Centrifugal micro-fluidic chip for knee joint motor injury gene detection

Abstract

The embodiment of the invention discloses a centrifugal micro-fluidic chip for knee joint motor injury gene detection. The centrifugal micro-fluidic chip is characterized in that one end of a first mixing chamber is connected with a first reaction chamber, one end of a second mixing chamber is connected with the first reaction chamber, a second reagent storage chamber is connected with the second mixing chamber, and a third reagent storage chamber is connected with the second mixing chamber; one end of the first reaction chamber is connected with one end of the first liquid buffer chamber, the other end of the first liquid buffer chamber is connected with the head end of the first fluid shunting runner, and the other ends of the second preset number of first liquid quantification chambers are connected with the second preset number of amplification detection chambers. Therefore, the centrifugal micro-fluidic chip for knee joint motor injury gene detection can shorten the steps of gene detection, shorten the time consumption of gene detection, do not need to consume a large number of knee joint motor injury gene detection kits, and save detection resources.

Description

Technical Field

[0001] The embodiments of the present disclosure relate to the field of medical device technology, and specifically to a centrifugal microfluidic chip for genetic detection of sports injuries of the knee joint. Background Art

[0002] Knee sports injuries are related to many factors, among which genetic factors are an important factor. Some gene mutations are related to the risk of knee sports injuries. Therefore, through genetic testing, people susceptible to knee sports injuries can be screened and prevented, or personalized treatment and rehabilitation plans can be formulated for patients who have already suffered injuries. At present, genetic testing is usually done by using polymerase chain reaction (PCR) technology or by preparing a knee sports injury gene detection kit to detect whether there are susceptibility gene sites related to knee sports injury genes in the sample.

[0003] However, in practice, it is found that when the above method is used to detect whether there are susceptibility gene loci related to knee sports injury genes in samples, the following technical problems often occur:

[0004] When using polymerase chain reaction technology for testing, polymerase chain reaction technology has high requirements for instruments and equipment and high requirements for testing skills of testers, and requires cumbersome testing steps, resulting in greater difficulty in testing and longer testing time; when using knee sports injury gene testing kits for testing, each knee sports injury gene testing kit detects fewer gene loci, and the number of susceptibility gene loci of knee sports injury genes is large, resulting in more tests required and more knee sports injury gene testing kits used, resulting in longer testing time and more consumption of knee sports injury gene testing kits.

[0005] The above information disclosed in this Background section is only for enhancement of understanding of the background of the inventive concept and therefore it may contain information that does not form the prior art that is already known in this country to a person of ordinary skill in the art. Summary of the invention

[0006] The content of this disclosure is used to introduce concepts in a brief form, which will be described in detail in the detailed implementation section below. The content of this disclosure is not intended to identify the key features or essential features of the technical solution claimed for protection, nor is it intended to limit the scope of the technical solution claimed for protection.

[0007] Some embodiments of the present disclosure propose a centrifugal microfluidic chip for genetic detection of knee sports injuries to solve one or more of the technical problems mentioned in the above background technology section.

[0008] In a first aspect, some embodiments of the present disclosure provide a centrifugal microfluidic chip for genetic detection of knee sports injuries, characterized in that the centrifugal microfluidic chip comprises a plastic substrate, various flow channels, a target capillary valve and a second preset number of first capillary valves, wherein the plastic substrate comprises a first reaction chamber, a first mixing chamber, a first reagent storage chamber, a sample injection chamber, a second preset number of first liquid quantitative chambers, a second reagent storage chamber, a first liquid buffer chamber, a second mixing chamber, a third reagent storage chamber and a second preset number of amplification detection chambers; the various flow channels comprise a first preset number of first capillary flow channels, a second capillary flow channel, a first siphon flow channel, a second siphon flow channel, a first fluid diversion flow channel, a third capillary flow channel, a quantitative buffer flow channel, a first connecting flow channel and the second preset number of second connecting flow channels; the sample injection chamber is connected to the first mixing chamber via the second capillary flow channel, the first reagent storage chamber is connected to the first mixing chamber via the first preset number of first capillary flow channels, and the first mixing chamber is connected to the first mixing chamber via the first preset number of first capillary flow channels. One end of the mixing chamber is connected to the first reaction chamber through the first siphon flow channel, one end of the second mixing chamber is connected to the first reaction chamber through the second siphon flow channel, the second reagent storage chamber is connected to the second mixing chamber through the first connecting flow channel, the third reagent storage chamber is connected to the second mixing chamber through the target capillary valve, one end of the first reaction chamber is connected to one end of the first liquid buffer chamber through the quantitative buffer flow channel, the other end of the first liquid buffer chamber is connected to the head end of the first fluid diversion flow channel through the third capillary flow channel, the first fluid diversion flow channel is provided with the second preset number of liquid diversion ports, the second preset number of liquid diversion ports are connected to one end of the second preset number of first liquid quantitative chambers through the second preset number of second connecting flow channels, and the other end of the second preset number of first liquid quantitative chambers is connected to the second preset number of amplification detection chambers through the second preset number of first capillary valves.

[0009] Optionally, at least one first mixing column is disposed in the first reagent storage chamber, and at least one first spoiler column and at least one second spoiler column are disposed in the first reaction chamber.

[0010] Optionally, a first spoiler plate is disposed in the first mixing chamber, and at least one third spoiler column is disposed in the second mixing chamber.

[0011] Optionally, at least two sample injection holes are provided on the sample injection chamber, the first reagent storage chamber, the second reagent storage chamber, and the third reagent storage chamber.

[0012] Optionally, the centrifugal microfluidic chip for genetic detection of knee sports injuries further comprises a first waste liquid chamber, which is connected to the end of the first fluid diversion channel, and is filled with a water-absorbing material.

[0013] Optionally, each amplification detection chamber in the second preset number of amplification detection chambers includes a probe and a primer, and the probe and primer included in each amplification detection chamber in the second preset number of amplification detection chambers are different.

[0014] Optionally, the distance between each two adjacent amplification detection chambers in the second preset number of amplification detection chambers is the same.

[0015] Optionally, the first reaction chamber, the first mixing chamber, the first reagent storage chamber, the sample injection chamber, the second preset number of first liquid quantitative chambers, the first waste liquid chamber, the second reagent storage chamber, the first liquid buffer chamber, the second mixing chamber, the third reagent storage chamber and the second preset number of amplification detection chambers are diffusely distributed at a preset circle center position, the first reagent storage chamber, the sample injection chamber, the third reagent storage chamber and the second reagent storage chamber are located in the innermost circumferential area of ​​the centrifugal microfluidic chip for genetic detection of knee sports injuries, and the first mixing chamber and the second mixing chamber are located in the second layer of the centrifugal microfluidic chip for genetic detection of knee sports injuries. The circumferential area, the first reaction chamber and the first liquid buffer chamber are located in the third circumferential area of ​​the centrifugal microfluidic chip for genetic detection of knee sports injuries, the second preset number of first liquid quantitative chambers are located in the fourth circumferential area of ​​the centrifugal microfluidic chip for genetic detection of knee sports injuries, and the second preset number of amplification detection chambers are located in the outermost circumferential area of ​​the centrifugal microfluidic chip for genetic detection of knee sports injuries. When in use, the centrifugal microfluidic chip for genetic detection of knee sports injuries is mounted on a carrier. When the carrier is running, the carrier drives the centrifugal microfluidic chip for genetic detection of knee sports injuries to rotate clockwise or counterclockwise with the preset center position as the reference.

[0016] Optionally, the center of the centrifugal microfluidic chip for genetic detection of knee sports injuries is a hollow structure, the hollow structure is connected to the first fluid diversion channel through a main ventilation channel, and the other end of the first mixing chamber, the other end of the second mixing chamber, and the first liquid buffer chamber are connected to the main ventilation channel through a sub-ventilation channel.

[0017] In a second aspect, some embodiments of the present disclosure provide an electronic device comprising: one or more processors; a storage device on which one or more programs are stored, and when the one or more programs are executed by the one or more processors, the one or more processors implement the method described in any implementation manner in the above-mentioned first aspect.

[0018] In a third aspect, some embodiments of the present disclosure provide a computer-readable medium having a computer program stored thereon, wherein when the computer program is executed by a processor, the method described in any one of the implementations of the first aspect or the second aspect is implemented.

[0019] The above-mentioned various embodiments of the present disclosure have the following beneficial effects: through a centrifugal microfluidic chip for knee sports injury gene detection according to some embodiments of the present disclosure, the difficulty of detection can be reduced, the time consumption of detection can be shortened, and the situation of consuming a large number of knee sports injury gene detection kits can be reduced. The reasons for the difficulty of detection, the long detection time, and the large number of knee sports injury gene detection kits are: when using polymerase chain reaction technology for detection, the polymerase chain reaction technology has high requirements for instruments and equipment and the detection skills of detection personnel, and requires cumbersome detection steps, resulting in greater difficulty in detection and longer detection time; when using a knee sports injury gene detection kit for detection, each knee sports injury gene detection kit detects fewer gene sites, and the number of susceptible gene sites of knee sports injury genes is large, resulting in more times of detection, more knee sports injury gene detection kits used, resulting in longer detection time and more consumption of knee sports injury gene detection kits.Based on this, some embodiments of the present disclosure are centrifugal microfluidic chips for genetic detection of knee sports injuries, characterized in that the centrifugal microfluidic chip includes a plastic substrate, various flow channels, a target capillary valve and a second preset number of first capillary valves, wherein the plastic substrate includes a first reaction chamber, a first mixing chamber, a first reagent storage chamber, a sample injection chamber, a second preset number of first liquid quantitative chambers, a second reagent storage chamber, a first liquid buffer chamber, a second mixing chamber, a third reagent storage chamber and a second preset number of amplification detection chambers; the various flow channels include a first preset number of first capillary flow channels, a second capillary flow channel, a first siphon flow channel, a second siphon flow channel, a first fluid diversion flow channel, a third capillary flow channel, a quantitative buffer flow channel, a first connecting flow channel and the second preset number of second connecting flow channels; the sample injection chamber is connected to the first mixing chamber via the second capillary flow channel, the first reagent storage chamber is connected to the first mixing chamber via the first preset number of first capillary flow channels, and the first mixing chamber One end of the first reaction chamber is connected to the first reaction chamber through the first siphon flow channel, one end of the second mixing chamber is connected to the first reaction chamber through the second siphon flow channel, the second reagent storage chamber is connected to the second mixing chamber through the first connecting flow channel, the third reagent storage chamber is connected to the second mixing chamber through the target capillary valve, one end of the first reaction chamber is connected to one end of the first liquid buffer chamber through the quantitative buffer flow channel, the other end of the first liquid buffer chamber is connected to the head end of the first fluid diversion flow channel through the third capillary flow channel, the first fluid diversion flow channel is provided with the second preset number of liquid diversion ports, the second preset number of liquid diversion ports are connected to one end of the second preset number of first liquid quantitative chambers through the second preset number of second connecting flow channels, and the other end of the second preset number of first liquid quantitative chambers is connected to the second preset number of amplification detection chambers through the second preset number of first capillary valves. Also, only the centrifugal microfluidic chip for knee sports injury gene detection can be used to detect whether there are susceptibility gene sites related to knee sports injury genes in the sample, without the need for higher instruments and equipment for detection. Therefore, the difficulty of sample detection can be reduced. Also, only the sample and the detection reagent need to be injected into the centrifugal microfluidic chip for knee sports injury gene detection to automatically detect the sample and the reagent, thereby lowering the requirements for the detection skills of the detection personnel. Also, because the centrifugal microfluidic chip contains the second preset number of amplification detection chambers, the second number can be 63, thereby, multiple existing knee sports injury gene sites can be detected simultaneously, rather than multiple detections by using multiple knee sports injury gene detection kits.Therefore, the centrifugal microfluidic chip for genetic testing of knee sports injuries can shorten the steps of genetic testing, shorten the time of genetic testing, and eliminate the need to consume a large number of genetic testing kits for knee sports injuries, thereby saving testing resources. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] The above and other features, advantages and aspects of the embodiments of the present disclosure will become more apparent with reference to the following detailed description in conjunction with the accompanying drawings. Throughout the accompanying drawings, the same or similar reference numerals represent the same or similar elements. It should be understood that the drawings are schematic and that components and elements are not necessarily drawn to scale.

[0021] Figure 1 It is a schematic diagram of the structure of the hollow cavity structure and each flow channel in the centrifugal microfluidic chip for gene detection of sports injuries of knee joint according to the present disclosure;

[0022] Figure 2 It is a schematic diagram of the structure of some embodiments of the centrifugal microfluidic chip for gene detection of sports injuries of knee joint according to the present disclosure;

[0023] Figure 3 It is a structural schematic diagram of a centrifugal microfluidic chip placement platform for gene detection of knee sports injuries according to the present disclosure;

[0024] Figure 4 It is a schematic diagram of the structure of an electronic device suitable for implementing some embodiments of the present disclosure. DETAILED DESCRIPTION

[0025] Embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings. Although certain embodiments of the present disclosure are shown in the accompanying drawings, it should be understood that the present disclosure can be implemented in various forms and should not be construed as being limited to the embodiments set forth herein. On the contrary, these embodiments are provided to provide a more thorough and complete understanding of the present disclosure. It should be understood that the drawings and embodiments of the present disclosure are only for exemplary purposes and are not intended to limit the scope of protection of the present disclosure.

[0026] It should also be noted that, for ease of description, only the parts related to the invention are shown in the drawings. In the absence of conflict, the embodiments and features in the embodiments of the present disclosure can be combined with each other.

[0027] It should be noted that the concepts such as "first" and "second" mentioned in the present disclosure are only used to distinguish different devices, modules or units, and are not used to limit the order or interdependence of the functions performed by these devices, modules or units.

[0028] It should be noted that the modifications of "one" and "plurality" mentioned in the present disclosure are illustrative rather than restrictive, and those skilled in the art should understand that unless otherwise clearly indicated in the context, it should be understood as "one or more".

[0029] The names of the messages or information exchanged between multiple devices in the embodiments of the present disclosure are only used for illustrative purposes and are not used to limit the scope of these messages or information.

[0030] The present disclosure will be described in detail below with reference to the accompanying drawings and in conjunction with embodiments.

[0031] Figure 1 A structural diagram of a centrifugal microfluidic chip for genetic detection of knee sports injuries according to the present disclosure is shown.

[0032] like Figure 1 As shown, the centrifugal microfluidic chip for genetic detection of knee sports injuries may include a first reaction chamber 1, a first mixing chamber 2, a first reagent storage chamber 3, a first mixing column 4, a first capillary channel 5, a first spoiler 6, a first spoiler column 7, a second spoiler column 8, a sample injection chamber 9, a second capillary channel 10, a first siphon channel 11, a second siphon channel 12, a first fluid diversion channel 13, a first liquid quantitative chamber 14, a first capillary valve 15, a water-absorbing material 16, a first waste liquid chamber 17, a third capillary channel 18, a second reagent storage chamber 19, a first liquid buffer chamber 20, a second mixing chamber 21, a third spoiler column 22, a third reagent storage chamber 23, a quantitative buffer channel 24, and an amplification detection chamber 25.

[0033] In some embodiments, the centrifugal microfluidic chip includes a plastic substrate 01, various flow channels, a target capillary valve, and a second preset number of first capillary valves 15. The shape of the plastic substrate 01 may be circular. The material of the plastic substrate 01 may include, but is not limited to, polymethyl methacrylate (PMMA), polycarbonate (PC), polystyrene (PS), and polypropylene (PP). The materials of the first reaction chamber 1, the first mixing chamber 2, the first reagent storage chamber 3, the sample injection chamber 9, the second preset number of first liquid quantitative chambers 14, the first waste liquid chamber 17, the second reagent storage chamber 19, the first liquid buffer chamber 20, the second mixing chamber 21, the third reagent storage chamber 23, and the second preset number of amplification detection chambers 25 may be the same as the material of the plastic substrate 01. The specific value of the second preset number is not limited here, and can be set according to specific experiments. For example, the second preset number can be 63. The target capillary valve and the second preset number of first capillary valves 15 may be made of nano-silicon dioxide or other materials that can make the contact angle greater than 120 degrees, or may be the same as the material of the plastic substrate 01 .

[0034] In some embodiments, the plastic substrate 01 includes a first reaction chamber 1, a first mixing chamber 2, a first reagent storage chamber 3, a sample injection chamber 9, a second preset number of first liquid quantitative chambers 14, a first waste liquid chamber 17, a second reagent storage chamber 19, a first liquid buffer chamber 20, a second mixing chamber 21, a third reagent storage chamber 23 and a second preset number of amplification detection chambers 25. Among them, the first reaction chamber 1 can be a chamber for reacting a sample solution and a lysis reagent. The first mixing chamber 2 can be a chamber for mixing a sample solution and a lysis reagent. The first reagent storage chamber 3 can be a chamber for receiving and storing an injected lysis reagent. The sample injection chamber 9 can be a chamber for receiving an injected sample solution. The amount of liquid that can be stored in each of the second preset number of first liquid quantitative chambers 14 is the same. Here, the amount of liquid that can be contained in each of the second preset number of first liquid quantitative chambers 14 is not limited and can be set according to experimental requirements. For example, the first liquid quantitative chamber 14 can contain 3 microliters of solution. The first liquid quantitative chamber 14 in the second preset number of first liquid quantitative chambers 14 may be a chamber for storing a target mixed solution obtained by mixing the first mixed solution and the second mixed solution, and a chamber for processing the target mixed solution in quantity. The first mixed solution may be a solution obtained by mixing a sample solution and a lysis reagent. The second mixed solution may be a solution obtained by mixing denucleic acid water and a reaction premix solution. The first waste liquid chamber 17 may be a chamber for storing excess target premix solution and a chamber for storing waste liquid generated by the reaction. The excess target premix solution may represent the remaining liquid after the second preset number of amplification detection chambers 25 are filled with the target premix solution. The second reagent storage chamber 19 may be a chamber for receiving and storing the injected lysis reagent. The first liquid buffer chamber 20 may be a chamber for slowing down the liquid flow rate in the first reaction chamber 1. The second mixing chamber 21 may be a chamber for mixing denucleic acid water and the reaction premix solution. The third reagent storage chamber 23 may be used to receive and store the injected reaction premix solution. The second preset number of amplification detection chambers 25 can be chambers for detecting each knee sports injury gene site. Each amplification detection chamber 25 in the second preset number of amplification detection chambers 25 can detect a gene site. It should be noted that the volume of the above-mentioned lysis reagent can be 2000 microliters, and the volume of the above-mentioned denucleic acid water can be 450 microliters. The volume of the above-mentioned reaction premix can be 750 microliters. The above-mentioned lysis reagent can include a detergent, a protease and a buffer. Here, the specific values ​​of the volume of the detergent, the volume of the protease and the volume of the appropriate buffer included in the above-mentioned lysis reagent are not limited and can be adjusted according to the experiment.The reaction premix may include enzymes, dNTPs (deoxynucleotide triphosphates) and buffers required for the amplification reaction. Here, the volume of the enzymes, dNTPs (deoxynucleotide triphosphates) and buffers required for the amplification reaction included in the reaction premix is ​​not specifically limited and can be adjusted according to the experiment. The denuclease-free water may be denuclease-free water that has been sterilized.

[0035] In some embodiments, the above-mentioned individual flow channels may include a first preset number of first capillary flow channels 5, a second capillary flow channel 10, a first siphon flow channel 11, a second siphon flow channel 12, a first fluid diversion flow channel 13, a third capillary flow channel 18, a quantitative buffer flow channel 24, a first connecting flow channel and the above-mentioned second preset number of second connecting flow channels. Among them, the above-mentioned second capillary flow channel 10 may be a flow channel for connecting the above-mentioned sample injection chamber 9 with one end of the above-mentioned first mixing chamber 2. The above-mentioned first siphon flow channel 11 may be a flow channel for connecting the other end of the above-mentioned first mixing chamber 2 with one end of the above-mentioned first reaction chamber 1. The above-mentioned second siphon flow channel 12 may be a flow channel for connecting the other end of the above-mentioned second mixing chamber 21 with one end of the above-mentioned first reaction chamber 1. The above-mentioned third capillary flow channel 18 may be a flow channel for connecting the other end of the above-mentioned first liquid buffer chamber 20 with the head end of the above-mentioned first fluid diversion flow channel 13. The quantitative buffer flow channel 24 may be a flow channel for connecting the other end of the first reaction chamber 1 with one end of the first liquid buffer chamber 20. The first connecting flow channel may be a flow channel for connecting the second reagent storage chamber 19 and the second mixing chamber 21. The second preset number of second connecting flow channels may be a flow channel for connecting the second preset number of first liquid quantitative chambers 14 with the first fluid diversion flow channel 13. The first fluid diversion flow channel 13 may be a flow channel for diverting liquid to the second preset number of first liquid quantitative chambers 14. The first capillary flow channel 5 may be a flow channel for connecting the first mixing chamber 2 and the first reagent storage chamber 3. The materials of the first preset number of first capillary channels 5, the second capillary channels 10, the first siphon channels 11, the second siphon channels 12, the first fluid diversion channels 13, the third capillary channels 18, the quantitative buffer channels 24, the first connecting channels and the second preset number of second connecting channels can all be polymethyl methacrylate (PMMA), polycarbonate (PC), polystyrene (PS), polypropylene (PP) and glass. Here, the specific values ​​of the width and depth of the first fluid diversion channels 13, the quantitative buffer channels 24, the first connecting channels and the second preset number of second connecting channels included in the above-mentioned channels are not limited and can be adjusted according to experimental requirements. For example, the width and depth of the first fluid diversion channels 13, the quantitative buffer channels 24, the first connecting channels and the second preset number of second connecting channels included in the above-mentioned channels can all be between 0.1-1 mm. Here, there is no limitation on the width and depth of the first preset number of first capillary channels 5, second capillary channels 10, first siphon channels 11, second siphon channels 12, and third capillary channels 18 included in each of the above-mentioned channels. For example, the width and depth of the first preset number of first capillary channels 5, second capillary channels 10, first siphon channels 11, second siphon channels 12, and third capillary channels 18 included in each of the channels can be between 0.1 and 0.5 mm.Each of the above-mentioned flow channels is used to transport liquid. It should be noted that the width and depth of the above-mentioned quantitative buffer flow channel 24 and the above-mentioned first fluid diversion flow channel 13 can be set to be greater than the width and depth of the above-mentioned first preset number of first capillary flow channels 5, the above-mentioned second capillary flow channels 10, the above-mentioned first siphon flow channel 11, the above-mentioned second siphon flow channel 12, and the above-mentioned third capillary flow channel 18.

[0036] In some embodiments, the sample injection chamber 9 is connected to the first mixing chamber 2 through the second capillary flow channel 10. The first reagent storage chamber 3 is connected to the first mixing chamber 2 through the first preset number of first capillary flow channels 5. One end of the first mixing chamber 2 is connected to the first reaction chamber 1 through the first siphon flow channel 11. One end of the second mixing chamber 21 is connected to the first reaction chamber 1 through the second siphon flow channel 12. The second reagent storage chamber 19 is connected to the second mixing chamber 21 through the first connecting flow channel. The third reagent storage chamber 23 is connected to the second mixing chamber 21 through the target capillary valve. One end of the first reaction chamber 1 is connected to one end of the first liquid buffer chamber 20 through the quantitative buffer flow channel 24. The other end of the first liquid buffer chamber 20 is connected to the head end of the first fluid diversion flow channel 13 through the third capillary flow channel 18. The first fluid diversion channel 13 is provided with the second preset number of liquid diversion ports. The second preset number of liquid diversion ports are connected to one end of the second preset number of first liquid quantitative chambers 14 through the second preset number of second connecting channels. The other end of the second preset number of first liquid quantitative chambers 14 is connected to the second preset number of amplification detection chambers 25 through the second preset number of first capillary valves 15.

[0037] Alternatively, if Figure 1 As shown, at least one first mixing column 4 is disposed in the first reagent storage chamber 3 , and at least one first spoiler column 7 and at least one second spoiler column 8 are disposed in the first reaction chamber 1 .

[0038] Alternatively, if Figure 1As shown, the first mixing chamber 2 is provided with a first spoiler 6, and the second mixing chamber 21 is provided with at least one third spoiler column 22. It should be noted that the at least one first mixing column 4, the at least one first spoiler column 7 and the at least one second spoiler column 8, the first spoiler 6 and the at least one third spoiler column 22 can all improve the mixing efficiency of the solution in the chamber, and can make the mixing of the solution in the chamber more sufficient. The thickness of the first spoiler 6 can be between 3mm-4mm. Here, the thickness of the first spoiler 6 is not specifically limited. It can be adjusted according to experimental requirements. The diameter of the spoiler column in the at least one first spoiler column 7, the at least one second spoiler column 8 and the at least one third spoiler column 22 can be in the range of 3mm-4mm. The diameter and height of the spoiler column in the at least one first spoiler column 7, the at least one second spoiler column 8 and the at least one third spoiler column 22 are not limited here, and can be adjusted according to experimental requirements. The diameter of the first mixing column 4 can be in the range of 3mm-4mm. The diameter and height of the first mixing column 4 are not specifically limited and can be adjusted according to experimental requirements. For example, the height of the spoiler column can be 2 mm, and the height of the mixing column can be 2 mm.

[0039] Alternatively, if Figure 1 As shown, at least two sample loading holes are provided on the sample injection chamber 9, the first reagent storage chamber 3, the second reagent storage chamber 19, and the third reagent storage chamber 23. The at least two sample loading holes may include a sample loading hole for injecting a solution, and a sample loading hole for discharging the gas generated by the reaction in the centrifugal microfluidic chip for gene detection of knee sports injuries. Here, the diameter of each of the at least two sample loading holes is not specifically limited and can be adjusted according to experimental requirements. For example, the diameter of each of the at least two sample loading holes can be 2 mm.

[0040] Alternatively, if Figure 1 As shown, the centrifugal microfluidic chip for genetic detection of sports injuries of knee joints may further include a first waste liquid chamber 17, the first waste liquid chamber 17 is connected to the end of the first fluid diversion channel 13, and the first waste liquid chamber 17 is filled with a water-absorbing material 16. The filled water-absorbing material 16 may be a highly absorbent resin or a hydrogel. The filled water-absorbing material 16 may be used to quickly absorb the waste liquid, thereby preventing the waste liquid from overflowing, reducing the contamination of the internal chamber of the centrifugal microfluidic chip for genetic detection of sports injuries of knee joints, and the leakage of the waste liquid.

[0041] Alternatively, if Figure 1As shown, each of the second preset number of amplification detection chambers 25 includes probes and primers, and the probes and primers included in each of the second preset number of amplification detection chambers 25 are different. The gene loci detected by each of the second preset number of amplification detection chambers 25 are different, and the probes and primers included in the amplification detection chambers 25 are used to detect the gene loci corresponding to the amplification detection chambers 25.

[0042] Alternatively, if Figure 1 As shown, the distance between each adjacent two amplification detection chambers 25 in the above-mentioned second preset number of amplification detection chambers 25 is the same. Among them, the distance between each adjacent two amplification detection chambers 25 in the above-mentioned second preset number of amplification detection chambers 25 can be greater than 2 mm. It should be noted that the number of the above-mentioned second preset number of amplification detection chambers 25 is greater than the number of gene loci to be detected, thereby, the remaining amplification detection chambers 25 can be used as spare amplification detection chambers 25, and more gene loci can be detected at one time, reducing the situation where the number of amplification detection chambers 25 is insufficient. And the interval greater than 2 mm can facilitate the observation of the detection reaction of each amplification detection chamber of the above-mentioned centrifugal microfluidic chip for genetic detection of knee sports injuries.

[0043] Alternatively, if Figure 1 , Figure 2 or Figure 3As shown, the first reaction chamber 1, the first mixing chamber 2, the first reagent storage chamber 3, the sample injection chamber 9, the second preset number of first liquid quantitative chambers 14, the first waste liquid chamber 17, the second reagent storage chamber 19, the first liquid buffer chamber 20, the second mixing chamber 21, the third reagent storage chamber 23 and the second preset number of amplification detection chambers 25 are diffusely distributed at the preset center position 02. The first reagent storage chamber 3, the sample injection chamber 9, the third reagent storage chamber 23 and the second reagent storage chamber 19 are located in the innermost circumferential area of ​​the centrifugal microfluidic chip for knee sports injury gene detection. The first mixing chamber 2 and the second mixing chamber 21 are located in the second circumferential area of ​​the centrifugal microfluidic chip for knee sports injury gene detection. The first reaction chamber 1 and the first liquid buffer chamber 20 are located in the third circumferential area of ​​the centrifugal microfluidic chip for knee sports injury gene detection. The second preset number of first liquid quantitative chambers 14 are located in the fourth circumferential area of ​​the centrifugal microfluidic chip for genetic detection of knee sports injuries. The second preset number of amplification detection chambers 25 are located in the outermost circumferential area of ​​the centrifugal microfluidic chip for genetic detection of knee sports injuries. Among them, the preset center position 02 can be a hollow structure, and the centrifugal microfluidic chip for genetic detection of knee sports injuries can be installed on the carrier 04 through the preset center position 02. In the use state, the centrifugal microfluidic chip for genetic detection of knee sports injuries is installed on the carrier 04. When the carrier 04 is running, the carrier 04 drives the centrifugal microfluidic chip for genetic detection of knee sports injuries to rotate clockwise or counterclockwise with the preset center position 02 as the reference.

[0044] Alternatively, if Figure 2 or Figure 3As shown, the center of the centrifugal microfluidic chip for genetic detection of sports injuries of knee joints is a hollow structure, and the hollow structure is connected to the first fluid diversion flow channel 13 through the main ventilation flow channel, and the other end of the first mixing chamber 2, the other end of the second mixing chamber 21, and the first liquid buffer chamber 20 are connected to the main ventilation flow channel through a sub-ventilation flow channel. Among them, the center of the centrifugal microfluidic chip for genetic detection of sports injuries of knee joints can represent the preset center position 02. The main ventilation flow channel and the sub-ventilation flow channels are mainly used to introduce external gas, control, detect and remove gas in the chamber. The materials of the main ventilation flow channel and the sub-ventilation flow channels can be polydimethylsiloxane (PDMS), polymethyl methacrylate (PMMA) and glass. There is no limitation on the material and size of the main ventilation flow channel and the sub-ventilation flow channels, which can be adjusted according to the experiment. The center of the centrifugal microfluidic chip for genetic detection of sports injuries of knee joints can represent the preset center position 02. The above-mentioned carrier 04 is a machine that can be used to fix the above-mentioned centrifugal microfluidic chip for knee sports injury gene detection, and drive the above-mentioned centrifugal microfluidic chip for knee sports injury gene detection to rotate clockwise or counterclockwise. The above-mentioned carrier 04 can be set with a temperature control cycle amplification program. The above-mentioned carrier 04 may include a tray 03 and a motor. The speed and direction of the above-mentioned carrier 04 are both adjustable. The above-mentioned tray 03 can heat the above-mentioned centrifugal microfluidic chip for knee sports injury gene detection through the set temperature control cycle amplification program to achieve multi-cycle amplification processing. The motor of the above-mentioned carrier 04 can be set at the axis position of the above-mentioned carrier 04. In the use state, the solution in the above-mentioned centrifugal microfluidic chip for knee sports injury gene detection can be mixed, the solution can be pushed into each chamber, and the above-mentioned centrifugal microfluidic chip for knee sports injury gene detection can be heated to achieve multi-cycle amplification processing.

[0045] Optionally, the operation method of the centrifugal microfluidic chip for gene detection of knee sports injuries is as follows:

[0046] The first step is to capture the image information of the centrifugal microfluidic chip. The image information can represent the image of the centrifugal microfluidic chip. In practice, a high-precision camera can be used to capture the image information of the centrifugal microfluidic chip.

[0047] The second step is to upload the image information to a crack detection processor to obtain crack detection result information corresponding to the centrifugal microfluidic chip. The crack detection processor may be a processor for detecting whether there are cracks in the centrifugal microfluidic chip through an image of the centrifugal microfluidic chip. The crack detection result information may indicate whether there are cracks in the centrifugal microfluidic chip or not.

[0048] In the third step, in response to determining that the crack detection result information meets the preset qualified condition, the centrifugal microfluidic chip is placed on the carrier 04. A motor for rotating the centrifugal microfluidic chip is provided on the carrier 04. The preset qualified condition may be that the crack detection result information indicates that the centrifugal microfluidic chip has no cracks.

[0049] In the process of adopting technical solutions to solve the above-mentioned technical problem one, the following technical problem two is often accompanied: when using the conventional sample and detection reagent injection method (mixing the sample and the detection reagent and injecting them into each amplification detection chamber in the microfluidic chip) to inject the sample and detection reagent into each amplification detection chamber of the microfluidic chip, it is necessary to mix multiple types of reagents and achieve uniform mixing, resulting in high technical requirements for detection personnel, more detection steps, and a long time for manual mixing. In addition, the number of amplification detection chambers is large, and they need to be injected one by one, which takes a long time, resulting in greater difficulty in detection and a longer detection time. For the above-mentioned technical problem two, the conventional solution is generally: use a machine to mix the sample and part of the reagents. The inventors took into account the shortcomings of using a machine to mix the sample and part of the reagents, and combined with the inventors' advantages in microfluidic chips and sample processing, we decided to adopt the following solution:

[0050] The fourth step is to inject the sample solution of the first preset volume into the sample injection chamber 9 of the above-mentioned centrifugal microfluidic chip. The above-mentioned first preset volume is 200 microliters. The above-mentioned sample solution can be a blood sample, a cell suspension or other biological fluids. It should be noted that the volume of the sample solution injected into each amplification detection chamber 25 of the above-mentioned centrifugal microfluidic chip can be at least 3 microliters, and the number of amplification detection chambers 25 of the above-mentioned centrifugal microfluidic chip can be 63, so the volume of the sample solution injected into the above-mentioned centrifugal microfluidic chip can be at least 200 microliters. Here, the specific volume value of the sample solution injected into the above-mentioned centrifugal microfluidic chip is not limited and can be adjusted according to the experiment. In practice, the sample solution of the first preset volume can be injected into the above-mentioned centrifugal microfluidic chip through the sample injection port set on the above-mentioned sample injection chamber 9.

[0051] The fifth step is to inject the second preset volume of the lysis reagent into the first reagent storage chamber 3 of the centrifugal microfluidic chip. The second preset volume is 2000 microliters. The ratio between the volume of the sample solution and the volume of the lysis reagent can be 1:5 or 1:10. In practice, the lysis reagent can be injected through the injection hole provided on the first reagent storage chamber 3. It should be noted that the added lysis reagent can be used to lyse the cells in the sample solution to release DNA. Here, the volume of the lysis reagent is not specifically limited and can be adjusted according to experimental requirements.

[0052] The sixth step is to inject the third preset volume of denucleated water into the second reagent storage chamber 19 of the centrifugal microfluidic chip, wherein the third preset volume is 450 microliters. The ratio between the volume of the sample solution and the volume of the denucleated water can be 1:2.25. In practice, the denucleated water can be injected into the centrifugal microfluidic chip through the sample loading hole provided on the second reagent storage chamber 19. It should be noted that the injected denucleated water can be used to clean samples and dilute lysate, thereby improving the accuracy of subsequent detection. Here, the volume of the denucleated water is not specifically limited and can be adjusted according to experimental requirements.

[0053] The seventh step is to inject the fourth preset volume of the reaction premix into the third reagent storage chamber 23 of the centrifugal microfluidic chip, wherein the fourth preset volume is 750 microliters. The ratio between the volume of the sample solution and the volume of the reaction premix can be 1:3.75. In practice, the reaction premix can be injected into the microfluidic chip through the loading hole provided on the third reagent storage chamber 23. It should be noted that the injected reaction premix can enable the amplification reaction to proceed efficiently. Here, the volume of the reaction premix is ​​not specifically limited and can be adjusted according to experimental requirements.

[0054] In the eighth step, according to the first speed and the first direction of the motor, the centrifugal microfluidic chip is rotated so that the first preset volume of the sample solution in the centrifugal microfluidic chip flows into the channel of the first mixing chamber 2, the lysis reagent in the first reagent storage chamber 3 flows into the first mixing chamber 2 through the first capillary flow channel 5, and the denucleic acid water in the second reagent storage chamber 19 and the reaction premix in the third reagent storage chamber 23 flow into the second mixing chamber 21. The first speed is set to 1000RPM, and the first direction is set to clockwise rotation. It should be noted that the speed of the motor needs to be set to a low speed so that the first preset volume of the sample solution in the centrifugal microfluidic chip flows into the channel of the first mixing chamber 2, the lysis reagent in the first reagent storage chamber 3 flows into the first mixing chamber 2 through the first capillary flow channel 5, and the denucleic acid water in the second reagent storage chamber 19 and the reaction premix in the third reagent storage chamber 23 flow into the second mixing chamber 21. Therefore, the speed setting range of the motor can be 500-1500RPM. In practice, the first speed of the motor can be set to 1000RPM, the direction can be set to clockwise rotation, and the rotation time can be set to 10 seconds, so that the first preset volume of sample solution in the centrifugal microfluidic chip flows into the channel of the first mixing chamber 2, the lysis reagent in the first reagent storage chamber 3 flows into the first mixing chamber 2 through the first capillary channel 5, and the denucleic acid water in the second reagent storage chamber 19 and the reaction premix in the third reagent storage chamber 23 flow into the second mixing chamber 21.

[0055] In the ninth step, the centrifugal microfluidic chip is rotated according to the first preset counterclockwise speed and the first preset clockwise speed within the first preset time to mix the sample solution in the first mixing chamber 2 and the lysis reagent to obtain a first mixed solution, and the denucleic acid water and the reaction premix in the second mixing chamber 21 are uniformly mixed to obtain a second mixed solution. Among them, the first preset time can be 5 minutes. The first preset counterclockwise speed can be 100RPM. The first preset clockwise speed can be 100RPM. It should be noted that within the first preset time, the method for switching the rotation direction of the motor is: the motor switches to counterclockwise rotation after 0.2 seconds of clockwise rotation, and the motor switches to clockwise rotation after 0.2 seconds of counterclockwise rotation. Setting the rotation time to 5 minutes, the setting of switching the rotation direction of the motor and the setting of the speed can fully mix the sample solution and the lysis reagent in the first mixing chamber 2. When the rotation speed of the motor is set between 100RPM-200RPM, the sample solution in the first mixing chamber 2 and the lysing reagent have a better mixing effect. The acceleration of the motor is set to 5000RPM / s. When the acceleration is 5000RPM / s, the rotation effect of the carrier 04 is relatively stable. Here, there is no limitation on the first preset time and the rotation speed and direction of the motor. The rotation of the motor can fully mix the sample solution in the first mixing chamber 2 and the lysing reagent.

[0056] The tenth step is to rotate the centrifugal microfluidic chip according to the second preset counterclockwise speed, so that the first mixed liquid in the first mixing chamber 2 flows into the first reaction chamber 1 through the first siphon channel 11. The second preset counterclockwise speed may be 1000RPM. In practice, the speed of the motor is switched to 1000RPM, and the direction of the motor is set to counterclockwise rotation, so that the first mixed liquid in the first mixing chamber 2 flows into the first reaction chamber 1 through the first siphon channel 11. It should be noted that only when the speed of the motor is set to be greater than or equal to 1000RPM, the first mixed liquid in the first mixing chamber 2 can flow into the first reaction chamber 1 through the first siphon channel 11 under the combined action of Euler force and centrifugal force.

[0057] In the eleventh step, the centrifugal microfluidic chip is rotated according to the second preset clockwise speed to allow the second mixed liquid in the second mixing chamber 21 to flow into the first reaction chamber 1 through the second siphon channel 12. The second preset clockwise speed may be 1000RPM. In practice, the direction of the motor may be switched to clockwise to allow the second mixed liquid in the second mixing chamber 21 to flow into the first reaction chamber 1 through the second siphon channel 12. It should be noted that only when the speed of the motor is set to be greater than or equal to 1000RPM can the second mixed liquid in the second mixing chamber 21 flow into the first reaction chamber 1 through the second siphon channel 12 under the combined action of Euler force and centrifugal force.

[0058] In the twelfth step, the centrifugal microfluidic chip is rotated according to the first preset counterclockwise speed and the first preset clockwise speed within the second preset time, so as to uniformly mix the first mixed solution and the second mixed solution in the first reaction chamber 1 to obtain the target premixed solution. Among them, the second preset time can be 1 minute. In practice, the motor can be switched from the second preset clockwise speed to the first preset counterclockwise speed, and then the first preset counterclockwise speed and the first preset clockwise speed are alternately switched every two seconds within the second preset time. The acceleration during the switching process can be 5000RPM / s. The carrier 04 can be rotated more stably. It should be noted that setting the rotation time to 1 minute and switching between the first preset counterclockwise speed and the first preset clockwise speed can be used to uniformly mix the first mixed solution and the second mixed solution in the first reaction chamber 1. Here, the speed of the motor is not limited, and the first mixed solution and the second mixed solution in the first reaction chamber 1 can be uniformly mixed.

[0059] In step 13, the centrifugal microfluidic chip is rotated according to the second preset clockwise rotation speed, so that the target premixed liquid in the first reaction chamber 1 flows into the second preset number of first liquid quantitative chambers 14. It should be noted that when the speed of the motor is set to be greater than or equal to 1000 RPM and the direction is set to rotate clockwise, the target premixed liquid in the first reaction chamber 1 can flow into the second preset number of first liquid quantitative chambers 14 through the first fluid diversion channel 13 under the combined action of Euler force and centrifugal force.

[0060] In the fourteenth step, the centrifugal microfluidic chip is rotated according to the third preset clockwise rotation speed, so that the target premixed liquid in the second preset number of first liquid quantitative chambers 14 enters the second preset number of amplification detection chambers 25 through the second preset number of capillary valves in sequence. Among them, the third preset clockwise rotation speed can be 3000RPM. It should be noted that when the speed of the motor is set to be greater than or equal to 3000RPM, and the direction is set to clockwise rotation, the target premixed liquid in the second preset number of first liquid quantitative chambers 14 can enter the second preset number of amplification detection chambers 25 through the second preset number of capillary valves under the combined action of Euler force and centrifugal force.

[0061] In the fifteenth step, the temperature-controlled cycle amplification program on the carrier 04 is started to perform multi-cycle amplification on the centrifugal microfluidic chip. In practice, 45 cycles can be performed to perform multi-cycle amplification on the centrifugal microfluidic chip. Each cycle is as follows: first, the temperature of the carrier 04 is set to 95°C to heat the centrifugal microfluidic chip for 30 seconds for denaturation treatment, then, it is switched to 55°C to heat the centrifugal microfluidic chip for 30 seconds for annealing treatment, and then it is switched to 72°C to heat the centrifugal microfluidic chip for 30 seconds for extension treatment. The time taken to perform multi-cycle amplification on the centrifugal microfluidic chip is approximately between 1 hour and 1.5 hours. It should be noted that the setting of the temperature, the setting of the heating time and the time-consuming setting of the multi-cycle amplification process can be used to achieve efficient amplification of the target gene fragment by the centrifugal microfluidic chip to achieve gene detection.

[0062] The above technical solution and its related contents, as an inventive point of the embodiment of the present disclosure, solve the technical problem 2 mentioned in the background technology, "the difficulty of detection is relatively large and the detection time is long". The factors that lead to the difficulty of detection and the long detection time are often as follows: when the conventional sample and detection reagent injection method (mixing the sample and the detection reagent and injecting them into each amplification detection chamber in the microfluidic chip) is used to inject the sample and the detection reagent into each amplification detection chamber of the microfluidic chip, it is necessary to mix multiple types of reagents and achieve uniform mixing, resulting in high technical requirements for the detection personnel, more detection steps, and a long time for manual uniform mixing, and the number of amplification detection chambers is large, and injection is required one by one. If the above factors are solved, the effect of reducing the difficulty of detection and shortening the time of detection can be achieved. In order to achieve this effect, the present disclosure only needs to inject the sample and the detection reagent into the above-mentioned centrifugal microfluidic chip, and then by setting the rotation speed and direction of the carrier 04, the sample and the reagent can be mixed, and the mixed solution can be flowed to each amplification detection chamber, thereby reducing the difficulty of detection and shortening the detection time.

[0063] In the process of adopting technical solutions to solve the above-mentioned technical problem one, the following technical problem three is often accompanied: when using a centrifugal microfluidic chip, the sample or reagent is directly injected into the centrifugal microfluidic chip without checking the centrifugal microfluidic chip. The presence of cracks in the centrifugal microfluidic chip causes leakage of reagents and samples, resulting in low accuracy of the test results and waste of reagents and samples. For the above-mentioned technical problem three, the conventional solution is generally: before using the centrifugal microfluidic chip, use a microscope or the naked eye to check whether the centrifugal microfluidic chip has damage or cracks. The inventor took into account the shortcomings of using a microscope or the naked eye to detect whether the centrifugal microfluidic chip has damage or cracks, and the inventor's unit has experience in detecting whether the centrifugal microfluidic chip has cracks. In combination with the situation of the cooperative unit commissioned for development, we decided to adopt the following solution:

[0064] Optionally, the crack detection processor is further configured to:

[0065] The first step is to receive the image information of the centrifugal microfluidic chip.

[0066] The second step is to generate image feature information corresponding to the image information according to the image information. The image feature information may represent the prior features of the image information. The image feature information may represent the feature vector, texture feature, shape feature or color feature of the image. The feature vector may represent the local features of the image information generated by the feature points of the image information. For example, the feature vector may represent the scale and gradient direction of the image information. In practice, first, the crack detection processor may detect the feature points of the image information by a feature point detection algorithm. For example, the feature point detection algorithm may be a SIFT algorithm. Then, the feature vector corresponding to the image information may be obtained according to the feature points by a sparse coding method. After that, the texture features of the image information may be extracted by a texture feature extraction algorithm to obtain the texture features corresponding to the image information. For example, the texture feature extraction algorithm may be a Fourier transform algorithm. Secondly, the shape features of the image information may be extracted by an edge detection algorithm. For example, the edge detection algorithm may be a Canny edge detection. After that, the color features corresponding to the image information may be obtained by a principal component analysis method (PCA). Finally, the obtained feature vector, texture feature, shape feature and color feature are determined as image feature information corresponding to the above image information.

[0067] The third step is to generate image guidance feature information corresponding to the above image information based on the above image information. The above image guidance feature information can represent the histogram corresponding to the above image information after equalization processing. In practice, the above crack detection processor can generate a histogram corresponding to the above image information through the cv2.calcHist function in OpenCV. Then, the above histogram is equalized according to the histogram equalization algorithm to obtain the histogram after equalization processing as the image guidance feature information of the above image information.

[0068] The fourth step is to perform brightness enhancement processing on the image feature information to obtain the image feature information after brightness enhancement processing. In practice, the crack detection processor can perform brightness enhancement processing on the image feature information by using a Retinex algorithm to obtain the image feature information after brightness enhancement processing.

[0069] The fifth step is to fuse the image feature information after the brightness enhancement processing with the image information to obtain the first fused image information. The first fused image information can represent a feature map that fuses the first image feature and the second image feature. The first image feature can represent the image feature of the image feature information after the brightness enhancement processing. The second image feature can represent the image feature of the image information. In practice, the crack detection processor can perform element-by-element multiplication processing on the image feature information after the brightness enhancement processing and the image information to perform fusion processing to obtain the first fused image information.

[0070] In the sixth step, the first fused image information is fused with the image feature information to obtain the second fused image information. The second fused image information may represent a feature map fused with the second image feature and the third image feature. The third image feature may represent the image feature of the first fused image information. In practice, the crack detection processor may perform element-by-element addition processing on the first fused image information and the image feature information to perform fusion processing to obtain the second fused image information.

[0071] In the seventh step, the second fused image information is subjected to pixel update processing to obtain updated second fused image information. In practice, the crack detection processor may perform pixel reorganization downsampling processing on the second fused image information through each pixel reorganization downsampling module to update the second fused image information to obtain updated second fused image information. Among them, each pixel reorganization downsampling module may represent each pixel reorganization downsampling module with different corresponding step sizes. Each pixel reorganization downsampling module may implement a dimensionality reduction operation on the second fused image information.

[0072] In the eighth step, the second fused image information after the update is subjected to image compensation processing, and the second fused image information after the image compensation processing is obtained as the first denoised image information. In practice, the crack detection processor can perform image compensation processing on the second fused image information after the update through a multi-class replacement and refinement module to obtain the first denoised image information. The multi-class replacement and refinement module can be used to compensate for the image information lost in the second fused image information after the update due to denoising.

[0073] The ninth step is to fuse the first denoised image information and the image guidance feature information to obtain the target image feature information after the fusion process. The target image feature information can represent a feature map including a fourth image feature and a fifth image feature. The fourth image feature can represent the image feature of the first denoised image information. The fifth image feature can represent the image feature of the image guidance feature information. In practice, the crack detection processor can perform element-by-element multiplication of the first denoised image information and the image guidance feature information to perform fusion process to obtain the target image feature information after the fusion process.

[0074] In the tenth step, the target image feature information is normalized to obtain the normalized target image feature information. In practice, the crack detection processor may normalize the target image feature information through a normalization layer. For example, the normalization layer may be LN (Layer Normalization).

[0075] The eleventh step is to generate a channel attention weight map and a spatial attention weight map corresponding to the target image feature information according to the target image feature information. The channel attention weight map can characterize the weight vector of each channel in the target image feature information, and characterize the importance of each channel in the task. The spatial attention weight map can characterize the weight matrix with the same spatial size as the target image feature information, and characterize the importance of each spatial position in the target image feature information in the task. In practice, the crack detection processor can input the target image feature information into the channel attention module to obtain the channel attention weight map, and input the target image feature information into the spatial attention module to obtain the spatial attention weight map. The channel attention module can be used to generate the weight value of each channel in the target image feature information. The spatial attention module can be used to generate a spatial attention weight map corresponding to the target image feature information.

[0076] In the twelfth step, the channel attention weight map and the spatial attention weight map are fused to obtain a fused feature map. The fused feature map may represent a feature map obtained by element-by-element addition of the channel attention weight map and the spatial attention weight map. In practice, the crack detection processor may perform element-by-element addition of the channel attention weight map and the spatial attention weight map to obtain a fused feature map.

[0077] In the thirteenth step, the fused feature map is normalized to obtain a normalized fused feature map. In practice, the crack detection processor may normalize the fused feature map through a normalization layer. For example, the normalization layer may be LN (Layer Normalization).

[0078] In step 14, the fused feature map after normalization is denoised to obtain second denoised image information as target image information corresponding to the image information. In practice, the crack detection processor can denoise the fused feature map after normalization through a feedforward neural network to obtain second denoised image information as target image information corresponding to the image information.

[0079] In the fifteenth step, the target image information is input into the crack detection model to obtain crack detection result information. The crack detection model may be a model that takes the target image information corresponding to the centrifugal microfluidic chip as input and the crack detection result information corresponding to the centrifugal microfluidic chip as output. The crack detection model may be a target detection model. It should be noted that the training data for training the crack detection model may be a set of crack image information of each centrifugal microfluidic chip containing cracks, and each crack image information in the crack image information set includes a crack annotation. Each crack image information in the crack image information set is processed by the methods of the second to fourteenth steps, which is the same as the processing method of the target image information. The training method of the crack detection model may be a batch training method. For example, the crack detection model may be a YOLO model.

[0080] The above technical solution and its related contents, as an inventive point of the embodiment of the present disclosure, solve the technical problem three mentioned in the background technology, "causing low accuracy of the test results and waste of reagents and samples". The factors that lead to low accuracy of the test results and waste of reagents and samples are often as follows: when using a centrifugal microfluidic chip, the sample or reagent is directly injected into the centrifugal microfluidic chip without checking the centrifugal microfluidic chip, and the centrifugal microfluidic chip has cracks that cause leakage of reagents and samples. If the above factors are solved, the effect of reducing the low accuracy of the test results and reducing the waste of reagents and samples can be achieved. In order to achieve this effect, the present disclosure takes an image of the centrifugal microfluidic chip before using the centrifugal microfluidic chip, and then uploads the image to the above crack detection processor. Since the volume of the centrifugal microfluidic chip is small, an image with high image quality is required to detect whether there is a crack. Therefore, the image is denoised and enhanced by the above crack detection processor to obtain image information with high image quality, and then the above crack detection model is used to detect whether there is a crack in the image information to achieve crack detection of the centrifugal microfluidic chip. Because the crack detection is performed based on the image of the centrifugal microfluidic chip after denoising and image enhancement before using the centrifugal microfluidic chip, the accuracy of crack detection can be improved, and the centrifuge microfluidic chip can be further used when there are no cracks in the centrifugal microfluidic chip, thereby reducing the low accuracy of the detection results and reducing the waste of reagents and samples.

[0081] The above-mentioned various embodiments of the present disclosure have the following beneficial effects: through a centrifugal microfluidic chip for knee sports injury gene detection according to some embodiments of the present disclosure, the difficulty of detection can be reduced, the time consumption of detection can be shortened, and the situation of consuming a large number of knee sports injury gene detection kits can be reduced. The reasons for the difficulty of detection, the long detection time, and the large number of knee sports injury gene detection kits are: when using polymerase chain reaction technology for detection, the polymerase chain reaction technology has high requirements for instruments and equipment and the detection skills of detection personnel, and requires cumbersome detection steps, resulting in greater difficulty in detection and longer detection time; when using a knee sports injury gene detection kit for detection, each knee sports injury gene detection kit detects fewer gene sites, and the number of susceptible gene sites of knee sports injury genes is large, resulting in more times of detection, more knee sports injury gene detection kits used, resulting in longer detection time and more consumption of knee sports injury gene detection kits.Based on this, some embodiments of the present disclosure are centrifugal microfluidic chips for genetic detection of knee sports injuries, characterized in that the centrifugal microfluidic chip includes a plastic substrate, various flow channels, a target capillary valve and a second preset number of first capillary valves, wherein the plastic substrate includes a first reaction chamber, a first mixing chamber, a first reagent storage chamber, a sample injection chamber, a second preset number of first liquid quantitative chambers, a second reagent storage chamber, a first liquid buffer chamber, a second mixing chamber, a third reagent storage chamber and a second preset number of amplification detection chambers; the various flow channels include a first preset number of first capillary flow channels, a second capillary flow channel, a first siphon flow channel, a second siphon flow channel, a first fluid diversion flow channel, a third capillary flow channel, a quantitative buffer flow channel, a first connecting flow channel and the second preset number of second connecting flow channels; the sample injection chamber is connected to the first mixing chamber via the second capillary flow channel, the first reagent storage chamber is connected to the first mixing chamber via the first preset number of first capillary flow channels, and the first mixing chamber One end of the first reaction chamber is connected to the first reaction chamber through the first siphon flow channel, one end of the second mixing chamber is connected to the first reaction chamber through the second siphon flow channel, the second reagent storage chamber is connected to the second mixing chamber through the first connecting flow channel, the third reagent storage chamber is connected to the second mixing chamber through the target capillary valve, one end of the first reaction chamber is connected to one end of the first liquid buffer chamber through the quantitative buffer flow channel, the other end of the first liquid buffer chamber is connected to the head end of the first fluid diversion flow channel through the third capillary flow channel, the first fluid diversion flow channel is provided with the second preset number of liquid diversion ports, the second preset number of liquid diversion ports are connected to one end of the second preset number of first liquid quantitative chambers through the second preset number of second connecting flow channels, and the other end of the second preset number of first liquid quantitative chambers is connected to the second preset number of amplification detection chambers through the second preset number of first capillary valves. Also, only the centrifugal microfluidic chip for knee sports injury gene detection can be used to detect whether there are susceptibility gene sites related to knee sports injury genes in the sample, without the need for higher instruments and equipment for detection. Therefore, the difficulty of sample detection can be reduced. Also, only the sample and the detection reagent need to be injected into the centrifugal microfluidic chip for knee sports injury gene detection to automatically detect the sample and the reagent, thereby lowering the requirements for the detection skills of the detection personnel. Also, because the centrifugal microfluidic chip contains the second preset number of amplification detection chambers, the second number can be 63, thereby, multiple existing knee sports injury gene sites can be detected simultaneously, rather than multiple detections by using multiple knee sports injury gene detection kits.Therefore, the centrifugal microfluidic chip for genetic testing of knee sports injuries can shorten the steps of genetic testing, shorten the time of genetic testing, and eliminate the need to consume a large number of genetic testing kits for knee sports injuries, thereby saving testing resources.

[0082] Reference below Figure 4 , which shows a schematic structural diagram of an electronic device 400 (eg, a computing device) suitable for implementing some embodiments of the present disclosure. Figure 4 The electronic device shown is only an example and should not bring any limitation to the functions and scope of use of the embodiments of the present disclosure.

[0083] like Figure 4 As shown, the electronic device 400 may include a processing device 401 (e.g., a central processing unit, a graphics processing unit, etc.), which can perform various appropriate actions and processes according to a program stored in a read-only memory (ROM) 402 or a program loaded from a storage device 408 into a random access memory (RAM) 403. In the RAM 403, various programs and data required for the operation of the electronic device 400 are also stored. The processing device 401, the ROM 402, and the RAM 403 are connected to each other via a bus 404. An input / output (I / O) interface 405 is also connected to the bus 404.

[0084] Typically, the following devices may be connected to the I / O interface 405: an input device 406 including, for example, a touch screen, a touch pad, a keyboard, a mouse, a camera, a microphone, an accelerometer, a gyroscope, etc.; an output device 407 including, for example, a liquid crystal display (LCD), a speaker, a vibrator, etc.; a storage device 408 including, for example, a magnetic tape, a hard disk, etc.; and a communication device 409. The communication device 409 may allow the electronic device 400 to communicate with other devices wirelessly or by wire to exchange data. Although Figure 4 The electronic device 400 is shown with various devices, but it should be understood that it is not required to implement or possess all the devices shown. More or fewer devices may be implemented or possessed instead. Figure 4 Each block shown in the figure may represent one device, or may represent multiple devices as required.

[0085] In particular, according to some embodiments of the present disclosure, the process described above with reference to the flowchart can be implemented as a computer software program. For example, some embodiments of the present disclosure include a computer program product, which includes a computer program carried on a computer-readable medium, and the computer program includes a program code for executing the method shown in the flowchart. In some such embodiments, the computer program can be downloaded and installed from the network through the communication device 409, or installed from the storage device 408, or installed from the ROM 402. When the computer program is executed by the processing device 401, the above-mentioned functions defined in the method of some embodiments of the present disclosure are executed.

[0086] It should be noted that the computer-readable medium recorded in some embodiments of the present disclosure may be a computer-readable signal medium or a computer-readable storage medium or any combination of the above two. The computer-readable storage medium may be, for example, but not limited to, an electrical, magnetic, optical, electromagnetic, infrared, or semiconductor system, device or device, or any combination of the above. More specific examples of computer-readable storage media may include, but are not limited to: an electrical connection with one or more wires, a portable computer disk, a hard disk, a random access memory (RAM), a read-only memory (ROM), an erasable programmable read-only memory (EPROM or flash memory), an optical fiber, a portable compact disk read-only memory (CD-ROM), an optical storage device, a magnetic storage device, or any suitable combination of the above. In some embodiments of the present disclosure, a computer-readable storage medium may be any tangible medium containing or storing a program that can be used by or in combination with an instruction execution system, device or device. In some embodiments of the present disclosure, a computer-readable signal medium may include a data signal propagated in a baseband or as part of a carrier wave, which carries a computer-readable program code. This propagated data signal may take a variety of forms, including but not limited to electromagnetic signals, optical signals, or any suitable combination of the above. The computer readable signal medium may also be any computer readable medium other than a computer readable storage medium, which may send, propagate or transmit a program for use by or in conjunction with an instruction execution system, apparatus or device. The program code contained on the computer readable medium may be transmitted using any suitable medium, including but not limited to: wires, optical cables, RF (radio frequency), etc., or any suitable combination of the above.

[0087] In some embodiments, the client and the server may communicate using any currently known or future developed network protocol such as HTTP (HyperText Transfer Protocol), and may be interconnected with any form or medium of digital data communication (e.g., a communication network). Examples of communication networks include a local area network ("LAN"), a wide area network ("WAN"), an internet (e.g., the Internet), and a peer-to-peer network (e.g., an ad hoc peer-to-peer network), as well as any currently known or future developed network.

[0088] The above-mentioned computer-readable medium may be included in the above-mentioned electronic device; or it may exist independently without being assembled into the electronic device. The above-mentioned computer-readable medium carries one or more programs. When the above-mentioned one or more programs are executed by the electronic device, the electronic device: receives the image information of the above-mentioned centrifugal microfluidic chip. Based on the above-mentioned image information, generate image feature information corresponding to the above-mentioned image information. Based on the above-mentioned image information, generate image guidance feature information corresponding to the above-mentioned image information. Perform brightness enhancement processing on the above-mentioned image feature information to obtain image feature information after brightness enhancement processing. Perform fusion processing on the image feature information after brightness enhancement processing with the above-mentioned image information to obtain first fused image information; perform fusion processing on the above-mentioned first fused image information with the above-mentioned image feature information to obtain second fused image information; perform pixel update processing on the above-mentioned second fused image information to obtain updated second fused image information; perform image compensation processing on the above-mentioned updated second fused image information to obtain the second fused image information after image compensation processing as the first denoised image information; perform fusion processing on the above-mentioned first denoised image information with the above-mentioned image guidance feature information to obtain fused image information. target image feature information; normalizing the target image feature information to obtain the normalized target image feature information; generating a channel attention weight map and a spatial attention weight map corresponding to the target image feature information according to the target image feature information; fusing the channel attention weight map and the spatial attention weight map to obtain a fused feature map; normalizing the fused feature map to obtain a normalized fused feature map; denoising the normalized fused feature map to obtain a second denoised image information as the target image information corresponding to the image information; inputting the target image information into a crack detection model to obtain crack detection result information.

[0089] Computer program code for performing the operations of some embodiments of the present disclosure may be written in one or more programming languages ​​or a combination thereof, including object-oriented programming languages ​​such as Java, Smalltalk, C++, and conventional procedural programming languages ​​such as "C" or similar programming languages. The program code may be executed entirely on the user's computer, partially on the user's computer, as a separate software package, partially on the user's computer and partially on a remote computer, or entirely on a remote computer or server. In cases involving a remote computer, the remote computer may be connected to the user's computer via any type of network, including a local area network (LAN) or a wide area network (WAN), or may be connected to an external computer (e.g., via the Internet using an Internet service provider).

[0090] The flow chart and block diagram in the accompanying drawings illustrate the possible architecture, function and operation of the system, method and computer program product according to various embodiments of the present disclosure. In this regard, each square box in the flow chart or block diagram can represent a module, a program segment or a part of a code, and the module, the program segment or a part of the code contains one or more executable instructions for realizing the specified logical function. It should also be noted that in some implementations as replacements, the functions marked in the square box can also occur in a sequence different from that marked in the accompanying drawings. For example, two square boxes represented in succession can actually be executed substantially in parallel, and they can sometimes be executed in the opposite order, depending on the functions involved. It should also be noted that each square box in the block diagram and / or flow chart, and the combination of the square boxes in the block diagram and / or flow chart can be implemented with a dedicated hardware-based system that performs a specified function or operation, or can be implemented with a combination of dedicated hardware and computer instructions.

[0091] The functions described above herein may be performed at least in part by one or more hardware logic components. For example, without limitation, exemplary types of hardware logic components that may be used include: field programmable gate arrays (FPGAs), application specific integrated circuits (ASICs), application specific standard products (ASSPs), systems on chips (SOCs), complex programmable logic devices (CPLDs), and the like.

[0092] The above descriptions are only some preferred embodiments of the present disclosure and an explanation of the technical principles used. Those skilled in the art should understand that the scope of the invention involved in the embodiments of the present disclosure is not limited to the technical solutions formed by a specific combination of the above-mentioned technical features, but should also cover other technical solutions formed by any combination of the above-mentioned technical features or their equivalent features without departing from the above-mentioned inventive concept. For example, the above-mentioned features are replaced with the technical features with similar functions disclosed in the embodiments of the present disclosure (but not limited to) and the technical solutions formed.

Claims

1. A centrifugal microfluidic chip for gene detection of knee sports injuries, characterized in that: The centrifugal microfluidic chip comprises a plastic substrate, various flow channels, a target capillary valve and a second preset number of first capillary valves, wherein: The plastic substrate comprises a first reaction chamber, a first mixing chamber, a first reagent storage chamber, a sample injection chamber, a second preset number of first liquid quantitative chambers, a second reagent storage chamber, a first liquid buffer chamber, a second mixing chamber, a third reagent storage chamber, and a second preset number of amplification detection chambers; The individual flow channels include a first preset number of first capillary flow channels, a second capillary flow channel, a first siphon flow channel, a second siphon flow channel, a first fluid diversion flow channel, a third capillary flow channel, a quantitative buffer flow channel, a first connecting flow channel, and a second preset number of second connecting flow channels; The sample injection chamber is connected to the first mixing chamber through the second capillary flow channel, the first reagent storage chamber is connected to the first mixing chamber through the first preset number of first capillary flow channels, one end of the first mixing chamber is connected to the first reaction chamber through the first siphon flow channel, one end of the second mixing chamber is connected to the first reaction chamber through the second siphon flow channel, the second reagent storage chamber is connected to the second mixing chamber through the first connecting flow channel, the third reagent storage chamber is connected to the second mixing chamber through the target capillary valve, one end of the first reaction chamber is connected to one end of the first liquid buffer chamber through the quantitative buffer flow channel, the other end of the first liquid buffer chamber is connected to the head end of the first fluid diversion flow channel through the third capillary flow channel, the first fluid diversion flow channel is provided with the second preset number of liquid diversion ports, the second preset number of liquid diversion ports are connected to one end of the second preset number of first liquid quantitative chambers through the second preset number of second connecting flow channels, and the other end of the second preset number of first liquid quantitative chambers is connected to the second preset number of amplification detection chambers through the second preset number of first capillary valves.

2. The centrifugal microfluidic chip for gene detection of knee sports injuries according to claim 1, characterized in that: At least one first mixing column is disposed in the first reagent storage chamber, and at least one first spoiler column and at least one second spoiler column are disposed in the first reaction chamber.

3. The centrifugal microfluidic chip for gene detection of knee sports injuries according to claim 1, characterized in that: A first spoiler is disposed in the first mixing chamber, and at least one third spoiler column is disposed in the second mixing chamber.

4. The centrifugal microfluidic chip for gene detection of knee sports injuries according to claim 1, characterized in that: The sample injection chamber, the first reagent storage chamber, the second reagent storage chamber, and the third reagent storage chamber are each provided with at least two sample addition holes.

5. The centrifugal microfluidic chip for gene detection of knee sports injuries according to claim 1, characterized in that: The centrifugal microfluidic chip for genetic detection of knee sports injuries also includes a first waste liquid chamber, which is connected to the end of the first fluid diversion channel, and the first waste liquid chamber is filled with a water-absorbing material.

6. The centrifugal microfluidic chip for gene detection of knee sports injuries according to claim 1, characterized in that: Each amplification detection chamber in the second preset number of amplification detection chambers includes a probe and a primer, and the probe and primer included in each amplification detection chamber in the second preset number of amplification detection chambers are different.

7. The centrifugal microfluidic chip for gene detection of knee sports injuries according to claim 1, characterized in that: The distance between each two adjacent amplification detection chambers in the second preset number of amplification detection chambers is the same.

8. The centrifugal microfluidic chip for gene detection of knee sports injuries according to claim 5, characterized in that: The first reaction chamber, the first mixing chamber, the first reagent storage chamber, the sample injection chamber, the second preset number of first liquid quantitative chambers, the first waste liquid chamber, the second reagent storage chamber, the first liquid buffer chamber, the second mixing chamber, the third reagent storage chamber and the second preset number of amplification detection chambers are diffusely distributed at a preset circle center position, the first reagent storage chamber, the sample injection chamber, the third reagent storage chamber and the second reagent storage chamber are located in the innermost circumferential area of ​​the centrifugal microfluidic chip for knee sports injury gene detection, and the first mixing chamber and the second mixing chamber are located in the second circumferential area of ​​the centrifugal microfluidic chip for knee sports injury gene detection Area, the first reaction chamber and the first liquid buffer chamber are located in the third circumferential area of ​​the centrifugal microfluidic chip for genetic detection of knee sports injuries, the second preset number of first liquid quantitative chambers are located in the fourth circumferential area of ​​the centrifugal microfluidic chip for genetic detection of knee sports injuries, and the second preset number of amplification detection chambers are located in the outermost circumferential area of ​​the centrifugal microfluidic chip for genetic detection of knee sports injuries. In use, the centrifugal microfluidic chip for genetic detection of knee sports injuries is mounted on a carrier, and when the carrier is running, the carrier drives the centrifugal microfluidic chip for genetic detection of knee sports injuries to rotate clockwise or counterclockwise with the preset center position as a reference.

9. The centrifugal microfluidic chip for gene detection of knee sports injuries according to claim 1, characterized in that: The center of the centrifugal microfluidic chip for genetic detection of knee sports injuries is a hollow structure, and the hollow structure is connected to the first fluid diversion channel through a main ventilation channel, and the other end of the first mixing chamber, the other end of the second mixing chamber, and the first liquid buffer chamber are connected to the main ventilation channel through a sub-ventilation channel.

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