Stem cell culture device

Abstract

The invention discloses a stem cell culture device. The device comprises an incubator; glass is arranged on four walls of the incubator, and a sealing cover is arranged at the top of the incubator; the culture container is arranged in the culture box; the movement mechanism is arranged in the incubator; the analysis mechanism is arranged in the incubator; the calibration mechanism is arranged on the movement mechanism; the infusion mechanism is arranged on the incubator; and the controller is arranged at the top of the incubator. A culture container in the incubator is moved through the movement mechanism, the liquid level in the culture container is judged through the analysis mechanism, the culture container is further positioned through the calibration mechanism, and the infusion mechanism further judges whether the culture container is below the infusion mechanism or not through a photoelectric sensor. Finally, the culture medium in the culture container is added through the liquid conveying mechanism.

Description

Technical Field

[0001] The invention relates to the field of biotechnology, in particular to a stem cell culture device. Background Art

[0002] Stem cell culture is a key technology in the biomedical field and is of great significance to disease treatment, tissue engineering, and drug development.

[0003] Stem cells are a type of cell with unlimited or immortal self-renewal ability, and can produce at least one type of highly differentiated daughter cells. In the cell culture process, since cells such as stem cells need to be expanded and cultured, it is necessary to add cell culture medium into the cell culture container. However, although frequent manual operations can achieve the desired effect, injecting cell culture medium into a large number of cell culture containers often wastes a lot of staff's energy and reduces the staff's effective working time.

[0004] During cell culture, many stem cells have the characteristic of adhering to the wall for growth. During the adherent culture process, stem cells will adhere to the wall of the culture container (such as culture flasks, culture dishes, and test tubes, etc.). Existing culture containers can only provide limited space for stem cells to adhere to the wall for growth, which restricts the growth space of stem cells during stem cell culture and has an adverse effect on the cultivation of stem cells.

[0005] Therefore, the present invention proposes a stem cell culture device to solve the above problems and bring better user experience.

[0006] Invention patent content

[0007] The object of the present invention is to provide a stem cell culture device to solve the problems raised in the above background technology.

[0008] In order to solve the above technical problems, the present invention provides the following technical solution: a stem cell culture device, comprising:

[0009] Incubator; the four walls of the incubator are provided with glass, and the top is provided with a sealing cover;

[0010] A culture container is arranged inside the incubator and serves as a place for cultivating stem cells;

[0011] The motion mechanism is arranged inside the incubator to drive the culture container to move in the incubator;

[0012] An analysis mechanism is disposed inside the incubator to monitor the culture medium in the culture container;

[0013] A calibration mechanism, disposed on the motion mechanism, for calibrating the stop position of the motion mechanism;

[0014] An infusion mechanism is provided on the incubator to add culture medium into the culture container;

[0015] The controller is arranged on the top of the incubator, receives signals from the infusion mechanism, the calibration mechanism, the analysis mechanism and the motion mechanism, and completes the overall regulation of the above mechanisms according to the received signals; a touch screen is arranged on the top of the controller.

[0016] Preferably, the culture container includes a test tube, which is arranged inside the moving mechanism; a scale line, which is arranged on the outside of the test tube; a cell culture plate, which is arranged inside the test tube; a groove, which is arranged at the center of the cell culture plate; a cover plate, which is arranged on the top of the test tube; a feed port, which is arranged on the cover plate and is concentric with the groove; and an embedded groove, which is arranged on the cover plate and corresponds to the cell culture plate.

[0017] Preferably, the motion mechanism includes a motor, which is arranged at the bottom of the outer side of the incubator; a rotating base, which is arranged at the bottom of the inner side of the incubator and is connected to the driving end of the motor; a gear, which is arranged on the side of the motor drive shaft and is located at the bottom of the outer side of the incubator; a support rod, which is arranged on the rotating base and is located at the center of the rotating base; a culture rack, which is arranged above the rotating base and is connected to the support rod; a fixing hole, which is arranged on the culture rack; and a circular pit, which is arranged on the rotating base and is concentric with the fixing hole.

[0018] Preferably, the analysis mechanism includes a camera, which is arranged inside the incubator; a lighting lamp, which is arranged inside the incubator; and signal output ends of the camera and the lighting lamp are connected to the signal input end of the controller.

[0019] Preferably, the calibration mechanism includes a rotation limit switch, which is arranged at the bottom outside the incubator and connected to the gear; a Hall sensor, which is arranged on the incubator rack; a small power supply, which is arranged on the Hall sensor; and the signal output ends of the rotation limit switch and the Hall sensor are connected to the signal input end of the controller.

[0020] Preferably, the infusion mechanism includes a liquid storage tank, which is arranged on the top of the incubator; an infusion tube, which is arranged inside the incubator and connected to the liquid storage tank; an injection pump, which is arranged above the liquid storage tank; a photoelectric sensor, which is arranged on the top of the liquid storage tank; and a liquid level sensor, which is arranged inside the liquid storage tank; and the signal output ends of the photoelectric sensor, the liquid level sensor and the injection pump are connected to the signal input end of the controller.

[0021] Preferably, the method for using the stem cell culture device comprises the following steps:

[0022] Step A: The analysis mechanism takes a picture of the scale line height at which the culture medium liquid level in the culture container is located, and the obtained picture is transmitted to the controller for identification;

[0023] Step B: The controller controls the motion mechanism to drive the culture container to move according to the information transmitted by the analysis mechanism;

[0024] Step C: the calibration mechanism sends a signal to the controller, and the controller controls the motion mechanism to stop working; the controller further calibrates the position of the culture container according to the detection result of the calibration mechanism;

[0025] Step D: After the calibration is completed, the controller controls the touch screen to control the infusion mechanism to complete the addition of culture medium inside the culture container according to the culture medium injection volume set by the staff.

[0026] Preferably, the step C comprises:

[0027] Step C1, the rotation limit switch sends a signal to the controller, and the controller controls the motor to stop working according to the signal sent by the rotation limit switch;

[0028] Step C2, the Hall sensor detects the current position of the culture rack and sends an adjustment signal to the controller;

[0029] Step C3, the controller controls the motor to calibrate the position of the culture rack according to the adjustment signal sent by the Hall sensor.

[0030] Preferably, the step D comprises:

[0031] Step D1, the photoelectric sensor determines that the test tube is below and sends a signal to the controller that the culture medium can be injected;

[0032] Step D2, after receiving the signal from the photoelectric sensor, the controller controls the injection pump to start working according to the culture medium injection amount set by the staff;

[0033] In step D3, the liquid level sensor transmits information on the amount of culture medium injected set by the staff to the controller, and the controller stops the injection work of the injection pump according to the information transmitted by the liquid level sensor to complete the injection of the culture medium.

[0034] Compared with the prior art, the present invention has the following beneficial effects:

[0035] 1. The present invention moves the culture container in the incubator through a motion mechanism, uses an analysis mechanism to determine the height of the liquid level in the culture container to obtain the amount of culture medium that should be injected, further positions the culture container through a calibration mechanism, and the infusion mechanism further determines whether the culture container is below the infusion mechanism through the photoelectric sensor, and finally completes the addition of culture medium inside the culture container through the infusion mechanism.

[0036] 2. The present invention uses a high-definition camera, so the operator can easily observe the culture status and discover and deal with problems in a timely manner.

[0037] 3. The present invention effectively reduces the residual and oxidation of the culture solution through the built-in liquid storage tank and the infusion tube, and the infusion mechanism avoids the interaction between the internal and external environments caused by frequent opening of the incubator during infusion. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 It is a schematic diagram of the overall internal structure of an embodiment of the present invention;

[0039] Figure 2 It is a schematic top view of the overall structure of an embodiment of the present invention;

[0040] Figure 3 This is a schematic diagram of the internal structure after the cover is removed according to an embodiment of the present invention;

[0041] Figure 4 A schematic diagram of the structure of a culture container according to an embodiment of the present invention;

[0042] Figure 5 It is a schematic diagram of the front view structure of a culture container according to an embodiment of the present invention;

[0043] Figure 6 Schematic diagram of the internal structure of a culture container according to an embodiment of the present invention.

[0044] In the figure: 100, incubator; 110, glass; 120, cover; 200, culture container; 210, test tube; 220, scale mark; 230, cell culture plate; 240, groove; 250, cover; 260, feed port; 270, embedding groove; 300, motion mechanism; 310, rotating base; 320, gear; 330, support rod; 340, culture rack; 350, fixing hole; 360, round pit; 370, motor; 400, analysis mechanism; 410, camera; 420, lighting; 500, calibration mechanism; 510, rotation limit switch; 520, Hall sensor; 530, small power supply; 600, infusion mechanism; 610, photoelectric sensor; 620, liquid level sensor; 630, injection pump; 640, liquid storage tank; 650, infusion tube; 700, controller; 710, touch screen. DETAILED DESCRIPTION

[0045] In order to solve the problems described in the background technology, the embodiment of the present invention provides a stem cell culture device. The technical scheme in the embodiment of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiment of the present invention. Obviously, the described embodiment is only a part of the embodiment of the present invention, not all of the embodiments. Based on the embodiment of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0046] See also Figure 1-6 This embodiment provides a stem cell culture device and a method for using the same, including:

[0047] Incubator 100; the four walls of the incubator 100 are provided with glass 110, and the top is provided with a cover 120. The glass 110 is provided around to facilitate the staff to observe the culture container 200 from the side;

[0048] The culture container 200 is disposed inside the incubator 100 and serves as a place for cultivating stem cells;

[0049] The motion mechanism 300 is disposed inside the incubator 100 and drives the culture container 200 to move in the incubator 100;

[0050] The analysis mechanism 400 is disposed inside the incubator 100 and monitors the culture medium in the culture container 200;

[0051] The calibration mechanism 500 is disposed on the motion mechanism 300 and calibrates the stop position of the motion mechanism 300;

[0052] The infusion mechanism 600 is disposed on the incubator 100 and adds the culture medium into the culture container 200;

[0053] The controller 700 is arranged on the top of the incubator 100, receives signals from the infusion mechanism 600, the calibration mechanism 500, the analysis mechanism 400 and the movement mechanism 300, and completes the overall control of the above mechanisms according to the received signals; a touch screen 710 is arranged on the top of the controller 700, and the staff can operate on the touch screen 710 to set the amount of culture medium injected into the culture container 200, and can also shoot through the camera 410 and transmit the picture to the touch screen 710 for observation.

[0054] Specifically, the culture container 200 includes a test tube 210, which is arranged inside the movement mechanism 300, with the bottom placed in the round pit 360 and passing through the fixing hole 350 on the culture rack 340; the scale line 220 is arranged on the outside of the test tube 210 to help the staff determine whether the culture medium in the test tube 210 needs to be supplemented; the cell culture plate 230 is arranged inside the test tube 210, and the cell culture plate 230 is two vertically crossed plates to increase the clinging space of stem cells; one end of the cell culture plate 230 located at the top of the test tube 210 extends out of the test tube 210 and is connected to the embedded groove 270 on the cover plate 250; the groove 240 is arranged at the center of the cell culture plate 230, when the culture medium is injected into the test tube 210 from the feed port 260, the groove 240 can play a buffering effect, preventing the injected culture medium from directly contacting the culture medium in the test tube 210, thereby generating splashing of the culture medium; the cover plate 250 is arranged on the top of the test tube 210 to prevent the movement mechanism 3 00 drives the test tube 210 to move, and the culture medium splashes out of the test tube 210; the feed port 260 is arranged on the cover plate 250, and is concentric with the groove 240, and the culture medium is injected from here; the embedding groove 270 is arranged on the cover plate 250, and corresponds to the cell culture plate 230; the corresponding meaning is that the depth of the embedding groove 270 is equal to the extension of the cell culture plate 230 from the test tube 210, the shape and thickness of the embedding groove 270 are the same as the cross shape and thickness of the cell culture plate 230, and the cell The portion of the culture plate 230 extending out of the test tube 210 should not be too long and should be smaller than the thickness of the cover 250 to prevent the cell culture plate 230 from penetrating the cover 250; the cover 250 and the embedding groove 270 provided on the cover 250 play a role in fixing the cell culture plate 230 to prevent the cell culture plate 230 from shaking in the test tube 210 when the movement mechanism 300 drives the culture container 200 to move, thereby affecting the adhesion state of the stem cells and causing adverse effects on the cultivation of the stem cells.

[0055] Specifically, the motion mechanism 300 includes a motor 370, which is arranged at the bottom of the outer side of the incubator 100 to drive the rotating base 310 and the support rod 330 to rotate; the rotating base 310 is arranged at the bottom of the inner side of the incubator 100 and is connected to the driving end of the motor 370; the gear 320 is arranged on the side of the transmission shaft of the motor 370, located at the bottom of the outer side of the incubator 100, and assists the rotation limit switch 510 to judge the rotation of the transmission shaft of the motor 370; the support rod 330 is arranged on the rotating base 310, located at the center of the rotating base 310, and plays a supporting role for the culture rack 340; the culture rack 340 is arranged above the rotating base 310 and is connected to the support rod 330; the fixing hole 350 is arranged on the culture rack 340; the circular pit 360 is arranged on the rotating base 310 and is concentric with the fixing hole 350.

[0056] Specifically, the analysis mechanism 400 includes a camera 410, which is arranged inside the incubator 100. Several cameras 410 shoot the test tube 210 from different angles, making it convenient for the staff to observe the test tube 210 from different angles through the touch screen 710; an illumination lamp 420 is arranged inside the incubator 100 to provide brightness for the shooting of the camera 410; the signal output ends of the camera 410 and the illumination lamp 420 are connected to the signal input end of the controller 700.

[0057] Specifically, the calibration mechanism 500 includes a rotation limit switch 510, which is arranged at the bottom of the outer side of the incubator 100 and connected to the gear 320. The rotation of the gear 320 sends information to the controller 700 to help the controller 700 control the start and stop of the motor 370; the Hall sensor 520 is arranged on the incubator rack 340 and can detect the magnetic field changes of the rotating base 310. By comparing the continuously read Hall sensor 520 signals, the position deviation of the rotating base 310 is detected; a small power supply 530 is arranged on the Hall sensor 520 to provide power for the Hall sensor 520; the signal output ends of the rotation limit switch 510 and the Hall sensor 520 are connected to the signal input end of the controller 700.

[0058] Specifically, the infusion mechanism 600 includes a liquid storage tank 640, which is arranged on the top of the incubator 100; an infusion tube 650, which is arranged inside the incubator 100 and connected to the liquid storage tank 640; an injection pump 630, which is arranged above the liquid storage tank 640; a photoelectric sensor 610, which is arranged on the liquid storage tank 640, and determines whether there is a culture container 200 below by the returned distance value. If there is a culture rack 340 below, the reflected signal is fast and the distance is short; otherwise, if the signal is slow and the distance is far, it is a culture container 200; a liquid level sensor, which is arranged inside the liquid storage tank 640, detects the height of the liquid level drop inside the liquid storage tank 640, and determines the amount of culture medium injected; the signal output ends of the photoelectric sensor 610, the liquid level sensor, and the injection pump 630 are connected to the signal input end of the controller 700.

[0059] Specifically, the method for using the stem cell culture device comprises the following steps:

[0060] Step A: The analysis mechanism 400 takes a picture of the height of the scale line 220 where the culture medium liquid level in the culture container 200 is located, and the obtained picture is transmitted to the controller 700 for recognition. The staff sets the culture medium injection amount according to the height of the culture medium liquid level in the picture taken by the camera 410;

[0061] Step B: the controller 700 controls the motion mechanism 300 to drive the culture container 200 to move according to the information transmitted by the analysis mechanism 400;

[0062] Step C: the calibration mechanism 500 sends a signal to the controller 700, and the controller 700 controls the motion mechanism 300 to stop working; the controller 700 further calibrates the position of the culture container 200 according to the detection result of the calibration mechanism 500;

[0063] Step D: After the calibration is completed, the controller 700 controls the touch screen 710 to control the infusion mechanism 600 to complete the addition of the culture medium inside the culture container 200 according to the culture medium injection amount set by the staff.

[0064] Specifically, the step C comprises:

[0065] Step C1, when the rotation speed of the motor 370 reaches the maximum speed at which it needs to stop, the rotation limit switch 510 sends a signal to the controller 700, and the controller 700 controls the motor 370 to stop working according to the signal sent by the rotation limit switch 510. When the motor 370 stops working, the rotating base 310 continues to rotate by inertia to reach an approximate injection position;

[0066] Step C2, the Hall sensor 520 detects the current position of the culture rack 340 through the change of the magnetic field of the culture rack 340, determines the distance that the culture rack 340 needs to move, and sends an adjustment signal to the controller 700;

[0067] In step C3, the controller 700 controls the motor 370 to work according to the adjustment signal sent by the Hall sensor 520, and the motor 370 calibrates the position of the culture rack 340.

[0068] Specifically, the step D comprises:

[0069] Step D1, the photoelectric sensor 610 determines that the test tube 210 is below and sends a signal to the controller 700 that the culture medium can be injected. If the test tube 210 is not below, the photoelectric sensor 610 sends a signal to the controller 700. After receiving the signal, the controller 700 controls the motor 370 for further calibration.

[0070] Step D2, after receiving the signal from the photoelectric sensor 610, the controller 700 controls the injection pump 630 to start working and inject the culture medium into the test tube 210 according to the culture medium injection amount set by the staff;

[0071] In step D3, the liquid level sensor 620 transmits the information of the medium injection amount set by the staff to the controller 700. The controller 700 stops the injection work of the injection pump 630 according to the information transmitted by the liquid level sensor 620 to complete the injection of the medium.

[0072] Example:

[0073] The culture container 200 can be placed into the incubator 100 through the cover 120 on the top of the incubator 100 for observation.

[0074] In their daily work, the staff can directly observe the approximate liquid level of the culture medium inside the test tube 210 in the culture container 200 through the glass 110 on the side of the incubator 100. When the liquid level of the culture medium is observed to be low or too low, the staff observes it through the touch screen 710 arranged on the top of the controller 700. The controller 700 controls the analysis mechanism 400 to control the multiple cameras 410 to photograph the test tube 210 from different directions, and the lighting lamp 420 provides supplementary light to the test tube 210 from the side, so as to help the camera 410 to photograph the test tube 210 more clearly.

[0075] The staff further analyzes the clear photos transmitted from the analysis mechanism 400 to the controller 700, observes the liquid level of the culture medium and compares it with the scale line 220 to obtain the accurate amount of culture medium injected. The staff inputs the obtained amount of culture medium injected into the controller 700 through the touch screen 710, and the controller 700 starts to control the whole operation.

[0076] The motion mechanism 300 starts to drive the culture container 200 to move, and the bottom of the test tube 210 of the culture container 200 is placed in the circular pit 360 of the rotating base 310. The rotating base 310 is connected to the support rod 330, driving the culture rack 340 connected to the support rod 330 to move together. The fixing hole 350 provided on the culture rack 340 corresponds to the circular pit 360 provided on the rotating base 310, and the test tube 210 is placed in the circular pit 360 through the fixing hole 350 to ensure that the test tube 210 will not shake at will when the rotating base 310 rotates, causing the test tube 210 to tilt. The transmission shaft of the motor 370 is connected to the rotating base 310, driving the entire motion mechanism 300 to move together.

[0077] The gear 320 is disposed on the transmission shaft of the motor 370 and contacts the rotation limit switch 510 of the calibration mechanism 500. The rotation limit switch 510 monitors the rotation speed of the rotating base 310 through the connection with the gear 320. When the speed of the rotating base 310 reaches the set maximum speed, the rotation limit switch 510 sends a signal to the controller 700 to stop the motor 370. The controller 700 controls the motor 370 to stop moving and moves the test tube 210 to the approximate injection position.

[0078] When the test tube 210 stops, the calibration mechanism 500 can detect the magnetic field changes of the rotating base 310 for the Hall sensor 520. By comparing the continuously read Hall sensor 520 signals, the position offset of the rotating base 310 relative to the infusion mechanism 600 is detected. The small power supply 530 arranged on the Hall sensor 520 provides operating power for the Hall sensor 520.

[0079] The controller 700 determines the distance the rotating base 310 needs to move based on the signal transmitted by the Hall sensor 520, controls the motor 370 to work, calibrates the position of the test tube 210, and helps the feed port 260 of the test tube 210 to align with the infusion tube 650 of the infusion mechanism 600.

[0080] The photoelectric sensor 610 of the infusion mechanism 600 further determines the position of the test tube 210, and determines whether there is a culture container 200 below by the returned distance value. If there is a culture rack 340 below, the reflected signal is fast and the distance is short; otherwise, the signal is slow and the distance is far, which means the culture container 200. If there is a culture rack 340 below, it means that the calibration is not completed, and the photoelectric sensor 610 sends a signal to the controller 700, which further controls the Hall sensor 520 to detect, and controls the motor 370 to adjust according to the data obtained by the detection until the culture container 200 is below the photoelectric sensor 610.

[0081] When the photoelectric sensor 610 determines that the culture container 200 is below, it sends information to the controller 700, and the controller 700 controls the injection pump 630 to drive the culture medium in the liquid storage tank 640 to be injected into the test tube 210 through the infusion tube 650. The culture medium enters the test tube 210 through the feed port 260 on the cover plate 250, first contacts the grooves 240 formed by the intersection of the cell culture plates 230 in the test tube 210, and then flows into the test tube 210. This is to avoid direct contact between the injected culture medium and the culture medium in the test tube 210, resulting in splashing of the culture medium, causing waste and instability, and causing damage to the cell culture environment in the test tube 210. The position of the groove 240 is lower than that of the culture rack 340 to prevent the detection of the photoelectric sensor 610 from being affected. The cell culture plate 230 is two pieces of material that can allow cells to grow by adhering to the wall, and they cross each other. The cell culture plate 230 contacts the bottom of the test tube 210 to improve stability, in order to increase the area in contact with the culture medium in the culture container 200. While providing more space for stem cells to grow by adhering to the wall, the embedded groove 270 set on the cover plate 250 fixes the part of the cell culture plate 230 that extends out of the test tube 210, and the cover plate 250 is connected to the mouth of the test tube 210. The cell culture plate 230 is further fixed to ensure that the cell culture plate 230 will not shake during the movement of the culture container 200, providing a stable growth space for stem cells.

[0082] When the liquid level sensor 620 disposed inside the liquid storage tank 640 detects that the amount of the output culture medium reaches the culture medium injection amount set by the staff, a signal is sent to the controller 700 to shut down the injection pump 630 and complete the culture medium injection.

[0083] After completing the injection work, the above structure repeats the above command to inject the culture medium into the next test tube 210 until all test tubes 210 have completed the injection of culture medium. The staff turns off the controller 700, stops the controller 700 from working, and completes the culture medium injection work of the entire culture container 200.

[0084] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A stem cell culture device, characterized in that: include: An incubator (100); the four walls of the incubator (100) are provided with glass (110), and the top is provided with a sealing cover (120); A culture container (200) is arranged inside the culture box (100) and serves as a place for cultivating stem cells; A motion mechanism (300) is disposed inside the incubator (100) and drives the culture container (200) to move in the incubator (100); An analysis mechanism (400) is disposed inside the incubator (100) and monitors the culture medium in the culture container (200); A calibration mechanism (500) is arranged on the motion mechanism (300) and calibrates the stop position of the motion mechanism (300); An infusion mechanism (600) is disposed on the incubator (100) and is used to add culture medium into the culture container (200); The controller (700) is arranged on the top of the incubator (100), receives signals from the infusion mechanism (600), the calibration mechanism (500), the analysis mechanism (400) and the motion mechanism (300), and completes overall control of the above mechanisms according to the received signals; a touch screen (710) is arranged on the top of the controller (700).

2. A stem cell culture device according to claim 1, characterized in that: The culture container (200) comprises a test tube (210) arranged inside the movement mechanism (300); a scale line (220) arranged outside the test tube (210); a cell culture plate (230) arranged inside the test tube (210); and a groove (240) arranged at the center of the cell culture plate (230); The cover plate (250) is arranged on the top of the test tube (210); the feed port (260) is arranged on the cover plate (250) and is concentric with the groove (240); and the embedding groove (270) is arranged on the cover plate (250) and corresponds to the cell culture plate (230).

3. A stem cell culture device according to claim 1, characterized in that: The motion mechanism (300) comprises a motor (370) arranged at the bottom of the outer side of the incubator (100); a rotating base (310) arranged at the bottom of the inner side of the incubator (100) and connected to the driving end of the motor (370); a gear (320) arranged on the side of the transmission shaft of the motor (370) and located at the bottom of the outer side of the incubator (100); a support rod (330) arranged on the rotating base (310) and located at the center of the rotating base (310); a culture rack (340) arranged above the rotating base (310) and connected to the support rod (330); a fixing hole (350) arranged on the culture rack (340); and a circular pit (360) arranged on the rotating base (310) and concentric with the fixing hole (350).

4. The stem cell culture device according to claim 1, characterized in that: The analysis mechanism (400) comprises a camera (410) arranged inside the incubator (100); and an illumination lamp (420) arranged inside the incubator (100); the signal output ends of the camera (410) and the illumination lamp (420) are connected to the signal input end of the controller (700).

5. The stem cell culture device according to claim 1, characterized in that: The calibration mechanism (500) includes a rotation limit switch (510) which is arranged at the bottom of the outside of the incubator (100) and connected to the gear (320); A Hall sensor (520) is arranged on a culture rack (340); a small power supply (530) is arranged on the Hall sensor (520); and the signal output ends of the rotation limit switch (510) and the Hall sensor (520) are connected to the signal input end of the controller (700).

6. The stem cell culture device according to claim 1, characterized in that: The infusion mechanism (600) comprises a liquid storage tank (640) arranged on the top of the incubator (100); a liquid infusion tube (650) arranged inside the incubator (100) and connected to the liquid storage tank (640); an injection pump (630) arranged above the liquid storage tank (640); a photoelectric sensor (610) arranged on the liquid storage tank (640); and a liquid level sensor arranged inside the liquid storage tank (640); the signal output ends of the photoelectric sensor (610), the liquid level sensor, and the injection pump (630) are connected to the signal input end of the controller (700).

7. A method for using the stem cell culture device according to any one of claims 1 to 6, characterized in that: The following steps are involved: Step A: the analysis mechanism (400) takes a picture of the height of the scale line (220) where the liquid level of the culture medium in the culture container (200) is located, and the obtained picture is transmitted to the controller (700) for identification; Step B: the controller (700) controls the motion mechanism (300) to drive the culture container (200) to move according to the information transmitted by the analysis mechanism (400); Step C: the calibration mechanism (500) sends a signal to the controller (700), and the controller (700) controls the motion mechanism (300) to stop working; the controller (700) further calibrates the position of the culture container (200) based on the detection result of the calibration mechanism (500); Step D: After the calibration is completed, the controller (700) controls the touch screen (710) to control the infusion mechanism (600) to complete the addition of the culture medium inside the culture container (200) according to the culture medium injection amount set by the staff.

8. The method for using the stem cell culture device according to claim 7, characterized in that: The step C comprises: Step C1, the rotation limit switch (510) sends a signal to the controller (700), and the controller (700) controls the motor (370) to stop working according to the signal sent by the rotation limit switch (510); Step C2, the Hall sensor (520) detects the current position of the culture rack (340) and sends an adjustment signal to the controller (700); Step C3, the controller (700) controls the motor (370) to calibrate the position of the culture rack (340) according to the adjustment signal sent by the Hall sensor (520).

9. The method for using the stem cell culture device according to claim 7, characterized in that: The step D comprises: Step D1, the photoelectric sensor (610) determines that the test tube (210) is below and then sends a signal to the controller (700) that the culture medium can be injected; Step D2, after receiving the signal from the photoelectric sensor (610), the controller (700) controls the injection pump (630) to start working according to the medium injection amount set by the staff; In step D3, the liquid level sensor (620) transmits information on the amount of culture medium injected set by the staff to the controller (700). The controller (700) stops the injection operation of the injection pump (630) according to the information transmitted by the liquid level sensor (620) to complete the injection of the culture medium.

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