Odor-removing antibacterial emulsion containing compound plant essential oil for vehicles and preparation method of odor-removing antibacterial emulsion

Abstract

The invention belongs to the technical field of natural bacteriostatic agents, and particularly relates to an odor-removing bacteriostatic emulsion containing compound plant essential oil for vehicles and a preparation method of the odor-removing bacteriostatic emulsion. The antibacterial emulsion is prepared from, by mass, 1%-2% of rhizoma atractylodis essential oil, 1%-5% of ligusticum wallichii essential oil, 1%-3% of elsholtzia essential oil, 1%-5% of pericarpium citri reticulatae essential oil, 1%-5% of star anise essential oil, 20%-30% of ligusticum wallichii aromatic water and 20%-30% of elsholtzia aromatic water, 4%-10% of an emulsifier and 10%-20% of a dispersing agent are added into the components, and the uniform antibacterial emulsion is prepared. The volatility of the essential oil is utilized, so that the essential oil is freely volatilized in a vehicle, bacteria are inhibited, peculiar smell is adsorbed, and a green and fresh air environment is provided for the interior of the vehicle.

Description

Technical Field

[0001] The invention belongs to the technical field of natural antibacterial agents, and in particular relates to a vehicle-use deodorizing antibacterial emulsion containing compound plant essential oil and a preparation method thereof. Background Art

[0002] With the development of the times, the number of cars is increasing, and the closed environment inside the car provides conditions for the growth of bacteria, especially Staphylococcus aureus. And as time accumulates, the odor and harmful gases emitted by the interior of the car will also have adverse effects on the human body. At present, most car interior cleaning and disinfection use chemical agents, which may cause secondary pollution when cleaning the car, bringing a bad experience to the people in the car who are in this environment for a long time.

[0003] Natural plants have a long history of medicinal use in China, and many essential oil components have also been clinically proven to have antibacterial activity. They are popular because of their low toxicity, greenness, and environmentally friendly preparation. Some plant essential oils can also absorb foreign matter and harmful gases, playing a role in refreshing the air.

[0004] However, the essential oils currently on the market have also exposed some drawbacks in practical applications. For example, due to the high volatility of pure essential oil products, the antibacterial effect of essential oils is unstable or even weak during use; for another example, the low water solubility and complex composition of essential oils themselves lead to poor compatibility of compound essential oils, making it difficult to prepare suitable dosage forms. Summary of the invention

[0005] The purpose of the present invention is to solve the current problems of bacterial contamination and odor in automobiles, and to provide a deodorizing and antibacterial emulsion containing compound plant essential oil for automobiles and an application method thereof.

[0006] In order to achieve the above-mentioned purpose, the present invention provides a deodorizing and antibacterial emulsion for vehicles containing compound plant essential oils, and the raw material composition thereof is calculated by weight percentage: 1%-2% of atractylodes lancea essential oil, 1%-5% of chuanxiong essential oil, 1%-3% of elsholtzia essential oil, 1%-5% of tangerine peel essential oil, 1%-5% of star anise essential oil, 20%-30% of chuanxiong aromatic water, 20%-30% of elsholtzia aromatic water, 4%-10% of emulsifier, and 10%-20% of dispersant.

[0007] Among them, the extraction methods of atractylodes lancea essential oil, tangerine peel essential oil and star anise essential oil are as follows: crush atractylodes lancea, tangerine peel and star anise respectively, put them in a round-bottom flask, add deionized water, pretreat them by ultrasound for 10 minutes, heat them to boiling, extract volatile oil by steam distillation, collect the upper oily liquid in the volatile oil extractor, and obtain the essential oil.

[0008] The extraction methods of Chuanxiong essential oil, Elsholtzia essential oil, Chuanxiong aromatic water and Elsholtzia aromatic water are as follows: respectively crush Chuanxiong and Elsholtzia, put them in a round-bottom flask, add deionized water, pre-treat them by ultrasonic for 10 minutes, heat them to boiling, extract them by steam distillation, collect the upper oily liquid in the volatile oil extractor to obtain the essential oil, and collect the water liquid in the lower layer of the volatile oil extractor to obtain the aromatic water.

[0009] The mass volume ratio of solid to liquid in the above extraction process is 1:8 to 1:12; and the extraction time is 2 to 3.5 hours.

[0010] The emulsifier is Tween 80 and the dispersant is olive oil.

[0011] The preparation method of the deodorizing and antibacterial emulsion containing compound plant essential oil for vehicles is as follows:

[0012] (1) according to the above mass percentages, respectively, add atractylodes essential oil, chuanxiong essential oil, elsholtzia essential oil, tangerine peel essential oil, star anise essential oil and emulsifier Tween 80 to the dispersant olive oil to obtain an oil phase after the oil is completely dispersed;

[0013] (2) taking a mixture of the Rhizoma Chuanxiong and the Rhizoma Elsholtziae aromatic water according to the above mass percentages as the aqueous phase;

[0014] (3) At a water bath temperature of 56° C. and a stirring speed of 400 rpm, the oil phase is added to the water phase while stirring, so that the oil phase and the water phase are evenly mixed to obtain a uniform plant essential oil emulsion.

[0015] Beneficial Effects

[0016] The emulsion of the present invention is applied in the car, and its volatility and stability must be taken into consideration. Therefore, different natural plant essential oils are reasonably combined to prepare a compound emulsion, and the volatility of the essential oils is utilized to allow the essential oils to evaporate freely in the car, inhibiting bacteria while absorbing odors, and providing a fresh air environment in the car while maintaining the advantages of greenness and safety.

[0017] In the preparation process of the emulsion, the aromatic water produced in the essential oil extraction process is selected as the aqueous phase of the emulsion, thereby reducing the instability of the composite essential oil; in addition, in view of the high volatility of the essential oil, the present invention designs an application form of the compound essential oil emulsion, which, while utilizing the volatility of the essential oil, also enables the effective ingredients in the essential oil to achieve a slow release effect, thereby avoiding the unstable efficacy caused by its high volatility. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is the result of the inhibition zone of the emulsion against Staphylococcus aureus in Example 1.

[0019] Figure 2This is the result diagram of the inhibition zone of the emulsion against Staphylococcus aureus when the extraction time of each essential oil is 3 hours in Example 3.

[0020] Figure 3 This is the result diagram of the inhibition zone of the emulsion against Staphylococcus aureus in Example 5.

[0021] Figure 4 This is the result diagram of the inhibition zone of the emulsion against Staphylococcus aureus in Comparative Example 1.

[0022] Figure 5 This is the result diagram of the inhibition zone of the emulsion against Staphylococcus aureus in Comparative Example 2.

[0023] Figure 6 This is the result diagram of the inhibition zone of the emulsion against Staphylococcus aureus in Comparative Example 3.

[0024] Figure 7 Total ion current chromatogram of Atractylodes lancea essential oil detected by gas chromatography-mass spectrometry.

[0025] Figure 8 Total ion current chromatogram of Chuanxiong essential oil detected by gas chromatography-mass spectrometry. DETAILED DESCRIPTION

[0026] The present invention will be further described below in conjunction with embodiments, but is not limited thereto.

[0027] Example 1

[0028] 1. Extraction of various plant essential oils and aromatic waters

[0029] (1) 100 g each of Atractylodes lancea, dried tangerine peel, and star anise were respectively ground into coarse powder, passed through a 20-mesh sieve, placed in a round-bottom flask, and deionized water with a solid-to-liquid ratio of 1:12 (W / V) was added. The mixture was ultrasonically pretreated at 30° C. for 10 minutes. The round-bottom flask was removed, and a volatile oil extractor was placed on top of the flask, heated to boiling, and steam distillation was used to extract volatile oil from Atractylodes lancea, volatile oil from dried tangerine peel, and volatile oil from star anise. After extraction for 2.5 hours, the oily liquid in the volatile oil extractor was collected to obtain Atractylodes lancea essential oil, volatile oil from dried tangerine peel, and essential oil from star anise, respectively.

[0030] (2) 100 g each of Chuanxiong and Elsholtzia ciliata were respectively ground into coarse powder, passed through a 20-mesh sieve, placed in a round-bottom flask, and deionized water with a solid-liquid ratio of 1:12 (W / V) was added. The mixture was ultrasonically pretreated at 30°C for 10 min. The round-bottom flask was removed, and a volatile oil extractor was placed on top of it. The mixture was heated to boiling, and steam distillation was used to extract the volatile oil of Atractylodes lancea and the volatile oil of Elsholtzia ciliata. After extraction for 2.5 hours, the upper layer of the oily liquid in the volatile oil extractor was collected to obtain Chuanxiong essential oil and Elsholtzia ciliata essential oil, respectively. The water in the middle and lower layers of the volatile oil extractor was collected to obtain Chuanxiong aromatic water and Elsholtzia ciliata aromatic water, respectively.

[0031] 2. Preparation method of plant essential oil emulsion

[0032] Atractylodes lancea essential oil 1%, Chuanxiong essential oil 5%, Elsholtzia essential oil 3%, Tangerine peel essential oil 5%, Star anise essential oil 5%, Chuanxiong aromatic water 30%, Elsholtzia aromatic water 25%, Tween 80 emulsifier 8%, olive oil 18%.

[0033] (1) According to the above mass percentages, atractylodes essential oil, chuanxiong essential oil, elsholtzia essential oil, tangerine peel essential oil, star anise essential oil and emulsifier Tween 80 are added to olive oil to completely disperse them to obtain the oil phase of the emulsion.

[0034] (2) According to the above mass percentage, take the aromatic water of Chuanxiong and the aromatic water of Elsholtzia ciliata and mix them evenly to obtain the aqueous phase of the emulsion.

[0035] (3) adding the oil phase to the water phase while stirring at a water bath temperature of 56° C. and a stirring speed of 400 rpm to uniformly mix the oil phase and the water phase to obtain a uniform plant essential oil emulsion.

[0036] Example 2

[0037] The preparation method of this embodiment is the same as that of Example 1, except that when the steam distillation method is used to extract the Atractylodes lancea essential oil, the solid-liquid ratio of the solvent used is different. The volume of the Atractylodes lancea essential oil obtained under different extraction conditions is shown in Table 1.

[0038] Table 1

[0039] Different extraction solvent solid-liquid ratio (W / V) Essential oil volume (mL) Solid-liquid ratio 1:7 1.43 Solid-liquid ratio 1:8 1.51 Solid-liquid ratio 1:9 1.62 Solid-liquid ratio 1:10 1.66 Solid-liquid ratio 1:11 1.73 Solid-liquid ratio 1:12 1.91 Solid-liquid ratio 1:13 1.97 Solid-liquid ratio 1:14 1.98

[0040] As can be seen from Table 1, when different solid-liquid ratios of solvents are used to extract essential oils, the solid-liquid ratios are different, and the volumes of essential oils extracted are also different. When the solid-liquid ratio (W / V) is 1:7, the volume of the obtained Atractylodes lancea essential oil is 1.43mL. As the amount of extraction solvent increases, the volume of essential oil extracted also increases continuously. When the solid-liquid ratio (W / V) is 1:12, the volume of Atractylodes lancea essential oil reaches 1.91mL. When the amount of solvent is further increased and the solid-liquid ratio (W / V) ratio of 1:13 and 1:14 is used for extraction, the volumes of essential oil obtained are 1.97mL and 1.98mL, respectively. It can be seen that when the volume of the extraction solvent exceeds 12 times the amount of medicinal materials, the amount of Atractylodes lancea essential oil obtained does not increase much, but the increase in the amount of solvent will bring a series of cumbersome operations. Therefore, the present invention optimizes the solid-liquid ratio (W / V) of the solvent used in the extraction of Atractylodes lancea essential oil to 1:12. Similarly, the optimized ratio of solvent solid-liquid ratio (W / V) used in the extraction of other essential oils was also set at 1:12.

[0041] Example 3

[0042] The preparation method of this embodiment is the same as that of Example 1, except that when the steam distillation method is used to extract the Atractylodes lancea essential oil, the extraction time used is different. The specific extraction conditions and the volumes of the Atractylodes lancea essential oil obtained are shown in Table 2.

[0043] Table 2

[0044]

[0045] As can be seen from Table 2, with the increase of extraction time, the volume of essential oil extracted also increases continuously, and when the extraction time is 3h, the volume of Atractylodes lancea essential oil reaches 1.92mL. And continue to increase the extraction time, when reaching 3.5h, the volume of Atractylodes lancea essential oil obtained is respectively 2.03mL. It can be seen that when the extraction time exceeds 3h, the amount of Atractylodes lancea essential oil increases little, considering that the extraction time is longer and has a certain influence on the stability of volatile oil components, and the time cost of extraction, the present invention optimizes the Atractylodes lancea essential oil extraction time to 3h. Similarly, several other essential oil extraction times are also optimized to 3h.

[0046] Example 4

[0047] The preparation method of this embodiment is the same as that of embodiment 1, except that in the second step of the preparation method of the plant essential oil emulsion, the raw materials are composed of:

[0048] Atractylodes lancea essential oil 1%, Chuanxiong essential oil 1%, Elsholtzia essential oil 3%, Tangerine peel essential oil 3%, Star anise essential oil 3%, Chuanxiong aromatic water 30%, Elsholtzia aromatic water 30%, Tween 80 emulsifier 10%, olive oil 19%.

[0049] Example 5

[0050] The preparation method of this embodiment is the same as that of embodiment 1, except that in the second step of the preparation method of the plant essential oil emulsion, the raw materials are composed of:

[0051] Atractylodes lancea essential oil 1%, Chuanxiong essential oil 5%, Elsholtzia essential oil 3%, Tangerine peel essential oil 5%, Star anise essential oil 5%, Chuanxiong aromatic water 30%, Elsholtzia aromatic water 27%, Tween 80 emulsifier 4%, olive oil 20%.

[0052] Comparative Example 1

[0053] The preparation method of this embodiment is the same as that of embodiment 1, except that in the first step of the plant essential oil extraction method, the medicinal raw materials are not subjected to ultrasonic pretreatment, but are directly extracted by steam distillation.

[0054] Comparative Example 2

[0055] The preparation method of this embodiment is the same as that of embodiment 1, except that in the second step of the preparation method of the plant essential oil emulsion, distilled water is used instead of Chuanxiong aromatic water and Elsholtzia aromatic water, that is, the raw material composition is:

[0056] Atractylodes lancea essential oil 1%, Ligusticum chuanxiong essential oil 5%, Elsholtzia ciliata essential oil 3%, Tangerine peel essential oil 5%, Star anise essential oil 5%, distilled water 55%, Tween 80 emulsifier 8%, olive oil 18%.

[0057] Comparative Example 3

[0058] The preparation method of this embodiment is the same as that of embodiment 1, except that this embodiment does not contain plant essential oil extracts, but only contains Chuanxiong aromatic water and Elsholtzia aromatic water, that is, the raw material composition is: 30% Chuanxiong aromatic water, 25% Elsholtzia aromatic water, 8% Tween 80 emulsifier, and 37% olive oil.

[0059] Comparative Example 4

[0060] The preparation method is the same as that in Example 1, except that this example does not contain Atractylodes lancea essential oil and Tangerine peel essential oil extract, that is, the raw material composition is: 5% Chuanxiong essential oil, 3% Elsholtzia essential oil, 5% Star Anise essential oil, 30% Chuanxiong aromatic water, 30% Elsholtzia aromatic water, 8% Tween 80 emulsifier, and 19% olive oil.

[0061] Antibacterial experiment: inhibition zone experiment

[0062] The experiment was conducted with reference to the standards of the Clinical Laboratory Standards Institute (CLSI). 15 mL of melted nutrient agar medium was added to a sterile plate and allowed to solidify. 10 mL of nutrient agar medium was taken up with a pipette. 6 Place 100uL of CFU / mL Staphylococcus aureus suspension on the plate and spread it evenly with a coating rod. Take another drug-sensitive paper (6mm in diameter), soak it in the sample solution to be tested for 30 minutes, take it out, and air-dry it in a sterile environment. Stick the drug-sensitive paper of the test sample and the blank control group (sterile water) on the plate, with the control sample in the middle and the test sample on the periphery. Incubate in a 37℃ incubator for 24 hours and observe the formation of the inhibition zone on the plate.

[0063] The sensitivity judgment standard of antibacterial effect: the diameter of the antibacterial circle <10mm is judged as low sensitivity and ineffective "-"; 10mm≤antibacterial diameter <15mm is judged as medium sensitivity and effective "+"; 15mm≤antibacterial diameter <20mm is judged as high sensitivity and effective "++". Each test sample must be repeated three times. If there is an inhibitory effect in all three experiments and no antibacterial circle is produced in the negative control group, the experimental result is judged to be qualified.

[0064] The antibacterial effects of Examples 1-5 and Comparative Examples 1-4 (emulsions) on Staphylococcus aureus are shown in Table 3 and Attached Figure 1-6 shown.

[0065] Table 3

[0066] Diameter of inhibition zone (mm) Result determination Example 1 18.01 ++ Example 2 (solvent solid-liquid ratio 1:13) 17.82 ++ Example 3 (essential oil extraction time is 3h) 17.98 ++ Example 4 13.04 + Example 5 15.13 ++ Comparative Example 1 14.22 + Comparative Example 2 13.74 + Comparative Example 3 6.25 - Comparative Example 4 11.35 +

[0067] Analysis experiment of volatile components of Atractylodes lancea essential oil and Ligusticum chuanxiong essential oil:

[0068] GC-MS (gas chromatography-mass spectrometry) was used to detect the volatile components of the essential oils of Atractylodes lancea and Ligusticum chuanxiong.

[0069] Chromatographic conditions: instrument model Agilent 7890 / 5975C, chromatographic column: HP-5MS (30m×250μm×0.25μ), diluent chromatographic grade dichloromethane; temperature program: from column temperature 100°C for 0 min, increase to 220°C at a heating rate of 3°C / min, and maintain for 5 min; carrier gas is high-purity He; column flow rate is 1.2 ml / min, split ratio: 50:1.

[0070] Mass spectrometry conditions: EI source; ion source temperature 230°C; MS quadrupole temperature 150°C; injection port temperature 220°C; scanning range 30-550 amu.

[0071] The total ion current chromatogram of Atractylodes lancea essential oil is attached. Figure 7 , among which the content of β-eucalyptol is relatively high, reaching 39.85%; the total ion flow chromatogram of Chuanxiong essential oil is attached Figure 8 , among which the content of ligustilide is relatively high, reaching 66.93%.

[0072] Investigation on improvement of air freshness in vehicles:

[0073] Table 4

[0074]

[0075] Select cars with similar air conditions in the car, take 20mL of each of the product embodiments 1-5 and the control examples 1-4 (emulsions) of the present invention, apply them in a closed car, let them evaporate freely, and invite 11 people to observe and experience the fresh air in the car 2 hours and 12 hours after use. In addition, select a blank group without any product as a blank control. The improvement of the air freshness in the car by each group of samples is shown in Table 4. As can be seen from the table, the product embodiments 1-5 of the present invention have a certain improvement on the air condition in the car after 2 hours of use, and there is no irritation as time goes by.

Claims

1. A deodorizing and antibacterial emulsion containing compound plant essential oil for use in vehicles, characterized in that: The antibacterial emulsion is composed of the following components by weight: 1%-2% of atractylodes essential oil, 1%-5% of chuanxiong essential oil, 1%-3% of elsholtzia essential oil, 1%-5% of tangerine peel essential oil, 1%-5% of star anise essential oil, 20%-30% of chuanxiong aromatic water, 20%-30% of elsholtzia aromatic water, 4%-10% of emulsifier and 10%-20% of dispersant.

2. The deodorizing and antibacterial emulsion containing compound plant essential oil for use in a vehicle as claimed in claim 1, characterized in that: The extraction method of the atractylodes lancea essential oil, the tangerine peel essential oil and the star anise essential oil comprises the following steps: crushing the atractylodes lancea, the tangerine peel and the star anise respectively, placing the crushed atractylodes lancea, the tangerine peel and the star anise in a round-bottom flask, adding deionized water, performing ultrasonic pretreatment for 10 minutes, heating the mixture to boiling, extracting volatile oil by steam distillation, and collecting the upper oily liquid in the volatile oil extractor to obtain the essential oil.

3. The deodorizing and antibacterial emulsion containing compound plant essential oil for vehicle use according to claim 1, characterized in that: The extraction methods of the Chuanxiong essential oil, the Elsholtzia essential oil, the Chuanxiong aromatic water and the Elsholtzia aromatic water are as follows: respectively crushing Chuanxiong and the Elsholtzia in a round-bottom flask, adding deionized water, pre-treating them by ultrasonic for 10 minutes, heating them to boiling, extracting them by steam distillation, collecting the upper oily liquid in the volatile oil extractor to obtain the essential oil, and collecting the water liquid in the lower layer of the volatile oil extractor to obtain the aromatic water.

4. The deodorizing and antibacterial emulsion containing compound plant essential oil for vehicle use as claimed in claim 2 or 3, characterized in that: The solid-liquid mass volume ratio during the extraction process is 1:8 to 1:12; the extraction time is 2 to 3.5 hours.

5. The deodorizing and antibacterial emulsion containing compound plant essential oil for vehicle use according to claim 1, characterized in that: The emulsifier is Tween 80 and the dispersant is olive oil.

6. A method for preparing the deodorizing and antibacterial emulsion containing compound plant essential oil for vehicles as claimed in claim 1, characterized in that: The preparation method comprises the following steps: according to the mass percentage, adding atractylodes lancea essential oil, chuanxiong essential oil, elsholtzia essential oil, tangerine peel essential oil, star anise essential oil and an emulsifier into a dispersant to completely disperse the essential oils to obtain an oil phase; according to the mass percentage, taking chuanxiong aromatic water and elsholtzia aromatic water and mixing them as a water phase; and then adding the oil phase into the water phase while stirring to uniformly mix the oil phase and the water phase to obtain an antibacterial emulsion containing the compound plant essential oil.

7. The method for preparing the deodorizing and antibacterial emulsion containing compound plant essential oil for vehicles as claimed in claim 6, characterized in that: When the oil phase was added to the water phase, the water bath temperature was 56°C and the stirring speed was 400 rpm.

8. An application of the deodorizing and antibacterial emulsion containing compound plant essential oil for vehicles as claimed in claim 1, characterized in that: The antibacterial emulsion is used as an emulsion for removing odor in a car, refreshing the air, and inhibiting Staphylococcus aureus.

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