Pesticide composition containing tiadinil

Abstract

The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition containing tiadinil. The pesticide composition containing the tiadinil is formed by compounding the tiadinil and the fluorine azole activated ester or the magnolol, and the mass ratio of the tiadinil to the fluorine azole activated ester or the magnolol is (1-500): (500-1). The tiadinil and the fluorine azole activated ester or the magnolol are compounded, a synergistic effect is shown, and compared with a single agent, the pesticide composition can improve the control effect on plant diseases, reduce the field dosage and effectively reduce environmental pollution and pesticide residues. The pesticide composition disclosed by the invention contains two effective components, is beneficial to avoiding and delaying generation of drug resistance of plant pathogenic bacteria, and can reduce the risk of drug resistance compared with a single agent.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition containing thiamethoxam. Background Art

[0002] Thiacinamide (CAS: 223580-51-6) is a low-toxic pesticide with poor inhibitory or eradication activity against pathogens that have already invaded and developed, but it can induce systemic resistance in rice, prevent pathogen hyphae from invading adjacent healthy plant tissues, and induce the production of disease-resistant genes; in addition, thiamethoxam has good systemicity, can be absorbed through the roots, and quickly transmitted to various parts of the plant body, with a long lasting effect, and can play a good role in hindering the development of diseases. Thiacinamide has a wide spectrum of action and can also be used for the comprehensive prevention and control of powdery mildew and crown rust in cereals such as barley, wheat and oats, as well as the comprehensive prevention and control of gaseous plant rust, late blight, downy blight, black spot, bacterial spot disease, soft rot, etc. However, the cost of using thiamethoxam alone is high and there is a risk of drug resistance.

[0003] Fluorazole activated ester FBT is a plant-induced disease resistance activator synthesized by East China University of Science and Technology. Fluorazole activated ester can induce the expression of a series of genes related to disease resistance, thereby inducing the production of plant disease resistance. In addition, it can promote the synthesis of various proteins related to disease resistance, significantly increase the activity of a series of enzymes related to disease resistance, and at the same time increase the activity of various peroxidases related to plant stress resistance. Secondly, fluorazole activated ester can induce the accumulation of a thicker callose protective layer on the plant cell wall at the site of fungal invasion, thereby preventing fungal invasion in the cell structure.

[0004] Magnolol is a natural compound extracted from the dried bark, root bark and branch bark of Magnolia officinalis or Magnolia officinalis of the Magnoliaceae family. The prior art reports that magnolol exhibits effective antibacterial activity against Rhizoctonia solani, Sclerotinia sclerotiorum of rape, Botrytis cinerea of ​​tomato, Fusarium wilt of cotton, Fusarium head blight of wheat and Magnaporthe oryzae. Compared with traditional chemical synthetic pesticides, magnolol has the advantages of low toxicity, high selectivity and high environmental compatibility. It has broad application prospects in agricultural disease prevention and control. It can not only be used as an effective antibacterial agent, but also as a candidate substance for the development of new plant-based green pesticides.

[0005] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention and should not be regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art. Summary of the invention

[0006] The object of the present invention is to provide a pesticide composition containing thiazolamide, so as to solve the problem that thiazolamide has high cost and the risk of drug resistance when used alone.

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] A pesticide composition containing thiazinamide is prepared by compounding thiazinamide with fluazifop-butyl or magnolol; the mass ratio of thiazinamide to fluazifop-butyl or magnolol is 1-500:500-1.

[0009] Furthermore, the mass ratio of the thiazide to the fluazifop-butyl activated ester is 1-40:10-1.

[0010] Furthermore, the mass ratio of the thiazide to the fluazifop-butyl activated ester is 1-5:3-1.

[0011] Furthermore, the mass ratio of thiazide to magnolol is 1-20:20-1.

[0012] Furthermore, the mass ratio of thiazide to magnolol is 10:1.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] (1) The compounding of thiamethoxam with fluazifop or magnolol of the present invention exhibits a synergistic effect. Compared with a single agent, the pesticide composition of the present invention can improve the control effect of plant diseases, reduce the amount of pesticide used in the field, and effectively reduce environmental pollution and pesticide residues.

[0015] (2) The pesticide composition of the present invention contains two active ingredients, which is beneficial to avoid and delay the development of drug resistance in plant pathogens and can reduce the risk of drug resistance compared with a single agent. DETAILED DESCRIPTION

[0016] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is described with the following specific embodiments, but the present invention is by no means limited to these examples.

[0017] Bioassay Examples

[0018] 1. Test crops

[0019] Select "Y Liangyou 17" rice seeds with full grains, soak and disinfect them in 1% sodium hypochlorite solution for 30 minutes, rinse them repeatedly with sterile water for 3 times, place them in a constant temperature incubator at 27℃ to germinate and whiten, and sow them in seedling trays filled with sterilized matrix. When the seedlings grow to the four-leaf and one-heart stage, retain 15 seedlings with basically the same growth in each seedling tray for subsequent inoculation experiments.

[0020] 2. Test spore suspension

[0021] Rice blast fungus (Pyricularia oryzae) was isolated from infected rice plants in rice fields.

[0022] The rice blast fungus was inoculated on oat culture medium. When the pathogen grew conidia, the conidia were washed with sterile water and filtered with double-layer gauze to prepare 1×10 5 Prepare a spore suspension with a concentration of spores / mL for later use.

[0023] 3. Test reagents

[0024] 99% thiamethoxam, 98% fluazifop-activated ester, 98% magnolol

[0025] 4. Test method (refer to "NY / T 1156.8-2007 Pesticide Indoor Biological Test Test Guidelines Fungicide Part 8: Pot Plant Method for Control of Rice Blast")

[0026] The test agent was dissolved in dimethyl sulfoxide and then diluted with 0.1% Tween-80 aqueous solution to form a single-dose mother solution, and multiple groups of ratios were set; then the single-dose mother solution and the ratio mixture were diluted with 0.1% Tween-80 aqueous solution to form 6 mass concentration gradients. The liquid was sprayed on the leaves of the test crop until they were completely wet, and naturally air-dried. One seedling tray was treated for each time, and 4 times were repeated, and the treatment of spraying 0.1% Tween-80 aqueous solution was set as a blank control. After 24 hours, the test spore suspension was sprayed and inoculated on the test crop treated with the agent. After inoculation, it was transferred to a dark condition with a temperature of 25°C and a relative humidity of 95% for moisturizing culture for 24 hours, and then transferred to a temperature of 25°C, 12 hours of light and dark alternation, a light intensity of 20000lx, and a relative humidity of 90% for continued culture.

[0027] When the diseased leaf rate of the blank control reaches more than 50%, the disease situation of the leaves of the whole plant is investigated in stages, and the disease index and control effect of each treatment are calculated based on the investigation data.

[0028] 5. Grading Standards

[0029] Level 0: The whole plant is disease-free;

[0030] Level 1: Brown spots appear;

[0031] Level 3: Typical spindle-shaped lesions appear, and the lesion area accounts for less than 5% of the entire leaf area;

[0032] Level 5: Typical spindle-shaped lesions appear, and the lesion area accounts for 6-25% of the entire leaf area;

[0033] Level 7: Typical spindle-shaped lesions appear, and the lesion area accounts for 26-50% of the entire leaf area;

[0034] Level 9: Typical spindle-shaped spots appear, and the area of ​​the spots accounts for more than 50% of the total leaf area.

[0035] 6. Calculation method

[0036]

[0037] Where:

[0038] X——disease index;

[0039] N i ——Number of diseased leaves at each level;

[0040] i——relative level value;

[0041] N——Total number of leaves surveyed.

[0042]

[0043] Where:

[0044] P——control effect, in percentage (%);

[0045] CK——blank control disease index;

[0046] PT - drug treatment index.

[0047] 7. Data Analysis

[0048] DPS software was used to perform regression analysis on the logarithmic value of each treatment agent concentration and the probability value of control effect, and the EC of each treatment agent was calculated. 50 The Sun Yunpei method was used to calculate the co-toxicity coefficient (CTC value) of the mixture. The synergistic effect of the drugs was evaluated based on the calculated co-toxicity coefficient (CTC). CTC≤80 was antagonistic, 80<CTC<120 was additive, and CTC≥120 was synergistic. The results are shown in Table 2-3.

[0049] Table 2 Indoor activity test results of fluopyram and fluopyram activated ester complex against rice blast fungus

[0050]

[0051] From the results in Table 2, it can be seen that when the mass ratio of thiamethoxam and fluazifop is 1-40:10-1, they have a synergistic effect on rice blast fungus; especially when the ratio is between 1-5:3-1, the co-toxicity coefficients of the listed ratios are all higher than 147.2866, and the synergistic effect is more obvious.

[0052] Table 3 Indoor activity test results of fluopyram and magnolol against rice blast fungus

[0053]

[0054]

[0055] From the results in Table 3, it can be seen that when the mass ratio of thiamethoxam and fluazifop is 1-20:20-1, they have a synergistic effect on rice blast fungus; especially when the ratio is between 10:1, the co-toxicity coefficient is as high as 6, and the synergistic effect is more obvious.

[0056] As can be seen from the above, the combination of thiamethoxam and fluazifop or magnolol of the present invention shows a synergistic effect. Compared with a single agent, the pesticide composition of the present invention can improve the control effect of plant diseases, reduce the amount of pesticide used in the field, and effectively reduce environmental pollution and pesticide residues.

Claims

1. A pesticide composition containing thiamethoxam, characterized in that: The pesticide composition is prepared by compounding thiamethoxam and activated thiamethoxam or magnolol, and the mass ratio of thiamethoxam to activated thiamethoxam or magnolol is 1-500:500-1.

2. The pesticide composition according to claim 1, characterized in that The mass ratio of the thiazide to the fluazifop-butyl activated ester is 1-40:10-1.

3. The pesticide composition according to claim 2, characterized in that The mass ratio of thiazide to fluazifop-butyl is 1-5:3-1.

4. The pesticide composition according to claim 1, characterized in that The mass ratio of thiazide to magnolol is 1-20:20-1.

5. The pesticide composition according to claim 1, characterized in that The mass ratio of thiazide to magnolol is 10:1.

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