Application of titanium-doped platanus acerifolia dead leaf carbon quantum dots as plant nano seed soaking agent in improving germination rate of rice seeds under salt stress
通过钛掺杂悬铃木枯叶碳量子点作为植物纳米浸种剂,解决了盐胁迫下水稻种子萌发率低的问题,实现了在盐胁迫条件下的高效萌发率提升,同时保证了材料的环保和安全 sex.
Patent Information
- Application Number
- CN202411886680.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-20
- Publication Date
- 2025-02-14
AI Technical Summary
Under salt stress conditions, the germination rate of rice seeds is low, and affected by ion, osmotic and oxidative stress, existing metal/metal oxide nanoparticles have environmental pollution and biosafety problems.
Titanium-doped cyclist dead leaf carbon quantum dots are used as plant nanoseed impregnation agents, and titanium-doped cyclist dead leaf carbon quantum dots are synthesized through hydrothermal reactions to prepare cheap, safe and efficient seeding liquid to soak rice seeds to improve germination rate.
It effectively improves the germination rate of rice seeds under salt stress, and the materials are environmentally friendly and low in cost, and there are no environmental pollution and biosafety problems.
Smart Images
Figure CN119422515A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of crop planting, and particularly relates to application of titanium-doped carbon quantum dots from dead plane leaves as plant nano seed soaking agents to improve the germination rate of rice seeds under salt stress. Background Art
[0002] In recent years, soil salinization has become increasingly serious due to extreme weather, unreasonable irrigation methods, fertilizer application, and industrial pollution. With the continuous accumulation of soil salt, the degradation of soil texture, and the increase in the content of harmful substances, rice seeds are subjected to ion stress, osmotic stress, and oxidative stress, which restricts the germination of rice. Plant nanobiology is an emerging, cutting-edge, and interdisciplinary research field. It comes from the deep integration of nanotechnology and plant science, and has a unique idea for improving the salt tolerance of crops. Currently, nanomaterials such as iron oxide nanoparticles, zinc oxide nanoparticles, and cerium oxide nanoparticles, which are widely reported, have achieved pro-oxidation / antioxidation balance defense capabilities in a variety of higher plants under abiotic stress conditions. However, pure metal / metal oxide nanoparticles are costly, easily release unstable multivalent heavy metal ions, and have potential environmental pollution and biosafety issues.
[0003] Carbon dots (CDs) are a new type of zero-dimensional carbon-based nanomaterial with a size less than 10nm, with a chemical structure consisting of a central carbon core and surface functional groups (such as hydroxyl and carboxyl groups). They have excellent physical and chemical properties and good antioxidant properties, low cost, biofriendliness and high stability. In addition, titanium can improve the yield and quality of crops, enable crops to better adapt to the complex and changing natural environment, and enhance their stress resistance. At present, there are no reports on the use of garden plant litter to synthesize carbon dots to improve the germination rate of rice seeds under salt stress. Summary of the invention
[0004] In view of this, the purpose of the present invention is to provide an application of titanium-doped sycamore dead leaf carbon quantum dots as a plant nano seed soaking agent to improve the germination rate of rice seeds under salt stress. The titanium-doped sycamore dead leaf carbon quantum dots used in the present invention are pollution-free to the environment, cheap and easy to obtain, and have a simple synthesis method. Soaking rice seeds in a seed soaking solution prepared with titanium-doped sycamore dead leaf carbon quantum dots can effectively improve the germination rate under salt stress.
[0005] In order to achieve the above object, the present invention provides the following technical solutions:
[0006] The invention provides application of titanium-doped dead plane tree leaf carbon quantum dots as plant nano seed soaking agents for improving the germination rate of rice seeds under salt stress.
[0007] Preferably, the preparation method of the titanium-doped sycamore dead leaf carbon quantum dots is:
[0008] collecting dead plane leaves, cleaning them, drying them, and then crushing them to obtain dead plane leaf powder;
[0009] The sycamore dead leaf powder, water and nano-titanium dioxide are mixed and stirred, and then subjected to a hydrothermal reaction. After the hydrothermal reaction is completed, centrifugation is performed to obtain titanium-doped sycamore dead leaf carbon quantum dots.
[0010] Preferably, the drying temperature is 50-60° C., more preferably 55° C., and the drying time is 3-5 h, more preferably 4 h.
[0011] Preferably, the volume mass ratio of the sycamore dead leaf powder, nano titanium dioxide and water is (1-2) g:150 mg:(60-100) mL, and more preferably 1 g:150 mg:60 mL.
[0012] Preferably, the mixing and stirring time is 5 to 15 minutes, more preferably 10 minutes.
[0013] Preferably, the temperature of the hydrothermal reaction is 150-220° C., more preferably 200° C., and the time is 4-8 h, more preferably 6 h.
[0014] Preferably, the centrifugal speed is 6000-10000 rpm, more preferably 8000 rpm, and the time is 20-30 min, more preferably 25 min.
[0015] Preferably, the preparation method of the plant nano seed soaking agent is: mixing titanium-doped sycamore dead leaf carbon quantum dots with water to obtain the plant nano seed soaking agent.
[0016] Preferably, the volume mass ratio of the titanium-doped sycamore dead leaf carbon quantum dots to water is (50-250) μg:1 mL, and more preferably 150 μg:1 mL.
[0017] Preferably, the specific method of the application is: soaking the rice seeds in the seed soaking agent for 12 to 48 hours, more preferably for 24 hours.
[0018] Preferably, the crop seeds are pretreated before soaking, and the pretreatment process is: select crop seeds with full grains, uniform size and no damage, disinfect the surface with 2% to 5% sodium hypochlorite solution for 10 to 15 minutes, then rinse with deionized water for 5 to 10 minutes, and place the disinfected seeds at room temperature to dry for use.
[0019] Contains at least the following beneficial technical effects:
[0020] The present invention dopes titanium dioxide nanoparticles into CDs derived from dead leaves of sycamore, and synthesizes doped dead leaves of sycamore carbon quantum dots (Ti-CDs) by a one-step hydrothermal method, which can be used after simple centrifugal filtration. The dead leaves of sycamore used in the present invention are garden plant waste, cheap and easy to obtain; the synthesis method is simple, and the equipment used is common; the synthesized nano seed soaking agent can be used after simple filtration; soaking rice seeds with Ti-CDs can effectively improve the germination rate under salt stress. BRIEF DESCRIPTION OF THE DRAWINGS
[0021] Figure 1 This is the TEM image of titanium-doped sycamore dead leaf carbon quantum dots prepared in Example 1.
[0022] Figure 2 This is a fluorescence emission spectrum of titanium-doped sycamore dead leaves carbon quantum dots prepared in Example 1; Figure 2 A is the fluorescence emission spectrum of titanium-doped sycamore dead leaves carbon quantum dots at different excitation wavelengths. Figure 2 B is the excitation spectrum diagram of the titanium-doped sycamore dead leaf carbon quantum dots in the present invention at λem=470nm and the emission spectrum diagram at λex=350nm.
[0023] Figure 3 This is the XRD pattern of titanium-doped sycamore dead leaf carbon quantum dots prepared in Example 1.
[0024] Figure 4 This is the XPS image of titanium-doped sycamore dead leaf carbon quantum dots prepared in Example 1; Figure 4 A, 4B, 4C and 4D are C1s, N 1s, O 1s and Ti 2p in titanium-doped sycamore leaf carbon quantum dots, respectively.
[0025] Figure 5 It is a diagram of seed germination in Experimental Example 1; wherein A is a seed that grows normally after being soaked in clean water, marked as CK; B is a seed that grows normally after being soaked in the titanium-doped carbon quantum dots of dead leaves of sycamore prepared in Example 1, marked as Ti-CDs-150; C is a seed that grows under NaCl stress for 7 days after being soaked in clean water, marked as NaCl; D is a seed that grows under NaCl stress for 7 days after being soaked in the titanium-doped carbon quantum dots of dead leaves of sycamore prepared in the present invention, marked as NaCl+Ti-CDs-150.
[0026] Figure 6 This is a graph showing the determination of superoxide dismutase (SOD) and peroxidase (POD) levels in rice seedlings in Experimental Example 1, in which the rice seeds were immersed in titanium-doped carbon quantum dots from dead plane leaves for 24 hours and then grown under NaCl stress for 7 days. DETAILED DESCRIPTION
[0027] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.
[0028] It should be understood that the terms described in the present invention are only for describing special embodiments and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. Each smaller range between the intermediate value in any stated value or stated range and any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.
[0029] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.
[0030] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present application description and examples are exemplary only.
[0031] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.
[0032] The "room temperature" and "normal temperature" mentioned in the present invention are all calculated as 25±2°C unless otherwise specified.
[0033] Unless otherwise specified, the raw materials used in the following examples of the present invention are all commercially available.
[0034] Example 1
[0035] Preparation of Titanium-doped Plantain Tree Dead Leaves Carbon Quantum Dots:
[0036] Collecting and cleaning the dead leaves of sycamore trees, drying them in a drying oven at 55° C. for 4 h, and then crushing them to obtain dead leaves of sycamore trees powder;
[0037] Weigh 1 g of dead leaf powder of sycamore tree, 150 mg of titanium dioxide nanoparticles, and 60 ml of deionized water, mix and stir for 10 minutes, and then put them into a 100 ml reactor for hydrothermal reaction; the hydrothermal reaction temperature is 200° C. and the reaction time is 6 hours.
[0038] After the hydrothermal reaction is completed and cooled, the liquid is taken out and divided into 50 mL centrifuge tubes, centrifuged at 8000 rpm for 25 min, and the supernatant is filtered to obtain a brown-yellow liquid. After freeze-drying, it is finally stored in a -20°C refrigerator to obtain titanium-doped sycamore dead leaf carbon quantum dots.
[0039] Preparation of plant nano seed soaking agent:
[0040] Titanium-doped sycamore dead leaf carbon quantum dots and water were evenly mixed at a ratio of 150 μg:1 mL to obtain a plant nano seed soaking agent (Ti-CDs-150 seed soaking solution).
[0041] The titanium-doped sycamore dead leaf carbon quantum dots obtained in Example 1 were characterized.
[0042] Figure 1 The morphology of the titanium-doped dead plane tree leaf carbon quantum dots of the present invention is as follows: Figure 1 The quasi-spherical carbon quantum dots can be clearly seen, with particle sizes less than 10 nm, an average particle size of 1.69 to 2.36 nm, and a relatively uniform distribution, which is one of the reasons for the good stability of the carbon quantum dots.
[0043] Figure 2 The fluorescence and ultraviolet absorption spectra of the titanium-doped sycamore dead leaf carbon quantum dot solution of the present invention are shown. Figure 2 A shows the excitation wavelength at λ ex = When the excitation wavelength λ is enhanced in the range of 310 to 440 nm, the excitation-dependent emission spectrum of the synthesized carbon quantum dots changes. ex =310~350nm gradually increases, and then as the excitation wavelength changes from λ ex =350~440nm increases and decreases, so the excitation wavelength λ is selected ex =350nm is the best excitation wavelength. This may be due to the different sizes of carbon quantum dots, or different surface states and distribution of functional groups. Figure 2 B once again confirms the location of the maximum excitation wavelength and the maximum emission wavelength.
[0044] Figure 3 is the XRD diagram of the titanium-doped sycamore dead leaf carbon quantum dot powder of the present invention, Figure 3 It can be seen that the titanium-doped sycamore dead leaf carbon quantum dots have an amorphous structure and a broad (002) crystal plane diffraction peak of graphite carbon at around 22.5°.
[0045] Figure 4 This is the XPS graph of the titanium-doped sycamore dead leaf carbon quantum dot powder of the present invention. Figure 4 A shows that there are characteristic peaks at 288.3eV, 286.4eV, and 284.8eV, corresponding to C=O, CO / CN, and CC / C=C, respectively, indicating the presence of C1s in titanium-doped sycamore dead leaf carbon quantum dots. Figure 4 B shows that there are characteristic peaks at 402.2eV, 400.4eV, and 399.5eV, corresponding to NH, N-C3, and CNC, respectively, indicating the presence of N1s in the titanium-doped sycamore dead leaf carbon quantum dots. Figure 4 It can be seen from C that there are characteristic peaks at 533.6eV, 532.7eV, 531.7eV, and 530.6eV, corresponding to C=O, C-OH, CO, and Metal-O, respectively, indicating the presence of O1s in the titanium-doped sycamore dead leaf carbon quantum dots. Figure 4 D shows that there are two characteristic peaks at 466.0eV and 459.7eV, which belong to Ti 2p 1 / 2 and Ti 2p 3 / 2， This indicates the presence of Ti 2p in titanium-doped sycamore leaf carbon quantum dots, confirming the successful doping of titanium.
[0046] Example 2
[0047] Preparation of Titanium-doped Plantain Tree Dead Leaves Carbon Quantum Dots:
[0048] Collecting and cleaning the dead leaves of sycamore trees, drying them in a drying oven at 50° C. for 3 h, and then crushing them to obtain dead leaves of sycamore trees powder;
[0049] 1.5 g of dead leaf powder of sycamore tree, 150 mg of titanium dioxide nanoparticles, and 80 ml of deionized water were weighed, mixed and stirred for 5 minutes, and then placed in a 100 ml reactor for hydrothermal reaction; the hydrothermal reaction temperature was 150° C. and the reaction time was 4 hours.
[0050] After the hydrothermal reaction is completed and cooled, the liquid is taken out and divided into 50 ml centrifuge tubes, centrifuged at 6000 rpm for 20 min, and the supernatant is filtered to obtain a brown-yellow liquid. After freeze-drying, it is finally stored in a -20°C refrigerator to obtain titanium-doped sycamore dead leaf carbon quantum dots.
[0051] Preparation of plant nano seed soaking agent:
[0052] The titanium-doped sycamore dead leaf carbon quantum dots and water were evenly mixed at a ratio of 50 μg:1 mL to obtain a plant nano seed soaking agent (Ti-CDs-50 seed soaking solution).
[0053] Example 3
[0054] Preparation of Titanium-doped Plantain Tree Dead Leaves Carbon Quantum Dots:
[0055] Collecting and cleaning the dead leaves of sycamore trees, drying them in a drying oven at 60° C. for 5 h, and then crushing them to obtain dead leaves of sycamore trees powder;
[0056] Weigh 2 g of dead leaf powder of sycamore tree, 150 mg of titanium dioxide nanoparticles, and 100 ml of deionized water, mix and stir for 15 minutes, and then put them into a 100 ml reactor for hydrothermal reaction; the hydrothermal reaction temperature is 220° C. and the reaction time is 8 hours.
[0057] After the hydrothermal reaction is completed and cooled, the liquid is taken out and divided into 50 ml centrifuge tubes, centrifuged at 10000 rpm for 30 min, the supernatant is filtered to obtain a brown-yellow liquid, which is finally stored in a -20°C refrigerator to obtain titanium-doped sycamore dead leaf carbon quantum dots.
[0058] Preparation of plant nano seed soaking agent:
[0059] Titanium-doped sycamore dead leaf carbon quantum dots and water were evenly mixed at a ratio of 250 μg:1 mL to obtain a plant nano seed soaking agent (Ti-CDs-250 seed soaking solution).
[0060] Experimental Example 1
[0061] Select rice seeds with full grains, uniform size and no damage, disinfect them with 5% sodium hypochlorite solution for 15 minutes, wash them with deionized water for 10 minutes, and place the disinfected seeds at room temperature to dry for later use.
[0062] Then, the seeds were soaked in the soaking solutions obtained in Examples 1-3 for 24 hours, and the seeds were soaked in clear water as a control. After soaking in the same manner, 100 mM NaCl solution was used for salt stress.
[0063] The soaked seeds were placed in a culture dish covered with germination paper, and the culture dish was placed in an incubator at 25 degrees Celsius, with 18 hours of light and 6 hours of darkness. The germination rate, biomass and root length of each group of seeds were recorded, see Table 1, Table 2 and Table 3, respectively.
[0064] After seven days of stress, the plants were taken out, photographed, and their biomass and root length were weighed. Figure 5 , where A is the seeds soaked in water and growing normally, marked as CK; B is the seeds soaked in the titanium-doped sycamore dead leaves carbon quantum dots prepared in Preparation Example 1 and growing normally, marked as Ti-CDs-150; C is the seeds soaked in water and grown under NaCl stress for 7 days, marked as NaCl; D is the seeds soaked in the titanium-doped sycamore dead leaves carbon quantum dots prepared in the present invention and grown under NaCl stress for 7 days, marked as NaCl+Ti-CDs-150. After seven days, compared with CK, the rice seeds soaked with Ti-CDs germinated faster ( Figure 5 ), under NaCl stress, rice seeds soaked with Ti-CDs germinated faster, indicating that Ti-CDs alleviated the salt stress of rice seeds ( Figure 5 ).
[0065] After seven days of stress, the rice seedlings were taken for superoxide dismutase (SOD) and peroxidase (POD) assays. Figure 6 .Depend on Figure 6 A and Figure 6 B shows that exposure to NaCl stress significantly reduced the antioxidant capacity of rice, and the expression level was significantly decreased compared with the CK group. On the contrary, after soaking seeds with Ti-CDs, the antioxidant system was well restored. The above results show that Ti-CDs can effectively alleviate salt stress by improving the antioxidant capacity of rice seedlings.
[0066] Similarly, the germination rate, biomass, and seed root length of Ti-CDs-50 in Example 2 and Ti-CDs-250 in Example 3 were tested and recorded according to the above method, as shown in Tables 1 to 3.
[0067] Table 1 Statistical results of seed germination rate in each group
[0068]
[0069]
[0070] Table 2 Statistical results of seed biomass in each group
[0071]
[0072] Table 3 Statistical results of seed root length in each group
[0073]
[0074] It can be seen from Tables 1 to 3 above that the titanium-doped sycamore dead leaf carbon quantum dots provided by the present invention have a significant effect on improving the germination of rice seeds under salt stress, and have a promoting effect at a concentration of 50 to 250 μg / mL.
[0075] The present invention uses the dead leaves of sycamore to synthesize titanium-doped dead leaves of sycamore carbon quantum dots (Ti-CDs) by one-step hydrothermal method, and can be used after simple treatment. The material of the present invention is dead leaves of sycamore, which are plant litters, which are simple and easy to obtain; the synthesis method is simple, and the equipment used is common; the synthesized nano seed soaking agent can be used after simple centrifugation, filtration, and freeze drying; soaking rice seeds with Ti-CDs can effectively improve the germination rate of rice.
[0076] The titanium-doped dead plane tree leaf carbon quantum dots in the present invention can be used alone or combined with other reagents to prepare a seed soaking agent.
[0077] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.
Claims
1. Application of titanium-doped carbon quantum dots from dead sycamore leaves as plant nanoseed soaking agents to improve the germination rate of rice seeds under salt stress.
2. The use according to claim 1, characterized in that: The preparation method of the titanium-doped sycamore dead leaf carbon quantum dots is as follows: collecting dead plane leaves, cleaning them, drying them, and then crushing them to obtain dead plane leaf powder; The sycamore dead leaf powder, water and nano-titanium dioxide are mixed and stirred, and then subjected to a hydrothermal reaction. After the hydrothermal reaction is completed, centrifugation is performed to obtain titanium-doped sycamore dead leaf carbon quantum dots.
3. The use according to claim 2, characterized in that: The drying temperature is 50-60° C. and the drying time is 3-5 hours.
4. The use according to claim 2, characterized in that: The volume mass ratio of the sycamore dead leaf powder, nano titanium dioxide and water is (1-2) g:150 mg:(60-100) mL.
5. The use according to claim 2, characterized in that: The mixing and stirring time is 5 to 15 minutes.
6. The use according to claim 2, characterized in that: The temperature of the hydrothermal reaction is 150-220° C. and the time is 4-8 hours.
7. The use according to claim 2, characterized in that: The centrifugal speed is 6000-10000 rpm, and the time is 20-30 min.
8. The use according to claim 1, characterized in that: The preparation method of the plant nano seed soaking agent comprises the following steps: mixing titanium-doped sycamore dead leaf carbon quantum dots with water to obtain the plant nano seed soaking agent.
9. The use according to claim 8, characterized in that: The concentration of the plant nano seed soaking agent is 50-250 μg / mL.
10. The use according to claim 1, characterized in that: The specific application method is: soaking rice seeds in the seed soaking agent for 12 to 48 hours.