Penis cervi composition as well as preparation method and application thereof

Abstract

The invention mainly relates to a penis cervi composition as well as a preparation method and application thereof, and belongs to the technical field of biology. The composition is prepared from 50-60 parts of penis cervi, 15-25 parts of mushroom, 6-10 parts of fructus lycii, 10-20 parts of millet and 1-3 parts of sodium alginate. The penis et testis cervi composition is prepared by soaking the penis et testis cervi in honey, freeze-drying and crushing, freeze-drying and crushing Taiwan mushroom and fructus lycii respectively, parching and crushing millet, and then mixing the crushed raw materials with sodium alginate to obtain the penis et testis cervi composition. The penis cervi composition is good in taste, and can effectively resist fatigue and tonify yang.

Description

Technical Field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a deer penis composition and a preparation method and application thereof. Background Art

[0002] As people's living standards continue to improve, the pace of modern life is gradually accelerating, people's work pressure is increasing, and their dietary structure and living habits have also changed, causing people's bodies to be in a state of fatigue and their minds to be under long-term mental tension, leading to symptoms such as physical fatigue, mental fatigue, kidney deficiency and impotence, which seriously affect people's quality of life and work efficiency, and increase the incidence of chronic diseases.

[0003] The survey shows that fatigue has become one of the important factors that endanger human health, and is also one of the important causes of sub-health status and various diseases. And impotence due to kidney deficiency can also lead to a series of symptoms such as premature aging, memory loss, and abnormal emotions. At present, Western medicine usually treats impotence due to kidney deficiency by dilating penile blood vessels to improve erectile dysfunction, but there is no relevant explanation for kidney deficiency in Western medicine, because there is no concept of kidney deficiency in Western medicine, only kidney damage or renal insufficiency, and the kidney is just an organ in Western medicine. There are still no clear research results on anti-fatigue. The reason is speculated to be related to infection, immune system abnormalities, endocrine and metabolic disorders, and neuropsychiatric diseases; at the same time, social environment, psychological factors and genetic factors also have an important impact on its development. In traditional Chinese medicine, physical fatigue and kidney deficiency are considered to be a common physical state, which may be related to many factors, such as insufficient innate endowment, improper postnatal conditioning, excessive fatigue, etc., but there is still a lack of effective treatment drugs.

[0004] Deer whip is the dried penis and testicles of Cervus nippon Temminck or Cervus elaphus Linnaeus, which are Cervidae animals. It has the effects of invigorating kidney essence, strengthening kidney yang, and strengthening waist and knees, and is used to treat kidney deficiency and strain, sore waist and knees, deafness and tinnitus, impotence and spermatorrhea, and cold uterus and infertility. However, the current preparation method of deer whip, such as making wine, stewing, and high-temperature baking to prepare powder, not only has a poor taste, reduces the compliance of the user, and can not fully obtain the effective ingredients of deer whip, so that its effect cannot be fully exerted, and its single use drug effect is not good, resulting in its unsatisfactory therapeutic effect. Therefore, it is of great significance to provide a composition with good taste and anti-fatigue, kidney-invigorating and yang-strengthening effects. Summary of the invention

[0005] In view of this, the purpose of the present invention is to provide a deer penis composition and a preparation method, which can better retain the effective components of each raw material on the basis of improving the taste of taking it, so that the various raw materials in the formula can work synergistically to jointly exert the effects of anti-fatigue and aphrodisiac.

[0006] The invention provides a deer penis composition, which comprises the following raw materials in parts by weight: 50-60 parts of deer penis, 15-25 parts of Taiwan mushrooms, 6-10 parts of wolfberries, 10-20 parts of millet and 1-3 parts of sodium alginate.

[0007] Preferably, the deer penis composition comprises the following raw materials in parts by weight: 53-57 parts of deer penis, 18-22 parts of Agaricus truncatus, 7-9 parts of wolfberry, 13-17 parts of millet, and 1.5-2.5 parts of sodium alginate.

[0008] The present invention also provides a method for preparing the deer penis composition, comprising the following steps:

[0009] The deer penis is soaked in honey, freeze-dried, crushed and sieved to obtain deer penis powder;

[0010] The mushrooms are freeze-dried, crushed and sieved to obtain mushroom powder;

[0011] The millet is roasted, crushed and sieved to obtain millet flour;

[0012] Lycium barbarum is freeze-dried, crushed and sieved to obtain Lycium barbarum powder;

[0013] Deer penis powder, Taiwan mushroom powder, millet powder, wolfberry powder and sodium alginate are mixed to obtain a deer penis composite.

[0014] Preferably, the honey soaking time is 1 to 3 hours.

[0015] Preferably, freeze-drying of deer penis comprises the following steps: freezing the deer penis soaked in honey at -40°C to -50°C for 2 to 4 hours; then vacuum freezing at -45°C to -55°C for 0.5 to 2.5 hours; and then heating until the pressure reaches 28 to 32 Pa and maintaining for 6 to 8 hours.

[0016] Preferably, freeze-drying of Agaricus oleraceus and wolfberry comprises the following steps: freezing Agaricus oleraceus or wolfberry at -40℃~-50℃ for 1~3h; then vacuum freezing at -45℃~-55℃ for 0.5~1.5h; then heating to the pressure reaching 28~32Pa and maintaining for 4~6h.

[0017] Preferably, the millet is roasted at 140-160° C. for 3-5 minutes.

[0018] Preferably, the sieving is that the deer whip, Taiwan mushroom or wolfberry is sieved through a 40-70 mesh sieve, and the millet is sieved through a 40-50 mesh sieve.

[0019] Preferably, the mixing is to mix deer penis powder, Taiwan mushroom powder, millet powder, wolfberry powder and then mix with sodium alginate.

[0020] The present invention also provides application of the deer penis composition or the preparation method in preparing anti-fatigue and / or aphrodisiac products.

[0021] Beneficial effects of the present invention:

[0022] The deer whip composition of the present invention can not only enhance the efficacy of the medicine by adding millet, but also play the role of adhering to and wrapping other raw materials, thereby improving the mouthfeel of the composition. Sodium alginate has the functions of concentrating solution, forming a film, and forming a viscous and uniform liquid. After being added, it is not only conducive to forming a uniform and viscous liquid for convenient taking, but also can form a protective film on the surface of other raw material powder microparticles after encountering water, effectively preventing the effective ingredients of other raw materials from being destroyed or lost during the process of taking it with water and simmering it. At the same time, the functional ingredients of each raw material can be better retained by freeze drying. The synergistic effect of each raw material can achieve the effects of anti-fatigue and aphrodisiac together. DETAILED DESCRIPTION

[0023] The invention provides a deer penis composition, comprising the following raw materials in parts by weight: 50-60 parts of deer penis, 15-25 parts of shiitake mushrooms, 6-10 parts of wolfberries, 10-20 parts of millet, and 1-3 parts of sodium alginate; preferably: 53-57 parts of deer penis, 18-22 parts of shiitake mushrooms, 7-9 parts of wolfberries, 13-17 parts of millet, and 1.5-2.5 parts of sodium alginate; more preferably: 55 parts of deer penis, 20 parts of shiitake mushrooms, 8 parts of wolfberries, 15 parts of millet, and 2 parts of sodium alginate.

[0024] The present invention has no special limitation on the sources of the deer whip, mushroom, wolfberry, millet and sodium alginate, and conventional commercial products in the art can be used.

[0025] The present invention also provides a method for preparing the deer penis composition, comprising the following steps:

[0026] After soaking the deer penis in honey, freeze-drying, crushing and sieving to obtain deer penis powder. The honey is preferably honey with a mass concentration of 10% to 20%. In one embodiment, honey with a concentration of 10%, 15% or 20% can be selected. The honey soaking time is preferably 1 to 3 hours, more preferably 1.5 hours to 2.5 hours, and further preferably 2 hours. The freeze-drying preferably includes the following steps: freezing the deer penis soaked in honey at -40℃ to -50℃ for 2 to 4 hours; then vacuum freezing at -45℃ to -55℃ for 0.5 to 2.5 hours, and sublimation drying; then heating to a pressure of 28 to 32Pa, and maintaining for 6 to 8 hours, so as to effectively capture the sublimated vapor. In one embodiment, the freeze drying can be selected as follows: the deer penis soaked in honey is frozen at -40°C, -41°C, -42°C, -43°C, -44°C, -45°C, -46°C, -47°C, -48°C, -49°C or -50°C for 2, 2.5, 3, 3.5 or 4 hours; then vacuum frozen at -45°C, -46°C, -47°C, -48°C, -49°C, -50°C, -51°C, -52°C, -53°C, -54°C or -55°C for 0.5h, 1h, 1.5h, 2h or 2.5h; then heated to a pressure of 28Pa, 29Pa, 30Pa, 31Pa or 32Pa, and maintained for 6h, 6.5h, 7h, 7.5h or 8h. After freeze drying, it is crushed. The present invention does not specifically limit the crushing method. In one embodiment, grinding and crushing can be selected. After crushing, the powder is sieved, preferably through a 40-70 mesh sieve. In one embodiment, a 40-mesh, 50-mesh, 60-mesh or 70-mesh sieve can be selected. The large particles that do not meet the particle size requirements obtained by sieving can be preferably repeated after the above crushing and sieving steps, and the obtained powders are mixed together to obtain deer penis powder.

[0027] The mushrooms are freeze-dried, crushed and sieved to obtain mushroom powder. The freeze-drying preferably includes the following steps: freezing the mushrooms at -40℃ to -50℃ for 1 to 3 hours, then vacuum freezing at -45℃ to -55℃ for 0.5 to 1.5 hours, and sublimation drying; then heating to a pressure of 28 to 32 Pa, maintaining for 4 to 6 hours, so as to effectively capture the sublimated vapor. In one embodiment, the mushrooms can be frozen at -40°C, -41°C, -42°C, -43°C, -44°C, -45°C, -46°C, -47°C, -48°C, -49°C or -50°C for 1, 1.5, 2, 2.5 or 3 hours; then vacuum frozen at -45°C, -46°C, -47°C, -48°C, -49°C, -50°C, -51°C, -52°C, -53°C, -54°C or -55°C for 0.5h, 1h or 1.5h; then heated to a pressure of 28Pa, 29Pa, 30Pa, 31Pa or 32Pa, and maintained for 4h, 4.5h, 5h, 5.5h or 6h. After freeze drying, the mushrooms are crushed. The present invention does not specifically limit the crushing method. In one embodiment, grinding and crushing can be selected. After crushing, the powder is sieved, preferably through a 40-70 mesh sieve. In one embodiment, a 40-mesh, 50-mesh, 60-mesh or 70-mesh sieve can be selected. The large particles that do not meet the particle size requirements obtained by sieving can be preferably repeated after the above crushing and sieving steps, and the obtained powders are mixed together to obtain Taiwan mushroom powder.

[0028] The millet is roasted, crushed and sieved to obtain millet powder. The millet is preferably roasted at 140-160°C for 3-5 minutes to make the millet lightly burnt. In one embodiment, the millet can be roasted at 140°C, 145°C, 150°C, 155°C or 160°C for 3 minutes, 4 minutes or 5 minutes. After roasting, it is crushed. The present invention does not specifically limit the method of crushing. In one embodiment, grinding and crushing can be selected. After crushing, it is sieved. The sieving is preferably sieved through a 40-50 mesh sieve. In one embodiment, a 40 mesh, 45 mesh or 50 mesh sieve can be selected. The large particles that do not meet the particle size requirements obtained by sieving can be preferably repeated after the above-mentioned crushing and sieving steps, and the obtained powders are mixed together to obtain millet powder.

[0029] The wolfberry is freeze-dried, crushed and sieved to obtain wolfberry powder. The specific preparation method of the wolfberry powder is the same as the preparation method of the Taiwan mushroom powder.

[0030] Deer penis powder, Taiwan mushroom powder, millet powder, wolfberry powder and sodium alginate are mixed to obtain a deer penis composition. The mixing is preferably mixing deer penis powder, Taiwan mushroom powder, millet powder, wolfberry powder and then mixing with sodium alginate.

[0031] The present invention also provides the use of the deer penis composition or the preparation method in the preparation of anti-fatigue and / or aphrodisiac products. The deer penis composition can improve the general condition of fatigued people, increase body mass, increase endurance and physical fitness when fatigued, reduce serum lactic acid and urea nitrogen content in the body after exercise, increase liver glycogen content, and play an anti-fatigue role. The deer penis composition can also increase the weight of male accessory organs, improve mating ability, and play an aphrodisiac role.

[0032] When the deer penis composition is used, preferably a certain amount of the composition can be added to boiling water or water at room temperature and then simmered for 5 to 10 minutes, and then taken after cooling.

[0033] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0034] In the following embodiments, unless otherwise specified, all of them are conventional methods.

[0035] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0036] Example 1

[0037] Preparation of deer penis composition

[0038] Formula: 55g deer whip, 20g Taiwan mushroom, 8g wolfberry, 15g millet, and 2g sodium alginate.

[0039] Preparation method:

[0040] 1. Weigh the fresh deer penis after purification, soak it in 15% honey for 2 hours, and drain. Freeze drying: adjust the temperature of the freeze drying equipment drying box plate layer and the product to -45°C, so that the liquid component in the deer penis reaches the eutectic point, freeze drying for 3 hours; adjust the temperature to -50°C and turn on the vacuum pump, freeze drying for 1.5 hours; then slowly heat the plate layer until the pressure in the drying box reaches 30Pa, and maintain the effective pressure for 7 hours; after the moisture content is qualified (the mass fraction of moisture is ≤18%), grind it into powder, pass through a 50-mesh sieve, and repeat the above-mentioned crushing and sieving steps for the large particles that do not meet the particle size requirements, and mix the obtained powders together to obtain deer penis powder.

[0041] 2. Weigh the washed and dried mushrooms and freeze-dry them: adjust the temperature of the freeze-drying equipment drying box plate layer and the product to -45°C, so that the liquid component in the mushrooms reaches the eutectic point, and freeze-dry for 2 hours; then adjust the temperature to -50°C and turn on the vacuum pump, freeze-dry for 1 hour; then slowly heat the plate layer until the pressure in the drying box reaches 30Pa, and maintain the effective pressure for 5 hours; after the moisture content is qualified (the mass fraction of moisture is ≤18%), grind it into powder, pass through a 50-mesh sieve, and repeat the above-mentioned crushing and sieving steps for the large particles that do not meet the particle size requirements, and mix the obtained powders together to obtain mushroom powder.

[0042] 3. Weigh millet, fry it in a drum frying pan at 150° C. for 3 min, crush it into powder, pass it through a 45-mesh sieve, and repeat the above crushing and sieving steps for the large particles that do not meet the particle size requirements. Then, mix the obtained powders together to obtain millet flour.

[0043] 4. Weigh the washed and dried wolfberries, freeze-dry them (the specific steps are the same as 2. of the present embodiment), grind them into powder after the freeze-dried moisture content is qualified (the mass fraction of moisture is ≤18%), pass through a 50-mesh sieve, and sieve the large particles that do not meet the particle size requirements. After repeating the above-mentioned crushing and sieving steps, the obtained powders are mixed together to obtain wolfberry powder.

[0044] 5. Mix the deer penis powder, Taiwan mushroom powder, millet powder and wolfberry powder together and stir evenly.

[0045] 6. Add sodium alginate and stir evenly again to obtain a deer penis composition.

[0046] Example 2

[0047] Preparation of deer penis composition

[0048] Formula: 50g deer whip, 25g Taiwan mushroom, 6g wolfberry, 20g millet, and 3g sodium alginate.

[0049] Preparation method:

[0050] 1. Weigh the fresh deer penis after purification, soak it in 10% honey for 1 hour, and drain. Freeze drying: adjust the temperature of the freeze drying equipment drying box plate layer and the product to -40°C, so that the liquid component in the deer penis reaches the eutectic point, and freeze dry for 4 hours; adjust the temperature to -45°C and turn on the vacuum pump, freeze dry for 2.5 hours; then slowly heat the plate layer until the pressure in the drying box reaches 28Pa, and maintain the effective pressure for 8 hours; after the moisture content is determined to be qualified (the mass fraction of moisture is ≤18%), grind it into powder, pass through a 40-mesh sieve, and repeat the above-mentioned crushing and sieving steps for the large particles that do not meet the particle size requirements, and mix the obtained powders together to obtain deer penis powder.

[0051] 2. Weigh the washed and dried mushrooms and freeze-dry them: adjust the temperature of the freeze-drying equipment drying box plate layer and the product to -40°C, so that the liquid component in the mushrooms reaches the eutectic point, and freeze-dry for 3 hours; then adjust the temperature to -45°C and turn on the vacuum pump, freeze-dry for 1.5 hours; then slowly heat the plate layer until the pressure in the drying box reaches 28Pa, and maintain the effective pressure for 6 hours; after the moisture content is qualified (the mass fraction of moisture is ≤18%), grind it into powder, pass through a 40-mesh sieve, and repeat the above-mentioned crushing and sieving steps for the large particles that do not meet the particle size requirements, and mix the obtained powders together to obtain mushroom powder.

[0052] 3. Weigh the millet, fry it in a drum frying pan at 140°C for 4 minutes, crush it into powder, pass it through a 40-mesh sieve, and repeat the above crushing and sieving steps for the large particles that do not meet the particle size requirements. Then, mix the obtained powders together to obtain millet flour.

[0053] 4. Weigh the washed and dried wolfberries, freeze-dry them (the specific steps are the same as 2. of the present embodiment), grind them into powder after the moisture content is qualified (the mass fraction of moisture is ≤18%) after freeze-drying, pass through a 40-mesh sieve, and sieve the large particles that do not meet the particle size requirements. After repeating the above-mentioned crushing and sieving steps, the obtained powders are mixed together to obtain wolfberry powder.

[0054] 5. Mix the deer penis powder, Taiwan mushroom powder, millet powder and wolfberry powder together and stir evenly.

[0055] 6. Add sodium alginate and stir evenly again to obtain a deer penis composition.

[0056] Example 3

[0057] Preparation of deer penis composition

[0058] Formula: Deer whip 60g, Taiwan mushroom 15g, wolfberry 10g, millet 10g, sodium alginate 1g.

[0059] Preparation method:

[0060] 1. Weigh the fresh deer penis after purification, soak it in 20% honey for 3 hours, and drain. Freeze drying: adjust the temperature of the freeze drying equipment drying box plate layer and the product to -50°C, so that the liquid component in the deer penis reaches the eutectic point, and freeze dry for 2 hours; adjust the temperature to -55°C and turn on the vacuum pump, freeze dry for 0.5 hours; then slowly heat the plate layer until the pressure in the drying box reaches 32Pa, and maintain the effective pressure for 6 hours; after the moisture content is qualified (the mass fraction of moisture is ≤18%), grind it into powder, pass through a 70-mesh sieve, and repeat the above-mentioned crushing and sieving steps for the large particles that do not meet the particle size requirements, and mix the obtained powders together to obtain deer penis powder.

[0061] 2. Weigh the washed and dried mushrooms and freeze-dry them: adjust the temperature of the freeze-drying equipment drying box plate layer and the product to -50°C, so that the liquid component in the mushrooms reaches the eutectic point, and freeze-dry for 1 hour; then adjust the temperature to -55°C and turn on the vacuum pump, freeze-dry for 0.5 hour; then slowly heat the plate layer until the pressure in the drying box reaches 32Pa, and maintain the effective pressure for 4 hours; after the moisture content is qualified (the mass fraction of moisture is ≤18%), grind it into powder, pass through a 70-mesh sieve, and repeat the above-mentioned crushing and sieving steps for the large particles that do not meet the particle size requirements, and mix the obtained powders together to obtain mushroom powder.

[0062] 3. Weigh millet, fry it in a drum frying pan at 160°C for 5 minutes, crush it into powder, pass it through a 50-mesh sieve, and repeat the above crushing and sieving steps for large particles that do not meet the particle size requirements. Then, mix the obtained powders together to obtain millet flour.

[0063] 4. Weigh the washed and dried wolfberries, freeze-dry them (the specific steps are the same as 2. of the present embodiment), grind them into powder after the moisture content is determined to be qualified (the mass fraction of moisture is ≤18%) after freeze-drying, and pass through a 70-mesh sieve. After sieving the large particles that do not meet the particle size requirements, repeat the above-mentioned crushing and sieving steps, and mix the obtained powders together to obtain wolfberry powder.

[0064] 5. Mix the deer penis powder, Taiwan mushroom powder, millet powder and wolfberry powder together and stir evenly.

[0065] 6. Add sodium alginate and stir evenly again to obtain a deer penis composition.

[0066] Comparative Example 1

[0067] The only difference from Example 1 is that ① millet is removed from the formula, and ② in the preparation method: step "3." The preparation process of millet powder is removed; step 5 is "mixing deer penis powder, Taiwan mushroom powder, and wolfberry powder together and stirring evenly." The rest is the same as Example 1. The prepared composition is recorded as a comparative composition.

[0068] Comparative Example 2

[0069] Take 50 g of deer penis and prepare deer penis powder according to step 1 of Example 1.

[0070] Test Example 1

[0071] Taste improvement research

[0072] 1 Materials: The test materials are the deer penis composition prepared in Example 1 and the comparative composition prepared in Comparative Example 1, and the detection instrument is the TS-5000Z taste analysis system (INSENT, Japan).

[0073] 2 Methods and Results

[0074] 2.1 Analysis of taste properties of electronic tongue

[0075] 3 g of each sample of the deer penis composition and the comparative composition was taken, added with 100 mL of purified water, and fully dissolved by ultrasonication (300 W, 40 kHz) for 5 min, and filtered. -1 Potassium chloride + 0.3mmol·L -1 Tartaric acid) was used as a control, and the filtrate was analyzed by TS-5000Z taste analysis system for AHS (sourness), CTS (saltiness), NMS (umami), ANS (sweetness), and SCS (bitterness). Each sample was measured three times.

[0076] The taste test data are all absolute output values ​​based on artificial saliva (reference solution). The state of artificial saliva in the electronic tongue test simulates the state when there is only saliva in the human mouth. The results are shown in Table 1. The sourness and saltiness of the two groups of samples are below the tasteless point. In addition, the other taste indicators are above the tasteless point. The unpleasant taste is mainly bitter. Through analysis, it is found that sweetness accounts for a larger proportion of the taste, and the deer penis combination has a better taste.

[0077] Table 1 Electronic tongue taste analysis

[0078] sample Sweetness Salty sour bitterness Umami Richness Bad taste area Artificial saliva (reference solution) 0 -6 -13 0 0 0 Comparative composition 3.75 -7.8 -23.4 6.9 1.45 1.37 22.43 Deer penis composition 6.84 -7.2 -22.5 3.46 6.82 2.87 3.92

[0079] Note: Tasteless (reference solution output) is the tasteless point, so the tasteless point of sour taste is -13, and the tasteless point of salty taste is -6. Based on this, when the taste value of the sample is lower than Tasteless, it means that the sample has no such taste, otherwise it has.

[0080] 2.2 Artificial Taste Sweetness Analysis

[0081] 2.2.1 Volunteer screening: 18 volunteers were randomly selected from 30 volunteers as subjects (22-35 years old, without severe allergies, genetic disease history, cholecystitis, bad habits, and recent disease history, including 6 males and 12 females) to conduct oral tasting experiments on the samples.

[0082] 2.2.2 Oral tasting method: 10g of the comparative composition was added with boiled water to prepare a 30mL solution, and 10g of the deer penis composition was added with boiled water to prepare a 30mL solution. The two solutions were placed in disposable plastic cups respectively, and the subjects held them in their mouths for 15 seconds. During this time, the mouth was rinsed to allow the taste perception area at the root and side of the tongue to feel the taste of the medicine. After 15 seconds, it was spit out, and a pause of 60 seconds was made to feel the aftertaste. The mouth was rinsed 5 times, and a rest of 30 minutes was taken between each round of samples. The experiment adopted a random single-blind method to test the samples of the subjects.

[0083] 2.2.3 Evaluation criteria: Sweetness is divided into 5 levels, and each level is assigned a certain sweetness value range, see Table 2.

[0084] Table 2 Qualitative description, grade and quantitative range of sweetness value

[0085] Serial number Taste description of sweetness Sweetness level Sweetness value range 1 Almost no sweetness Ⅰ [0.5,1.5) 2 Slightly sweet Ⅱ [1.5,2.5) 3 Acceptable sweetness Ⅲ [2.5,3.5) 4 Very sweet, but still tolerable Ⅳ [3.5,4.5) 5 Unbearable sweetness Ⅴ [4.5,5.5)

[0086] The sweetness scores of 18 subjects showed that the sweetness score of the control composition was 0.76±0.16, and the sweetness score of the deer penis composition was 1.42±0.35. The overall difference between the two samples was statistically significant, and the deer penis composition tasted better.

[0087] The above results show that the addition of millet significantly improves the overall taste of the composition.

[0088] Test Example 2

[0089] Anti-fatigue research

[0090] According to the "Test Methods for the Function of Health Foods in Relieving Physical Fatigue 2020", a positive result of weighted swimming and two of the three biochemical indicators of serum lactate, serum urea nitrogen, and liver glycogen / muscle glycogen are positive can prove that the test sample has anti-fatigue effects.

[0091] 1 Materials and methods

[0092] 1.1 Materials: the deer penis composition prepared in Example 1 and the deer penis powder prepared in Comparative Example 2, dexamethasone, a blood ureanitrogen (BUN) test kit, a lactic acid (LD) test kit, and a glycogen test kit.

[0093] 1.2 Grouping and modeling methods

[0094] 40 male Kunming mice were randomly divided into 4 groups according to body weight, with 10 mice in each group. That is, blank group, model group, deer whip composition group, and deer whip powder group. After 7 days of adaptive feeding, except for the blank group, the remaining groups were gavaged with dexamethasone solution (0.35 mg / kg) every afternoon to prepare the model, and the blank group was gavaged with an equal amount of distilled water. The modeling was continued for 14 days, and no deaths or the like occurred in each group of mice during the modeling period. At the same time as the modeling, the deer whip composition group was gavaged with the deer whip composition prepared in Example 1, and the deer whip powder group was gavaged with the deer whip powder prepared in Comparative Example 2, once a day, for 14 consecutive days, and the gavage dose was 1.8 g / kg·d (human equivalent dose 9 g / kg·d). The exhaustive swimming experiment was started 30 minutes after administration on the 14th day, and the exhaustive swimming without weight bearing was performed on the 15th day, and the rest was 30 minutes, and the material was collected.

[0095] 1.3 Experiment

[0096] 1.3.1 Effects of the deer penis composition on the general condition and body weight of mice

[0097] The general condition of the mice was observed during the experiment, and the body weight of the mice was measured on the 14th day of administration.

[0098] General condition results: Compared with the blank group, the mice in the model group showed symptoms such as increased water drinking, yellow urine, increased autonomous activity, and irritability; compared with the model group, the deer penis combination group and the deer penis powder group were able to improve the condition of the mice to a certain extent.

[0099] Results of mouse body weight: Compared with the blank group, the model group had a significant decrease in body weight (P<0.05), compared with the model group, the deer penis powder group had a significant increase in body weight (P<0.05), and the deer penis combination group had a significant increase in body weight (P<0.05). See Table 3.

[0100] Table 3 Effect of deer penis composition on body weight of mice

[0101] Group Body mass (g) Blank Group 37.15±3.24 Model Group 31.34±1.24* Deer penis powder group 35.23±2.78# Deer penis combination 35.87±1.45#

[0102] Note: * indicates significant difference compared with the blank group, P<0.05; # indicates significant difference compared with the model group, P<0.05.

[0103] 1.3.2 Effect of deer penis combination on exhaustive swimming time of mice

[0104] On the 14th day, 30 minutes after drug administration, a weight-bearing swimming test was performed. The mice swam in a 75cm×55cm×40cm swimming box. The mice were artificially driven to swim continuously. The water temperature was maintained at (25±1.0)℃. The weight of the mice was a lead block of 5% of their body weight. The time from the start of swimming to the time of being completely immersed in water for 5 to 8 seconds without surfacing was recorded as the exhaustive swimming time.

[0105] Results: Compared with the blank group, the exhaustive swimming time of mice in the model group was significantly decreased (P<0.05); compared with the model group, the exhaustive swimming time of mice in the deer penis powder group and the deer penis combination group was significantly increased (P<0.05), and the effect of the deer penis combination group was significantly better than that of the deer penis powder group, as shown in Table 4. This shows that the deer penis combination of the present invention can increase endurance and physical fitness during fatigue and play an anti-fatigue role.

[0106] Table 4 Effect of deer penis composition on exhaustive swimming time of mice

[0107] Group Swimming to exhaustion (seconds) Blank Group 485.7±123.24 Model Group 231.4±35.2* Deer penis powder group 435.4±122.8# Deer penis combination 535.8±141.45#

[0108] Note: * indicates significant difference compared with the blank group, P<0.05; # indicates significant difference compared with the model group, P<0.05.

[0109] 1.3.3 Effects of Deer Penis Composition on Lactic Acid, Urea Nitrogen and Glycogen in Mice

[0110] On the morning of the 15th day of the experiment, the mice were subjected to exhaustive swimming exercise without weight bearing. After 30 minutes, the eyeballs of the mice were removed and blood was collected to kill them. After standing for 4 hours, the mice were centrifuged at 3000 r / min for 15 minutes to collect serum. Serum lactate, urea nitrogen and glycogen were tested according to the instructions of the kit.

[0111] Results: Compared with the blank group, the serum lactic acid and urea nitrogen levels of the mice in the model group were significantly increased (P<0.05), and the glycogen content of the mice in the model group was significantly decreased (P<0.05); compared with the model group, the serum lactic acid and urea nitrogen levels of the mice in the deer penis combination group decreased to varying degrees (P<0.05), and the liver glycogen content of the mice was significantly increased (P<0.05), and the effect of the deer penis combination was better than that of deer penis powder, indicating that the deer penis combination can reduce the serum lactic acid and urea nitrogen levels of mice after exercise, increase the liver glycogen content, and play a role in delaying the occurrence of fatigue or enhancing anti-fatigue, as shown in Table 5.

[0112] Table 5 Effect of deer penis composition on metabolic substances in mice

[0113]

[0114]

[0115] Note: * indicates significant difference compared with the blank group, P<0.05; # indicates significant difference compared with the model group, P<0.05.

[0116] Test Example 3

[0117] Aphrodisiac Effect Research

[0118] 1 Materials: the deer penis composition prepared in Example 1, testosterone.

[0119] 2. Test methods and results

[0120] 2.1 Effects on the accessory organs of castrated rats

[0121] Thirty SD male rats, weighing 70-90g, underwent bilateral orchiectomy under aseptic conditions with ether anesthesia. After 3 days of routine anti-inflammatory treatment with penicillin, they were randomly divided into 3 groups, 10 rats in each group, namely castration + distilled water group, castration + testosterone group, and castration + deer penis combination group. The testosterone dosage was 6mg / kg, and the deer penis combination dosage was 1g / kg. The administration began 3 days after the routine anti-inflammatory treatment with penicillin, once a day, for 15 consecutive days. The rats were killed 30 minutes after the last administration, and the preputial glands, seminal vesicles and prostate were removed to measure the organ coefficient.

[0122] The results of statistical processing are shown in Table 6. As shown in Table 6, the deer penis composition has a significant weight-increasing effect on the accessory organs of castrated male rats (P<0.05).

[0123] Table 6 Effect of deer penis composition on the accessory organs of castrated male rats

[0124] Group Preputial gland (mg / 100g) Sperm sac + prostate (mg / 100g) Castration + distilled water group 65.1±13.4 35.8±4.34 Castration + testosterone group 145.4±20.8* 1254.35±103.4* Castration + deer penis combination group 80.4±10.5* 58.3±6.4*

[0125] Note: * indicates significant difference compared with castration + distilled water group, P<0.05

[0126] 2.2 Mating test

[0127] Thirty SD male rats, weighing 180-220g, underwent bilateral orchiectomy under aseptic conditions with ether anesthesia. After 3 days of routine anti-inflammatory treatment with penicillin, they were randomly divided into 3 groups, 10 rats in each group, namely castration + distilled water group, castration + testosterone group, and castration + deer penis combination group. The testosterone dosage was 6mg / kg, and the deer penis combination dosage was 1g / kg. The administration began 3 days after routine anti-inflammatory treatment with penicillin after surgery, once a day, for 30 consecutive days. 30 minutes after the last administration, the male rats were placed alone in a 42cm×26cm×18cm cage. After 5 minutes of adaptation, a female rat was placed in it, and the latency period (first capture time) of the male rat catching the female rat and the number of captures within 20 minutes were observed and recorded.

[0128] The results are shown in Table 7. When the deer penis composition was continuously administered to rats for 30 days, its mating ability was significantly improved, the capture latency was significantly shortened (P<0.05), and the capture times were significantly increased (P<0.05).

[0129] Table 7 Effect of deer penis composition on the mating ability of castrated male rats

[0130] Group Capture latency (minutes) Number of captures (times / 20min) Castration + distilled water group 15.1±3.4 0.8±0.34 Castration + testosterone group 5.4±0.8* 14.35±3.4* Castration + deer penis combination group 8.4±0.5* 4.3±0.4*

[0131] Note: * indicates significant difference compared with castration + distilled water group, P<0.05.

[0132] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A deer penis composition, characterized in that: The invention comprises the following raw materials in parts by weight: 50-60 parts of deer whip, 15-25 parts of Taiwan mushroom, 6-10 parts of wolfberry, 10-20 parts of millet and 1-3 parts of sodium alginate.

2. The deer penis composition according to claim 1, characterized in that The invention comprises the following raw materials in parts by weight: 53-57 parts of deer whip, 18-22 parts of Taiwan mushroom, 7-9 parts of wolfberry, 13-17 parts of millet and 1.5-2.5 parts of sodium alginate.

3. The method for preparing the deer penis composition according to any one of claims 1 to 2, characterized in that: The following steps are involved: The deer penis is soaked in honey, freeze-dried, crushed and sieved to obtain deer penis powder; The mushrooms are freeze-dried, crushed and sieved to obtain mushroom powder; The millet is roasted, crushed and sieved to obtain millet flour; Lycium barbarum is freeze-dried, crushed and sieved to obtain Lycium barbarum powder; Deer penis powder, Taiwan mushroom powder, millet powder, wolfberry powder and sodium alginate are mixed to obtain a deer penis composite.

4. The preparation method according to claim 3, characterized in that: The honey soaking time is 1 to 3 hours.

5. The preparation method according to claim 3, characterized in that: The freeze-drying of deer penis comprises the following steps: freezing the deer penis soaked in honey at -40℃ to -50℃ for 2 to 4 hours; then vacuum freezing at -45℃ to -55℃ for 0.5 to 2.5 hours; and then heating until the pressure reaches 28 to 32Pa and maintaining for 6 to 8 hours.

6. The preparation method according to claim 3, characterized in that: The freeze drying of Agaricus oleraceus and wolfberry comprises the following steps: freezing Agaricus oleraceus or wolfberry at -40-50℃ for 1-3h; then vacuum freezing at -45-55℃ for 0.5-1.5h; and then heating until the pressure reaches 28-32Pa and maintaining for 4-6h.

7. The preparation method according to claim 3, characterized in that: The millet is roasted at 140-160° C. for 3-5 minutes.

8. The preparation method according to claim 3, characterized in that: The sieving is that the deer whip, Taiwan mushroom or wolfberry is sieved through a 40-70 mesh sieve, and the millet is sieved through a 40-50 mesh sieve.

9. The preparation method according to claim 3, characterized in that: The mixing is to mix deer penis powder, Taiwan mushroom powder, millet powder and wolfberry powder and then mix with sodium alginate.

10. Use of the deer penis composition according to any one of claims 1 to 2 or the preparation method according to any one of claims 3 to 9 in the preparation of anti-fatigue and / or aphrodisiac products.

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