Device and method applied to anaerobic ammonium oxidation strain enrichment culture

Abstract

The invention relates to the technical field of high-concentration ammonia nitrogen wastewater treatment, and discloses a device and a method applied to anaerobic ammonia oxidation strain enrichment culture, the device comprises a biological reaction tank, a heater, an online monitoring probe and a filter element arranged in the biological reaction tank, the on-line monitoring probe is used for monitoring the pH value, the temperature value and the dissolved oxygen content in the biological reaction tank; the heater is used for heating the biological reaction tank, the biological reaction tank is provided with a water inlet and a water outlet, the filter element is used for enriching anaerobic ammonia oxidation granular sludge in the biological reaction tank, and a liquid outlet of the filter element is communicated with the water outlet. According to the scheme, bacterial sludge is enriched through the filter element, and the retention time of the bacterial sludge is furthest realized, so that the deamination treatment load of the device is improved; the device has the advantages of high effective volume utilization rate and stable effluent quality, and is widely applicable to denitrification treatment of industrial wastewater with high ammonia nitrogen, low carbon nitrogen ratio and high suspended solids.

Description

Technical Field

[0001] The present invention relates to the technical field of high-concentration ammonia nitrogen wastewater treatment, and in particular to a device and method for enriching and culturing anaerobic ammonia-oxidizing bacteria. Background Art

[0002] High-concentration ammonia nitrogen wastewater refers to wastewater with a high concentration of ammonia nitrogen (NH3-N), usually from agriculture, chemical, pharmaceutical, food processing and other industries. If this type of wastewater is not effectively treated, it will have a serious impact on the environment, especially eutrophication and water pollution. Although biological denitrification technology is more common than physical and chemical denitrification technology, it has the characteristics of mild reaction conditions, low treatment cost and good denitrification effect, and has always been the most widely used denitrification method in the field of sewage treatment. However, for wastewater with high ammonia nitrogen and low C / N, the severe lack of organic carbon sources is a barrier to improving its denitrification efficiency, and the addition of external carbon sources will greatly increase the cost of sewage denitrification.

[0003] Anaerobic ammonium oxidation is a new biological denitrification technology. Under anaerobic or anoxic conditions, anaerobic ammonium oxidizing bacteria (AnAOB) use NO 2－ -N is the electron acceptor, with NH 4+ -N is an electron donor, converting NH 4+ -N and NO 2－ -N is converted into N2. Compared with traditional nitrification and denitrification treatment technology, anaerobic ammonium oxidation technology has the advantages of no need for external carbon source, small footprint, less sludge, high denitrification load, low oxygen supply, and low operating costs. At present, anaerobic ammonium oxidizing bacteria are cultured in an upflow anaerobic sludge blanket (UASB) reactor. In actual use, there are problems such as granular sludge loss, local dead zones, and sludge floating. Therefore, it is very necessary to provide a device and method for enriching and culturing anaerobic ammonium oxidizing bacteria. Summary of the invention

[0004] The present invention aims to provide a device and method for enriching and culturing anaerobic ammonia-oxidizing bacteria, so as to solve the problem of low treatment efficiency when the current biological denitrification technology is applied to wastewater with high ammonia nitrogen and low carbon-nitrogen ratio.

[0005] To achieve the above-mentioned purpose, the present invention provides a device for enriching and culturing anaerobic ammonia-oxidizing bacteria, which specifically includes: a biological reaction tank, a heater, an online monitoring probe, and a filter element arranged in the biological reaction tank, wherein the online monitoring probe is used to monitor the pH value, temperature value and dissolved oxygen content in the biological reaction tank; the heater is used to heat the biological reaction tank, the biological reaction tank is provided with a water inlet and a water outlet, the filter element is used for enriching the anaerobic ammonia-oxidizing granular sludge in the biological reaction tank, and the discharge port of the filter element is connected to the water outlet.

[0006] In actual applications, the inventors have found that due to the long period of anaerobic ammonium oxidizing bacteria aggregation to form biofilms, and the failure of biofilm formation due to unfavorable factors, even through conventional inoculation reinforcement or ectopic culture, the formation of anaerobic ammonium oxidizing biofilms and the start-up of the process still require several months or even longer, and the slow doubling time of anaerobic ammonium oxidizing bacteria greatly increases the difficulty of engineering application of biological denitrification technology; and anaerobic ammonium oxidizing bacteria have poor retention capacity in actual denitrification processes and are easy to lose, further restricting the application of biological denitrification technology. Therefore, the inventors developed the above-mentioned device to achieve rapid and efficient enrichment of anaerobic ammonium oxidizing bacteria, achieve rapid start-up of the anaerobic ammonium oxidizing process, and improve the system's load tolerance potential.

[0007] Preferably, a partition is provided in the bioreactor, which divides the bioreactor into a plurality of cells, and a reaction channel is formed between the partition and the bioreactor. The partition prolongs the flow path of the wastewater in the bioreactor, increases the reaction time between the wastewater and the microorganisms, is beneficial to the reproduction of the microorganisms, and improves the treatment effect of high-concentration ammonia nitrogen wastewater.

[0008] Preferably, it also includes a stirring blade and a stirring motor for driving the stirring blade to rotate, wherein the stirring motor and the stirring blade are used to stir and disturb the dead zone of the biological reaction tank. In order to avoid the dead zone formed by the local fluidity of the biological reaction tank being too small, the stirring blade is set to stir this part of the area, so that the sludge and microorganisms are mixed better, and the treatment effect of high-concentration ammonia nitrogen wastewater is improved.

[0009] Preferably, the water inlet is located at the bottom of the bioreactor, and the water outlet is located at the top of the bioreactor. If the water inlet is set at the bottom of the bioreactor, the sludge will be precipitated accordingly under the action of gravity, and the sludge in the bioreactor will be more easily enriched; if the water outlet is set at the top of the bioreactor, the sludge and water will be separated under the action of gravity, so that more water is discharged from the water outlet, reducing the loss of sludge in the bioreactor.

[0010] Preferably, a stuffing box is provided in the biological reaction tank, and a stuffing wire and a stuffing are provided in the stuffing box, and the stuffing is fixed on the stuffing wire. Microorganisms adhere to the stuffing box for reproduction and growth, and the stuffing box increases the contact area between the microorganisms and the wastewater, which is beneficial to the reproduction of microorganisms and improves the treatment effect of high-concentration ammonia nitrogen wastewater.

[0011] Preferably, the bioreactor further comprises an aeration pump and an aeration plate, wherein the aeration pump and the aeration plate are connected and the aeration plate is located at the bottom of the bioreactor. The bioreactor is aerated by the aeration pump and the aeration plate to adjust the oxygen concentration in the bioreactor.

[0012] Preferably, the filter element comprises a disc-shaped hollow ceramic membrane and a hollow rotating shaft, the hollow rotating shaft is rotatably arranged on the bioreactor, the disc-shaped hollow ceramic membrane is sleeved on the hollow rotating shaft in parallel with each other, the surface of the hollow rotating shaft is provided with a through hole connected to the disc-shaped hollow ceramic membrane, one end of the hollow rotating shaft is closed, and the other end of the hollow rotating shaft is provided with a drain, and the drain is connected to the water outlet of the bioreactor. Wastewater in the bioreactor enters the filter channel inside the disc-shaped hollow ceramic membrane from the surface thereof, and under the action of the hollow ceramic of the disc, large particles gradually adhere to the disc-shaped hollow ceramic membrane, and the liquid enters the hollow rotating shaft from the filter channel of the disc-shaped hollow ceramic membrane, and then is discharged from the water outlet of the bioreactor through the hollow rotating shaft. The anaerobic ammonia oxidation granular sludge is effectively intercepted by the disc-shaped hollow ceramic membrane, the loss of anaerobic ammonia oxidation bacteria is avoided to the greatest extent, and a high concentration of stable performance and rich population of microbial flora is formed in the reactor to ensure the denitrification efficiency.

[0013] Preferably, a backwashing assembly for backwashing the disc-shaped hollow ceramic membrane is further included, and the backwashing assembly is connected to the drain port of the hollow rotating shaft. The disc-shaped hollow ceramic membrane is backwashed regularly through the backwashing assembly at the drain port end of the hollow rotating shaft to prevent the formation of a thick filter cake layer that causes the membrane flux to be further reduced.

[0014] Preferably, it also includes a return sludge pipe and a sludge return pump, which are arranged on the return sludge pipe and are used to pump the sludge enriched by the disc-shaped hollow ceramic membrane at the outlet end to the water inlet end. The sludge is returned to increase the concentration of microorganisms at the water inlet end of the biological reaction tank and improve the treatment effect of high-concentration ammonia nitrogen wastewater.

[0015] In order to achieve the above object, the present invention also provides a method for enriching and culturing anaerobic ammonia-oxidizing bacteria, comprising the following steps:

[0016] Step 1: Inoculate sludge to start the bioreactor: Inoculate the bioreactor with activated sludge from a municipal sewage plant or sludge with anaerobic ammonium oxidation activity to make the sludge concentration in the bioreactor reach 4500-5000 mg / L, use a continuous flow mode to transport the leachate from the garbage incineration plant into the bioreactor, the effluent flow rate of the bioreactor is consistent with the inlet flow rate, start the stirring motor and stirring blades to stir the inside of the bioreactor, heat the bioreactor, the heating temperature is 30-35°C, and the stirring blade speed is 100-200rpm / min; the filter element enriches the anaerobic ammonium oxidation granular sludge in the bioreactor;

[0017] Step 2: Enrichment and cultivation of anaerobic ammonium oxidizing microorganisms: Continuously monitor the nitrite nitrogen concentration in the effluent of the bioreactor, and adjust the residence time of the wastewater in the bioreactor according to the nitrite nitrogen concentration in the effluent to accelerate the granulation of anaerobic ammonium oxidizing bacteria; and control the dissolved oxygen in the bioreactor to 0.4-0.5 mg / L, and the ammonia nitrogen and total nitrogen removal load to 0.1 kg / (m 3 ·d), and the effluent nitrite nitrogen concentration is lower than 20 mg / L, the sludge acclimation culture is completed;

[0018] Step 3: Anaerobic ammonium oxidizing microorganisms continue to reproduce stably in the treatment of high ammonia nitrogen wastewater: The anaerobic ammonium oxidizing microorganisms attached to the filler of the biological reactor continue to reproduce in the treatment of high ammonia nitrogen wastewater.

[0019] Aiming at the shortcomings of the existing upflow anaerobic sludge blanket (UASB) technology, such as granular sludge loss, local dead zone and sludge floating, the present invention introduces a rotating ceramic membrane into the reactor to form granular sludge in the reactor through sludge taming, and reduces the NH in the influent. 4+ and NO 2- or NO 3- The effect of simultaneous removal of ammonia and nitrate nitrogen is achieved through the action of anaerobic ammonia-oxidizing bacteria.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] (1) The cross-flow filtration is generated by the rotation of the ceramic membrane to achieve the enrichment of the anaerobic ammonium oxidation granular sludge in the biological reactor, which can minimize the loss of anaerobic ammonium oxidation bacteria; at the same time, the continuous inflow and outflow of water in the reactor is achieved, and no sedimentation tank is required in the process.

[0022] (2) Ceramic membrane components are used to replace the structure of traditional three-phase separators such as anaerobic reactors (such as UASB). The effective volume utilization rate is high, and there is no dead zone when the sludge floats up. A high-concentration microbial flora with stable performance and rich population is formed in the reactor to ensure the denitrification efficiency.

[0023] (3) The membrane used in the present invention is a ceramic membrane with a smooth surface and high-speed rotation, thereby increasing the flow rate of the fluid inside the membrane assembly and increasing the shear force of the fluid, which can not only more effectively reduce the deposition of pollutants on the membrane surface under the suction action of the outlet pump, but also peel off pollutants that are not tightly adsorbed on the membrane surface, effectively reducing the pollution of the ceramic membrane assembly. This device has significant tolerance to water inlet indicators such as suspended matter and organic matter, and the suspended solids concentration of the reactor outlet water is greatly reduced.

[0024] The device and process of the present application couples high-efficiency biological denitrification while effectively improving the poor retention capacity of anaerobic ammonia-oxidizing bacteria in the denitrification process. Through the granulated sludge in which aerobic ammonia-oxidizing bacteria (AerAOB), anaerobic ammonia-oxidizing bacteria (AnAOB) and denitrifying bacteria (DNB) coexist, denitrifying functional granular sludge can be quickly cultivated, effectively improving the treatment efficiency of high ammonia-nitrogen and low carbon-nitrogen ratio wastewater. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 A schematic cross-sectional view of the structure of a device for enriching and culturing anaerobic ammonia-oxidizing bacteria according to an embodiment of the present invention is shown.

[0026] Figure 2 A schematic longitudinal cross-sectional view of the structure of a device for enriching and culturing anaerobic ammonia-oxidizing bacteria according to an embodiment of the present invention is shown.

[0027] Figure 3 A schematic top view of the structure of a device for enriching and culturing anaerobic ammonia-oxidizing bacteria according to an embodiment of the present invention is shown.

[0028] Figure 4 A schematic diagram of the structure of a filler box used in an anaerobic ammonia-oxidizing bacteria enrichment and culture device according to an embodiment of the present invention is shown. DETAILED DESCRIPTION

[0029] The following is further described in detail through specific implementation methods:

[0030] The figure marks in the drawings of the specification include: biological reaction tank 1, stirring motor 2, stirring blade 3, water inlet pipe 4, water inlet pump 5, water inlet valve 6, stuffing box 7, online monitoring probe 8, partition 9, blower 10, air volume regulating valve 11, aeration disk 12, hollow rotating shaft 13, tank body insulation sleeve 14, filter element 15, return sludge pipe 16, sludge return valve 17, sludge return pump 18, aeration pipe 19, aeration flowmeter 20, rotating motor 21, disc-shaped hollow ceramic membrane 22, water outlet flowmeter 23, water outlet pipe 24, water outlet valve 25, backflush valve 26, backflush pump 27, pressure gauge 28, water pump 29, drain pipe 30, water storage tank 31, stuffing hanging wire 32, stuffing 33.

[0031] Example:

[0032] A device for enriching and culturing anaerobic ammonia-oxidizing bacteria, such as Figure 1 - Attachment Figure 4 As shown, it includes: a bioreactor 1, a heater, a filter element 15 and an online monitoring probe 8 arranged in the bioreactor 1.

[0033] The online monitoring probe 8 is used to monitor the pH value, temperature value and dissolved oxygen content in the bioreactor. It can timely obtain the dissolved oxygen concentration, temperature and pH value in the bioreactor 1, and make corresponding adjustments in time to ensure the enrichment and cultivation of anaerobic ammonia-oxidizing bacteria and the treatment efficiency of sewage.

[0034] The biological reaction tank 1 is provided with a water inlet and a water outlet, which are respectively located at the two ends of the biological reaction tank 1, the water inlet is located at the bottom of the biological reaction tank 1, and the water outlet is located at the top of the biological reaction tank 1. When the water inlet is set at the bottom of the reaction tank, the sludge will be precipitated accordingly under the action of gravity, and the sludge in the biological reaction tank 1 is more easily enriched; when the water outlet is set at the top of the biological reaction tank 1, the sludge and water will be separated under the action of gravity, so that more water is discharged from the water outlet, reducing the loss of sludge in the biological reaction tank 1.

[0035] The water inlet is connected to a water inlet pipe 4, which is used to transport high-concentration ammonia nitrogen wastewater into the biological reaction pool 1. The high-concentration ammonia nitrogen wastewater treated in this scheme is leachate from a waste incineration plant. The water inlet pipe 4 is provided with a water inlet pump 5 and a water inlet valve 6, and the water inlet valve 6 is used to control the water inflow of the biological reaction pool 1.

[0036] The water outlet is connected to a water outlet pipe 24 , which is used to discharge the filtered liquid. The water outlet pipe 24 is provided with a water outlet valve 25 , a water outlet flow meter 23 and a water pump 29 .

[0037] A partition 9 is provided in the biological reaction tank 1, which divides the biological reaction tank 1 into a plurality of cells. A reaction channel is formed between the partition 9 and the biological reaction tank 1. The reaction channel is wavy or spiral. The partition 9 extends the flow path of the wastewater in the biological reaction tank 1, increases the reaction time between the wastewater and the microorganisms, is beneficial to the reproduction of the microorganisms, and improves the treatment effect of high-concentration ammonia nitrogen wastewater.

[0038] A stuffing box 7 is provided in the middle of each cell. A blank stuffing is built into the stuffing box 7. The stuffing 33 is a polyethylene sponge stuffing string. The addition rate of the stuffing 33 is 40% to 50%. Microorganisms adhere to the stuffing box 7 for reproduction and growth. The stuffing box 7 increases the contact area between the microorganisms and the wastewater, which is beneficial to the reproduction of the microorganisms and improves the treatment effect of high-concentration ammonia nitrogen wastewater. In this embodiment, a stuffing wire 32 is provided in the stuffing box 7, and the stuffing 33 is fixed on the stuffing wire 32.

[0039] It also includes a stirring blade 3 and a stirring motor 2 that drives the stirring blade 3 to rotate. The stirring blade 3 is used to stir and disturb the dead zone of the bioreactor 1. In order to avoid the dead zone formed by the local fluidity of the bioreactor 1 being too small, the stirring blade 3 is set to stir this part of the area, so that the sludge and microorganisms are mixed better, and the treatment effect of high-concentration ammonia nitrogen wastewater is improved. The stirring motor 2 is installed on the bioreactor 1, and the output shaft of the stirring motor 2 passes through the side wall of the bioreactor 1 and is connected to the stirring blade 3. In this embodiment, the stirring motor 2 and the stirring blade 3 are arranged in the area where adjacent cells are connected.

[0040] A tank body insulation sleeve 14 is arranged on the outer wall of the bioreactor 1, and a heater is arranged in the cavity of the tank body insulation sleeve 14. The tank body insulation sleeve 14 reduces the heat loss of the heater and saves energy. At the same time, the tank body insulation sleeve 14 can play a certain protective role for the heater.

[0041] An aeration plate 12 is provided at the bottom of the biological pool, and the aeration plate 12 is connected to the aeration pump through an aeration pipe 19. The aeration pump is located outside the biological reaction pool 1. The aeration pipe 19 is provided with an air volume regulating valve 11 and an aeration flow meter 20. The aeration volume in the biological reaction pool 1 is controlled by the air volume regulating valve 11 and the aeration flow meter 20. The biological reaction pool 1 is aerated by the aeration pump and the aeration plate 12 to adjust the oxygen concentration in the biological reaction pool 1. In this embodiment, an aeration plate 12 is provided at the bottom of each cell, and each aeration plate 12 is connected to the aeration pipe 19. In this embodiment, the aeration pump is specifically a blower 10.

[0042] The filter element 15 is used for enriching the anaerobic ammonium oxidation granular sludge of the biological reaction tank 1. The filter element 15 includes a disc-shaped hollow ceramic membrane 22 and a hollow rotating shaft 13. The hollow rotating shaft 13 is horizontally or tiltedly arranged at the outlet end of the biological reaction tank 1 to ensure that the liquid level is submerged by the disc-shaped hollow ceramic membrane 22. One end of the hollow rotating shaft 13 is closed, and the other end of the hollow rotating shaft 13 is provided with a drain. The disc-shaped hollow ceramic membranes 22 are sleeved on the hollow rotating shaft 13 in parallel with each other. The surface of the hollow rotating shaft 13 is provided with a through hole connected to the disc-shaped hollow ceramic membrane 22, and the drain is connected to the outlet of the biological reaction tank 1. Wastewater in the biological reaction tank 1 enters the filter channel of the disc-shaped hollow ceramic membrane 22 from the surface thereof. Under the action of the disc-shaped hollow ceramic, large particles gradually adhere to the disc-shaped hollow ceramic membrane, and the liquid enters the hollow rotating shaft 13 from the filter channel of the disc-shaped hollow ceramic membrane, and then is discharged from the water outlet of the biological reaction tank 1 through the hollow rotating shaft 13. The disc-shaped hollow ceramic membrane effectively intercepts the anaerobic ammonia oxidation granular sludge, and the loss of anaerobic ammonia oxidation bacteria is avoided to the greatest extent, and a high-concentration microbial flora with stable performance and rich population is formed in the reactor to ensure the denitrification efficiency. At the same time, the hollow rotating shaft 13 is preferably arranged horizontally so that the disc-shaped hollow ceramic membrane 22 can be vertically submerged in the sewage. While the biological reaction tank 1 enriches the anaerobic ammonia oxidation granular sludge, it is prevented that the sludge continues to be enriched near the disc-shaped hollow ceramic membrane 22 after falling off.

[0043] The pore size of the disc-shaped hollow ceramic membrane 22 is 0.05 μm to 2 μm, and the material is selected from one or more of aluminum oxide, zirconium oxide, titanium dioxide and silicon oxide.

[0044] The rotary motor 21 is also included. The rotary motor 21 is connected to the closed end of the hollow rotating shaft. The rotary motor 21 drives the hollow rotating shaft 13 to rotate, so that the disc-shaped hollow ceramic membrane 22 rotates together, so that the surface of the disc-shaped hollow ceramic membrane 22 is not easy to form a filter cake, and the membrane clogging is avoided to the greatest extent. In this embodiment, the rotary motor 21 drives the disc ceramic membrane to rotate at a speed of 400 to 800 rpm / min, preferably 500 r / min, and the pumping pressure is 0.1 MPa to 0.6 MPa, preferably 0.1 MPa. The membrane filtration flux of the disc-shaped hollow ceramic membrane 22 is controlled at 100 to 200 L / m 2 / h.

[0045] It also includes a backwashing assembly for backwashing the disc-shaped hollow ceramic membrane 22, which includes a backwashing pump 27, a backwashing valve 26 and a water storage tank 31. The bottom of the water storage tank 31 is connected to the outlet pipe 24 through a backwashing pipe. The backwashing pump 27 and the backwashing valve 26 are arranged on the backwashing pipe, and the drainage end of the outlet pipe 24 is connected to the top of the water storage tank 31. The disc-shaped hollow ceramic membrane 22 is backwashed regularly through the backwashing assembly at the drainage end of the hollow rotating shaft 13 to prevent the formation of a thick filter cake layer that causes the membrane flux to further decrease; the disc-shaped hollow ceramic membrane and the hollow rotating shaft 13 are backwashed with water filtered by the biological reactor 1 to save water resources. The water storage tank 31 is also connected to a drainage pipe 30, and the water in the water storage tank 31 can be quickly discharged when backwashing is not needed.

[0046] The outlet pipe 24 is also provided with a pressure gauge 28, through which the pumping pressure of the pump 29 can be adjusted to control the backwashing pressure of the filter element 15. In this embodiment, the backwashing pressure is generally set to 0.4 MPa, the backwashing cycle is 30 minutes, and the duration is 10 seconds.

[0047] It also includes a return sludge pipe 16, a sludge return pump 18 and a sludge return valve 17. One end of the return sludge pipe 16 is located in a cell with a water outlet in the biological reaction tank 1, and the other end of the return sludge pipe 16 is located in a cell with a water inlet. The sludge return pump 18 and the sludge return valve 17 are arranged on the return sludge pipe 16, so that the sludge enriched by the filter element 15 is returned to the water inlet end, increasing the microbial concentration at the water inlet end of the biological reaction tank 1 and improving the treatment effect of high-concentration ammonia nitrogen wastewater. In this embodiment, the sludge return pump 18 is a mud pump.

[0048] The present invention also provides a method for enriching and culturing anaerobic ammonia-oxidizing bacteria, comprising the following steps:

[0049] Step 11: First, the activated sludge from the municipal sewage plant or the sludge with anaerobic ammonia oxidation activity is injected into the biological pool, so that the sludge concentration in the biological reaction pool 1 reaches 4500-5000 mg / L.

[0050] Among them, the sludge with anaerobic ammonium oxidation activity comes from the mixture of anaerobic ammonium oxidation seed sludge and anaerobic activated sludge from the sewage treatment plant.

[0051] Step 12: Start the stirring motor 2, which drives the stirring blades to stir the inside of the bioreactor 1, and the rotation speed of the stirring blades 3 is 100-200 rpm; at the same time, turn on the heater to heat the bioreactor 1, and the heating temperature is 30-35°C.

[0052] Step 13: Turn on the water inlet pump 5, which extracts high-concentration ammonia nitrogen wastewater (leachate from a waste incineration plant) and injects it into the bioreactor 1. The wastewater flows along the partition 9 to form a wavy reaction channel. Under the action of the sludge with anaerobic ammonia oxidation activity, the ammonia nitrogen in the high-ammonia nitrogen wastewater is short-range nitrified, and the ammonia oxidizing bacteria in the bioreactor 1 continue to grow. Keep the liquid level in the bioreactor 1 higher than the upper end of the disc-shaped hollow ceramic membrane 22, completely submerge the disc-shaped hollow ceramic membrane 22, start the rotating motor 21 and the pump 29 to discharge water, adjust the outlet water flow rate to be consistent with the inlet water flow rate, and the wastewater in the bioreactor 1 is filtered through the disc-shaped hollow ceramic membrane 22 and the hollow rotating shaft 13. Most of the sludge with anaerobic ammonia oxidation activity is retained in the bioreactor filter, and the filtered liquid is discharged from the outlet to achieve the enrichment of anaerobic ammonia oxidation granular sludge in the bioreactor 1.

[0053] Among them, the influent ammonia nitrogen concentration is 500-800 mg / L, the pH value is 7.8-8.4, and the influent pH is lower than 7.8 and is increased by adding sodium bicarbonate.

[0054] Step 14: Monitor the real-time changes of pH, temperature and dissolved oxygen in the bioreactor 1 through the online monitoring probe 8.

[0055] Step 15: Start the backwash pump 27 within a preset time period to backwash the disc-shaped hollow ceramic membrane 22 and the hollow rotating shaft 13. In this embodiment, the backwash pressure is set to 0.4 MPa, the backwash cycle is 30 minutes, and the duration is 10 seconds.

[0056] Step 21: Continuously monitor the nitrite-nitrogen concentration in the effluent of the biological reactor 1, and adjust the residence time of the wastewater in the biological reactor 1 according to the nitrite-nitrogen concentration in the effluent to accelerate the granulation of the anaerobic ammonia-oxidizing bacteria.

[0057] In this step, the initial wastewater in the biological reactor 1 stays for 28 hours. When the nitrite nitrogen concentration in the effluent is lower than 20 mg / L, the wastewater retention time is gradually shortened and the influent ammonia nitrogen load is continuously increased to quickly increase the ammonia nitrogen volume load of the biological reactor 1, enhance the hydraulic shear force, and accelerate the granulation of the anaerobic ammonia-oxidizing bacteria.

[0058] Step 22: Start the aeration pump to aerate the bioreactor 1 through the aeration plate 12 to control the dissolved oxygen in the bioreactor 1 to be 0.25-0.5 mg / L, preferably 0.4-0.5 mg / L.

[0059] Step 23: Start the sludge return valve 17 and the sludge return pump 18, and control the sludge return ratio according to the granular sludge formation situation.

[0060] The sludge internal reflow ratio is controlled according to the granular sludge formation situation, and the internal reflow ratio is less than 300%.

[0061] Step 24: When the ammonia nitrogen and total nitrogen removal load of the entire device reaches 0.1kg / (m 3 ·d), and the effluent nitrite nitrogen concentration is lower than 20 mg / L, the sludge acclimation culture is completed.

[0062] In this embodiment, at the end of the sludge acclimatization culture, the concentrations of ammonia nitrogen, nitrite nitrogen and nitrate nitrogen in the drainage compartment at the end of the biological reaction tank 1 are preferably controlled to be below 50 mg / L for ammonia nitrogen and 20 mg / L for nitrite.

[0063] In this embodiment, after 40 to 50 days of cumulative cultivation, the residence time of the leachate from the garbage incineration plant is shortened to 16 hours, at which time the ammonia nitrogen removal rate reaches 90%, and the system operation is considered to be relatively stable, and the enrichment culture of anaerobic ammonia oxidizing bacteria is completed. In the enrichment culture stage, the inhibition of low dissolved oxygen, free ammonia, and free nitrite is used to achieve short-range nitrification of ammonia nitrogen in high ammonia nitrogen sewage; the gradient change of ammonia nitrogen concentration in each compartment in the biological reaction tank 1 and the continuous reflux of sludge are strengthened, so that the short-range nitrification reaction can be stably maintained, that is, the growth rate of ammonia oxidizing bacteria is greater than the growth rate of nitrite oxidizing bacteria, so that nitrite oxidizing bacteria are continuously suppressed and washed out, the short-range nitrification efficiency is strengthened, and the nitrification volume load is improved. A built-in filter element 15 is provided in the biological reaction tank 1, and through the rising flow rate, the liquid-solid efficient separation of wastewater and granular sludge in each compartment can be achieved, large granular sludge is intercepted, and floc sludge is washed to achieve the effect of sludge screening.

[0064] Step 3: Anaerobic ammonium oxidizing microorganisms continue to reproduce the high ammonia nitrogen wastewater treatment: The anaerobic ammonium oxidizing microorganisms attached to the filler of the biological reactor 1 continue to reproduce the high ammonia nitrogen wastewater treatment.

[0065] The packing wires 32 and packing 33 in the biological reactor 1 can attach a large number of anaerobic ammonia-oxidizing microorganisms during operation, which can provide a basis for the stable operation of the device. The forced interception effect of the filter element 15 is used to ensure that the sludge does not flow out, eliminating the three-phase separation structure of the traditional upflow anaerobic sludge blanket (UASB) reactor and overcoming the sludge leakage problem of the continuous flow reactor.

[0066] This scheme combines the rapid cultivation of anaerobic ammonia-oxidizing bacteria with cross-flow filtration of ceramic membranes to effectively treat high-concentration, high-suspended-matter organic wastewater. The high-speed rotation of the ceramic membrane can effectively control the formation of a filter cake layer on the membrane surface, and can maintain a stable flux even after long-term operation. In the reactor, the enrichment of anaerobic ammonia-oxidizing bacteria is enhanced by the interception effect of the membrane, and the self-coagulation effect of anaerobic ammonia-oxidizing bacteria is used to achieve the mixed form of granular sludge and mud film culture, breaking through the technical bottleneck of easy attenuation and loss of bacteria, and providing guarantee for engineering application. Granular sludge with denitrification function can be quickly cultivated through the coexistence of aerobic ammonia-oxidizing bacteria, anaerobic ammonia-oxidizing bacteria and denitrifying bacteria, effectively avoiding membrane pollution while achieving efficient biological denitrification. It can achieve efficient separation of solid, liquid and gas, effectively intercept anaerobic ammonia-oxidizing granular sludge, avoid the loss of anaerobic ammonia-oxidizing bacteria in the system, and at the same time tolerate a higher influent load to ensure that the effluent suspended matter is less than 100 mg / L. The device is suitable for cultivating slow-growing anaerobic ammonia-oxidizing bacteria, maximizing the residence time of the sludge, thereby increasing the deammoniation load of the device to 0.5g NH 4+ / L / d, it has the advantages of high effective volume utilization and stable effluent quality. It is suitable for the denitrification treatment of industrial wastewater with high ammonia nitrogen, low carbon-nitrogen ratio and high suspended solids.

[0067] The specific shape of the reaction flow channel can be changed according to the actual situation and is not limited to the aforementioned shape. Other similar reagents and the selection of similar reaction parameters can be determined by those skilled in the art based on common knowledge. The above is only an embodiment of the present invention. The common sense such as the known specific structure, characteristics and reactant ratio in the scheme is not described too much here. It should be pointed out that for those skilled in the art, without departing from the concept of the present invention, several deformations and improvements can be made, such as simply adjusting the parameters within or near the specified parameter range, which should also be regarded as the scope of protection of the present invention, which will not affect the effect of the implementation of the present invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods in the specification and other records can be used to explain the content of the claims.

Claims

1. A device for enriching and culturing anaerobic ammonia-oxidizing bacteria, characterized in that: include: A biological reaction tank, a heater, an online monitoring probe, and a filter element arranged in the biological reaction tank, wherein the online monitoring probe is used to monitor the pH value, temperature value and dissolved oxygen content in the biological reaction tank; the heater is used to heat the biological reaction tank, the biological reaction tank is provided with a water inlet and a water outlet, the filter element is used for enriching anaerobic ammonia oxidation granular sludge in the biological reaction tank, and the drainage port of the filter element is connected to the water outlet.

2. The device for enriching and culturing anaerobic ammonia-oxidizing bacteria according to claim 1, characterized in that: A partition is arranged in the biological reaction pool, and the partition divides the biological reaction pool into a plurality of cells, and a reaction channel is formed between the partition and the biological reaction pool.

3. The device for enriching and culturing anaerobic ammonia-oxidizing bacteria according to claim 2, characterized in that: It also includes a stirring blade and a stirring motor for driving the stirring blade to rotate. The stirring motor and the stirring blade are used for stirring and disturbing the dead zone of the biological reaction tank.

4. The device for enriching and culturing anaerobic ammonia-oxidizing bacteria according to claim 1, characterized in that: The water inlet is located at the bottom of the biological reaction tank, and the water outlet is located at the top of the biological reaction tank.

5. The device for enriching and culturing anaerobic ammonia-oxidizing bacteria according to claim 1, characterized in that: A stuffing box is arranged in the biological reaction pool, and stuffing wires and stuffing are arranged in the stuffing box, and the stuffing is fixed on the stuffing wires.

6. The device for enriching and culturing anaerobic ammonia-oxidizing bacteria according to claim 1, characterized in that: It also includes an aeration pump and an aeration plate. The aeration pump and the aeration plate are connected, and the aeration plate is located at the bottom of the biological reaction tank.

7. The device for enriching anaerobic ammonia-oxidizing bacteria according to claim 1, characterized in that: The filter element includes a disc-shaped hollow ceramic membrane and a hollow rotating shaft. The hollow rotating shaft is rotatably arranged on the bioreactor. The disc-shaped hollow ceramic membranes are sleeved on the hollow rotating shaft in parallel with each other. A through hole connected to the disc-shaped hollow ceramic membrane is provided on the surface of the hollow rotating shaft. One end of the hollow rotating shaft is closed, and the other end of the hollow rotating shaft is provided with a drain outlet, which is connected to the water outlet of the bioreactor.

8. The device for enriching anaerobic ammonium-oxidizing bacteria according to claim 7, characterized in that: It also includes a backwashing component for backwashing the disc-shaped hollow ceramic membrane, and the backwashing component is connected to the drain port of the hollow rotating shaft.

9. The device for enriching anaerobic ammonia-oxidizing bacteria according to claim 7, characterized in that: It also includes a return sludge pipe and a sludge return pump, which is arranged on the return sludge pipe and is used to pump the sludge enriched by the disc-shaped hollow ceramic membrane at the outlet end to the water inlet end.

10. A method for enriching and culturing anaerobic ammonia-oxidizing bacteria, characterized in that: The following steps are involved: Step 1: Inoculate sludge to start the bioreactor: Inoculate the bioreactor with activated sludge from a municipal sewage plant or sludge with anaerobic ammonium oxidation activity to make the sludge concentration in the bioreactor reach 4500-5000 mg / L, use a continuous flow mode to transport the leachate from the garbage incineration plant into the bioreactor, the effluent flow rate of the bioreactor is consistent with the inlet flow rate, start the stirring motor and stirring blades to stir the inside of the bioreactor, heat the bioreactor, the heating temperature is 30-35°C, and the stirring blade speed is 100-200rpm / min; the filter element enriches the anaerobic ammonium oxidation granular sludge in the bioreactor; Step 2: Enrichment and cultivation of anaerobic ammonium oxidizing microorganisms: Continuously monitor the nitrite nitrogen concentration in the effluent of the bioreactor, and adjust the residence time of the wastewater in the bioreactor according to the nitrite nitrogen concentration in the effluent to accelerate the granulation of anaerobic ammonium oxidizing bacteria; and control the dissolved oxygen in the bioreactor to 0.4-0.5 mg / L, and the ammonia nitrogen and total nitrogen removal load to 0.1 kg / (m 3 ·d), and the effluent nitrite nitrogen concentration is lower than 20 mg / L, the sludge acclimation culture is completed; Step 3: Anaerobic ammonium oxidizing microorganisms continue to reproduce stably in the treatment of high ammonia nitrogen wastewater: The anaerobic ammonium oxidizing microorganisms attached to the filler of the biological reactor continue to reproduce in the treatment of high ammonia nitrogen wastewater.

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