Red seed composition with whitening effect and application of red seed composition in inhibition of tyrosinase

Abstract

The invention relates to the technical field of skin care and whitening, and discloses a red seed composition with a whitening effect and application of the red seed composition to inhibition of tyrosinase, and the red seed composition is prepared from, by weight, 15-25 parts of humulus lupulus extract, 10-15 parts of red seed extract and 10-15 parts of licorice overground part extract. The weight part of the red seed extract is 10-25 parts, the weight part of the licorice overground stem and leaf part extract is 5-15 parts, and the weight part of the deionized water is 40-60 parts. Through scientific proportioning of the humulus lupulus extract, the red seed extract and the liquorice overground stem and leaf part extract, the effects of all the components can be fully exerted, and all the components cooperate with one another to achieve a synergistic interaction effect; the composition plays roles in four aspects of improving skin health circulation, removing free radicals in the body, improving chromatosis and moisturizing and moistening the skin, so that the effect of brightening the skin can be achieved more lastingly and effectively.

Description

Technical Field

[0001] The invention relates to the technical field of skin care and whitening, and in particular to a red seed composition with whitening effect and its application in inhibiting tyrosinase. Background Art

[0002] Melanin is a heterogeneous polyphenolic biopolymer secreted by melanocytes in the basal layer of the skin. The type, quantity and distribution of melanin determine the skin color. Melanin is generated through a series of reactions: tyrosine is oxidized to form dopa under the catalysis of tyrosinase, then dopaquinone is formed, and then dopachrome, as well as dihydroxyindole and ketoindole are generated, and finally melanin is polymerized; therefore, the more tyrosinase content, the higher the activity, the easier it is to form melanin, and melanin moves through layers and transfers to the epidermis of the skin, thus forming freckles, dark spots, sun spots, etc.

[0003] Therefore, tyrosinase (EC.1.14.18.1) is a copper-containing enzyme widely present in nature and is a key enzyme in the biosynthesis of melanin. The production of melanin is inhibited by inhibiting the activity of tyrosinase. For this reason, tyrosinase inhibitors can be used clinically to treat certain skin diseases related to excessive melanin deposition and have a whitening effect in cosmetics.

[0004] At present, tyrosinase inhibitors can be roughly divided into two categories: chemical synthesis inhibitors and natural extract inhibitors. Chemical synthesis inhibitors mainly include hydroquinone and azelaic acid. Long-term use will have adverse effects on the human body. For example: long-term use of hydroquinone not only has great side effects on the human body, but also causes melanin deposition in the skin, making it difficult to remove. Azelaic acid will irritate the skin, eyes, mucous membranes and upper respiratory tract.

[0005] Natural extract inhibitors mainly include kojic acid, arbutin, glycyrrhizin and safflower flavonoids. Although they are harmless to the human body, their sources are limited and their prices are high. For example, natural arbutin only exists in bearberry leaves. Not only are bearberries rare and difficult to grow, but the content of arbutin in their leaves is also low. In addition, kojic acid is unstable and easily oxidized, and has a certain irritation. In recent years, some studies have shown that kojic acid has a certain carcinogenicity, so it has been less used. Arbutin is similar to hydroquinone in structure, and it is easily hydrolyzed to produce hydroquinone and D-glucose under weakly acidic conditions. Therefore, it is speculated that arbutin entering the human body may be hydrolyzed to produce hydroquinone under acidic conditions. Therefore, in recent years, some scholars have also questioned the safety of arbutin.

[0006] Therefore, the current research direction is glycyrrhizin and safflower flavonoids. For example, tyrosinase inhibitors are obtained by extracting flavonoids from peony, rose, and rose. However, the flavonoids extracted from peony, rose, and rose have poor solubility (especially poor fat solubility). Since the human cell wall is composed of lipid components, the use of poorly fat-soluble tyrosinase inhibitors is not conducive to skin absorption and has a relatively low bioavailability. At the same time, the whitening effect is greatly weakened, which also limits the scope of use of tyrosinase inhibitors. Summary of the invention

[0007] The present invention aims to provide a red seed composition with whitening effect and its application in tyrosinase inhibition. The inventor conducts research and development in the direction of total flavonoids, and solves the technical problem of poor fat solubility of brass components by isolating and passivating the effective brass components in the extraction composition.

[0008] In order to achieve the above object, the present invention adopts the following technical scheme:

[0009] 1) A red seed composition with whitening effect, comprising hops extract, red seed extract and licorice aerial part extract, wherein the hops extract is 15-25 parts by weight, the red seed extract is 10-25 parts by weight, the licorice aerial stem and leaf part extract is 5-15 parts by weight, and deionized water is 40-60 parts by weight.

[0010] The active ingredients of the hop extract of the present invention are α-acid, extract (α-acid, β-acid) and polyphenols. It can inhibit the action of lipoxygenase and protect "oxidation-sensitive substances" such as lipids from being rapidly oxidized by enzymes. At the same time, it can also be used as a chelating agent to isolate the transfer reaction of heavy metal ions (such as copper and iron metal ions), which is equivalent to eliminating the catalytic effect of metals and preventing the generation of a large number of oxygen free radicals. Therefore, the hop extract is added to the red seed composition, which can not only play an antioxidant role, but also prolong the shelf life of the cosmetics made from the red seed composition.

[0011] The red seeds of the present invention are the seeds of Pyracantha fortuneana, which can eliminate stagnation and stop dysentery, and promote blood circulation and stop bleeding. They are used for indigestion, enteritis, dysentery, infantile malnutrition, metrorrhagia, leucorrhea, and abdominal pain after childbirth. The organic acid components in the red seeds can inhibit the activity of tyrosinase, promote the renewal of epidermal keratinocytes, and accelerate melanin metabolism; the flavonoid components have the functions of scavenging free radicals and inhibiting tyrosinase, and thus have the effects of whitening and delaying aging.

[0012] The aboveground stem and leaf part of the licorice of the present invention is rich in resources and has a high content of flavonoid components. Studies have found that the content of the aboveground stem and leaf part of the licorice in full bloom is about 5.64%, and at the same time, it has pharmacological effects such as antibacterial and antioxidant. In addition, the aboveground stem and leaf part extract precipitate of the licorice contains more fat-soluble flavonoid components, mainly isoflavones, dihydroflavones, flavonol flavonoid aglycone substances, and the flavonol flavonoid aglycone substance components have multiple pharmacological activities such as anti-oxidation, anti-inflammatory, and antibacterial, and the aglycone substances are directly absorbed in human blood, and the potency thereof is 7 times that of the glycoside substances.

[0013] Therefore, the present invention, through the scientific ratio of the components of hops extract, red seed extract, and licorice aboveground stem and leaf extract, can not only fully exert the efficacy of each component, but also the components cooperate with each other to play a synergistic role, and play a role in four aspects: improving skin healthy circulation, removing free radicals in the body, improving pigmentation, and moisturizing the skin, thereby achieving a more lasting and effective effect of brightening the skin.

[0014] 2) A red seed composition with whitening effect according to 1), wherein:

[0015] The hop extract is 20 parts by weight, the red seed extract is 25 parts by weight, the licorice aerial stem and leaf extract is 15 parts by weight, and the deionized water is 40 parts by weight.

[0016] The hop extract, red seed extract and licorice aerial stem and leaf extract are scientifically proportioned in the above weight components. The red seed extract and the hop extract respectively have the effect of inhibiting tyrosinase (EC.1.14.18.1), and the two have a synergistic effect to increase cell activity, have anti-photoaging effects, and have obvious anti-radiation effects. By mixing the licorice aerial stem and leaf extract with the hop extract and the red seed extract, the composition can be effectively absorbed by the skin, and the bioavailability is greatly increased.

[0017] 3) A red seed composition with whitening effect according to 1), wherein:

[0018] The hop extract is 15 parts by weight, the red seed extract is 20 parts by weight, the licorice aerial stem and leaf extract is 10 parts by weight, and the deionized water is 55 parts by weight.

[0019] The hop extract, red seed extract and licorice aerial stem and leaf extract are scientifically proportioned according to the above weight components, and the red seed extract is used as a guide to connect the hop extract and the licorice aerial stem and leaf extract. The organic acid component of the red seed extract cooperates with the total flavonoids in the hop extract and is integrated with the fat-soluble flavonoid components in the licorice aerial stem and leaf extract, thereby solving the technical problem of poor fat solubility of the flavonoid components. When applied to tyrosinase inhibitors, it is beneficial to the absorption of human cell walls, the bioavailability is greatly improved, and the scope of use of the tyrosinase inhibitor is increased.

[0020] 4) A red seed composition with whitening effect according to 1), wherein:

[0021] The hop extract is 25 parts by weight, the red seed extract is 10 parts by weight, the licorice aerial stem and leaf extract is 5 parts by weight, and the deionized water is 60 parts by weight.

[0022] In the present invention, the weight component of the hop extract is greater than the weight component of the red seed extract, the weight component of the red seed extract is twice the weight component of the extract of the aerial stem and leaf part of licorice, and at the same time, the weight component of the hop extract is three times the weight component of the aerial stem and leaf part of licorice. With this design, a synergistic effect can be formed between the hop extract and the red seed extract based on the aerial stem and leaf part of licorice, the skin barrier can be repaired by improving the water retention capacity of the skin epidermis and reducing the transepidermal water loss value, and the whitening effect is achieved by inhibiting tyrosinase activity and melanin synthesis; in addition, the activity of hyaluronidase is inhibited, thereby reducing the content of inflammatory factors.

[0023] The composition of the present invention can achieve the dual effects of repair and whitening from multiple dimensions such as increasing the water content of the stratum corneum, strengthening the skin barrier, repairing inflammatory reactions, and inhibiting the activities of hyaluronidase and tyrosinase. During the entire skin renewal process, it plays a targeted regulatory role on dry, sensitive and dull skin.

[0024] 5) A red seed composition with whitening effect according to any one of 1) to 4), wherein the step of extracting the hop extract comprises:

[0025] Step 1, subjecting the cleaned hops to hot steam treatment, and then quickly placing the hops in a freezer for freezing treatment to obtain solid hops, wherein the water content of the hops is 15%-20%;

[0026] Step 2, the solid hops in step 2 are pounded into juice and ethanol solution is added to the juice, the juice and the ethanol solution are fully stirred at room temperature, and a mixed liquid is obtained after standing for 1-3 hours, the mixed liquid is subjected to vibration extraction and filtration, and ethanol is recovered from the filtrate to obtain hop liquid;

[0027] Step 3: placing the hop liquid in step 3 in a freezer for freeze drying to obtain frozen hop powder, and sieving the frozen hop powder with 100-130 mesh to obtain hop extract.

[0028] In the first step of the present invention, the surface of hops is heated by hot steam, which can passivate peroxidase, polyphenol oxidase and the like contained in the hops, thereby preventing the hop extract from containing too much peroxidase and polyphenol magnesium oxide, and preventing the skin from browning due to excessive peroxidase and polyphenol magnesium oxide, thereby effectively controlling the substances contained in the hops; at the same time, the hops heated by hot steam are quickly placed in a freezer for freeze drying, so that the water in the hops is sublimated and various material components in the hops are maintained.

[0029] The freeze-dried hops are pounded into juice and fully stirred with an ethanol solution to obtain a mixed liquid, and the mixed liquid is extracted and filtered with the assistance of an ethanol solution to obtain a hop liquid, and then the hop liquid is freeze-dried in a freezer to obtain a hop extract. The hop extract obtained in this way can maintain its internal material components after two freeze-dryings, can quickly improve the extraction of material components such as hop polyphenols, can isolate the transfer reaction of heavy metal ions, prevent the generation of a large number of oxygen free radicals, and play an antioxidant role.

[0030] 6) A red seed composition with whitening effect according to 5), wherein:

[0031] In step 2, the juice and the ethanol solution are fully stirred and mixed and then allowed to stand to obtain a mixed liquid, and the mixed liquid is subjected to ultrasonic-assisted extraction of the hop liquid with the ethanol solution.

[0032] The amount of ethanol solution used in the present invention is 15-20 times the total mass of solid hops. At the same time, in the process of extracting hop liquid with ultrasonic-assisted ethanol solution, the extraction temperature is 50-60°C, the time is 15-30min, and the ultrasonic frequency is 60KHz, so that the hops can be quickly extracted while maintaining the effective substance components in the hops.

[0033] 7) A red seed composition with whitening effect according to any one of 1) to 4), wherein the step of extracting the red seed extract comprises:

[0034] Step 1: Place the cleaned red seeds in a freezer for freeze drying, so that the water content of the red seeds is 0.5-1%, and crush and sieve the freeze-dried red seeds to make 30-60 mesh red seed powder;

[0035] Step 2: preparing an extract with deionized water and ethanol solution in a ratio of 3:6, and soaking the red seed powder in the extract for 48-62 hours to obtain a red seed immersion solution;

[0036] Step 3: reflux the red seed immersion solution in step 2 for 30 minutes to obtain a red seed filtrate, and evaporate the liquid of the red seed filtrate through a rotary evaporator to obtain a red seed extract.

[0037] 8) A red seed composition with whitening effect according to 7), wherein:

[0038] In step three, the reflux extraction includes several cooling extraction sections and heating extraction sections, which are arranged alternately to form intermittent reflux extraction, wherein the extraction is stopped between the cooling extraction sections and the heating extraction sections, and then the temperature is increased for extraction once after cooling to room temperature, and the extraction temperature does not exceed 80°C. After extraction, the red seed filtrate is obtained by vacuum filtration.

[0039] 9) A red seed composition with whitening effect according to any one of 1) to 4), wherein the step of extracting the aboveground stems and leaves of licorice comprises:

[0040] Step 1, washing the rhizomes and leaves on the aboveground stems and leaves of licorice, and then steam drying the rhizomes and leaves. After the steam drying treatment, the rhizomes and leaves are quickly placed in a freezer for freeze drying, so that the water content in the rhizomes and leaves is 5-10%;

[0041] Step 2: Mix and crush the rhizomes and leaves obtained by freeze-drying in step 1 to form a mixture slurry, add ethanol solution to the mixture slurry, and extract the mixed extract by ultrasonic-assisted extraction;

[0042] Step 3: Filter the mixed extract to obtain a mixture precipitate, and place the mixture precipitate in a freezer for freeze-drying to obtain an extract of the aboveground stems and leaves of licorice.

[0043] 10) A red seed composition with whitening effect for use in inhibiting tyrosinase, comprising: preparing a skin care cosmetic or medicine by a preparation method of the red seed composition with whitening effect described in any one of 1) to 9), wherein the preparation method comprises the following steps:

[0044] Step 1: Red seed composition: based on weight components, take 15-25 parts of hops, 10-25 parts of red seed extract, and 5-15 parts of licorice aerial stem and leaf extract;

[0045] Step 2: Taking 40-60 parts of deionized water by weight, heating the deionized water to 50-70° C., during the heating of the deionized water, adding the red seed composition in step 1 into the deionized water, stirring for 20 minutes, and then standing for separation and passivation to obtain a red seed composition mixed solution, and vacuum concentrating the red seed composition mixed solution to obtain a red seed solid;

[0046] Step 3: Take 20-30 parts of deionized water by weight, and add 0.5-1% of fenugreek oil to the deionized water to obtain an oil phase raw material, slowly add the red seed solid matter in step 2 to the oil phase raw material, so that the red seed solid matter is fully emulsified in the oil phase raw material, continue to slowly stir, and cool to 35-45° C.

[0047] The vacuum concentration in the present invention includes a high-temperature vacuum concentration stage and a low-temperature vacuum concentration stage, wherein the high-temperature vacuum concentration stage adopts a temperature of 75-80°C, a vacuum degree of 0.03-0.07MPa, an evaporation rate controlled at 50-60mL / min, and a time of 20-30min; the low-temperature vacuum concentration adopts a temperature of 35-40°C, a vacuum degree of 0.05-0.09MPa, an evaporation rate controlled at 15-25mL / min, and a time of 30-50min; after the high-temperature vacuum concentration stage and the low-temperature vacuum concentration stage, the vacuum concentration of the red seed solid matter is terminated after the solid content reaches 70-80%.

[0048] Compared with the prior art, the present invention also has the following technical effects:

[0049] In the prior art, flavonoid components with poor fat solubility are extracted from peony, rose, rose, etc., and flavonoid components have the function of inhibiting tyrosinase, and inhibiting the production of melanin by inhibiting tyrosinase activity, thereby achieving the treatment of excessive melanin deposition caused by certain skin diseases, and also having a whitening effect. However, the flavonoid components with poor fat solubility extracted from peony, rose, rose, etc. are difficult to be absorbed by the human cell wall. Therefore, in order to solve the above technical problems, the present invention obtains a hop extract having α-acid, extract (α-acid, β-acid) and polyphenols by high-temperature heating with hot steam and then freezing and sublimating. The α-acid, extract (α-acid, β-acid) and polyphenols can greatly improve the antioxidant capacity of the red seed composition;

[0050] At the same time, the organic acid components of the red seed extract are combined to bidirectionally synergistically inhibit tyrosinase activity and promote the renewal of epidermal keratinocytes. In order to further promote the organic acids in the red seed extract and the polyphenols of hops, the red seed composition is made to have antibacterial and antioxidant effects through the extract of the aerial stems and leaves of licorice. At the same time, it also contains more fat-soluble flavonoids, mainly isoflavones, dihydroflavonoids, and flavonol flavonoid aglycones. The flavonol flavonoid aglycone components have multiple pharmacological activities such as antioxidant, anti-inflammatory, and antibacterial. Moreover, aglycones are directly absorbed in the human blood, and their potency is 7 times that of glycosides.

[0051] Therefore, the present invention can not only give full play to the efficacy of each component, but also the components cooperate with each other to play a synergistic role, and play a role in four aspects: improving skin healthy circulation, removing free radicals in the body, improving pigmentation and moisturizing the skin, thereby achieving a more lasting and effective skin brightening effect. DETAILED DESCRIPTION

[0052] The following is further described in detail through specific implementation methods:

[0053] Now will be referred to in detail the embodiments disclosed in the present invention, although the present disclosure will be described in conjunction with the embodiments and / or examples, they are not intended to limit the present disclosure to these embodiments and / or examples. On the contrary, the present disclosure covers alternatives, modifications and equivalents.

[0054] Example 1

[0055] A red seed composition with whitening effect comprises: 20 parts by weight of hop extract, 25 parts by weight of red seed extract, 15 parts by weight of licorice aerial stem and leaf part extract, and 40 parts by weight of deionized water.

[0056] The extraction steps of hop extract include:

[0057] Step 1, subjecting the washed hops to hot steam treatment, and then quickly placing the hops in a freezer for freezing treatment to obtain solid hops, wherein the water content of the hops is 15%;

[0058] Step 2, the solid hops in step 2 are pounded into juice and ethanol solution is added to the juice, the juice and the ethanol solution are fully stirred at room temperature, and a mixed liquid is obtained after standing for 1.5 hours, the mixed liquid is subjected to vibration extraction and filtration, and ethanol is recovered from the filtrate to obtain hop liquid;

[0059] The juice and the ethanol solution are fully stirred and mixed, and then allowed to stand to obtain a mixed liquid, and the mixed liquid is subjected to ultrasonic-assisted ethanol solution extraction of hop liquid; the amount of ethanol solution used is 15 times the total mass of solid hops, and at the same time, in the process of using ultrasonic-assisted ethanol solution to extract the hop liquid, the extraction temperature is 50° C., the time is 15 minutes, and the ultrasonic frequency is 60KHz, so that the hops can be quickly extracted while maintaining the effective substance components in the hops;

[0060] Step 3: placing the hop liquid in step 3 in a freezer for freeze drying to obtain frozen hop powder, and sieving the frozen hop powder through a 110-mesh sieve to obtain a hop extract.

[0061] The extraction steps of red seed extract include:

[0062] Step 1: Place the cleaned red seeds in a freezer for freeze drying, so that the water content of the red seeds is 0.5%, and crush and sieve the freeze-dried red seeds to make 30-mesh red seed powder;

[0063] Step 2: preparing an extract with deionized water and ethanol solution in a ratio of 3:6, and soaking the red seed powder in the extract for 48 hours to obtain a red seed immersion solution;

[0064] Step 3, reflux extraction of the red seed immersion solution in step 2 for 30 minutes to obtain a red seed filtrate, and evaporate the red seed filtrate through a rotary evaporator to obtain a red seed extract;

[0065] The reflux extraction includes several cooling extraction sections and heating extraction sections, which are arranged alternately to form intermittent reflux extraction, wherein the extraction is stopped between the cooling extraction section and the heating extraction section, and then the temperature is raised for extraction once after cooling to room temperature, and the extraction temperature does not exceed 80°C. After extraction, the red seed filtrate is obtained by vacuum filtration.

[0066] The steps of extracting the aboveground stems and leaves of licorice include:

[0067] Step 1, washing the rhizomes and leaves on the aboveground stems and leaves of licorice, steam-heating the rhizomes and leaves after washing, and quickly placing the rhizomes and leaves in a freezer for freeze-drying after the steam-heating treatment, so that the water content in the rhizomes and leaves is 10%;

[0068] Step 2: Mix and crush the rhizomes and leaves obtained by freeze-drying in step 1 to form a mixture slurry, add ethanol solution to the mixture slurry, and extract the mixed extract by ultrasonic-assisted extraction;

[0069] Step 3: Filter the mixed extract to obtain a mixture precipitate, and place the mixture precipitate in a freezer for freeze-drying to obtain an extract of the aboveground stems and leaves of licorice.

[0070] Example 2

[0071] A red seed composition with whitening effect comprises: 15 parts by weight of hops extract, 20 parts by weight of red seed extract, 10 parts by weight of licorice aerial stem and leaf part extract, and 55 parts by weight of deionized water.

[0072] The extraction steps of hop extract include:

[0073] Step 1, subjecting the washed hops to hot steam treatment, and then quickly placing the hops in a freezer for freezing treatment to obtain solid hops, wherein the water content of the hops is 18%;

[0074] Step 2, pounding the solid hops in step 2 into juice and adding ethanol solution into the juice, stirring the juice and the ethanol solution at room temperature, standing for 2 hours to obtain a mixed liquid, extracting and filtering the mixed liquid by vibration, recovering ethanol from the filtrate, and obtaining hop liquid;

[0075] The juice and the ethanol solution are fully stirred and mixed, and then allowed to stand to obtain a mixed liquid, and the mixed liquid is subjected to ultrasonic-assisted ethanol solution extraction of hop liquid; the amount of ethanol solution used is 18 times the total mass of solid hops, and at the same time, in the process of using ultrasonic-assisted ethanol solution to extract the hop liquid, the extraction temperature is 55° C., the time is 25 minutes, and the ultrasonic frequency is 60KHz, so that the hops can be quickly extracted while maintaining the effective substance components in the hops;

[0076] Step 3: placing the hop liquid in step 3 in a freezer for freeze drying to obtain frozen hop powder, and sieving the frozen hop powder through a 120-mesh sieve to obtain a hop extract.

[0077] The extraction steps of red seed extract include:

[0078] Step 1: Place the cleaned red seeds in a freezer for freeze drying, so that the water content of the red seeds is 0.8%, and crush and sieve the freeze-dried red seeds to make 45-mesh red seed powder;

[0079] Step 2: preparing an extract with deionized water and ethanol solution in a ratio of 3:6, and soaking the red seed powder in the extract for 52 hours to obtain a red seed immersion solution;

[0080] Step 3, reflux extraction of the red seed immersion solution in step 2 for 30 minutes to obtain a red seed filtrate, and evaporate the red seed filtrate through a rotary evaporator to obtain a red seed extract;

[0081] The reflux extraction includes several cooling extraction sections and heating extraction sections, which are arranged alternately to form intermittent reflux extraction, wherein the extraction is stopped between the cooling extraction section and the heating extraction section, and then the temperature is raised for extraction once after cooling to room temperature, and the extraction temperature does not exceed 80°C. After extraction, the red seed filtrate is obtained by vacuum filtration.

[0082] The steps of extracting the aboveground stems and leaves of licorice include:

[0083] Step 1, washing the rhizomes and leaves on the aboveground stems and leaves of licorice, and then steam drying the rhizomes and leaves. After the steam drying treatment, the rhizomes and leaves are quickly placed in a freezer for freeze drying, so that the water content of the rhizomes and leaves is 8%;

[0084] Step 2: Mix and crush the rhizomes and leaves obtained by freeze-drying in step 1 to form a mixture slurry, add ethanol solution to the mixture slurry, and extract the mixed extract by ultrasonic-assisted extraction;

[0085] Step 3: Filter the mixed extract to obtain a mixture precipitate, and place the mixture precipitate in a freezer for freeze-drying to obtain an extract of the aboveground stems and leaves of licorice.

[0086] Example 3

[0087] A red seed composition with whitening effect comprises: 25 parts by weight of hop extract, 10 parts by weight of red seed extract, 5 parts by weight of licorice aerial stem and leaf part extract, and 60 parts by weight of deionized water.

[0088] The extraction steps of hop extract include:

[0089] Step 1, subjecting the washed hops to hot steam treatment, and then quickly placing the hops in a freezer for freezing treatment to obtain solid hops, wherein the water content of the hops is 20%;

[0090] Step 2, the solid hops in step 2 are pounded into juice and ethanol solution is added to the juice, the juice and the ethanol solution are fully stirred at room temperature, and a mixed liquid is obtained after standing for 2.5 hours, the mixed liquid is subjected to vibration extraction and filtration, and ethanol is recovered from the filtrate to obtain hop liquid;

[0091] The juice and the ethanol solution are fully stirred and mixed, and then allowed to stand to obtain a mixed liquid, and the mixed liquid is subjected to ultrasonic-assisted ethanol solution extraction of hop liquid; the amount of ethanol solution used is 20 times the total mass of solid hops, and at the same time, in the process of using ultrasonic-assisted ethanol solution to extract the hop liquid, the extraction temperature is 60° C., the time is 30 minutes, and the ultrasonic frequency is 60 KHz, so that the hops can be quickly extracted while maintaining the effective substance components in the hops;

[0092] Step 3: placing the hop liquid in step 3 in a freezer for freeze drying to obtain frozen hop powder, and sieving the frozen hop powder through a 130-mesh sieve to obtain a hop extract.

[0093] The extraction steps of red seed extract include:

[0094] Step 1: Place the cleaned red seeds in a freezer for freeze drying, so that the water content of the red seeds is 1%, and crush and sieve the freeze-dried red seeds to make 60-mesh red seed powder;

[0095] Step 2: preparing an extract with deionized water and ethanol solution in a ratio of 3:6, and soaking the red seed powder in the extract for 62 hours to obtain a red seed immersion solution;

[0096] Step 3, reflux extraction of the red seed immersion solution in step 2 for 30 minutes to obtain a red seed filtrate, and evaporate the red seed filtrate through a rotary evaporator to obtain a red seed extract;

[0097] The reflux extraction includes several cooling extraction sections and heating extraction sections, which are arranged alternately to form intermittent reflux extraction, wherein the extraction is stopped between the cooling extraction section and the heating extraction section, and then the temperature is raised for extraction once after cooling to room temperature, and the extraction temperature does not exceed 80°C. After extraction, the red seed filtrate is obtained by vacuum filtration.

[0098] The steps of extracting the aboveground stems and leaves of licorice include:

[0099] Step 1, washing the rhizomes and leaves on the aboveground stems and leaves of licorice, and then steam drying the rhizomes and leaves. After the steam drying treatment, the rhizomes and leaves are quickly placed in a freezer for freeze drying, so that the water content of the rhizomes and leaves is 5%;

[0100] Step 2: Mix and crush the rhizomes and leaves obtained by freeze-drying in step 1 to form a mixture slurry, add ethanol solution to the mixture slurry, and extract the mixed extract by ultrasonic-assisted extraction;

[0101] Step 3: Filter the mixed extract to obtain a mixture precipitate, and place the mixture precipitate in a freezer for freeze-drying to obtain an extract of the aboveground stems and leaves of licorice.

[0102] The red seed composition with whitening effect in Examples 1 to 3 is applied to inhibit tyrosinase, including preparing skin care cosmetics or medicines by the preparation method of the composition, and the preparation method comprises the following steps:

[0103] Heat deionized water to 50° C., 60° C. or 70° C., and during the heating of the deionized water, add the red seed composition in step 1 into the deionized water, stir for 20 minutes, and then stand for separation and passivation to obtain a red seed composition mixed solution, and vacuum concentrate the red seed composition mixed solution to obtain a red seed solid;

[0104] Take 20 or 30 parts of deionized water, add 0.5%, 0.8% or 1% of fenugreek oil into the deionized water to obtain an oil phase raw material, slowly add the red seed solid matter in step 2 into the oil phase raw material, so that the red seed solid matter is fully emulsified in the oil phase raw material, continue to slowly stir, and cool to 35° C., 40° C. or 45° C.

[0105] Comparative Example 1

[0106] Compared with Example 1, the difference is that this comparative example provides a composition: 100 parts of hop extract.

[0107] Comparative Example 2

[0108] Compared with Example 1, the difference is that this comparative example provides a composition: 100 portions of red seed extract.

[0109] Comparative Example 3

[0110] Compared with Example 1, the difference is that this comparative example provides a composition: 100 parts of extracts from the aboveground stems and leaves of licorice.

[0111] Comparative Example 4

[0112] Compared with Example 1, the difference is that this comparative example provides a composition: 20 parts by weight of hop extract and 25 parts by weight of red seed extract. The extraction steps of hop extract and red seed extract are the same as those of Example 1.

[0113] Comparative Example 5

[0114] Compared with Example 1, the difference is that this comparative example provides a composition: 20 parts by weight of hop extract and 15 parts by weight of licorice aerial stem and leaf extract. The extraction steps of hop extract and licorice aerial stem and leaf extract are the same as those in Example 1.

[0115] Comparative Example 6

[0116] Compared with Example 1, the difference is that this comparative example provides a composition: the extract of the aboveground stems and leaves of licorice is 15 parts by weight and the red seed extract is 25 parts by weight. The extraction steps of the extract of the aboveground stems and leaves of licorice and the red seed extract are the same as those in Example 1.

[0117] Comparative Example 7

[0118] Compared with Example 1, the difference is that this comparative example provides a composition: 20 parts by weight of peony flowers, 25 parts by weight of rose flowers, 15 parts by weight of rose flowers, and 40 parts by weight of deionized water.

[0119] Effect verification:

[0120] The compositions prepared in Examples 1-3 and Comparative Examples 1-7 are numbered 1-10.

[0121] (1) Tyrosinase activity inhibition test:

[0122] The tyrosinase inhibition rate (I) was tested with L-Tyr and L-Dopa as substrates. In the process of tyrosine conversion to melanin, the catalytic action of tyrosinase mainly occurred in the process of tyrosine conversion to L-DOPA and L-DOPA conversion to dopaquinone. Dopaquinone is a colored substance that absorbs at 475nm and its content can be measured using a spectrophotometer.

[0123] The compositions numbered 1-10 were used to prepare test solutions with a concentration of 3 mg / mL respectively; L-Tyr and L-Dopa were used as substrates respectively, and the compositions numbered 1-10 were used to prepare test solution groups according to Table 1, and the absorbance value was detected by a spectrophotometer, and the absorbance was detected at a wavelength of 475 nm. According to the detected absorbance value, the inhibition rate (I) of the compositions numbered 1-10 on tyrosinase activity was calculated according to formula (1). The test results are shown in Table 2.

[0124] Table 2 Composition of samples to be tested (mL)

[0125]

[0126] The calculation formula of tyrosinase inhibition rate (I) is as follows:

[0127] I=(1-(Ac-Ad) / (Aa-Ab))x100%

[0128] Wherein, Aa: is the absorbance value of the mixed solution of group a; Ab: is the absorbance value of the mixed solution of group b; Ac: is the absorbance value of the mixed solution of group c; Ad: is the absorbance value of the mixed solution of group d.

[0129] Table 2 Tyrosinase activity inhibition rate (I)

[0130]

[0131] It can be seen from Table 2 that whether L-Tyr or L-Dopa is used as a substrate, the tyrosinase activity inhibition rate of the composition for inhibiting tyrosinase activity prepared in Examples 1-3 is significantly higher than that of the composition for inhibiting tyrosinase activity prepared in Comparative Examples 1-7, which proves that the tyrosinase activity inhibition ability of the composition for inhibiting tyrosinase activity prepared in Examples 1-3 is significantly better than that of the composition for inhibiting tyrosinase activity prepared in Comparative Examples 1-7.

[0132] (2) Determination of the inhibitory effect of the composition on melanin in melanocytes

[0133] 1. Select melanoma tumor B16 cells in the logarithmic growth phase and adjust the cell suspension concentration to 2×10 4The cells were inoculated at a concentration of 10 cells / mL in a 96-well culture plate, wherein the volume of each well in the 96-well culture plate was 100 μL, and the wells without cells were filled with sterile PBS;

[0134] 2. Using DMSO as solvent, prepare the compositions numbered 1-10 into standard test solutions with a concentration of 150 μg / mL;

[0135] 3. During the incubation process, the drug culture medium (96-well plate) was changed once in the middle. After 72 hours, the supernatant was discarded, and the cells were washed twice with PBS. The cells were digested with 0.25% trypsin, harvested, and placed in a centrifuge tube. The harvested cells were centrifuged at 1000 r / min, the supernatant was discarded, 1 mL PBS solution was added and centrifuged and washed once, and the supernatant was discarded.

[0136] 4. Add 100 μL of PBS solution, shake, mix well, add 200 μL of NaOH (1 mol / L) containing 10% DMSO preheated at 80°C, heat at 80°C for 3 h, shake once every half an hour, transfer 100 μL to a new 96-well plate, and detect the light absorbance at a wavelength of 405 nm.

[0137] According to the detected absorbance, the melanin synthesis inhibition rate formula was used to calculate the melanin production inhibition rate of the composition in melanocytes. The results are shown in Table 3.

[0138] Melanin synthesis inhibition rate calculation formula:

[0139] Melanin synthesis inhibition rate = (1-(drug well absorbance value ÷ drug well cell density)) × 100%

[0140] The absorbance value of the drug well is the absorbance added with the standard test solution; the cell density of the drug well is the concentration of B16 cells added with the standard test solution.

[0141] Table 3 Inhibition rate of melanin formation in melanocytes by the composition

[0142]

[0143] It can be seen from Table 3 that at a concentration of 250 μg / mL, the inhibition rate of melanin production in melanocytes by the composition for inhibiting tyrosinase activity prepared in Examples 1-3 was significantly higher than that by the composition for inhibiting tyrosinase activity prepared in Comparative Examples 1-7; this proves that the ability of the composition for inhibiting tyrosinase activity prepared in Examples 1-3 to inhibit melanin production in melanocytes is significantly better than that by the composition for inhibiting tyrosinase activity prepared in Comparative Examples 1-7.

[0144] (3) Oxidative stability test of oils and fats

[0145] By measuring the length of the induced oxidation time of the oil sample, the oxidative stability of the oil can be judged. The longer the induction time, the better the oxidative stability of the oil, and vice versa, the oxidative stability of the oil is poor.

[0146] The test was conducted using a 743-type Rancimat oil oxidation tester under the following test conditions: temperature 110°C, air flow 10L / h. 3g of each composition numbered 1-10 was weighed into a reaction tube, and 50mL of distilled water was added to the receiving tank. When the temperature of the heating tank reached the test temperature, the conductivity curve of the sample in the reaction tube was measured. The maximum second-order derivative of the conductivity was taken as the test end point, thereby evaluating the oil stability period of the sample. The results are shown in Table 4.

[0147] 1 2 3 4 5 6 7 8 9 10 110℃ induction time (h) 60.9 80.05 75.4 20.8 24.5 28.2 31.5 40 35.5 10

[0148] From the results in Table 4, it can be seen that at 110° C., the oxidation induction time of the compositions prepared in Examples 1-3 is between 60.9 and 80.5 h, while the oxidation induction time of the compositions prepared in Comparative Examples 1-7 is between 10 and 40 h. Therefore, compared with the compositions in Comparative Examples 1-7, the oxidation stability of the compositions of the present invention is significantly improved.

[0149] The above are only embodiments of the present invention, and the common knowledge such as the known specific technical solutions and / or characteristics in the solution are not described in detail here. It should be pointed out that for those skilled in the art, several modifications and improvements can be made without departing from the technical solution of the present invention, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. A red seed composition with whitening effect, characterized in that: The invention comprises hop extract, red seed extract and licorice aerial part extract, wherein the hop extract accounts for 15-25 parts by weight, the red seed extract accounts for 10-25 parts by weight, the licorice aerial stem and leaf part extract accounts for 5-15 parts by weight, and deionized water accounts for 40-60 parts by weight.

2. A red seed composition with whitening effect according to claim 1, characterized in that: The hop extract is 20 parts by weight, the red seed extract is 25 parts by weight, the licorice aerial stem and leaf extract is 15 parts by weight, and the deionized water is 40 parts by weight.

3. A red seed composition with whitening effect according to claim 1, characterized in that: The hop extract is 15 parts by weight, the red seed extract is 20 parts by weight, the licorice aerial stem and leaf extract is 10 parts by weight, and the deionized water is 55 parts by weight.

4. A red seed composition with whitening effect according to claim 1, characterized in that: The hop extract is 25 parts by weight, the red seed extract is 10 parts by weight, the licorice aerial stem and leaf extract is 5 parts by weight, and the deionized water is 60 parts by weight.

5. A red seed composition with whitening effect according to any one of claims 1 to 4, characterized in that: The extraction steps of hop extract include: Step 1, subjecting the cleaned hops to hot steam treatment, and then quickly placing the hops in a freezer for freezing treatment to obtain solid hops, wherein the water content of the hops is 15%-20%; Step 2, the solid hops in step 2 are pounded into juice and ethanol solution is added to the juice, the juice and the ethanol solution are fully stirred at room temperature, and a mixed liquid is obtained after standing for 1-3 hours, the mixed liquid is subjected to vibration extraction and filtration, and ethanol is recovered from the filtrate to obtain hop liquid; Step 3: placing the hop liquid in step 3 in a freezer for freeze drying to obtain frozen hop powder, and sieving the frozen hop powder with 100-130 mesh to obtain hop extract.

6. A red seed composition with whitening effect according to claim 5, characterized in that: In step 2, the juice and the ethanol solution are fully stirred and mixed and then allowed to stand to obtain a mixed liquid, and the mixed liquid is subjected to ultrasonic-assisted extraction of the hop liquid with the ethanol solution.

7. A red seed composition with whitening effect according to any one of claims 1 to 4, characterized in that: The extraction steps of red seed extract include: Step 1: Place the cleaned red seeds in a freezer for freeze drying, so that the water content of the red seeds is 0.5-1%, and crush and sieve the freeze-dried red seeds to make 30-60 mesh red seed powder; Step 2: preparing an extract with deionized water and ethanol solution in a ratio of 3:6, and soaking the red seed powder in the extract for 48-62 hours to obtain a red seed immersion solution; Step 3: reflux the red seed immersion solution in step 2 for 30 minutes to obtain a red seed filtrate, and evaporate the liquid of the red seed filtrate through a rotary evaporator to obtain a red seed extract.

8. A red seed composition with whitening effect according to claim 7, characterized in that: In step three, the reflux extraction includes several cooling extraction sections and heating extraction sections, which are arranged alternately to form intermittent reflux extraction, wherein the extraction is stopped between the cooling extraction sections and the heating extraction sections, and then the temperature is increased for extraction once after cooling to room temperature, and the extraction temperature does not exceed 80°C. After extraction, the red seed filtrate is obtained by vacuum filtration.

9. A red seed composition with whitening effect according to any one of claims 1 to 4, characterized in that: The steps of extracting the aboveground stems and leaves of licorice include: Step 1, washing the rhizomes and leaves on the aboveground stems and leaves of licorice, and then steam drying the rhizomes and leaves. After the steam drying treatment, the rhizomes and leaves are quickly placed in a freezer for freeze drying, so that the water content of the rhizomes and leaves is 5-10%; Step 2: Mix and crush the rhizomes and leaves obtained by freeze-drying in step 1 to form a mixture slurry, add ethanol solution to the mixture slurry, and extract the mixed extract by ultrasonic-assisted extraction; Step 3: Filter the mixed extract to obtain a mixture precipitate, and place the mixture precipitate in a freezer for freeze-drying to obtain an extract of the aboveground stems and leaves of licorice.

10. A red seed composition with whitening effect for inhibiting tyrosinase, characterized in that: include: The red seed composition with whitening effect as claimed in any one of claims 1 to 9 is used to prepare skin care cosmetics or medicines by the preparation method of the composition, and the preparation method comprises the following steps: Step 1: Red seed composition: based on weight components, take 15-25 parts of hops, 10-25 parts of red seed extract, and 5-15 parts of licorice aerial stem and leaf extract; Step 2: Taking 40-60 parts of deionized water by weight, heating the deionized water to 50-70° C., during the heating of the deionized water, adding the red seed composition in step 1 into the deionized water, stirring for 20 minutes, and then standing for separation and passivation to obtain a red seed composition mixed solution, and vacuum concentrating the red seed composition mixed solution to obtain a red seed solid; Step 3: Take 20-30 parts of deionized water by weight, and add 0.5-1% of fenugreek oil to the deionized water to obtain an oil phase raw material, slowly add the red seed solid matter in step 2 to the oil phase raw material, so that the red seed solid matter is fully emulsified in the oil phase raw material, continue to slowly stir, and cool to 35-45° C.

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