Breeding system for combined breeding of zooplankton and phytoplankton and method for combined breeding of zooplankton and phytoplankton

Abstract

The invention belongs to the technical field of aquaculture, and particularly relates to a breeding system for combined breeding of zooplankton and phytoplankton and a method for combined breeding of zooplankton and phytoplankton. By means of the breeding system, the phytoplankton releases oxygen and generates organic matter through photosynthesis, and abundant bait is provided for the zooplankton; the zooplankton is fed by the phytoplankton, nutritive salt in the generated waste is dissolved in water and becomes a nutrition source of the phytoplankton, high-density growth of the zooplankton and the phytoplankton is achieved in a limited breeding space, and land resources are effectively saved; and in combination with an intelligent monitoring system, water body environment parameters can be monitored and adjusted in real time, plankton is ensured to grow under the most suitable environment condition, the feed conversion efficiency is improved, and water body pollution is effectively reduced. The primer aquatic bacillus can increase dissolved oxygen in a water body, promote decomposition of organic matters in the water body and release of inorganic nutritive salt, and further support growth of phytoplankton.

Description

Technical Field

[0001] The invention belongs to the technical field of aquaculture, and in particular relates to a culture system for combined culture of zooplankton and phytoplankton and a method for combined culture of zooplankton and phytoplankton. Background Art

[0002] Against the backdrop of the continuous growth of the global population and the rising demand for aquatic products, how to achieve ecological and efficient aquaculture while ensuring the quality of aquatic products has become a technical problem that needs to be solved urgently. Green and healthy aquaculture models have gradually become the mainstream trend of industry development, and the market demand for high-quality aquatic products is also increasing. Live feed, as an important component of green feed, has received widespread attention and favor in aquaculture, especially as a starter feed for young fish, because of its advantages such as rich nutrition and easy digestion and absorption. However, traditional live feed farming methods have many limitations and challenges. On the one hand, the aquaculture output of traditional methods is relatively low, which is difficult to meet the needs of large-scale aquaculture; on the other hand, due to factors such as large land area and limited transportation, the large-scale and standardized production of live feed faces many difficulties, which seriously restricts the improvement of production efficiency and is difficult to meet the urgent needs of modern aquaculture for efficient and stable feed sources. Summary of the invention

[0003] The purpose of the present invention is to make up for the deficiencies of the prior art, to provide a breeding system for the combined breeding of zooplankton and phytoplankton and a method for the combined breeding of zooplankton and phytoplankton, to construct an ecological cycle chain of coupled zooplankton and phytoplankton, to improve feed conversion efficiency, and to effectively reduce water pollution.

[0004] The present invention provides a breeding system, including a phytoplankton breeding area, a zooplankton breeding area, a culture solution regulating system, a carbon dioxide supply system, an intelligent monitoring system and a filtering system;

[0005] The bottom surface of the zooplankton culture area is a slope structure;

[0006] The water outlet pipelines of the zooplankton culture area and the phytoplankton culture area are connected;

[0007] The water outlet of the zooplankton culture area is connected to the water inlet of the phytoplankton culture area through the filtration system;

[0008] The outlets of the culture solution regulating system and the carbon dioxide supply system are respectively connected to the water inlet of the zooplankton breeding area;

[0009] A sewage discharge system and a valve control system are arranged in the zooplankton breeding area.

[0010] Preferably, the phytoplankton cultivation area is provided with a photoreactor pipeline.

[0011] Preferably, the culture solution regulating system and the carbon dioxide supply system are respectively connected to the intelligent monitoring system pipelines.

[0012] Preferably, the intelligent monitoring system is provided with a sensor system, a data processing system and an automatic control device.

[0013] The present invention also provides the application of the aquaculture system described in the above technical solution in the combined aquaculture of zooplankton and phytoplankton.

[0014] The present invention also provides a method for jointly cultivating zooplankton and phytoplankton based on the aquaculture system described in the above technical solution, comprising the following steps:

[0015] In the phytoplankton cultivation area, when the density of phytoplankton reaches 0.2×10 7 ~2.6×10 7 When the concentration of zooplankton reaches 100 / L, zooplankton is inoculated into the zooplankton breeding area to form a closed ecological cycle chain of zooplankton and phytoplankton. The water environment is monitored in real time using an intelligent monitoring system, and the breeding conditions are adjusted to ensure the reproduction of phytoplankton and zooplankton.

[0016] Preferably, during the reproduction of phytoplankton and zooplankton, the pH of the water body is 7.2-8.5, the dissolved oxygen is 4-6 mg / L, the temperature is 18-28° C., and the nutrient salt concentration is 17-25 mg / L.

[0017] Preferably, the nutrient salt includes TN and TP; the concentration of TN is 15-20 mg / L, and the concentration of TP is 2-5 mg / L.

[0018] Preferably, the zooplankton includes one or more of rotifers, cladocerans and copepods;

[0019] The phytoplankton includes Chlorophyta.

[0020] Preferably, the method further comprises: inoculating aquatic Bacillus in the aquaculture water.

[0021] Beneficial effects:

[0022] The present invention provides a breeding system, including a phytoplankton breeding area, a zooplankton breeding area, a culture solution regulating system, a carbon dioxide supply system, an intelligent monitoring system and a filtering system; the bottom surface of the zooplankton breeding area is a slope structure; the zooplankton breeding area is connected to the water outlet pipeline of the phytoplankton breeding area; the water outlet of the zooplankton breeding area is connected to the water inlet of the phytoplankton breeding area through the filtering system; the outlets of the culture solution regulating system and the carbon dioxide supply system are respectively connected to the water inlet of the zooplankton breeding area; the zooplankton breeding area is provided with a sewage system and a valve control system. The breeding system of the present invention can be used to breed phytoplankton and zooplankton, and a coupled ecological chain of zooplankton and phytoplankton can be constructed. Phytoplankton releases oxygen and produces organic matter through photosynthesis, providing abundant bait for zooplankton; zooplankton feeds on phytoplankton, and the nutrients in its excrement and residues are dissolved in water, which becomes a nutrient source for phytoplankton. This coupling relationship can achieve high-density growth of zooplankton and phytoplankton in a limited breeding space, which not only increases the yield per unit area, but also effectively saves land resources; high-density breeding technology combined with an intelligent monitoring system can monitor and adjust water environmental parameters in real time to ensure that plankton grows under the most suitable environmental conditions, which not only improves feed conversion efficiency but also effectively reduces water pollution.

[0023] Furthermore, the present invention can induce aquatic spores during the cultivation of phytoplankton and zooplankton, inhibit cyanobacteria, dinoflagellates, and naked algae, reduce organic waste, promote photosynthesis of beneficial algae (chlorophyte species such as Chlorella, etc.), increase dissolved oxygen in water, promote the decomposition of organic matter in the water and the release of inorganic nutrients, and further support the growth of phytoplankton. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments are briefly introduced below.

[0025] Figure 1 and Figure 2 A three-dimensional structural diagram of a breeding system provided in Example 1 of the present invention;

[0026] Figure 3 A top view of the breeding system provided in Example 1 of the present invention;

[0027] In the figure: 1-culture solution regulating tank, 2-photoreactor tube, 3-animal breeding pool, 4-intelligent monitoring system, 5-carbon dioxide supply system, 6-filter, 7-pump, 8-drainage outlet, 9-valve. DETAILED DESCRIPTION

[0028] The present invention provides a breeding system, including a phytoplankton breeding area, a zooplankton breeding area, a culture solution regulating system, a carbon dioxide supply system, an intelligent monitoring system and a filtering system;

[0029] The bottom surface of the zooplankton culture area is a slope structure;

[0030] The water outlet pipelines of the zooplankton culture area and the phytoplankton culture area are connected; a valve control system is provided on the pipeline;

[0031] The water outlet of the zooplankton culture area is connected to the water inlet of the phytoplankton culture area through the filtration system;

[0032] The outlets of the culture solution regulating system and the carbon dioxide supply system are respectively connected to the water inlet of the zooplankton breeding area;

[0033] A sewage discharge system is arranged in the zooplankton breeding area.

[0034] The aquaculture system of the present invention includes a phytoplankton aquaculture area. As an embodiment, the phytoplankton aquaculture area of ​​the present invention is provided with a photoreactor pipe. As an embodiment, the photoreactor pipe of the present invention is made of a transparent material and is equipped with a lamp with adjustable light intensity. The present invention utilizes a photoreactor pipe to cultivate plants, which can provide suitable light conditions for plants, especially phytoplankton, promote their photosynthesis and reproduction, realize efficient cultivation of plants, and provide sufficient bait for subsequent animals.

[0035] The aquaculture system described in the present invention includes a zooplankton aquaculture area; a sewage system is provided on the zooplankton aquaculture area. The zooplankton aquaculture area described in the present invention is connected to the outlet pipeline of the phytoplankton aquaculture area, forming a closed ecological cycle chain of zooplankton and phytoplankton coupling, which is convenient for animals (especially zooplankton) to feed on plants, provides a suitable living environment for animals, promotes their growth and reproduction, improves feed conversion efficiency and reduces water pollution. A valve control system is provided on the pipeline described in the present invention to control the speed at which the water flow from the phytoplankton aquaculture area enters the zooplankton aquaculture area. The bottom surface of the zooplankton aquaculture area pool described in the present invention is a slope structure, which can collect solid waste generated during the aquaculture process into the sewage system.

[0036] The water outlet of the zooplankton culture area of ​​the present invention is connected with the water inlet of the phytoplankton culture area through the filtration system. The filtration system of the present invention can keep the water clean, improve the self-purification ability of the water body, and provide a good growth environment for plants and animals.

[0037] The aquaculture system of the present invention includes an intelligent monitoring system. As an embodiment, the intelligent monitoring system of the present invention is provided with a sensor system, a data processing system and an automatic control device, which can monitor water environment parameters in real time, predict the growth trend of plankton, and automatically adjust the aquaculture conditions to achieve precise aquaculture and scientific management, and improve aquaculture efficiency and product quality.

[0038] The aquaculture system of the present invention comprises a culture solution regulating system and a carbon dioxide supply system; the outlets of the culture solution regulating system and the carbon dioxide supply system are respectively connected to the water inlet of the zooplankton aquaculture area.

[0039] As an embodiment, the aquaculture system of the present invention further includes a collection system. The present invention utilizes the collection system to collect live feed in the zooplankton aquaculture area, wastewater excrement and residual organic matter generated during the aquaculture process. The collected live feed can be directly used in aquaculture to achieve continuous culture, and can also be used as a starter feed for other aquatic animals; the wastewater and residual organic matter generated during the aquaculture process can be used as fertilizer or reintroduced into the aquaculture system for recycling after being treated, thereby maximizing the utilization of resources.

[0040] In the aquaculture system provided by the present invention, the zooplankton aquaculture area and the phytoplankton aquaculture area are coupled to form a closed ecological cycle chain, which can achieve high-density growth of animals and plants in a limited space; the water environment parameters are monitored in real time by an intelligent monitoring system, and the aquaculture conditions are automatically adjusted according to the monitoring results to ensure that animals and plants reproduce in the best growth state, improve feed conversion efficiency and reduce water pollution.

[0041] In view of the advantages of the aquaculture system provided by the present invention, the application of the aquaculture system in the combined aquaculture of zooplankton and phytoplankton also falls within the protection scope of the present invention.

[0042] The present invention also provides a method for co-culturing zooplankton and phytoplankton based on the aquaculture system described in the above technical solution, comprising the following steps:

[0043] The phytoplankton culture area is used for phytoplankton culture. When the density of phytoplankton reaches 0.2×10 7 ~2.6×10 7 When the concentration of zooplankton reaches 10000 tons / L, zooplankton is inoculated into the zooplankton breeding area to form a closed ecological cycle chain of zooplankton and phytoplankton. The intelligent monitoring system is used to monitor the water environment in real time, adjust the breeding conditions, and ensure that phytoplankton and zooplankton reproduce in the best growth state.

[0044] The present invention utilizes a phytoplankton culture area to culture phytoplankton. As an embodiment, the phytoplankton of the present invention includes Chlorophyta; as another embodiment, the phytoplankton of the present invention is Chlorella. As an embodiment, the light intensity of the phytoplankton culture of the present invention is 4500-8000lx; as another embodiment, the light intensity of the phytoplankton culture of the present invention is 5000-7000lx; as another embodiment, the light intensity of the phytoplankton culture of the present invention is 6000lx. The present invention has no strict requirements on the culture method of the phytoplankton culture, and it is sufficient to ensure the temperature and nutrient concentration required for the growth of phytoplankton and make the phytoplankton reproduce rapidly. Phytoplankton can release oxygen through photosynthesis to provide a necessary living environment for other organisms in the water body.

[0045] As an embodiment, the present invention inoculates aquatic Bacillus in phytoplankton culture water. As an embodiment, the inoculation amount of aquatic Bacillus is 1-2 g / m 3 Aquaculture water. As an embodiment, the aquatic Bacillus is Bacillus subtilis. The present invention has no strict requirements on the source of the aquatic Bacillus, and it can be purchased conventionally. The aquatic Bacillus used in the embodiment of the present invention was purchased from Yiqiang Biology. The addition of aquatic Bacillus in the present invention can further promote the decomposition of organic matter and the release of inorganic nutrients in the aquaculture water, further support the growth of phytoplankton, improve the water environment, and enhance the self-purification ability of the water.

[0046] In the present invention, when the density of phytoplankton reaches 0.2×10 7 ~2.6×10 7 When the density of phytoplankton reaches 0.5×10 7 ~2.0×10 7 When the density of phytoplankton reaches 1.0×10 7 ~1.5×10 7 When the concentration of zooplankton is 1.0477 g / L, zooplankton is inoculated into the zooplankton culture area. The closed ecological cycle chain between zooplankton and phytoplankton reduces the accumulation of organic waste in the water, reduces the risk of eutrophication of the water, and protects the culture environment.

[0047] As an embodiment, the zooplankton of the present invention includes one or more of rotifers, cladocerans and copepods; as another embodiment, the zooplankton of the present invention includes rotifers, cladocerans and copepods. As an embodiment, the zooplankton of the present invention is the zooplankton after 7 days of indoor culture. As an embodiment, the indoor culture method is: inoculating the zooplankton into aerated tap water and feeding bait. As an embodiment, the bait includes Chlorella and / or yeast. As an embodiment, the inoculation density of the zooplankton is 100 to 500 / L; as another embodiment, the inoculation density of the zooplankton is 200 to 400 / L; as another embodiment, the inoculation density of the zooplankton is 300 / L.

[0048] In the process of joint cultivation of zooplankton and phytoplankton, the present invention uses an intelligent monitoring system to monitor the water environment in real time, adjust the cultivation conditions, and ensure that phytoplankton and zooplankton reproduce in the best growth state. As an embodiment, the pH of the water body under the optimal growth state is 7.2-8.5; as another embodiment, the pH of the water body under the optimal growth state is 7.5-8.0; as another embodiment, the pH of the water body under the optimal growth state is 7.8. As an embodiment, the dissolved oxygen of the water body under the optimal growth state is 4-6 mg / L; as another embodiment, the dissolved oxygen of the water body under the optimal growth state is 5 mg / L. As an embodiment, the temperature of the water body under the optimal growth state is 18-28°C. As another embodiment, the temperature of the water body under the optimal growth state is 25°C. As an embodiment, the nutrient salt concentration of the water body under the optimal growth state is 17-25 mg / L; as another embodiment, the nutrient salt concentration of the water body under the optimal growth state is 22 mg / L. As an embodiment, the nutrient salt includes TN and TP; the concentration of TN is 15-20 mg / L, and the concentration of TP is 2-5 mg / L; as another embodiment, the concentration of TN is 18 mg / L, and the concentration of TP is 4 mg / L.

[0049] The method for co-culturing zooplankton and phytoplankton provided by the present invention adopts high-density cultivation technology and modular design concept to construct a cultivation system, realizes high-yield cultivation in a limited space, enhances the flexibility and scalability of the system, not only increases the yield per unit area, but also effectively saves land resources, improves land utilization efficiency and the success rate of cultivation, and reduces the cost of cultivation. The method provided by the present invention can achieve high-yield and high-quality production of live feed, and significantly improves the efficiency of cultivation and resource utilization.

[0050] In order to further illustrate the present invention, a culture system for the combined culture of zooplankton and phytoplankton and a method for the combined culture of zooplankton and phytoplankton provided by the present invention are described in detail below in conjunction with the accompanying drawings and embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0051] Example 1

[0052] like Figure 1 to Figure 3 As shown, this embodiment provides a breeding system for the joint breeding of zooplankton and phytoplankton, including a culture fluid regulating tank (culture fluid regulating system) 1, a photoreactor tube (phytoplankton breeding area) 2, a breeding pond (zooplankton breeding area) 3, an intelligent monitoring system 4, a carbon dioxide supply system 5, a filter 6 and a pump 7; the breeding pond is provided with a sewage outlet (sewage system) 8 and a valve (valve control system) 9.

[0053] Example 2

[0054] The breeding system provided in Example 1 is used for breeding, which specifically includes the following steps:

[0055] 1. Indoor culture of zooplankton

[0056] Prepare containers (buckets, aquariums, etc.), culture water (aerated tap water) and bait (Chlorella and yeast) for culturing zooplankton (rotifers, cladocerans, copepods, etc.); inoculate zooplankton into the culture water at an inoculation density of 100 to 500 per L and culture for 7 days to obtain zooplankton seedlings.

[0057] 2. Environmental preparation stage

[0058] Clean and disinfect the breeding pond to ensure that the breeding environment is sterile and harmless. Add 1-2g of aquatic Bacillus (Bacillus) (purchase Yiqiang Biological) to each cubic meter of water to inhibit cyanobacteria, dinoflagellates, and euglena, reduce organic waste, promote photosynthesis of beneficial algae (chlorophytes such as Chlorella), and increase dissolved oxygen in the water. Improve the water environment by placing Bacillus subtilis and other control technologies, improve the self-purification capacity of the water and the growth conditions of phytoplankton.

[0059] 3. Constructing an ecological chain of zooplankton and phytoplankton coupling

[0060] When the phytoplankton reaches 0.2×10 7 ~2.6×10 7 After the density reaches 1 / L, the zooplankton seedlings cultured indoors in step 1 are introduced. These zooplankton feed on phytoplankton. The nutrients in the zooplankton's excrement and residues dissolve in water and become a source of nutrition for phytoplankton, forming a closed ecological cycle chain coupled with zooplankton and phytoplankton, which improves feed conversion efficiency and reduces water pollution.

[0061] 4. High-density farming and intelligent regulation

[0062] High-density aquaculture technology is used to achieve high-density growth of zooplankton and phytoplankton in a limited space. The intelligent monitoring system monitors water environmental parameters (such as dissolved oxygen, pH value, temperature, nutrient concentration, etc.) in real time, and automatically adjusts the aquaculture conditions according to the monitoring results. The intelligent control system adjusts the optimal growth environment of zooplankton and phytoplankton based on the data fed back by the sensors, pH (7.2-8.5), dissolved oxygen (4-6 mg / L), and online chlorophyll detectors to monitor the growth trend and density changes of microalgae, regulate the water flow rate of the photoreactor, accurately adjust the delivery of nutrients, light intensity, etc., to ensure that the biomass of phytoplankton is 100-380 μg / L, and ensure that zooplankton and phytoplankton reproduce in the best growth state.

[0063] 5. Harvest and Recycle

[0064] This embodiment is a continuous culture method for zooplankton and phytoplankton. The live feed is collected by a harvesting device and can be directly used for aquaculture or as a starter feed for other aquatic animals. The wastewater and residual organic matter generated during the breeding process can be used as fertilizer or reintroduced into the breeding system for recycling after treatment, thereby maximizing the utilization of resources.

[0065] According to the above content, it can be seen that the technical solution provided by the present invention can construct a coupled ecological chain of zooplankton and phytoplankton. Phytoplankton releases oxygen and produces organic matter through photosynthesis, providing rich bait for zooplankton; zooplankton feeds on phytoplankton, and the nutrients in its excrement and residues dissolve in water, becoming a source of nutrition for phytoplankton. This coupled relationship not only improves feed conversion efficiency, but also effectively reduces water pollution.

[0066] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A breeding system, characterized in that: It includes phytoplankton culture area, zooplankton culture area, culture medium regulation system, carbon dioxide supply system, intelligent monitoring system and filtration system; The bottom surface of the zooplankton culture area is a slope structure; The water outlet pipelines of the zooplankton culture area and the phytoplankton culture area are connected; a valve control system is provided on the pipeline; The water outlet of the zooplankton culture area is connected to the water inlet of the phytoplankton culture area through the filtration system; The outlets of the culture solution regulating system and the carbon dioxide supply system are respectively connected to the water inlet of the zooplankton breeding area; A sewage discharge system is arranged in the zooplankton breeding area.

2. The aquaculture system according to claim 1, characterized in that: The phytoplankton cultivation area is provided with a photoreactor pipeline.

3. The aquaculture system according to claim 1, characterized in that: The culture solution regulating system and the carbon dioxide supply system are respectively connected to the pipelines of the intelligent monitoring system.

4. The aquaculture system according to claim 1, characterized in that: The intelligent monitoring system is provided with a sensor system, a data processing system and an automatic control device.

5. Use of the aquaculture system according to any one of claims 1 to 4 in the combined aquaculture of zooplankton and phytoplankton.

6. A method for co-culturing zooplankton and phytoplankton based on the aquaculture system according to any one of claims 1 to 4, characterized in that: The steps include: In the phytoplankton cultivation area, when the density of phytoplankton reaches 0.2×10 7 ~2.6×10 7 When the concentration of zooplankton reaches 100 / L, zooplankton is inoculated into the zooplankton breeding area to form a closed ecological cycle chain of zooplankton and phytoplankton. The water environment is monitored in real time using an intelligent monitoring system, and the breeding conditions are adjusted to ensure the reproduction of phytoplankton and zooplankton.

7. The method according to claim 8, characterized in that During the reproduction of the phytoplankton and zooplankton, the pH of the water body is 7.2-8.5, the dissolved oxygen is 4-6 mg / L, the temperature is 18-28° C., and the nutrient salt concentration is 17-25 mg / L.

8. The method according to claim 7, characterized in that The nutrient salts include TN and TP; the concentration of TN is 15-20 mg / L, and the concentration of TP is 2-5 mg / L.

9. The method according to claim 6, characterized in that The zooplankton includes one or more of rotifers, cladocerans and copepods; The phytoplankton includes Chlorophyta.

10. The method according to any one of claims 6 to 9, characterized in that: Also includes: The aquaculture water was inoculated with Bacillus aquatica.

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