Probiotic culture equipment and method convenient for sampling

Abstract

The invention discloses a probiotic culture device and method convenient for sampling, and the device comprises a box body which has the functions of constant temperature and sterilization; a rotating module, a driving mechanism, a fixed seat, a rotating bracket and a self-rotating and lifting integrated mechanism are arranged in the box body, the fixed seat is arranged at the box bottom, the rotating bracket is arranged above the fixed seat, the self-rotating and lifting integrated mechanism comprises a rotating supporting rod and a multi-cavity sleeve, the sleeve is provided with a circular inner cavity matched with the supporting rod, and the driving mechanism drives the sleeve to rotate; the lifting sampling mechanism comprises a telescopic device and a driving unit which are arranged on a fixed seat, a tray is arranged at the telescopic end, a rotary bracket is provided with an annular seat for supporting the culture dish, the tray is telescopic below the annular seat, and a sampling chamber at the upper end of the box body is communicated with the interior and directly faces the tray. Compared with the prior art, a rotating mechanism is matched with a lifting mechanism, a top cover does not need to be opened, a single culture dish can be sent to a sampling chamber for independent sampling, safety is guaranteed, infectious microbe pollution is avoided, sampling is convenient, and the sampling problem of multi-layer multi-dish culture equipment is solved.

Description

Technical Field

[0001] The invention relates to the field of probiotic culture for feed in animal husbandry technology, and in particular to a probiotic culture device and method which is convenient for sampling. Background Art

[0002] Probiotics are a type of active microorganisms that are beneficial to the host (such as animals or humans). They mainly include bacteria (such as lactic acid bacteria, bifidobacteria, bacillus, etc.) and yeast. These microorganisms can colonize in the host's intestines and other parts, and have a positive impact on the host's health and physiological functions by improving the balance of intestinal microorganisms in the host. Adding probiotics to feed can improve animal growth performance, improve feed palatability, maintain animal intestinal health, regulate the balance of intestinal flora, enhance intestinal mucosal barrier function, and improve animal immunity.

[0003] Probiotics need to be cultivated in a special cultivation device. Most of the existing probiotic inoculation and cultivation devices are box-type structures. For example, the prior art of CN 118460344A discloses a probiotic cultivation device and a culture box for animal feeding, which cultivates probiotics by arranging a self-rotating component inside the box to drive the cultivation device to rotate; and the prior art of CN213232275U discloses a probiotic inoculation and cultivation device, which cultivates probiotics by arranging a multi-layer culture rack in the box to place culture dishes. However, the growth of probiotics is a dynamic process, and its growth rate, production of metabolites, etc. will vary greatly at different stages. If real-time sampling cannot be performed, these key information cannot be obtained in time. The lack of real-time sampling will make it difficult for operators to adjust the culture conditions in time. However, many culture equipment are not designed with the convenience of real-time sampling in mind, including but not limited to the above-mentioned multi-culture dish inoculation and cultivation type probiotic culture equipment. There are many inconveniences when sampling during the culture process. For example, some complex multi-layer structures and closed culture systems are difficult to perform real-time sampling without interfering with the normal growth of probiotics. Since there are many independent culture dishes in the constant temperature box, when sampling some or all of these culture dishes, it is necessary to open the box door and sample a single culture dish in a targeted manner. In addition, due to the small space of the multi-layer and multi-culture dish culture equipment, it is inconvenient to operate, and it is extremely easy to cause large-scale bacterial contamination during the sampling process. Summary of the invention

[0004] In order to solve the problems that the multi-culture dish type probiotic culture equipment in the prior art is difficult to operate during sampling and is prone to causing large-scale bacterial infection, the present invention proposes a probiotic culture equipment and method that is easy to sample.

[0005] The technical solution adopted by the invention is: a probiotic culture device that is easy to sample, comprising: a box body, the box body has a constant temperature and sterilization function; a rotating module, the rotating module is arranged in the box body, the rotating module comprises a driving mechanism, a fixed seat, a rotating bracket and a self-rotating and lifting integrated mechanism, the fixed seat is arranged at the bottom of the box body, the rotating bracket is arranged above the fixed seat, the self-rotating and lifting integrated mechanism comprises a rotating support rod and a multi-cavity sleeve, the rotating support rod is arranged in the middle of the fixed seat, the middle of the rotating bracket is provided with a multi-cavity sleeve, the multi-cavity sleeve comprises a circular inner cavity arranged along its length direction, the circular inner cavity and the rotating The movable support rod is adapted, and the driving mechanism is used to drive the multi-chamber sleeve to rotate on the rotating support rod; a lifting sampling mechanism, the lifting sampling mechanism includes a telescopic device and a driving unit arranged on the fixed seat, the telescopic end of the telescopic device is provided with a tray, the rotating bracket includes a plurality of annular seats arranged on the circumference of the multi-chamber sleeve, the annular seat is used to support the culture dish, the tray is telescopically arranged below the annular seat, and a sampling chamber is provided at the upper end of the box body, the sampling chamber is connected with the box body and is opposite to the tray, and any culture dish is lifted and raised to the sampling chamber by the telescopic movement of the tray for manual or automatic sampling.

[0006] Furthermore, the multi-chamber sleeve also includes an annular outer cavity arranged along its length direction on the outside of the circular inner cavity, and a first thread is provided on the circumferential surface of one side of the annular outer cavity, and the self-rotation and lifting integrated mechanism also includes a threaded sleeve, which is arranged in the annular outer cavity and threadedly connected with the first thread of the annular outer cavity, and the threaded sleeve includes a rotating end on the outside of the lower end of the multi-chamber sleeve, and a one-way bearing is provided between the rotating end and the rotating support rod. When the driving mechanism drives the multi-chamber sleeve to rotate in a direction same as the rotation direction of the threaded sleeve, and the rotation direction of the multi-chamber sleeve is consistent with the rotation direction allowed by the one-way bearing, so as to drive the rotating bracket to rotate, when the driving mechanism drives the multi-chamber sleeve to rotate in a direction opposite to the rotation direction of the threaded sleeve, so as to drive the rotating bracket to rise on the rotating support rod.

[0007] Furthermore, the driving mechanism includes a driving motor arranged on the rotating support rod, the output end of the driving motor is provided with a driving gear, the driving gear is arranged in the circular inner cavity, and the inner wall of the circular inner cavity is provided with a circumferentially arranged rack, and the driving gear is meshed with the rack.

[0008] Furthermore, the box body comprises a bottom box and a top cover, the side wall of the bottom box is provided with an interlayer, a coil is provided in the interlayer, and the coil is respectively provided with a liquid inlet and a liquid outlet for circulating and transporting a constant temperature liquid.

[0009] Furthermore, the top cover is provided with the sampling chamber, the lower end of the sampling chamber is provided with a switch door, and the sampling chamber is also provided with a steam joint for conveying high-temperature steam for disinfection.

[0010] Furthermore, a driving unit is provided on the top cover, and a slide is provided in the sampling chamber, the switch door is slidably arranged in the slide, and the switch door is drivingly connected to the driving unit.

[0011] Furthermore, a high-temperature steam inlet is provided at the lower end of the bottom box, and a plurality of through holes are provided in the fixing seat.

[0012] Furthermore, the telescopic device is a scissor-fork mechanism, the pallet is arranged at the top end of the scissor-fork mechanism, and the lower end of the pallet is provided with a first fixed-point rotating end and a first slide groove, and the upper end of the scissor-fork mechanism is respectively connected to the first fixed-point rotating end and the first slide groove; the upper end of the fixed seat is provided with a first groove, the first groove is used to place the pallet, and the bottom of the first groove is also provided with a second groove, the inner side of the second groove is provided with a second fixed-point rotating end and a second slide groove, and the lower end of the scissor-fork mechanism is respectively connected to the second fixed-point rotating end and the second slide groove; the driving unit includes a screw and a driving motor, one end of the screw is drivingly connected to the driving motor, and a nut is also provided on the sliding connection side of the lower end of the scissor-fork mechanism and the second slide groove, the nut is threadedly connected to the screw, and the screw rotates to drive the telescopic and extension of the scissor-fork mechanism.

[0013] Furthermore, the present invention proposes a sampling method for a probiotic culture device, including the following methods: single sampling, disinfecting the sampling chamber, rotating the rotating bracket to rotate one of the annular seats and the culture dish to the corresponding upper side of the tray, driving the telescopic device to rise and lift the corresponding culture dish to the sampling chamber for sampling; collective sampling, opening the top cover of the box, driving the rotating bracket to rise on the rotating support rod, and lifting all the culture dishes to the top of the box for sampling or collection.

[0014] Compared with the prior art, the present invention, through the cooperation between the rotating module and the lifting sampling mechanism, takes a single culture dish into the sampling chamber for individual sampling without opening the top cover, thereby ensuring the safety of sampling and effectively avoiding the possible contamination of bacteria caused by the contact of the remaining culture dishes with the air. In addition, sampling is convenient and fast, effectively overcoming the problem of inconvenient operation of multi-layer and multi-culture dish culture equipment during sampling. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0016] Figure 1 It is a schematic diagram of the three-dimensional structure of the probiotics culture device of the present invention; Figure 2 It is a side structural schematic diagram of the box part of the probiotic culture device of the present invention; Figure 3 It is a schematic cross-sectional structure diagram of the box body of the probiotics culture device of the present invention; Figure 4 It is a schematic diagram of the three-dimensional structure of the rotating module in the present invention; Figure 5 It is a schematic diagram of the explosion structure of the rotating module in the present invention; Figure 6 It is a schematic diagram of the cross-sectional exploded structure of the rotating module in the present invention; Figure 7 It is a schematic diagram of the cross-sectional assembly structure of the rotating module in the present invention; Figure 8 It is a partial cross-sectional schematic diagram of the lifting sampling mechanism in the present invention; Fig. 9 The figure is a schematic diagram showing the three-dimensional structure of the telescopic device in the present invention.

[0017] The main figures in this application are: 1. Box body; 11. Bottom box; 12. Top cover; 13. Interlayer; 14. Liquid inlet; 15. Liquid outlet; 16. Sampling chamber; 17. Open / close door; 18. Steam joint; 19. High-temperature steam pipe; 2. Rotating module; 21. Driving mechanism; 22. Fixed seat; 23. Rotating bracket; 24. Self-rotating lifting integrated mechanism; 241. Rotating support rod; 242. Multi-cavity sleeve; 243. Circular inner cavity; 25. Annular seat; 26. Culture dish; 244. Annular outer cavity; 245. First thread ; 27. Threaded sleeve; 271. Rotating end; 246. Shoulder rest; 28. One-way bearing; 211. Drive motor; 212. Drive gear; 213. Rack; 3. Lifting sampling mechanism; 31. Telescopic device; 32. Tray; 33. Small suction cup; 34. First fixed-point rotating end; 35. First slide groove; 36. First groove; 37. Second groove; 38. Second fixed-point rotating end; 39. Second slide groove; 321. Screw; 322. Drive motor; 323. Adapter rod; 324. Nut. DETAILED DESCRIPTION

[0018] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0019] In the following description, with reference to the accompanying drawings, several embodiments of the present application are described. It should be understood that other embodiments may also be used, and mechanical composition, structure, electrical and operational changes may be made without departing from the spirit and scope of the present disclosure. The following detailed description should not be considered restrictive, and the scope of the embodiments of the present application is limited only by the claims of the published patents. The terms used here are only to describe specific embodiments and are not intended to limit the present application. Spatially related terms, such as "upper", "lower", "left", "right", "below", "below", "lower", "above", "upper", etc., may be used in the text to facilitate the description of the relationship between an element or feature shown in the figure and another element or feature.

[0020] The invention discloses a probiotics culture device that is convenient for sampling. Figures 1 to 3 As shown, the probiotic culture device in the present invention mainly includes three parts: a box body 1, a rotating module 2 and a lifting sampling mechanism 3. The box body 1 is composed of a bottom box 11 and a top cover 12. A high-temperature steam connection pipe 19 is provided at the bottom of the bottom box 11. The top cover 12 can be set by flipping or other forms of switches. An interlayer 13 is provided on the side wall of the bottom box 11. A coil is provided in the interlayer 13. The coil is respectively provided with a liquid inlet 14 and a liquid outlet 15 for circulating and transporting a constant temperature liquid. The temperature inside the box body 1 is controlled by passing a constant temperature medium into the coil. A sampling chamber 16 is provided on the top cover 12. The sampling chamber 16 and the top cover 12 are connected to each other. It is integrally formed, and a partition is formed between the bottom of the sampling chamber 16 and the inside of the top cover 12 by a switch door 17. The switch door 17 is closed and opened by pulling or flipping. The technical means of the switch door 17 can adopt any conventional setting in the prior art. A steam joint 18 specially used for disinfection can be set inside the sampling chamber 16. The bacteria inside the sampling chamber 16 are disinfected by high-temperature steam to ensure that the probiotics in the culture dish 26 will not be contaminated during the sampling process. In addition to the introduction of high-temperature steam, the bacteria inside the sampling chamber 16 can also be disinfected by setting an ultraviolet lamp inside the sampling chamber 16.

[0021] Further, in an practicable manner, for example, a driving device is provided on the top cover 12, and the sampling chamber 16 is provided with a slideway, the switch door 17 is slidably provided in the slideway, the switch door 17 is transmission-connected with the driving device, and the driving device is, for example, a telescopic cylinder, which pushes the switch door 17 to slide at the bottom of the sampling chamber 16 to realize the opening and closing of the sampling chamber 16. This scheme is only an example of an practicable embodiment, and other schemes of the switchable door 17 in the prior art are applicable to the present invention, which will not be described in detail here.

[0022] Furthermore, combined with Figures 4 to 7 As shown, the rotating module 2 is arranged in the box body 1, and the rotating module 2 includes a driving mechanism 21, a fixed seat 22, a rotating bracket 23 and a self-rotating lifting integrated mechanism 24. The fixed seat 22 is arranged at the bottom of the box body 1, and the rotating bracket 23 is arranged above the fixed seat 22. The self-rotating lifting integrated mechanism 24 includes a rotating support rod 241 and a multi-cavity sleeve 242. The rotating support rod 241 is arranged in the middle of the fixed seat 22, and the middle of the rotating bracket 23 is provided with a multi-cavity sleeve 242. The multi-cavity sleeve 242 includes a circular inner cavity 243 arranged along its length direction, and the circular inner cavity 243 and the rotating support The support rod 241 is adapted, and the driving mechanism 21 is used to drive the multi-chamber sleeve 242 to rotate on the rotating support rod 241; there are several annular seats 25 on the circumferential side of the multi-chamber sleeve 242 of the rotating bracket 23, and the annular seat 25 is used to support the culture dish 26. The bottom of the culture dish 26 passes through the annular seat 25, and the inner circle of the annular seat 25 fixes the culture dish 26 in a bracket manner. During the culture process, the driving mechanism 21 drives the rotating bracket 23 to rotate with the rotating support rod 241 as the axis, and the bacteria inside the culture dish 26 are dispersed by rotation, which is beneficial to the normal growth of the bacteria.

[0023] Moreover, the multi-chamber sleeve 242 also includes an annular outer chamber 244, which is arranged outside the circular inner chamber 243 along the length direction of the multi-chamber sleeve 242, and both the annular outer chamber 244 and the circular inner chamber 243 are provided with an opening at the bottom of the multi-chamber sleeve 242, and a first thread 245 is provided on the circumferential surface of one side of the annular outer chamber 244, and the self-rotating and lifting integrated mechanism 24 also includes a threaded sleeve 27, which is arranged in the annular outer chamber 244 and is threadedly connected to the first thread 245 of the annular outer chamber 244, and the lower end of the threaded sleeve 27 is a rotating end 271, and the rotating end 271 is located outside the lower end of the multi-chamber sleeve 242. When installed, the rotating end 271 is opposite to the rotating support rod 241, and the bottom of the rotating support rod 241 is provided with a shoulder support 246, and the rotating end 2 A one-way bearing 28 is provided between 71 and the shoulder support 246 of the rotating support rod 241, wherein the thread rotation direction of the multi-cavity sleeve 242 is consistent with the rotation direction allowed by the one-way bearing 28. At this time, when rotating in this direction, the multi-cavity sleeve 242 and the threaded sleeve 27 are in a tightened state, and the threaded sleeve 27 and the rotating bracket 23 will rotate synchronously; and when the driving mechanism 21 drives the multi-cavity sleeve 242 to rotate in a direction opposite to the rotation direction of the threaded sleeve 27, the threaded sleeve 27 cannot rotate under the action of the one-way bearing 28, but the multi-cavity sleeve 242 rotates in the reverse direction. At this time, the multi-cavity sleeve 242 and the threaded sleeve 27 rotate, and the multi-cavity sleeve 242 drives the rotating bracket 23 to rise. By driving the multi-cavity sleeve 242 to rotate forward and reverse, the normal rotation and overall lifting of the rotating bracket 23 can be realized.

[0024] Furthermore, combined with Figure 6 , 7 As shown, the driving mechanism 21 includes a driving motor 211 arranged on a rotating support rod 241, and a driving gear 212 is provided at the output end of the driving motor 211. The driving gear 212 is arranged in a circular inner cavity 243. The power cord of the driving motor 211 is built into the rotating support rod 241 and is connected to the outside, and a circumferentially arranged rack 213 is provided on the inner wall of the circular inner cavity 243, and the length direction of the rack 213 is vertically arranged. The driving gear 212 is meshed with the rack 213, and the rotation and lifting of the rotating bracket 23 are realized by the forward and reverse rotation of the driving motor 211. This structure embeds the driving mechanism 21 in the circular inner cavity 243. The space is relatively closed, which can effectively extend the service life of the driving mechanism 21, and ensure that the interference of the mechanism is reduced as much as possible, so as to realize the refinement and simplification of the overall equipment.

[0025] Furthermore, combined with Figure 8 , 9As shown, the lifting sampling mechanism 3 includes a telescopic device 31 and a driving unit. The telescopic device 31 and the driving unit are arranged on the fixed seat 22. A tray 32 is provided at the telescopic end of the telescopic device 31. The tray 32 is provided with a plurality of small suction cups 33 made of silicone. The tray 32 is telescopically arranged below the annular seat 25. The sampling chamber 16 is connected to the box body 1 and is opposite to the tray 32. By telescoping the tray 32, any culture dish 26 can be lifted and raised to the sampling chamber 16 for manual or automatic sampling.

[0026] Furthermore, the telescopic device 31 adopts a scissor-type mechanism, which has high foldability and stability, can save internal space well, and compress and store the scissor-type mechanism; the tray 32 is arranged at the top end of the scissor-type mechanism, and the lower end of the tray 32 is provided with a first fixed-point rotating end 34 and a first slide groove 35 (the first fixed-point rotating end 34 and the first slide groove 35 are arranged in pairs), and the top ends of the two cross-hinged rods above the scissor-type mechanism are respectively hinged to the first fixed-point rotating end 34, and are slidably connected with the first slide groove 35. In addition, a first groove 36 is provided at the upper end of the fixing seat 22, and the size of the first groove 36 is consistent with the tray 32, so that the tray 32 can be placed and stored when the scissor mechanism is retracted. A second groove 37 is provided at the bottom of the first groove 36, and a second fixed-point rotating end 38 and a second slide groove 39 (arranged in pairs) are provided inside the second groove 37. The lower ends of the bottoms of the two cross hinged rods below the scissor mechanism are respectively hingedly connected to the second fixed-point rotating end 38 and slidably connected to the second slide groove 39; the driving unit includes a screw 321 And the driving motor 322, one end of the screw 321 is connected to the driving motor 322, the lower end of the scissor mechanism is connected to the second slide groove 39 sliding connection side through a transfer rod 323 and the screw 321, a nut 324 is provided on the transfer rod 323, the nut 324 and the screw 321 are threadedly connected, when the screw 321 rotates, the scissor mechanism can be synchronously driven to telescope and lift, and the culture dish 26 is lifted by the tray 32 to synchronously rise, and when descending, the annular seat 25 and the culture dish 26 can be abutted to return to their original position, and the scissor mechanism can continue to descend.

[0027] The working principle and method of sampling of the present invention are as follows: when sampling a single culture dish 26, after the sampling chamber 16 is disinfected, ensure that the sampling chamber 16 is clean and free of pollution, and control the driving mechanism 21 to rotate to a certain angle through the PLC, so that the culture dish 26 to be sampled is located directly above the tray 32, and then control the lifting sampling mechanism 3 to rise (synchronously open the switch door 17 of the sampling chamber 16) to lift the culture dish 26 into the sampling chamber 16, and then perform sampling work (manual sampling or automatic sampling), and after sampling, control the lifting sampling mechanism 3 to descend (synchronously close the switch door 17 of the sampling chamber 16) to return the culture dish 26 downward to the annular seat 25. When collective sampling or probiotics collection is performed, open the top cover 12 of the box 1, drive the rotating bracket 23 to rise on the rotating support rod 241, and lift all the culture dishes 26 above the box 1 for collective sampling or probiotics collection. The present invention, through the cooperation between the rotating module 2 and the lifting sampling mechanism 3, takes a single culture dish 26 into the sampling chamber 16 for individual sampling without opening the top cover 12, thereby ensuring the safety of sampling and effectively avoiding the possible contamination of bacteria caused by the contact of the remaining culture dishes 26 with the air. In addition, sampling is convenient and fast, effectively overcoming the problem of inconvenient operation of multi-layer and multi-culture dish 26 culture equipment during sampling.

[0028] It should be noted that: in the present invention, when manual sampling is performed in the sampling chamber 16, a switchable door needs to be opened on the sampling chamber 16, and the operator manually reaches into the sampling chamber 16 to perform sampling work; when an automatic sampling device is used, an automatic sampling device can be configured in the sampling chamber 16 to collect samples. This is a prior art, for example, the automatic sampling device proposed in 202222149989.5 is used, so the present invention will not repeat it.

[0029] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A probiotic culture device that is convenient for sampling, characterized in that: include: A box (1), wherein the box (1) has a constant temperature and sterilization function; A rotating module (2), wherein the rotating module (2) is arranged in the housing (1), the rotating module (2) comprises a driving mechanism (21), a fixed seat (22), a rotating bracket (23) and a self-rotating lifting integrated mechanism (24), the fixed seat (22) being arranged at the bottom of the housing (1), the rotating bracket (23) being arranged above the fixed seat (22), the self-rotating lifting integrated mechanism (24) comprising a rotating support rod (241) and a multi-cavity sleeve (242), the rotating support rod (241) being arranged at the middle of the fixed seat (22), the multi-cavity sleeve (242) being arranged at the middle of the rotating bracket (23), the multi-cavity sleeve (242) comprising a circular inner cavity (243) arranged along its length direction, the circular inner cavity (243) being adapted to the rotating support rod (241), and the driving mechanism (21) being used for driving the multi-cavity sleeve (242) to rotate on the rotating support rod (241); A lifting sampling mechanism (3), the lifting sampling mechanism (3) comprising a telescopic device (31) and a driving unit arranged on the fixed seat (22), the telescopic end of the telescopic device (31) being provided with a tray (32), the rotating bracket (23) comprising a plurality of annular seats (25) arranged on the circumference of the multi-cavity sleeve (242), the annular seats (25) being used to support a culture dish (26), the tray (32) being telescopically arranged below the annular seats (25), and a sampling chamber (16) being provided at the upper end of the box body (1), the sampling chamber (16) being communicated with the inside of the box body (1) and facing the tray (32), and any culture dish (26) being lifted and raised to the sampling chamber (16) by the telescopic movement of the tray (32) for manual or automatic sampling.

2. The probiotics culture device according to claim 1, characterized in that: The multi-cavity sleeve (242) further comprises an annular outer cavity (244) arranged outside the circular inner cavity (243) along its length direction, and a first thread (245) is provided on a circumferential surface of one side of the annular outer cavity (244). The self-rotating lifting integrated mechanism (24) further comprises a threaded sleeve (27), the threaded sleeve (27) being arranged in the annular outer cavity (244) and being threadedly connected to the first thread (245) of the annular outer cavity (244). The threaded sleeve (27) comprises a rotating end (271) outside the lower end of the multi-cavity sleeve (242), and the rotating end (271) is provided on the outer side of the lower end of the multi-cavity sleeve (242). A one-way bearing (28) is provided between the rotating support rod (241) and the rotating support rod (241). When the driving mechanism (21) drives the multi-cavity sleeve (242) to rotate in the same direction as the rotation direction of the threaded sleeve (27), and the rotation direction of the multi-cavity sleeve (242) is consistent with the rotation direction allowed by the one-way bearing (28), the rotating support rod (241) is driven to rotate. When the driving mechanism (21) drives the multi-cavity sleeve (242) to rotate in the opposite direction to the rotation direction of the threaded sleeve (27), the rotating support rod (23) is driven to rise on the rotating support rod (241).

3. The probiotics culture device according to claim 2, characterized in that: The driving mechanism (21) comprises a driving motor (211) arranged on the rotating support rod (241); a driving gear (212) is provided at the output end of the driving motor (211); the driving gear (212) is provided in the circular inner cavity (243); and a circumferentially arranged rack is provided on the inner wall of the circular inner cavity (243); the driving gear (212) meshes with the rack.

4. The probiotics culture device according to claim 1, characterized in that: The box body (1) comprises a bottom box (11) and a top cover (12); a side wall of the bottom box (11) is provided with an interlayer (13); a coil is provided in the interlayer (13); and the coil is provided with a liquid inlet (14) and a liquid outlet (15) for circulating and transporting a constant temperature liquid.

5. The probiotics culture device according to claim 4, characterized in that: The top cover (12) is provided with the sampling chamber (16), the lower end of the sampling chamber (16) is provided with a switch door (17), and the sampling chamber (16) is also provided with a steam connector (18) for conveying high-temperature steam for disinfection.

6. The probiotics culture device according to claim 5, characterized in that: The top cover (12) is provided with a driving unit, and the sampling chamber (16) is provided with a slideway, the switch door (17) is slidably arranged in the slideway, and the switch door (17) is drivingly connected to the driving unit.

7. The probiotics culture device according to claim 5, characterized in that: A high-temperature steam inlet is also provided at the lower end of the bottom box (11), and a plurality of through holes are provided on the fixing seat (22).

8. The probiotics culture device according to claim 1, characterized in that: The telescopic device (31) is a scissor-fork mechanism, the tray (32) is arranged at the top end of the scissor-fork mechanism, and the lower end of the tray (32) is provided with a first fixed-point rotating end (34) and a first slide groove (35), and the upper end of the scissor-fork mechanism is respectively connected to the first fixed-point rotating end (34) and the first slide groove (35); the upper end of the fixed seat (22) is provided with a first groove (36), the first groove (36) is used to place the tray (32), and the bottom of the first groove (36) is further provided with a second groove (37), and the inner side of the second groove (37) is provided with a second groove (37). The scissor mechanism comprises two fixed-point rotating ends (38) and a second slide groove (39), and the lower end of the scissor mechanism is respectively connected to the second fixed-point rotating end (38) and the second slide groove (39); the driving unit comprises a screw rod (321) and a driving motor (322), one end of the screw rod (321) is drivingly connected to the driving motor (322), and a nut (324) is further provided on the sliding connection side between the lower end of the scissor mechanism and the second slide groove (39), and the nut (324) is threadedly connected to the screw rod (321), and the screw rod (321) rotates to drive the extension and retraction of the scissor mechanism.

9. A sampling method for a probiotic culture device, characterized in that: The following methods are included: Single sampling, disinfecting the sampling chamber (16), rotating the rotating bracket (23) so that one of the annular seats (25) and the culture dish (26) rotates to the top of the corresponding tray (32), and driving the telescopic device (31) to rise to lift the corresponding culture dish (26) to the sampling chamber (16) for sampling; Collective sampling is performed by opening the top cover (12) of the box body (1), driving the rotating bracket (23) to rise on the rotating support rod (241), and lifting all the culture dishes (26) to the top of the box body (1) for sampling or collection.

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