Chinese herbal medicine antibacterial liquid for inhibiting growth of grass carp pathogenic bacterium aeromonas veronii and preparation method of Chinese herbal medicine antibacterial liquid
The antibacterial solution prepared by using Chinese herbal compositions composed of hematoxylin, pomegranate peel, mugwort leaves, astragalus, coptis chinensis and celery was solved, and the problem of Aeromonas Victoria infection in grass carp farming was achieved, and safe and effective prevention and treatment effects were achieved. .
Patent Information
- Application Number
- CN202411610325.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-12
- Publication Date
- 2025-02-11
AI Technical Summary
In grass carp farming, Aeromonas Vickers infection leads to skin ulcers, bleeding and enteritis. The existing antibiotic prevention and treatment methods pollute the water environment and are prone to drug resistance, making it difficult to effectively prevent and control it.
A Chinese herbal composition composed of hematoxylin, pomegranate peel, mugwort leaves, astragalus, coptis chinensis and chenillus are prepared by decoction and soaking, which is used to inhibit the growth of Aeromonas Vickers.
This Chinese herbal antibacterial solution can significantly inhibit the growth of Aeromonas Vickers, avoid water environment pollution, and reduce the risk of drug resistance, providing a safe and effective prevention and treatment method.
Smart Images
Figure CN119405721A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the field of aquaculture disease prevention and control, and in particular to a Chinese herbal medicine antibacterial liquid for inhibiting the growth of grass carp pathogenic bacteria Aeromonas veronii and a preparation method thereof. Background Art
[0002] Grass carp (Ctenopharyngodon idella) is an important freshwater aquaculture species in my country. However, in recent years, as the density of grass carp farming has gradually increased, bacterial diseases of grass carp have occurred frequently, seriously affecting the production efficiency and economic benefits of grass carp farming (Huang Xinghai, 2023). Among them, infection with Aeromonas vermiformis can cause skin ulcers, bleeding, enteritis and other diseases in grass carp (Wu Yuru, 2022), causing the mortality rate of grass carp in the breeding pond to reach more than 85%. The prevention and control of this bacterium often relies on the use of antibiotics, which not only pollutes the water environment, but also easily produces drug resistance, bringing greater difficulties to the prevention and control of this bacterium.
[0003] Chinese herbal medicines contain a variety of bioactive substances and have the advantages of low price, few side effects, less drug residues in the body and not easy to develop drug resistance. They have been widely used in the prevention and treatment of bacterial diseases (Wang Yu'e, 2008). A series of antibacterial experiments on Chinese herbal medicines were carried out on the pathogenic bacteria Aeromonas veronii in a grass carp breeding pond, and a Chinese herbal medicine formula that can inhibit the growth of this bacteria was developed, so as to better prevent and control the infection of Aeromonas veronii in grass carp. Summary of the invention
[0004] The present invention aims to provide a Chinese medicine composition for inhibiting the growth of grass carp pathogenic bacteria Aeromonas veronii
[0005] Another object of the present invention is to provide a Chinese herbal medicine antibacterial liquid for inhibiting the growth of grass carp pathogenic bacteria Aeromonas veronii.
[0006] Another object of the present invention is to provide a method for preparing the Chinese herbal medicine antibacterial liquid.
[0007] The purpose of the present invention can be achieved through the following technical solutions:
[0008] A traditional Chinese medicine composition is composed of the following traditional Chinese medicines in parts by weight: 9-21 parts of sappan wood, 9-21 parts of pomegranate peel, 20-30 parts of wormwood, 70-82 parts of astragalus, 125-160 parts of coptis root and 125-160 parts of capillaris.
[0009] As a preferred embodiment of the present invention, the Chinese medicine composition is composed of the following Chinese medicinal materials in parts by weight: 13-16 parts of Sappan wood, 13-16 parts of pomegranate peel, 24-28 parts of Artemisia argyi, 72-75 parts of Astragalus, 145-160 parts of Coptis chinensis, and 145-160 parts of Artemisia capillaris.
[0010] As a preferred embodiment of the present invention, the Chinese medicine composition is composed of the following Chinese medicinal materials in parts by weight: 15 parts of Sappan wood, 15 parts of pomegranate peel, 25 parts of Artemisia argyi, 75 parts of Astragalus, 150 parts of Coptis chinensis, and 150 parts of Capillaris.
[0011] The invention discloses an application of the traditional Chinese medicine composition in preparing a product for inhibiting the growth of Aeromonas veronii.
[0012] As a preferred embodiment of the present invention, the Chinese medicine composition is used in the preparation of a Chinese herbal medicine antibacterial liquid for inhibiting the growth of Aeromonas veronii.
[0013] A Chinese herbal medicine antibacterial liquid for inhibiting the growth of Aeromonas veronii is prepared by mixing the water extract of the Chinese medicine composition of the invention.
[0014] A Chinese herbal medicine antibacterial liquid for inhibiting the growth of grass carp pathogenic bacteria Aeromonas veronii is composed of the following formula: 10-20 mg / mL of sappan wood, 10-20 mg / mL of pomegranate peel, 20-30 mg / mL of wormwood, 70-80 mg / mL of astragalus, 125-150 mg / mL of coptis root, and 125-150 mg / mL of capillaris.
[0015] The Chinese herbal antibacterial liquid for inhibiting the growth of grass carp pathogenic bacteria Aeromonas veronii is preferably composed of the following components: 15 mg / mL of Sappan wood, 15 mg / mL of pomegranate peel, 25 mg / mL of Artemisia argyi, 75 mg / mL of Astragalus, 150 mg / mL of Coptis chinensis, and 150 mg / mL of Capillaris.
[0016] The method for preparing the Chinese herbal medicine antibacterial liquid of the present invention comprises the following steps:
[0017] S1: coarsely pulverize each component of the Chinese medicine composition according to claim 1, and then grind it by an ultrafine grinder, and pass it through a 100-mesh sieve to prepare Chinese medicine powder;
[0018] S2: Take a prescribed amount of Sappan wood, soak it in distilled water for 1-1.5 hours, heat and boil the soaked Chinese herbal powder and the soaking liquid, and simmer for 30-45 minutes on low heat. After the liquid is decocted and thickened, filter the liquid, add distilled water again, and repeat the decoction once. Combine the liquids decocted twice and then decoct and concentrate to obtain the Sappan wood stock solution;
[0019] S3: Take a prescribed amount of pomegranate peel, soak it in distilled water for 1-1.5 hours, heat and boil the soaked Chinese herbal medicine powder and the soaking liquid, and simmer for 30-45 minutes on low heat. After the liquid is decocted and thickened, filter the liquid, add distilled water again, and repeat the decoction once. Combine the liquids decocted twice and then decoct and concentrate to obtain the pomegranate peel stock solution;
[0020] S4: Take a prescribed amount of mugwort leaves, soak them in distilled water for 1-1.5 hours, heat and boil the soaked Chinese herbal powder and the soaking liquid, and simmer for 30-45 minutes on low heat. After the liquid is decocted and thickened, filter the liquid, add distilled water again, and repeat the decocting once. Combine the liquids decocted twice and then decoct and concentrate to obtain the mugwort stock solution;
[0021] S5: Take a prescribed amount of Astragalus, soak it in distilled water for 1-1.5 hours, heat and boil the soaked Chinese herbal powder and the soaking solution, and simmer for 30-45 minutes on low heat. After the decoction is thick, filter the decoction, add distilled water again, and repeat the decoction once. Combine the decoctions after the two decoctions and simmer again to obtain the Astragalus stock solution;
[0022] S6: Take a prescribed amount of Coptis chinensis, soak it in distilled water for 1-1.5 hours, heat and boil the soaked Chinese herbal powder and the soaking liquid, and simmer for 30-45 minutes on low heat. After the liquid is decocted and thickened, filter the liquid, add distilled water again, and repeat the decoction once. Combine the liquids decocted twice and then decoct the concentrated Coptis chinensis stock solution;
[0023] S7: Take a formula amount of Artemisia capillaris, soak it in distilled water for 1-1.5 hours, heat and boil the soaked Chinese herbal powder and the soaking liquid, and simmer for 30-45 minutes on low heat. After the liquid is decocted and thickened, filter the liquid, add distilled water again, and repeat the decoction once. Combine the liquids decocted twice and then decoct and concentrate to obtain the Artemisia capillaris stock solution;
[0024] S8: When in use, take equal volumes of the original Chinese medicine solutions and mix them evenly, and make up the volume to 1L with distilled water to prepare the Chinese herbal antibacterial solution.
[0025] As a preferred embodiment of the present invention, the method for preparing the Chinese herbal medicine antibacterial liquid for inhibiting the growth of grass carp pathogenic bacteria Aeromonas veronii comprises the following steps:
[0026] S1: The components of the Chinese herbal medicine are coarsely ground respectively and then crushed by an ultrafine grinder, and passed through a 100-mesh sieve to prepare Chinese herbal medicine powder.
[0027] S2: Take 45g of Sappan wood and soak it in 500mL of distilled water for 1h. Heat and boil the Chinese herbal powder and the soaking solution together, and simmer on low heat for 30min. After the solution becomes thick, filter the solution, add 500mL of distilled water again, and repeat the decoction once. Combine the solutions after the two decoctions and boil again to concentrate to 300mL, so that the final concentration of the Sappan wood stock solution is 150mg / mL.
[0028] S3: Take 45g of pomegranate peel and soak it in 500mL of distilled water for 1h. Heat and boil the soaked Chinese herbal powder and the soaking solution together, and simmer on low heat for 30min. After the solution becomes thick, filter the solution, add 500mL of distilled water again, and repeat the decoction once. Combine the solutions after the two decoctions and boil again to concentrate to 300mL, so that the final concentration of the pomegranate peel stock solution is 150mg / mL.
[0029] S4: Take 75g of mugwort leaves and soak them in 500mL of distilled water for 1h. Heat and boil the soaked Chinese herbal powder and the soaking solution together, and simmer on low heat for 30min. After the solution becomes thick, filter the solution, add 500mL of distilled water again, and repeat the decoction once. Combine the solutions after the two decoctions and boil again to concentrate to 300mL, so that the final concentration of the mugwort solution is 250mg / mL.
[0030] S5: Take 225g of Astragalus and soak it in 3000mL of distilled water for 1h. Heat and boil the Chinese herbal powder and the soaking solution together, and simmer on low heat for 30min. After the solution becomes thick, filter the solution, add 3000mL of distilled water again, and repeat the decoction once. Combine the solutions after the two decoctions and boil again to concentrate to 300mL, so that the final concentration of the Astragalus stock solution is 750mg / mL.
[0031] S6: Take 450g of Coptis chinensis, soak it in 5000mL of distilled water for 1h, heat and boil the soaked Chinese herbal powder and the soaking solution, simmer on low heat for 30min, filter the solution after it becomes thick, add 5000mL of distilled water again, repeat the decoction once, combine the solution after the two decoctions, and boil again to concentrate to 300mL, so that the final concentration of the Coptis chinensis stock solution is 1.5g / mL.
[0032] S7: Take 450g of Artemisia capillaris and soak it in 5000mL of distilled water for 1h. Heat and boil the soaked Chinese herbal powder and the soaking solution together, and simmer on low heat for 30min. After the solution becomes thick, filter the solution, add 5000mL of distilled water again, and repeat the decoction once. Combine the solutions after the two decoctions and boil again to concentrate to 300mL, so that the final concentration of the Artemisia capillaris stock solution is 1.5g / mL.
[0033] S8: When in use, 100 mL of each Chinese medicine liquid is taken out and 400 mL of distilled water is added and mixed, so as to prepare the Chinese herbal medicine antibacterial liquid for inhibiting the growth of grass carp pathogenic bacteria Aeromonas veronii.
[0034] The Chinese herbal medicine antibacterial liquid of the invention can inhibit the growth of Aeromonas veronii, a pathogenic bacterium of grass carp.
[0035] Beneficial effects:
[0036] The invention uses six Chinese herbal medicines, namely, sappan wood, pomegranate peel, wormwood, astragalus, coptis root and capillaris, as raw materials of the antibacterial liquid. The raw materials are easy to obtain and have low cost. Studies have shown that sappan wood contains functional components such as brazilin, sappan phenol, volatile oil and tannin, which can inhibit the growth of Staphylococcus aureus and streptococcus; pomegranate peel contains components such as tannic acid and tannic acid, which can inhibit bacterial growth; wormwood and capillaris contain flavonoid compounds, which can inhibit the growth of common coccus aureus, Escherichia coli and pneumococcus; astragalus polysaccharide in astragalus can inhibit the growth of Escherichia coli and salmonella; berberine in coptis root can interfere with the transport and metabolic process of substances inside and outside bacteria, and inhibit the growth and reproduction of bacteria; the six Chinese herbal medicines are prepared into the antibacterial liquid according to different concentration ratios, which can effectively exert the antibacterial effect, thereby inhibiting the growth of Aeromonas vernix. BRIEF DESCRIPTION OF THE DRAWINGS
[0037] Figure 1 Inhibition zone test DETAILED DESCRIPTION
[0038] Prepare the stock solutions of each component as follows:
[0039] The components of the Chinese herbal medicine are coarsely ground respectively and then crushed by an ultrafine grinder, and passed through a 100-mesh sieve to prepare Chinese herbal medicine powder.
[0040] Take 45g of Sappan wood and soak it in 500mL of distilled water for 1h. Heat and boil the Chinese herbal powder and the soaking solution together, simmer on low heat for 30min, filter the solution after it becomes thick, add 500mL of distilled water again, repeat the decoction once, combine the solutions after the two decoctions and boil again to concentrate to 300mL, so that the final concentration of the Sappan wood stock solution is 150mg / mL.
[0041] Take 45g of pomegranate peel and soak it in 500mL of distilled water for 1h. Heat and boil the soaked Chinese herbal powder and the soaking solution together, simmer on low heat for 30min, filter the solution after it becomes thick, add 500mL of distilled water again, repeat the decoction once, combine the solutions after the two decoctions above and boil again to concentrate to 300mL, so that the final concentration of the pomegranate peel stock solution is 150mg / mL.
[0042] Take 75g of mugwort leaves and soak them in 500mL of distilled water for 1h. Heat and boil the soaked Chinese herbal powder and the soaking solution together, simmer on low heat for 30min, filter the solution after it becomes thick, add 500mL of distilled water again, repeat the decoction once, combine the solutions after the two decoctions above and boil again to concentrate to 300mL, so that the final concentration of the mugwort stock solution is 250mg / mL.
[0043] Take 225g of Astragalus and soak it in 3000mL of distilled water for 1h. Heat the soaked Chinese herbal powder and the soaking solution to boil, simmer on low heat for 30min, filter the solution after it becomes thick, add 3000mL of distilled water again, repeat the decoction once, combine the solution after the above two decoctions and boil it again to concentrate it to 300mL, so that the final concentration of the Astragalus stock solution is 750mg / mL.
[0044] Take 450g of Coptis chinensis and soak it in 5000mL of distilled water for 1h. Heat the soaked Chinese herbal powder and the soaking solution to boil, and simmer on low heat for 30min. After the solution becomes thick, filter the solution, add 5000mL of distilled water again, and repeat the decoction once. Combine the solutions after the two decoctions and boil again to concentrate to 300mL, so that the final concentration of the Coptis chinensis stock solution is 1.5g / mL.
[0045] Take 450g of Artemisia capillaris and soak it in 5000mL of distilled water for 1h. Heat and boil the soaked Chinese herbal powder and the soaking solution together, simmer on low heat for 30min, filter the solution after it becomes thick, add 5000mL of distilled water again, repeat the decoction once, combine the solution after the two decoctions and boil it again to concentrate it to 300mL, so that the final concentration of the Artemisia capillaris stock solution is 1.5g / mL.
[0046] Example 1
[0047] Take 65 mL of Sappan wood stock solution, 65 mL of pomegranate peel stock solution, 80 mL of Artemisia argyi stock solution, 95 mL of Astragalus stock solution, 85 mL of Coptis chinensis stock solution, and 85 mL of Artemisia capillaris stock solution, and add 525 mL of distilled water to prepare the Chinese herbal antibacterial solution.
[0048] Example 2
[0049] Take 120 mL of Sappan wood stock solution, 120 mL of pomegranate peel stock solution, 90 mL of Artemisia argyi stock solution, 100 mL of Astragalus stock solution, 90 mL of Coptis chinensis stock solution, and 90 mL of Artemisia capillaris stock solution, and add 390 mL of distilled water to prepare the Chinese herbal antibacterial solution.
[0050] Example 3
[0051] Take 135 mL of Sappan wood stock solution, 135 mL of pomegranate peel stock solution, 120 mL of Artemisia argyi stock solution, 107 mL of Astragalus stock solution, 100 mL of Coptis chinensis stock solution, and 100 mL of Artemisia capillaris stock solution, and add 303 mL of distilled water to prepare the Chinese herbal antibacterial solution.
[0052] Example 4
[0053] Take 100mL of Sappan wood stock solution, 100mL of pomegranate peel stock solution, 100mL of Artemisia argyi stock solution, 100mL of Astragalus stock solution, 100mL of Coptis chinensis stock solution, 100mL of Capillaris stock solution, and add 400mL of distilled water to prepare the Chinese herbal antibacterial solution.
[0054] Example 5
[0055] Take 100 mL of Sappan wood stock solution, 65 mL of pomegranate peel stock solution, 90 mL of Artemisia argyi stock solution, 95 mL of Astragalus stock solution, 95 mL of Coptis chinensis stock solution, 100 mL of Artemisia capillaris stock solution, and add 455 mL of distilled water to prepare the Chinese herbal antibacterial solution.
[0056] Example 6
[0057] Take 130 mL of Sappan wood stock solution, 130 mL of pomegranate peel stock solution, 110 mL of Artemisia argyi stock solution, 100 mL of Astragalus stock solution, 100 mL of Coptis chinensis stock solution, 90 mL of Artemisia capillaris stock solution, and add 340 mL of distilled water to prepare the Chinese herbal antibacterial solution.
[0058] Example 7
[0059] Antibacterial experiment: Aeromonas verticillioides was provided by the Fish Disease Department of Jiangsu Freshwater Fisheries Research Institute. Prepare a nutrient agar plate with a thickness of 10-12mm, and use a pipette to draw 150μL of Aeromonas verticillioides bacterial solution and evenly spread it on the nutrient agar plate. Then use a sterile hole puncher with a diameter of 7mm to punch holes on the plate, and punch 4 holes on each plate; among them, 3 holes in plate 1 are added with the Chinese herbal medicine solution of Examples 1-3, and 1 hole is used as a negative control. 3 holes in plate 2 are added with the Chinese herbal medicine solution of Examples 4-6, and 1 hole is used as a negative control, and they are marked. The amount of Chinese herbal medicine solution added to each well is 200μL, and 200μL of sterile distilled water is added to the negative control well. The plate is cultured in a constant temperature incubator at 32℃ for 24h, and the diameter of the antibacterial zone is measured and recorded with a vernier caliper.
[0060] Table 1 Diameter of inhibition zone
[0061] Group Diameter of inhibition zone (mm) Example 1 15.88 Example 2 13.68 Example 3 16.53 Control group 1 0.00 Example 4 17.45 Example 5 13.81 Example 6 14.29 Control group 2 0.00
[0062] Table 1 Combination Figure 1 It shows that: compared with control groups 1 and 2, Examples 1-6 all have antibacterial effects, among which Example 4 has the largest diameter of the antibacterial circle, indicating the strongest antibacterial effect. The results show that the Chinese herbal liquid composition of the present invention can significantly inhibit the growth of Aeromonas vernix, and the concentration ratio of each Chinese herbal liquid in Example 4 is the optimal ratio.
[0063] Example 8
[0064] Add 9 mL of 3.1×10 4 cfu / mL Aeromonas vervetii suspension and 1 mL of the Chinese herbal antibacterial solution of Example 4, add 9 mL of a 3.1×10 4cfu / mL of Aeromonas veronii bacterial suspension and 1 mL of sterile distilled water, and the number of Aeromonas veronii in test tube 1 and test tube 2 was measured at 2h, 8h, 16h, and 32h, respectively.
[0065] Table 2
[0066]
[0067] The results are shown in Table 2: Compared with test tube 2, test tube 1 had sterile growth within 24 hours, and as time went on, bacteria slowly grew after 48 hours. Through comparative experiments, it was concluded that the antibacterial Chinese herbal medicine can also significantly inhibit the growth of grass carp pathogenic bacteria Aeromonas veronii.
Claims
1. A Chinese medicine composition, characterized in that: The invention is composed of the following Chinese medicinal materials in parts by weight: 9-21 parts of Sappan wood, 9-21 parts of pomegranate peel, 20-30 parts of Artemisia argyi, 70-82 parts of Astragalus, 125-160 parts of Coptis chinensis and 125-160 parts of Artemisia capillaris.
2. The Chinese medicine composition according to claim 1, characterized in that: The invention is composed of the following Chinese medicinal materials in parts by weight: 13-16 parts of Sappan wood, 13-16 parts of pomegranate peels, 24-28 parts of Artemisia argyi, 72-75 parts of Astragalus, 145-160 parts of Coptis chinensis and 145-160 parts of Artemisia capillaris.
3. The Chinese medicine composition according to claim 2, characterized in that: The invention is composed of the following Chinese medicinal materials in parts by weight: 15 parts of Sappan wood, 15 parts of pomegranate peels, 25 parts of Artemisia argyi, 75 parts of Astragalus, 150 parts of Coptis chinensis and 150 parts of Capillaris wormwood.
4. Use of the Chinese medicine composition according to any one of claims 1 to 3 in the preparation of a product for inhibiting the growth of Aeromonas veronii.
5. The use according to claim 4, characterized in that: Use of the Chinese medicine composition according to any one of claims 1 to 3 in the preparation of a Chinese herbal medicine antibacterial liquid for inhibiting the growth of Aeromonas veronii.
6. A Chinese herbal medicine antibacterial liquid for inhibiting the growth of Aeromonas veronii, characterized in that: The invention is prepared by mixing the aqueous extracts of the Chinese medicine composition according to any one of claims 1 to 3.
7. The Chinese herbal antibacterial liquid according to claim 6, characterized in that: It is composed of the following components: 9-21 mg / mL of Sappan wood, 9-21 mg / mL of pomegranate peel, 20-30 mg / mL of Artemisia argyi, 70-82 mg / mL of Astragalus, 125-160 mg / mL of Coptis chinensis, and 125-160 mg / mL of Artemisia capillaris.
8. The Chinese herbal antibacterial liquid according to claim 7, characterized in that: It is composed of the following components: 15 mg / mL of Sappan wood, 15 mg / mL of pomegranate peel, 25 mg / mL of Artemisia argyi, 75 mg / mL of Astragalus, 150 mg / mL of Coptis chinensis, and 150 mg / mL of Capillaris.
9. The method for preparing the Chinese herbal medicine antibacterial solution according to claim 6, characterized in that: The following steps are involved: S1: coarsely pulverize each component of the Chinese medicine composition according to claim 1, and then grind it by an ultrafine grinder, and pass it through a 100-mesh sieve to prepare Chinese medicine powder; S2: Take a prescribed amount of Sappan wood, soak it in distilled water for 1-1.5 hours, heat and boil the soaked Chinese herbal powder and the soaking liquid, and simmer for 30-45 minutes on low heat. After the liquid is decocted and thickened, filter the liquid, add distilled water again, and repeat the decoction once. Combine the liquids decocted twice and then decoct and concentrate to obtain the Sappan wood stock solution; S3: Take a prescribed amount of pomegranate peel, soak it in distilled water for 1-1.5 hours, heat and boil the soaked Chinese herbal medicine powder and the soaking liquid, and simmer for 30-45 minutes on low heat. After the liquid is decocted and thickened, filter the liquid, add distilled water again, and repeat the decoction once. Combine the liquids decocted twice and then decoct and concentrate to obtain the pomegranate peel stock solution; S4: Take a prescribed amount of mugwort leaves, soak them in distilled water for 1-1.5 hours, heat and boil the soaked Chinese herbal powder and the soaking liquid, and simmer for 30-45 minutes on low heat. After the liquid is decocted and thickened, filter the liquid, add distilled water again, and repeat the decocting once. Combine the liquids decocted twice and then decoct and concentrate to obtain the mugwort stock solution; S5: Take a prescribed amount of Astragalus, soak it in distilled water for 1-1.5 hours, heat and boil the soaked Chinese herbal powder and the soaking solution, and simmer for 30-45 minutes on low heat. After the decoction is thick, filter the decoction, add distilled water again, and repeat the decoction once. Combine the decoctions after the two decoctions and simmer again to obtain the Astragalus stock solution; S6: Take a prescribed amount of Coptis chinensis, soak it in distilled water for 1-1.5 hours, heat and boil the soaked Chinese herbal powder and the soaking liquid, and simmer for 30-45 minutes on low heat. After the liquid is decocted and thickened, filter the liquid, add distilled water again, and repeat the decoction once. Combine the liquids decocted twice and then decoct the concentrated Coptis chinensis stock solution; S7: Take a formula amount of Artemisia capillaris, soak it in distilled water for 1-1.5 hours, heat and boil the soaked Chinese herbal powder and the soaking liquid, and simmer for 30-45 minutes on low heat. After the liquid is decocted and thickened, filter the liquid, add distilled water again, and repeat the decoction once. Combine the liquids decocted twice and then decoct and concentrate to obtain the Artemisia capillaris stock solution; S8: When in use, take equal volumes of the original Chinese medicine solutions and mix them evenly, and make up the volume to 1 L with distilled water to prepare the Chinese herbal antibacterial solution as claimed in claim 6.
10. The preparation method according to claim 9, characterized in that: The following steps are involved: S1: coarsely pulverize each component of the Chinese medicine composition according to claim 1, and then grind it by an ultrafine grinder, and pass it through a 100-mesh sieve to prepare Chinese medicine powder; S2: Take 45g of Sappan wood and soak it in 500mL of distilled water for 1h. Heat and boil the Chinese herbal powder and the soaking solution together, and simmer on low heat for 30min. After the solution becomes thick, filter the solution, add 500mL of distilled water again, and repeat the decoction once. Combine the solutions after the two decoctions and boil again to concentrate to 300mL, so that the final concentration of the Sappan wood stock solution is 150mg / mL. S3: Take 45g of pomegranate peel, soak it in 500mL of distilled water for 1h, heat and boil the soaked Chinese herbal medicine powder and the soaking solution, simmer for 30min on low heat, filter the solution after it becomes thick, add 500mL of distilled water again, repeat the decocting once, combine the decocted solutions twice, and boil and concentrate to 300mL, so that the final concentration of the pomegranate peel stock solution is 150mg / mL; S4: Take 75g of mugwort leaves, soak them in 500mL of distilled water for 1h, heat and boil the soaked Chinese herbal powder and the soaking solution, simmer for 30min on low heat, filter the solution after it thickens, add 500mL of distilled water again, repeat the decocting once, combine the decocted solutions twice, and then boil and concentrate to 300mL, so that the final concentration of the mugwort leaf stock solution is 250mg / mL; S5: Take 225g of Astragalus, soak it in 3000mL of distilled water for 1h, heat the soaked Chinese herbal powder and the soaking solution to boil, simmer for 30min on low heat, filter the solution after it thickens, add 3000mL of distilled water again, repeat the decoction once, combine the decoctions after the two decoctions, and decoct and concentrate to 300mL, so that the final concentration of the Astragalus stock solution is 750mg / mL; S6: Take 450g of Coptis chinensis, soak it in 5000mL of distilled water for 1h, heat and boil the soaked Chinese herbal powder and the soaking solution, and simmer for 30min on low heat. After the solution is thickened, filter the solution, add 5000mL of distilled water again, and repeat the simmering once. Combine the solutions after the two simmerings and simmer again to concentrate to 300mL, so that the final concentration of the Coptis chinensis stock solution is 1.5g / mL; S7: Take 450g of Artemisia capillaris, soak it in 5000mL of distilled water for 1h, heat and boil the Chinese herbal powder and the soaking solution, simmer for 30min on low heat, filter the solution after the solution thickens, add 5000mL of distilled water again, repeat the decoction once, combine the solution after the above two decoctions and boil it again to concentrate to 300mL, so that the final concentration of the Artemisia capillaris stock solution is 1.5g / mL; S8: When in use, 100 mL of each Chinese medicine liquid is taken out and 400 mL of distilled water is added and mixed, so as to prepare the Chinese herbal medicine antibacterial liquid as claimed in claim 6.