Solid nucleic acid register and data registering method

Abstract

The invention relates to the field of DNA calculation, and discloses a solid-state nucleic acid register and a data registering method, the solid-state nucleic acid register comprises a solid-phase carrier and one or more planar origami structures fixed on the surface of the solid-phase carrier; wherein the main surface of the planar origami structure and the main surface of the solid-phase carrier are in solid-phase connection to form a fixed relationship, and the other main surface of the planar origami structure is configured to write, store, read and rewrite data; and the planar origami structure is formed by complementary pairing and self-assembly of bases. The data registering method comprises the following steps: preparing the solid nucleic acid register, and constructing a solid nucleic acid reaction tank; adding a DNA calculation circuit assembly into the solid nucleic acid reaction tank and setting a data reading device; and writing, storing, reading and rewriting data of the DNA calculation circuit in the solid nucleic acid reaction tank, and observing by adopting a data reading device. The compact sequential digital circuit is provided, and the information processing efficiency is improved.

Description

Technical Field

[0001] The present application relates to the field of DNA computing technology, and more specifically to a solid-state nucleic acid register and a method for data storage. Background Art

[0002] In the field of modern computing, DNA computing, as an emerging computing model, has attracted widespread attention due to its unique Watson-Crick base pairing characteristics. This is an innovative method that uses the chemical properties and biological reactions of DNA molecules for information processing and computing. Its core lies in its ability to achieve complex logical operations and data storage through precise interactions between molecules. This computing model can not only process large amounts of data in parallel, but also perform efficient information processing at the microscopic scale.

[0003] The advantages of DNA computing are mainly reflected in its high-density storage capacity and biocompatibility. Compared with traditional electronic computers, DNA molecules can store massive amounts of information in a smaller volume, which provides a new approach to solving large-scale data problems. In addition, DNA computing systems show superior energy efficiency in certain application scenarios because they can perform calculations at room temperature, reducing energy consumption.

[0004] In recent years, researchers have developed a variety of functions and applications using the precise molecular interactions of DNA molecules, including logic circuits, neural network computing, and medical diagnosis. These innovations demonstrate the great potential of DNA computing in solving complex problems, especially in parallel processing and efficient storage. However, DNA computing also faces challenges, such as slow response speed, high error rate, and insufficient system scalability. These limitations affect the practicality of existing DNA computing technology in handling complex tasks.

[0005] At the same time, DNA computing also needs to implement sequential computing (an important component of information processing), which enables the system to efficiently process and transmit information by stepping through instructions or operations. Sequential computing is an important component of information processing, especially in classical computers and biological systems. In biology, cells regulate complex biological processes through the sequential expression of genes, and in computer science, the operation of electronic computers also relies on the processing of sequential information.

[0006] At present, although a variety of DNA-based sequential computing systems have been proposed, such as timers, oscillators, and state machines, these systems still face significant limitations when performing complex digital calculations. Existing DNA computing technology can often only handle relatively simple logical operations and is difficult to cope with higher-order complex computing tasks. Moreover, existing systems usually take a long time in the process of information transmission. For example, the reading, writing, and transmission of data often rely on slow chemical reactions and physical movements, which limits the breadth and practicality of its practical applications. Even in the context of rapid development of research, how to effectively improve the speed and complexity of DNA computing is still a major challenge in current research. This not only affects the theoretical application of DNA computing, but also restricts its promotion and implementation in practical scenarios. Therefore, in-depth exploration and overcoming these limitations will play a key role in promoting the development of DNA computing.

[0007] In summary, existing DNA computing technologies face a series of limitations when processing complex tasks, including slow response speed, high error rate, and insufficient system scalability. This means that these systems can usually only perform relatively simple logical operations and cannot effectively cope with higher-order complex computing tasks. In addition, existing sequential computing systems often take a long time to read, write, and transmit data, which affects their feasibility in practical applications. Summary of the invention

[0008] The purpose of the present application is to provide a solid-state nucleic acid register and a method for data storage, wherein the register and the circuit provide a compact sequential digital circuit and improve the efficiency of information processing.

[0009] The present application discloses a solid-state nucleic acid register, comprising:

[0010] A solid support, and one or more planar origami structures fixed on the surface of the solid support;

[0011] The main surface of the planar origami structure and the main surface of the solid phase carrier are solid-phase connected to form a fixed relationship, and the other main surface of the planar origami structure is configured to write, store, read and rewrite data; and

[0012] The planar origami structure is formed by self-assembly through complementary base pairing.

[0013] In a preferred embodiment, it includes:

[0014] A fixed chain is provided at a specific position on the main surface of the planar origami structure, and the fixed chain is used to form a fixed connection with the molecules on the solid phase carrier;

[0015] A read-write chain is provided at a specific position on another main surface of the planar origami structure, and the read-write chain is used for writing, storing, reading and rewriting data chains.

[0016] In a preferred embodiment, it includes:

[0017] There are multiple channels or multiple regularly arranged areas on the planar origami structure, and each channel or each area is used for independent writing, storage, reading and rewriting of one bit of data.

[0018] The present application also discloses a method for data storage, comprising:

[0019] Step 101. Prepare the solid nucleic acid register as described above and construct a solid nucleic acid reaction tank;

[0020] Step 102. Adding a DNA computing circuit component to the solid nucleic acid reaction tank and setting a data reading device;

[0021] Step 103. Data writing, storage, reading and rewriting of the DNA computing circuit are performed in the solid-state nucleic acid reaction tank, and observation is performed using the data reading device.

[0022] In a preferred embodiment, step 101 further includes:

[0023] Step 201. Cleaning, drying, modifying and storing the solid phase carrier for subsequent use;

[0024] Step 202. Add a molecular solution that can react with the fixed chain to form a fixed connection on the solid phase carrier and incubate for a period of time;

[0025] Step 203. Afterwards, the planar origami structure is added to the solid phase carrier to form the solid nucleic acid register, thereby completing the preparation of the solid nucleic acid reaction tank, wherein the solid nucleic acid reaction tank is a reaction environment for data storage.

[0026] In a preferred embodiment, the step 103 of writing, storing, reading and rewriting data of the DNA computing circuit in the solid nucleic acid reaction tank further includes:

[0027] Step 301. Perform calculations on a solution phase DNA circuit and write calculation results;

[0028] Step 302. Read out the calculation results stored in the solid-state nucleic acid register;

[0029] Step 303: Perform asynchronous serial connection of DNA circuits and write new calculation results.

[0030] In a preferred example, step 301 further includes:

[0031] Adding the first input nucleic acid strand of the added DNA computing circuit component into the solid nucleic acid reaction tank;

[0032] After a period of reaction, the DNA computing circuit component and the first input nucleic acid chain generate first output data, and then a chain displacement reaction is performed. The first output data is stored in the corresponding area of ​​the solid-state nucleic acid register and saved as a first data chain.

[0033] In a preferred example, step 302 further includes:

[0034] Adding a first release nucleic acid chain for reading stored data into a solid nucleic acid reaction tank that already stores calculation results;

[0035] The first data chain on the solid-state nucleic acid register is released into the solution by a chain substitution reaction through the first released nucleic acid chain, thereby completing the reading of the first output data of the DNA computing circuit component.

[0036] In a preferred embodiment, step 303 further includes:

[0037] Adding a corresponding downstream DNA circuit component to the solid nucleic acid reaction tank containing the released first output data to generate second output data;

[0038] Then, through a chain displacement reaction, the second output data is stored in a corresponding area of ​​the solid-state nucleic acid register and saved as a second data chain.

[0039] In a preferred embodiment, the observation using the data reading device in step 103 further includes:

[0040] By fluorescently marking the data chain corresponding to the calculation result generated in each step and using a total internal reflection fluorescence microscope to record the change of the fluorescence value in real time, a visual readout of the calculation result of the series circuit is achieved, wherein the data reading device includes the total internal reflection fluorescence microscope.

[0041] Compared with the prior art, the main differences and effects of the embodiments of the present application are:

[0042] A compact sequential digital circuit was constructed, which improved the efficiency of information processing and achieved high-speed data transmission in sequential nucleic acid circuits.

[0043] It enhances the stability of the system, reduces the impact of external interference on system accuracy, and reduces the error rate.

[0044] It has expanded computing power and can support more complex logical operations, enabling DNA computing technology to cope with higher-order complex computing tasks and expand its scope of application.

[0045] It saves space and resources, reduces the required physical space and material consumption, and improves the overall efficiency of the system.

[0046] The specification of the present application records a large number of technical features, which are distributed in various technical solutions. If all possible combinations of technical features of the present application (i.e., technical solutions) are to be listed, the specification will be too long. In order to avoid this problem, the various technical features disclosed in the above-mentioned invention content of the present application, the various technical features disclosed in the various embodiments and examples below, and the various technical features disclosed in the accompanying drawings can be freely combined with each other to form various new technical solutions (these technical solutions should all be regarded as having been recorded in this specification), unless the combination of such technical features is technically infeasible. For example, in one example, feature A+B+C is disclosed, and in another example, feature A+B+D+E is disclosed, and features C and D are equivalent technical means that play the same role. Technically, only one can be used, and it is impossible to use them at the same time. Feature E can be combined with feature C technically. Then, the solution of A+B+C+D should not be regarded as having been recorded because it is technically infeasible, and the solution of A+B+C+E should be regarded as having been recorded. BRIEF DESCRIPTION OF THE DRAWINGS

[0047] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0048] Figure 1 This is a structural characterization result of a solid-state nucleic acid register in one embodiment of the present application.

[0049] Figure 2 This is based on the application of multiple storage sites in this application.

[0050] Figure 3 This is based on the application method of the chip bin in this application.

[0051] Figure 4 The method is based on writing and reading the results of a solution phase DNA computing circuit in one embodiment of the present application;

[0052] Figure 5 It is a record of calculation results of solution phase DNA calculation sequence in one embodiment of the present application. DETAILED DESCRIPTION

[0053] In the following description, many technical details are provided to help readers better understand the present application. However, those skilled in the art can understand that the technical solution claimed in the present application can be implemented even without these technical details and various changes and modifications based on the following embodiments.

[0054] Description of some concepts:

[0055] Solution-phase DNA circuit: A circuit system constructed using DNA molecules in a solution environment. Unlike traditional electronic circuits based on solid-state materials such as silicon, it uses DNA molecules as the main building blocks in a liquid solution phase, and realizes circuit functions such as information storage, signal processing and transmission through interactions between DNA molecules (such as complementary base pairing).

[0056] Register: A component of the central processing unit (CPU). It is a high-speed storage component with limited storage capacity that can be used to temporarily store instructions, data, and addresses. It is like a small "workroom" of the CPU and plays a key role in the computer's data processing process.

[0057] In order to make the objectives, technical solutions and advantages of the present application clearer, the implementation methods of the present application will be further described in detail below in conjunction with the accompanying drawings.

[0058] The present application discloses a solid-state nucleic acid register, such as Figure 1 As shown in, including:

[0059] A solid phase carrier, and one or more planar origami structures fixed on the surface of the solid phase carrier; wherein the main surface of the planar origami structure and the main surface of the solid phase carrier are solid-phase connected to form a fixed relationship, and the other main surface of the planar origami structure is configured to write, store, read and rewrite data; and the planar origami structure is self-assembled by base complementary pairing.

[0060] In this solid-state nucleic acid register design, compact sequential digital circuits are constructed by resetting the states of fixed solid-state nucleic acid registers rather than physically moving them. Using rewritable solid-state nucleic acid registers attached to a solid phase carrier, the three-dimensional circuit output is compressed onto a two-dimensional surface to improve the efficiency of information processing. After the circuit is updated, the data stored on the surface can be quickly read and transferred to the computing system, thereby achieving high-speed data transmission of sequential nucleic acid circuits.

[0061] In one embodiment, it includes:

[0062] A fixed chain is provided at a specific position on the main surface of the planar origami structure, and the fixed chain is used to form a fixed connection with the molecule on the solid phase carrier;

[0063] A read-write chain is arranged at a specific position on another main surface of the planar origami structure, and the read-write chain is used for writing, storing, reading and rewriting the data chain.

[0064] Among them, fixed chain and read-write chain are both staple chains.

[0065] In one embodiment, it includes:

[0066] There are multiple channels or multiple regularly arranged areas on the planar origami structure, and each channel or each area is used for independent writing, storage, reading and rewriting of one bit of data.

[0067] like Figure 2 As shown, in one application, a single solid-state nucleic acid register can have more than 200 addressable storage sites. Each storage site consists of a corresponding write area, address information, and read area. By artificially designing DNA sequences, the possibility of single-molecule information storage in multiple regions and multiple sites can be realized.

[0068] like Figure 3 As shown, in one application mode, combined with microfluidics, different chip compartments are manually printed to successfully realize the order of different DNA computing and high-throughput large-scale DNA computing.

[0069] The advantages of the solid-state nucleic acid register design in this application include:

[0070] Improved computing speed: By eliminating physical movement operations, the time for information transmission is significantly shortened, improving the overall computing speed.

[0071] Enhanced system stability: Fixed solid-state nucleic acid registers remain immobile throughout the calculation process, reducing the impact of external interference on system accuracy and reducing error rates.

[0072] The present application also discloses a method for data storage, such as Figure 4 As shown, including:

[0073] Step 101. Prepare the solid nucleic acid register as described above and construct a solid nucleic acid reaction tank; which includes pre-treating the solid phase carrier. In the process of constructing the solid nucleic acid register, pre-treating the solid phase carrier is an important step to ensure that the planar origami structure can be effectively attached.

[0074] Step 102. Adding a DNA computing circuit component to the solid nucleic acid reaction tank and setting a data reading device;

[0075] Step 103. Data writing, storage, reading and rewriting of the DNA computing circuit are performed in the solid-state nucleic acid reaction tank, and observation is performed using a data reading device.

[0076] in, Figure 4 A in FIG. 1 is a schematic diagram of writing and reading data from a solid-state nucleic acid register. Figure 4 B in the figure shows the various fluorescence changes during the process of DNA data writing and reading. Figure 4C in the figure is a fluorescence image of a single solid-state nucleic acid register at a specified time point over time, wherein the blue channel shows the position of the solid-state nucleic acid register labeled with fluorescent ATTO-488, and the red channel shows the fluorescence of the Cy5 labeled data chain.

[0077] In one embodiment, step 101 further includes:

[0078] Step 201. Clean, dry, modify and store the solid phase carrier for subsequent use;

[0079] Step 202. Add a molecule solution that can react with the fixed chain to form a fixed connection on the solid phase carrier and incubate for a period of time;

[0080] Step 203. Afterwards, a planar origami structure is added to the solid phase carrier to form a solid nucleic acid register, thereby completing the preparation of the solid nucleic acid reaction tank, wherein the solid nucleic acid reaction tank is a reaction environment for data storage.

[0081] In one embodiment, the step 103 of writing, storing, reading and rewriting data of the DNA computing circuit in the solid nucleic acid reaction tank further includes:

[0082] Step 301. Perform calculations on a solution phase DNA circuit and write calculation results;

[0083] Step 302. Read out the calculation results stored in the solid-state nucleic acid register;

[0084] Step 303: Perform asynchronous serial connection of DNA circuits and write new calculation results.

[0085] In one embodiment, step 301 further includes:

[0086] Adding a first input nucleic acid chain (input) of the added DNA computing circuit component into the solid nucleic acid reaction tank;

[0087] After a period of reaction, the first output data is generated by the DNA computing circuit component and the first input nucleic acid chain, and then a chain displacement reaction is performed, and the first output data is stored in a corresponding area of ​​the solid-state nucleic acid register and saved as a first data chain;

[0088] Then rinse away the excess liquid with the reaction solution.

[0089] In one embodiment, step 302 further includes:

[0090] Adding a first release nucleic acid chain (release) for reading stored data into the solid nucleic acid reaction tank where the calculation results have been stored;

[0091] The first data chain on the solid nucleic acid register is released into the solution by a chain substitution reaction through the first released nucleic acid chain, thereby completing the reading of the first output data of the DNA computing circuit component.

[0092] In one embodiment, step 303 further includes:

[0093] Adding a corresponding downstream DNA circuit component to the solid nucleic acid reaction tank containing the released first output data to generate second output data;

[0094] Then, through the chain displacement reaction, the second output data is stored in the corresponding area of ​​the solid-state nucleic acid register and saved as the second data chain.

[0095] In one embodiment, the observation using the data reading device in step 103 further includes:

[0096] By fluorescently marking the data chain corresponding to the calculation result generated in each step and using a total internal reflection fluorescence microscope (TIRF) to record the change of the fluorescence value in real time, a visual readout of the calculation result of the series circuit is achieved, wherein the data reading device includes a total internal reflection fluorescence microscope.

[0097] The operation result is as follows Figure 5 As shown, Figure 5 A in the figure represents a multi-layer DNA circuit connected in series. Figure 5 B in the figure indicates that different regions on a single solid-state nucleic acid register can store different DNA computing results. Figure 5 C in the figure is the truth table of the designed DNA sequential circuit. Figure 5 D in is the calculation result of the first layer of operation circuit. Figure 5 The E in is the operation result of the entire circuit.

[0098] The advantages of the data storage method in this application include:

[0099] Expanded computing power: The architecture can support more complex logical operations, enabling DNA computing technology to handle higher-order complex computing tasks and expand its scope of application.

[0100] Save space and resources: By compressing the 3D circuit output onto a 2D surface, the required physical space and material consumption are reduced, improving the overall efficiency of the system.

[0101] In one embodiment, the planar origami structure in the solid nucleic acid register is a square, the solid phase carrier is a glass substrate, and the solid phase connection is formed by a binding reaction between biotin and streptavidin. Step 101 further includes:

[0102] Step 401. Multiple planar origami structures with square shapes are formed through complementary base pairing; the use of classic square origami as the basic model of the solid nucleic acid register is based on the physical properties of various DNA origami that have been successfully constructed and the needs of experiments;

[0103] Step 402. A fixed chain that can hybridize with the supporting short chain modified with biotin is arranged at a specific position on the main surface of the planar origami structure, and the extended portion of the fixed chain is exposed below the bottom surface to ensure that it can subsequently be stably hybridized with the supporting short chain modified with biotin;

[0104] Step 403: Arrange a read / write chain at a specific position of another main surface of the planar origami structure, and expose the extended portion of the read / write chain on the surface to ensure that the data chain can be stored at a specific address in a stable hybridization manner;

[0105] Step 404. A solid nucleic acid register is obtained by annealing assembly and polyethylene glycol purification. The staple chain combination with the address design is mixed with the backbone chain, and an addressable initial solid nucleic acid register is formed by annealing assembly. The DNA origami assembly process is referred to Rothemund, PWK Folding DNA to Create Nanoscale Shapes and Patterns. Nature 2006, 440 (7082), 297-302. Before use, the assembled solid nucleic acid register is PEG-purified, and a biotin-modified supporting DNA short chain and a closed DNA short chain for pre-sealing the storage information position are added thereto. After incubation at room temperature for 2 hours, polyethylene glycol (PEG) purification is performed again.

[0106] In one embodiment, for the convenience of observation, a glass substrate is selected as a solid phase carrier, and step 201 further includes:

[0107] Step 501. Use an ultrasonic cleaner and a cleaning agent to clean the glass substrate to remove surface contaminants: The glass substrate needs to be thoroughly cleaned by using an ultrasonic cleaner with an appropriate cleaning agent (such as a mixture of deionized water and ethanol) to remove dust, grease and other contaminants that may exist on the surface;

[0108] Step 502: Soaking the cleaned glass substrate in a sodium hydroxide solution: wherein the cleaned glass substrate needs to be soaked in a sodium hydroxide solution for further surface activation to improve its ability to bind to biomolecules;

[0109] Step 503: drying the glass substrate after being activated by sodium hydroxide; placing the treated glass substrate in a silane solution for incubation to alkylate the surface of the glass substrate;

[0110] Step 504: Dry the surface alkylated glass substrate; after the incubation, rinse the glass substrate gently with deionized water and blow dry with high-purity nitrogen. Then put it in a high-temperature oven to completely dry the glass substrate, and finally store it in anhydrous ethanol to ensure that no water molecules adhere to the surface of the glass substrate during the process.

[0111] In one embodiment, for the convenience of observation, a glass substrate is selected as a solid phase carrier, and the solid phase connection is formed by a binding reaction between biotin and streptavidin. Step 202 also includes:

[0112] Step 601. Add polyethylene glycol or biotin-polyethylene glycol solution (PEG / Biotin-PEG solution) to the glass substrate and incubate for a period of time (usually 2 hours) to allow the Biotin-PEG molecules to fully react with the surface of the glass substrate and be fixed; after the incubation, rinse with the reaction solution to remove the free excess PEG / Biotin-PEG molecules in the solid nucleic acid reaction tank;

[0113] Step 602. Then, add an appropriate amount of streptavidin, so that the streptavidin molecules can bind to the biotin molecules on the Biotin-PEG, and incubate again for a period of time; then use the reaction solution to wash away the free excess streptavidin molecules;

[0114] Step 603. Then, add a sufficient amount of solid nucleic acid registers to the glass substrate, incubate at room temperature, and rinse again with the reaction solution; through the above steps, the solid nucleic acid registers are fixed on the glass substrate, and the solid nucleic acid reaction tank is constructed, which can provide a stable and controllable reaction environment.

[0115] In one embodiment, a read-write chain is provided at a specific position on a main surface of the planar origami structure, and step 103 further includes:

[0116] When performing a write operation, the first output data undergoes a chain replacement reaction with the read-write chain;

[0117] When performing a read operation, the first released nucleic acid strand undergoes a strand release reaction with the read-write strand.

[0118] It should be noted that in the claims and description of this patent, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "including one" do not exclude the existence of other identical elements in the process, method, article or device including the elements.

[0119] All documents mentioned in this application are cited as references in this application, just as each document is cited as reference individually. In addition, it should be understood that after reading the above teaching content of this application, those skilled in the art can make various changes or modifications to this application, and these equivalent forms also fall within the scope defined by the claims attached to this application.

[0120] In some cases, the actions or steps described in the claims may be performed in a different order than in the embodiments and still achieve the desired results. In addition, the processes depicted in the drawings do not necessarily require the specific order or sequential order shown to achieve the desired results. In some embodiments, multitasking and parallel processing are also possible or may be advantageous.

Claims

1. A solid-state nucleic acid register, characterized in that: include: A solid support, and one or more planar origami structures fixed on the surface of the solid support; Among them, the main surface of the planar origami structure and the main surface of the solid phase carrier are solid-phase connected to form a fixed relationship, and the other main surface of the planar origami structure is configured to write, store, read and rewrite data; and the planar origami structure is formed by self-assembly through base complementary pairing.

2. The solid-state nucleic acid register according to claim 1, characterized in that: include: A fixed chain is provided at a specific position on the main surface of the planar origami structure, and the fixed chain is used to form a fixed connection with the molecules on the solid phase carrier; A read-write chain is provided at a specific position on another main surface of the planar origami structure, and the read-write chain is used for writing, storing, reading and rewriting data chains.

3. The solid-state nucleic acid register according to claim 1, characterized in that: include: There are multiple channels or multiple regularly arranged areas on the planar origami structure, and each channel or each area is used for independent writing, storage, reading and rewriting of one bit of data.

4. A method for data storage, characterized in that: include: Step 1 01. Prepare the solid nucleic acid register as described in any one of claims 1 to 3, and construct a solid nucleic acid reaction tank; Step 102. Adding a DNA computing circuit component to the solid nucleic acid reaction tank and setting a data reading device; Step 103. Data writing, storage, reading and rewriting of the DNA computing circuit are performed in the solid-state nucleic acid reaction tank, and observation is performed using the data reading device.

5. The method according to claim 4, characterized in that Step 101 also includes: Step 201. Cleaning, drying, modifying and storing the solid phase carrier for subsequent use; Step 202. Add a molecular solution that can react with the fixed chain to form a fixed connection on the solid phase carrier and incubate for a period of time; Step 203. Afterwards, the planar origami structure is added to the solid phase carrier to form the solid nucleic acid register, thereby completing the preparation of the solid nucleic acid reaction tank, wherein the solid nucleic acid reaction tank is a reaction environment for data storage.

6. The method according to claim 4, characterized in that The step 103 of writing, storing, reading and rewriting data of the DNA computing circuit in the solid nucleic acid reaction tank also includes: Step 301. Perform calculations on a solution phase DNA circuit and write calculation results; Step 302. Read out the calculation results stored in the solid-state nucleic acid register; Step 303: Perform asynchronous serial connection of DNA circuits and write new calculation results.

7. The method according to claim 6, characterized in that Step 301 also includes: Adding the first input nucleic acid strand of the added DNA computing circuit component into the solid nucleic acid reaction tank; After a period of reaction, the DNA computing circuit component and the first input nucleic acid chain generate first output data, and then a chain displacement reaction is performed. The first output data is stored in the corresponding area of ​​the solid-state nucleic acid register and saved as a first data chain.

8. The method according to claim 6, characterized in that Step 302 also includes: Adding a first release nucleic acid chain for reading stored data into a solid nucleic acid reaction tank that already stores calculation results; The first data chain on the solid-state nucleic acid register is released into the solution by a chain substitution reaction through the first released nucleic acid chain, thereby completing the reading of the first output data of the DNA computing circuit component.

9. The method according to claim 6, characterized in that Step 303 also includes: Adding a corresponding downstream DNA circuit component to the solid nucleic acid reaction tank containing the released first output data to generate second output data; Then, through a chain displacement reaction, the second output data is stored in a corresponding area of ​​the solid-state nucleic acid register and saved as a second data chain.

10. The method according to claim 4, characterized in that The step 103 of using the data reading device to perform observation further includes: By fluorescently marking the data chain corresponding to the calculation result generated in each step and using a total internal reflection fluorescence microscope to record the change of the fluorescence value in real time, a visual readout of the calculation result of the series circuit is achieved, wherein the data reading device includes the total internal reflection fluorescence microscope.

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