Extraction process of soy isoflavone with high genistein and genistin content and application method of soy isoflavone in sow production

Abstract

The invention relates to the field of feed additives, and particularly discloses a process for extracting soy isoflavone with high content of genistein and genistin and an application method of the soy isoflavone in sow production. The invention relates to a process for extracting soybean isoflavone with high genistein and genistin content, which comprises the following specific steps: carrying out graded screening on non-transgenic soybeans, carrying out presoaking by using an alcohol mixed solution, carrying out coordinated treatment by using bacteria and enzymes to obtain a molasses sample material, adjusting the sugar degree of the molasses sample material to 50-60 degrees, and crystallizing; separating sugar from the crystallized material, taking out pasty isoflavone, crushing, cleaning and secondarily separating the pasty isoflavone to obtain sugar, ash and pasty isoflavone, and sequentially performing crushing, steam sterilization, vibration screening, hot air drying and secondary screening on the obtained pasty isoflavone to obtain soybean isoflavone; according to the application, the contents of genistein and genistin in the soy isoflavone are increased by combining all the process steps.

Description

Technical Field

[0001] The present application relates to the field of feed additives, and in particular to a soybean isoflavone extraction process with high genistein and genistin content and an application method thereof in sow production. Background Art

[0002] Soy isoflavones are an important class of physiologically active substances with weak estrogen activity, antioxidant activity, anti-hemolytic activity and antifungal activity. They can effectively prevent and inhibit the occurrence of various diseases such as leukemia, bone hyperplasia, colon cancer, gastric cancer and prostate cancer. It has been found that soy isoflavones contain 12 types, which are divided into free aglycones and bound glycosides. Different production and extraction processes have different main active ingredients of soy isoflavones. There are mainly daidzein or daidzein, genistein and genistin, among which genistein and genistin are the main functional active ingredients of soy isoflavones. At present, the extraction processes of soy isoflavones mainly include: solvent extraction, pressure fluid extraction and solid phase extraction. However, the extraction efficiency of the existing soy isoflavone extraction process is low, and the proportion of genistein and genistin in the extracted soy isoflavones is low.

[0003] Some studies have found that adding 5, 10, and 15 mg / kg of soy isoflavones to sows during lactation can increase feed intake and milk production, and reduce E. coli, but no analysis of the entire intestinal flora was done. At the same time, other existing studies have not added soy isoflavones throughout pregnancy and lactation, and the main active ingredients of the added soy isoflavones are low or unclear, and the intestinal flora and colostrum quality of sows have not been explored. Summary of the invention

[0004] In order to increase the proportion of genistein and genistin in the extracted soy isoflavones, and to better apply soy isoflavone products with high content of genistein and genistin in sow production, the present application provides a soy isoflavone extraction process with high content of genistein and genistin and its application method in sow production.

[0005] In the first aspect, the present application provides a soybean isoflavone extraction process with high genistein and genistin content, which adopts the following technical scheme: A soybean isoflavone extraction process with high genistein and genistin content comprises the following specific steps: Non-genetically modified soybeans are graded and screened as raw materials to obtain a first material, the first material is pre-soaked with an alcohol mixture, and then treated with bacterial enzymes at 40-70° C. to obtain a molasses-like material, the sugar content of the molasses-like material is adjusted to 50-60 degrees with water, and the molasses-like material is crystallized at 50-70° C.; The sugar is separated from the crystallized material, and the solid phase paste isoflavones is taken out, the paste isoflavones are crushed and cleaned, and the cleaned material is subjected to secondary separation to obtain sugar, ash and paste isoflavones for the second time. The paste isoflavones obtained for the second time are successively crushed, steam sterilized, vibration screened, hot air dried, and screened for the second time to obtain soybean isoflavones with high content of genistein and genistein.

[0006] By adopting the above technical scheme, the content of genistein and genistin in soybean isoflavones is increased by improving the heating reflux method and extracting soybean isoflavones in combination with bacterial enzymes. Using alcohol mixed solution for pre-soaking can increase the dissolution rate of isoflavones, dissolve lipids and proteins in part of soybeans, and make isoflavones easier to be enzymolyzed. Adjusting the sugar content in molasses-like materials to 50-60 degrees can promote the enrichment of isoflavones in molasses-like materials, and the subsequent crystallization process can further separate isoflavones and improve the extraction efficiency of soybean isoflavones.

[0007] Preferably, the steam sterilization temperature is 90-95° C. and the sterilization time is 5-10 min.

[0008] Preferably, the hot air drying temperature is 70-95°C.

[0009] Preferably, the bacterial enzyme is derived from Verrucomicrobia.

[0010] By adopting the above technical scheme, bacterial enzymes in the Verrucomicrobia, such as β-glucosidase, can hydrolyze the glycosidic bonds of isoflavones to release aglycones, and other enzymes such as proteases and polysaccharidase can decompose proteins and polysaccharides in soybeans, reduce the impurity content in soybean isoflavones, and increase the content of genistein and genistin in soybean isoflavones.

[0011] In a second aspect, the present application provides a method for applying soybean isoflavones with high genistein and genistin content in sow production, using the following technical solution: A method for applying soybean isoflavones with high genistein and genistin contents in sow production. The soybean isoflavones are used as feed additives for improving sow reproductive performance and milk quality, and the feed additives are added during the gestation period of the sow.

[0012] By adopting the above technical scheme, soy isoflavones with high content of genistein and genistin are added as feed additives during sow pregnancy, which can increase the number of live and healthy piglets born by sows and improve the level and quantity of immunoglobulins in colostrum and blood.

[0013] Preferably, the soy isoflavones are used as a feed additive for improving the antioxidant function, immunity and reproductive hormones of sows, and the feed additive is added during the gestation period of the sows.

[0014] By adopting the above technical scheme, soy isoflavones with high content of genistein and genistin are added as feed additives during sow pregnancy, which can increase the expression of amino acid transport genes in sows, reduce the expression of pro-inflammatory factors, and thus improve the antioxidant function, immunity and reproductive hormones of sows.

[0015] Preferably, the soy isoflavones are used as a feed additive for regulating the intestinal flora of sows, and the feed additive is added during the gestation period of the sows.

[0016] By adopting the above technical scheme, soy isoflavones with high content of genistein and genistein are added as feed additives during the gestation period of sows, which can increase the relative abundance of beneficial bacteria in the intestines of sows and thus regulate the intestinal flora of sows.

[0017] Preferably, the soy isoflavones are used as a feed additive for improving the health and antioxidant function of sow offspring piglets, and the feed additive is added during the sow's lactation stage.

[0018] By adopting the above technical scheme, adding soybean isoflavones with high content of genistein and genistin as feed additives to sows during lactation can reduce the diarrhea rate of offspring piglets to a certain extent.

[0019] In summary, this application has the following beneficial effects: 1. Since the present application adopts the heating reflux method in combination with bacterial enzyme to extract soybean isoflavones, the content of genistein and genistin in soybean isoflavones is increased, the dissolution of soybean isoflavones is promoted in advance with an alcohol mixture, and then the isoflavones are enzymatically hydrolyzed with bacterial enzymes.

[0020] 2. In this application, soybean isoflavones with high content of genistein and genistin will be added as feed additives during sow pregnancy and lactation to promote sow production. During pregnancy, it is beneficial to increase the number of live piglets and healthy piglets born by sows, increase the expression of amino acid transport genes, reduce the expression of pro-inflammatory factor genes, increase the level and amount of immunoglobulins in colostrum and blood, and increase the relative abundance of beneficial bacteria in the intestines of sows. During lactation, it is beneficial to reduce the diarrhea rate of offspring piglets and improve the health and antioxidant function of sow offspring piglets to a certain extent. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It indicates the effect of soy isoflavones on the α-diversity of intestinal flora in sows; Figure 2 To show the effect of soy isoflavones on the composition of intestinal flora in sows; Figure 3 It indicates the effect of soybean isoflavones on the relative abundance of dominant bacteria in the intestine of sows; Figure 4 It represents the species composition analysis of intestinal flora of sows; Figure 5 Represents the expression level of sow placenta-related genes. DETAILED DESCRIPTION

[0022] The present application is further described in detail below with reference to the accompanying drawings and embodiments.

[0023] All raw materials in the examples are commercially available. Example

[0024] Example 1 The present embodiment provides a soybean isoflavone extraction process with high genistein and genistein content, comprising the following specific steps: S1: using non-genetically modified soybeans as raw materials to remove metal foreign matter through a magnetic separator, and performing graded screening with a 50-mesh grading screen to obtain a first material, pre-soaking the first material with an alcohol mixed aqueous solution with an alcohol concentration of 75%, and then using a bacterial enzyme mixed synergistic treatment at 60°C to produce a molasses-like material, adjusting the sugar content of the molasses-like material to 55 degrees with water, and crystallizing the molasses-like material at 60°C to obtain a crystallized material.

[0025] S2: Separate the sugar from the crystallized material, take out the solid phase paste isoflavones, crush and clean the paste isoflavones, separate the washed material for the second time, obtain sugar, ash and solid phase paste isoflavones for the second time, crush the paste isoflavones obtained for the second time, steam sterilize at 93 ° C for 8 minutes during the crushing process, then use a 60-mesh vibrating screen to screen and remove impurities, then spray with a high-pressure pump, dry with 80 ° C hot exhaust air, and finally use a 60-mesh vibrating screen for secondary screening and packaging to obtain soybean isoflavones with high genistein and genistin content. The non-genetically modified soybeans used in this embodiment are produced in Northeast China, sown in late April to mid-May each year, harvested from September to October, and the bacterial enzymes are β-glucosidase and protease.

[0026] The soy isoflavone content prepared in this example was detected with reference to GB / T23788-2009 “Determination of Soy Isoflavones in Health Foods”. The detection results are shown in Table 1.

[0027] Table 1: Soy isoflavone content test results project Content (mg / kg) Proportion (%) Daidzein 1079.70 1.35 Soybean glycoside 13544.60 16.90 Genistein 454.10 0.57 Genistin 64503.00 80.48 Glycitein 53.90 0.07 The content of genistein and genistin in the soy isoflavone product prepared in the present application is about 454.1+64503.0 mg / kg, accounting for about 81% of the soy isoflavone product, which further illustrates that the extraction process of soy isoflavones in the present application significantly increases the content of genistein and genistin.

[0028] This embodiment also provides an application method of soybean isoflavones with high contents of genistein and genistin in sow production, including the following specific steps: Select 80 healthy two-way pregnant sows, and randomly divide them into 2 groups according to the principle of consistent parity and body condition: the control group and the experimental group, with 40 replicates in each group and 1 sow in each replicate. Sows in the control group were fed a basal diet from mating to parturition. On the basis of the basal diet, sows in the experimental group were premixed with 150 mg / kg of soybean isoflavones with high contents of genistein and genistin into the small feed in advance. The feed intake of sows during the entire experimental period was as follows: about 2.1 kg was fed from 0 to 7 days of early pregnancy; about 2.4 kg was fed from 8 to 60 days of pregnancy; 2.2 kg was fed from 61 to 90 days of pregnancy; 3.8 kg was fed from 91 days of pregnancy to parturition. Then, tests were conducted on the reproductive performance and milk quality of soybean isoflavones. The test results of reproductive performance are shown in Table 2, and the milk quality results are shown in Table 3.

[0029] Index determination: (1) Sow feed intake: Record the feed intake per ton of sows during pregnancy and lactation, and calculate the average feed intake of sows during pregnancy and lactation.

[0030] (2) Litter performance: To calculate the birth weight of live piglets per litter and the average weight of live piglets, record the weight of newborn piglets before consuming colostrum during parturition. After all placentas are discharged, record the number of piglets per litter, including the total number of births, number of live births, number of live births at birth, number of stillbirths, and number of mummified piglets.

[0031] (3) Colostrum quality: Collect about 20 ml of colostrum from the 3rd and 4th pairs of nipples on one side of the sow 12 hours after parturition. After gently mixing, store it at -20°C for determination of dry matter, milk fat, milk protein, lactose content, and immunoglobulin level.

[0032] Each sow was used as an experimental unit for statistical analysis. The SPSS 27.0 statistical software was used to conduct independent sample T-test statistical analysis on the data of the 2 groups. The normality of all data was tested. If the data did not conform to the normal distribution, the Kruskal-wallis test was used for analysis. The results were expressed as mean ± standard error. P<0.05 indicated a significant difference, and 0.05<P<0.10 indicated a trend.

[0033] Test results: Table 2: Effects of soybean isoflavones with high contents of genistein and genistin on the reproductive performance of sows As shown in Table 2, compared with the control group, the addition of soy isoflavones to the diet of pregnant sows can increase the number of live piglets by 1.5 and the number of healthy piglets by 0.9, and reduce the number of mummies. The average daily feed intake of sows in the soy isoflavones group during pregnancy was significantly higher than that in the control group. The addition of soy isoflavones to the diet had no significant effect on the back fat, total number of piglets born, number of weak piglets, weak piglet rate, number of stillbirths, stillbirth rate, live piglet weight born per litter, and delivery time of sows. This further shows that the application of soy isoflavones with high content of genistein and genistin during sow pregnancy can increase the number of live piglets and healthy piglets born by sows.

[0034] Table 3: Effects of high-content genistein and genistin soybean isoflavones on the nutritional composition and immunoglobulins of sow colostrum project Control group Soy isoflavone group P-value Milk protein g / 100mL 14.80±0.32 15.03±0.58 0.733 Lactose g / 100mL 3.13±0.21 3.25±0.11 0.635 Dry matter g / 100mL 25.62±1.12 26.48±1.25 0.617 Milk fat g / 100mL 5.47±0.61 5.95±0.89 0.662 Ig Gμg / mL <![CDATA[380.50±8.13 b ]]> <![CDATA[405.37±7.45 a ]]> 0.048 Ig Mμg / mL 40.80±0.76 40.38±0.80 0.610 As shown in Table 3, adding soy isoflavones to sow feed has no significant difference in the conventional nutritional components of sow colostrum. Adding soy isoflavones to sow feed can significantly increase the IgG content in sow colostrum.

[0035] Example 2 This embodiment provides a method for applying soybean isoflavones with high genistein and genistin content in sow production. The test animals are the healthy and grown binary hybrid multiparous sows in Example 1, and the test location and test method are the same as those in Example 1. Soy isoflavones were tested on the antioxidant function, immunity and hormones of sows. The results of the antioxidant function test are shown in Table 4, the results of the reproductive hormone test are shown in Table 5, and the results of the immunity level test are shown in Table 6.

[0036] Index determination: (1) Antioxidant capacity: On the 35th and 85th days of pregnancy and the 14th day of lactation, blood was collected from 6 sows randomly selected from each group and serum was collected for storage to prepare for the determination of serum antioxidant indicators, including malondialdehyde, glutathione peroxidase GSH-Px, superoxide dismutase SOD, catalase CAT and total antioxidant capacity TAOC, using kits purchased from Nanjing Jiancheng Bioengineering Institute. All assays were performed according to the manufacturer's procedures and were performed in duplicate.

[0037] (2) Immune capacity: On the 35th and 85th days of gestation and the 14th day of lactation, blood was collected from 6 sows randomly selected from each group and the serum was collected and stored for the determination of serum concentrations of immunoglobulin A, IgG, IgM, interferon-γ, interleukin-1, IL-6, IL-10 and tumor necrosis factor-α using ELISA kits. All immunoglobulin and cytokine analyses were performed in duplicate according to the manufacturer's instructions.

[0038] (3) Serum hormones: On the 35th day of gestation, the 85th day of gestation, and the 14th day of lactation, six sows were randomly selected from each group to collect blood and preserve the serum for measuring the levels of serum estradiol E2, progesterone P4, leptin, and insulin-like growth factor. The enzyme-linked immunosorbent assay (ELISA) kit was used for measurement, and all serum hormone analyses were performed in duplicate according to the manufacturer's instructions.

[0039] Data processing and analysis: Each sow was used as an experimental unit for statistical analysis. The experimental data were statistically analyzed by independent sample T-test for the two groups of data using SPSS 27.0 statistical software. All data were tested for normality, and those not conforming to the normal distribution were analyzed by Kruskal-wallis test. The results were expressed as mean ± standard error, and P < 0.05 indicated significant difference, and 0.05 < P < 0.10 indicated a trend.

[0040] Table 4: Effects of soybean isoflavones with high contents of genistein and genistin on the serum antioxidant function of sows As shown in Table 4, adding soybean isoflavones with high contents of genistein and genistin to the sow diet significantly increased the serum CAT level of sows on the 35th day of gestation, increased the immunoglobulin G in the blood by about 12 μg / ml, and there was a trend of increasing the T-AOC ability of sows on the 85th day of gestation. It further indicated that soybean isoflavones with high contents of genistein and genistin were beneficial to increasing the level and amount of immunoglobulins in the blood.

[0041] Table 5: Effects of soybean isoflavones with high contents of genistein and genistin on the serum reproductive hormone levels of sows As shown in Table 5, adding soybean isoflavones with high contents of genistein and genistin to the sow diet had a trend of decreasing estradiol in sows on the 14th day of lactation, and had no significant effect on estradiol, leptin, progesterone, and insulin-like growth factor at other stages. It further indicated that soybean isoflavones with high contents of genistein and genistin were beneficial to regulating the reproductive cycle in sow production, maintaining normal follicular development and ovulation, and increasing the litter size and piglet survival rate.

[0042] Table 6: Effects of soybean isoflavones with high contents of genistein and genistin on the serum immune indexes of sows As shown in Table 6, adding soybean isoflavones with high contents of genistein and genistin to the sow diet had a trend of increasing the IgG level of sows on the 85th day of gestation, and had no significant effect on the levels of IL-1, IL-6, IL-10, IgA, IgM, IgG, TNF-α, and IFN-γ in the serum at other stages.

[0043] Example 3 This embodiment provides a method for applying soybean isoflavones with high genistein and genistin content in sow production. The test animals are healthy binary hybrid multiparous sows in Example 1. The test location and test method are the same as those in Example 1. The soybean isoflavones are tested on the intestinal flora of sows. Figure 1 The effect of soy isoflavones on the α-diversity of intestinal flora in sows was shown. Figure 2 , Figure 3 and Figure 4 It can be seen that soy isoflavones affect the relative abundance and dominant flora of sows' intestinal flora.

[0044] Index determination: At the end of the experiment, 5 g of fresh feces from sows were collected, quickly frozen in liquid nitrogen and stored in a refrigerator at 80°C for the determination of 16S diversity of fecal flora.

[0045] Total genomic DNA was extracted using Tiangen Bio's DNA extraction kit DP328. The integrity and concentration of RNA were detected using NanoDrop ND2000-Thermo. According to the target fragment, PCR was amplified to the V3-V4 region of 16SrDNA, 341F-(5'-CCTAYGGGRBGCASCAG-3') and 806R-(5'-GGACTACNNGGGTATCTAAT-3'). Then the PCR product of the 400-450bp fragment was extracted by 1.5% agarose gel electrophoresis and purified using the GeneJET gel extraction kit (Therm). The library was established by the Thermo-Ion Plus Fragment library Kit. After library testing, each duplicate 16SrDNA was pooled on the Jime Bio IonS5TMXL sequencing platform for paired-end sequencing.

[0046] Data processing and analysis: Based on the IonS5TMXL sequencing platform, FLASH (V1.2.7) was used to filter the quality of the original tags and extract valid tags. All valid tags were clustered into operational taxonomic units (OUTs) with 97% homology similarity using Uparse software (V7.0.1001), and these sequences were annotated using the SSUrRNA database. Alpha diversity and beta diversity were analyzed based on the OTUs level.

[0047] Test results: Depend on Figure 1 It can be seen that the addition of high-content genistein and genistin soy isoflavones in the diet has no significant effect on the α diversity of the intestinal flora of lactating sows. Figure 2It can be seen that the microbial composition of the soybean isoflavone group with high content of genistein and genistin added to the diet is quite different from that of the control group. Figure 3 and Figure 4 It can be seen that through LEfSe analysis, the LAD threshold was 2. At the genus level, the relative abundance of three dominant bacteria, Closteridia, UCG-004 and Papillibacter, in the intestines of sows in the soy isoflavones group was significantly higher than that in the control group, further indicating that soy isoflavones with high content of genistein and genistein are beneficial to improving the relative abundance of intestinal flora in lactating sows.

[0048] Example 4 This example provides a method for applying soybean isoflavones with high genistein and genistin content in sow production. The test animals are piglets born by healthy binary hybrid multiparous sows in Example 1. The test location and test method are the same as those in Example 1. The effects of soybean isoflavones on sow placenta-related genes were tested and the test results are shown in Figure 5 .

[0049] Index determination: After delivery, 6 sows were randomly selected from each group to collect placental tissue. The detection steps and reaction system were referred to TB Premix Ex TaqTMII (Takara) reagent instructions, detection of placental tissue glutamate cysteine ​​ligase modification subunit gene GCLM; fatty acid transporter 1 (FATP1); glucose transporter genes SLC2A3 and SLC2A13; amino acid transporter genes SANT1, SNAT2, SNAT4 and SLC38A10; insulin-like growth factor-1 (IGF-1); placental imprinted genes IGF-2 and H19; placental proinflammatory cytokine genes IL-1β and TNF-α; antioxidant gene GSH-Px transcriptional abundance. Primers refer to the NCBI database, and the target gene sequence primers are synthesized by Shanghai Bioengineering. The primer and probe sequences are shown in Table 7. The reaction system is 10μL, including TB Green premix Ex TaqⅡ5μL, forward primer 0.4μL, reverse primer 0.4μL, ROXReference0.2μL, DEPC2μL, cDNA 2μL. The internal reference gene β-actin was used as the internal reference gene, and the 2-ΔΔCT method was used to calculate the gene expression levels of the two groups.

[0050] Table 7: Primer and probe sequences Data processing and analysis: Each sow was used as an experimental unit for statistical analysis. After the experimental data were preliminarily sorted out using Excel software, independent-sample T-tests were performed on the two groups of data using SPSS 27.0 statistical software. All data were tested for normality, and for those not conforming to a normal distribution, Kruskal-wallis tests were used for analysis. The results were expressed as mean ± standard error, with P<0.05 indicating significant differences and 0.05<P<0.10 indicating a trend. The figures were drawn using GraphPad prism 8.3 software.

[0051] Experimental results: As Figure 4 shown, soy isoflavones with high contents of genistein and genistin can significantly reduce the expression levels of IL-1β and H19 mRNA in the placenta; however, they increase the expression levels of key imprinted genes such as SNTA2, SNAT4, and IGF-1 mRNA. Nevertheless, there were no significant differences in the gene expression levels of the nutrient transporters SLC2A3, SLC2A13, SLC38A10, and FATP1 between the two groups.

[0052] Example 5 This example provides a method for applying soy isoflavones with high contents of genistein and genistin in sow production. The experimental animals were the piglets born to healthy crossbred multiparous sows that grew up in Example 1. The experimental location was the same as in Example 1. The experimental method was to add 200 mg / kg of soy isoflavones to the soy isoflavone group, and the growth performance and antioxidant function of the piglets of the sows' offspring were tested for soy isoflavones. The test results are shown in Table 8.

[0053] Index determination: (1) Five days after parturition: Record the weight of each litter of piglets, ensuring that the number of piglets per sow is close. If there are differences, the number of piglets carried by the sow can be adjusted within the same group.

[0054] (2) Twenty-one days after parturition: Record the weight and number of each litter of piglets. Keep records of fostering and culling during the experiment, including the date and weight. Calculate the daily weight gain from 5 to 21 days based on the initial and final weights and the number of piglets in each pen.

[0055] (3) Piglet diarrhea rate: Record the number of diarrheal piglets per litter per day from 5 to 21 days, and calculate the piglet diarrhea rates of the control group and the functional package group.

[0056] Diarrhea rate (%) = sum of the number of diarrheal piglets per day in the experimental piglets / sum of the daily inventory of the experimental piglets * 100 (4) Piglet survival rate: Record the number of dead piglets per litter during the period from 5 to 21 days, and calculate the piglet survival rates of the two groups.

[0057] Survival rate (%) = number of experimental piglets per litter at 21 days / number of experimental piglets per litter at 5 days * 100.

[0058] Data processing and analysis: Each sow was used as an experimental unit for statistical analysis. After the experimental data were preliminarily sorted out using Excel software, independent-sample T-tests were performed on the two groups of data using SPSS 27.0 statistical software. The normality of all data was tested. If the data did not conform to the normal distribution, Kruskal-wallis tests were used for analysis. The results were expressed as the mean ± standard error. A P value < 0.05 indicated a significant difference, and 0.05 < P < 0.10 indicated a trend.

[0059] Table 8: Effects of soy isoflavones with high contents of genistein and genistin on offspring piglets project Control group Soy isoflavone group P-value Average number of piglets / head 10.89±0.17 11.23±0.15 0.140 Litter weight gain / kg 30.23±1.27 29.99±1.34 0.896 Average weight at 6 days old / kg 2.21±0.10 2.06±0.09 0.271 Average weight at 17 days old / kg 4.98±0.17 4.82±0.15 0.276 ADG / g 251.95±9.47 242.60±8.98 0.476 Diarrhea rate / % 1.00±0.33 0.35±0.12 0.064 Survival rate / % 98.49±0.76 98.89±0.53 0.659 SOD U / mL 223.56±13.83 221.69±18.48 0.937 GSH-PX U / mL 418.81±41.16 480.41±39.07 0.303 CAT U / mL 3.10±0.48 3.96±0.93 0.428 MDA nmol / mL 6.78±0.33 7.62±0.65 0.275 As can be seen from Table 8, adding soy isoflavones with high contents of genistein and genistin to the sow diet can reduce the diarrhea rate of offspring piglets; however, it has no significant effect on the number of piglets carried by sows, as well as the litter weight gain, weaning weight, average daily gain (ADG), and survival rate of offspring piglets. It has no significant effect on SOD, CAT, MDA, and T-AOC.

[0060] This specific embodiment is only an interpretation of the present application and does not limit the present application. After reading this specification, those skilled in the art may make modifications to this embodiment without creative contributions as needed, but as long as they are within the scope of the claims of the present application, they are protected by the patent law.

Claims

1. A soybean isoflavone extraction process with high genistein and genistin content, characterized in that: The specific steps include: Non-genetically modified soybeans are graded and screened as raw materials to obtain a first material, the first material is pre-soaked with an alcohol mixture, and then treated with bacterial enzymes at 40-70° C. to obtain a molasses-like material, the sugar content of the molasses-like material is adjusted to 50-60 degrees with water, and the molasses-like material is crystallized at 50-70° C.; The sugar is separated from the crystallized material, and the solid phase paste isoflavones are taken out. The paste isoflavones are crushed and cleaned, and the cleaned material is separated for the second time to obtain sugar, ash and solid phase paste isoflavones. The paste isoflavones obtained for the second time are crushed, steam sterilized, vibration screened, hot air dried, and screened for the second time to obtain soybean isoflavones with high content of genistein and genistin.

2. The soybean isoflavones extraction process with high genistein and genistin content according to claim 1, characterized in that: The steam sterilization temperature is 90-95° C. and the sterilization time is 5-10 minutes.

3. The soybean isoflavones extraction process with high genistein and genistin content according to claim 1, characterized in that: The hot air drying temperature is 70-95°C.

4. The soybean isoflavones extraction process with high genistein and genistin content according to claim 1, characterized in that: The bacterial enzyme is derived from the phylum Verrucomicrobia.

5. A method for using soybean isoflavones with high genistein and genistin content as claimed in any one of claims 1 to 4 in sow production, characterized in that: The soy isoflavones are used as a feed additive for improving the reproductive performance and milk quality of sows, and the feed additive is added during the gestation period of the sows.

6. The method for applying soybean isoflavones with high genistein and genistin contents in sow production according to claim 5, characterized in that: The soy isoflavones are used as a feed additive for improving the antioxidant function, immunity and reproductive hormone of sows, and the feed additive is added during the gestation period of the sows.

7. The method for applying soybean isoflavones with high genistein and genistin contents in sow production according to claim 5, characterized in that: The soy isoflavones are used as a feed additive for regulating the intestinal flora of sows, and the feed additive is added during the gestation period of the sows.

8. The method for applying soybean isoflavones with high genistein and genistin contents in sow production according to claim 5, characterized in that: The soy isoflavones are used as a feed additive to improve the health and antioxidant function of sow offspring piglets, and the feed additive is added during the sow lactation stage.

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