Hermetia illucens sand organic fertilizer rich in benzoic acid and indole-3-acetic acid and preparation method thereof

Abstract

The invention provides a hermetia illucens sand organic fertilizer containing benzoic acid and indole-3-acetic acid and a preparation method of the hermetia illucens sand organic fertilizer. According to the method, the kitchen waste is taken as a treatment object, the recycling conversion of the hermetia illucens larvae is taken as a main technical mode, and the addition of bacillus subtilis bacterial liquid and animal viscera is taken as a main auxiliary method, so that the growth and metabolism of the hermetia illucens larvae are promoted, and the worm sand organic fertilizer containing benzoic acid and indole-3-acetic acid is produced. The insect sand is a general term of residues obtained after kitchen waste is treated by hermetia illucens, and the insect sand obtained by the method is not only rich in nitrogen, phosphorus and potassium elements, but also contains benzoic acid and indole-3-acetic acid. Bacillus subtilis is a main power for decomposing protein amino acids into benzoic acid and indole-3-acetic acid. Benzoic acid has plant nutrition and bacteriostasis functions, and indole-3-acetic acid is natural plant auxin. The insect sand product obtained by the invention has better plant fertilizer efficiency and can be used for plant culture and soil improvement.

Description

Technical Field

[0001] The invention relates to a technology for resource processing of environmental solid waste, and in particular to a black soldier fly sand organic fertilizer rich in benzoic acid and indole-3-acetic acid and a preparation method thereof. Background Art

[0002] With the rapid development of my country's social economy, the quality of life of the people has been continuously improved. The country has attached more importance to the construction of ecological civilization, and the requirements for the treatment effect and treatment quality of environmental pollutants have been continuously improved. Food waste is a type of solid waste with a large output and serious pollution in urban life. If it is not handled properly, it will cause varying degrees of pollution and damage to the soil, water sources, and the atmosphere. Using black soldier flies to treat food waste is a new type of food waste resource utilization method that breaks through the traditional composting and anaerobic biogas fermentation. After the food waste is transformed by black soldier flies, black soldier fly larvae and treatment residues containing insect feces are obtained. This residue is also called insect sand. The larvae and insect sand can be used as feed and fertilizer respectively, thereby realizing the resource utilization and transformation of food waste.

[0003] In actual use, black soldier fly sand often has the phenomenon of insufficient fertilizer effect, burning and death of seedlings. This is partly because the decomposition of kitchen waste is not complete, and partly because the content of plant nutrients in the sand is insufficient. Summary of the invention

[0004] The purpose of the present invention is to propose a preparation method of black soldier fly insect sand organic fertilizer rich in benzoic acid and indole-3-acetic acid in view of the problems of insufficient fertilizer efficiency, seedling burning and seedling death of existing insect sand organic fertilizer. The method takes food waste as the treatment object, takes the resource conversion of black soldier fly larvae as the main technical method, takes the addition of bacillus subtilis liquid and animal viscera as the main auxiliary methods, promotes the deep degradation of food waste and the growth and metabolism of black soldier fly larvae, and improves the decomposition and conversion of protein substances, finally obtains the insect sand organic fertilizer rich in nitrogen, phosphorus, potassium, benzoic acid and indole-3-acetic acid, and provides a strong guarantee for the agricultural application of the insect sand organic fertilizer.

[0005] It should be noted that, in the present invention, unless otherwise specified, the specific meaning of "including" in relation to composition limitations and descriptions includes both open-ended "including", "comprising", etc. and similar meanings, as well as closed-ended "composed of", "composed of", etc. and similar meanings.

[0006] To achieve the above object, the technical solution adopted by the present invention is: a method for preparing black soldier fly sand organic fertilizer rich in benzoic acid and indole-3-acetic acid, comprising the following steps:

[0007] Step 1: Put the solid food waste into a treatment box, add Bacillus subtilis liquid to the food waste, mix well, and then add black soldier fly larvae;

[0008] Step 2: Cultivating black soldier fly larvae at room temperature;

[0009] Step 3: On the 4th to 7th day of breeding, add animal viscera and Bacillus subtilis liquid to the kitchen waste, mix well, and allow the black soldier fly larvae to continue to feed and grow;

[0010] Step 4: On the 14th to 22nd day of breeding, the breeding is stopped, the black soldier fly larvae and the insect sand are separated, and the black soldier fly insect sand organic fertilizer rich in benzoic acid and indole-3-acetic acid is obtained.

[0011] Furthermore, the solid phase food waste is obtained by boiling the food waste to separate the oil phase, the water phase and the solid phase, and the water content of the solid phase food waste is 60-90%, preferably 70-85%. Unless otherwise specified, % in the present invention refers to mass percentage.

[0012] Furthermore, in step 1, 10-30 ml of Bacillus subtilis solution is added per kilogram of food waste, preferably 10-20 ml of Bacillus subtilis solution is added per kilogram of food waste; the concentration of Bacillus subtilis in the Bacillus subtilis solution is not less than 1×10 8 The Bacillus subtilis is Bacillus subtilis CCTCCM2021184, which is deposited in the China Center for Type Culture Collection. The name of the deposited culture is Bacillus subtilis NJZ, the deposit number is CCTCCNo.M2021184, and the deposit date is March 9, 2021.

[0013] Furthermore, in step 1, 1000-4000 black soldier fly larvae are added per kilogram of food waste, preferably 2000-3000 larvae are added per kilogram of food waste, the individual size of the black soldier fly larvae is 0.01-0.04g, and preferably the individual size of the black soldier fly larvae is 0.02-0.03g.

[0014] Furthermore, the room temperature in step 2 is 20-35°C, preferably 25-32°C.

[0015] Further, in step 3, on the 4th to 7th day of cultivation, 30-100 g of animal offal and 10-30 ml of Bacillus subtilis solution are added to each kilogram of food waste. Preferably, on the 5th to 6th day of cultivation, 40-80 g of animal offal and 30-100 ml of Bacillus subtilis solution are added to each kilogram of food waste.

[0016] Furthermore, the Bacillus subtilis is Bacillus subtilis CCTCCM2021184, and the concentration of Bacillus subtilis in the Bacillus subtilis liquid is not less than 1×10 8 The Bacillus subtilis is Bacillus subtilis CCTCCM2021184, which is deposited in the China Center for Type Culture Collection, the name of the deposited culture is Bacillus subtilis NJZ, the deposit number is CCTCC No. M2021184, and the deposit date is March 9, 2021.

[0017] Furthermore, the animal offal includes but is not limited to chicken liver and / or chicken heart, preferably fresh chicken liver.

[0018] Furthermore, in step 4, the culture is stopped on the 16th to 21st day of culture.

[0019] The present invention promotes the deep degradation of kitchen waste by adding Bacillus subtilis to kitchen waste, enhances the digestion and metabolism function of black soldier fly larvae, and increases the storage content of nitrogen, phosphorus and potassium elements in the insect sand final product. At the same time, the Bacillus subtilis of the present invention also has the ability to decompose phenylalanine and tryptophan into benzoic acid and indole-3-acetic acid. Among them, benzoic acid has plant nutrition and antibacterial functions, and indole-3-acetic acid is a natural plant growth hormone. The insect sand rich in nitrogen, phosphorus, potassium, benzoic acid and indole-3-acetic acid has good plant fertilizer effect, solves the problem of insect sand organic fertilizer burning seedlings, and provides a powerful method for the agricultural application of insect sand.

[0020] Another object of the present invention is to disclose a black soldier fly insect sand organic fertilizer rich in benzoic acid and indole-3-acetic acid, which is prepared by the above-mentioned preparation method. Benzoic acid has plant nutrition, antibacterial and antiseptic effects and benzoin, and indole-3-acetic acid has the effect of promoting plant growth. The black soldier fly insect sand organic fertilizer rich in benzoic acid and indole-3-acetic acid of the present invention is a high-fertilizer quality insect sand product, which provides an effective path for improving the economic value of black soldier fly treatment products.

[0021] Furthermore, the black soldier fly sand organic fertilizer rich in benzoic acid and indole-3-acetic acid has a benzoic acid content of 1100-1500 mg / kg dry weight and an indole-3-acetic acid content of 20-90 mg / kg dry weight.

[0022] Furthermore, the black soldier fly sand organic fertilizer rich in benzoic acid and indole-3-acetic acid has a nitrogen content of 25-32 mg / g dry weight, a phosphorus content of 18-30 mg / g dry weight, and a potassium content of 35-45 mg / g dry weight.

[0023] The black soldier fly sand organic fertilizer rich in benzoic acid and indole-3-acetic acid and the preparation method thereof have the following advantages compared with the prior art:

[0024] 1) Black soldier fly larvae can convert kitchen solid waste into black soldier fly sand organic fertilizer, which contains not only nitrogen, phosphorus and potassium elements, but also benzoic acid and indole-3-acetic acid. Among them, the nitrogen content is 25-32 mg / g dry weight, the phosphorus content is 18-30 mg / g dry weight, the potassium content is 35-45 mg / g dry weight, the benzoic acid content is 1100-1500 mg / kg dry weight, and the indole-3-acetic acid content is 20-90 mg / kg dry weight. The content of nitrogen, phosphorus and potassium elements has exceeded my country's national standard for organic fertilizers NY252-2012.

[0025] 2) The Bacillus subtilis CCTCCM2021184 used in the present invention can convert phenylalanine into benzoic acid and tryptophan into organic substances such as indole and indoleacetic acid during growth and metabolism, as shown in the following formula.

[0026]

[0027] 3) The present invention adds Bacillus subtilis CCTCCM2021184 to the kitchen waste, which can not only promote the growth and development of black soldier fly larvae, but also further enhance the generation and yield of benzoic acid and indole-3-acetic acid in the black soldier fly sand. Benzoic acid and indole-3-acetic acid come from the decomposition and metabolism of phenylalanine, tyrosine and tryptophan in protein. Therefore, in the process of kitchen waste treatment, the simultaneous supplementation of Bacillus subtilis CCTCCM2021184 and protein substances (animal offal) will contribute to the production of benzoic acid, indole and indoleacetic acid sand.

[0028] 4) The black soldier fly insect sand organic fertilizer of the present invention is rich in benzoic acid and indole-3-acetic acid. Benzoic acid has an antibacterial and antiseptic effect and has a benzoin aroma, which is beneficial to the long-term preservation of the insect sand organic fertilizer. Indole-3-acetic acid has a plant growth-promoting effect. Therefore, the production of insect sand with the above aromatic substances is beneficial to improving the fertilizer quality of the insect sand.

[0029] In summary, the present invention provides a black soldier fly sand organic fertilizer rich in benzoic acid and indole-3-acetic acid and a preparation method thereof. The method uses kitchen waste as the treatment object, uses the resource conversion of black soldier fly larvae as the main technical means, and uses the addition of Bacillus subtilis liquid and animal viscera as the main auxiliary methods, and finally obtains a sand organic fertilizer product rich in benzoic acid and indole-3-acetic acid. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1The figures are the liquid chromatography detection results of the standard samples and the embodiments; wherein A is the HPLC detection spectrum of the standard samples of indole, indole-3-acetic acid, p-cresol and 3-methylindole at 288 nm; B is the HPLC detection spectrum of the insect sand obtained in Example 1 of the present invention at 288 nm; C is the HPLC detection spectrum of the standard sample of benzoic acid at 235 nm; and D is the HPLC detection spectrum of the insect sand obtained in Example 1 of the present invention at 235 nm. DETAILED DESCRIPTION

[0031] The present invention is further described below with reference to the examples. The description of the technical features described below is based on representative embodiments and specific examples of the present invention, but the present invention is not limited to these embodiments and specific examples. It should be noted that:

[0032] Unless otherwise stated, the units used in this specification are all international standard units, and the numerical values ​​and numerical ranges appearing in the present invention should be understood to include the inevitable systematic errors in industrial production.

[0033] In this specification, the numerical range expressed using "a numerical value A to a numerical value B" means a range including the endpoints numerical values ​​A and B.

[0034] In the present specification, a numerical range expressed using "above" or "below" means a numerical range including the number.

[0035] In this specification, the word "may" means both performing a certain process and not performing a certain process.

[0036] In this specification, the use of "optional" or "optional" indicates that certain substances, components, execution steps, application conditions and other factors are used or not used.

[0037] In this specification, when "normal temperature" or "room temperature" is used, the temperature may be 15-25°C.

[0038] In this manual, the reagents or instruments used without indicating the manufacturer are all conventional products that can be obtained through commercial purchase.

[0039] Example 1

[0040] This embodiment discloses a black soldier fly sand organic fertilizer rich in benzoic acid and indole-3-acetic acid and a preparation method thereof, which is characterized by being implemented by the following steps:

[0041] Step 1: The kitchen waste is separated into three phases: oil phase, water phase and solid phase by boiling. The solid phase kitchen waste is put into the treatment box. 10 ml of 3.8×10 81000 larvae per kilogram of food waste are added to the mixture, and the individual size of the larvae reaches 0.01-0.02 g.

[0042] Step 2: Maintain the room temperature during the breeding process at 20-25°C, allowing the black soldier fly larvae to feed on food waste and grow;

[0043] Step 3 On the fourth day of culture, add 30 g of fresh chicken liver and 30 ml of 4.6×10 8 100g / L of Bacillus subtilis CCTCCM2021184 bacterial solution, mix evenly, and let the black soldier fly larvae continue to feed and grow;

[0044] Step 4: On the 14th day of cultivation, the treatment is stopped, the black soldier fly larvae and the insect sand are separated, and the black soldier fly insect sand organic fertilizer rich in benzoic acid and indole-3-acetic acid is obtained.

[0045] After testing, the obtained black soldier fly sand organic fertilizer rich in benzoic acid and indole-3-acetic acid had a nitrogen content of 27.2 mg / g dry weight, a phosphorus content of 19.6 mg / g dry weight, a potassium content of 36.7 mg / g dry weight, a benzoic acid content of 987.6 mg / kg dry weight, and an indole-3-acetic acid content of 22.3 mg / kg dry weight.

[0046] Example 2

[0047] This embodiment discloses a black soldier fly sand organic fertilizer rich in benzoic acid and indole-3-acetic acid and a preparation method thereof, which is characterized by being implemented by the following steps:

[0048] Step 1: The kitchen waste is separated into three phases: oil phase, water phase and solid phase by boiling. The solid phase kitchen waste is put into the treatment box. 20 ml of 3.8×10 8 100 g / L of Bacillus subtilis CCTCCM2021184 bacterial solution was mixed evenly, and then black soldier fly larvae were added at a ratio of 2,000 larvae per kilogram of food waste, and the individual size of the larvae reached 0.02-0.04 g;

[0049] Step 2: Maintain the room temperature during the breeding process at 25-35°C, allowing the black soldier fly larvae to feed on food waste and grow;

[0050] Step 3: On the fifth day of culture, add 60 g of fresh chicken hearts and 50 ml of 4.6×10 8 100g / L of Bacillus subtilis CCTCCM2021184 bacterial solution, mix evenly, and let the black soldier fly larvae continue to feed and grow;

[0051] Step 4: On the 20th day of cultivation, the treatment is stopped, the black soldier fly larvae and the insect sand are separated, and the black soldier fly insect sand organic fertilizer rich in benzoic acid and indole-3-acetic acid is obtained.

[0052] After testing, the obtained black soldier fly sand organic fertilizer rich in benzoic acid and indole-3-acetic acid had a nitrogen content of 29.1 mg / g dry weight, a phosphorus content of 25.2 mg / g dry weight, a potassium content of 37.9 mg / g dry weight, a benzoic acid content of 1145.3 mg / kg, and an indole-3-acetic acid content of 43.9 mg / kg.

[0053] Example 3

[0054] This embodiment discloses a black soldier fly sand organic fertilizer rich in benzoic acid and indole-3-acetic acid and a preparation method thereof, which is characterized by being implemented by the following steps:

[0055] Step 1: The kitchen waste is separated into three phases: oil phase, water phase and solid phase by boiling. The solid phase kitchen waste is put into the treatment box. 30 ml of 3.8×10 8 100 g / L of Bacillus subtilis CCTCCM2021184 bacterial solution was mixed evenly, and then black soldier fly larvae were added at a ratio of 4,000 larvae per kilogram of food waste, and the individual size of the larvae reached 0.02-0.04 g;

[0056] Step 2: Maintain the room temperature during the breeding process at 27-35°C, allowing the black soldier fly larvae to feed on the food waste and grow;

[0057] Step 3 On the 7th day of culture, add 100 g of fresh chicken liver and 100 ml of 3.1×10 8 100g / L of Bacillus subtilis CCTCCM2021184 bacterial solution, mix evenly, and let the black soldier fly larvae continue to feed and grow;

[0058] Step 4: On the 22nd day of the culture, the treatment is stopped, the black soldier fly larvae and the insect sand are separated, and the black soldier fly insect sand organic fertilizer rich in benzoic acid and indole-3-acetic acid is obtained.

[0059] After testing, the obtained black soldier fly sand organic fertilizer rich in benzoic acid and indole-3-acetic acid had a nitrogen content of 30.1 mg / g dry weight, a phosphorus content of 27.7 mg / g dry weight, a potassium content of 43.1 mg / g dry weight, a benzoic acid content of 1338.8 mg / kg, and an indole-3-acetic acid content of 86.4 mg / kg.

[0060] Comparison Example

[0061] This comparative example discloses a black soldier fly sand organic fertilizer and a preparation method thereof, which is characterized by being implemented by the following steps:

[0062] Step 1: Boil the food waste to separate the oil phase, water phase and solid phase, take the solid food waste and put it into the treatment box, add 30 ml of water per kilogram of food waste, mix well, and then add black soldier fly larvae at a ratio of 4000 larvae per kilogram of food waste, and the individual size of the larvae reaches 0.02-0.04g;

[0063] Step 2: Maintain the room temperature during the breeding process at 27-35°C, allowing the black soldier fly larvae to feed on the food waste and grow;

[0064] Step 3: On the 7th day of the culture, 100 ml of water is added to each kilogram of the food waste in step 1, and the mixture is mixed evenly, so that the black soldier fly larvae continue to feed and grow;

[0065] Step 4: On the 22nd day of the culture, the treatment is stopped, the black soldier fly larvae and the insect sand are separated, and the black soldier fly insect sand organic fertilizer is obtained.

[0066] After testing, the obtained black soldier fly sand organic fertilizer had a nitrogen content of 18.3 mg / g dry weight, a phosphorus content of 10.8 mg / g dry weight, a potassium content of 19.9 mg / g dry weight, a benzoic acid content of 0 mg / kg dry weight, and an indole-3-acetic acid content of 0 mg / kg dry weight.

[0067] The control example shows that under the treatment conditions without adding Bacillus subtilis CCTCCM2021184 bacterial solution and animal viscera, the black soldier fly sand does not contain benzoic acid and indole-3-acetic acid, and the content of nitrogen, phosphorus and potassium elements is also low, lower than that of Examples 1, 2, and 3 using bacterial solution and animal viscera.

[0068] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for preparing a black soldier fly sand organic fertilizer rich in benzoic acid and indole-3-acetic acid, characterized in that: The following steps are involved: Step 1: Put the solid food waste into a treatment box, add Bacillus subtilis liquid to the food waste, mix well, and then add black soldier fly larvae; Step 2: Cultivating black soldier fly larvae at room temperature; Step 3: On the 4th to 7th day of breeding, add animal viscera and Bacillus subtilis liquid to the kitchen waste, mix well, and allow the black soldier fly larvae to continue to feed and grow; Step 4: On the 14th to 22nd day of breeding, the breeding is stopped, the black soldier fly larvae and the insect sand are separated, and the black soldier fly insect sand organic fertilizer rich in benzoic acid and indole-3-acetic acid is obtained.

2. The method for preparing the black soldier fly sand organic fertilizer rich in benzoic acid and indole-3-acetic acid according to claim 1, characterized in that: The solid-phase food waste is obtained by boiling the food waste and performing three-phase separation of oil phase, water phase and solid phase.

3. The method for preparing the black soldier fly sand organic fertilizer rich in benzoic acid and indole-3-acetic acid according to claim 1, characterized in that: Step 1: add 10-30 ml of Bacillus subtilis solution per kilogram of food waste; the Bacillus subtilis is Bacillus subtilis CCTCCM2021184, and the concentration of Bacillus subtilis in the Bacillus subtilis solution is not less than 1×10 8 Pieces / liter.

4. The method for preparing the black soldier fly sand organic fertilizer rich in benzoic acid and indole-3-acetic acid according to claim 1, characterized in that: Step 1: adding 1000-4000 black soldier fly larvae per kilogram of food waste, wherein the individual size of the black soldier fly larvae is 0.01-0.04g.

5. The method for preparing the black soldier fly sand organic fertilizer rich in benzoic acid and indole-3-acetic acid according to claim 1, characterized in that: The room temperature in step 2 is 20-35°C.

6. The method for preparing the black soldier fly sand organic fertilizer rich in benzoic acid and indole-3-acetic acid according to claim 1, characterized in that: Step 3: On the 4th to 7th day of cultivation, 30-100 g of animal viscera and 30-100 ml of Bacillus subtilis liquid are added to each kilogram of food waste; And / or, the Bacillus subtilis is Bacillus subtilis CCTCCM2021184, and the concentration of Bacillus subtilis in the Bacillus subtilis liquid is not less than 1×10 8 Pieces / liter; And / or, the animal offal is chicken liver and / or chicken heart.

7. The method for preparing the black soldier fly sand organic fertilizer rich in benzoic acid and indole-3-acetic acid according to claim 1, characterized in that: Step 4: Stop the culture on the 16th to 21st day of culture.

8. A black soldier fly sand organic fertilizer rich in benzoic acid and indole-3-acetic acid, characterized in that: The invention is prepared by the preparation method according to any one of claims 1 to 7.

9. The black soldier fly sand organic fertilizer rich in benzoic acid and indole-3-acetic acid according to claim 8, characterized in that: The benzoic acid content is 1100-1500 mg / kg dry weight, and the indole-3-acetic acid content is 20-90 mg / kg dry weight.

10. The black soldier fly sand organic fertilizer rich in benzoic acid and indole-3-acetic acid according to claim 8, characterized in that: The nitrogen content is 25-32 mg / g dry weight, the phosphorus content is 18-30 mg / g dry weight, and the potassium content is 35-45 mg / g dry weight.

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