Kit for osteoclast identification

Abstract

The invention provides a kit for osteoclast identification. The kit comprises a box body of a hollow structure, a rotating shaft rotationally connected to the middle of the inner side of the box body, and a plurality of tube storage mechanisms arranged around the rotating shaft; the tube storage mechanism comprises a connecting block connected with the rotating shaft, a guide frame arranged on the connecting block, and a material placing tube which is connected to the guide frame in a sliding manner and is used for placing test tubes. By rotating the rotating shaft, the material placing pipe drives a test tube to rotate, so that a reagent and a sample in the test tube are shaken up, and when the material placing pipe rotates, the covering assembly blocks the test tube, so that the test tube can be prevented from sliding upwards in the material placing pipe and falling off from the material placing pipe; meanwhile, when the material placing pipe rotates along the rotating shaft, the contact rod is extruded through the extrusion block, so that the material placing pipe can do reciprocating motion in the radial direction of the rotating shaft, and samples and reagents in the test tubes are further uniformly shaken.

Description

Technical Field

[0001] The present invention relates to the field of biomedical technology, and in particular to a kit for identifying osteoclasts. Background Art

[0002] Osteoclasts are usually identified and analyzed using the TRAP staining method, and reagent tubes and kits are often used for identification and analysis of osteoclasts, that is, the reagents and samples are placed in the reagent tubes, and then the reagent tubes are placed in the kits for full reaction before identification and analysis;

[0003] The patent with publication number: CN221139064U discloses an osteoclast identification kit which is easy to keep warm and shake, including a cover plate, an outer box body and an inner box body, the cover plate is installed on the outer box body, a bearing is arranged at the middle position of the cover plate, a rotating sleeve is passed through the bearing, a hand crank is arranged on the rotating sleeve, and the inner box body is driven to rotate by the hand crank, so as to facilitate the shaking of the reagents in the test tubes in the kit; however, in the above patent, the test tubes are easy to move up and down when shaking, thereby causing damage to the test tubes, and the effect of shaking the reagents is low. Summary of the invention

[0004] Based on this, the object of the present invention is to provide a test tube for osteoclast identification, which is not easy to move when the test tube is shaken and has high reagent shaking efficiency.

[0005] The invention provides the following technical solutions: a kit for identifying osteoclasts, comprising a box body with a hollow structure, a rotating shaft rotatably connected to the middle part of the inner side of the box body, and a plurality of tube storage mechanisms arranged around the rotating shaft; the tube storage mechanism comprises a connecting block connected to the rotating shaft, a guide frame arranged on the connecting block, a feeding tube for placing test tubes slidably connected to the guide frame, a plurality of first springs arranged between the feeding tube and the guide frame, and a covering mechanism for preventing the test tube from moving upward is arranged on the upper part of the feeding tube; the kit also comprises a pushing mechanism for driving the feeding tube to move radially along the rotating shaft; the rotation of the rotating shaft drives the tube storage mechanism to rotate, thereby causing the tube storage mechanism to drive the test tube to rotate, and while the test tube follows the rotation of the tube storage mechanism, the pushing mechanism causes the test tube to reciprocate radially along the rotating shaft.

[0006] Furthermore, the covering mechanism includes a first bracket arranged on the feeding tube, a flip cover rotatably connected to the first bracket, and a plurality of torsion springs arranged between the flip cover and the first bracket, and the flip cover is located on the upper side of the feeding tube.

[0007] Furthermore, the pushing mechanism includes a contact rod arranged on the material placing tube of each of the storage tube mechanisms, and a plurality of extrusion blocks arranged around the inner wall of the box body, and when the contact rod rotates along the axis of the rotating shaft, the contact rod and the extrusion block are extruded and matched.

[0008] Furthermore, a push-and-turn mechanism is slidably connected to the rotating shaft for pushing all the flip covers to rotate and open. The push-and-turn mechanism includes a sliding plate slidably connected to the rotating shaft, a contact block provided on each of the flip covers, and a driving component for driving the sliding plate to move axially toward the rotating shaft, and the sliding plate moves to squeeze the contact block.

[0009] Furthermore, first sliding grooves in a straight line shape are provided on both axial sides of the rotating shaft, and the sliding plate is slidably connected to the rotating shaft via the first sliding grooves.

[0010] Furthermore, the driving assembly includes a first rotating seat arranged on the rotating shaft, a threaded rod rotatably connected to the first rotating seat, and a threaded sleeve arranged on the sliding disk, and the threaded rod is threadedly connected to the threaded sleeve.

[0011] Furthermore, a rotating cover is rotatably connected to the box body, and the rotating cover is used to seal the interior of the box body. A rotating mechanism is connected between the rotating cover and the rotating shaft to shake the rotating shaft for rotation.

[0012] Furthermore, the rotating mechanism includes a second rotating seat rotatably connected to the rotating cover, a sliding rod slidably connected to the second rotating seat, a second protrusion arranged on the end of the sliding rod close to the rotating shaft, a first protrusion arranged on the upper part of the rotating shaft, and a crank handle arranged on the end of the sliding rod away from the rotating shaft, and the first protrusion and the second protrusion are interfered with each other.

[0013] Furthermore, the sliding rod is provided with a first stopper and a second stopper, there is a gap between the first stopper and the second stopper, the second rotating seat is located between the first stopper and the second stopper, a first groove is opened at the upper end of the rotating shaft, and the first protrusion is arranged on the side wall of the first groove.

[0014] Furthermore, the test kit also includes a locking mechanism for limiting the rotation of the rotating cover, the locking mechanism includes a second slide groove opened on the rotating cover, a slider slidably connected in the second slide groove, a second spring arranged between the slider and the rotating cover, a pushing block arranged on the slider, a second bracket arranged on the box body, a limit block arranged on the second bracket, a second groove opened on the limit block, and inclined surfaces arranged on both sides of the limit block, and the rotating cover cannot rotate when the slider is located in the second groove.

[0015] The beneficial effects of the invention are as follows: by rotating the rotating shaft, the rotating shaft drives the connecting block to rotate, the connecting block drives the feeding tube to rotate, and the feeding tube drives the test tube to rotate, so that the reagent and sample in the test tube are shaken evenly, and the covering component blocks the test tube when the feeding tube rotates, thereby preventing the test tube from sliding upward in the feeding tube and preventing the test tube from falling out of the feeding tube, and at the same time, when the feeding tube rotates along the rotating shaft, the feeding tube also drives the contact rod to rotate, and when the contact rod rotates and contacts the extrusion block, the extrusion block squeezes the contact rod, and the feeding tube moves in the direction close to the rotating shaft, and the first spring is compressed. When the contact rod is separated from the extrusion block, the first spring resets and drives the feeding tube to reset, thereby causing the feeding tube to reciprocate radially along the rotating shaft, so that the sample and reagent in the test tube are further shaken evenly. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention.

[0017] Figure 2 It is a schematic diagram of the three-dimensional structure inside the box body of the present invention.

[0018] Figure 3 It is a three-dimensional structural schematic diagram of the pipe storage mechanism and the pushing mechanism of the present invention.

[0019] Figure 4 It is a schematic diagram of the three-dimensional structure of the covering assembly of the present invention.

[0020] Figure 5 It is a three-dimensional structural schematic diagram of the push-and-turn mechanism of the present invention.

[0021] Figure 6 It is a schematic diagram of the three-dimensional structure of the rotating mechanism of the present invention.

[0022] Figure 7 It is a three-dimensional structural schematic diagram of the locking mechanism of the present invention.

[0023] The markings in the accompanying drawings are: 1-box body, 2-rotating shaft, 3-storage tube mechanism, 31-connecting block, 32-guide frame, 33-feeding tube, 34-first spring, 35-covering assembly, 351-first bracket, 352-flip cover, 353-torsion spring, 4-test tube, 5-push-turn mechanism, 51-first slide groove, 52-sliding plate, 53-first rotating seat, 54-threaded rod, 55-threaded sleeve, 56-contact block, 6-pushing mechanism, 61-contact rod, 62-extrusion block, 7-rotating cover, 8-rotating mechanism, 81-first groove, 82-first protrusion, 83-second rotating seat, 84-sliding rod, 85-first block, 86-crank handle, 87-second block, 88-second protrusion, 9-locking mechanism, 91-second slide groove, 92-second spring, 93-sliding block, 94-pushing block, 95-second bracket, 96-limiting block, 97-second groove, 98-inclined surface. DETAILED DESCRIPTION

[0024] In order to facilitate the understanding of the present invention, the present invention will be described more fully below with reference to the relevant drawings. Several embodiments of the present invention are given in the drawings. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present invention more thorough and comprehensive.

[0025] It should be noted that when an element is referred to as being "fixed to" another element, it may be directly on the other element or there may be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only.

[0026] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0027] A kit for identifying osteoclasts, such as Figure 1-Figure 5As shown, it is a first embodiment of the present invention, comprising a box body 1 with a hollow structure, a rotating shaft 2 rotatably connected to the middle of the inner side of the box body 1, and a plurality of tube storage mechanisms 3 arranged around the rotating shaft 2; in this embodiment, there are five tube storage mechanisms 3, which can be increased or decreased appropriately in other embodiments, and the tube storage mechanism 3 comprises a connecting block 31 connected to the rotating shaft 2, a guide frame 32 arranged on the connecting block 31, a placing tube 33 for placing a test tube 4 slidably connected to the guide frame 32, and a plurality of first placing tubes 33 and the guide frame 32 arranged between the placing tube 33 and the guide frame 32. Spring 34, in this embodiment, two first springs 34 are provided on both sides of the guide frame 32, and a covering mechanism for preventing the test tube 4 from moving upward is provided on the upper part of the feeding tube 33; the reagent kit also includes a pushing mechanism 6 for driving the feeding tube 33 to move radially along the rotating shaft 2; the rotation of the rotating shaft 2 drives the tube storage mechanism 3 to rotate, so that the tube storage mechanism 3 drives the test tube 4 to rotate, and when the test tube 4 rotates following the tube storage mechanism 3, the test tube 4 is moved back and forth radially along the rotating shaft 2 through the pushing mechanism 6.

[0028] It can be understood that the operator first opens the covering mechanism, and then the operator can place the test tube 4 added with reagents and samples into the feeding tube 33, and then close the covering mechanism to place the test tube 4 firmly in the feeding tube 33, and then rotate the rotating shaft 2, the rotating shaft 2 drives the connecting block 31 to rotate, the connecting block 31 drives the feeding tube 33 to rotate, and the feeding tube 33 drives the test tube 4 to rotate, so that the reagents and samples in the test tube 4 are evenly shaken, and when the feeding tube 33 rotates, the covering assembly 35 blocks the test tube 4, thereby preventing the test tube 4 from sliding upward in the feeding tube 33 and preventing the test tube 4 from falling out of the feeding tube 33. At the same time, when the feeding tube 33 rotates along the rotating shaft 2, the pushing mechanism 6 can move the feeding tube 33 radially along the rotating shaft 2, thereby further making the test tube 4 evenly mixed.

[0029] The covering mechanism includes a first bracket 351 arranged on the feeding tube 33, a flip cover 352 rotatably connected to the first bracket 351, and a plurality of torsion springs 353 arranged between the flip cover 352 and the first bracket 351. The flip cover 352 is located on the upper side of the feeding tube 33.

[0030] It can be understood that when the operator needs to place the test tube 4 into the feeding tube 33, the operator can rotate the flip cover 352 upwards, the torsion spring 353 is deformed, and then the test tube 4 is placed in the feeding tube 33, and then the flip cover 352 is released, the torsion spring 353 is reset, and the torsion spring 353 drives the flip cover 352 to reset so that the flip cover 352 rests on the upper side of the test tube 4, thereby firmly placing the test tube 4 in the feeding tube 33, which can effectively prevent the test tube 4 from falling off from the feeding tube 33 during rotation.

[0031] The pushing mechanism 6 includes a contact rod 61 arranged on the material placing tube 33 of each of the tube storage mechanisms 3 , and a plurality of extrusion blocks 62 arranged around the inner wall of the box body 1 . When the contact rod 61 rotates along the axis of the rotating shaft 2 , the contact rod 61 is extruded and matched with the extrusion blocks 62 .

[0032] It can be understood that when the feeding tube 33 rotates along the axis of the rotating shaft 2, the feeding tube 33 will drive the contact rod 61 to rotate. When the contact rod 61 rotates and contacts the extrusion block 62, the extrusion block 62 squeezes the contact rod 61, and the feeding tube 33 moves toward the rotating shaft 2. The first spring 34 is compressed. When the contact rod 61 is separated from the extrusion block 62, the first spring 34 is reset to drive the feeding tube 33 to reset, thereby making the feeding tube 33 reciprocate along the radial direction of the rotating shaft 2, so that the test tube 4 in the feeding tube 33 is shaken evenly.

[0033] A push-and-turn mechanism 5 for pushing all the flip covers 352 to rotate and open is slidably connected to the rotating shaft 2. The push-and-turn mechanism 5 includes a sliding plate 52 slidably connected to the rotating shaft 2, a contact block 56 arranged on each of the flip covers 352, and a driving component for driving the sliding plate 52 to move axially toward the rotating shaft 2. The sliding plate 52 moves to squeeze the contact block 56. A first straight-shaped slide groove 51 is provided on both axial sides of the rotating shaft 2. The sliding plate 52 is slidably connected to the rotating shaft 2 through the first slide groove 51. The driving component includes a first rotating seat 53 arranged on the rotating shaft 2, a threaded rod 54 rotatably connected to the first rotating seat 53, and a threaded sleeve 55 arranged on the sliding plate 52. The threaded rod 54 is threadedly connected to the threaded sleeve 55.

[0034] It can be understood that when the operator needs to fully open the flip cover 352, the operator can rotate the threaded rod 54 forward, the threaded rod 54 drives the threaded sleeve 55 to move downward, the threaded sleeve 55 moves downward and drives the sliding plate 52 to move downward, the sliding plate 52 moves downward to squeeze the contact block 56, and the contact block 56 drives the flip cover 352 to rotate upward and open. At this time, the operator can place the test tube 4 into the placing tube 33. After placing the test tube 4, the operator can rotate the threaded rod 54 in the opposite direction, the threaded rod 54 drives the threaded sleeve 55 to move upward, the threaded sleeve 55 drives the sliding plate 52 to move upward, the sliding plate 52 no longer squeezes the contact block 56, and the torsion spring 353 drives the flip cover 352 to rest against the upper end of the test tube 4.

[0035] like Figure 6 As shown, it is a second embodiment of the present invention, which, based on the first embodiment, also includes: a rotating cover 7 is rotatably connected to the box body 1, and the rotating cover 7 is used to achieve the internal closure of the box body 1, and a rotating mechanism 8 for shaking the rotating shaft 2 is connected between the rotating cover 7 and the rotating shaft 2, and the rotating mechanism 8 includes a second rotating seat 83 rotatably connected to the rotating cover 7, a sliding rod 84 slidably connected to the second rotating seat 83, a second protrusion 88 arranged on the end of the sliding rod 84 close to the rotating shaft 2, a first protrusion 82 arranged on the upper part of the rotating shaft 2, and a crank handle 86 arranged on the end of the sliding rod 84 away from the rotating shaft 2, the first protrusion 82 and the second protrusion 88 are interference-fitted, the upper end of the rotating shaft 2 is provided with a groove, and the first protrusion 82 is arranged on the side wall of the groove.

[0036] It can be understood that after the operator has placed all the test tubes 4 into the placing tube 33, the operator can rotate the rotating cover 7 to rotate the rotating cover 7 to the opening of the box body 1, so that the inside of the box body 1 is sealed to prevent the internal components of the box body 1 from contacting the outside and improve safety. After the rotating cover 7 has sealed the box body 1, the operator can slide the slide bar 84 downward, and the slide bar 84 drives the second protrusion 88 to slide downward, so that the second protrusion 88 slides into the first groove 81, and then the operator can shake the handle 86, and the handle 86 drives the slide bar 84 to rotate, and the slide bar 84 drives the second rotating seat 83 to rotate on the rotating cover 7, and the slide bar 84 drives the second rotating seat 83 to rotate. The rod 84 drives the second protrusion 88 to rotate, and the rotation of the second protrusion 88 will contact the first protrusion 82, thereby pushing the first protrusion 82 to rotate along the axis of the rotating shaft 2, thereby the first protrusion 82 will drive the rotating shaft 2 to rotate, so that the test tube 4 in the box body 1 is shaken evenly. When the test tube 4 in the box body 1 needs to be taken out, the operator can slide the sliding bar 84 upward, and the sliding bar 84 drives the second protrusion 88 to slide upward, so that the second protrusion 88 is no longer located in the first groove 81, and then the operator rotates the rotating cover 7 so that the rotating cover 7 no longer blocks the opening of the box body 1, and then the test tube 4 in the box body 1 can be taken out.

[0037] The slide bar 84 is provided with a first stopper 85 and a second stopper 87 . There is a gap between the first stopper 85 and the second stopper 87 . The second rotating seat 83 is located between the first stopper 85 and the second stopper 87 .

[0038] It can be understood that the sliding distance of the slide bar 84 can be limited by the action of the first stop block 85 and the second stop block 87. That is to say, when the first stop block 85 contacts the rotating cover 7, the second protrusion 88 is located in the first groove 81, and when the second stop block 87 contacts the rotating cover 7, the second protrusion 88 is no longer located in the first groove 81.

[0039] like Figure 6 As shown, it is the third embodiment of the present invention, and this embodiment further includes, based on the second embodiment: the reagent kit also includes a locking mechanism 9 for limiting the rotation of the rotating cover 7, the locking mechanism 9 includes a second slide groove 91 provided on the rotating cover 7, a slider 93 slidably connected in the second slide groove 91, a second spring 92 arranged between the slider 93 and the rotating cover 7, a pushing block 94 arranged on the slider 93, a second bracket 95 provided on the box body 1, a limiting block 96 provided on the second bracket 95, a second groove 97 provided on the limiting block 96, and inclined surfaces 98 arranged on both sides of the limiting block 96, and the rotating cover 7 cannot rotate when the slider 93 is located in the second groove 97.

[0040] When the rotatable cover 7 is rotated to close the opening of the box body 1, the rotatable cover 7 will drive the slider 93 to rotate. When the slider 93 rotates, it will contact the inclined surface 98 of the limit block 96. The inclined surface 98 will squeeze the slider 93, and the slider 93 will slide to the inner side of the rotatable cover 7. The second spring 92 is compressed. When the slider 93 is disengaged from the inclined surface 98, the slider 93 will align with the second groove 97. At this time, the second spring 92 is reset to drive the slider 93 to move into the second groove 97. The second groove 97 will limit the rotation of the slider 93, thereby limiting the rotation of the rotatable cover 7. When it is necessary to remove the rotatable cover 7 from the opening of the box body 1, the operator can pull the push block 94 to drive the slider 93 to move to the inner side of the rotatable cover 7. The second spring 92 is compressed, and the slider 93 will no longer be located in the second groove 97. Then the operator rotates the rotatable cover 7 so that the rotatable cover 7 is no longer located at the opening of the box body 1.

[0041] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

[0042] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A kit for identifying osteoclasts, characterized in that: The invention comprises a box body with a hollow structure, a rotating shaft rotatably connected to the middle part of the inner side of the box body, and a plurality of tube storage mechanisms arranged around the rotating shaft; the tube storage mechanism comprises a connecting block connected to the rotating shaft, a guide frame arranged on the connecting block, a feeding tube for placing test tubes slidably connected to the guide frame, a plurality of first springs arranged between the feeding tube and the guide frame, and a covering mechanism for preventing the test tube from moving upward is arranged on the upper part of the feeding tube; the test kit also comprises a pushing mechanism for driving the feeding tube to move radially along the rotating shaft; the rotation of the rotating shaft drives the tube storage mechanism to rotate, thereby causing the tube storage mechanism to drive the test tube to rotate, and when the test tube follows the rotation of the tube storage mechanism, the pushing mechanism causes the test tube to reciprocate radially along the rotating shaft.

2. The kit according to claim 1, characterized in that The covering mechanism includes a first bracket arranged on the material placing tube, a flip cover rotatably connected to the first bracket, and a plurality of torsion springs arranged between the flip cover and the first bracket. The flip cover is located on the upper side of the material placing tube.

3. The kit according to claim 1, characterized in that The pushing mechanism includes a contact rod arranged on the material placing tube of each of the tube storage mechanisms, and a plurality of extrusion blocks arranged around the inner wall of the box body. When the contact rod rotates along the axis of the rotating shaft, the contact rod is extruded and matched with the extrusion blocks.

4. The kit according to claim 2, characterized in that A push-and-turn mechanism is slidably connected to the rotating shaft and is used to push all the flip covers to rotate and open. The push-and-turn mechanism includes a sliding plate slidably connected to the rotating shaft, a contact block provided on each flip cover, and a driving component for driving the sliding plate to move axially toward the rotating shaft, and the sliding plate moves to squeeze the contact block.

5. The kit according to claim 4, characterized in that A first sliding groove in the shape of a straight line is provided on both axial sides of the rotating shaft, and the sliding plate is slidably connected to the rotating shaft through the first sliding groove.

6. The kit according to claim 4, characterized in that The driving assembly includes a first rotating seat arranged on the rotating shaft, a threaded rod rotatably connected to the first rotating seat, and a threaded sleeve arranged on the sliding disk, wherein the threaded rod is threadably connected to the threaded sleeve.

7. The kit according to claim 1, characterized in that A rotating cover is rotatably connected to the box body, and the rotating cover is used to seal the interior of the box body. A rotating mechanism is connected between the rotating cover and the rotating shaft to shake the rotating shaft for rotation.

8. The kit according to claim 7, characterized in that The rotating mechanism includes a second rotating seat rotatably connected to the rotating cover, a sliding rod slidably connected to the second rotating seat, a second protrusion arranged on an end of the sliding rod close to the rotating shaft, a first protrusion arranged on the upper part of the rotating shaft, and a crank handle arranged on an end of the sliding rod away from the rotating shaft, the first protrusion and the second protrusion having an interference fit.

9. The kit according to claim 8, characterized in that The slide bar is provided with a first stopper and a second stopper, there is a gap between the first stopper and the second stopper, the second rotating seat is located between the first stopper and the second stopper, a first groove is opened at the upper end of the rotating shaft, and the first protrusion is arranged on the side wall of the first groove.

10. The kit according to claim 7, characterized in that The test kit also includes a locking mechanism for limiting the rotation of the rotating cover, the locking mechanism includes a second sliding groove provided on the rotating cover, a slider slidably connected in the second sliding groove, a second spring arranged between the slider and the rotating cover, a pushing block arranged on the slider, a second bracket provided on the box body, a limiting block provided on the second bracket, a second groove provided on the limiting block, and inclined surfaces provided on both sides of the limiting block, and the rotating cover cannot rotate when the slider is located in the second groove.

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