Bacteriostatic anti-inflammatory high-performance hydrogel and preparation method thereof

Abstract

The invention discloses antibacterial and anti-inflammatory high-performance hydrogel and a preparation method thereof in the technical field of hydrogel. The antibacterial and anti-inflammatory high-performance hydrogel comprises the following components in parts by weight: 50-60 parts of high-performance gel and 6-8 parts of antibacterial slow-release essential oil, the high-performance gel is obtained by crosslinking methacrylic acid modified gelatin and hydroxyethyl methylacrylate loaded with acetylcysteine; the antibacterial slow-release essential oil is prepared from modified kaolinite and a plant extract. According to the invention, acetylcysteine is combined with hydroxyethyl methylacrylate, so that the oxidation resistance of the drug-loaded gel is improved, further the inflammatory reaction is relieved, and meanwhile, acetylcysteine and antibacterial slow-release essential oil are used for replacing antibiotics, so that the antibacterial ability of the gel is improved, and the use of the antibiotics is reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of hydrogels, and specifically refers to an antibacterial and anti-inflammatory high-performance hydrogel and a preparation method thereof. Background Art

[0002] Existing wound dressings have relatively simple functions. Most wound dressings, such as band-aids, only have hemostasis and isolation functions and cannot further treat bacterial infections and inflammations at the wound. Although some antibiotic gel wound dressings have anti-infection effects, the widespread use of antibiotics will cause resistance selection of pathogenic bacteria; hydrogel is a high-water-content polymer material that forms a three-dimensional network structure through chemical or physical interactions. It has the advantages of excellent flexibility, good biocompatibility and simple preparation method; acetylcysteine ​​is an amino acid derivative and a powerful redox agent. Acetylcysteine ​​is widely used in medicine to treat various diseases and symptoms.

[0003] The existing technologies currently have the following main problems: 1. The widespread use of antibiotics causes drug resistance in pathogenic bacteria; 2. The wound dressings currently used have a single function. Summary of the invention

[0004] In view of the above situation, in order to overcome the defects of the prior art, the present invention provides an antibacterial and anti-inflammatory high-performance hydrogel and a preparation method thereof. In order to solve the problem of single function of wound dressing, the present invention proposes to combine acetylcysteine ​​with hydroxyethyl methacrylate to achieve the improvement of the antioxidant capacity of the drug-loaded gel, thereby reducing the inflammatory response. At the same time, acetylcysteine ​​and antibacterial sustained-release essential oils are used to replace antibiotics, thereby improving the antibacterial ability of the gel and reducing the use of antibiotics.

[0005] In order to achieve the above-mentioned purpose, the technical scheme adopted by the present invention is as follows: the present invention proposes an antibacterial and anti-inflammatory high-performance hydrogel and a preparation method thereof, wherein the antibacterial and anti-inflammatory high-performance hydrogel comprises the following components in parts by weight: 50-60 parts of high-performance gel and 6-8 parts of antibacterial sustained-release essential oil; the high-performance gel is obtained by cross-linking methacrylic acid-modified gelatin and hydroxyethyl methacrylate loaded with acetylcysteine; the antibacterial sustained-release essential oil is prepared from modified kaolinite and plant extracts.

[0006] Preferably, the high-performance gel is prepared from the following components in parts by weight: 80-120 parts of gelatin particles, 60-80 parts of glycidyl methacrylate, 90-100 parts of hydroxyethyl methacrylate, and 10-15 parts of acetylcysteine.

[0007] Preferably, the antibacterial slow-release essential oil is prepared from the following components in parts by weight: 10-15 parts of deep-sea giant kelp, 8-10 parts of crispa, 8-10 parts of eryngium maritimum, 5-6 parts of sea fennel, 15-20 parts of kaolinite, and 12-18 parts of lecithin.

[0008] Preferably, the method for preparing the high-performance gel comprises the following steps:

[0009] S1. Dissolve gelatin particles in deionized water to obtain a gelatin solution, add glycidyl methacrylate to the gelatin solution, and stir at 500 rpm for 24 h at 50-60°C to obtain a GelMA solution;

[0010] S2, dialyzing the GelMA solution obtained in S1 in a dialysis device, dialyzing against deionized water at 40-55°C, reverse dialyzing against polyethylene glycol-20000 at 40-55°C, and drying to obtain GelMA powder;

[0011] S3, mixing the photoinitiator 2959 aqueous solution and ethylene glycol in a volume ratio of 1:1 to obtain a mixed solution, adding the GelMA powder obtained in S2 to the mixed solution, and then adding hydroxyethyl methacrylate and acetylcysteine ​​to the mixed solution in sequence, heating and stirring at 50° C. until a complete solution is obtained to obtain a hydrogel preparatory solution;

[0012] S4. Expose the gel preparatory solution obtained in S3 under a light source, and then perform ultraviolet sterilization treatment to obtain a high-performance gel.

[0013] Preferably, in S1, the amount of gelatin particles added to deionized water is 35-60 mg / mL.

[0014] Preferably, in S2, the deionized water dialysis time is 40-180 hours, and the polyethylene glycol-20000 reverse dialysis time is 40-180 hours.

[0015] Preferably, in S3, the concentration of the photoinitiator 2959 aqueous solution is 1-2 wt %.

[0016] Preferably, in S3, the added amount of the GelMA powder is 0.1-0.2 g / mL.

[0017] Preferably, in S4, the wavelength of the light source is 300-400 nm, and the irradiation time is 30-100 s.

[0018] Preferably, the method for preparing the antibacterial slow-release essential oil specifically comprises the following steps:

[0019] (1) Washing deep-sea giant kelp, Cape cordata, Eryngium serratum, and sea fennel, drying them in an oven at 40° C., crushing them, and passing them through a 60-mesh sieve to obtain plant powder. Extracting the plant powder with a supercritical CO2 extraction device at an extraction pressure of 30-40 MPa, an extraction temperature of 40-60° C., and an extraction time of 60-100 min to obtain a composite essential oil;

[0020] (2) dispersing kaolinite in ethanol at a solid-liquid ratio of 1:20-25, adding lecithin, stirring at 8000-10000 rpm for 30-40 min at room temperature, centrifuging, washing, and drying to obtain modified kaolinite;

[0021] (3) dispersing the modified kaolinite obtained in step (3) in distilled water to obtain a 0.5-0.7 wt % kaolinite colloidal solution, mixing the kaolinite colloidal solution with the composite essential oil obtained in step (1) in a volume ratio of 1:0.2-0.3, and stirring at 6000 rpm for 30 min to obtain an antibacterial sustained-release essential oil.

[0022] The present invention also provides an antibacterial and anti-inflammatory high-performance hydrogel and a preparation method thereof, which specifically comprises the following steps:

[0023] The antibacterial slow-release essential oil is added to the high-performance gel, stirred at 80-100 rpm in a 40-50° C. water bath for 10-15 min, and mixed evenly to obtain an antibacterial and anti-inflammatory high-performance hydrogel.

[0024] The beneficial effects achieved by the present invention are as follows: both networks in the wound dressing can be cross-linked under ultraviolet light conditions to form a gel system; wherein NAC is loaded in the gel network through a chemical bond -SH to form a disulfide bond, and can be released in response to the oxidative stress state in the external environment, thereby alleviating the inflammatory response and having a broad-spectrum antibacterial effect, and can effectively kill common pathogens such as methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli (E. coli); the use of hydroxyethyl methacrylate enables the gel network to have good biocompatibility, can promote blood coagulation at the wound, absorb inflammatory exudate to promote wound healing, and enhance the mechanical properties of the gel network. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 The high performance gel technology roadmap of Example 1 of the present invention;

[0026] Figure 2 This is a real picture of the high performance gel of Example 1 of the present invention;

[0027] Figure 3 The anti-inflammatory performance test results of Examples 1-3 and Comparative Examples 2-3 of the present invention are shown in FIG.

[0028] Figure 4The figure is a graph showing the mechanical properties test results of Example 1 of the present invention and Comparative Example 1;

[0029] Figure 5 The graph is a test result of the antibacterial properties of Example 1 and Comparative Example 2 of the present invention.

[0030] The accompanying drawings are used to provide further understanding of the present invention and constitute a part of the specification. They are used to explain the present invention together with the embodiments of the present invention and do not constitute a limitation of the present invention. DETAILED DESCRIPTION

[0031] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments; based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0032] Unless otherwise defined, all professional and scientific terms used herein have the same meanings as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to those described herein may be applied to the present invention. The preferred implementation methods and materials described herein are for demonstration purposes only and are not intended to limit the content of this application.

[0033] The experimental methods in the following examples are conventional methods unless otherwise specified; the experimental materials used in the following examples are purchased from commercial channels unless otherwise specified.

[0034] Example 1

[0035] The invention discloses an antibacterial and anti-inflammatory high-performance hydrogel comprising the following components in parts by weight: 60 parts of high-performance gel and 8 parts of antibacterial slow-release essential oil.

[0036] The high-performance gel is prepared from the following components in parts by weight: 120 parts of gelatin particles, 80 parts of glycidyl methacrylate, 100 parts of hydroxyethyl methacrylate, and 15 parts of acetylcysteine.

[0037] The antibacterial slow-release essential oil is prepared from the following components in parts by weight: 15 parts of deep-sea giant kelp, 10 parts of crisp hornbeam, 10 parts of littoralis, 6 parts of sea fennel, 20 parts of kaolinite and 18 parts of lecithin.

[0038] The preparation method of high-performance gel specifically comprises the following steps:

[0039] S1. Dissolve gelatin particles in deionized water at an addition amount of 60 mg / mL to obtain a gelatin solution, add glycidyl methacrylate to the gelatin solution, and stir at 60° C. and 500 rpm for 24 h to obtain a GelMA solution;

[0040] S2, dialyzing the GelMA solution obtained in S1 in a dialysis device, dialyzing against deionized water at 55°C for 180 h, reverse dialyzing against polyethylene glycol-20000 at 55°C for 180 h, and drying to obtain GelMA powder;

[0041] S3, a 2 wt % photoinitiator 2959 aqueous solution and ethylene glycol were mixed in a volume ratio of 1:1 to obtain a mixed solution, the GelMA powder obtained in S2 was added to the mixed solution in an amount of 0.2 g / mL, and then hydroxyethyl methacrylate and acetylcysteine ​​were added to the mixed solution in sequence, and the mixture was heated and stirred at 50° C. until a complete solution was obtained to obtain a hydrogel preparatory solution;

[0042] S4. The gel preparatory solution obtained in S3 is irradiated under a 400 nm light source for 100 s, and then subjected to ultraviolet sterilization treatment to obtain a high-performance gel.

[0043] The preparation method of antibacterial slow-release essential oil specifically comprises the following steps:

[0044] (1) Washing deep-sea giant kelp, Cape crispus, Eryngium marinum and sea fennel, drying them in an oven at 40°C, crushing them, passing them through a 60-mesh sieve to obtain plant powder, and extracting the plant powder with a supercritical CO2 extraction device at an extraction pressure of 40 MPa, an extraction temperature of 60°C and an extraction time of 100 min to obtain a composite essential oil;

[0045] (2) dispersing kaolinite in ethanol at a solid-liquid ratio of 1:25, adding lecithin, stirring at 10000 rpm for 40 min at room temperature, centrifuging, washing, and drying to obtain modified kaolinite;

[0046] (3) Dispersing the modified kaolinite obtained in step (3) in distilled water to obtain a 0.7 wt % kaolinite colloidal solution, mixing the kaolinite colloidal solution with the composite essential oil obtained in step (1) in a volume ratio of 1:0.3, and stirring at 6000 rpm for 30 min to obtain an antibacterial sustained-release essential oil.

[0047] The present invention also provides an antibacterial and anti-inflammatory high-performance hydrogel and a preparation method thereof, which specifically comprises the following steps:

[0048] The antibacterial slow-release essential oil was added to the high-performance gel, stirred at 100 rpm for 15 min in a 50°C water bath, and mixed evenly to obtain an antibacterial and anti-inflammatory high-performance hydrogel.

[0049] Example 2

[0050] The invention discloses an antibacterial and anti-inflammatory high-performance hydrogel comprising the following components in parts by weight: 50 parts of high-performance gel and 6-8 parts of antibacterial slow-release essential oil.

[0051] The high-performance gel is prepared from the following components in parts by weight: 80 parts of gelatin particles, 60 parts of glycidyl methacrylate, 90 parts of hydroxyethyl methacrylate, and 10 parts of acetylcysteine.

[0052] The antibacterial slow-release essential oil is prepared from the following components in parts by weight: 10 parts of deep-sea giant kelp, 8 parts of crisp cape, 8 parts of eryngium maritima, 5 parts of sea fennel, 15 parts of kaolinite and 12 parts of lecithin.

[0053] The preparation method of high-performance gel specifically comprises the following steps:

[0054] S1. Dissolve gelatin particles in deionized water at an addition amount of 35 mg / mL to obtain a gelatin solution, add glycidyl methacrylate to the gelatin solution, and stir at 500 rpm for 24 h at 50° C. to obtain a GelMA solution;

[0055] S2, dialyzing the GelMA solution obtained in S1 in a dialysis device, dialyzing against deionized water at 40°C for 40 h, reverse dialyzing against polyethylene glycol-20000 at 40°C for 40 h, and drying to obtain GelMA powder;

[0056] S3, a 1 wt % photoinitiator 2959 aqueous solution and ethylene glycol were mixed in a volume ratio of 1:1 to obtain a mixed solution, the GelMA powder obtained in S2 was added to the mixed solution in an amount of 0.1 g / mL, and then hydroxyethyl methacrylate and acetylcysteine ​​were added to the mixed solution in sequence, and the mixture was heated and stirred at 50° C. until a complete solution was obtained to obtain a hydrogel preparatory solution;

[0057] S4. The gel preparatory solution obtained in S3 is irradiated under a 300 nm light source for 30 seconds, and then subjected to ultraviolet sterilization treatment to obtain a high-performance gel.

[0058] The preparation method of antibacterial slow-release essential oil specifically comprises the following steps:

[0059] (1) Washing deep-sea giant kelp, Cape crispus, Eryngium marinum and sea fennel, drying them in an oven at 40°C, crushing them, passing them through a 60-mesh sieve to obtain plant powder, and extracting the plant powder with a supercritical CO2 extraction device at an extraction pressure of 30 MPa, an extraction temperature of 40°C and an extraction time of 60 min to obtain a composite essential oil;

[0060] (2) dispersing kaolinite in ethanol at a solid-liquid ratio of 1:20, adding lecithin, stirring at 8000 rpm for 30 min at room temperature, centrifuging, washing, and drying to obtain modified kaolinite;

[0061] (3) Dispersing the modified kaolinite obtained in step (3) in distilled water to obtain a 0.5 wt % kaolinite colloidal solution, mixing the kaolinite colloidal solution with the composite essential oil obtained in step (1) in a volume ratio of 1:0.2, and stirring at 6000 rpm for 30 min to obtain an antibacterial sustained-release essential oil.

[0062] The present invention also provides an antibacterial and anti-inflammatory high-performance hydrogel and a preparation method thereof, which specifically comprises the following steps:

[0063] The antibacterial slow-release essential oil was added to the high-performance gel, stirred at 80 rpm for 10 min in a 40°C water bath, and mixed evenly to obtain an antibacterial and anti-inflammatory high-performance hydrogel.

[0064] Example 3

[0065] The invention discloses an antibacterial and anti-inflammatory high-performance hydrogel comprising the following components in parts by weight: 55 parts of high-performance gel and 7 parts of antibacterial slow-release essential oil.

[0066] The high-performance gel is prepared from the following components in parts by weight: 100 parts of gelatin particles, 70 parts of glycidyl methacrylate, 95 parts of hydroxyethyl methacrylate, and 12 parts of acetylcysteine.

[0067] The antibacterial slow-release essential oil is prepared from the following components in parts by weight: 12 parts of deep-sea giant kelp, 9 parts of crisp cape, 9 parts of littoralis, 5.5 parts of sea fennel, 18 parts of kaolinite and 15 parts of lecithin.

[0068] The preparation method of high-performance gel specifically comprises the following steps:

[0069] S1. Dissolve gelatin particles in deionized water at an addition amount of 40 mg / mL to obtain a gelatin solution, add glycidyl methacrylate to the gelatin solution, and stir at 500 rpm for 24 h at 55° C. to obtain a GelMA solution;

[0070] S2, dialyzing the GelMA solution obtained in S1 in a dialysis device, dialyzing against deionized water at 50°C for 60 h, reverse dialyzing against polyethylene glycol-20000 at 50°C for 60 h, and drying to obtain GelMA powder;

[0071] S3, a 1.5 wt% photoinitiator 2959 aqueous solution and ethylene glycol were mixed in a volume ratio of 1:1 to obtain a mixed solution, the GelMA powder obtained in S2 was added to the mixed solution in an amount of 0.15 g / mL, and then hydroxyethyl methacrylate and acetylcysteine ​​were added to the mixed solution in sequence, and the mixture was heated and stirred at 50° C. until a complete solution was obtained to obtain a hydrogel preparatory solution;

[0072] S4. The gel preparatory solution obtained in S3 is irradiated under a 350nm light source for 60s, and then subjected to ultraviolet sterilization treatment to obtain a high-performance gel.

[0073] The preparation method of antibacterial slow-release essential oil specifically comprises the following steps:

[0074] (1) Washing deep-sea giant kelp, Cape crispus, Eryngium marinum and sea fennel, drying them in an oven at 40°C, crushing them, passing them through a 60-mesh sieve to obtain plant powder, and extracting the plant powder with a supercritical CO2 extraction device at an extraction pressure of 35 MPa, an extraction temperature of 50°C and an extraction time of 80 min to obtain a composite essential oil;

[0075] (2) dispersing kaolinite in ethanol at a solid-liquid ratio of 1:22, adding lecithin, stirring at 9000 rpm for 30-40 min at room temperature, centrifuging, washing, and drying to obtain modified kaolinite;

[0076] (3) dispersing the modified kaolinite obtained in step (3) in distilled water to obtain a 0.5-0.7 wt % kaolinite colloidal solution, mixing the kaolinite colloidal solution with the composite essential oil obtained in step (1) in a volume ratio of 1:0.2-0.3, and stirring at 6000 rpm for 30 min to obtain an antibacterial sustained-release essential oil.

[0077] The present invention also provides an antibacterial and anti-inflammatory high-performance hydrogel and a preparation method thereof, which specifically comprises the following steps:

[0078] The antibacterial slow-release essential oil was added to the high-performance gel, stirred at 90 rpm for 12 min in a 45°C water bath, and mixed evenly to obtain an antibacterial and anti-inflammatory high-performance hydrogel.

[0079] Comparative Example 1

[0080] This comparative example provides a dressing, which is different from Example 1 only in that hydroxyethyl methacrylate (HEMA) is not included in the components, and the remaining components and component contents are the same as those in Example 1.

[0081] Comparative Example 2

[0082] This comparative example provides a dressing, which is different from Example 1 only in that the dressing does not contain acetylcysteine ​​(NAC), and the remaining components and component contents are the same as those in Example 1.

[0083] Experimental example

[0084] 1. Anti-inflammatory properties test

[0085] In the experiment, 8-week-old SD rats were selected and streptozotocin was intraperitoneally injected to prepare diabetic rats. The blank group consisted of 10 normal rats and the experimental group consisted of 40 diabetic rats. The rats in the experimental groups were randomly divided into 4 groups. The rats were anesthetized, the back hair was shaved, and a full-thickness skin wound with a diameter of 8 mm was created on the back with a perforator to prepare trauma rats. The experimental group was coated with Examples 1-3 and Comparative Example 2, and the blank group was not treated. The serum tumor necrosis factor-α (TNF-α) level of the rats was determined by chemiluminescence method at 1d and 7d, respectively.

[0086] 2. Mechanical properties test

[0087] The mechanical properties of Example 1 (GelMA-HEMA gel) and Comparative Example 1 (GelMA gel) were tested using an electronic universal testing machine. The hydrogel specimens for the compression test were cylindrical, the compression rate was 2 mm / min, and the compression stress-strain curve was plotted.

[0088] 3. Antibacterial performance test

[0089] The antibacterial experiment used Escherichia coli cells, and the hydrogel extracts of Example 1 (gel@NAC) and Comparative Example 2 (gel) were sterilized. The suspension of Escherichia coli was inoculated in an agar medium, and 1 mL of the extract of Example 1-2 was added as the experimental group, and 1 mL of normal saline was added as the control group. The cells were incubated at 37°C for 24 h, and then 1 mL of the culture solution was taken and cultured on an agar medium at 37°C for 30 h. Optical photographs were used to evaluate the antibacterial effect of the gel samples.

[0090] Figure 1 This is a technical roadmap for high-performance gel in Example 1 of the present invention; as shown in the figure, the preparation path of high-performance gel is clear, the preparation process is simple, and it is easy to produce.

[0091] Figure 2 This is a real picture of the high-performance gel of Example 1 of the present invention; as shown in the figure, the gel obtained after light cross-linking is solid and transparent.

[0092] Figure 3The anti-inflammatory performance test results of Examples 1-3 and Comparative Example 2 of the present invention are shown in the figure; as shown in the figure, the 1d serum tumor necrosis factor-α (TNF-α) levels of the blank group, Examples 1-3 and Comparative Example 2 are 2.36ng / mL, 4.02ng / mL, 4.04ng / mL, 4.03ng / mL, and 4.06ng / mL, respectively, and the 7d serum tumor necrosis factor-α (TNF-α) levels are 2.52ng / mL, 2.32ng / mL, 2.35ng / mL, 2.29ng / mL, and 3.25ng / mL, respectively; as shown in the figure, the serum tumor necrosis factor-α concentrations of Examples 1-3 at 7d are significantly decreased compared with those at 1d, the blank group is slightly increased, and the reduction in Comparative Example 2 is less than that of Examples 1-3, indicating that the use of acetylcysteine ​​improves the anti-inflammatory effect.

[0093] Figure 4 It is a graph showing the mechanical properties test results of Example 1 and Comparative Example 1 of the present invention; as shown in the figure, the compressive strength of Example 1 is significantly better than that of Comparative Example 1, and the addition of hydroxyethyl methacrylate increases the crosslinking density of the material and improves the mechanical properties of the gel material.

[0094] Figure 5 The figure is a graph showing the antibacterial performance test results of Example 1 and Comparative Example 2 of the present invention; as shown in the figure, the antibacterial effect of Example 1 is significantly better than that of Comparative Example 2, and the addition of acetylcysteine ​​improves the antibacterial performance of the gel material.

[0095] While the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that many changes, modifications, substitutions and variations can be made to the embodiments without departing from the principles and spirit of the invention.

[0096] The present invention and its implementation methods are described above, which is not restrictive. The drawings are only one of the implementation methods of the present invention, and the actual application is not limited thereto. In short, if ordinary technicians in the field are inspired by it and design methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention, they should all fall within the protection scope of the present invention.

Claims

1. A high-performance hydrogel with antibacterial and anti-inflammatory properties, characterized in that: The antibacterial and anti-inflammatory high-performance hydrogel comprises the following components in parts by weight: 50-60 parts of high-performance gel and 6-8 parts of antibacterial slow-release essential oil; the high-performance gel is obtained by cross-linking methacrylic acid-modified gelatin and hydroxyethyl methacrylate loaded with acetylcysteine; the antibacterial slow-release essential oil is prepared from modified kaolinite and plant extracts; The high-performance gel is prepared from the following components in parts by weight: 80-120 parts of gelatin particles, 60-80 parts of glycidyl methacrylate, 90-100 parts of hydroxyethyl methacrylate, and 10-15 parts of acetylcysteine; The antibacterial slow-release essential oil is prepared from the following components in parts by weight: 10-15 parts of deep-sea giant kelp, 8-10 parts of crispus, 8-10 parts of eryngium maritima, 5-6 parts of sea fennel, 15-20 parts of kaolinite and 12-18 parts of lecithin.

2. A method for preparing the antibacterial and anti-inflammatory high-performance hydrogel according to claim 1, characterized in that: The specific steps include: The antibacterial slow-release essential oil is added to the high-performance gel, stirred at 80-100 rpm in a 40-50° C. water bath for 10-15 min, and mixed evenly to obtain an antibacterial and anti-inflammatory high-performance hydrogel.

3. The method for preparing the antibacterial and anti-inflammatory high-performance hydrogel according to claim 2, characterized in that: The method for preparing the high-performance gel specifically comprises the following steps: S1. Dissolve gelatin particles in deionized water to obtain a gelatin solution, add glycidyl methacrylate to the gelatin solution, and stir at 500 rpm for 24 h at 50-60°C to obtain a GelMA solution; S2, dialyzing the GelMA solution obtained in S1 in a dialysis device, dialyzing against deionized water at 40-55°C, reverse dialyzing against polyethylene glycol-20000 at 40-55°C, and drying to obtain GelMA powder; S3, mixing the photoinitiator 2959 aqueous solution and ethylene glycol in a volume ratio of 1:1 to obtain a mixed solution, adding the GelMA powder obtained in S2 to the mixed solution, and then adding hydroxyethyl methacrylate and acetylcysteine ​​to the mixed solution in sequence, heating and stirring at 50° C. until a complete solution is obtained to obtain a hydrogel preparatory solution; S4. Expose the gel preparatory solution obtained in S3 under a light source, and then perform ultraviolet sterilization treatment to obtain a high-performance gel.

4. The method for preparing the antibacterial and anti-inflammatory high-performance hydrogel according to claim 3, characterized in that: The preparation method of the antibacterial slow-release essential oil specifically comprises the following steps: (1) Washing deep-sea giant kelp, Cape cordata, Eryngium serratum, and sea fennel, drying them in an oven at 40° C., crushing them, and passing them through a 60-mesh sieve to obtain plant powder. Extracting the plant powder with a supercritical CO2 extraction device at an extraction pressure of 30-40 MPa, an extraction temperature of 40-60° C., and an extraction time of 60-100 min to obtain a composite essential oil; (2) dispersing kaolinite in ethanol at a solid-liquid ratio of 1:20-25, adding lecithin, stirring at 8000-10000 rpm for 30-40 min at room temperature, centrifuging, washing, and drying to obtain modified kaolinite; (3) dispersing the modified kaolinite obtained in step (3) in distilled water to obtain a 0.5-0.7 wt % kaolinite colloidal solution, mixing the kaolinite colloidal solution with the composite essential oil obtained in step (1) in a volume ratio of 1:0.2-0.3, and stirring at 6000 rpm for 30 min to obtain an antibacterial sustained-release essential oil.

5. The method for preparing the antibacterial and anti-inflammatory high-performance hydrogel according to claim 4, characterized in that: In S1, the gelatin particles are added in an amount of 35-60 mg / mL in deionized water.

6. The method for preparing the antibacterial and anti-inflammatory high-performance hydrogel according to claim 5, characterized in that: In S2, the deionized water dialysis time is 40-180 hours; the polyethylene glycol-20000 reverse dialysis time is 40-180 hours.

7. The method for preparing the antibacterial and anti-inflammatory high-performance hydrogel according to claim 6, characterized in that: In S3, the concentration of the photoinitiator I-2959 aqueous solution is 1-2wt%; and the added amount of the GelMA powder is 0.1-0.2g / mL.

8. The method for preparing the antibacterial and anti-inflammatory high-performance hydrogel according to claim 7, characterized in that: In S4, the wavelength of the light source is 300-400 nm, and the irradiation time is 30-100 s.

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