Chiral molecule chiral amplification detection device and method based on optical microflow laser

Abstract

The invention belongs to the technical field of biological detection, and particularly relates to a chiral molecule chiral amplification detection device and method based on optical microflow laser. The device comprises a pump laser, pump laser output by the pump laser is changed into linearly polarized light after passing through a polarization splitting prism, the linearly polarized light is changed into circularly polarized light after passing through a first quarter-wave plate, and the circularly polarized light is focused to a light micro-flow laser after passing through a dichroic sheet and a first lens; laser emitted by molecules to be detected in the optical micro-flow laser after being excited is divided into two beams through the first lens, the dichroic sheet and the first optical splitter, one beam is received by the CCD, and the other beam is received by the spectrograph. According to the invention, the laser signal is amplified in the optical microcavity, so that the chiral characteristic of the chiral molecule is amplified, and the precision of chiral detection can be improved.

Description

Technical Field

[0001] The invention belongs to the technical field of biological detection, and in particular relates to a device and method for detecting the chirality of chiral molecules based on optical microfluidic laser. Background Art

[0002] Optical microfluidic laser is an important application of optical microfluidics in lasers. Optical microfluidics is a highly integrated and small-volume analysis system designed by combining optical components and microfluidic chips. Optical microfluidic laser technology has the advantages of high sensitivity, low cost, high throughput, and easy integration. The technology of optical microfluidic laser combines optical microfluidics and microfluidics: by integrating the optical resonant cavity into the microsystem, using fluorescent dyes and target detection substances as gain media, and through the amplification of the optical resonator, the fluorescent signal can be converted into a laser signal, and the intensity of the emitted laser is used as a sensing signal to achieve the detection of the target detection object. Laser-based detection can amplify the tiny but inherent differences between two molecules, generating differential signals that are several orders of magnitude larger than fluorescence-based methods, and the same is true for detecting chiral materials.

[0003] Chiral substances are widely present in nature. Some common chiral substances in nature include proteins, amino acids, DNA, etc. GFP is a common chiral fluorescent dye molecule, and many drug molecules also have chirality. Due to the existence of chirality, chiral compounds will show two different configurations, called enantiomers, and one configuration is often beneficial and the other is harmful. When measuring chiral molecules, CD spectra and CPL spectra are usually used. CD spectra are used to characterize the difference in the absorption ability of molecules to circularly polarized light, that is, when a molecule has chirality, the molecule has different absorption abilities to left-handed circularly polarized light (CPL) and right-handed circularly polarized light (LCP). CPL spectra refer to the intensity difference ratio of left-handed circularly polarized light (CPL) and right-handed circularly polarized light (LCP) emitted by chiral molecules, that is, the asymmetry factor.

[0004] Therefore, in order to accurately and efficiently detect such chiral molecules, it is of great significance to amplify the chirality of these molecules. In order to amplify the asymmetry factor, it is usually based on spontaneous radiation or passive resonant cavity, such as using self-assembly and chiral transfer properties to show strong inherent molecular chirality, manipulating local fields to enhance chiral signals such as plasmons and metasurfaces, and using standing waves of two opposite chiral lights to improve optical enantioselectivity. However, these methods are all based on the fluorescence signal of chiral molecules, and the maximum asymmetry factor that can be obtained is 10. -1 The magnitude of the detection is limited. Summary of the invention

[0005] In order to solve the technical problem of limited accuracy in measuring the chirality of chiral molecules in the prior art, the present invention proposes a chiral molecule chirality amplification detection device and method based on optical microfluidic laser, which can increase the asymmetry factor of chiral molecules by several orders of magnitude and greatly improve the detection accuracy.

[0006] In order to solve the above technical problems, the technical solution adopted by the present invention is: a chiral molecule chirality amplification detection device based on optical microfluidic laser, comprising: a pump laser, a polarization beam splitter prism, a first quarter wave plate, a dichroic plate, a first lens, a sample stage, an optical microfluidic laser, a first beam splitter, a spectrometer and a CCD;

[0007] The optical microfluidic laser is arranged on a sample stage, and comprises a first cavity mirror, a gasket, and a second cavity mirror. The first cavity mirror is connected to the second cavity mirror through two gaskets to form a channel. A plurality of concave structures are arranged on the first cavity mirror located above the channel to form a concave array. The surfaces opposite to the first cavity mirror and the second cavity mirror are both provided with a coating layer to form a cavity array including a plurality of plano-concave FP cavities. The channel is used to arrange a gain medium including a molecule to be detected.

[0008] The pump laser output by the pump laser is converted into linear polarized light after passing through a polarization beam splitter prism, the linear polarized light is converted into circular polarized light after passing through a first quarter wave plate, the circular polarized light is focused to the optical microfluidic laser after passing through a dichroic plate and a first lens, the laser light emitted by the molecules to be measured in the optical microfluidic laser is stimulated and emitted through a first lens, a dichroic plate and a first beam splitter and is divided into two beams, one of which is received by a CCD and the other is received by a spectrometer.

[0009] In the optical microfluidic laser, a conduit communicating with the channel is arranged on one of the cavity mirrors, and the conduit is used to introduce a liquid gain medium.

[0010] The chiral molecular chirality amplification detection device based on optical microfluidic laser also includes a second spectrometer and a power meter. The second spectrometer is arranged between the polarization spectrometer and the first quarter-wave plate, and is used to separate a part of the linearly polarized light to the power meter to monitor the intensity of the pump laser.

[0011] The chiral molecular chirality amplification and detection device based on optical microfluidic laser also includes an attenuation plate, which is arranged at the output end of the polarization beam splitter prism and is used to attenuate the intensity of the linearly polarized light output by the polarization beam splitter prism.

[0012] The chiral molecule chirality amplification detection device based on optical microfluidic laser also includes a first polarizer, which is arranged between the first quarter-wave plate and the dichroic plate and is used to check whether the laser output by the first quarter-wave plate is circularly polarized light.

[0013] The chiral molecule chirality amplification detection device based on optical microfluidic laser also includes a second quarter-wave plate, a second polarizer and a second lens. The laser light emitted by the molecules to be detected in the optical microfluidic laser is stimulated and then divided into two beams after passing through the first lens, the dichroic plate and the first beam splitter, one of which is received by the CCD and the other is received by the spectrometer through the second quarter-wave plate, the second polarizer and the second lens in sequence.

[0014] In addition, the present invention also provides a method for detecting the chirality of chiral molecules based on optical microfluidic laser, which is implemented based on the aforementioned chiral molecule chirality amplification detection device based on optical microfluidic laser, and includes the following steps:

[0015] Step 1: After the gain medium sample is prepared, it is injected into the FF-P cavity in the optical microfluidic laser;

[0016] Step 2: Change the angle of the first quarter wave plate so that the included angle between its fast axis and the linearly polarized light output by the polarization beam splitter prism is 45°, and collect the generated laser signal through a spectrometer;

[0017] Step 3: Change the angle of the quarter wave plate again so that the angle between its fast axis and the linearly polarized light output by the polarization beam splitter is -45°, and collect the generated laser signal through the spectrometer;

[0018] Step 4: Calculate the characteristic parameters of the chiral molecule based on the spectral data measured in steps 2 and 3 respectively.

[0019] The calculation formula of the characteristic parameter is:

[0020] glum = 2 × (IL-IR) / (IL+IR);

[0021] Where glum represents the asymmetry factor, IL and IR represent the intensity of the emitted light under the excitation of left-handed circularly polarized light and right-handed circularly polarized light, respectively.

[0022] The step 1 also includes the following steps: adjusting the intensity of the pump laser incident on the optical microfluidic laser through an attenuation plate.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] The present invention proposes a chiral molecule chirality amplification detection device and method based on optical microfluidic laser, which utilizes laser signals to perform signal amplification in an optical microcavity, thereby amplifying the chiral characteristics of chiral molecules, thereby improving the accuracy of chiral detection. Experiments have confirmed that when a solution of double-stranded DNA combined with a saturated fluorescent dye is used as a gain medium, the asymmetry factor obtained by the present invention is increased by several orders of magnitude compared to fluorescence detection. Therefore, the present invention greatly improves the chiral detection accuracy of chiral molecules. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 A schematic structural diagram of a chiral molecular chirality amplification device based on optical microfluidic laser provided in an embodiment of the present invention;

[0026] Figure 2 Schematic diagram of the structure of the optical microfluidic laser used in the embodiment of the present invention;

[0027] Figure 3 It is a schematic diagram of the concave structure of the reflector in the FP cavity of the optical microfluidic laser in the example of the present invention;

[0028] Figure 4 is the fluorescence spectrum of SYTO13 dye molecule;

[0029] In the figure: 1 is the first cavity mirror, 2 is the coating layer, 3 is the second cavity mirror, 4 is the gasket, 5 is the catheter, 6 is the channel, 7 is the micro-concave structure, 8 is the pump laser, 9 is the polarization beam splitter prism, 10 is the attenuation plate, 11 is the power meter, 12 is the second beam splitter, 13 is the first quarter wave plate, 14 is the first polarizer, 15 is the sample stage, 16 is the optical microfluidic laser, 17 is the first lens, 18 is the dichroic plate, 19 is the first beam splitter, 20 is the CCD, 21 is the second quarter wave plate, 22 is the second polarizer, 23 is the second lens, and 24 is the spectrometer. DETAILED DESCRIPTION

[0030] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all the embodiments; based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0031] Embodiment 1

[0032] like Figure 1 As shown, embodiment 1 of the present invention provides a chiral molecule chirality amplification and detection device based on optical microfluidic laser, including: a pump laser 8, a polarization beam splitter prism 9, a first quarter-wave plate 13, a dichroic plate 18, a first lens 17, a sample stage 15, an optical microfluidic laser 16, a first beam splitter 19, a spectrometer 24 and a CCD 20.

[0033] The optical microfluidic laser 16 is arranged on the sample stage 15. The pump laser outputted by the pump laser 8 is converted into linear polarized light after passing through the polarization beam splitter prism 9, and the linear polarized light is converted into circular polarized light after passing through the first quarter wave plate 13, and the circular polarized light is focused to the optical microfluidic laser 16 after passing through the dichroic plate 18 and the first lens 17, and the laser light emitted by the molecules to be measured in the optical microfluidic laser 16 is divided into two beams after passing through the first lens 17, the dichroic plate 18 and the first beam splitter 19, one of which is received by the CCD 20, and the other is received by the spectrometer 24.

[0034] like Figures 2-3 As shown, in this embodiment, the optical microfluidic laser 16 includes a first cavity mirror 1, a gasket 4 and a second cavity mirror 3. The first cavity mirror 1 is connected to the second cavity mirror 3 through two gaskets 4 to form a channel 6. A plurality of concave structures 7 are arranged on the first cavity mirror 1 above the channel 6 to form a concave array. The surfaces opposite to the first cavity mirror 1 and the second cavity mirror 3 are both provided with a coating layer 2 to form a cavity array including a plurality of plano-concave FP cavities. The gain medium including the molecule to be measured is arranged in the channel 6. The coating layer 2 arranged on the first cavity mirror 1 and the second cavity mirror 3 has a reflectivity greater than 99.9% at the laser wavelength emitted by the excitation.

[0035] Furthermore, if Figure 2 As shown, in this embodiment, a conduit 5 communicating with the channel 6 is disposed on one of the cavity mirrors of the optical microfluidic laser 16 , and the conduit 5 is used to pass a liquid gain medium.

[0036] Furthermore, if Figure 1 As shown, a chiral molecule chirality amplification detection device based on optical microfluidic laser in this embodiment also includes a second beam splitter 12 and a power meter 11. The second beam splitter 12 is arranged between the polarization beam splitter prism 9 and the first quarter-wave plate 13, and is used to separate a part of the linearly polarized light to the power meter 11 to monitor the intensity of the pump laser.

[0037] Furthermore, if Figure 1 As shown, the chiral molecular chirality amplification detection device based on optical microfluidic laser of this embodiment further includes an attenuation plate 10, which is arranged at the output end of the polarization beam splitter prism 9 and is used to attenuate the linear polarized light intensity output by the polarization beam splitter prism 9. Specifically, the attenuation plate 10 is a continuously variable neutral density filter.

[0038] Furthermore, if Figure 1 As shown, a chiral molecule chirality amplification detection device based on optical microfluidic laser in this embodiment also includes a first polarizer 14, which is arranged between the first quarter-wave plate 13 and the dichroic plate 18, and is used to check whether the laser output by the first quarter-wave plate 13 is circularly polarized light.

[0039] Furthermore, if Figure 1 As shown, a chiral molecule chirality amplification detection device based on optical microfluidic laser of this embodiment also includes a second quarter wave plate 21, a second polarizer 22 and a second lens 23. The laser light emitted by the molecules to be detected in the optical microfluidic laser 16 is stimulated to pass through the first lens 17, the dichroic plate 18 and the first beam splitter 19 and is divided into two beams, one of which is received by the CCD 20, and the other is received by the spectrometer 24 through the second quarter wave plate 21, the second polarizer 22 and the second lens 23 in sequence.

[0040] Specifically, in this embodiment, the pump laser 8 is an OPO tunable laser, and the emission wavelength is set to 488nm. The first beam splitter 19 and the second beam splitter 12 can be beam splitting prisms. In this embodiment, the coating layer provided on the first cavity mirror 1 and the second cavity mirror 3 is a Bragg reflection film, which is composed of 15 layers of Ti2O5 and SiO2 dielectric layers alternating, and its reflectivity R>99.9%, and its reflection band is between 500-560nm. The width of the microfluidic channel 6 is 10mm.

[0041] The steps for preparing the optical microfluidic laser 16 including the FP cavity array in this embodiment are as follows:

[0042] (1) Figure 3 As shown, a micro-concave structure 7 is processed on a flat quartz glass using a CO2 laser, and the FP cavity is a 3*3 micro-concave array processed on a quartz glass plane of 2cm*2cm*1mm.

[0043] (2) Coating: The quartz glass with the micro-concave structure 7 and the quartz glass without concave structure are processed by ion beam sputtering to form a coating layer 2, so that the reflectivity R in the reflection band between 500-560nm is as high as 99.9%.

[0044] (3) Packaging of FP cavity: The specific cavity length is 30um. Two 30um gaskets 4 are selected and placed on both sides of the middle of the two reflective cavity mirrors. The two coated quartz glasses are packaged to form a laser resonant cavity. A channel 6 for storing liquid medium is left in the middle. A round hole is left on the first cavity mirror 1. The hose 5 is inserted into the small hole and glued with glue to inject the gain medium liquid molecules to be tested. No sample is wasted and the sample can be fully injected into the FP cavity.

[0045] The working principle of this embodiment is as follows:

[0046] The pump laser 8 emits a 488nm pump light as horizontal linear polarized light. First, the pump light passes through a polarization beam splitter prism 9 to convert the pump light into a completely horizontal linear polarized light. Then, the light intensity is adjusted by the attenuation plate 10. The pump light of different intensities can be obtained by rotating the attenuation plate 10. Then, a beam of detection light is reflected and separated by a 50 / 50 second beam splitter 12, and the light intensity of the pump light is detected by an optical power meter 11. Another beam of transmitted light passes through a first quarter wave plate 13. The first quarter wave plate 13 is used to obtain circularly polarized light. After the linear polarized light passes through the λ / 4 wave plate, circularly polarized light will be generated. According to the different polarization directions of the incident pump light and the angles of the fast axis of the wave plate, left-handed circularly polarized light can be obtained respectively. When a beam of horizontal linear polarized light is viewed facing the light beam and forms an angle of 45° with the fast axis direction of the wave plate, right-handed circularly polarized light will be emitted. When the beam of horizontal linear polarized light is viewed facing the light beam and forms an angle of -45° with the fast axis direction of the wave plate, left-handed circularly polarized pump light will be generated. The circularly polarized pump light generated by the first quarter-wave plate 13 will be reflected by the dichroic plate 18 and enter the optical microfluidic laser 16 equipped with the sample on the sample stage 15. The sample is stimulated to emit laser light under the pumping of the circularly polarized light. The emitted laser light passes through the dichroic plate 18. After passing through the dichroic plate 18, the laser light will then pass through a second beam splitter 19 with a splitting ratio of 3 / 7. 30% of the light will enter the CCD 20, and 70% of the light will pass through the second quarter-wave plate 21. At this time, the emitted circularly polarized laser light will become a linearly polarized laser light, and then enter the linear polarizer 22 for polarization analysis. At this time, the emitted linearly polarized laser light will have obvious differences in intensity under the polarization direction of 0° and the polarization direction of 90°. After being converted into linearly polarized light, it is focused onto the optical fiber through the second lens 23 and enters the spectrometer 24 for spectral analysis.

[0047] Embodiment 2

[0048] Embodiment 2 of the present invention provides a method for detecting the chirality of chiral molecules based on optical microfluidic laser, which is implemented based on the chirality detection device for chiral molecules based on optical microfluidic laser described in Embodiment 1, and includes the following steps:

[0049] Step 1: After the gain medium sample is prepared, it is injected into the FF-P cavity in the optical microfluidic laser (16).

[0050] In this embodiment, the biological chiral molecule selected is a double-stranded DNA molecule. The DNA molecule is a chiral molecule commonly seen in daily life. DNA usually exhibits right-handed helicity. The DNA molecule is a molecule with a spirally distributed double helix structure. Specifically, the DNA molecules used in this embodiment are 50 bases in length and 80 bases in length, respectively.

[0051] Further, the dye molecule specifically used in this example is SYTO13 dye molecule, which is an embedded saturated dye molecule, and the dye molecule can be embedded in the double helix structure of the DNA molecule. The excitation wavelength of the SYTO13 dye molecule is 488nm, and the emission wavelength is 532nm, that is, two kinds of quarter wave plates of wavelength are used in the experimental device, one is 488nm and the other is 532nm. The double-stranded DNA molecule chiral fluorescent probe dye molecule is realized by self-assembly method. The saturated dye used in the present invention is SYTO 13 saturated nucleic acid stain (Invitrogen, initially dissolved in DMSO with a concentration of 5mM). Figure 4 Shown is the fluorescence spectrum of SYTO13.

[0052] The steps for preparing the solution are as follows:

[0053] 1. Centrifuge the 50- and 80-base DNA powders in a centrifuge at 4000 r / min for 1 min 30 s to allow the powder to fill the tube and avoid remaining on the tube wall.

[0054] 2. Dissolve the two complementary single-stranded DNA powders after centrifugation in a buffer solution (triacetate-EDTA buffer: PH=8.3), that is, add the buffer solution to the DNA sample to fully dissolve it into a 100UM single-stranded DNA solution, then take equal volumes of the two complementary single-stranded DNA solutions and mix them thoroughly, and place them on an oscillator to mix them thoroughly and evenly.

[0055] In order to ensure that the two complementary single-stranded DNA molecules can be fully paired, the two successfully prepared single-stranded DNA molecular solution samples are then heated to 95°C and then cooled to room temperature. Finally, saturated nucleic acid dye SYTO13 is added for thorough mixing. SYTO13 is calculated at a concentration of 500uM and added to the double-stranded DNA molecular solution for thorough shaking.

[0056] After the samples are prepared, double-stranded DNA molecule samples with a length of 50 bases and a length of 80 bases are tested respectively. During the test, the mixed solution of the sample DNA is injected into the FP cavity through a hose using an injection needle until the entire cavity is filled.

[0057] The 488 nm pump light output by the pump laser 8 passes through a series of optical elements and is finally converged by the first lens 17 to form a light spot of about 80 um on the sample to pump the sample.

[0058] Step 2: Change the angle of the first quarter wave plate 13 so that the included angle between its fast axis and the linearly polarized light output by the polarization beam splitter prism 9 is 45°, and collect the generated laser signal through the spectrometer 24 .

[0059] Step 3: Change the angle of the quarter wave plate 13 again so that the included angle between its fast axis and the linearly polarized light output by the polarization beam splitter prism 9 is −45°, and collect the generated laser signal through the spectrometer 24 .

[0060] Step 4: Calculate the characteristic parameters of the chiral molecule based on the spectral data measured in steps 2 and 3 respectively.

[0061] In step 4, the calculation formula of the characteristic parameter is:

[0062] glum = 2 × (IL-IR) / (IL+IR);

[0063] Among them, glum represents the asymmetry factor, IL and IR represent the outgoing laser intensity under the excitation of left-handed circularly polarized light and right-handed circularly polarized light, respectively. The numerical value of the asymmetry factor obtained by calculation is several orders of magnitude larger than the asymmetry factor based on the previous fluorescence-based detection.

[0064] Specifically, the chiral amplification detection method of chiral molecules based on optical microfluidic laser of this embodiment further includes the following steps: adjusting the intensity of the pump laser incident on the optical microfluidic laser 16 by the attenuation plate 10 .

[0065] In this embodiment, a first polarizer 14 is arranged behind the first quarter wave plate 13 to check whether the emitted pump light is circularly polarized light. If it is circularly polarized light, the emitted light intensity will not change when the first polarizer 14 rotates 360°.

[0066] In this embodiment, left-handed and right-handed circularly polarized lights are obtained respectively by changing the polarization angle between the wave plate fast axis of the first quarter-wave plate 13 and the linearly polarized pump light output by the polarization beam splitter prism 9. When the angle is 45°, right-handed circularly polarized pump light is generated. The intensity of the pump light can be adjusted by adjusting the attenuation plate. After the pump light enters the FP cavity of the optical microfluidic laser 16, it will pump and generate laser light. At this time, the generated laser light is transmitted through the dichroic plate and then incident on the second quarter-wave plate 21 with a wavelength of 532nm, so that the laser light is converted into linearly polarized laser light, and then polarization is analyzed by the second polarizer 22. At this time, the light intensity in the same polarization direction as the second polarizer 22 is the strongest, and the light intensity in the vertical direction is the weakest. Finally, it is collected by the multimode optical fiber and transmitted to the spectrometer for data analysis. On the contrary, if the angle is -45°, left-handed circularly polarized light will be generated. Similarly, a single pulse is used to excite the DNA sample to obtain an output laser signal, which is finally transmitted to the spectrometer through the optical fiber for analysis.

[0067] The present invention proposes a chiral molecule chirality amplification detection device and method based on optical microfluidic laser, which utilizes laser signals to perform signal amplification in an optical microcavity, thereby amplifying the chiral characteristics of chiral molecules, thereby improving the accuracy of chiral detection. Experiments have confirmed that when a solution of double-stranded DNA combined with a saturated fluorescent dye is used as a gain medium, the asymmetry factor obtained by the present invention is increased by several orders of magnitude compared to fluorescence detection. Therefore, the present invention greatly improves the chiral detection accuracy of chiral molecules.

[0068] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A chiral molecule chirality amplification detection device based on optical microfluidic laser, characterized in that: include: A pump laser (8), a polarization beam splitter (9), a first quarter wave plate (13), a dichroic plate (18), a first lens (17), a sample stage (15), an optical microfluidic laser (16), a first beam splitter (19), a spectrometer (24) and a CCD (20); The optical microfluidic laser (16) is arranged on a sample stage (15), and comprises a first cavity mirror (1), a gasket (4) and a second cavity mirror (3); the first cavity mirror (1) is connected to the second cavity mirror (3) via two gaskets (4) to form a channel (6); a plurality of concave structures (7) are arranged on the first cavity mirror (1) above the channel (6) to form a concave array; the surfaces opposite to each other of the first cavity mirror (1) and the second cavity mirror 3 are both provided with a coating layer to form a cavity array comprising a plurality of plano-concave FP cavities; a gain medium comprising a molecule to be detected is arranged in the channel (6); The pump laser output by the pump laser (8) is converted into linear polarized light after passing through a polarization beam splitter prism (9), the linear polarized light is converted into circular polarized light after passing through a first quarter wave plate (13), the circular polarized light is focused onto an optical microfluidic laser (16) after passing through a dichroic plate (18) and a first lens (17), and the laser light emitted by the molecules to be detected in the optical microfluidic laser (16) is divided into two beams after passing through a first lens (17), a dichroic plate (18) and a first beam splitter (19), one of which is received by a CCD (20) and the other is received by a spectrometer (24).

2. The chiral molecular chirality amplification detection device based on optical microfluidic laser according to claim 1, characterized in that: In the optical microfluidic laser (16), a conduit (5) communicating with the channel (6) is arranged on one of the cavity mirrors, and the conduit (5) is used to pass a liquid gain medium.

3. The device for detecting the chiral properties of chiral molecules based on optical microfluidic laser according to claim 1, characterized in that: It also includes a second beam splitter (12) and a power meter (11). The second beam splitter (12) is arranged between the polarization beam splitter prism (9) and the first quarter-wave plate (13) and is used to split a part of the linearly polarized light to the power meter (11) to monitor the intensity of the pump laser.

4. The chiral molecule chirality amplification detection device based on optical microfluidic laser according to claim 1, characterized in that: It also comprises an attenuation plate (10), which is arranged at the output end of the polarization beam splitter prism (9) and is used to attenuate the intensity of the linearly polarized light output by the polarization beam splitter prism (9).

5. The chiral molecule chirality amplification detection device based on optical microfluidic laser according to claim 1, characterized in that: It also comprises a first polarizing plate (14), which is arranged between the first quarter-wave plate (13) and the dichroic plate (18) and is used to check whether the laser light output by the first quarter-wave plate (13) is circularly polarized light.

6. The chiral molecule chirality amplification detection device based on optical microfluidic laser according to claim 1, characterized in that: The invention also comprises a second quarter wave plate (21), a second polarizing plate (22) and a second lens (23). The laser light emitted by the molecules to be detected in the optical microfluidic laser (16) is stimulated to pass through a first lens (17), a dichroic plate (18) and a first beam splitter (19), and then is divided into two beams, one of which is received by a CCD (20), and the other is received by a spectrometer (24) via the second quarter wave plate (21), the second polarizing plate (22) and the second lens (23) in sequence.

7. A method for detecting the chirality of chiral molecules based on optical microfluidic laser, characterized in that: The invention is realized by a chiral molecule chirality amplification detection device based on optical microfluidic laser according to any one of claims 1 to 6, comprising the following steps: Step 1: After the gain medium sample is prepared, it is injected into the FF-P cavity in the optical microfluidic laser (16); Step 2: changing the angle of the first quarter wave plate (13) so that the included angle between its fast axis and the linearly polarized light output by the polarization beam splitter (9) is 45°, and collecting the generated laser signal through a spectrometer (24); Step 3: Changing the angle of the quarter wave plate (13) again so that the angle between its fast axis and the linearly polarized light output by the polarization beam splitter (9) is -45°, and collecting the generated laser signal through the spectrometer (24); Step 4: Calculate the characteristic parameters of the chiral molecule based on the spectral data measured in steps 2 and 3 respectively.

8. The method for detecting the chiral properties of chiral molecules based on optical microfluidic laser according to claim 7, characterized in that: The calculation formula of the characteristic parameter is: glum = 2 × (IL-IR) / (IL+IR); Where glum represents the asymmetry factor, IL and IR represent the intensity of the emitted light under the excitation of left-handed circularly polarized light and right-handed circularly polarized light, respectively.

9. The method for detecting the chiral properties of chiral molecules based on optical microfluidic laser according to claim 7, characterized in that: The step 1 also includes the following steps: adjusting the intensity of the pump laser incident on the optical microfluidic laser (16) through the attenuation plate (10).

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