Extraction process of flos carthami extract and application of flos carthami extract in preparation of medicine for preventing cardiovascular and cerebrovascular diseases

Abstract

The invention discloses an extraction process of a safflower extract and application of the safflower extract in preparation of drugs for preventing cardiovascular and cerebrovascular diseases, a water extraction process is adopted to extract safflower, ascorbic acid and Tween 20 are added in the water extraction process, and the ascorbic acid and Tween 20 form a strong hydrogen bond network, so that the safflower extract can be used for treating cardiovascular and cerebrovascular diseases. The solubility of the hydroxysafflor yellow a and the dehydrated safflor yellow b can be improved; meanwhile, peptococcus betulinus, streptococcus thermophilus and saccharomycetes are adopted for treating the filter residues, the peptococcus betulinus can secrete cellulase and xylanase and can degrade cellulose and hemicellulose in cell walls, the streptococcus thermophilus can generate high-stability cellulase, degradation of the cell walls is further promoted, and the cell walls can be effectively degraded; according to the present invention, the yeast is added to the filter residue so as to easily release the active components in the filter residue into the water, and the yeast can produce a variety of metabolites such as alcohols, acids and esters during the fermentation process, such that the substances can be adopted as the solvent to help the dissolution of the active components in the filter residue so as to further improve the extraction rate;

Description

Technical Field

[0001] The invention relates to the technical field of separation of plant active ingredients, and in particular to an extraction process of safflower extract and an application of the extract in preparing drugs for preventing cardiovascular and cerebrovascular diseases. Background Art

[0002] Safflower (Carthamus tinctorius L.) is a plant belonging to the genus Carthamus of the Asteraceae family. Its dried flowers are widely used in traditional Chinese medicine in my country and have a long history and rich medicinal value. Safflower not only occupies an important position in my country's traditional Chinese medicine system, but is also gradually valued by modern medicine and pharmacological research. Clinical and pharmacological studies in recent years have shown that safflower has a significant regulatory effect on the cardiovascular, nervous and immune systems.

[0003] Safflower is believed to have the dual effects of promoting blood circulation and menstruation, dispersing blood stasis and relieving pain. It is often used to treat a variety of diseases related to blood circulation, especially dysmenorrhea, blood stasis and amenorrhea, abdominal pain due to postpartum blood stasis, traumatic injuries, and joint pain. In addition, safflower also plays an important role in the treatment of cardiovascular diseases, such as coronary heart disease and angina pectoris; common Chinese patent medicines on the market, such as Danhong Injection and Guhong Injection, all use safflower as the main ingredient, and their efficacy is widely recognized.

[0004] The medicinal effects of safflower mainly come from its rich active ingredients, especially total flavonoids and safflower yellow compounds. The chemical composition of safflower is complex and diverse, including flavonoids, alkaloids, sesquiterpenes, organic acids and polyacetylene compounds. Among them, hydroxysafflower yellow A and kaempferol are the ingredients mentioned more frequently in research and are specified as quality markers in the "Chinese Pharmacopoeia" (2015 edition). Among the specific requirements, the content of hydroxysafflower red A (C27H32O16) shall not be less than 1.0% (dry product), and the content of kaempferol shall not be less than 0.050% (dry product); these ingredients not only reflect the medicinal properties of safflower, but also provide standards for the control of its medicinal quality.

[0005] The active ingredients of traditional Chinese medicine are diverse and complex, which brings challenges to extraction and separation. In the production process of traditional Chinese medicine, extraction and separation is a crucial link, which is directly related to the quantity and quality of the active ingredients, and thus affects the stability and effectiveness of the product. For the extraction and preparation of safflower, the traditional water extraction method often faces problems such as low efficiency, difficult quality control, and easy deterioration.

[0006] Chinese patent document CN201410445632.9 discloses a preparation method and application of safflower extract. The extract is prepared according to the following method: safflower is subjected to enzyme fermentation and ultrasound-assisted extraction, macroporous adsorption resin purification, drying and other steps to refine the safflower extract. The main active ingredient of the safflower extract is safflower yellow, and it also contains a small amount of quercetin, etc.; however, the extraction rate of the active ingredients of safflower in this method is low and still needs to be further improved. Summary of the invention

[0007] In view of the deficiencies in the prior art, the purpose of the present invention is to provide an application of safflower extract in the preparation of drugs for preventing cardiovascular and cerebrovascular diseases. The extraction process of safflower extract provided by the present invention has the characteristics of high extraction efficiency.

[0008] In order to achieve the above object, the present invention adopts the following technical scheme:

[0009] An extraction process of safflower extract comprises the following steps:

[0010] S1, adding safflower to an aqueous solution containing ascorbic acid and Tween 20, heating and stirring to extract, and filtering after the extraction to obtain a first filtrate and a filter residue;

[0011] S2, dispersing the filter residue in deionized water, then adding composite bacteria, culturing for 5-7 days, sterilizing, heating, extracting, filtering, and obtaining a second filtrate;

[0012] S3. The first filtrate and the second filtrate are mixed and concentrated to obtain a safflower extract.

[0013] Specifically, in step S1, the dosage ratio of safflower and aqueous solution is 1g:10-20mL. In some embodiments of the present invention, for example, 1g:10mL, 1g:12mL, 1g:14mL, 1g:15mL, 1g:16mL, 1g:18mL, 1g:20mL can be selected; but it is not limited to the listed values, and other values ​​not listed within the numerical range are also applicable.

[0014] Among them, the mass fraction of ascorbic acid in the aqueous solution is 1-3%, for example, 1%, 1.5%, 2%, 2.5%, and 3% can be selected; the mass fraction of Tween 20 is 0.8-1.5%, for example, 0.8%, 0.9%, 1.0%, 1.1%, 1.2%, 1.4%, and 1.5% can be selected; but it is not limited to the listed values, and other values ​​not listed within the numerical range are also applicable.

[0015] Specifically, in step S1, the temperature for heating, stirring and leaching is 40-60°C, for example, 40°C, 45°C, 50°C, 55°C, and 60°C can be selected; the time for heating, stirring and leaching is 2-3h, for example, 2h, 2.5h, and 3h can be selected; but it is not limited to the listed values, and other values ​​not listed within the numerical range are also applicable.

[0016] Specifically, in step S2, the mass ratio of the filter residue to the composite bacteria is 100:0.2-0.5, for example, 100:0.2, 100:0.3, 100:0.4, 100:0.5 can be selected, but it is not limited to the listed values, and other values ​​not listed within the numerical range are also applicable.

[0017] The composite bacteria consists of birch tube-stripping bacteria, thermophilic streptococci and yeast, and the mass ratio of birch tube-stripping bacteria, thermophilic streptococci and yeast is 2.4-2.8:0.6-0.9:1.2-1.5.

[0018] Specifically, in step S2, the culture temperature is 25-28°C, for example, 25°C, 26°C, 27°C, 28°C can be selected, but it is not limited to the listed values, and other values ​​not listed within the numerical range are also applicable.

[0019] Specifically, in step S2, the temperature of the heating extraction is 50-60°C, for example, 50°C, 52°C, 55°C, 58°C, and 60°C can be selected; the time of the heating extraction is 1-2h, for example, 1h, 1.5h, and 2h can be selected, but it is not limited to the listed values, and other values ​​not listed within the numerical range are also applicable.

[0020] Specifically, in step S3, the specific process of concentration is: using rotary evaporation concentration, the rotary evaporation temperature is 50-60°C, the pressure is 0.01-0.03Mpa, and the concentration is performed until the ratio of the mass of the filtrate to the mass of the safflower added in step S1 is 1:1-1.1, so as to obtain the safflower extract.

[0021] The present invention provides a safflower extract prepared by the above preparation method.

[0022] The present invention also provides the use of the safflower extract in preparing medicines for preventing cardiovascular and cerebrovascular diseases.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] (1) The present invention adopts a water extraction process to extract the active ingredients in safflower. During the water extraction process, ascorbic acid and Tween 20 are added. Ascorbic acid, as an antioxidant, can effectively remove free radicals generated during the extraction process and protect the active ingredients in safflower from being easily oxidized and degraded. Tween 20 is a surfactant that can reduce the surface tension of the liquid and promote the rupture of the cell membrane, thereby promoting the effective transfer of ascorbic acid, protecting the released active ingredients and preventing them from being oxidized or degraded during the extraction process. At the same time, ascorbic acid and Tween 20 form a strong hydrogen bond network, which can improve the solubility of hydroxysafflower yellow a and dehydrated safflower yellow b in the aqueous phase system, thereby improving the extraction rate of the active ingredients in safflower.

[0025] (2) The present invention uses birch tube-exfoliating bacteria, thermophilic streptococci and yeast to treat the filter residue. The birch tube-exfoliating bacteria can secrete cellulase and xylanase, which can degrade cellulose and hemicellulose in the cell wall. The thermophilic streptococci can produce highly stable cellulase, which further promotes the degradation of the cell wall, making it easier for the active ingredients in the filter residue to be released into the water. At the same time, the yeast can produce a variety of metabolites during the fermentation process, such as alcohols, acids and esters. These substances can be used as solvents to help dissolve the active ingredients in the filter residue, further improving the extraction rate. In the present invention, the enzymatic degradation of birch tube-exfoliating bacteria and thermophilic streptococci is combined with the metabolic products of yeast to jointly improve the extraction rate of the active ingredients in the filter residue. DETAILED DESCRIPTION

[0026] The present invention is further described in detail below through specific preferred embodiments, but the present invention is not limited to the following embodiments.

[0027] It should be noted that, unless otherwise specified, the chemical reagents involved in the present invention were purchased through commercial channels.

[0028] The safflower used in the embodiment of the present invention was purchased from Huaxiang Qiaojun brand flagship store. The safflower originates from Xinjiang and is ground and passed through an 80-mesh sieve for later use.

[0029] The birch tube-stripping fungus was freeze-dried powder purchased from Shanghai Yiyan Biotechnology Co., Ltd. with the product number EY-1113J and the viable cell count was 1.0×10 8 CFU / g;

[0030] Streptococcus thermophilus was freeze-dried powder purchased from Zhejiang Yicun Biotechnology Co., Ltd., with a viable count of 1.0×10 6 CFU / g;

[0031] The yeast was freeze-dried powder purchased from Zhejiang Yicun Biotechnology Co., Ltd., with a viable count of 1.0×10 6 CFU / g.

[0032] Example 1

[0033] An extraction process of safflower extract comprises the following steps:

[0034] S1, adding 10g of safflower to 150mL of an aqueous solution containing ascorbic acid and Tween 20, wherein the mass fraction of ascorbic acid in the aqueous solution is 2%, and the mass fraction of Tween 20 is 1%, heating and stirring at 50°C for 2.5h, filtering after the extraction to obtain a first filtrate and a filter residue;

[0035] S2, dispersing the filter residue in 150 mL of deionized water, and then adding composite bacteria, wherein the mass ratio of the filter residue to the composite bacteria is 100:0.4, and the mass ratio of birch tube exfoliation bacteria, thermophilic Streptococcus and yeast in the composite bacteria is 2.5:0.8:1.2, culturing at a constant temperature of 28°C for 5 days, then sterilizing, heating and extracting at 50°C for 2 hours, filtering, and obtaining a second filtrate;

[0036] S3. The first filtrate and the second filtrate are mixed and concentrated by rotary evaporation at a rotary evaporation temperature of 50° C. and a pressure of 0.01 MPa. The concentration is performed until the ratio of the mass of the filtrate to the mass of safflower added in step S1 is 1:1, thereby obtaining a safflower extract.

[0037] Example 2

[0038] An extraction process of safflower extract comprises the following steps:

[0039] S1, adding 10g of safflower to 200mL of an aqueous solution containing ascorbic acid and Tween 20, wherein the mass fraction of ascorbic acid in the aqueous solution is 1%, and the mass fraction of Tween 20 is 1.5%, heating and stirring at 40°C for 3h, filtering after the extraction to obtain a first filtrate and a filter residue;

[0040] S2, dispersing the filter residue in 150 mL of deionized water, and then adding composite bacteria, wherein the mass ratio of the filter residue to the composite bacteria is 100:0.3, and the mass ratio of birch tube stripping bacteria, thermophilic Streptococcus and yeast in the composite bacteria is 2.4:0.6:1.5, culturing at 28°C for 7 days, then sterilizing, heating and extracting at 60°C for 1 hour, filtering, and obtaining a second filtrate;

[0041] S3. The first filtrate and the second filtrate are mixed and concentrated by rotary evaporation at a rotary evaporation temperature of 60° C. and a pressure of 0.01 MPa. The concentration is performed until the ratio of the mass of the filtrate to the mass of safflower added in step S1 is 1:1, thereby obtaining a safflower extract.

[0042] Example 3

[0043] An extraction process of safflower extract comprises the following steps:

[0044] S1, adding 10g of safflower to 100mL of an aqueous solution containing ascorbic acid and Tween 20, wherein the mass fraction of ascorbic acid in the aqueous solution is 3%, and the mass fraction of Tween 20 is 0.8%, heating and stirring at 60°C for 2h, filtering after the extraction to obtain a first filtrate and a filter residue;

[0045] S2, dispersing the filter residue in 150 mL of deionized water, and then adding composite bacteria, wherein the mass ratio of the filter residue to the composite bacteria is 100:0.5, and the mass ratio of birch tube stripping bacteria, thermophilic Streptococcus and yeast in the composite bacteria is 2.8:0.9:1.4, culturing at a constant temperature of 28°C for 7 days, then sterilizing, heating and extracting at 50°C for 2 hours, filtering, and obtaining a second filtrate;

[0046] S3. The first filtrate and the second filtrate are mixed and concentrated by rotary evaporation at a temperature of 60° C. and a pressure of 0.02 MPa. The concentration is performed until the ratio of the mass of the filtrate to the mass of safflower added in step S1 is 1:1, thereby obtaining a safflower extract.

[0047] Example 4

[0048] An extraction process of safflower extract comprises the following steps:

[0049] S1, adding 10g of safflower to 180mL of an aqueous solution containing ascorbic acid and Tween 20, wherein the mass fraction of ascorbic acid in the aqueous solution is 1.5%, and the mass fraction of Tween 20 is 1.5%, heating and stirring at 40°C for 3h, filtering after the extraction to obtain a first filtrate and a filter residue;

[0050] S2, dispersing the filter residue in 150 mL of deionized water, and then adding composite bacteria, wherein the mass ratio of the filter residue to the composite bacteria is 100:0.2, and the mass ratio of birch tube exfoliation bacteria, thermophilic Streptococcus and yeast in the composite bacteria is 2.5:0.8:1.5, culturing at a constant temperature of 28°C for 5 days, then sterilizing, heating and extracting at 50°C for 2 hours, filtering, and obtaining a second filtrate;

[0051] S3. The first filtrate and the second filtrate are mixed and concentrated by rotary evaporation at a rotary evaporation temperature of 50° C. and a pressure of 0.03 MPa. The filtrate is concentrated until the mass ratio of the filtrate to the mass of safflower added in step S1 is 1:1, thereby obtaining a safflower extract.

[0052] Comparative Example 1

[0053] An extraction process of safflower extract comprises the following steps:

[0054] S1, adding 10g of safflower to 150mL of an aqueous solution containing ascorbic acid and Tween 20, wherein the mass fraction of ascorbic acid in the aqueous solution is 2%, and the mass fraction of Tween 20 is 1%, heating and stirring at 50°C for 2.5h, filtering after the extraction to obtain a first filtrate and a filter residue;

[0055] S2, dispersing the filter residue in 150 mL of deionized water, and then adding birch tube-stripping fungi, wherein the mass ratio of the filter residue to birch tube-stripping fungi is 100:0.4, culturing at a constant temperature of 28° C. for 5 days, then sterilizing, heating and extracting at 50° C. for 2 hours, filtering, and obtaining a second filtrate;

[0056] S3. The first filtrate and the second filtrate are mixed and concentrated by rotary evaporation at a rotary evaporation temperature of 50° C. and a pressure of 0.01 MPa. The concentration is performed until the ratio of the mass of the filtrate to the mass of safflower added in step S1 is 1:1, thereby obtaining a safflower extract.

[0057] Compared with Example 1, in Comparative Example 1, only B. birchii was added.

[0058] Comparative Example 2

[0059] An extraction process of safflower extract comprises the following steps:

[0060] S1, adding 10g of safflower to 150mL of an aqueous solution containing ascorbic acid and Tween 20, wherein the mass fraction of ascorbic acid in the aqueous solution is 2%, and the mass fraction of Tween 20 is 1%, heating and stirring at 50°C for 2.5h, filtering after the extraction to obtain a first filtrate and a filter residue;

[0061] S2, dispersing the filter residue in 150 mL of deionized water, then adding thermophilic streptococci, wherein the mass ratio of the filter residue to the thermophilic streptococci is 100:0.4, culturing at a constant temperature of 28° C. for 5 days, then sterilizing, heating and extracting at 50° C. for 2 hours, filtering, and obtaining a second filtrate;

[0062] S3. The first filtrate and the second filtrate are mixed and concentrated by rotary evaporation at a rotary evaporation temperature of 50° C. and a pressure of 0.01 MPa. The concentration is performed until the ratio of the mass of the filtrate to the mass of safflower added in step S1 is 1:1, thereby obtaining a safflower extract.

[0063] Compared with Example 1, in Comparative Example 2, only Streptococcus thermophilus was added.

[0064] Comparative Example 3

[0065] An extraction process of safflower extract comprises the following steps:

[0066] S1, adding 10g of safflower to 150mL of an aqueous solution containing ascorbic acid and Tween 20, wherein the mass fraction of ascorbic acid in the aqueous solution is 2%, and the mass fraction of Tween 20 is 1%, heating and stirring at 50°C for 2.5h, filtering after the extraction to obtain a first filtrate and a filter residue;

[0067] S2, dispersing the filter residue in 150 mL of deionized water, and then adding yeast, wherein the mass ratio of the filter residue to the yeast is 100:0.4, culturing at a constant temperature of 28° C. for 5 days, then sterilizing, heating and extracting at 50° C. for 2 hours, filtering, and obtaining a second filtrate;

[0068] S3. The first filtrate and the second filtrate are mixed and concentrated by rotary evaporation at a rotary evaporation temperature of 50° C. and a pressure of 0.01 MPa. The concentration is performed until the ratio of the mass of the filtrate to the mass of safflower added in step S1 is 1:1, thereby obtaining a safflower extract.

[0069] Compared with Example 1, in Comparative Example 3, only yeast was added.

[0070] Comparative Example 4

[0071] An extraction process of safflower extract comprises the following steps:

[0072] S1, adding 10g of safflower to 150mL of an aqueous solution containing ascorbic acid and Tween 20, wherein the mass fraction of ascorbic acid in the aqueous solution is 2%, and the mass fraction of Tween 20 is 1%, heating and stirring at 50°C for 2.5h, filtering after the extraction to obtain a first filtrate and a filter residue;

[0073] S2, dispersing the filter residue in 150 mL of deionized water, and then adding composite bacteria, wherein the mass ratio of the filter residue to the composite bacteria is 100:0.4, and the mass ratio of birch tube exfoliation bacteria to thermophilic Streptococcus in the composite bacteria is 2.5:0.8, culturing at 28° C. for 5 days, then sterilizing, heating and extracting at 50° C. for 2 hours, filtering, and obtaining a second filtrate;

[0074] S3. The first filtrate and the second filtrate are mixed and concentrated by rotary evaporation at a rotary evaporation temperature of 50° C. and a pressure of 0.01 MPa. The concentration is performed until the ratio of the mass of the filtrate to the mass of safflower added in step S1 is 1:1, thereby obtaining a safflower extract.

[0075] Compared with Example 1, in Comparative Example 4, the composite bacteria consisted of Bacillus birchii and Streptococcus thermophilus.

[0076] Comparative Example 5

[0077] An extraction process of safflower extract comprises the following steps:

[0078] S1, adding 10g of safflower to 150mL of an aqueous solution containing ascorbic acid and Tween 20, wherein the mass fraction of ascorbic acid in the aqueous solution is 2%, and the mass fraction of Tween 20 is 1%, heating and stirring at 50°C for 2.5h, filtering after the extraction to obtain a first filtrate and a filter residue;

[0079] S2, dispersing the filter residue in 150 mL of deionized water, and then adding composite bacteria, wherein the mass ratio of the filter residue to the composite bacteria is 100:0.4, and the mass ratio of thermophilic streptococci to yeast in the composite bacteria is 0.8:1.2, culturing at 28° C. for 5 days, then sterilizing, heating and extracting at 50° C. for 2 hours, filtering, and obtaining a second filtrate;

[0080] S3. The first filtrate and the second filtrate are mixed and concentrated by rotary evaporation at a rotary evaporation temperature of 50° C. and a pressure of 0.01 MPa. The concentration is performed until the ratio of the mass of the filtrate to the mass of safflower added in step S1 is 1:1, thereby obtaining a safflower extract.

[0081] Compared with Example 1, in Comparative Example 5, the composite bacteria consisted of thermophilic Streptococcus and yeast.

[0082] The safflower extracts obtained in Examples 1-4 and Comparative Examples 1-5 were diluted 50 times with a 50% methanol aqueous solution by volume and then subjected to high performance liquid chromatography. The contents of hydroxysafflower yellow a and dehydrated safflower yellow b in the safflower extract were obtained according to the standard curve of the compounds (hydroxysafflower yellow a and dehydrated safflower yellow b), and the extraction rate (mg / g) was calculated, wherein the extraction rate = compound content in the extract / safflower weight,

[0083] High performance liquid chromatography (HPLC), specific detection conditions: Agilent Extend C-18 (250mm*4.6mm, 6μm); mobile phase is 0.1% formic acid water (A)-acetonitrile (B), elution program: 0-12min, 10-22%B, 12-20min, 26%B, 20-30min, 26-90%B; detection wavelength 0-7min, 270nm, 7-20min, 403nm, 20-80min, 270min; column temperature 25℃; flow rate 0.8mL / min, injection volume 20μL.

[0084] The results are shown in the following table:

[0085] Extraction rate of hydroxysafflor yellow a (mg / g) Extraction rate of dehydrated safflower yellow b (mg / g) Example 1 32.3 14.2 Example 2 31.8 13.7 Example 3 32.6 14.4 Example 4 31.5 13.8 Comparative Example 1 29.1 10.9 Comparative Example 2 28.7 10.2 Comparative Example 3 28.2 10.5 Comparative Example 4 29.8 11.4 Comparative Example 5 30.4 12.3

[0086] As can be seen from the table, compared with Example 1, in Comparative Examples 1-3 using a single strain, the extraction rates of hydroxysafflor yellow a and dehydrated safflor yellow b are not as good as those in Example 1, among which the extraction rate of Comparative Example 1 is worse than that of Comparative Example 2. The reason may be that the birch tube-exfoliating bacteria secretes more types of enzymes than thermophilic Streptococcus, and the degree of cell wall degradation is greater than that of thermophilic Streptococcus, which can promote the dissolution of more active ingredients and increase the extraction rate. It can be seen from Comparative Examples 4-5 that the present invention uses three strains of bacteria for compounding, and its extraction rate of hydroxysafflor yellow a and dehydrated safflor yellow b is higher than that of the compounding of two strains of bacteria.

[0087] Finally, it should be noted that the above embodiments do not limit the present invention in any form. For those skilled in the art, some modifications and improvements can be made to the present invention. Therefore, any modifications or improvements made without departing from the spirit of the present invention are within the scope of the present invention.

Claims

1. A process for extracting safflower extract, characterized in that: The steps include: S1, adding safflower to an aqueous solution containing ascorbic acid and Tween 20, heating and stirring to extract, and filtering after the extraction to obtain a first filtrate and a filter residue; S2, dispersing the filter residue in deionized water, then adding composite bacteria, culturing for 5-7 days, sterilizing, heating, extracting, filtering, and obtaining a second filtrate; S3. The first filtrate and the second filtrate are mixed and concentrated to obtain a safflower extract.

2. The extraction process according to claim 1, characterized in that In step S1, the usage ratio of safflower and aqueous solution is 1 g: 10-20 mL.

3. The extraction process according to claim 1, characterized in that In step S1, the mass fraction of ascorbic acid in the aqueous solution is 1-3%, and the mass fraction of Tween 20 is 0.8-1.5%.

4. The extraction process according to claim 1, characterized in that In step S1, the temperature of the heating and stirring leaching is 40-60° C., and the time of the heating and stirring leaching is 2-3 hours.

5. The extraction process according to claim 1, characterized in that In step S2, the mass ratio of the filter residue to the composite bacteria is 100:0.2-0.

5.

6. The extraction process according to claim 1, characterized in that In step S2, the composite bacteria is composed of Bacillus birchii, Streptococcus thermophilus and yeast.

7. The extraction process according to claim 6, characterized in that The mass ratio of the birch tube-exfoliating bacteria, thermophilic streptococci and yeast is 2.4-2.8:0.6-0.9:1.2-1.

5.

8. The extraction process according to claim 1, characterized in that: In step S2, the temperature of the heating extraction is 50-60°C, and the time of the heating extraction is 1-2h.

9. The safflower extract obtained by the extraction process according to any one of claims 1 to 8.

10. Use of the safflower extract according to claim 9 in preparing a drug for preventing cardiovascular and cerebrovascular diseases.