Method and device for calibrating standard curve

Abstract

The invention discloses a calibration method and device for a standard curve, and relates to the technical field of test analysis. The method comprises the following steps: acquiring a first concentration and an actual peak area of a quality control sample; based on the first concentration and the actual peak area, constructing a quality control point corresponding to the quality control sample; and calibrating the initial standard curve through the quality control point to obtain a calibrated standard curve. In the application, the calibration of the standard curve can be realized through the quality control point, and a series of processes of configuring standard samples with different concentrations, establishing a sample introduction sequence, executing quantitative calculation and the like do not need to be executed again to reconstruct the standard curve, so that the complexity and time consumption of the calibration process of the standard curve are reduced, and the calibration of the standard curve is quickly realized; therefore, the efficiency of the whole test process is improved.

Description

Technical Field

[0001] The present application relates to the field of testing and analysis technology, and in particular to a method and device for calibrating a standard curve. Background Art

[0002] High Performance Liquid Chromatography-Triple Quadrupole Mass Spectrometry (LC-MS) combines the separation capability of HPLC with the high selectivity and flexibility of triple quadrupole mass spectrometry, and is very suitable for qualitative and quantitative analysis of organic samples. Its qualitative and quantitative analysis process includes: separation of mixed samples, ionization, mass spectrometry scanning, spectrum integration, quantitative calculation, etc. Among them, the quantitative calculation process directly affects the calculation of the concentration of the analyte.

[0003] Among them, the process of quantitative calculation is usually implemented by the external standard method. Before calculating the concentration of the analyte, it is necessary to configure standard samples of different concentrations, establish a sampling sequence, perform quantitative calculations, and a series of processes to construct a standard curve between the response values ​​(such as peak area) corresponding to the expected concentration. Subsequently, when measuring the concentration of the analyte in the unknown sample, the chromatogram corresponding to the unknown sample is obtained by liquid chromatography-mass spectrometry, and the chromatogram is integrated to obtain the corresponding peak area; based on the standard curve, the peak area is converted to the corresponding concentration.

[0004] In actual use, after the standard curve is constructed, the corresponding response value will deviate under the same concentration due to factors such as time, temperature changes, instrument drift, etc. At this time, the concentration of the unknown sample measured is still converted according to the standard curve when there is no change, resulting in a large error between the converted concentration and the actual concentration. In current technology, in order to ensure the test accuracy of unknown samples, it is necessary to rebuild the standard curve, that is, every once in a while, it is necessary to re-execute a series of processes such as configuring standard samples of different concentrations, establishing an injection sequence, and performing quantitative calculations to rebuild the standard curve and timely correct the deviation of the standard curve to ensure the accuracy of sample testing.

[0005] In summary, in the current technology, when a standard curve deviates, the standard curve needs to be reconstructed. The calibration process of the standard curve is complicated and time-consuming, and the calibration of the standard curve cannot be quickly achieved, resulting in low efficiency of the entire testing process. Summary of the invention

[0006] Based on the above problems, the present application provides a method and device for calibrating a standard curve, which reduces the complexity and time consumption of the calibration process of the standard curve, thereby quickly realizing the calibration of the standard curve, thereby improving the efficiency of the entire testing process.

[0007] The embodiments of the present application disclose the following technical solutions:

[0008] In a first aspect, the present application provides a method for calibrating a standard curve, comprising:

[0009] Obtaining a first concentration and an actual peak area of ​​a quality control sample; wherein the quality control sample is a standard sample of known concentration, the first concentration is the known concentration of the quality control sample, and the actual peak area is the area of ​​the chromatographic peak actually obtained;

[0010] Based on the first concentration and the actual peak area, construct a quality control point corresponding to the quality control sample;

[0011] The initial standard curve is calibrated through the quality control points to obtain a calibrated standard curve.

[0012] Optionally, before calibrating the initial standard curve through the quality control points to obtain a calibrated standard curve, the method further includes:

[0013] Obtaining initial discrete points corresponding to an initial standard curve; wherein the initial discrete points are discrete data points used to construct the initial standard curve, and the discrete data points are constructed based on the known concentration and corresponding peak area of ​​the standard sample;

[0014] The initial standard curve is calibrated through the quality control points to obtain a calibrated standard curve, including:

[0015] Based on the quality control points and the initial discrete points, a calibrated standard curve is obtained by refitting.

[0016] Optionally, the number of the quality control points is multiple; and the re-fitting based on the quality control points and the initial discrete points to obtain a calibrated standard curve includes:

[0017] Based on the number of the quality control points and the preset quality control point weights, the number of target discrete points is determined; wherein the preset quality control point weights are the weights of the number of quality control points to the number of all data points involved in refitting, the target discrete points are the discrete points involved in refitting among the initial discrete points, and all the data points involved in refitting include quality control points and target discrete points;

[0018] Based on the number of the target discrete points, selecting target discrete points from the initial discrete points;

[0019] Based on the quality control points and the target discrete points, a calibrated standard curve is obtained by refitting.

[0020] Optionally, the preset quality control point weight is greater than the preset discrete point weight; wherein the preset discrete point weight is the weight of the number of target discrete points to the number of all data points involved in refitting, and the sum of the preset quality control point weight and the preset discrete point weight is 1.

[0021] Optionally, after constructing the quality control point corresponding to the quality control sample based on the first concentration and the actual peak area, the method further includes:

[0022] Based on the initial standard curve, obtaining a second concentration corresponding to the actual peak area; wherein the second concentration is the concentration corresponding to the actual peak area on the initial standard curve;

[0023] determining whether the initial standard curve is valid based on a deviation between the second concentration and the first concentration;

[0024] The initial standard curve is calibrated through the quality control points to obtain a calibrated standard curve, including:

[0025] When it is determined that the initial standard curve is valid, the initial standard curve is calibrated through the quality control points to obtain a calibrated standard curve.

[0026] Optionally, the method further includes:

[0027] When it is determined that the initial standard curve is invalid, a standard curve is reconstructed based on standard samples of different concentrations.

[0028] Optionally, determining whether the initial standard curve is valid based on the deviation between the second concentration and the first concentration includes:

[0029] Determining whether a percentage deviation between the second concentration and the first concentration is less than or equal to a preset percentage;

[0030] If the percentage deviation between the second concentration and the first concentration is less than or equal to a preset percentage, determining that the standard curve is valid;

[0031] If the percentage deviation between the second concentration and the first concentration is greater than a preset percentage, the standard curve is determined to be invalid.

[0032] Optionally, the actual peak area of ​​the quality control sample is obtained by:

[0033] Obtaining a chromatogram corresponding to the quality control sample by using a high performance liquid chromatography triple quadrupole mass spectrometer;

[0034] Based on the chromatogram corresponding to the quality control sample, the actual peak area of ​​the quality control sample is obtained.

[0035] In a second aspect, the present application provides a calibration device for a standard curve, comprising:

[0036] A quality control data acquisition module, used to acquire a first concentration and an actual peak area of ​​a quality control sample; wherein the quality control sample is a standard sample of known concentration, the first concentration is the known concentration of the quality control sample, and the actual peak area is the area of ​​the chromatographic peak actually obtained;

[0037] A quality control point construction module, used to construct a quality control point corresponding to the quality control sample based on the first concentration and the actual peak area;

[0038] The standard curve calibration module is used to calibrate the initial standard curve through the quality control points to obtain a calibrated standard curve.

[0039] Optionally, the device further comprises: an initial data acquisition module;

[0040] The initial data acquisition module is used to acquire initial discrete points corresponding to the initial standard curve; wherein the initial discrete points are discrete data points used to construct the initial standard curve, and the discrete data points are constructed based on the known concentration and corresponding peak area of ​​the standard sample;

[0041] The standard curve calibration module is specifically used to re-fit the calibrated standard curve based on the quality control points and the initial discrete points.

[0042] Optionally, the number of quality control points is multiple, and the standard curve calibration module is specifically used to determine the number of target discrete points based on the number of quality control points and preset quality control point weights; based on the number of target discrete points, select target discrete points from initial discrete points; based on the quality control points and the target discrete points, re-fit to obtain a calibrated standard curve.

[0043] Optionally, the calibration device of the standard curve further includes: a comparison data acquisition module and a calibration curve validity judgment module. The comparison data acquisition module is used to obtain a second concentration corresponding to the actual peak area based on the initial standard curve; the calibration curve validity judgment module is used to determine whether the initial standard curve is valid based on the deviation between the second concentration and the first concentration;

[0044] The standard curve calibration module is specifically used to calibrate the initial standard curve through quality control points when it is determined that the initial standard curve is valid, so as to obtain a calibrated standard curve.

[0045] Optionally, the standard curve calibration device further includes: a standard curve reconstruction module, which is used to reconstruct the standard curve based on standard samples of different concentrations when it is determined that the initial standard curve is invalid.

[0046] Optionally, a calibration validity judgment module is specifically used to judge whether the percentage deviation between the second concentration and the first concentration is less than or equal to a preset percentage;

[0047] If the percentage deviation between the second concentration and the first concentration is less than or equal to a preset percentage, determining that the standard curve is valid;

[0048] If the percentage deviation between the second concentration and the first concentration is greater than a preset percentage, the standard curve is determined to be invalid.

[0049] Compared with the prior art, the present application has the following beneficial effects: the calibration of the standard curve can be achieved through quality control points, and there is no need to re-execute a series of processes such as configuring standard samples of different concentrations, establishing injection sequences, and performing quantitative calculations to reconstruct the standard curve, which reduces the complexity and time consumption of the standard curve calibration process, thereby quickly realizing the calibration of the standard curve, thereby improving the efficiency of the entire testing process. BRIEF DESCRIPTION OF THE DRAWINGS

[0050] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0051] Figure 1 A schematic diagram of a flow chart of a method for calibrating a standard curve provided in an embodiment of the present application;

[0052] Figure 2 An example diagram of an initial standard curve provided in an embodiment of the present application;

[0053] Figure 3 A schematic diagram of a process for obtaining a calibrated standard curve by refitting the quality control points and the initial discrete points provided in an embodiment of the present application;

[0054] Figure 4 A schematic diagram of a flow chart of another standard curve calibration method provided in an embodiment of the present application;

[0055] Figure 5 A schematic diagram of the structure of a standard curve calibration device provided in an embodiment of the present application. DETAILED DESCRIPTION

[0056] As described above, the standard curve is constructed through the following process: prepare a series of standard samples with different concentrations, which should cover the expected concentration range; establish an injection sequence; execute the injection sequence to collect chromatograms corresponding to all standard samples (i.e., chromatograms of the analyte in the standard sample); for each concentration point, calculate the corresponding response value (such as peak area); based on each concentration point and its corresponding response value, construct a standard curve.

[0057] In the current technology, in order to ensure the accuracy of the test of unknown samples, the above process must be re-executed at regular intervals to reconstruct a new standard curve and timely correct the deviation of the standard curve to ensure the accuracy of the sample test. However, the calibration process of the above standard curve is complicated and time-consuming, and the calibration of the standard curve cannot be quickly realized, resulting in low efficiency of the entire test process.

[0058] The present application provides a method for calibrating a standard curve, including: obtaining a first concentration and an actual peak area of ​​a quality control sample; constructing a quality control point corresponding to the quality control sample based on the first concentration and the actual peak area; calibrating the initial standard curve through the quality control point to obtain a calibrated standard curve. In the embodiment of the present application, the calibration of the standard curve can be achieved through the quality control point, and there is no need to re-execute a series of processes such as configuring standard samples of different concentrations, establishing an injection sequence, and performing quantitative calculations to reconstruct the standard curve, which reduces the complexity and time consumption of the calibration process of the standard curve, thereby quickly realizing the calibration of the standard curve, thereby improving the efficiency of the entire test process.

[0059] In order to enable those skilled in the art to better understand the solution of the present application, the technical solution in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0060] Embodiment 1:

[0061] See also Figure 1-Figure 3 , a standard curve calibration method provided in an embodiment of the present application is introduced in detail.

[0062] like Figure 1 As shown, a calibration method of a standard curve provided in an embodiment of the present application includes the following steps:

[0063] S101. Obtain a first concentration and an actual peak area of ​​a quality control sample.

[0064] Among them, quality control samples (Quality Control Samples, QC samples) are standard samples with known concentrations. In quantitative analysis, quality control samples are crucial to ensure the accuracy and reliability of the analysis results. For example, quality control samples can be used to verify whether the concentration obtained by the standard curve is accurate.

[0065] The first concentration is the known concentration of the quality control sample, and the actual peak area is the area of ​​the chromatographic peak actually obtained from the quality control sample.

[0066] In one possible implementation, the actual peak area of ​​the quality control sample is obtained as follows:

[0067] The chromatogram corresponding to the quality control sample is obtained by using a high performance liquid chromatography triple quadrupole mass spectrometer; based on the chromatogram corresponding to the quality control sample, the actual peak area of ​​the quality control sample is obtained.

[0068] The chromatogram corresponding to the quality control sample is the chromatogram of the analyte in the quality control sample.

[0069] Specifically, the chromatographic peaks in the chromatogram corresponding to the quality control sample are integrated to obtain the actual peak area of ​​the quality control sample.

[0070] S102. Constructing quality control points corresponding to the quality control sample based on the first concentration and the actual peak area.

[0071] In a possible implementation, the horizontal axis of the standard curve in the embodiment of the present application is the concentration, and the vertical axis is the sample / internal standard sample peak area ratio.

[0072] Among them, the sample / internal standard sample peak area ratio (Analyte to Internal Standard Peak Area Ratio) is an important parameter, sample / internal standard sample peak area ratio = peak area of ​​standard sample / peak area of ​​internal standard sample.

[0073] The sample can be a test sample (a sample of unknown concentration) or a standard sample (a sample of known concentration). The internal standard sample is another compound added to the standard sample, its concentration is known and does not change within the concentration range of the test substance.

[0074] By calculating the peak area ratio between the sample and the internal standard, a consistent quantitative standard can be maintained in different batches of analysis.

[0075] It can be seen that the abscissa of the quality control point corresponding to the quality control sample is the first concentration, and the ordinate of the quality control point is the ratio of the actual peak area to the peak area of ​​the internal standard sample.

[0076] S103. Calibrate the initial standard curve through the quality control points to obtain a calibrated standard curve.

[0077] The initial standard curve is a standard curve constructed in advance through a series of processes such as configuring standard samples of different concentrations, establishing an injection sequence, and performing quantitative calculations.

[0078] In a possible implementation, before performing S103, initial discrete points corresponding to the initial standard curve are obtained.

[0079] The initial discrete points are discrete data points used to construct the initial standard curve. Specifically, the discrete data points are constructed based on the known concentrations and corresponding peak areas of the standard samples. That is, in the process of constructing the initial standard curve, a series of standard samples with known concentrations are analyzed to obtain peak areas, and multiple initial discrete points for constructing the initial standard curve are constructed based on the known concentrations and their corresponding peak areas.

[0080] A standard sample refers to a sample with a known concentration (i.e., a sample containing a known concentration of the analyte), which is usually used to establish a standard curve in order to measure the concentration of the analyte in an unknown sample.

[0081] Specifically, in the embodiment of the present application, the abscissa of the initial discrete point is the known concentration, and the ordinate of the initial discrete point is the standard sample / internal standard sample peak area ratio.

[0082] For ease of understanding, the following Figure 2 Let’s take an example to introduce the initial standard curve and initial discrete points.

[0083] like Figure 2 The initial standard curve 200 shown is a standard curve that characterizes concentration and the ratio of sample / internal standard sample peak area. That is, the abscissa of the initial standard curve 200 is the concentration, and the ordinate is the ratio of sample / internal standard sample peak area, wherein the unit of concentration is mg / L (milligrams per liter).

[0084] It should be noted that the unit of concentration can be mass concentration (milligrams per liter, micrograms per milliliter, nanograms per milliliter, etc.), molar concentration (mol per liter, millimole per liter, micromole per liter, etc.), or percentage concentration (such as mass percentage, volume percentage, etc.), which is determined according to the object to be tested and is not specifically limited in this application.

[0085] Further, the initial standard curve 200 is formed by initial discrete points 201, 202, 203, 204, 205 and 206. The coordinates of the initial discrete point 201 are (50, 1.1), which characterizes a standard sample with a known concentration of 50 mg / L, and the corresponding sample / internal standard sample peak area ratio is 1.1; the coordinates of the initial discrete point 202 are (100, 2.2), which characterizes a standard sample with a known concentration of 100 mg / L, and the corresponding sample / internal standard sample peak area ratio is 2.2; the coordinates of the initial discrete point 203 are (150, 3.15), which characterizes a standard sample with a known concentration of 150 mg / L, and the corresponding sample / internal standard sample peak area ratio is 3.15 ; The coordinates of initial discrete point 204 are (200, 4.3), characterizing the standard sample with a known concentration of 200 mg / L, and the corresponding sample / internal standard sample peak area ratio is 4.3; the coordinates of initial discrete point 205 are (250, 5.25), characterizing the standard sample with a known concentration of 250 mg / L, and the corresponding sample / internal standard sample peak area ratio is 5.25; the coordinates of initial discrete point 206 are (300, 6.4), characterizing the standard sample with a known concentration of 300 mg / L, and the corresponding sample / internal standard sample peak area ratio is 6.4. Through the above discrete data points, the equation corresponding to the initial standard curve constructed is y (sample / internal standard sample peak area ratio) = 0.021x (concentration) + 0.05.

[0086] Furthermore, after obtaining the initial discrete points corresponding to the initial standard curve, the calibrated standard curve is refitted based on the quality control points and the initial discrete points. The calibration of the initial standard curve can be realized by refitting the quality control points and the initial discrete points, which reduces the complexity and time consumption of the calibration process of the standard curve, thereby quickly realizing the calibration of the standard curve and improving the efficiency of the entire test process.

[0087] It should be noted that based on the quality control points and the initial discrete points, the re-fitting of the calibrated standard curve can use linear fitting, second-order fitting, n-order fitting and other fitting methods, which are not specifically limited in this application.

[0088] For ease of understanding, the following Figure 3 , a possible implementation method of "refitting the calibrated standard curve based on quality control points and initial discrete points" is introduced in detail.

[0089] S301. Determine the number of target discrete points based on the number of quality control points and preset quality control point weights.

[0090] Among them, the number of quality control points is multiple.

[0091] The preset quality control point weight is the weight of the number of preset quality control points to the number of all data points involved in refitting. The target discrete point is the discrete point involved in refitting among the initial discrete points. All data points involved in refitting include quality control points and target discrete points.

[0092] Exemplarily, the preset quality control point weight = the number of quality control points / (the number of quality control points + the number of target discrete points). If the preset quality control point weight and the number of quality control points are known, the number of target discrete points can be obtained.

[0093] Further, the sum of the preset quality control point weight and the preset discrete point weight is 1. Exemplarily, preset discrete point weight=1-preset quality control point weight=number of target discrete points / (number of quality control points+number of target discrete points).

[0094] In a possible implementation, the preset quality control point weight is greater than the preset discrete point weight, that is, the preset quality control point weight is greater than 50%, which can further improve the accuracy and effectiveness of the standard curve calibration.

[0095] S302: Select target discrete points from the initial discrete points based on the number of target discrete points.

[0096] In a possible implementation manner, based on the number of target discrete points, target discrete points equal to the number of target discrete points are randomly selected from the initial discrete points.

[0097] S303: Based on the quality control points and the target discrete points, re-fit to obtain a calibrated standard curve.

[0098] The embodiment of the present application provides a method for calibrating a standard curve, including: obtaining a first concentration and an actual peak area of ​​a quality control sample; constructing a quality control point corresponding to the quality control sample based on the first concentration and the actual peak area; calibrating the initial standard curve through the quality control point to obtain a calibrated standard curve. In the embodiment of the present application, the calibration of the standard curve can be achieved through the quality control point, and there is no need to re-execute a series of processes such as configuring standard samples of different concentrations, establishing an injection sequence, and performing quantitative calculations to reconstruct the standard curve, which reduces the complexity and time consumption of the calibration process of the standard curve, thereby quickly realizing the calibration of the standard curve, thereby improving the efficiency of the entire test process.

[0099] Furthermore, based on the initial discrete points and the quality control points, the calibrated standard curve is refitted, which further improves the accuracy of the calibration of the initial standard curve.

[0100] Embodiment 2:

[0101] Combine the following Figure 4 , another standard curve calibration method provided in an embodiment of the present application is introduced in detail.

[0102] like Figure 4 As shown, another standard curve calibration method provided in the embodiment of the present application includes the following steps:

[0103] S401. Obtain the first concentration and actual peak area of ​​the quality control sample.

[0104] S402: Construct quality control points corresponding to the quality control sample based on the first concentration and the actual peak area.

[0105] The above S401 and S402 are the same as S101 and S102 in the first embodiment. For details on the specific implementation of S401 and S402, please refer to S101 and S102 in the first embodiment, which will not be repeated here.

[0106] S403. Based on the initial standard curve, obtain a second concentration corresponding to the actual peak area.

[0107] The second concentration is the concentration corresponding to the actual peak area on the initial standard curve.

[0108] Specifically, based on the actual peak area, the corresponding sample / internal standard sample peak area ratio is obtained, that is, the actual peak area / peak area of ​​the internal standard sample is calculated; based on the initial standard curve, the point on the initial standard curve whose y-axis is the actual peak area / peak area of ​​the internal standard sample is determined, and the x-axis of the point is the second concentration.

[0109] S404: Determine whether the initial standard curve is valid based on the deviation between the second concentration and the first concentration.

[0110] Specifically, determine whether the percentage deviation between the second concentration and the first concentration is less than or equal to a preset percentage; if the percentage deviation between the second concentration and the first concentration is less than or equal to the preset percentage, determine that the standard curve is valid; if the percentage deviation between the second concentration and the first concentration is greater than the preset percentage, determine that the standard curve is invalid.

[0111] The percentage deviation between the second concentration and the first concentration is calculated by the following formula:

[0112]

[0113] When it is determined that the standard curve is valid, proceed to S405.

[0114] When it is determined that the standard curve is invalid, proceed to S406.

[0115] S405. Calibrate the initial standard curve through the quality control points to obtain a calibrated standard curve.

[0116] The above S405 is the same as S103 in the first embodiment. For details on the specific implementation of S405, please refer to S103 in the first embodiment, which will not be repeated here.

[0117] S406. Reconstruct the standard curve based on standard samples of different concentrations.

[0118] Specifically, when it is determined that the initial standard curve is invalid, the effect of calibrating the initial standard curve only through quality control points is poor, and there will still be large errors. Therefore, a series of processes such as reconfiguring standard samples of different concentrations, establishing injection sequences, and performing quantitative calculations are performed to reconstruct the standard curve. That is, a series of standard samples with different concentrations are re-prepared, and these standard samples should cover the expected concentration range; establish injection sequences; execute injection sequences, collect chromatograms corresponding to all standard samples (i.e., chromatograms of the analytes in standard samples); for each concentration point, calculate the corresponding response value (such as peak area); based on each concentration point and its corresponding response value, construct a standard curve to ensure the test accuracy of unknown samples.

[0119] The embodiment of the present application provides a calibration method for a standard curve, including: obtaining a first concentration and an actual peak area of ​​a quality control sample; constructing a quality control point corresponding to the quality control sample based on the first concentration and the actual peak area; obtaining a second concentration corresponding to the actual peak area based on the initial standard curve; determining whether the initial standard curve is valid based on the deviation between the second concentration and the first concentration; when the initial standard curve is valid, calibrating the initial standard curve through the quality control point; when the initial standard curve is invalid, re-constructing the standard curve based on standard samples of different concentrations. That is, when the initial standard curve is valid, a fast calibration of the standard curve is performed; when the initial standard curve is invalid, no calibration is performed, but the standard curve is reconstructed. While quickly realizing the calibration of the standard curve and improving the efficiency of the entire test process, the standard curve is reconstructed for the case where the calibration effect is poor, thereby ensuring the accuracy of the test.

[0120] Embodiment three:

[0121] Combine the following Figure 5 , a standard curve calibration device provided in an embodiment of the present application is introduced in detail.

[0122] like Figure 5 As shown, a standard curve calibration device provided in an embodiment of the present application includes the following modules:

[0123] The quality control data acquisition module 501 is used to obtain the first concentration and actual peak area of ​​the quality control sample; wherein the quality control sample is a standard sample of known concentration, the first concentration is the known concentration of the quality control sample, and the actual peak area is the area of ​​the chromatographic peak actually obtained;

[0124] A quality control point construction module 502, for constructing a quality control point corresponding to the quality control sample based on the first concentration and the actual peak area;

[0125] The standard curve calibration module 503 is used to calibrate the initial standard curve through quality control points to obtain a calibrated standard curve.

[0126] In a possible implementation, the calibration device of the standard curve further includes: an initial data acquisition module, which is used to acquire initial discrete points corresponding to the initial standard curve; and a standard curve calibration module 503, which is specifically used to refit the calibrated standard curve based on the quality control points and the initial discrete points. The initial discrete points are discrete data points used to construct the initial standard curve, and the discrete data points are constructed based on the known concentration of the standard sample and the corresponding peak area.

[0127] In one possible implementation, the number of quality control points is multiple, and the standard curve calibration module 503 is specifically used to determine the number of target discrete points based on the number of quality control points and preset quality control point weights; based on the number of target discrete points, select target discrete points from initial discrete points; based on the quality control points and the target discrete points, re-fit to obtain a calibrated standard curve.

[0128] In a possible implementation, the standard curve calibration device further includes: a comparison data acquisition module and a calibration curve validity judgment module.

[0129] Among them, the comparison data acquisition module is used to obtain the second concentration corresponding to the actual peak area based on the initial standard curve; the calibration curve validity judgment module is used to determine whether the initial standard curve is valid based on the deviation between the second concentration and the first concentration; the standard curve calibration module 503 is specifically used to calibrate the initial standard curve through quality control points when it is determined that the initial standard curve is valid, so as to obtain a calibrated standard curve.

[0130] In a possible implementation, the standard curve calibration device further includes: a standard curve reconstruction module, which is used to reconstruct the standard curve based on standard samples of different concentrations when it is determined that the initial standard curve is invalid.

[0131] In a possible implementation, the calibration validity determination module is specifically used to determine whether the percentage deviation between the second concentration and the first concentration is less than or equal to a preset percentage;

[0132] If the percentage deviation between the second concentration and the first concentration is less than or equal to a preset percentage, determining that the standard curve is valid;

[0133] If the percentage deviation between the second concentration and the first concentration is greater than a preset percentage, the standard curve is determined to be invalid.

[0134] A calibration device for a standard curve provided in an embodiment of the present application includes: a quality control data acquisition module 501, which is used to obtain a first concentration and an actual peak area of ​​a quality control sample; a quality control point construction module 502, which is used to construct a quality control point corresponding to the quality control sample based on the first concentration and the actual peak area; a standard curve calibration module 503, which is used to calibrate the initial standard curve through the quality control points to obtain a calibrated standard curve. In the embodiment of the present application, the calibration of the standard curve can be achieved through the quality control points, and there is no need to re-execute a series of processes such as configuring standard samples of different concentrations, establishing an injection sequence, and performing quantitative calculations to reconstruct the standard curve, which reduces the complexity and time consumption of the calibration process of the standard curve, thereby quickly achieving the calibration of the standard curve, thereby improving the efficiency of the entire test process.

[0135] Furthermore, based on the initial discrete points and the quality control points, the calibrated standard curve is refitted, which further improves the accuracy of the calibration of the initial standard curve.

[0136] It should be noted that each embodiment in this specification is described in a progressive manner, and the same or similar parts between the embodiments can be referred to each other, and each embodiment focuses on the differences from other embodiments. In particular, for the method and device embodiments, since they are basically similar to the method embodiments, the description is relatively simple, and the relevant parts can be referred to the partial description of the method embodiments. The method and device embodiments described above are merely schematic, wherein the units described as separate components may or may not be physically separated, and the components indicated as units may or may not be physical units, that is, they may be located in one place, or they may be distributed on multiple network units. Some or all of the modules may be selected according to actual needs to achieve the purpose of the scheme of this embodiment. Ordinary technicians in this field can understand and implement it without paying creative labor.

[0137] The above is only a specific implementation of the present application, but the protection scope of the present application is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed in the present application should be included in the protection scope of the present application. Therefore, the protection scope of the present application should be based on the protection scope of the claims.

Claims

1. A method for calibrating a standard curve, characterized in that: include: Obtaining a first concentration and an actual peak area of ​​a quality control sample; wherein the quality control sample is a standard sample of known concentration, the first concentration is the known concentration of the quality control sample, and the actual peak area is the area of ​​the chromatographic peak actually obtained; Based on the first concentration and the actual peak area, construct a quality control point corresponding to the quality control sample; The initial standard curve is calibrated through the quality control points to obtain a calibrated standard curve.

2. The method according to claim 1, characterized in that Before calibrating the initial standard curve through the quality control points to obtain the calibrated standard curve, the method further includes: Obtaining initial discrete points corresponding to an initial standard curve; wherein the initial discrete points are discrete data points used to construct the initial standard curve, and the discrete data points are constructed based on the known concentration and corresponding peak area of ​​the standard sample; The initial standard curve is calibrated through the quality control points to obtain a calibrated standard curve, including: Based on the quality control points and the initial discrete points, a calibrated standard curve is obtained by refitting.

3. The method according to claim 2, characterized in that The number of the quality control points is multiple; the re-fitting based on the quality control points and the initial discrete points to obtain a calibrated standard curve includes: Based on the number of the quality control points and the preset quality control point weights, the number of target discrete points is determined; wherein the preset quality control point weights are the weights of the number of quality control points to the number of all data points involved in refitting, the target discrete points are the discrete points involved in refitting among the initial discrete points, and all the data points involved in refitting include quality control points and target discrete points; Based on the number of the target discrete points, selecting target discrete points from the initial discrete points; Based on the quality control points and the target discrete points, a calibrated standard curve is obtained by refitting.

4. The method according to claim 3, characterized in that: The preset quality control point weight is greater than the preset discrete point weight; wherein the preset discrete point weight is the weight of the number of target discrete points to the number of all data points involved in refitting, and the sum of the preset quality control point weight and the preset discrete point weight is 1.

5. The method according to claim 1, characterized in that After constructing the quality control point corresponding to the quality control sample based on the first concentration and the actual peak area, the method further includes: Based on the initial standard curve, obtaining a second concentration corresponding to the actual peak area; wherein the second concentration is the concentration corresponding to the actual peak area on the initial standard curve; determining whether the initial standard curve is valid based on a deviation between the second concentration and the first concentration; The initial standard curve is calibrated through the quality control points to obtain a calibrated standard curve, including: When it is determined that the initial standard curve is valid, the initial standard curve is calibrated through the quality control points to obtain a calibrated standard curve.

6. The method according to claim 5, characterized in that The method further comprises: When it is determined that the initial standard curve is invalid, a standard curve is reconstructed based on standard samples of different concentrations.

7. The method according to claim 5, characterized in that The step of determining whether the initial standard curve is valid based on the deviation between the second concentration and the first concentration comprises: Determining whether a percentage deviation between the second concentration and the first concentration is less than or equal to a preset percentage; If the percentage deviation between the second concentration and the first concentration is less than or equal to a preset percentage, determining that the standard curve is valid; If the percentage deviation between the second concentration and the first concentration is greater than a preset percentage, the standard curve is determined to be invalid.

8. The method according to claim 1, characterized in that The actual peak area of ​​the quality control sample is obtained by the following method: Obtaining a chromatogram corresponding to the quality control sample by using a high performance liquid chromatography triple quadrupole mass spectrometer; Based on the chromatogram corresponding to the quality control sample, the actual peak area of ​​the quality control sample is obtained.

9. A calibration device for a standard curve, characterized in that: include: A quality control data acquisition module, used to acquire a first concentration and an actual peak area of ​​a quality control sample; wherein the quality control sample is a standard sample of known concentration, the first concentration is the known concentration of the quality control sample, and the actual peak area is the area of ​​the chromatographic peak actually obtained; A quality control point construction module, used to construct a quality control point corresponding to the quality control sample based on the first concentration and the actual peak area; The standard curve calibration module is used to calibrate the initial standard curve through the quality control points to obtain a calibrated standard curve.

10. The device according to claim 9, characterized in that The device also includes: an initial data acquisition module; The initial data acquisition module is used to acquire initial discrete points corresponding to the initial standard curve; wherein the initial discrete points are discrete data points used to construct the initial standard curve, and the discrete data points are constructed based on the known concentration and corresponding peak area of ​​the standard sample; The standard curve calibration module is specifically used to re-fit the calibrated standard curve based on the quality control points and the initial discrete points.

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