Seed bud grafting method for Brazil rubber tree with petioles and leaf buds

Abstract

The invention relates to the technical field of rubber tree seedling cultivation, in particular to a Brazil rubber tree seed seedling bud grafting method with petioles and leaf buds, which comprises the following steps: sowing Brazil rubber tree seeds in a sand bed, and cultivating to obtain a seed seedling stock with the seeds; the method comprises the following steps: selecting bud sticks of a rubber tree for scion, taking off bud slices with petioles and leaf buds, and pruning to obtain scion bud slices; selecting a flat surface of a seedling stock, and opening an interface on a base part; the scion bud slices are grafted to the interfaces in a bud grafting mode, and bud-grafted seedlings are obtained; the bud-grafted seedlings are transplanted, and bud-grafted nursery stocks are obtained; after conventional management is conducted on the bud grafting seedlings, petioles are removed, then leaf marks are covered, watering and fertilization are conducted every other day, culture management is conducted, and seed bud grafting seedlings are obtained; the petiole removing comprises the following steps of: ripping a plastic film at the base part of the petiole, taking down the petiole by hand, and covering a leaf mark by using the film. According to the seedling bud grafting method for the Brazil rubber tree with the petioles and the leaf buds, the leaf buds with the petioles can be fully utilized on the premise that the bud grafting speed and the bud grafting survival rate are not reduced.

Description

Technical Field

[0001] The present application relates to the technical field of rubber tree seedling cultivation, and in particular to a method for bud grafting seedlings of Hevea brasiliensis with petiole leaf buds. Background Art

[0002] Hevea brasiliensis, also known as rubber tree, is native to the western part of the Amazon River Basin in Brazil. It is a typical tropical rainforest tree species of the genus Hevea in the Euphorbiaceae family. It is also an important economic crop in my country and tropical regions around the world. The latex it produces is the main raw material for natural rubber. Natural rubber is an important strategic material and industrial raw material. Although there are more than 2,000 rubber-producing plants in the world, about 98% of the natural rubber currently used still comes from rubber trees.

[0003] At present, the main method for obtaining Brazilian rubber tree planting materials is budding. Budding is an asexual reproduction method that uses buds of excellent asexual lines as scions and grafts them onto seedling rootstocks. It can not only maintain the excellent characteristics of the scion variety, but also utilize the favorable characteristics of the rootstock. Rubber budding includes large seedling budding, small seedling budding and seedling budding. According to the type of bud sticks, there are conventional bud sticks, green bud sticks and micro bud sticks. The seedling budding technology was invented by the Rubber Research Institute of the South China Academy of Tropical Crops in 1988. According to the inventor's report, this technology uses seedlings with seeds as rootstocks, with a seedling height of 20 to 30 cm and a diameter of about 0.3 cm; the bud pieces should be green bud sticks (bud stick diameter is 0.5 to 1.2 cm) with scale buds and leaf buds that have lost their petioles. The budding survival rate of scale buds is particularly consistent. When performing the budding operation, the size of the bud interface of the rootstock is 1 / 2 of the diameter, and the width of the bud piece taken is equivalent to or slightly smaller than the bud interface, about 0.30 to 0.35 cm wide and 2 to 3 cm long.

[0004] The Rubber Research Institute of the South China Academy of Tropical Crops also recorded in the seedling budding technology: the budding survival rate of scale buds is 64% to 76%, the budding survival rate of petiole-free leaf buds is 88%, and the budding survival rate of petiole-attached leaf buds is 15% to 36%. The budding survival rate of petiole-attached leaf buds is significantly lower than that of scale buds and petiole-attached leaf buds. With the promotion and development of seedling budding technology, scale buds and petiole-attached leaf buds are still the most commonly used bud types. Petiole-attached leaf buds are usually discarded directly due to the shortcomings of too large buds and small adaptability to rootstocks, and the budded seedlings will die due to the rotting of petioles. They are rarely used in seedling budding, resulting in low utilization of buds in the current seedling budding technology. In the existing technology, there is little content related to seedling budding with petiole-attached leaf buds.

[0005] Therefore, a new seedling budding method is needed to solve the current situation that the bud pieces with petiole leaf buds are difficult to be used in seedling budding technology. Summary of the invention

[0006] The problem that the present application aims to solve is to provide a method for budding seedlings of Hevea brasiliensis with petiole leaf buds, which can make full use of the leaf buds with petioles without reducing the budding speed and the budding survival rate.

[0007] In order to solve the above technical problems, this application adopts the following technical solutions:

[0008] The present application provides a method for budding seedlings of a Brazilian rubber tree with petiole leaf buds, comprising the following steps:

[0009] Seeds of the Brazilian rubber tree are sown in a sand bed to cultivate a seedling stock with seeds; bud strips of the rubber tree for scion are selected, bud pieces with petiole leaf buds are removed, and pruning is performed to obtain scion bud pieces; a flat surface of the seedling stock is selected, and an interface is opened at the base; the scion bud piece is bud-grafted on the interface to obtain a bud-grafted seedling; the bud-grafted seedling is transplanted to obtain a bud-grafted seedling; and the bud-grafted seedling is subjected to conventional management, the petiole is removed, and then the leaf marks are covered, watering and fertilizing are performed every other day, and cultivation management is performed to obtain a seedling bud-grafted seedling;

[0010] Wherein, the step of removing petioles comprises:

[0011] Cut the plastic film at the base of the petiole, remove the petiole by hand, and then cover the leaf scar with the film.

[0012] In some embodiments, the shoots include shoots whose canopy leaves are in a stable stage to a sprouting stage.

[0013] In some embodiments, the method of removing petiolate leaf buds comprises a peeling method.

[0014] In some embodiments, the interface comprises a large bud interface, the width of the large bud interface is 3 / 5 to 4 / 5 of the circumference of the stem of the seedling rootstock, and the length of the large bud interface is 30 to 50 mm.

[0015] In some embodiments, the length of the scion bud is 29.22-42.83 mm, the width of the scion bud is 7.34-10.87 mm, and the petiole length of the scion bud is 4.08 mm-8.85 mm.

[0016] In some embodiments, the incubation period is 3 to 4 weeks.

[0017] In some embodiments, the duration of routine management is 10 to 14 days.

[0018] In some embodiments, the budding method includes: placing the scion bud at the interface, bundling it from bottom to top with a film, and when it reaches the position of the petiole, first wrapping it from top to bottom for 2 to 3 turns, and then continuing to wrap it upward until the interface is completely covered, and then fixing it.

[0019] In some embodiments, the thickness of the film is 0.008 mm, the width of the film is 2 to 3 cm, and the length of the film is 10 to 15 cm.

[0020] This application has the following beneficial effects:

[0021] (1) The seedling bud grafting method provided in the present application uses rubber tree leaf buds with petioles as scions for bud grafting with seedling rootstocks, thus filling the technical gap of seedling bud grafting with petiole leaf buds.

[0022] (2) The seedling bud grafting method of the present application, after removing the petiole, watering and fertilizing the next day can prevent moisture from entering the binding band and causing mildew, thereby improving the bud grafting survival rate and overcoming the current problem of low survival rate of seedlings when using leaf buds with petioles for bud grafting.

[0023] (3) The seedling bud grafting method of the present application does not require complicated processing of the scion buds, which can save the time of bud grafting.

[0024] (4) The seedling bud grafting method of the present application can remove the petiole in time after the petiole turns yellow. Not only can the petiole be removed by hand, but it can also prevent the petiole from rotting in the membrane, thereby affecting the success rate of bud grafting. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the technical solutions in the present disclosure, the following briefly introduces the drawings required to be used in some embodiments of the present disclosure. Obviously, the drawings described below are only drawings of some embodiments of the present disclosure. For ordinary technicians in this field, other drawings can also be obtained based on these drawings. In addition, the drawings described below can be regarded as schematic diagrams, and are not limitations on the actual process of the method involved in the embodiments of the present disclosure.

[0026] Figure 1 The process of taking bud pieces for the peeling method;

[0027] Figure 2 It is the process of removing petioles;

[0028] Figure 3 This is the petiole growth condition of the grafted rubber tree Reyan 879 scion bud after 10 to 14 days of routine management. DETAILED DESCRIPTION

[0029] The technical solutions in some embodiments of the present disclosure are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present disclosure, not all of the embodiments. Based on the embodiments provided by the present disclosure, all other embodiments obtained by ordinary technicians in this field belong to the scope of protection of the present disclosure.

[0030] Example 1

[0031] Follow these steps to bud your Hevea brasiliensis tree:

[0032] S1. Sowing the seeds of Hevea brasiliensis GT1 in a sand bed and cultivating them for 3 to 4 weeks to obtain seedling rootstocks with seeds;

[0033] S2. Select the buds of rubber tree Reyan 879 whose top leaves are in the stable to budding period, and use the stripping method (such as Figure 1 As shown in the figure, a bud piece of a leaf bud with a petiole of 29.22 mm in length and 7.34 mm in width is taken out, and the petiole is trimmed to 4.08 mm to obtain a scion bud piece;

[0034] S3. The seedling stock is pulled up from the sand bed, the root substrate is cleaned, and the flat surface young stem is selected, and the base is opened to 3 / 5 of the seedling stock stem circumference and 30 mm long. The large bud interface;

[0035] S4. The scion bud is placed on the large bud interface, and a film having a thickness of 0.008 mm, a width of 2 cm, and a length of 10 cm is used to bind from bottom to top. When the petiole is located, it is first wound from top to bottom for 2 turns, and then continued to be wound upward until the film completely covers the large bud interface to obtain a good budding seedling;

[0036] S5. Transplant the budded seedlings into a seedling cup filled with soil with an upper diameter of 8 cm* a lower diameter of 6 cm* a height of 30 cm, water them for rooting, and perform routine management for 10 days. During the routine management period, water them once every 2 days and fertilize them once every 7 days. The fertilizer applied is Lion Horse brand compound fertilizer N:P:K=15-15-15, and the fertilizer application rate is 1%, and budded seedlings with yellow buds and petioles are obtained;

[0037] S6. Cut open the plastic film at the base of the petiole of the budded seedlings, remove the petiole by hand (such as Figure 2 As shown), cover the leaf scars with a film, start watering and fertilizing after one day, and continue the cultivation and management for 6 months. During the cultivation and management, topping, bud removal, and untying are carried out. Water once every 2 days and fertilize once every 7 days to obtain seedlings and bud grafting.

[0038] Example 2

[0039] The steps of budding the Hevea brasiliensis in this embodiment refer to the first embodiment; wherein the difference between the second embodiment and the first embodiment is only that:

[0040] In S5, the duration of conventional management was 11 days.

[0041] Example 3

[0042] The steps of budding the Hevea brasiliensis in this embodiment refer to the first embodiment; wherein the difference between the third embodiment and the first embodiment is only that:

[0043] In S5, the duration of conventional management was 12 days.

[0044] Example 4

[0045] The steps of budding the Hevea brasiliensis in this embodiment refer to the first embodiment; wherein the difference between the fourth embodiment and the first embodiment is only that:

[0046] In S5, the duration of conventional management was 13 days.

[0047] Example 5

[0048] The steps of budding the Hevea brasiliensis in this embodiment refer to the embodiment 1; wherein the difference between the embodiment 5 and the embodiment 1 is only that:

[0049] In S5, the duration of conventional management is 14 days.

[0050] Embodiment 6:

[0051] The steps of budding the Hevea brasiliensis in this embodiment refer to Example 5; wherein, the difference between Example 6 and Example 5 is only that:

[0052] In S2, the bud piece of the petiolated leaf bud removed was 36.00 mm long and 9.11 mm wide, and the petiole was trimmed to 6.47 mm;

[0053] In S3, the width of the large bud interface is 7 / 10 of the circumference of the seedling rootstock stem and the length is 40 mm;

[0054] In S4, the film has a thickness of 0.008 mm, a width of 2.5 cm, and a length of 12.5 cm.

[0055] Example 7

[0056] The steps of budding the Hevea brasiliensis in this embodiment refer to Example 6; wherein, the difference between Example 7 and Example 6 is only that:

[0057] In S2, the rubber tree thermal research 879 is replaced by the cloud research 7346.

[0058] Example 8

[0059] The steps of budding the Hevea brasiliensis in this embodiment refer to Example 6; wherein, the difference between Example 8 and Example 6 is only that:

[0060] In S2, the rubber tree thermal research 879 is replaced by thermal reclamation 628.

[0061] Example 9

[0062] Follow these steps to bud your Hevea brasiliensis tree:

[0063] S1. Sowing the seeds of Hevea brasiliensis GT1 in a sand bed and cultivating them for 3 to 4 weeks to obtain seedling rootstocks with seeds;

[0064] S2. Select the buds of the top leaves of the rubber tree RP107 in the stable period to the budding period, remove the buds of the petiole buds with a length of 42.83 mm and a width of 10.87 mm by the stripping method, trim the petiole to 8.85 mm, and obtain the scion buds;

[0065] S3. The seedling stock is pulled up from the sand bed, the root substrate is cleaned, and the flat surface young stem is selected, and the base is opened to be 4 / 5 of the seedling stock stem circumference and 50 mm long. The large bud interface;

[0066] S4. The scion bud is placed on the large bud interface, and a film having a thickness of 0.008 mm, a width of 3 cm, and a length of 15 cm is used to bind from bottom to top. When the petiole is located, it is first wound from top to bottom for 2 turns, and then continued to be wound upward until the film completely covers the large bud interface to obtain a good budding seedling;

[0067] S5. Transplant the budded seedlings into a seedling cup filled with soil with an upper diameter of 8 cm* a lower diameter of 6 cm* a height of 30 cm, water them for rooting, and perform routine management for 10 days. During the routine management period, water them once every 2 days and fertilize them once every 7 days. The fertilizer applied is Lion Horse brand compound fertilizer N:P:K=15-15-15, and the fertilizer application rate is 1%, and budded seedlings with yellow buds and petioles are obtained;

[0068] S6. cut open the plastic film at the base of the petiole of the bud-grafted seedling, remove the petiole by hand, cover the leaf scar with the film, start watering and fertilizing after one day, and continue the cultivation and management for 6 months. During the cultivation and management, topping, bud removal, and unbinding are carried out, watering is carried out once every 2 days, and fertilizer is applied once every 7 days to obtain seedling bud-grafted seedlings.

[0069] Comparative Example 1

[0070] Follow these steps to bud your Hevea brasiliensis tree:

[0071] S1. Sowing the seeds of Hevea brasiliensis GT1 in a sand bed and cultivating them for 3 to 4 weeks to obtain seedling rootstocks with seeds;

[0072] S2. Select the top leaf of rubber tree Reyan 879 in the stable period to the budding period, remove the bud piece of the petiole bud with a length of 36.00 mm and a width of 9.11 mm by stripping, trim the petiole to 6.47 mm, and obtain the scion bud piece;

[0073] S3. The seedling stock is pulled up from the sand bed, the root substrate is cleaned, and the flat surface of the young stem is selected, and the base is opened with a width of 7 / 10 of the stem circumference of the seedling stock and a length of 40 mm. The large bud interface;

[0074] S4. The scion bud is placed on the large bud interface, and a film with a thickness of 0.008 mm, a width of 2.5 cm, and a length of 12.5 cm is used for bundling from bottom to top. When the petiole is located, it is first wound from top to bottom for 2 turns, and then continued to be wound upward until the film completely covers the large bud interface to obtain a good budding seedling;

[0075] S5. Transplant the grafted seedlings into a seedling cup filled with soil with an upper diameter of 8 cm* a lower diameter of 6 cm* a height of 30 cm, water them with enough water for rooting, and carry out routine management for 10 days. During the routine management period, water them once every 2 days and fertilize them once every 7 days. The fertilizer applied is Lion brand compound fertilizer N:P:K=15-15-15, and the fertilizer application rate is 1%. Stop watering and fertilizing for 1 day, and then continue to carry out cultivation and management for 6 months. During the cultivation and management period, topping, bud removal, and unbinding are carried out. Water them once every 2 days and fertilize them once every 7 days to obtain seedling grafted seedlings.

[0076] Comparative Example 2

[0077] The steps of budding the Hevea brasiliensis in this comparative example refer to comparative example 1; wherein, the difference between comparative example 2 and comparative example 1 is only that:

[0078] In S2, the rubber tree thermal research 879 is replaced by the cloud research 7346.

[0079] Comparative Example 3

[0080] The steps of budding the Hevea brasiliensis in this comparative example refer to comparative example 1; wherein, the difference between comparative example 3 and comparative example 1 is only that:

[0081] In S2, the rubber tree thermal research 879 is replaced by thermal reclamation 628.

[0082] Comparative Example 4

[0083] Follow these steps to bud your Hevea brasiliensis tree:

[0084] S1. Sowing the seeds of Hevea brasiliensis GT1 in a sand bed and cultivating them for 3 to 4 weeks to obtain seedling rootstocks with seeds;

[0085] S2. Select the buds of rubber tree RP107 with top leaves in the stable period to the budding period, and remove the buds of the scale buds with a length of 42.83 mm and a width of 10.87 mm by stripping to obtain the scion buds;

[0086] S3. The seedling stock is pulled up from the sand bed, the root substrate is cleaned, and the flat surface young stem is selected, and the base is opened to be 4 / 5 of the seedling stock stem circumference and 50 mm long. The large bud interface;

[0087] S4. The scion bud is placed on the large bud interface and is bundled from bottom to top with a film having a thickness of 0.008 mm, a width of 3 cm and a length of 15 cm until the film completely covers the large bud interface to obtain a good budding seedling;

[0088] S5. Transplant the grafted seedlings into a seedling raising cup filled with soil with an upper diameter of 8 cm* a lower diameter of 6 cm* a height of 30 cm, water them with enough water for rooting, and carry out routine management for 10 days. During the routine management period, water them once every 2 days and fertilize them every 7 days. Stop watering and fertilizing for 1 day, and then continue to carry out cultivation management for 6 months. During the cultivation management period, top the seedlings, remove the buds, and untie the seedlings. Water them once every 2 days and fertilize them once every 7 days to obtain grafted seedlings.

[0089] Comparative Example 5

[0090] The steps of budding the Hevea brasiliensis in this comparative example refer to comparative example 4; wherein, the difference between comparative example 5 and comparative example 4 is only that:

[0091] In S1, the number of the seedlings of the Hevea brasiliensis GT1 is 258;

[0092] In S2, there were 258 rubber trees RP107, and the scale buds were replaced with petiolate leaf buds.

[0093] Comparative Example 6

[0094] The steps of budding the Hevea brasiliensis in this comparative example refer to Example 9; wherein, the difference between Comparative Example 6 and Example 9 is only that:

[0095] In S6, watering and fertilization were carried out on the day when the petioles were taken.

[0096] Experimental Example 1: Bud grafting speed test

[0097] On the premise of preparing bud sticks and rootstocks, the scale buds and petiole-less leaf buds were obtained by the chipping method, and the leaf buds with petioles were obtained by the stripping method. Three bud grafters were selected to perform bud grafting operations on scale buds, petiole-less leaf buds, and petiole leaf buds for 1 hour respectively, and the number of bud graftings was recorded. The results are shown in Table 1.

[0098] Table 1:

[0099] Bud picks up people Scale bud De-petiolate leaf buds Leaf bud with petiole F-number A 93 87 92 B 112 106 107 C 79 74 83 Average 94.67 89.00 94.00 0.13

[0100] Note: *Significant difference at 5% level, **Significant difference at 1% level.

[0101] The results showed that, under the premise of preparing bud strips and rootstocks in advance, there was no significant difference in the budding speed between seedling budding using leaf buds with petioles and using scale buds, and the speed was faster than seedling budding using leaf buds without petioles.

[0102] Experimental Example 2: Effect of petiole removal time on survival rate

[0103] The saplings of Brazilian rubber tree were budded according to the methods of Examples 1 to 5, and the effects of petiole removal on the survival rate of budded seedlings after conventional management for different periods of time were statistically analyzed. The statistical results are shown in Table 2.

[0104] Table 2:

[0105] Routine management time / d Number of plants treated Number of surviving plants Survival rate 10 72 65 90.28％ 11 72 61 84.72％ 12 72 67 93.06％ 13 72 69 95.83％ 14 72 71 98.61％

[0106] During the experiment, it was found that after routine management on the 6th day, some petioles turned yellow. The larger the petiole, the slower the yellowing. On the 10th day, about 2 / 3 of the petioles turned yellow, and some small petioles turned black. By the 14th day, 5 / 6 of the petioles had turned black (such as Figure 3 The petioles were green at the base and difficult to fall off. The buds were easily injured during the process. Petioles were removed from the 10th day onwards, and 2 / 3 of the petioles could be easily removed. On the 14th day, a few petioles were found to have begun to rot.

[0107] Experimental Example 3: Comparison of survival rate of bud grafting with and without petiole removal

[0108] After budding of the Brazilian rubber trees according to the methods of Examples 6 to 8 and Comparative Examples 1 to 3, the survival rates of the budding of different varieties of rubber trees with and without petiole removal after conventional management were statistically analyzed. The statistical results are shown in Table 3.

[0109] Table 3:

[0110]

[0111]

[0112] The results showed that petiole removal after routine management could improve the survival rate of bud grafting.

[0113] Experimental Example 4: Comparison of budding survival rates of different bud types

[0114] After budding of the Brazilian rubber trees according to Example 9 and Comparative Examples 4-5, the survival of the budded seedlings of the rubber tree PR107 obtained by budding with different types of bud pieces was statistically analyzed, and the statistical results are shown in Table 4.

[0115] Table 4:

[0116]

[0117] Note: *Significant difference at 5% level, **Significant difference at 1% level.

[0118] The results show that the budding survival rate of leaf buds with petioles using the budding method provided by the present application is not significantly different from that of the budding method of scale buds and petiole-free leaf buds. In addition, it can be seen from Comparative Example 6 that on the basis of the budding method provided by the present application, even if the petiole is not removed after routine management, the survival rate of budded seedlings is much higher than that recorded in the seedling budding technology by the Rubber Research Institute of the South China Academy of Tropical Crops Sciences: the budding survival rate of leaf buds with petioles is 15% to 36%. This shows that the method provided by the present application can successfully overcome the problem of low survival rate of budding of leaf buds with petioles. .

[0119] In summary, the seedling bud grafting method of the brazilian rubber tree with petiole leaf buds provided by the present application has a bud grafting speed that is not much different from the bud grafting method of the scale buds commonly used in the art, and is faster than the bud grafting method of the petiole-free leaf buds. The survival rate of the seedlings after bud grafting is also very close to the bud grafting method of the scale buds and the bud grafting method of the petiole-free leaf buds. The seedling bud grafting method of the present application not only ensures the bud grafting speed, but also overcomes the current situation that the seedling bud grafting method with petiole leaf buds easily leads to a low survival rate of the seedlings, and provides a new technical route for the seedling bud grafting technology.

[0120] The above is only a preferred embodiment of the present disclosure. It should be understood that the present disclosure is not limited to the form disclosed herein, and should not be regarded as excluding other embodiments, but can be used in various other combinations, modifications and environments, and can be modified within the scope of the concept described herein through the above teachings or the technology or knowledge of the relevant field. The changes and modifications made by those skilled in the art do not deviate from the spirit and scope of the present disclosure, and should be within the scope of protection of the claims attached to the present disclosure.

Claims

1. A method for budding seedlings of Hevea brasiliensis with petiole leaf buds, characterized in that: The following steps are involved: Sowing the seeds of the Brazilian rubber tree in a sand bed to cultivate seedling rootstocks with seeds; Selecting buds of rubber trees for scion, removing bud pieces with petiole leaf buds, and pruning them to obtain scion bud pieces; Select the flat surface of the seedling rootstock and open an interface at the base; Bud grafting the scion bud piece onto the interface to obtain a bud grafted seedling; Transplanting the budded seedlings to obtain budded seedlings; as well as After performing routine management on the bud-grafted seedlings, the petioles are removed, the leaf scars are covered, watering and fertilizing are performed every other day, and cultivation and management are performed to obtain seedling bud-grafted seedlings; Wherein, the step of removing petioles comprises: Cut open the plastic film at the base of the petiole, remove the petiole by hand, and then cover the leaf scar with the film.

2. The seedling bud grafting method according to claim 1, characterized in that: The bud strips include bud strips whose canopy leaves are in the stable stage to the sprouting stage.

3. The seedling bud grafting method according to claim 1, characterized in that: The method for removing the petiole leaf buds comprises a stripping method.

4. The seedling bud grafting method according to claim 1, characterized in that: The length of the scion bud piece is 29.22-42.83 mm, the width of the scion bud piece is 7.34-10.87 mm, and the petiole length of the scion bud piece is 4.08-8.85 mm.

5. The seedling bud grafting method according to claim 1, characterized in that: The interface comprises a large bud interface, the width of the large bud interface is 3 / 5 to 4 / 5 of the circumference of the stem of the seedling rootstock, and the length of the large bud interface is 30 to 50 mm.

6. The seedling bud grafting method according to claim 1, characterized in that: The cultivation time is 3 to 4 weeks; And / or, the duration of the routine management is 10 to 14 days.

7. The seedling bud grafting method according to claim 1, characterized in that: The budding method comprises: Place the scion bud at the interface, and use a film to bind it from bottom to top. When it reaches the position of the petiole, first wrap it from top to bottom for 2 to 3 circles, and then continue to wrap it upward until the interface is completely covered and then fixed.

8. The seedling bud grafting method according to claim 9, characterized in that: The thickness of the film is 0.008 mm, the width of the film is 2 to 3 cm, and the length of the film is 10 to 15 cm.

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