Method for extracting celery seed essential oil by using deep-eutectic solvent as well as product and application of celery seed essential oil

Abstract

The invention discloses a method for extracting celery seed essential oil by using a deep-eutectic solvent as well as a product and application of the celery seed essential oil. The method comprises the following steps: firstly, crushing and sieving celery seeds to obtain celery seed powder; mixing the celery seed powder and the eutectic solvent according to the mass ratio of 1: 4, and reacting for 60 minutes at the temperature of 50-70 DEG C; and then carrying out steam distillation, collecting volatile oil obtained by distillation, and drying to obtain the celery seed essential oil. The celery seed essential oil is extracted by adopting the eutectic solvent, the cost is low, and the operation is convenient. The celery seed essential oil extracted by the method disclosed by the invention has relatively strong capability of removing DPPH anion free radicals, has good oxidation resistance, and can be widely applied to the fields of seasonings, medicines, cosmetics and the like.

Description

Technical Field

[0001] The present invention belongs to the technical field of essential oil processing, and specifically relates to a method for extracting celery seed essential oil using a low eutectic solvent, and a product and application thereof. Technical Background

[0002] Celery has a long history of cultivation in my country and is widely distributed. Celery contains carotene, vitamins, a variety of flavonoids and volatile aromatic components, and has excellent medicinal functions. Its fruit, celery seeds, also contain volatile substances and are widely used as food additives. For example, celery seeds are added to foods such as soy sauce and meat. Celery seed extracts have been used to treat dysmenorrhea and joint pain. In addition to its edible and medicinal value, celery seeds can also be used to extract essential oils and oleoresins. According to research reports, celery seed essential oil has good antioxidant, anti-tumor, antibacterial and insecticidal biological activities.

[0003] A low eutectic solvent refers to a two-component or three-component low eutectic mixture composed of a certain stoichiometric ratio of hydrogen bond acceptors (such as quaternary ammonium salts) and hydrogen bond donors (such as compounds such as amides, carboxylic acids and polyols), and its freezing point is significantly lower than the melting point of the pure substances of each component. The raw materials of low eutectic solvents are inexpensive, simple to prepare, safe and non-toxic. Using low eutectic solvents to extract celery seed essential oil will improve the extraction efficiency of celery seed essential oil, optimize the extraction method of celery seed essential oil, help to increase the added value of celery seeds, and greatly promote the utilization of my country's spice resources. Summary of the invention

[0004] The invention aims to provide a method for extracting celery seed essential oil by using a deep eutectic solvent.

[0005] The object of the present invention is achieved by the following scheme: A method for extracting celery seed essential oil with a deep eutectic solvent comprises the following steps: (1) pulverizing celery seeds, sieving into celery seed powder of 50-80 mesh, and drying for later use; (2) preparing a low eutectic solvent, mixing the hydrogen bond acceptor and the hydrogen bond donor in a constant temperature water bath at 50° C. to 70° C., and heating and stirring for 60 min to form a uniform and clear low eutectic solvent; (3) mixing the low eutectic solvent with the celery seed powder, performing steam distillation, collecting and drying the volatile oil obtained by the distillation, and obtaining the celery seed essential oil.

[0006] The celery seeds in step (1) are firstly crushed, sieved and dried, and the particle size of the celery seeds is 80 meshes.

[0007] In step (2), the hydrogen bond donor is 1,3-propylene glycol, and the hydrogen bond acceptor is choline chloride.

[0008] The molar ratio of the hydrogen bond donor to the hydrogen bond acceptor is 2:1.

[0009] In step (3), the mass ratio of celery seed powder to low eutectic solvent is 1:4.

[0010] The distillation extraction conditions in step (3) are to maintain the temperature between 50 and 70° C. and perform the distillation extraction for 60 minutes.

[0011] The invention provides a celery seed essential oil, which is characterized by being prepared according to any one of the above methods.

[0012] The invention provides application of celery seed essential oil in preparing antioxidant essential oil.

[0013] The low eutectic solvent used in the present invention is low in cost and easy to obtain, and the extraction technology used is simple, easy to learn, and easy to operate. In general, the present invention has the advantages of low cost and easy operation. The celery seed essential oil extracted by the present invention has a strong scavenging ability for DPPH anion free radicals, has good antioxidant properties, and can be widely used in the fields of condiments, medicines, cosmetics, etc. DETAILED DESCRIPTION

[0014] The present invention is implemented by the following specific embodiments.

[0015] Example 1 A deep eutectic solvent is used to extract celery seed essential oil, and the preparation method is as follows: (1) Crush the dried celery seeds with a grinder, pass through an 80-mesh sieve to obtain celery seed powder, and transfer to a dryer for storage; (2) Add choline chloride and 1,3-propylene glycol in a ratio of 1:2 into a round-bottom flask, mix, heat and stir in a constant temperature water bath at 60°C for 60 minutes to form a uniform and clear low eutectic solvent; (3) Mix celery seed powder and a low eutectic solvent in a mass ratio of 1:4, add the mixture into a round-bottom flask, and stir in a constant temperature water bath at 60°C for 10 min to obtain a mixed solution; (4) Add 200 ml of deionized water to the mixed solution of step (3) and perform steam distillation in a distillation apparatus for 1 hour. Collect the volatile oil obtained by distillation and dry it with anhydrous sodium sulfate before storing it for later use.

[0016] Comparative Example 1 The conventional distillation extraction method extracts essential oils and is prepared according to the following steps: (1) Crush the dried celery seeds with a grinder, pass through an 80-mesh sieve to obtain celery seed powder, and transfer to a dryer for storage; (2) Add celery seed powder to 200 ml of deionized water and perform steam distillation in a distillation apparatus. Perform steam distillation extraction for 1 hour. Collect the volatile oil obtained by distillation and dry it with anhydrous sodium sulfate before storing it for later use.

[0017] Calculate the essential oil yield of Example 1 and Comparative Example 1: Attached Table 1 is the essential oil yield obtained after calculation.

[0018] The free radical scavenging rate of Example 1 and Comparative Example 1 was determined by the DPPH method. The DPPH free radical scavenging ability of the celery seed essential oil obtained in Example 1 and Comparative Example 1 was measured by the DPPH method to determine the antioxidant capacity of the essential oil. Appendix 2 is the antioxidant capacity of the essential oil obtained in Example 1 and Comparative Example 1.

[0019] This experiment uses the Jining Industrial Total Antioxidant Capacity (T-AOC) kit, and the specific steps are as follows: (1) The control group took 20 μl of the extract and added 380 μl of reagent 1. The experimental group took 20 μl of the essential oil solution of Example 1 and the essential oil solution of Comparative Example 1 and added 380 μl of reagent 1.

[0020] (2) Mix thoroughly and incubate at room temperature in the dark for 20 min.

[0021] (3) Take 100 μl of the control group and experimental group samples and add them to a 96-well plate. Detect the absorbance of the samples at 515 nm on an ELISA reader. 517 .

[0022] DPPH free radical scavenging rate % = (OD 空白 -OD 测定 ) / OD 空白 ×100% Table 1 shows the yields of essential oils extracted from Example 1 and Comparative Example 1.

[0023] As shown in Table 1, the yield of essential oil from celery seeds by using a low eutectic solvent is higher than that by the traditional method: As can be seen from Table 1, the yield of essential oil from celery seeds using a low eutectic solvent is higher than that of the traditional method.

[0024] Table 2 reflects the DPPH free radical scavenging rate of the essential oils extracted from Example 1 and Comparative Example 1: As shown in Table 2, the DPPH radical scavenging rate of celery seed essential oil extracted by low eutectic solvent is similar to that of celery seed essential oil extracted by traditional distillation method, indicating that celery seed essential oil extracted by low eutectic solvent has better antioxidant effect.

Claims

1. A method for extracting celery seed essential oil using a deep eutectic solvent, characterized in that: The following steps are included: Crush the celery seeds, sieve them into 50-80 mesh celery seed powder, and dry them for later use; A low eutectic solvent is prepared by mixing a hydrogen bond acceptor and a hydrogen bond donor in a constant temperature water bath at 50° C. to 70° C., and heating and stirring for 60 minutes, wherein the molar ratio of the hydrogen bond donor to the hydrogen bond acceptor is 2:1, to form a uniform and clear low eutectic solvent; The low eutectic solvent is mixed with celery seed powder, and steam distillation is performed. The volatile oil obtained by the distillation is collected and dried to obtain celery seed essential oil.

2. The method for extracting celery seed essential oil with a deep eutectic solvent according to claim 1, characterized in that: The celery seeds are firstly crushed, sieved and dried, and the particle size of the celery seeds is 80 meshes.

3. The method for extracting celery seed essential oil with a deep eutectic solvent according to claim 1, characterized in that: In the preparation of the low eutectic solvent, the hydrogen bond donor is 1,3-propylene glycol and the hydrogen bond acceptor is choline chloride.

4. The method for extracting celery seed essential oil with a deep eutectic solvent according to claim 1, characterized in that: The mass ratio of celery seed powder to deep eutectic solvent is 1:

4.

5. The method for extracting celery seed essential oil using a deep eutectic solvent according to claim 1, characterized in that: The conditions for distillation extraction are to maintain the temperature between 50-70°C and distill for 60 minutes.

6. The method for extracting celery seed essential oil using a deep eutectic solvent according to any one of claims 1 to 5, characterized in that: Prepare as follows: (1) Crush the dried celery seeds with a grinder, pass through an 80-mesh sieve to obtain celery seed powder, and transfer to a dryer for storage; (2) Add choline chloride and 1,3-propylene glycol in a ratio of 1:2 into a round-bottom flask, mix, heat and stir in a constant temperature water bath at 60°C for 60 minutes to form a uniform and clear low eutectic solvent; (3) Mix celery seed powder and a low eutectic solvent in a mass ratio of 1:4, add the mixture into a round-bottom flask, and stir in a constant temperature water bath at 60°C for 10 min to obtain a mixed solution; (4) Add 200 ml of deionized water to the mixed solution of step (3) and perform steam distillation in a distillation apparatus for 1 hour. Collect the volatile oil obtained by distillation and dry it with anhydrous sodium sulfate before storing it for later use.

7. A celery seed essential oil, characterized in that Prepared according to any one of claims 1 to 6.

8. Use of the celery seed essential oil according to claim 7 in the preparation of antioxidant essential oil.

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