, 15 parts of lignin sulfonate, 15 parts of sodium carboxymethyl starch, 12 parts of alkylbenzene sulfonate, 12 parts of silicone oil and 15 parts of ethylene glycol.

[0058] Preparation of Bordeaux mixture: Use 20 parts of copper sulfate, 20 parts of lime and 20 parts of water. First, dissolve copper sulfate in water to make copper sulfate solution; then dissolve lime in water to make lime milk; then pour the copper sulfate solution into the lime milk and stir continuously to make Bordeaux mixture.

[0059] Preparation of auxiliary agent solution: add lignin sulfonate, sodium carboxymethyl starch, alkylbenzene sulfonate, silicone oil and ethylene glycol into a stirring device, maintain 15°C and stir at 50 rpm for 15 minutes to obtain a mixed auxiliary agent solution, then put the mixed auxiliary agent solution and Bordeaux mixture into a stirring device at 25°C and stir at 90 rpm for 30 minutes to obtain a synergistic mixture.

[0060] Preparation of bacterial solution: Bacillus polymyxa, Bacillus thuringiensis and Trichoderma were activated and cultured, and their strains were inoculated on corresponding culture media respectively, and cultured at 25°C and 150r / min for 24 hours to obtain active bacterial solution.

[0061] Filtration sterilization: Matrine and allicin were sterilized by filtration using a filter membrane with a pore size of 0.22 μm.

[0062] Preparation of the bactericidal composition: First, the prepared active bacterial liquid and the synergistic mixture are fully mixed; then, matrine and allicin that have been filtered and sterilized are added, and stirred at 15°C and 80 rpm for 20 minutes to fully mix them, and finally a bactericidal composition for preventing and treating asparagus stem blight and asparagus brown spot is prepared.

[0063] Embodiment 2:

[0064] Prepare the following raw materials in parts by weight: 22 parts of Bacillus polymyxa, 22 parts of Bacillus thuringiensis, 15 parts of Trichoderma, 22 parts of matrine, 22 parts of allicin, 23 parts of Bordeaux mixture, 17 parts of lignin sulfonate, 17 parts of sodium carboxymethyl starch, 15 parts of alkylbenzene sulfonate, 15 parts of silicone oil and 17 parts of ethylene glycol.

[0065] Preparation of Bordeaux mixture: Use 22 parts of copper sulfate, 22 parts of lime and 22 parts of water. First, dissolve copper sulfate in water to make copper sulfate solution; then dissolve lime in water to make lime milk; then pour the copper sulfate solution into the lime milk and stir continuously to make Bordeaux mixture.

[0066] Preparation of auxiliary agent solution: lignin sulfonate, sodium carboxymethyl starch, alkylbenzene sulfonate, silicone oil and ethylene glycol are added to a stirring device, maintained at 20°C and mixed and stirred at 80 rpm for 25 minutes to obtain a mixed auxiliary agent solution, and then the mixed auxiliary agent solution and Bordeaux mixture are placed in a stirring device at 30°C and 150 rpm for 45 minutes to obtain a synergistic mixture.

[0067] Preparation of bacterial solution: Bacillus polymyxa, Bacillus thuringiensis and Trichoderma were activated and cultured, and their strains were inoculated on corresponding culture media respectively, and cultured at 28°C and 180r / min for 36 hours to obtain active bacterial solution.

[0068] Filtration sterilization: Matrine and allicin were sterilized by filtration using a filter membrane with a pore size of 0.22 μm.

[0069] Preparation of the bactericidal composition: First, the prepared active bacterial liquid and the synergistic mixture are fully mixed; then, matrine and allicin that have been filtered and sterilized are added, and stirred at 20°C and 120 rpm for 25 minutes to fully mix them, and finally a bactericidal composition for preventing and controlling asparagus stem blight and asparagus brown spot is prepared.

[0070] Embodiment three:

[0071] Prepare the following raw materials in parts by weight: 25 parts of Bacillus polymyxa, 25 parts of Bacillus thuringiensis, 18 parts of Trichoderma, 25 parts of matrine, 25 parts of allicin, 24 parts of Bordeaux mixture, 20 parts of lignin sulfonate, 20 parts of sodium carboxymethyl starch, 18 parts of alkylbenzene sulfonate, 18 parts of silicone oil and 20 parts of ethylene glycol.

[0072] Preparation of Bordeaux mixture: Use 25 parts of copper sulfate, 25 parts of lime and 25 parts of water. First, dissolve copper sulfate in water to make copper sulfate solution; then dissolve lime in water to make lime milk; then pour the copper sulfate solution into the lime milk and stir continuously to make Bordeaux mixture.

[0073] Preparation of auxiliary agent solution: lignin sulfonate, sodium carboxymethyl starch, alkylbenzene sulfonate, silicone oil and ethylene glycol are added to a stirring device, maintained at 30°C and stirred at 120 rpm for 40 minutes to obtain a mixed auxiliary agent solution, and then the mixed auxiliary agent solution and Bordeaux mixture are placed in a stirring device at 40°C and 200 rpm for 60 minutes to obtain a synergistic mixture.

[0074] Preparation of bacterial solution: Bacillus polymyxa, Bacillus thuringiensis and Trichoderma were activated and cultured, and their strains were inoculated on corresponding culture media respectively, and cultured at 30°C and 200r / min for 48 hours to obtain active bacterial solution.

[0075] Filtration sterilization: Matrine and allicin were sterilized by filtration using a filter membrane with a pore size of 0.22 μm.

[0076] Preparation of the bactericidal composition: First, the prepared active bacterial liquid and the synergistic mixture are fully mixed; then, matrine and allicin that have been filtered and sterilized are added, and stirred at 25°C and 150 rpm for 30 minutes to fully mix them, and finally a bactericidal composition for preventing and treating asparagus stem blight and asparagus brown spot is prepared.

[0077] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A fungicidal composition for preventing and treating asparagus stem blight and asparagus brown spot, characterized in that: The invention comprises the following raw materials in parts by weight: 20-25 parts of Paenibacillus polymyxa, 20-25 parts of Bacillus thuringiensis, 12-18 parts of Trichoderma, 20-25 parts of matrine, 20-25 parts of allicin, 22-24 parts of Bordeaux mixture, 15-20 parts of dispersant, 15-20 parts of disintegrant, 12-18 parts of emulsifier, 12-18 parts of defoamer and 15-20 parts of antifreeze.

2. A fungicidal composition for preventing and treating asparagus stem blight and asparagus brown spot according to claim 1, characterized in that: The dispersant is lignin sulfonate, the disintegrant is sodium carboxymethyl starch, the emulsifier is alkylbenzene sulfonate, the defoamer is silicone oil, and the antifreeze is ethylene glycol.

3. A fungicidal composition for preventing and treating asparagus stem blight and asparagus brown spot according to claim 1, characterized in that: The bactericidal composition comprises the following preparation steps: Step 1: First, fully mix the prepared active bacterial liquid with the synergistic mixture; Step 2: Then, add the filtered and sterilized matrine and allicin, stir at 15-25° C. and 80-150 rpm for 20-30 minutes to fully mix; Step 3: finally obtaining the bactericidal composition for preventing and treating asparagus stem blight and asparagus brown spot.

4. A fungicidal composition for preventing and treating asparagus stem blight and asparagus brown spot according to claim 1, characterized in that: The Bordeaux mixture is prepared from 20 to 25 parts of copper sulfate, 20 to 25 parts of lime and 20 to 25 parts of water. The Bordeaux mixture preparation method comprises the following steps: (1) First, dissolve copper sulfate in water to prepare a copper sulfate solution; (2) dissolving lime in water to make lime milk; (3) Then pour the copper sulfate solution into the lime milk and stir continuously to obtain Bordeaux mixture.

5. A fungicidal composition for preventing and treating asparagus stem blight and asparagus brown spot according to claim 3, characterized in that: The synergistic mixture comprises the following preparation steps: The mixed auxiliary agent solution and Bordeaux mixture are placed in a stirring device at 25-40° C. and stirred at 90-200 rpm for 30-60 minutes to obtain a synergistic mixture.

6. A fungicidal composition for preventing and treating asparagus stem blight and asparagus brown spot according to claim 5, characterized in that: The mixed auxiliary agent solution is prepared by adding a dispersant, a disintegrant, an emulsifier, a defoamer and an antifreeze agent into a stirring device, maintaining 15 to 30° C. and stirring at 50 to 120 rpm for 15 to 40 minutes to obtain a mixed auxiliary agent solution.

7. A fungicidal composition for preventing and treating asparagus stem blight and asparagus brown spot according to claim 3, characterized in that: In the step 1, the method for preparing the active bacterial liquid comprises the following steps: Firstly, Paenibacillus polymyxa, Bacillus thuringiensis and Trichoderma are activated and cultured, and the strains of Paenibacillus polymyxa, Bacillus thuringiensis and Trichoderma are inoculated on corresponding culture media respectively, and cultured for 24 to 48 hours at 25 to 30° C. and 150 to 200 r / min to obtain active bacterial liquid.

8. The bactericidal composition for preventing and treating asparagus stem blight and asparagus brown spot according to claim 3, characterized in that: The filtration sterilization uses a filter membrane with a pore size of 0.22 μm to filter matrine and allicin.

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