Plant sunscreen synergistic composition suitable for sensitive skin and application

Abstract

The invention discloses a plant sunscreen synergistic composition and application thereof, and belongs to the technical field of cosmetics. The plant sunscreen synergistic composition provided by the invention is prepared from the following components in parts by mass: 0.1 to 1 part of rhizoma cimicifugae extract, 0.3 to 1 part of folium cortex eucommiae extract, 1 to 3 parts of rambutan peel extract and 5 to 10 parts of rhizoma cyperi root extract. According to the invention, the components in a proper mass ratio are selected for compounding, so that the obtained composition has a good ultraviolet absorption effect and also has excellent anti-inflammatory and soothing effects. Moreover, when the sunscreen cream is subsequently added into a sunscreen product, a physical sunscreen agent and a chemical sunscreen agent in the sunscreen cream can be effectively stabilized, the sunscreen effects of the physical sunscreen agent and the chemical sunscreen agent can be enhanced, the durability of the sunscreen product can be improved, and the repair of the sunscreen product to the after-sun skin can be enhanced.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and in particular relates to a plant sunscreen synergistic composition and application thereof. Background Art

[0002] Sunscreen products are designed to reduce skin damage from UV rays by adding sunscreens that reflect or absorb UV radiation. Sunscreen products need to add a large amount of physical and chemical sunscreens to achieve adequate sun protection, but too much sunscreen can cause varying degrees of damage to the skin and is not suitable for people with sensitive skin.

[0003] At the same time, the existing sunscreen function is relatively simple. Without sufficient sunscreen, the skin will be prone to photoburn and photosensitivity, leading to symptoms such as redness and inflammation of the skin. Further use of sunscreen products that add a large amount of physical sunscreens and chemical sunscreens will further aggravate skin inflammation, allergies and other reactions. Summary of the invention

[0004] The purpose of the present invention is to overcome the deficiencies of the above-mentioned prior art and provide a plant sunscreen synergistic composition and application which has excellent ultraviolet absorption effect and anti-inflammatory soothing effect, can effectively enhance the sunscreen ability and stability of physical sunscreens and chemical sunscreens, and can repair sensitive skin after sun exposure.

[0005] To achieve the above object, in a first aspect of the present invention, the present invention provides a plant sunscreen synergistic composition, the plant sunscreen synergistic composition comprising the following components in parts by weight:

[0006] 0.1-1 parts of Cimicifuga extract, 0.3-1 parts of Eucommia leaf extract, 1-3 parts of Rambutan peel extract, and 5-10 parts of Cyperus rotundus root extract.

[0007] The present invention selects components in a suitable mass ratio, specifically Cimicifuga heracleifolia extract, Eucommia ulmoides leaf extract, Rambutan leaf peel extract and Cyperus rotundus root extract. The four ingredients have excellent compounding effect, and on the basis of achieving good ultraviolet absorption ability under synergistic effect, also have excellent anti-inflammatory effect; and, when subsequently added into sunscreen products, the sunscreen effects of physical sunscreen agents and chemical sunscreen agents in the original sunscreen products can be effectively enhanced, the durability of the sunscreen products can be improved, and the sunscreen products can be enhanced to repair the skin after sun exposure.

[0008] Specifically, the present invention has found that the cycloaltinane-type triterpene compounds contained in the Cimicifuga extract are its characteristic active ingredients, which have antibacterial and anti-inflammatory effects. In addition, the cimicifuga in the Cimicifuga can regulate the expression of some genes to inhibit the pathological changes of inflammatory cells, showing anti-inflammatory and anti-allergic effects. The cinnamic acid derivatives therein can also protect the basement membrane from inflammatory damage by inhibiting the activity of neutrophil elastase. For sun protection, the active substances in the Cimicifuga extract, such as ferulic acid itself, have a certain absorption capacity for ultraviolet rays, and can also have a good synergistic ability for physical sunscreens, especially titanium dioxide and zinc oxide. In addition to having excellent antioxidant and anti-inflammatory abilities, the flavonoids and phenolic acid components in Eucommia leaf extract also have certain ultraviolet absorption capabilities. In addition, Eucommia leaf extract is also rich in chlorogenic acid, which not only has good ultraviolet absorption capabilities, but also has a good synergistic effect on chemical sunscreens, especially diethylaminohydroxybenzoyl hexyl benzoate; at the same time, eucommia leaf extracts such as aucubin, eucommia alcohol, geniposide and other iridoid terpenes also have anti-inflammatory and analgesic effects. Rambutan peel extract contains ellagic acid, quercetin and other components. On the basis of having certain ultraviolet absorption capabilities, the above components can also minimize the photolysis of chemical sunscreens, especially ethylhexyl methoxycinnamate, to avoid the loss of photolysis content and cause the sunscreen durability to decrease, and enhance its long-lasting sunscreen ability; in addition, the polyphenols contained in rambutan peel can increase the synthesis of phospholipids and ceramides in the epidermis, strengthen the barrier function of the stratum corneum to prevent epidermal dehydration, and achieve good repair and soothing effects. The extract of Cyperus rotundus root, on the basis of having a certain ability to absorb ultraviolet rays, can also reduce inflammation of epidermal keratinocytes caused by ultraviolet rays and promote the metabolism of epidermal keratinocytes, and has an excellent soothing and repairing effect. In addition, the present invention has found through a large number of creative experiments that the above four components are compounded in a specific mass ratio, and have a good synergistic effect with each other, and the obtained product has better ultraviolet absorption ability and anti-inflammatory effect.

[0009] As a preferred embodiment of the plant sunscreen synergistic composition of the present invention, the plant sunscreen synergistic composition comprises the following components in parts by weight: 0.5-0.6 parts of Cimicifuga extract, 0.4-0.5 parts of Eucommia ulmoides leaf extract, 1.5-2 parts of rambutan peel extract, and 7-8 parts of Cyperus rotundus root extract.

[0010] The present invention has been found that the mass parts of the components in the plant sunscreen synergistic composition will affect the comprehensive performance of the composition. When the mass parts of the components in the plant sunscreen synergistic composition are further selected to be within the above-mentioned range, the obtained composition has better ultraviolet absorption capacity and better anti-inflammatory and soothing effects. When applied to sunscreen products, the sunscreen ability of the sunscreen products is more significantly improved, the sunscreen durability is stronger, and the after-sun repair effect is also better.

[0011] As a preferred embodiment of the plant sunscreen synergistic composition of the present invention, in the plant sunscreen synergistic composition, the mass percentage of the rambutan peel extract is 11-22%.

[0012] Illustratively, in the plant sunscreen synergistic composition, the mass percentage of rambutan peel extract can be any point value or any two point range values ​​between 11-17%, for example, it can be 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, etc.

[0013] The present invention has found that in the plant sunscreen synergistic composition, the mass percentage of rambutan peel extract will not only affect the ultraviolet absorption ability of the composition, but also affect the anti-inflammatory and soothing effects of the composition. When the mass percentage of rambutan peel extract is further selected to be within the above range, the comprehensive effect of the obtained plant sunscreen synergistic composition is better, and when used in the preparation of sunscreen products, the obtained sunscreen product has a stronger ability to repair sun-exposed skin, and has better sunscreen ability and sunscreen durability.

[0014] As a preferred embodiment of the plant sunscreen synergistic composition of the present invention, the mass percentage of chlorogenic acid in the eucommia leaf extract is ≥5%.

[0015] It should be noted that the mass percentage of chlorogenic acid in the Eucommia ulmoides leaf extract is obtained by the high performance liquid chromatography test described in the content determination of Eucommia ulmoides leaf in the 2020 edition of the Chinese Pharmacopoeia. The test method is as follows:

[0016] Chromatographic conditions: octadecylsilane bonded silica gel is used as filler; acetonitrile-0.4% phosphoric acid solution (13:87) is used as mobile phase; detection wavelength is 327nm. Preparation of reference substance: Take an appropriate amount of chlorogenic acid reference substance, accurately weigh it, place it in a brown volumetric flask, add 50% methanol to make a solution containing 50μg per 1ml, and obtain it. Preparation of test sample: Take about 1g of the test sample powder, accurately weigh it, place it in a stoppered conical flask, accurately add 25ml of 50% methanol, weigh the weight, heat and reflux for 30 minutes, cool, weigh the weight again, make up the lost weight with 50% methanol, shake well, filter, and take the filtrate. Determination: Accurately aspirate 10μl of the reference solution and the test solution respectively, inject them into the liquid chromatograph, and determine them.

[0017] Exemplarily, the mass percentage of chlorogenic acid in the Eucommia leaf extract can be any point value ≥5% or any two-point range value, for example, it can be 5-10%, 5-15%, 5-20%, 2-25%, etc., or it can be 5%, 7%, 9%, 10%, 12%, 14%, 16%, 18%, 20%, 22%, 25%, etc.

[0018] The present invention has found that when the mass percentage of chlorogenic acid in the Eucommia leaf extract is further selected to be within the above range, it can not only further enhance the ultraviolet absorption capacity of the composition, but also, when subsequently added to the preparation of sunscreen products, can better enhance the synergy of the chemical sunscreen agents in the sunscreen products, thereby effectively improving the sunscreen ability and sunscreen durability of the sunscreen products.

[0019] As a preferred embodiment of the plant sunscreen synergistic composition of the present invention, the preparation method of the Cimicifuga extract comprises the following steps: adding Cimicifuga to an ethanol aqueous solution for microwave extraction, filtering after the extraction, collecting the filtrate and concentrating and drying it to obtain the Cimicifuga extract.

[0020] As a preferred embodiment of the plant sunscreen synergistic composition of the present invention, in the preparation method of the Cimicifuga extract, the mass percentage of ethanol in the ethanol aqueous solution is 60-80%, and the mass volume ratio of Cimicifuga to the ethanol aqueous solution is 1g: (15-25)mL.

[0021] As a preferred embodiment of the plant sunscreen synergistic composition of the present invention, in the preparation method of the Cimicifuga extract, the power of microwave extraction is 380-390W and the time is 80-100s.

[0022] Preferably, the process further comprises the steps of drying, crushing and sieving before adding the Cimicifuga to the ethanol aqueous solution.

[0023] The present invention has found that the preparation method of the Cimicifuga extract will affect the content and type of active ingredients in the Cimicifuga extract, and further affect the interaction between the Cimicifuga extract and other components. When the preparation method of the Cimicifuga extract is further selected as the method described in the present invention, the obtained composite effect of the Cimicifuga extract and other components is better, the obtained plant sunscreen synergistic composition has a better comprehensive effect, and when subsequently applied to sunscreen products, the obtained sunscreen product also has a better effect.

[0024] As a preferred embodiment of the plant sunscreen synergistic composition of the present invention, the preparation method of the Cyperus rotundus root extract comprises the following steps: taking the Cyperus rotundus root, crushing it and adding it to an ethanol aqueous solution, heating and extracting it, filtering and concentrating it after the extraction is completed to obtain the Cyperus rotundus root extract.

[0025] As a preferred embodiment of the plant sunscreen synergistic composition of the present invention, in the preparation method of the Cyperus rotundus root extract, the mass percentage of ethanol in the ethanol aqueous solution is 45-55%, and the mass volume ratio of Cyperus rotundus root and ethanol aqueous solution is 1g: (3-7)mL.

[0026] As a preferred embodiment of the plant sunscreen synergistic composition of the present invention, in the preparation method of the Cyperus rotundus root extract, the heating extraction temperature is 65-75° C. and the time is 1-2 h.

[0027] Preferably, the method further comprises the steps of drying, crushing and sieving before adding the Cyperus rotundus roots to the ethanol aqueous solution.

[0028] The present invention has found that the preparation method of the Cyperus rotundus root extract will affect the content and type of active ingredients in the Cyperus rotundus root extract, and further affect the interaction between the Cyperus rotundus root extract and other components. When the preparation method of the Cyperus rotundus root extract is further selected as the method described in the present invention, the obtained compounding effect of the Cyperus rotundus root extract and other components is better, the obtained plant sunscreen synergistic composition has better soothing effect, and when subsequently used in the preparation of sunscreen products, the obtained sunscreen products have better sunscreen effect and after-sun repair effect.

[0029] In the second aspect of the present invention, the present invention provides an application of the plant sunscreen synergistic composition in the preparation of sunscreen products.

[0030] The plant sunscreen synergistic composition provided by the present invention has good ultraviolet absorption ability and anti-inflammatory effect. When preparing sunscreen products, it can also effectively increase the sunscreen ability of physical sunscreens and chemical sunscreens in the sunscreen products and improve the stability of chemical sunscreens. Therefore, it can be widely used in the preparation of sunscreen products. The obtained sunscreen products not only have good and lasting sunscreen ability, but also have excellent after-sun repair effect.

[0031] In the third aspect of the present invention, the present invention provides a sunscreen lotion, which includes the following components in mass percentage: 10-15% of the plant sunscreen synergistic composition of the present invention, 5-7% physical sunscreen, 10-15% chemical sunscreen, 10-15% emollient, 2-3% film former, 5-12% humectant, 0.1-0.5% thickener, 0.5-2% preservative, 1-5% emulsifier, and the balance water.

[0032] The present invention has been found through research that by adding the plant sunscreen synergistic composition of the present invention to the sunscreen lotion provided by the present invention, the plant sunscreen synergistic composition and the physical sunscreen and the chemical sunscreen have good synergistic effects, which can not only effectively improve the sunscreen ability of the sunscreen lotion and enhance the long-lasting sunscreen ability of the sunscreen lotion, but also effectively repair the skin after sun exposure, achieving an excellent effect of soothing the skin after sun exposure.

[0033] As a preferred embodiment of the sunscreen lotion of the present invention, the physical sunscreen includes zinc oxide and titanium dioxide; the chemical sunscreen includes diethylaminohydroxybenzoyl hexyl benzoate, ethylhexyl methoxycinnamate, bis-ethylhexyloxyphenol methoxyphenyl triazine and ethylhexyl triazone.

[0034] The present invention has been found in research that when physical sunscreens and chemical sunscreens are further selected as the above-mentioned types, the plant sunscreen synergistic composition has a better synergistic effect on the above-mentioned types of physical sunscreens, as well as a better synergistic and stabilizing effect on the above-mentioned types of chemical sunscreens. Therefore, not only the sunscreen ability and sunscreen durability of the product are more significantly improved, but the repair effect of the resulting sunscreen product is also better.

[0035] As a preferred embodiment of the sunscreen lotion of the present invention, the emollient includes at least one of dicaprylyl carbonate and diisopropyl sebacate.

[0036] As a preferred embodiment of the sunscreen lotion of the present invention, the film-forming agent includes at least one of trimethylsiloxysilicate and cyclopentasiloxane.

[0037] As a preferred embodiment of the sunscreen lotion of the present invention, the moisturizer includes at least one of glycerin, 1,3-butylene glycol, and 1,2-pentanediol.

[0038] As a preferred embodiment of the sunscreen lotion of the present invention, the thickener includes at least one of xanthan gum, carbomer, and sodium polyacrylate.

[0039] As a preferred embodiment of the sunscreen lotion of the present invention, the preservative includes at least one of p-hydroxyacetophenone and hexylene glycol.

[0040] As a preferred embodiment of the sunscreen lotion of the present invention, the emulsifier includes at least one of polyglyceryl-10 oleate, polysorbate 60, and polyglyceryl-3 diisostearate.

[0041] Compared with the prior art, the present invention has the following beneficial effects:

[0042] The present invention selects components of appropriate mass ratio, specifically Cimicifuga extract, Eucommia leaf extract, Rambutan leaf peel extract, and Cyperus rotundus root extract. The four ingredients have excellent compounding effect, and the obtained composition has good ultraviolet absorption effect and excellent anti-inflammatory and soothing effect. Moreover, when it is subsequently added to sunscreen products, it can effectively stabilize the physical sunscreen and chemical sunscreen in the sunscreen lotion, and can also enhance the sunscreen effect of the physical sunscreen and the chemical sunscreen, improve the durability of the sunscreen product, and can enhance the repair of the sunscreen product to the skin after sun exposure. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] Figure 1 This is a before and after comparison of volunteers using the sunscreen lotion in Application Example 1 in Effect Example 2;

[0044] Figure 2 This is a before and after comparison of volunteers using the sunscreen lotion in Comparative Application Example 1 in Effect Example 2;

[0045] Figure 3 This is a before and after comparison of volunteers using the sunscreen in the blank example in Effect Example 2. DETAILED DESCRIPTION

[0046] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0047] Unless otherwise specified, the reagents, methods and equipment used in the present invention are all conventional reagents, methods and equipment in the art; and unless otherwise specified, the raw materials used in parallel experiments are from the same batch of raw materials.

[0048] Cimicifuga extract 1: Homemade, the preparation method includes the following steps:

[0049] Commercially available Cimicifuga heracleifolia was dried, crushed and passed through an 80-mesh sieve, the sieve residue was collected, added to a 70% by mass ethanol aqueous solution (the mass volume ratio of the sieve residue to the ethanol aqueous solution was 1 g: 20 mL), and microwaved for 90 seconds at 385 W. After microwave extraction, the filtrate was filtered, and the filtrate was collected and concentrated at 45° C., and then dried in a vacuum drying oven at 60° C. to obtain Cimicifuga heracleifolia extract 1;

[0050] Cimicifuga extract 2: Homemade, the preparation method includes the following steps:

[0051] Commercially available Cimicifuga heracleifolia was dried, crushed and passed through an 80-mesh sieve, the sieve residue was collected and added to a 30% by mass ethanol aqueous solution (the mass volume ratio of the sieve residue to the ethanol aqueous solution was 1 g: 20 mL), and microwaved for 110 s at 400 W. After microwave extraction, the mixture was filtered, the filtrate was collected and concentrated at 45° C., and then dried in a vacuum drying oven at 60° C. to obtain Cimicifuga heracleifolia extract 2;

[0052] Cimicifuga extract 3: Homemade, the preparation method includes the following steps:

[0053] Commercially available Cimicifuga heracleifolia was dried, crushed and passed through an 80-mesh sieve, the sieve residue was collected and added to a 70% ethanol aqueous solution (the mass volume ratio of the sieve residue to the ethanol aqueous solution was 1 g: 20 mL), and ultrasonically extracted for 30 min at 80 W and 30 ° C. After the ultrasonic extraction, the filtrate was filtered, and the filtrate was collected and concentrated at 45 ° C, and then dried in a vacuum drying oven at 60 ° C to obtain Cimicifuga heracleifolia extract 3;

[0054] Eucommia leaf extract 1: purchased from Changsha Shanghe Biotechnology Co., Ltd., with a mass percentage of chlorogenic acid of 5%;

[0055] Eucommia leaf extract 2: Homemade, take commercially available Eucommia leaves, dry them, crush them through a 60-mesh sieve, collect the sieve material, add them into water (the mass volume ratio of the sieve material and water is 1 g:15 mL), stir at 90°C for 30 min, filter after extraction, collect the filtrate and concentrate it at 60°C, then dry it in a vacuum drying oven at 60°C to obtain Eucommia leaf extract 2, and the mass percentage of chlorogenic acid was measured to be 2%.

[0056] Rambutan peel extract: purchased from BASF under the trade name Nephydrat TM BC10061;

[0057] Cyperus rotundus root extract 1: Homemade, the preparation method comprises the following steps:

[0058] The commercially available Cyperus rotundus root was dried, crushed and passed through an 80-mesh sieve, the sieve residue was collected and added to a 50% ethanol aqueous solution (the mass volume ratio of the sieve residue to the ethanol aqueous solution was 1 g: 5 mL), stirred at 70°C for 1.5 h, filtered after the heating extraction, and the filtrate was collected and concentrated at 45°C to obtain Cyperus rotundus root extract 1;

[0059] Cyperus rotundus root extract 2: Homemade, the preparation method comprises the following steps:

[0060] Take the commercially available Cyperus rotundus root, dry it, crush it and pass it through an 80-mesh sieve. Collect the sieve material and add it to an 80% ethanol aqueous solution (the mass volume ratio of the sieve material to the ethanol aqueous solution is 1g:5mL), stir it at 40°C for 1.5h, filter it after the heating extraction is completed, collect the filtrate and concentrate it at 45°C to obtain Cyperus rotundus root extract 2.

[0061] Examples 1-8 and Comparative Examples 1-6

[0062] The embodiments and comparative examples of the present invention provide a plant sunscreen synergistic composition, and the components (parts by weight) of the composition are shown in Table 1;

[0063] Table 1

[0064]

[0065]

[0066] The preparation method of the plant sunscreen enhancement composition provided in Example 1 is: mixing the components evenly to obtain the plant sunscreen enhancement composition.

[0067] The preparation methods of the plant sunscreen enhancement compositions provided in Examples 2-8 and Comparative Examples 1-6 are consistent with that in Example 1.

[0068] Comparative Example 7

[0069] The comparative example of the present invention provides a plant sunscreen enhancement composition. The only difference between the plant antiperspirant sunscreen repair composition and Example 1 is that the Cimicifuga racemosa root extract is used to replace the Cimicifuga extract 1.

[0070] Comparative Example 8

[0071] The comparative example of the present invention provides a plant sunscreen enhancement composition, and the only difference between the plant antiperspirant sunscreen repair composition and Example 1 is that the Eucommia bark extract is used to replace the Eucommia leaf extract 1.

[0072] Comparative Example 9

[0073] The comparative example of the present invention provides a plant sunscreen enhancement composition, and the only difference between the plant antiperspirant sunscreen repair composition and Example 1 is that the rambutan peel extract is replaced by the litchi peel extract.

[0074] Comparative Example 10

[0075] The comparative example of the present invention provides a plant sunscreen enhancement composition, wherein the plant antiperspirant sunscreen repair composition is different from Example 1 only in that the Cyperus rotundus root extract 1 is replaced by the Cyperus rotundus root extract.

[0076] Application Example 1-12, Comparative Application Example 1-10

[0077] The application examples of the present invention and the comparative application examples provide a sunscreen lotion, the components (mass percentage) of the sunscreen lotion are shown in Table 2; wherein the plant sunscreen synergistic compositions used in application examples 1-8 are respectively the plant sunscreen synergistic compositions prepared in embodiments 1-8, and the plant sunscreen synergistic compositions used in comparative application examples 1-10 are respectively the plant sunscreen synergistic compositions prepared in comparative examples 1-10; the plant sunscreen synergistic compositions used in application examples 9-12 are the plant sunscreen synergistic compositions prepared in embodiment 1;

[0078] In addition, a blank application example is set, and the only difference between the blank application example and application example 1 is that the plant sunscreen synergistic composition is not added;

[0079] Table 2

[0080]

[0081] The method for preparing the sunscreen lotion provided in Application Example 1 comprises the following steps:

[0082] The chemical sunscreen and the emulsifier are mixed and heated to 85°C for homogenization, and then cooled to 45°C, and then a film former, an emollient and a physical sunscreen are added to form a uniform oil phase, and then a moisturizer, a thickener and water are added at 25°C for emulsification, and after the emulsification is completed, a plant sunscreen synergistic composition and a preservative are added and stirred to obtain a sunscreen lotion;

[0083] The preparation methods of the sunscreen lotions provided in Application Examples 2-12, Comparative Application Examples 1-10 and Blank Application Examples are consistent with that in Application Example 1.

[0084] Effect Example 1

[0085] The performance of the plant sunscreen synergistic composition prepared in Examples 1-8 and Comparative Examples 1-10 is investigated, including the following aspects:

[0086] 1. Ultraviolet absorption capacity

[0087] Take 1g of the sample and evenly smear it on 3M tape, place it in a 37℃ constant temperature box for 20 minutes, take it out and stick it on the light-transmitting side of the quartz cuvette, and use a UV spectrophotometer to detect the UV absorption value in the 280-400nm band. Obtain a UV absorption value every 10nm UV wavelength; the UV absorption value of a certain band is the arithmetic average UV absorption value of each point in the band; record the UVB absorbance of the product, and the obtained data is shown in Table 3.

[0088] 2. Anti-inflammatory effect

[0089] Plating: RAW264.7 cells in the logarithmic growth phase were inoculated in 24-well plates at a cell density of 2*10 5 / mL, 0.5mL per well, mark the top cover of the cell plate after laying the plate, and place it in a carbon dioxide incubator for incubation for 24h;

[0090] Sample treatment: After incubating the cells for 24 hours, the supernatant was removed and 0.5 mL of the following samples were added respectively: ① blank control group: DMEM medium; ② LPS inflammation model group: LPS (1 μg / mL); ③ positive control group: 0.001% dexamethasone + LPS (1 μg / mL); ④ experimental group: 2% embodiment or comparative example + LPS (1 μg / mL);

[0091] Detection of inflammatory factors in cell supernatant: After the sample was treated with cells for 24 hours, the supernatant was aspirated into a centrifuge tube with a pipette, centrifuged at 1000r / min for 5 minutes, the precipitate was discarded and the supernatant was aspirated, and the change in the amount of inflammatory factors in the supernatant was detected using the Xinbosheng TNF-α Inflammatory Factor Detection Kit; the inhibition rate of TNF-α was calculated, TNF-α inhibition rate = (TNF-α amount in the model group - TNF-α amount in the experimental group) / TNF-α amount in the model group * 100%;

[0092] The obtained data are shown in Table 3.

[0093] Table 3

[0094]

[0095]

[0096] As can be seen from Table 3, the plant sunscreen synergistic composition provided by the present invention has good ultraviolet absorption and can also effectively resist inflammation; specifically, the UVB absorbance of the obtained product is above 1.703, and the TNF-α inhibition rate is above 36.62%;

[0097] It can be seen from Examples 1-4 and Comparative Examples 1-2 that the mass of the components has a significant effect on the performance of the product. When the mass of the components in Comparative Examples 1-2 is not within the range given in the present invention, the comprehensive performance of the obtained product is poor.

[0098] It can be seen from Example 1 and Comparative Examples 3-6 that the components in the plant sunscreen synergistic composition are indispensable, and when any one of the components is missing, an excellent comprehensive effect cannot be achieved; it can be seen from Example 1 and Comparative Examples 7-10 that when other similar ingredients are used to replace the components in the present invention, the comprehensive performance of the obtained products also shows a significant downward trend.

[0099] Effect Example 2

[0100] The effects of the present invention are as follows: The performance of the sunscreen lotions prepared by application examples 1-12, comparative application examples 1-10 and blank application examples are explored, including the following aspects:

[0101] 1. SPF value test

[0102] The test was conducted in accordance with the requirements of the "Technical Specifications for Safety of Cosmetics" (2015 edition). 115 volunteers aged 32 to 60 were selected and divided into 23 groups, with 5 people in each group. The minimum erythema dose (MED) of the subjects' skin to ultraviolet radiation was predicted and the ultraviolet radiation dose was adjusted. A spot no less than 30 cm was selected on the back of the subject. 2 Normal skin area, (2.00±0.05)mg / cm 2 The test object is evenly applied to the above area with the dosage of 1000ml, and then the irradiation dose is selected according to the specification requirements. The irradiation is carried out in three cases: ① The test object is not applied to the test object; ② The control product (SPF value 16.1±2.4, prepared according to the standard formula of high SPF standard product P2 in the 2015 edition of the "Technical Specifications for Safety of Cosmetics") is applied; ③ The test object is applied. Observe the experimental results after 24 hours, and record the MED values ​​in the three cases respectively.

[0103] SPF value calculation method: The SPF value of the test object or blank control protecting a single subject is expressed as follows:

[0104] SPFi = MED value of protected skin / MED value of unprotected skin;

[0105] Calculate the arithmetic mean of the SPF values ​​of all subjects protected by the tested object, and retain one decimal place; the obtained data are shown in Table 4.

[0106] 2. Light stability test

[0107] The sample to be tested was placed under a solar simulator for 2 hours with a radiation intensity of 650 W / m 2 , test the SPF value according to the above method. Calculate the SPF value retention rate of each sample according to the following formula based on the SPF value before and after radiation to measure the photostability of sunscreen products.

[0108] SPF value retention rate = (SPF 辐照后 / SPF 辐照前 )×100%.

[0109] The results are shown in Table 4.

[0110] 3. TEWL and a* value improvement test

[0111] Select a number of volunteers aged 18-45 who have been exposed to ultraviolet light. No skin care products were applied on the day of the visit. After washing their faces, the subjects sat quietly in a constant temperature and humidity room at 21±1℃ and 50±10% for 30 minutes. Use Visia 7 to take facial photos, perform image analysis on the exported red zone images, and obtain the a* value; use the skin transepidermal water loss probe to measure the skin transepidermal water loss. TM Hex (Courage+Khazaka) tests the initial facial TEWL value. 115 subjects with an initial facial a* value of more than 15 were screened and divided into 23 groups, with 5 people in each group. Every day when going out, they randomly used the sunscreen products in the application example on their entire face. TEWL tests and Visia 7 photography were performed on the 7th and 14th days. The improvement of each volunteer's TEWL and a* value on days 0, 7, and 14 was analyzed. The improvement is represented by the improvement rate of each value, and the calculation method is as follows:

[0112] Improvement rate relative to the initial value = |(X 使用后 -X 使用前 ) / X 使用前 ×100%|; The results are shown in Table 4.

[0113] Table 4

[0114]

[0115]

[0116] As can be seen from Table 4, the sunscreen lotion provided by the present invention has high sunscreen ability and sunscreen durability, and also has excellent after-sun repair effect; specifically, the SPF value of the obtained product is above 42.2, the SPF value retention rate after radiation is above 85.9%, the improvement rate of TEWL after 14 days of use is above 12.11%, and the a* value improvement rate is above 13.28%;

[0117] It can be seen from Application Examples 1-4 and Comparative Application Examples 1-2 that the mass fraction of the components in the plant sunscreen synergistic composition has a significant effect on the performance of the product. When the mass fraction of the components in Comparative Application Examples 1-2 is not within the range given in the present invention, the comprehensive effect of the obtained product is significantly reduced.

[0118] It can be seen from Application Example 1 and Comparative Application Examples 3-6 that the components in the plant sunscreen synergistic composition are indispensable; it can be seen from Application Example 1 and Comparative Application Examples 7-10 that when other similar ingredients are used to replace the components in the present invention, the comprehensive performance of the obtained products shows a significant downward trend;

[0119] It can be seen from Application Example 1 and Application Examples 5-8 that when the preparation method of the components in the plant sunscreen synergistic composition or the content of the effective ingredients is changed, the comprehensive performance of the obtained product will also change significantly; it can be seen from Application Example 1 and Application Examples 11-13 that when the type of physical sunscreen or chemical sunscreen in the sunscreen lotion is changed, the sunscreen ability and sunscreen durability of the obtained product will also change;

[0120] Among them, the pictures of volunteers who used the products in Application Example 1, Comparative Application Example 1 and Blank Application Example before and after use are as follows: Figure 1-3 As shown, from Figure 1-3 It can be seen that the product prepared by adopting the technical solution of the present invention has excellent effects of improving TEWL value and a* value.

[0121] Effect Example 3

[0122] The effect example of the present invention explores the use feeling scoring of the sunscreen lotion prepared in Application Example 1, which specifically includes the following steps:

[0123] Thirty Asian subjects with sensitive facial skin and recent sensitive skin symptoms were selected to participate in the test. Sunscreen products were distributed to them and each subject was given a 2mg / cm 2 Appropriate amount of the product was applied on the face for 4 weeks for sensitive skin suitability test. Consumers completed a questionnaire survey on the improvement of indicators such as feeling of use, discomfort, tolerance, mildness and non-irritation, anti-sweat effect, sun protection effect, and whether it is easy to wash. The results are shown in Table 5.

[0124] Table 5

[0125]

[0126] It can be seen from Table 5 that the product provided by the present invention has a high consumer usage score, has a mild and non-irritating effect, and is suitable for people with sensitive skin.

[0127] Finally, it should be noted that the above embodiments are intended to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A plant sunscreen synergistic composition, characterized in that: The plant sunscreen synergistic composition comprises the following components in parts by weight: 0.1-1 parts of Cimicifuga extract, 0.3-1 parts of Eucommia leaf extract, 1-3 parts of Rambutan peel extract, and 5-10 parts of Cyperus rotundus root extract.

2. The plant sunscreen synergistic composition according to claim 1, characterized in that: The plant sunscreen synergistic composition comprises the following components in parts by weight: 0.5-0.6 parts of Cimicifuga extract, 0.4-0.5 parts of Eucommia leaf extract, 1.5-2 parts of Rambutan peel extract, and 7-8 parts of Cyperus rotundus root extract.

3. The plant sunscreen synergistic composition according to claim 1, characterized in that: In the plant sunscreen synergistic composition, the mass percentage of the rambutan peel extract is 11-22%.

4. The plant sunscreen synergistic composition according to claim 1, characterized in that: The mass percentage of chlorogenic acid in the eucommia leaf extract is ≥5%.

5. The plant sunscreen synergistic composition according to claim 1, characterized in that: The preparation method of the Cimicifuga extract comprises the following steps: adding Cimicifuga to an ethanol aqueous solution for microwave extraction, filtering after the extraction is completed, collecting the filtrate, concentrating it, and drying it to obtain the Cimicifuga extract; And / or, the preparation method of the Cyperus rotundus root extract comprises the following steps: taking the Cyperus rotundus root, crushing it, adding it into an ethanol aqueous solution, heating it for extraction, filtering and concentrating it after the extraction is completed, so as to obtain the Cyperus rotundus root extract.

6. The plant sunscreen synergistic composition according to claim 5, characterized in that: Satisfy at least one of the following: a. In the method for preparing the cimicifuga extract, the mass percentage of ethanol in the ethanol aqueous solution is 60-80%, and the mass volume ratio of cimicifuga and the ethanol aqueous solution is 1g: (15-25) mL; b. In the method for preparing the Cimicifuga extract, the microwave extraction power is 380-390W and the time is 80-100s; c. In the method for preparing the Cyperus rotundus root extract, the mass percentage of ethanol in the ethanol aqueous solution is 45-55%, and the mass volume ratio of Cyperus rotundus root and the ethanol aqueous solution is 1g: (3-7) mL; d. In the preparation method of the Cyperus rotundus root extract, the heating extraction temperature is 65-75°C and the time is 1-2h.

7. Use of the plant sunscreen synergistic composition according to any one of claims 1 to 6 in the preparation of sunscreen products.

8. A sunscreen lotion, characterized in that: The sunscreen lotion comprises the following components in percentage by mass: 10-15% of the plant sunscreen synergistic composition according to any one of claims 1 to 6, 5-7% of a physical sunscreen, 10-15% of a chemical sunscreen, 10-15% of an emollient, 2-3% of a film former, 5-12% of a humectant, 0.1-0.5% of a thickener, 0.5-2% of a preservative, 1-5% of an emulsifier, and the balance is water.

9. The sunscreen lotion according to claim 8, characterized in that: The physical sunscreens include zinc oxide and titanium dioxide; the chemical sunscreens include diethylaminohydroxybenzoyl hexyl benzoate, ethylhexyl methoxycinnamate, bis-ethylhexyloxyphenol methoxyphenyl triazine and ethylhexyl triazone.

10. The sunscreen lotion according to claim 8, characterized in that: At least one of the following is met: e. The emollient comprises at least one of dicaprylyl carbonate and diisopropyl sebacate; f. The film-forming agent comprises at least one of trimethylsiloxysilicate and cyclopentasiloxane; g. The humectant comprises at least one of glycerol, 1,3-butylene glycol and 1,2-pentanediol; h. The thickener comprises at least one of xanthan gum, carbomer and sodium polyacrylate; i. The preservative comprises at least one of p-hydroxyacetophenone and hexylene glycol; j. The emulsifier includes at least one of polyglyceryl-10 oleate, polysorbate 60, and polyglyceryl-3 diisostearate.

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