Composition for preventing and treating crop wilt and application

Abstract

The invention belongs to the field of research and application of melon commercial crops, and particularly relates to a composition for preventing and treating crop wilt and application. The composition for preventing and treating the crop fusarium wilt comprises 4-hydroxystyrene and kaempferol, cucumber plants treated by the composition can survive and continue to grow after fusarium wilt inoculation, and the composition plays a positive role in preventing the crop fusarium wilt.

Description

Technical Field

[0001] The invention belongs to the research and application field of melon economic crops, and particularly relates to a composition for preventing and treating crop wilt disease and an application thereof. Background Art

[0002] Cucumber wilt is a fungal soil-borne disease caused by Fusarium oxysporum, which has a serious impact on cash crops worldwide, especially on cucurbit crops such as cucumber, watermelon, and melon. This disease not only causes significant economic losses, but also becomes a major challenge in agricultural production due to its difficult-to-control characteristics.

[0003] At present, the methods for preventing and controlling Fusarium wilt mainly include: applying chemical agents to treat the soil, grafting resistant rootstocks and cultivating resistant varieties. Among them, the use of chemical agents and grafting will pollute the environment and increase the workload. Although the breeding of resistant varieties has a stronger disease resistance effect, the current resistant strains are relatively single and it is difficult to have the excellent traits of other strains at the same time. Therefore, the existing methods for preventing and controlling Fusarium wilt have certain limitations.

[0004] In summary, providing a composition for preventing cucumber wilt disease with a wide range of applications, a simple method of use, and good prevention and control effects has become an urgent problem to be solved in the art. Summary of the invention

[0005] The present invention provides a composition for preventing and treating crop wilt and its application, which specifically includes the following technical solutions:

[0006] A composition comprising the following raw materials in parts by weight:

[0007] 1-3 parts of 4-hydroxystyrene, 1-3 parts of kaempferol and 2×10 7 share.

[0008] Preferably, the composition comprises the following raw materials in parts by weight:

[0009] 1 part of 4-hydroxystyrene, 1 part of kaempferol and 2×10 7 share.

[0010] Preferably, the final concentration of the 4-hydroxystyrene in the composition is 0.05 mg / L to 0.15 mg / L; the final concentration of the kaempferol in the composition is 0.05 mg / L to 0.15 mg / L.

[0011] Preferably, the solvent comprises sterile water.

[0012] The present invention also provides use of the composition as described in any one of the above items in preventing and controlling crop wilt.

[0013] Preferably, the crop blight disease includes Cucurbitaceae crop blight disease.

[0014] The present invention also provides a method for preventing and controlling crop wilt, comprising the following steps:

[0015] The composition according to any one of claims 1 to 4 is sprayed on the leaves of crops at the seedling stage until the leaf surface is wetted and droplets fall.

[0016] Preferably, the seedling stage includes the two-leaf and one-heart stage.

[0017] Preferably, the pathogen of the wilt disease is Fusarium oxysporum f. sp. Cucumerinum.

[0018] Preferably, the crop wilt disease includes cucumber wilt.

[0019] The beneficial effects of the present invention are:

[0020] The present invention provides a composition, comprising the following raw materials in parts by weight: 1 to 3 parts of 4-hydroxystyrene, 1 to 3 parts of kaempferol and 2×10 7 The present invention selects 4-hydroxystyrene and kaempferol as active ingredients, and the two can be synthesized by the plant itself. Therefore, the use of the composition of the present invention to control crop wilt has the advantages of being environmentally friendly and pollution-free compared with chemical agents for controlling crop wilt; and the two metabolites can play a role in wilt resistance, and can effectively prevent and slow down the onset of cucumber wilt. The plants treated with the mixture of 4-hydroxystyrene and kaempferol had a significantly improved survival rate after wilt inoculation, had good growth conditions, and had enhanced resistance to diseases.

[0021] The composition of the present invention effectively reduces the use of chemical pesticides and reduces pollution to the environment. Compared with the methods of breeding disease-resistant varieties, using pesticides, soil disinfection and grafting, which have the defects of pollution, poor effect, complicated operation, long cycle, etc., the composition of the present invention can effectively reduce the use of chemical pesticides and reduce pollution to the environment. At the same time, the application method is simple and can quickly improve the disease resistance of plants, which has obvious advantages. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments are briefly introduced below.

[0023] Figure 1 This is a schematic diagram of the disease conditions of cucumber seedlings in different treatment groups in Example 6 of the present invention;

[0024] Among them, (A) is a control plant with normal growth; (B) is an infected plant inoculated with wilt bacteria; (C) is an infected plant inoculated with wilt bacteria and then applied with a mixture of 4-hydroxystyrene and kaempferol; (D) is an infected plant inoculated with wilt bacteria and then applied with 4-hydroxystyrene alone; (E) is an infected plant inoculated with wilt bacteria and then applied with kaempferol alone. DETAILED DESCRIPTION

[0025] The present invention provides a composition comprising the following raw materials in parts by weight:

[0026] 1-3 parts of 4-hydroxystyrene, 1-3 parts of kaempferol and 2×10 7 share.

[0027] In terms of parts by weight, as an embodiment, the parts by weight of 4-hydroxystyrene in the composition of the present invention can be 1 part, 2 parts or 3 parts; as an embodiment, the final concentration of the 4-hydroxystyrene in the composition is 0.05 mg / L to 0.15 mg / L; as an embodiment, the final concentration of the 4-hydroxystyrene in the composition can be 0.05 mg / L, 0.1 mg / L or 0.15 mg / L.

[0028] In terms of parts by weight, as an embodiment, the parts by weight of kaempferol in the composition of the present invention can be 1 part, 2 parts or 3 parts; as an embodiment, the final concentration of kaempferol in the composition is 0.05 mg / L to 0.15 mg / L; as an embodiment, the final concentration of kaempferol in the composition can be 0.05 mg / L, 0.1 mg / L or 0.15 mg / L.

[0029] In parts by weight, as an embodiment, the parts by weight of the solvent may be 2×10 7 As one embodiment, the solvent is sterile water; as another embodiment, the sterile water is sterile distilled water.

[0030] In a specific embodiment, the composition of the present invention comprises the following raw materials in parts by weight: 1 part of 4-hydroxystyrene, 1 part of kaempferol and 2×10 7 share.

[0031] The present invention also provides application of the above composition in preventing and controlling crop wilt.

[0032] As an embodiment, the crop wilt disease described in the present invention includes Cucurbitaceae crop wilt disease; as an embodiment, the pathogen of the crop wilt disease includes Fusarium oxysporum f. sp. Cucumerinum; as an embodiment, the Cucurbitaceae crop includes any one of cucumber, watermelon, and melon; in a specific embodiment, the crop wilt disease is cucumber wilt disease.

[0033] The present invention also provides a method for preventing and controlling crop wilt, comprising the following steps:

[0034] The above composition is sprayed at the seedling stage on the leaves of the crop until the leaf surface is wetted and droplets fall.

[0035] As an embodiment, the method for preventing and controlling crop wilt disease also includes spraying the composition once every 2 to 3 days during the seedling stage until the seedling stage is passed; as an embodiment, the frequency of spraying the composition can be spraying once every 2 days or once every 3 days; as an embodiment, the seedling stage of the present invention includes the two-leaf and one-heart stage, and the characteristics of the crop and wilt disease are the same as the preferred method in the above-mentioned application and are not limited here.

[0036] In order to further illustrate the present invention, the composition and application thereof provided by the present invention are described in detail below in conjunction with the accompanying drawings and examples, but they should not be construed as limiting the scope of protection of the present invention.

[0037] Example 1 A composition for preventing and treating crop wilt

[0038] The weight proportions of the raw materials of the composition are as follows: 1 part of 4-hydroxystyrene, 1 part of kaempferol, 2×10 7 share.

[0039] Example 2 A composition for preventing and treating crop wilt

[0040] The weight proportions of the raw materials of the composition are as follows: 2 parts of 4-hydroxystyrene, 2 parts of kaempferol, 2×10 7 share.

[0041] Example 3 A composition for preventing and treating crop wilt

[0042] The weight proportions of the raw materials of the composition are as follows: 3 parts of 4-hydroxystyrene, 3 parts of kaempferol, 2×10 7 share.

[0043] Example 4 Preparation method of a composition for preventing and treating crop wilt

[0044] This example uses the raw materials shown in Example 1 to prepare the composition, and the preparation method is as follows:

[0045] Weigh 1 part of 4-hydroxystyrene and 1 part of kaempferol, use sterile water as a solvent, prepare 4-hydroxystyrene and kaempferol into stock solutions with a concentration of 1 mg / L respectively with sterile water, and then dilute them tenfold to obtain a 4-hydroxystyrene solution with a concentration of 0.1 mg / L and a kaempferol solution with a concentration of 0.1 mg / L;

[0046] The 4-hydroxystyrene solution and the kaempferol solution are mixed in a ratio of 1:1 to obtain a composition for preventing and controlling crop wilt.

[0047] Example 5 A method for preventing and controlling crop wilt

[0048] In this example, the composition prepared by the preparation method described in Example 4 is used to prevent and treat crop wilt, and the steps are as follows:

[0049] Use a spray bottle to evenly spray the mixture onto the leaves of the cucumber seedlings until the mixture soaks the leaves and begins to drip; the spraying period is the two-leaf and one-heart stage of the cucumber seedlings.

[0050] If the cucumber plants have already developed wilt, spray the composition prepared by the preparation method described in Example 4 once every 2 days, and spray each time until the mixture soaks the leaves and starts to drip.

[0051] Example 6 Effect verification of the composition for preventing and controlling cucumber wilt

[0052] Select 150 cucumber seeds with full seeds and uniform size, soak them in 1% sodium hypochlorite solution for 10 minutes for disinfection, rinse them with sterilized distilled water 3 to 4 times, and finally absorb excess water on filter paper. Spread the prepared cucumber seeds in a hydroponic container and place them in a dark environment at 28°C for germination. After the cucumber seeds germinate, select 100 cucumber buds with uniform growth and transplant them into a small pot with a diameter of 12 cm and a height of 9 cm for cultivation. The substrate in the small pot is a mixture of nutrient soil, peat, and vermiculite in a volume ratio of 1:1:1. The effect is verified when the seedlings grow to two leaves and one heart.

[0053] Preparation of pathogen spore suspension: The slant tube of Fusarium wilt pathogen was provided by the laboratory of College of Landscape and Horticulture of Yunnan Agricultural University. It was a cucumber-specific strain of Fusarium oxysporum. The strains stored in the slant tube culture medium were taken out from the 4°C refrigerator and cultured in PD culture medium. The shaking culture conditions were 28°C, 160r / min, and cultured for 5 days. The bacterial solution was filtered through 4 layers of gauze and diluted with sterile water to prepare a spore suspension. 1×10 8 The spore suspension with a concentration of spores / ml is ready for use.

[0054] PD medium was prepared from the following ingredients: 200 g potato, 20 g glucose, and 1 L water.

[0055] The cucumber seedlings were divided into five groups and treated with the blank control group, the control group and the experimental examples 1 to 3 groups respectively. The spraying was performed once every 2 days. Each group was set up with 20 replicates. After six days of treatment, the growth of the plants in each group was observed. The results are shown in Table 1 and Figure 1 shown.

[0056] (1) Blank control group: Starting from the two-leaf and one-heart stage, cucumber seedlings were sprayed with clean water every day;

[0057] (2) Control group: At the two-leaf-one-heart stage, cucumber plants were infected with the pathogen spore suspension as described above by using root wounding supplemented with root dipping inoculation method;

[0058] (3) Experimental Example 1: At the two-leaf and one-heart stage, the root injury supplemented with root immersion inoculation method was used. The above-mentioned pathogen spore suspension was used to supplement the root injury supplemented with root immersion inoculation method: the roots of the seedlings were rinsed with clean water, and 1 / 2 of the fibrous roots were cut off with scissors without damaging the main root for root injury treatment. The roots were moved to the spore suspension and soaked for 1 hour before being planted in black plastic nutrient pots. Each plant in the treatment group was irrigated with 20 mL of bacterial solution, and each plant in the control group was irrigated with 20 mL of sterile water. The cultivation was continued under the room temperature greenhouse, and the temperature was basically maintained at 24-28°C during the day and 14-18°C at night. Infect cucumber plants. After infection, spray the composition described in Example 1 to prevent and control cucumber wilt. The spraying method is to spray the composition on the leaves of the cucumber seedlings until the mixture soaks the leaves and begins to drip the liquid;

[0059] (4) Experimental Example 2: The operation was the same as that of Experimental Example 1, except that only 0.1 mg / L 4-hydroxystyrene solution was used to control cucumber wilt, and the spraying method was to spray the composition on the leaves of cucumber seedlings until the mixture soaked the leaves and began to drip the liquid;

[0060] (5) Experimental Example 3: The operation was the same as that of Experimental Example 1, except that only 0.1 mg / L kaempferol solution was used to control cucumber wilt. The spraying method was to spray the composition on the leaves of cucumber seedlings until the mixture soaked the leaves and began to drip the liquid.

[0061] Table 1 The incidence of cucumber seedlings in different treatment groups

[0062] Number of plants treated Number of dead plants Survival rate (%) Blank control group 20 0 100 Control group 20 20 0 Experimental Example 1 20 16 80 Experimental Example 2 20 10 50 Experimental Example 3 20 12 60

[0063] As shown in Table 1, the application of a mixture of 4-hydroxystyrene and kaempferol can significantly improve the survival rate of cucumber plants with Fusarium wilt, while the survival rate of cucumber plants with Fusarium wilt is only 50-60% when 4-hydroxystyrene or kaempferol is applied alone. This shows that there is a synergistic effect between 4-hydroxystyrene and kaempferol. Figure 1 It can be seen that Figure 1 The group (A) is the blank control group, and the cucumber seedlings in this group can grow normally; Figure 1 (B) The cucumber seedlings in the control group inoculated with Fusarium wilt will have yellowing leaves and wilting. Figure 1 In (C), the plants treated with a mixture of 4-hydroxystyrene and kaempferol grew better and did not show severe disease symptoms. Figure 1 (D) 4-hydroxystyrene alone or Figure 1 In the middle (E), the anti-disease effect of the group that applied kaempferol alone was significantly weaker than that of the combination of the two.

[0064] In summary, the composition of the present invention can effectively prevent and slow down the onset of cucumber wilt disease. The survival rate of plants treated with the mixture of 4-hydroxystyrene and kaempferol is significantly improved after wilt disease inoculation, the growth condition is good, and the resistance to the disease is enhanced.

[0065] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments like this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A composition, characterized in that The invention comprises the following raw materials in parts by weight: 1-3 parts of 4-hydroxystyrene, 1-3 parts of kaempferol and 2×10 7 share.

2. The composition according to claim 1, characterized in that The invention comprises the following raw materials in parts by weight: 1 part of 4-hydroxystyrene, 1 part of kaempferol and 2×10 7 share.

3. The composition according to claim 1, characterized in that The final concentration of the 4-hydroxystyrene in the composition is 0.05 mg / L to 0.15 mg / L; the final concentration of the kaempferol in the composition is 0.05 mg / L to 0.15 mg / L.

4. The composition according to claim 1, characterized in that The solvent includes sterile water.

5. Use of the composition according to any one of claims 1 to 4 in preventing and controlling crop wilt.

6. The use according to claim 5, characterized in that The crop blight disease includes the cucurbitaceae crop blight disease.

7. A method for preventing and controlling crop wilt, characterized in that: The steps include: The composition according to any one of claims 1 to 4 is sprayed on the leaves of crops at the seedling stage until the leaf surface is wetted and droplets fall.

8. The method according to claim 7, characterized in that The seedling stage includes the two-leaf and one-heart stage.

9. The method according to claim 7, characterized in that The pathogen of the wilt disease is Fusarium oxysporumf.sp.Cucumerinum.

10. The method according to claim 7 or 9, characterized in that The crop wilt disease includes cucumber wilt.

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